Enzyme lab

Effect of substrate concentration on urease

Members
Siraphat Worathanawong 10-7 (Mink)
Watcharit Athikhamanon 10-7 (Key)
Nathadol Jomsiriwattana 10-7 (Nol)
Kantaphon Witjitrasup 10-7 (Tiger)
Pasit Suksure 10-7 (Un)
Saharat Kaewphong 10-7 (Cheak)
Pateepan Sakulratanacharoen 10-7 (Book)
I ntroduction

In this experiment we tried to look how pH can control the color of indicator. We also looked at the effect of
urease from soya beans on urea. For the last experiment we were focus on how substrate's concentrations affect the
enzyme's work.
In procedure 1, we predicted that acid would change to red, base changed to purple and the neutral would
have no change. From procedure 2, the test tube which has both water and enzyme the color will not change, and
for the one that have urea and urease the color will change. And the one that we had choose, which is substrate
concentration we predicted that the one that have the most concentration can change the color easier than others.
Procedure I


Substance

Color


Citric


Red

Sodium bicarbonate


Purple

Distilled water

Blue
Result
Procedure I
Questions

Why is it important to have three reference tubes?
To check if the indicator can work with every states, acid, base and neutral.

Can you predict what color of the solution will be if the urease enzyme convert urea to ammonia?
Blue or purple because ammonia is base.

Procedure 2


Substance


Color


Urea


Light purple


Urea +Enzyme


Purple

Water +Enzyme


Blue
Result
Procedure 2
Questions


What are the purposes of procedure 2 ?
To check if the enzyme is working or not. If the enzyme works probably, the tube which has both urea
and enzyme must change the color to purple.

Why do we need to add an enzyme into a test tube without urea even though we can predict
that no enzyme activity will occur ?
Because we need to make sure that enzyme is neutral. If this test tube change color then it mean that
enzyme is not neutral and urea is not relate with it.

Effect of substrate concentration on urease

Substance

Color

Color (after 10 min)

Smell

5% Urea +Enzyme


Blue

Green

Best

10% Urea +Enzyme


Blue

Green

Normal

20% Urea +Enzyme


Blue

Green

Worst
Result
10 minutes
Effect of substrate concentration on urease
Questions
How does substrate concentration affect the urease activity? How do you know?
The one which has the high concentration will has high rate, take less time, to change into ammonia because from our
experiment the one which has highest concentration has the most smelly.

Why do you think the color of the indicator slowly changes as times passes?
Because it take times to make urea react with water and produce ammonia which is base.

If the concentration of the enzyme was to be increased to twice the amount that was used, what do you think will happen
to the color change relative to time? Explain possible reasons to support your answer.
The color would change faster because the rate of changing urea to ammonia increases but it will faster until some point
which is the maximum point that there are not enough urea to change to ammonia.

Conclusion
From all the result, we can conclude that both indicator and enzyme work pretty well. We
also conclude that enzymes concentration affect the rate of reaction by looking the result that
20% urea has the worst smell when compare with 10% and 5% then we can conclude that 20%
has the most number of ammonia then we can conclude that 20% has the highest rate of
reaction. So we can conclude that .

Thank you for watching!!