Slide #1

6850/6890 Splitless Animation -

Split/Splitless Inlet - Component Identification
Septum
Liner
Liner O-Ring
Gold Seal
Capillary Column
9/27/02
Slide #2
6850/90 Split/Splitless Inlet - Component Identification
Septum
SEPTUM PURGE
VENT
Split Vent
Slide #3
6850/90 Splitless Animation - Default Conditions
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
PURGE
VALVE
OPEN
TOTAL FLOW
CONTROL LOOP
SPLIT
VENT
GOLD SEAL
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
0

ML/MIN
SPLIT VENT
TRAP
0

PSI
Slide #4
6850/90 Splitless Animation - Total Flow Setting
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
PURGE
VALVE
OPEN
TOTAL FLOW
CONTROL LOOP
SPLIT
VENT

55 ML/MIN
GOLD SEAL
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
PRIMARY FLOW PATH
REDUCED FLOW PATH
55

ML/MIN
SPLIT VENT
TRAP
0

PSI
Slide #5
6850/90 Splitless Animation - Inlet Pressure Setting
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
OPEN
TOTAL FLOW
CONTROL LOOP
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
PRIMARY FLOW PATH
REDUCED FLOW PATH
55

ML/MIN
SPLIT VENT
TRAP
10

PSI
SPLIT
VENT

50 ML/MIN
COLUMN FLOW
2 ML/MIN
PRESSURIZED GAS
BACK PRESSURE CONTROL LOOP
Mode: Splitless
Temp 250 250
Pressure 10.0 10.0
Purge Time 0.50
Purge Flow 50.0
Tot Flow 55.0
Gas Saver OFF
Slide #6
6890 Splitless Animation - GC Status before Pre-Run
Slide #7
6890 Splitless Animation - GC Status after Pre-Run
Slide #8
6850/90 Splitless Animation - Injection Prep - Run
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
CLOSED
FORWARD PRESSURE
CONTROL LOOP
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
PRIMARY FLOW PATH
REDUCED FLOW PATH
5

ML/MIN
SPLIT VENT
TRAP
10

PSI
SPLIT
VENT

0 ML/MIN
COLUMN FLOW
2 ML/MIN
PRESSURIZED GAS
Mode: Splitless
Temp 250 250
Pressure 10.0 10.0
Purge Time 0.50
Purge Flow 50.0
Tot Flow 5.0
Gas Saver OFF
Slide #9
6850/90 Splitless Prep Run - Ready
Animation
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
CLOSED
FORWARD PRESSURE
CONTROL LOOP
GOLD SEAL
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
PRIMARY FLOW PATH
REDUCED FLOW PATH
5

ML/MIN
SPLIT VENT
TRAP
10

PSI
SPLIT
VENT

0 ML/MIN
COLUMN FLOW
2 ML/MIN
PRESSURIZED GAS
Slide #10
6850/90 Splitless Begin Injection
Animation
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
CLOSED
FORWARD PRESSURE
CONTROL LOOP
GOLD SEAL
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
5

ML/MIN
SPLIT VENT
TRAP
10

PSI
SPLIT
VENT

0 ML/MIN
COLUMN FLOW
2 ML/MIN
Slide #11
6850/90 Splitless Inject Sample
Animation
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
CLOSED
FORWARD PRESSURE
CONTROL LOOP
GOLD SEAL
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
5

ML/MIN
SPLIT VENT
TRAP
10

PSI
SPLIT
VENT

0 ML/MIN
COLUMN FLOW
2 ML/MIN
Slide #12
6850/90 Splitless Sample Vaporizes
Animation
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
CLOSED
FORWARD PRESSURE
CONTROL LOOP
GOLD SEAL
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
5

ML/MIN
SPLIT VENT
TRAP
10

PSI
SPLIT
VENT

0 ML/MIN
COLUMN FLOW
2 ML/MIN
Slide #13
6850/90 Splitless Liner Capacity?
Animation
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
CLOSED
FORWARD PRESSURE
CONTROL LOOP
GOLD SEAL
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
5

ML/MIN
SPLIT VENT
TRAP
10

PSI
SPLIT
VENT

0 ML/MIN
COLUMN FLOW
2 ML/MIN
Larger O.D Liner
to minimize sample
contact with inlet body
Tapered Liner to
minimize sample contact
with Gold Seal
Slide #14
6850/90 Splitless Sample Transfer to
Animation Column - Liner Volume
vs. Column Flow
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
CLOSED
FORWARD PRESSURE
CONTROL LOOP
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
5

ML/MIN
SPLIT VENT
TRAP
10

PSI
SPLIT
VENT

0 ML/MIN
COLUMN FLOW
2 ML/MIN
OVEN < BP OF SOLVENT
SAMPLE RE-CONDENSES
AT HEAD OF COLUMN
FOR 1 ML LINER VOLUME
AND 2 ML/MIN COLUMN FLOW
PURGE DELAY SHOULD BE
0.5 MINUTES MINIMUM
Slide #15
6850/90 Splitless Pulsed Splitless Mode
- Animation Reduced Purge Time
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
CLOSED
FORWARD PRESSURE
CONTROL LOOP
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
7

