Alp DRG Rindang 12 Nov 2014.exp 11/13/14-03:12:23 1/14

alp drg Rindang 12 Nov 2014.
exp
11/13/14-03:12:23
1/14
< Experiment title >

Name :
User :
Note :
Date :
11/13/14
Time :
03:12:23
< Protocol parameters >

Experiment file path :
alp drg Rindang 12 Nov 2014.exp
Measurement type :
End point
Measurement mode :
Continuous
Main_1 filter(nm) :
450
Starting method :
Immediate
Need shake :
Yes
Shake speed :
Low
Shake time(sec.) :
Need incubate :
No
Need quantitative :
Yes
Quant. method :
Curve on plate
Quant. standards number :
Quant. replicates number :
Curve fit method :
4 param logistic
Need Cutoff :
No
Need ratio/inhibition :
No
< Plate layout >

1
STD07-1
STD07-2
SAM01-1
SAM02-1
SAM03-1
SAM04-1
...
...
...
STD06-1
STD06-2
SAM05-1
SAM06-1
SAM07-1
SAM08-1
...
...
...
STD05-1
STD05-2
SAM09-1
SAM10-1
SAM11-1
SAM12-1
...
...
...
STD04-1
STD04-2
SAM13-1
SAM14-1
SAM15-1
SAM16-1
...
...
...
STD03-1
STD03-2
SAM17-1
SAM18-1
SAM19-1
SAM20-1
...
...
...
STD02-1
STD02-2
SAM21-1
SAM22-1
SAM23-1
SAM24-1
...
...
...
STD01-1
STD01-2
SAM25-1
SAM26-1
SAM27-1
SAM28-1
...
...
...
BLK01-1
BLK01-2
SAM29-1
SAM30-1
SAM31-1
SAM32-1
...
...
...
0.162
0.213
0.052
0.039
0.039
0.042
...
...
...
0.117
0.129
0.038
0.038
0.038
0.039
...
...
...
< Raw abs. matrix >

P1
11/13/14-03:12:23
2/14
0.074
0.081
0.039
0.039
0.038
0.042
...
...
...
0.051
0.054
0.038
0.039
0.038
0.041
...
...
...
0.043
0.045
0.040
0.039
0.038
0.040
...
...
...
0.043
0.041
0.038
0.038
0.038
0.040
...
...
...
0.046
0.044
0.039
0.038
0.038
0.039
...
...
...
0.043
0.042
0.038
0.038
0.039
0.040
...
...
...
0.119
0.170
0.009
-0.004
-0.004
-0.001
...
...
...
0.074
0.086
-0.005
-0.005
-0.005
-0.004
...
...
...
0.031
0.038
-0.004
-0.004
-0.005
-0.001
...
...
...
0.008
0.011
-0.005
-0.004
-0.005
-0.002
...
...
...
0.000
0.002
-0.003
-0.004
-0.005
-0.003
...
...
...
0.000
-0.002
-0.005
-0.005
-0.005
-0.003
...
...
...
0.003
0.001
-0.004
-0.005
-0.005
-0.004
...
...
...
0.000
0.000
-0.005
-0.005
-0.004
-0.003
...
...
...
< Blanked abs. matrix >

