METHODOLOGY

To sterilize the carrot seed, the carrot seeds were placed and covered in a small beaker with a
a 15 percent silver nitrate solution.
Since carrot seeds are small , 10 cm3 of solution is adequate to sterilize 20 30 seeds
The seed suspension was swirled for 15 sec and then the mixture was poured quickly through
a funnel lined with a cone of filter paper

The filter was allowed to drain. It was opened. And the seeds was let to air dry for 2-4 hrs.
After the seeds have dried adequately, 20-30 was sprinkled on to a Petri dish containing
standard culture medium (table 1 ).
The edge of the Petri dish was sealed with parafilm and was incubated in the light. The silver
nitrate often darkens the medium right around the seeds. Germination is not affected; it will
commence 4-7 days after the seeds are sown.

Preparation of callus cultures

To initiate a callus culture, sterile forceps was used to medium, and it was placed in
an empty sterile Petri dish.
Leaf, stem, and root tissue were separated with a sterile scalpel. The desired tissue was cut
into sections 1-5 mm long
The cut tissue sections was transferred with sterile forceps, to callus initiation medium (table
1)
4-5 tissue sections was put on each of 2-3 plates. The length and width of all tissue pieces
were recorded.