Food and Digestion Lab Answer Sheet

Robert Ramirez
Bio 011

Key Organs and Enzymes of the Digestive System

1) The salivary glands produce salivary amylase to digest __________carbs______.
2)

The stomach produces pepsin, which is a protease to digest

______protein_______________________.

3) The pancreas produces:

Proteases, to digest _________protein______________ within the small intestine
Pancreatic amylase to digest ___________carbs____________ within the small intestine
Pancreatic lipase to digest _______________lipids_________________ the small
intestine
Part 1: Testing for Macromolecules
Hypotheses

Food 1 (Healthy):egg

Food 2 (healthy):apple

Food 3 (healthy):cheese

Food 4 (junk):
Tuna

Food 5 (junk):funions

Food 6 (junk): noodles

Directions for Testing

1) Why is it important to use a negative and positive control?
It is used to keep track of which solutions are positive and negative

2) Use the following table to record your results for all 4 tests. Record both the color of
the resulting solution and whether the test is + or - for the molecule being tested.
Food

Benedicts

Iodine

Biuret

Sudan IV

(simple sugar)

(starch)

(proteins)

(lipids)

Color

+ or -

Color

+ or -

Color

+ or -

Color

Positive
Control

--

Red

Black

Purple

cloudy red

Negative
Control

--

Blue

Brown

Colorless

full red

Food 1

Egg

Blue green

Brown

Pink purple

Cloudy white

Food 2

Apple

Red

Brown

Yellow

light pink

Food 3

Cheese

Blue

--

Dark brown

Purple

Pink

Food 4

Tuna

Blue

Brown

Pink purple

Cloudy white

Food 5

Funions

Blue

Black

Light yellow

yellowish

Food 6

Noodles

Slight green
blue

Very dark
green

Blue

colorless

3) What conclusions can you draw about the nutrients found in the foods you tested?

There were a variety of ingredients within our food products and we're concluded that the
concentration of lipids and sugars within our food was extremely high.

Part 2: Digestion of Food

1) Fill in the following tables based on your results in lab
S1

S2

S3

Natural Color of test tube

White

White

Gray

Iodines Reaction color

Clear
Yellow

Clear
orange

Foggy
yellow

Is there presence of starch?

Y/N

No

Yes

No

G1

G2

G3

G4

Natural Color of test tube

White

White

Gray

White

Benedict's Reaction color

Light
Blue

Light
Blue

Light
Green

Dark
Orange

Is there presence of
glucose? Y/N

No

No

Yes

Yes

2) Which tubes successfully digested starch?

S2 g3 g4
Part 3: Post-Lab thought experiment

Unknown-S

Unknown-G

1) Billy was super hungry after class, so he stopped at In-n-Out and ordered a #3 (Doubledouble, fries, and a strawberry shake). As he is eating he wonders how his tasty meal is
being digested. Using the word bank below, create a diagram showing Billy how his meal
moves through his digestive tract (from mouth to anus) and how his body works to
digest it.
Mouth

Salvia

Amylase

Esophagus

HCl

Pepsin

Stomach

bile

Large intestine

Pancreas

Small intestine

Gastric juices

Gall bladder

Rectum

Anus

Lipase

Duodenum

Pancreatin

trypinsin

Salivary glands

Small intestine epithelium

2) Challenge question, if Billy ate a bunch of Tums (antacids) BEFORE eating at In-n-Out
would his digestion be affected? If so, how? Be sure to describe how the enzymes
would/could be affected and why.
If Billy would of eaten tums prior to eating the burgers his digestion would have been greatly
reduced. Once Billy hate the burgers his digestive system would of gotten to work. The enzymes
would of helped improve his ability to digest, but since the tums were already in his stomach,
this affect would be neutralized. The tums would of stopped the acids in his body from being
able to properly digest food.