The experiment with mold and bread

Introduction
Mold is a fungus. Its main needs to survival are water, air and
food. Like humans. Mold isnt green so sun is not needed for it
to grow. The mold grows in indoor area. environment needed for
mold to grow has oxygen, water, shelter and food. So we are
having an experiment to see if the more amount of water we put
on the bread, will make the mold grow bigger. Mold will grow one
old food that is moist and it is in a closed or indoor place. The
mold will take one more week to grow.
Research question
Will the amount of water we put on the bread effect the size of
the mold larger or smaller.
Hypothesis
If we put more water on the bread which is the more amount of
sprays
Then the mold will grow larger and there will be more pieces of
mold on the bread.
Mold needs to live in a moist place for it to grow and to live. Mold
will grow bigger when there is more water because if it is moister
then the mold will grow more. When it has more water the water
will spread more and the mold will follow the water and spread
more. And I think that there will be more pieces of mold
(colonies of mold) because we put the mouth on mist and there
will be more drops which causes more pieces of mold (colonies
of mold) In my predictions the mold will grow bigger because
when we spray the water then the water level would get thinker
and would get wider for the mold to grow which then would help
the mold grow bigger the more amount of water.
Variables
Independent
variable

The amount of
water we put
on the bread in
the
independent

The first piece of bread I will

spray water once, the
second piece of bread I will
put 2 spray the third will
have 3 spray, the fourth will

variable.

get 4 spray, the fifth will

have 5 spray, the sixth will
get 6 spray.

Dependent
variable

The growth of
the mold is the
dependent
variable.

After one week the bread

have been in the bag I will
measure the mold in the
middle I will find the fattest
part of the mold I will
measure the diameter.
Remember that the
diameter is the width of
something.

Controlled
variables

The things that

we keep the
same in the
whole
experiment

The same type of

water (water source)
(just use tap water
dont use two water
source)
The same spray bottle
(the same mouth.
(Keep it as mouth as
mist) you have to not
touch the mouth and
always pull the trigger
fully dont pull it half
way you have to fill it
fully.
The same ruler (cm
ruler use the same
ruler to measure for
all of the breads)
Same type of bread
( use the bread in the
same packet dont
take one piece of
bread from one packet
and the other piece
from another packet
because each place
has a different way of
making bread and

they might use

different ingredient)
Dont touch the mouth
of the spray while
doing the experiment
Pull the trigger fully to
not mess up the
experiment (pull the
trigger till the end
dont pull only half or
one part of it pull it
fully)
Materials: What did you use?
1. 6 pieces of bread ( any type but all six has to be from the same
package )
2. 6 ziplock (closes ziplock )
3. 1 ruler ( normal class (cm) ruler )
4. 1 spray bottle ( 6 full spray )
5. Water ( tap water, but if you use another type of water then use
it for all the other pieces of bread )
6. A pair of gloves
7. 6 labels (you will need it to use this to know which one is yours
and how many sprays you put on each piece of bread)

Method:
1. First take all your materials including:

2.
3.
4.
5.
6.
7.
8.

Type of bread (use any type bread but all six has to be
from the same package)
6 zips lock (closes zip lock)
1 ruler (normal class (cm) ruler)
1 spray bottle (6 full spray)
Water (tap water)
A pair of gloves
Second put the zip-lock straight on the table
Then put the bread on top of the zip-lock
Fourthly put water from the sink and fill up the spray bottle
Fifthly using the spray bottle, spray once on the bread on the
side facing the sealing (strong reminder: pull the trigger
fully)
Then put on your gloves
Then hold the bread and put it into the zip-lock
Zip the zip-lock

9. Do that to the other 5 pieces of bread ( but remember: The

first piece of bread I will spray water once, the
second piece of bread I will put 2 spray the third will
have 3 spray, the fourth will get 4 spray, the fifth will
have 5 spray, the sixth will get 6 spray)
10.
label the bags, label it to the amount of sprays you
put and your name
11.
After 1 week of the six pieces of bread in the zip-lock
you will measure the mold using the ruler (measure the
middle of the mold (the fattest part of the mold
diameter) remember the diameter)
12.
Measure the other 5 pieces of bread the rest.
Data
No. sprays

Measuremen No.
t of colony
colonies ( C
size ( cm )
)

