(ame pou N\ QveOle er satan 0 beocQewen of pa clove onw cout oCate 2) Wer gatomt brobeges Vv cm be pra tice ede Coplente zgo bate de Kahu Femjome ae gare le cou Junta & yaranor de ptt plow he ar roolumow® ae atcde (e4 yy, eudlo ates ) L bor (oA us wu, )- ( qc atae Tews [ome we rupee ae {use bpvo€e gee gous ch fofets) cL Dimounts , gts eaninnerie 6 rtucot , (Confauts a oput bei? Jone of) (permeers Ute wader)» juihrout Ub) 2) Vexowon, Adicboate oe Ne gous uti tml © Comfeegate ye bo wrdio swe Uine fel reek, ca mole nye teemer he aferatcve- ——— fede debe ou > RCO” a We 2 Caceo"t [oI A coon — pare oeuger® “ Lecoc¥] te GRRL cap - Gg CUA] = Cape Be * Laced (w= ke gol] = “Capea * ea Lvase cong ts} feedo] Bquentour oly I Hpac Haren dare debske H sual expom — Hemel bach ad pits phy + bye pi phe + e910 Chere P acide Couiupeto|Cayeate Tonheue Ce quantita Li 9 meamavia pu fet veden cl pW dé une ere mm fee B= db dove ACp uc Liaumule de pk cu a " egginmte do bee bb .! cu,coon ——> yer C00 He Clycac”ait? oy 1,0 opel” 5. Hoo Tews coo" J be Mon, cool] = olk= pRa + beg. i [ety cow] o> P Ra marine ca laettel tee ewe 1 La baroue fanjonaure Comune med utew wlho pha t4 2\ Hagarore Le fa teucutrarour Tota G magyeere oF fa. or}ect ter ce ee4.11. Carve di ttolazione di try acid deboli: acido acatico, HsPOz @ NHz. Lo for- te ionic predominant ad aunt pH sono ‘iportate neg insert. La regioni con.cape- cia tamporente soa0 le part piste: delle curve. Le cats Cotesia one ‘amponi efficient! quando la concentrazion: | Regiont el donators proton 9 tra i 23 ad 278 + tmpocent Der conto det totale 7 | NH; Cara Wer atte Ad we bueen Temleme tL A) buome cafe te Tamer pH Fosfato mdb! catiwalle Je pu video + 2) saleby bite’ cm W20 a 3) gto: Lite Ko — 4) mow anordin Le law Sueno __ | met yorn & 2 mt wv. ok i _ Equivalenti di CH" —> 3) mom culate pu ~ _20. pH and Buffers Revised by H. A. MCKENZIE (Department of Phys: <1 Biochemistry, Australian National University, Canberra) Physiological Media Revised by R. M.C, DAWSON The standardization and measurement of pH General references Notes on pH measurement Notes on the properties of buffer solutions Buffer capacity (buffer value) Dilution values Sait effects Temperature effects Metal ion effects Revised N.B.S, standard buffer solutions for pH measurements from 0° 10 95° Primary standards Secondary standards Approximate pK values of substances useful for buffer systems Buffer solutions for pH 1-13 1, Clark and Lubs, pH 1-0-22 2. p-Toluene sulphonic acid-sodium p-toluene sulphonate, pH 1-2-20 3. Glycine-HCI, pH 22-36 4. Clark and Lubs, pH 22-40 5. Citric acid-Na,HPO, (Melivaine), pH approx. 26-76 6. Universal (Britton and Robinson type), pH 26-120 7. Citric acid-sodium citrate, pH 306-2 8. B:8"-Dimethylglutaric acid-NaOH, pH 32-76 9. 