P1
11/13/14-03:12:23
3/14
< Calibration >

Calib. curve
C01
Name
Meas.
STD01
Fit type :
4 param logistic
0.004
Meas. scale :
linear
0.002
Conc. scale :
linear
0.003
C02
STD02
Conc.
3.120
0.001
-0.001
0.000
C03
STD03
Abs.
6.250
0.001
0.003
0.002
C04
STD04
12.500
0.009
0.012
0.011
C05
STD05
25.000
0.032
0.039
0.036
C06
STD06
50.000
0.075
0.087
0.081
C07
STD07
0
100.000
Formula :
0.120
Abs = (a0-a3)/(1+power(C/a2,a1))+a3
0.171
a0 :
0.00003862
a1 :
1.741
a2 :
133.1
a3 :
0.2161
R2 :
0.9991
0.146
200.000
Residuals table
C(set)
Abs.
C(cal)
Residual
C01
3.120
0.003
11.414
8.294
C02
6.250
0.000
6.250
0.000
C03
12.500
0.002
8.985
-3.515
C04
25.000
0.011
23.355
-1.645
C05
50.000
0.036
51.739
1.739
C06
100.000
0.081
99.170
-0.830
C07
200.000
0.146
200.366
0.366
Conc. matrix
11/13/14-03:12:23
4/14
200.366
200.366
23.355
0.000
0.000
0.000
...
...
...
99.170
99.170
0.000
0.000
0.000
0.000
...
...
...
51.739
51.739
0.000
0.000
0.000
0.000
...
...
...
23.355
23.355
0.000
0.000
0.000
0.000
...
...
...
8.985
8.985
0.000
0.000
0.000
0.000
...
...
...
6.250
6.250
0.000
0.000
0.000
0.000
...
...
...
11.414
11.414
0.000
0.000
0.000
0.000
...
...
...
0.000
0.000
0.000
0.000
0.000
0.000
...
...
...
Well
Replicate
Abs.
SD
CV%
Conc.
Unit
A3
0.009
........
........
........
........
........
........
0.009
0.000
23.355
ng/ml
A4
-0.004
........
........
........
........
........
........
-0.004
0.000
0.000
ng/ml
A5
-0.004
........
........
........
........
........
........
-0.004
0.000
0.000
ng/ml
A6
-0.001
........
........
........
........
........
........
-0.001
0.000
0.000
ng/ml
B3
-0.005
........
........
........
........
........
........
-0.005
0.000
0.000
ng/ml
B4
-0.005
........
........
........
........
........
........
-0.005
0.000
0.000
ng/ml
B5
-0.005
........
........
........
........
........
........
-0.005
0.000
0.000
ng/ml
B6
-0.004
........
........
........
........
........
........
-0.004
0.000
0.000
ng/ml
C3
-0.004
........
........
........
........
........
........
-0.004
0.000
0.000
ng/ml
C4
-0.004
........
........
........
........
........
........
-0.004
0.000
0.000
ng/ml
C5
-0.005
........
........
........
........
........
........
-0.005
0.000
0.000
ng/ml
C6
-0.001
........
........
........
........
........
........
-0.001
0.000
0.000
ng/ml
D3
-0.005
........
........
........
........
< Data list >

