Biochemical Test for Microbial Identification

Test/ reaction
Catalase
decomposition
Oxidase
oxidation

Substrate *
3% H2O2

Enzyme
Catalase

End-product
H2O

Indicator
H2O2

Pos & Neg

+ gas gas

1% tetramethyl-pphenylenediamine
dihydrochloride
tryptophan

Cytochrome
oxidase

indophenol

+ Dark purple
- Nochange

tryptophanase

indole

Erlichs
+ Ether
cinnamaldehyde

Urease
hydrolysis
PYR
hydrolysis
Hippurate
Hydrolysis

urea

urease

ammonia

phenol red

L-pyrrolidonyl-Bnaphthylamide
hippuric acid

L-pyrroglutamylaminopeptidase
hippurase

Bnaphthylamimide
glycine,
benzoic acid

N,N,methylamino
cinnamaldehyde
ninhydrin

Acetamide
Utilzn,deamination
Acetate
Utilization#
Bile
Esculin hydrolysis

Acetamide

deaminase

ammonia

bromthymol blue

Sodium acetate

Source of carbon

Shift to alkaline

Bromthymol blue

40 %Bile-inhibtor
esculin

hydrolase

esculetin

Ferric amm. citrate

+ red
- yellow
+bl green
-nochange
+ bright red
- no change
+ red
- orange
+purple
-yellow
or pink
+ blue
- green
+ blue
- green
+ dark brown
- light brown

Bile solubility

Bile ,
sod.desoxychollate*
Blood*

Autolytic enzyme

Lyses of
membrane
Lysis of rbc

Not inhibited

alkaline

Bromthymol blue

Clumping factor
Clotting factor
Amine
Alkaline
Hydrolyze DNA

* slide
* tube
Bromcresol purple

Liquid gelatine

Indole
utilization

CAMP test
lysis
Cetrimide inhibition

CAMP Factor
Beta lysin

Cetrimide*

Citrate
utilization
Coagulase

Sodium citrate

Decarboxylase
or hydrolyse
DNA hydrolysis
depolymerization

lysine
DNA

Source of carbon
& inorganic salt
Boundcoagulase
Free coagulase
Decarboxylase or
deaminate
DNAse

Gelatin hydrolysis

Gelatine*

gelatinase

Methyl Red

glucose

Acid 4.5 pH

Methyl red

Voges Proskauer

Glucose

Acetoin Neutral

Nitrate reduction

Nitrate

nitrite

Alpha napthol
40% KOH
Sulfanilic acid
Alpha
naphthylamine

Prepared by:

Citrated plasma*

Prof. Rogelio S. Cruz

CEU, College of Med. Tech.
Mendiola, Manila

DNA-methyl green
Toluidine blue

+ clear
- turbid
+arrowhead
- absent
+ growth
- absent
+ blue
- green
+cellclumping
+ clot
+ purple
- yellow
+ fading clear
zone
+ clear zone
+ liquid 4oC
- solid 4oC
+ red
-orange-yelw

+ red
- yellow
+ red
- yellow