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Temperature :
Nitrifying bacteria are temperature sensitive. Rapid changes in temperature do not produce rapid changes in growth rates. A slow adaptation period, with a lower than expected rate follows such changes.
Inhibiting Substances : When exposed to more than one inhibitor, the extent of
inhibition increases greatly.
Water level : Water level has a strong effect on the growth rate
of nitrifying bacteria.Optimum water level needed and too high or too low water level may decreasing nitrification process.