SAMPLES
Name
SAM01
SAM01_avg
SAM02
SAM02_avg
SAM03
SAM03_avg
SAM04
SAM04_avg
SAM05
SAM05_avg
SAM06
SAM06_avg
SAM07
SAM07_avg
SAM08
SAM08_avg
SAM09
SAM09_avg
SAM10
SAM10_avg
SAM11
SAM11_avg
SAM12
SAM12_avg
SAM13
SAM13_avg
11/13/14-03:12:23
5/14
........
........
-0.005
0.000
0.000
ng/ml
D4
-0.004
........
........
........
........
........
........
-0.004
0.000
0.000
ng/ml
D5
-0.005
........
........
........
........
........
........
-0.005
0.000
0.000
ng/ml
D6
-0.002
........
........
........
........
........
........
-0.002
0.000
0.000
ng/ml
E3
-0.003
........
........
........
........
........
........
-0.003
0.000
0.000
ng/ml
E4
-0.004
........
........
........
........
........
........
-0.004
0.000
0.000
ng/ml
E5
-0.005
........
........
........
........
........
........
-0.005
0.000
0.000
ng/ml
E6
-0.003
........
........
........
........
........
........
-0.003
0.000
0.000
ng/ml
F3
-0.005
........
........
........
........
........
........
-0.005
0.000
0.000
ng/ml
F4
-0.005
........
........
........
........
........
........
-0.005
0.000
0.000
ng/ml
F5
-0.005
........
........
........
........
........
........
-0.005
0.000
0.000
ng/ml
F6
-0.003
........
........
........
........
........
........
-0.003
0.000
0.000
ng/ml
G3
-0.004
........
........
........
........
........
........
-0.004
0.000
0.000
ng/ml
G4
-0.005
........
........
........
........
........
........
-0.005
0.000
0.000
ng/ml
G5
-0.005
........
........
........
........
........
........
-0.005
0.000
0.000
ng/ml
G6
-0.004
........
........
........
........
........
........
-0.004
0.000
0.000
ng/ml
H3
-0.005
........
........
........
........
........
........
-0.005
0.000
0.000
ng/ml
H4
-0.005
........
........
........
........
........
........
-0.005
0.000
0.000
ng/ml
H5
-0.004
........
........
........
........
........
........
-0.004
0.000
0.000
ng/ml
H6
-0.003
........
........
........
........
........
........
-0.003
0.000
0.000
ng/ml
Well
Replicate
Abs.
SD
CV%
Conc.
Unit
STD01
G1
0.003
........
........
........
........
STD01
G2
0.001
........
........
........
........
SAM14
SAM14_avg
SAM15
SAM15_avg
SAM16
SAM16_avg
SAM17
SAM17_avg
SAM18
SAM18_avg
SAM19
SAM19_avg
SAM20
SAM20_avg
SAM21
SAM21_avg
SAM22
SAM22_avg
SAM23
SAM23_avg
SAM24
SAM24_avg
SAM25
SAM25_avg
SAM26
SAM26_avg
SAM27
SAM27_avg
SAM28
SAM28_avg
SAM29
SAM29_avg
SAM30
SAM30_avg
SAM31
SAM31_avg
SAM32
SAM32_avg
STANDARDS
Name
STD01_avg
11/13/14-03:12:23
6/14
........
........
0.002
0.001
50
11.414
ng/ml
STD02
F1
0.000
........
........
........
........
STD02
F2
-0.002
........
........
........
........
........
........
-0.001
0.001
-100
6.250
ng/ml
STD03
E1
0.000
........
........
........
........
STD03
E2
0.002
........
........
........
........
........
........
0.001
0.001
100
8.985
ng/ml
STD04
D1
0.008
........
........
........
........
STD04
D2
0.011
........
........
........
........
........
........
0.010
0.002
15.79
23.355
ng/ml
STD05
C1
0.031
........
........
........
........
STD05
C2
0.038
........
........
........
........
........
........
0.035
0.004
10.14
51.739
ng/ml
STD06
B1
0.074
........
........
........
........
STD06
B2
0.086
........
........
........
........
........
........
0.080
0.006
7.5
99.170
ng/ml
STD07
A1
0.119
........
........
........
........
STD07
A2
0.170
........
........
........
........
........
........
0.145
0.026
17.65
200.366
ng/ml
STD02_avg
STD03_avg
STD04_avg
STD05_avg
STD06_avg
STD07_avg
< End >
10
11
12
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
10
11
12
...
...
...
...
...
...
11/13/14-03:12:23
7/14
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
10
11
12
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
11/13/14-03:12:23
8/14
4 param logistic
(C/a2,a1))+a3
210
Conc.
11/13/14-03:12:23
9/14
10
11
12
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
...
Cutoff
R/I(%)
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
11/13/14-03:12:23
10/14
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
N/A
........
N/A
N/A
Cutoff
R/I(%)
N/A
........
N/A
........
11/13/14-03:12:23
11/14
N/A
N/A
N/A
........
N/A
........
N/A
N/A
N/A
........
N/A
........
N/A
N/A
N/A
........
N/A
........
N/A
N/A
N/A
........
N/A
........
N/A
N/A
N/A
........
N/A
........
N/A
N/A
N/A
........
N/A
........
N/A
N/A
11/13/14-03:12:23
12/14
s1
s2
s3
s4
s5
s6
K3
p3
k7
p7
k14
p14
k21
p21
0.162
0.213
0.117
0.129
0.074
0.081
0.051
0.054
0.043
0.045
0.043
0.041
0.052
0.038
0.039
0.038
0.040
0.038
0.039
0.038
20.37867
6.912492
7.843253
6.912492
8.7788
6.912492
7.843253
6.912492
mean
conc (ng/mL)
0.188
200
0.123
100
0.078
50
0.053
25
0.044
12.5
0.042
6.25
0.039
0.038
0.039
0.039
0.039
0.038
0.038
0.038
7.843253
6.912492
7.843253
7.843253
7.843253
6.912492
6.912492
6.912492
0.039
0.038
0.038
0.038
0.038
0.038
0.038
0.039
7.843253
6.912492
6.912492
6.912492
6.912492
6.912492
6.912492
7.843253
250
200
150
f(x) = 2393.7797913905x^2 + 746.5312109563x - 24.917163079

R = 0.9980334757
Column F
100
Polynomial (Column F)
50
0
0.000
0.042
0.039
0.042
0.041
0.040
0.040
0.039
0.040
10.66425
7.843253
10.66425
9.719133
8.7788
8.7788
7.843253
8.7788
0.050
0.100
0.150
0.200

Alp DRG Rindang 12 Nov 2014.exp 11/13/14-03:12:23 1/14

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Alp DRG Rindang 12 Nov 2014.exp 11/13/14-03:12:23 1/14

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Formati disponibili

alp drg Rindang 12 Nov 2014.

< Experiment title >

< Protocol parameters >

alp drg Rindang 12 Nov 2014.exp

Quant. standards number :

Quant. replicates number :

Curve fit method :

< Plate layout >

< Raw abs. matrix >

alp drg Rindang 12 Nov 2014.exp

< Blanked abs. matrix >

alp drg Rindang 12 Nov 2014.exp

< Calibration >

alp drg Rindang 12 Nov 2014.exp

< Data list >

alp drg Rindang 12 Nov 2014.exp

alp drg Rindang 12 Nov 2014.exp

< End >

alp drg Rindang 12 Nov 2014.exp

alp drg Rindang 12 Nov 2014.exp

alp drg Rindang 12 Nov 2014.exp

alp drg Rindang 12 Nov 2014.exp

alp drg Rindang 12 Nov 2014.exp

alp drg Rindang 12 Nov 2014.exp

f(x) = 2393.7797913905x^2 + 746.5312109563x - 24.917163079

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