RAJALAKSHMI ENGINEERING COLLEGE, THANDALAM, CHENNAI.

DEPARTMENT OF BIOTECHNOLOGY
INSTRUMENTAL METHODS OF ANALYSIS QUESTION BANK
PART – A
1. Name the different types of analysis.
2. Define accuracy
3. Write short notes on precision
4. What is sensitivity?
5. Write short notes on error and its types
6. Explain selectivity
7. Write short notes on the following
I). Detector, II).data domain, III). Sensor
8. Write short notes on Raleigh scattering.
9. What is atomization?
10. What is nephlometry and turbidimetry?
11. Why low concentrated suspensions are analysed by nephlometry whereas high
concentrated ones by turbidimetry?
12. What is tyndal effect?
13. Which of the following can be used as nephlometer.how?
a).flourimeter, b).colorimeter.
14. Compare and contrast of nephlometry and flourimetry

15. What are radio isotopes?

16. Discuss the role of radio isotopes in biological system.
17. What is wavelength? How is it expressed (unit)?
18. What is frequency? How is it expressed (unit)?
19. What changes in the molecule occur when the following is passed:
20. Gamma rays, b).microwaves). Radiofrequency waves
21. Write short notes on the following:
a).λ max, b).Emax, c) EMR
22. What is a transducer in an analytical instrument?
23. Define the term effective bandwidth of a filter?
24. What is resolution?
25. What is the principle involved in Fourier transform measurements?
26. What is band pass?
27. What is a filter? Name the different types?
28. What is beer Lambert’s law?
29. What is the principle involved in the UV Spectro photometer?
30. What are the differences between single beam and double beam instruments?
31. If λ is changed, what will happen to A & E?
32. Name the reasons for deviation from beer’s law?
33. What is the principle involved in Raman spectroscopy?
34. What is the principle involved in Auger electron spectroscopy?
35. What is spin quantum number?
36. What is the difference between NMR & PMR?
37. What is shielding and de-shielding?
 38. What is dipole movement?
39. What is FT-IR?
40. How will you identify if the impurities in a drug by IR?
41. How is chemical shift measured?
42. What are the applications of AAS?
43. What is internal reference (or) reference standard?

44. What are the applications of thermo gravitometric methods? What information
can one have from the pyrolysis curves? Describe the essential features of the
chevenard thermobalance.
45. Explain briefly the principle and its applications of the following
46. Differential thermal analysis (ii) enthalpy titrations
47. What are the various thermometric methods of analysis? How are they useful in
analytical chemistry?
48. What is thermo gravimetric analysis?
49. What do you understand by differential thermal analysis? How is DTA used in the
following measurements?
50. a).specific heat, b).heat of reaction.
51. What are the thermogravimetrics titrations? Discuss some important thermometric
titrations.
52. Write short notes on heat of reaction

53. Write short notes on X-ray properties

54. Give an account on X-ray and their properties
55. Name the different types of X-ray detector
56. What is wavelength and wavenunber? How is it expressed (unit)?
57. What is X-ray diffraction (XRD)?
58. Drive Bragg’s law
59. What are the analytical applications of X-ray fluorescence
60. What are advantages of vacuum in X-ray tube?
61. Name the different components of X-ray tube?
62. Explain scintillation and types

PART – B

Explain the various instrumental methods of analysis.

Write a detail account on the following
i).Raleigh scattering, ii).flame atomization, iii).scattering of gases.
2. What is error? Explain the types and their significance.
3. Explain the various methods of calibration
4. Draw a neat sketch on nephlo-turbidimeter and explain the devices of nephlo-
turbidimeter.
5. How can you do quantitative analysis using nephlo-turbidimeter?

6. Write a note on detector and its types?

7. Write a detail account on the following:

 a. (i) Filters (ii) monochromators
8. Describe Fourier transformer measurements? and its applications.
9. Write a short note on the following:
i. Radiation sources (ii) sample container (iii) fibre optics
10. With the help of neat labelled diagram describe a U.V spectrometer.
11. Draw a diagram of Raman spectrometer and explain its working principle.
12. Describe the principle, apparatus system of AAS.
13. Draw a diagram of NMR and explain its working principle& applications.
14. What are the laws governing the adsorption of radiation & its deviations?
15. Write a detail account on the following
i. Isobestic point (ii) vibrations (ii) chemical shift

16. Discuss in a detail account of thermo gravimetric methods and its applications.
17. Draw a diagram of DTA and explain its working principle and applications.
18. Describe the working principle, apparatus and applications of DSC

19. Explain the production of X-rays and their applications

20. Write short notes on the following
i. photographic film,
ii. Photostimulable phosphors,
iii. Ionization chamber,
iv. Semiconductor.
21. Discuss in detail account on absorption of X-rays.
22. What is the scintillation and G-M counter? Explain the types and their
significance.
23. Write short notes on the following:
i. X-ray sources,
ii. X-ray fluorescence
24. Discuss in detail account on X-ray diffraction