ML/MIN
SPLIT VENT
TRAP
20

PSI
SPLIT
VENT

0 ML/MIN
OVEN < BP OF SOLVENT
SAMPLE RE-CONDENSES
AT HEAD OF COLUMN
Mode: Pulsed Splitless
Temp 250 250
Pressure 10.0 10.0
Pulse Press 20.0
Pulse Time 0.25
Purge Time 0.25
Purge Flow 50.0
Tot Flow 7.0
Gas Saver OFF
COLUMN FLOW INCREASES TO
4 ML/MIN CUTTTING PURGE TIME IN HALF
Slide #16
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
CLOSED
FORWARD PRESSURE
CONTROL LOOP
GOLD SEAL
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
5

ML/MIN
SPLIT VENT
TRAP
10

PSI
SPLIT
VENT

0 ML/MIN
COLUMN FLOW
2 ML/MIN
OVEN < BP OF SOLVENT
SAMPLE RE-CONDENSES
AT HEAD OF COLUMN
6850/90 Splitless Sample Re-Condenses
Animation At the Head of the Column

Slide #17
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
CLOSED
FORWARD PRESSURE
CONTROL LOOP
GOLD SEAL
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
5

ML/MIN
SPLIT VENT
TRAP
10

PSI
SPLIT
VENT

0 ML/MIN
COLUMN FLOW
2 ML/MIN
OVEN < BP OF SOLVENT
SAMPLE RE-CONDENSES
AT HEAD OF COLUMN
6850/90 Splitless Sample Transfer to
Animation Column Continues...
Slide #18
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
CLOSED
FORWARD PRESSURE
CONTROL LOOP
GOLD SEAL
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
5

ML/MIN
SPLIT VENT
TRAP
10

PSI
SPLIT
VENT

0 ML/MIN
COLUMN FLOW
2 ML/MIN
OVEN < BP OF SOLVENT
SAMPLE RE-CONDENSES
AT HEAD OF COLUMN
6850/9090 Splitless Until the end of the
Animation Splitless Purge Delay...
Slide #19
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
OPEN
TOTAL FLOW
CONTROL LOOP
GOLD SEAL
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
PRIMARY FLOW PATH
REDUCED FLOW PATH
55

ML/MIN
SPLIT VENT
TRAP
10

PSI
SPLIT
VENT

50 ML/MIN
COLUMN FLOW
2 ML/MIN
PRESSURIZED GAS
BACK PRESSURE CONTROL LOOP
6850/90 Splitless Animation - Inlet is Returned to Split
Configuration - Residual Solvent is flushed from Inlet -
GC Temperature Program and Separation Begins
Slide #20
6850/90 Splitless Press Fit Splitless Liner -
Animation With Hole - No sample
Contact with gold seal
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
CLOSED
FORWARD PRESSURE
CONTROL LOOP
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
7

ML/MIN
SPLIT VENT
TRAP
20

PSI
SPLIT
VENT

0 ML/MIN
OVEN < BP OF SOLVENT
SAMPLE RE-CONDENSES
AT HEAD OF COLUMN
Mode: Pulsed Splitless
Temp 250 250
Pressure 10.0 10.0
Pulse Press 20.0
Pulse Time 0.25
Purge Time 0.25
Purge Flow 50.0
Tot Flow 7.0
Gas Saver OFF
COLUMN FLOW INCREASES TO
4 ML/MIN CUTTTING PURGE TIME IN HALF
Slide #21
FLOW
SENSOR
PROPORTIONAL
VALVE 1
PRESSURE
SENSOR
SEPTUM PURGE
REGULATOR
FIXED @ 3ML/MIN
PURGE
VALVE
OPEN
TOTAL FLOW
CONTROL LOOP
GOLD SEAL
INPUT
PRESSURE
PROPORTIONAL
VALVE 2
80 PSI
PRIMARY FLOW PATH
REDUCED FLOW PATH
55

ML/MIN
SPLIT VENT
TRAP
10

PSI
SPLIT
VENT

50 ML/MIN
COLUMN FLOW
2 ML/MIN
PRESSURIZED GAS
BACK PRESSURE CONTROL LOOP
6850/90 Splitless Animation - Inlet is Returned to Split
Configuration - Residual Solvent is flushed from Inlet -
through the hole in the liner..
Slide #22
Splitless Liner Selections
Single
Taper
with wool
Double
Taper
Single
Taper
Double
Taper
Single
Taper
NO wool
Direct Connect
Hole
for
EPC
Slide #23
6890 Splitless Chromatogram -
min
0 1 2 3 4 5 6 7 8 9
pA
0
500
1000
1500
2000
FID1 A, (A:\FIDSPLS.D)
Area: 1.41189e+007

1
.
0
0
1

5
.
9
3
7

6
.
7
9
5

7
.
6
3
7