#1
0 cm

0c

1 cm

1c

1 cm

1c

0.5, 1.8 , 0.4, 0.6

4c

0.5, 0.5, 0.5, 0.5,

0.8, 0.4

6c

1.2, 0.5, 0.8, 1,

0.7,0.5, 0.7, 0.8,
0.3
If there is more
water than the
size of the mold

9c

#2

#3

#4

#5
#6

Observation
of all

To me if we put
more water
there is more

Observation
about each
bread
This piece of
bread has no
mold on the
bread
this piece of
bread has only
one colony of
mold
This piece of
bread has only
one colony of
mold
This bread has
colonies of mold
3 small and one
big
This bread has
up to 6 colonies
of mold
This bread has 9
colonies of mold
in it
To my
observation if
we put more

will be bigger

colonies of
mold

water in the
bread then there
will be more
mold on the
bread.

Graph

Number of Colonies

No. colonies

10
9
8
7
6
5
4
3
2
1
0
1 spray

2 spray

3 spray

4 spray

5 spray

6 spray

No. sprays

Analysis
This graph shows how many colonies there are with an amount of
water. With 1 spray there are no colonies of bread, 2 sprays there
is one colony of bread and with 3 spray also one colony with 4
sprays there are up to 4 colonies of mold, with 5 sprays there are
6 colonies and with 6 there are 9 colonies of mold. Its predicted
that the reason that we have more colonies of mold is because
when we spray more than the water spreads more which then

makes more colonies of mold, and its because the mouth of the
spray was in mist which means there are lots of little drops and
which means that the drops will go everywhere and makes more
colonies of mold. The hypothesis supported by data because my
hypothesis says that when there is more water then there will be
more mold colonies and that was exactly what happened.
Conclusion
1. Was your hypothesis accepted or rejected?
2. Explain you rational for rejecting of accepting the
hypothesis?
The hypothesis predicted that (If we put more water on the
bread which is the more amounts of sprays then the mold
will grow larger and there will be more pieces of mold on
the bread) The hypothesis is accepted because my hypothesis
was correct as we can see in the graph says that the more
amount of sprays on the bread gave the bread more colonies of
mold. But with the more amount of bread it did not exactly make
the molds bigger but it did make more mold colonies. The Data
was highly close to the hypothesis and the method was very valid
the method showed exactly how we did it and it showed. It is sure
that in this experiment there will be some inconsistency, in some
of the answers and that may be because it might have been hard
to pull the trigger fully because not every single spray since our
hand cant control every single spray so there might be some
miner mistake but it will not and should not cause any bad results
to this experiment. But there was one way to keep the sprays
consistence is to pull the trigger for all the water to go out after
the spray to not leave out any water and it would be at the least
90% consistence and time the prays so every single spray will
have the same amount of time to let the water out.
My hypothesis is mostly correct because it was predicted that (If
we put more water on the bread which is the more amount of
sprays then the mold will grow larger and there will be more
pieces of mold on the bread) and the prediction was mostly
correct we said there will be more colonies and the mold will be
bigger and most of that was what happened there were more
colonies of mold but the molds werent exactly bigger than each
other so part of the hypothesis was correct. And the reasoning
that the mold would have colonies because there are more drops

around it that is why the more sprays we give it then there will be
more colonies of mold.
Evaluation
- Was the method valid (Were you able to control the
variables)
- How would you improve your method? (Explain why)
I think that the method was very valid because the method
was very correct and the variables were controlled very well
and the experiment was kept very stable and the experiment
was kept very controlled and the experiment went very well. I
think that this method could not be changed much but that
says to put on your gloves that werent very useful. And the
rest is very good and it seemed to work very well for the
experiment and the answers were mostly correct but I will also
make a miner change step: I would add this to step (5) (when
you pull the trigger pull it fully and let all the water
finish dropping before you let the trigger go) I would
improve that because in the experiment the hardest thing was
being able to keep all the sprays the closest as possible and
we try to make all the spray to be mostly the same.
Further research
Describe 1 or more improvements that would benefit the
investigation
The improvement could have done is to have a more
accurate water transfer because the one we used in this
experiment was quite inaccurate in a way so we could have
use another item instead of this water transferor.
Describe a further investigation that would help you to
answer the research question more accurately.
I think that if we had more investigation then we could have
found out more about how the mold grew and in what ways
did the mold have air to grow in the zip-lock because as the
introduction said before mold in a fungus it uses water air
and food, so in what way did it grow if the zip-lock was
closed and had not much air inside. Or maybe we could
make more tries and see what would happen if we changed

the mouth rather being mist the it could be changed to

straight spray.