6 ). Sodium acetate-acetic acid, pH 3 10. Succinic acid-NaOH, pH 38-60 IL, Clark and Lubs, pH 41-59 12. Sodium cacodylate-HCl, pH $-0-7-4 13. Sodium hydrogen maleate-NaOH, pH 5-2-6 14, Na,HPO,-NaH.PO,, pH 53-80 15. Clark and Labs, pH'5-$-8-0 16. Sodium bicarbonate-5% CO,, pH 6-0-8:0 17. Imidazole (glyoxaline)-HCL, pH 62-73 18. 2:4:6-Trimethylpyridine (2:4:6-collidine)-HCl, pH 6-485 19. Triethanolamine hydrochloride-NaOH, pH 638-6 20. Sodium 5:5-diethylbarbiturate (veronal sodium: barbitone sodium)-HCl, pH 68-96 . Dimethylleucyiglycine, pH 7-0-88 N-Ethylmorpholine-HCl, pH 70-32 Tris(hydroxymethyl)aminomethane, pH 7-1-8:9 . 2-Amino-2-methyl-1:3-propanediol-HCI, pH 73-97 5. Diechanolamine-HCl, pH $-0-100 5. Clark and Lubs, pH $-0-10-2 Borate, pH 1-9-0 . Glycine-NaOH, pH 86-106 437 483, 483 439 490 490 491 491 492 492 493 493 498, 494 495 496 496 41520. pH, Buffers, and Physiological Media 29. Sodium carbonate-sodium bicarbonate, pH 9-2-108 30. dorate, pH 9-107 31. Carbonate, pH 97-109 32. Phosphate, pH 110-119 : 33. Hydroxide-chloride, pH 12-0-13-0 Bufers of constant ionit strength |. HICLKCI mixtures of constant ionic strength (I), pH0-22 (2. Constant ionic strength burfer solutions of Miller ant Golder for electrophoresis, pH 20-12-0 3. Mellvaine type buffers of constant ionic strength (especially suitable for polarography), pH 2-2-3-0 4. Amine buffers of constant ionic streagtht 3. Buffers of low ionic strength for spectrophotometric pX determinations, pH 16 Buffers for electrophoresis 1, Univalent burfer systems for electrophoresis 2, Buffers suitable for paper electrophoresis of proteins 3. Butfers for starch gel elecstophoresis 4, Volatile buffers for high voltage electrophoresis, 5. Typical volatile buffer mixtures Approximate pH of some common reagents at room temperature Physiological media L. Krebs mammalian Ringer solutions 2 Other Ringer solutions 3. Artificial sea water 505 503 505 506 507 507 Tapella 4.1 | pK. di acidi e basi di uso comune per la preparazione di tamponi. Acido 0 base 9K. (a 25°C) ‘Acido ac ico 475 Acide barbiturico ‘Acido carbonico 0.22. Acide citnco 3.10; 4,76; 5.40 Gilictigiteina HEPES" 750 Acido tostorico 1,96; 8.70; 12,30 PIPES"> 620 ‘Acido ftatico 2.90; 5.51 Acide succinico 418; 5.56 2396 4.16 ans14 13 12 u— 10 pH a i NH,CHRCOO“}-—— —— Stadio 1. titolazione~~ del gruppo —COOH NH,CHRCOO- |NHsCHRCOOH| INH;CHRCOO7| pHi = 6.02 =p 4-{Su,crncoo’ — Forma isoelettrica 7 NHsCHRCOOH dipolare ! INH,CHRCOO™ | | Stadio 2, titolazione_ p . 7 del gruppo NH; Equivalenti aggiunti di 0,1 NaOH Coal bk! ddolagcare dat’ elas 0.44 4 et t pkas eh, oka, € 3 Cie Ni, £3 eh ey > ce nie 2.34 63 i eBoy oo C00 Cowen whe, Conta wala © Corre valle, eer be (lone. depdow) mefoh ¢ We = poss gor = 6.04 2Coon C00 Coo~ Can Che pts, ore, Ha, oe Ke eh ' => } a rm oy Che i ve a Te e— bh . . y Canny ch-wihs thm" 3.62 CN. . = Coo 4 C00" ‘ *° Cole dipolas pts 3.22 ferme + + ne Ni Net Nev du. Kar ct, ghz dh Kay dae Ges

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