Directions:

Ive posted data from sample experiments on different

factors affecting catalase activity. You may use this data if
you are not content with your own, but you must make
note of this on your lab handout.
For each factor, Ive posted TWO different options of data
sets to choose from. I encourage you to use the links to
read about each experiment and to determine how
results were obtained.
Keep in mind that many of these tables would NOT
receive full points due to incorrect headings, number of
decimal points, etc. It is your responsibility to fix these
before recording the data on your sheet.

Factor: Substrate concentration (percentage of

hydrogen peroxide)

Source: http://luno2012.hubpages.com/hub/A-level-Biology-Coursework-enzyme-catalase

http://s1.hubimg.com/u/6541430_f520.jpg

Factor: Temperature

Source: http://www.all-science-fair-projects.com/print_project_1325_105

Source: http://www.all-science-fair-projects.com/print_project_1326_105

Factor: pH
Volume of
catalase (cm3)

pH

Trial 1 - time
Trial 2 - time
taken for the
taken for the
gas to reach
gas to reach 10
10cm3 (s)
cm3 (s)
5
4.4
16.93
18.85
5
5.2
14.63
16.89
5
6.5
10.5
12.28
5
7.5
9.45
10.62
5
8.4
6.45
8.92
Source: http://www.123helpme.com/view.asp?id=147741

pH Level

Control (7)

Trial 3 - time
taken for the
gas to reach 10
cm3 (s)
15.82
15.67
14.66
8.86
7.25

Average

17.2
15.73
12.48
9.64
7.54

Reaction Rate (ml/minute) 1.5 ml/min 1.3 ml/min 1 ml/min .55 ml/min
Table 2: The reaction rate for each pH level.

Source:
https://docs.google.com/a/asu.edu/document/d/1qe_ftD0j107KictA6gqRoXD9hAVzdAVap59f1PFxWj4/edit