Sei sulla pagina 1di 30

Project Connective Tissue

White Adipose CT
Slides 2-3: unstained slide
Slides 4-5: stained slide (lipid droplet intact)
Author: Lindsay Estes
White adipose tissue (loose ct): stores energy in the form of lipids (adipocytes),
provides cushion and insulation to the body, and produces a number of hormones.
Nuclei
Capillary
Fat vacuoles
Fat vacuoles
A
B C
White adipose tissue (loose ct): stores energy in the form of lipids (adipocytes),
provides cushion and insulation to the body, and produces a number of hormones.
Fat droplets
Fat droplets
A
B C
Lacunae
Chondrocyte
Territorial
matrix
Perichondrium
Chondroblast
Fibroblast
Matrix
Yohannes Kidane
09/16.2014; Hyaline cartilage; Leica DM 500 # 3;
091614YK18
Mag: 40x; Ex: 134ms; Gain: 1.0x; Sa: 1.0; Gamma: 1.0
Yohannes Kidane
A
C
B
D
Fluorescence channel images: Hyaline Cartilage
A. Green B. Red C. Blue D. Overlay
Fibroblast
Dense regular fibrous
connective tissue
Collagen matrix
Yohannes Kidane
09/16.2014; Mammal tendon Leica DM 500 # 3; 092014YK24
Mag: 100x; Ex: 454.2ms; Gain: 1.0x; Sa: 1.0; Gamma: 1.0
Yohannes Kidane
Fluorescence channel images: Mammal tendon
A. Brightfield B. Green C. Red D. UV
A
C
B
D
Brown Adipose tissue. 40x Brightfield 35.9ms Leica
Brown adipose is found in neonatal humans and in animals that
hibernate. Fat droplets are smaller compared to those in white
adipose tissue and have numerous mitochondria. In hibernating
animals, the uncoupling of oxidation from phosphorylation
realeases heat rather than producing HTP.

Christie Ward
Brn Adipose UV 1.3 sec Leica Brn Adipose Blue light 1.2 sec Leica
Brn Adipose Green light 1.5 sec Leica Brn Adipose RGB overlay using Image J

Christie Ward
ENDOCHONDRAL OSSIFICATION
CHRONDROCYT
ES
LACUNAE
ZONE OF
ENDOCHONDRA
L
OSSIFICATION
FUNCTION: Endochondral ossification is one of the two essential processes during fetal
development by which bone tissue is created. Cartilage is present during endochondral
ossification. Essential process during rudimentary formation of long bones, the growth of the length
of long bones, and the natural healing of bone fractures.
MATRIX




Sam Rodriguez
Ground Bone
Ground Bone
Ground Bone
Developing Membranous Bone
Connective
tissues
Angel mcgee
9/16/2014


Aorta
AORTA
1.9 MS
LOW GAIN
Sm- Smooth Muscle
Act- Areolar Connective
Tissue
Ef- Elastin Fibers
The ascending aorta begins at the
opening of the aortic valve at the
heart



EF
SM
ACT
Elastic cartilage
Elastic cartilage
3.5 ms
Low Gain
M- Matrix
Cc- Chondrocyte
Pd- Perichondrim
Ef- Elastin Fibers
L- Lacunae

Elastic cartilage is found in the
ear and larynx

EF
CC
PD
M
L
Areolar connective tissue
collagen
Fibroblasts
Human scalp [skin cornified human sec Wards 93 W 7038]
Dense fibrous connective tissue, the irregular form
L
.

T
i
p
l
e
r

U
b
b
e
l
o
h
d
e

Dense irregular tissues have fibroblasts and
matrix fibers (mostly collagen) that are oriented
differently, e.g. not oriented in a parallel fashion.
This tissue is present in the skin.
FL Composite
FL UV channel
FL Red channel
FL Green channel
L
.

T
i
p
l
e
r

U
b
b
e
l
o
h
d
e


&


Y
o
h
a
n
n
e
s

K
i
d
a
n
e

King Burddet Jr.
King Burddet Jr. 09/16/14; Zeiss Axioimager; Mammal Elastic Cartilage
KBMCartilageElastic16Sep14; Mag: 20x; Exposure: 963ms; Gain: 1.0x; Saturation: 0.0
Chondrocyte
Lacunae
Territorial
Matrix
Matrix
King Burddet Jr.
King Burddet Jr. 09/16/14; Zeiss Axioimager; Mammal Elastic Cartilage
KBMCartilageElastic16Sep14; Mag: 20x; Exposure: 516ms; Gain: 1.0x; Saturation: 0.0
Fluorescence channel images: Elastic Cartilage
A. GFP B. Cy3 C. DAPI D. Overlay
King Burddet Jr.
A
C
B
D
Matrix
Collagen
Elastin
Fibroblasts
LOOSE AREOLAR TISSUE
LAURI ZAKER
The function of loose areolar tissue is to hold organs
in place
And attaches epithelia to underlying tissues. It also
allows
For diffusion of nutrients to epithelial cells.
LOOSE AREOLAR TISSUE
Plasma Cell
LAURI ZAKER
Macrophages
LOOSE AREOLAR TISSUE
LAURI ZAKER
Mast cell
LAURI ZAKER
Loose Areolar Tissue
Red Fluorescent
Green Fluorescent
Blue Fluorescent
RGB Overlay
LAURI ZAKER