Mass Balance of Soaking in Aqueous Ammonia/SSF

Ammonia recycling Biomass 100 lb (dry basis) G:36.1 lb X: 21.4 lb O: 7.8 lb L:17.2 lb
Ammonia

SSF
Treated stover

ARP Reactor
Liquid

Washing
G: 36.1 lb X:19.1 lb

Hydrolysis
Hydrolyzate

Glucose 34.1 lb Soluble sugar Xylose 17.6 lb X: 2.3 lb Residual Sugar Fermentation 24.2 lb Lignin 9.3 lb Others 18.4 lb Residual solid 18.0 lb Lignin 7.9 lb Ethanol 24.3 lb Conversion Efficiency: 85.0% glucan & 72.4% xylan conversion at 15 FPU/g of glucan Measured yield from glucan : 2.44 gallons of ethanol G: Glucan Incremental yield from xylan : 1.26 gallons of ethanol X: Xylan Total possible yield : 3.70 gal-ethanol (92% of theoretical yield, G+X) O:Other sugar 102.1% mass balance closure (solids + G + X + EtOH + Others) L: Lignin Other sugar is excluded for Ethanol production

Mass Balance of Soaking in Aqueous Ammonia (60 FPU/g-glucan)

Ammonia recycling
Ammonia

Biomass 100 lb (dry basis) G:36.1 lb X: 21.4 lb O: 7.8 lb L:17.2 lb

Treated stover

ARP Reactor
Liquid

Washing G: 36.1 lb X:19.1 lb

Hydrolysis

Soluble sugar X: 2.3 lb

Hydrolyzate

Glucose 37.3 lb Xylose 19.9 lb

Other sugar 7.8 Unhydrolyzed Sugar 7.2 lb Lignin 7.9 lb

Lignin 9.3 lb Residual solid 18.0 lb

Conversion Efficiency: 93.1% glucan + 81.7% xylan conversion at 60 FPU/g of glucan 101.3% mass balance closure

G: Glucan X: Xylan O: Other sugar L: Lignin

Mass Balance of Soaking in Aqueous Ammonia (15 FPU/g-glucan)

Ammonia recycling
Ammonia

Biomass 100 lb (dry basis) G:36.1 lb X: 21.4 lb O: 7.8 lb L:17.2 lb

Treated stover

Enzyme
Hydrolysis

ARP Reactor
Liquid

Washing G: 36.1 lb X:19.1 lb

Soluble sugar X: 2.3 lb

Hydrolyzate

Glucose 34.1 lb Xylose 17.6 lb

Other sugar 7.8 Unhydrolyzed Sugar 12.7 lb Lignin 7.9 lb

Lignin 9.3 lb Residual solid 18.0 lb

Conversion Efficiency: 85.0% glucan + 72.4% xylan conversion at 15 FPU/g of glucan 101.8% mass balance closure

G: Glucan X: Xylan O: Other sugar L: Lignin