Fig 8-12

cells grown
in 15N

cells grown
in 14N

Fig 8-21
hydroxyl group at 3
end of growing chain

deoxynucleotide
triphosphate

Fig 8-21
hydroxyl group at 3
end of growing chain

deoxynucleotide
triphosphate

Fig 8-21

new
phosphodiester
bond
pyrophosphate
(PPi)

this becomes new 3 end

G
A

C
A

A
G

G
T

G
A

C
A

A
G

G
T

DNA polymerase (5'

(3' ->
-> 3'
5' polymerase)
exonuclease)

RNA primer
OH

5'

OH

OH

U
G
A

3'

C
A
C

G
G

OH

C
C

oops!

G
C

G
T

5'

DNA polymerase (5' -> 3' exonuclease)

new DNA

RNA primer
A

G
A

G
C

C
A

A
G

G
T

Fig 8-20

Replication Fork