Nutrition 310 BCMB 10 HomeworkNo2 Name: \(\) ‘UL (Y\\\A/ XCEPTIONS! 1. Write your mame on al pages @ points) 2. Write Your answers in an organized and neat format, Answers not showing all ‘What i the corresponding mRNA sequence and the aminoacid sequence? (10foint) “Mote cre label 3” ana ond? WRN SCCUGHUOR GL GuGUAS nnd acid N-Fr9-Gly -Gin-Cys -Wal-C (©) Indicate the roe of the following protcinvenzymes in DNA replication in E colt 2 points) ) COSA es rs CORBIN ca wrwDRd c\etuie om ng by bird 8 Garacer fuses aoyncomy be Helicase: pn 6 DNA silt in (SSB) prot (©) Whats the difrence in composition between the CORE enzyme and the HOLO enzyme of E.Coli RNA Polymerase? Hove does thio difference relat tothe functional role ofthe enzyme? points)» name: \WWU) WALA (@) Ardy Kounberg isolated and characterize ie fst DNA polyierne Gu E, cold ‘ctr. A few years aftr this work on DNA polymerase a mutant frm of EE eoll was discovered that had the following charactriaics ‘©The muram bacteria had only 19% of the DNA polymerase J atvity found in wildtype E-coffclls however, cll division occured at the sume rate as in normal : col xls ‘= Temutant £ col cells dying after iadiaton (exposure wo U.Y) ‘Explain the above observations. What do these findings suggest aboot the possible Faction of DNA polymerase in E cli ells? (points) nove fs nivaggd PNP weecruse, i DN poll +O Hx Uae radiottoY : LY Make «comparative lst of difereues between the 3 types of DNA separ systems in E.Coli List the enzymes/protcins involved in cash typeof repair and the typeof damage it cores. (i pons) o3. Glucose and Lactose levels conto te inition Of tanseripon Tae operon of Colt vu ‘hough ther efeets on the laciose represor protein and CAP. Using purdal structure ofthe lac operon, explain whether the operon will b on or aff end the folowing conditions aad azine sre dees the lac operon nd pve your esos 0 Bak or newer) pee er airNAME: Vv 4. Prot the electophoretic pattems that would be obiined when oligonucleotide v [AATCTACGTGCGCA‘T is subjeccd tothe Sanger’s method of DNA sequencing. "The prime that needed for this reaction i composed of CTT. (Show all the fxgments ‘that woul be generated Pshould have 13, as well asthe postion onthe electrophoretic gel. NO need to include the primer) (15 poins) S;GAATCTACGTGCGCAT crs