Pristane-Induced Arthritis (PIA) in rats

Model description
Pristane-induced arthritis (PIA) is one of the most
reproducible forms of experimental arthritis in rats.
Characteristics
PIA is the preclinical model of arthritis that mimics most
of the criteria used for diagnosis of the human RA. Hence,
PIA displays symmetry of inflammation with a chronic
relapsing disease course and infiltration of T cells,
infl ammation, edema and erosive destruction of
peripheral joints.
PIA is the most predictable model of arthritis in rats with
incidence above 95%. PIA is dependent on CD4+ T cells
with possibility of adoptive transfer of disease. In
comparison to collagen-induced arthritis (CIA) in rats, PIA
has an earlier onset, is less variable and have an
inflammation that is more localised to wrist/ankle joints.
Experimental outline
Induction
Duration
Onset
Peak of disease
Strain
Positive Controls

subcutaneous injection with pristane

25-30 days
day 8-12
day 15-20 after induction
DA (other strains possible)
Methotrexate, Dexamethasone,
Cyclosporine or Etanercept

Evaluation and output

Clinical signs
General health
Blood analyses

Immunology
Joints

Macroscopic scoring
Body weight and health status
Acute phase proteins (AGP,
CRP), cartilage destruction (COMP),
cytokines and IgG responses
Flow cytometry analysis of cell
composition and markers
ImmunoHistochemistry

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Read more
- Arthritis induced in rats with nonimmunogenic adjuvants as models
for rheumatoid arthritis. Holmdahl et al. Immunol Rev. 2001
- A systematic comparison between collagen-induced arthritis and
pristane induced arthritis in Dark Agouti rats. Hou et al. Clin Exp
Rheumatol. 2010
2012 Redoxis AB