Diseo de oligonucletidos con sitios de restriccin

A partir de la siguiente secuencia de ADN ACGACGGCGGCAAGCACCGCCAACCACCGAACATCAGACGCGTAGTCACAAACCACCATC ACATCAGTGATACGTTATTGAAAAAATAATAACTACTTATTAGCGCTTATGATTCACCCG CACTATAGTTCGATCATCGCTGCAGACGCAGCGATTGCGCCGGGGGCACCGGCGTCGGGC AACTGCATGGAGGTGGCATATGGCCGTCAAGACCTATAACGCGGGTGTGAAGGATTACCGG

F-Cbb3-X 5-ACGACGGCGTCTAGAACCGCC-3 R-Cbb3-X 5-CCGGTAATCCTCTAGACCCGCGTT-3 tm 62.1

tm

62.2

PRIMER FORWARD 5-ACGACGGCGTCTAGAACCGCC-3 SEQ GENOMA 5-ACGACGGCGGCAAGCACCGCC-3 comp !m!"t #61$25..61$%5& ACGACGGCGTCTAGAACCGCC ACGACGGCGGCAAGCACCGCCAACCACCGAACATCAGACGCGTAGTCACAAACCACCATC ACATCAGTGATACGTTATTGAAAAAATAATAACTACTTATTAGCGCTTATGATTCACCCG CACTATAGTTCGATCATCGCTGCAGACGCAGCGATTGCGCCGGGGGCACCGGCGTCGGGC AACTGCATGGAGGTGGCATATGGCCGTCAAGACCTATAACGCGGGTGTGAAGGATTACCGG 3-TTGCGCCCACACTTCCTAATGGCC-5 PRIMER '!(!')! SEQ GENOMA 61*+5..61*2, 5-CCGGTAATCCTCTAGACCCGCGTT-3 5-CCGGTAATCCTTCACACCCGCGTT-3

E p'omoto' p'!)!"t- -) )./0.!"t!) Coo'1!"-1-) 2!b'- m!"o) #61$%5.. 61*+5& T-m-3o 1! PCR 2%1 pb D./!)t.4" co" Xb-I 222 pb S!c0!"c.- o'./."!" p56 211 pb

#!)t- )!c0!"c.- 1!b! 0)-')! p-'- -"7 .).) 1! )!8&

TCTAGAACCGCCAACCACCGAACATCAGACGCGTAGTCACAAACCACCATCACATCAGTGATACGTTAT TGAAAAAATAATAACTACTTATTAGCGCTTATGATTCACCCGCACTATAGTTCGATCATCGCTGCAGAC GCAGCGATTGCGCCGGGGGCACCGGCGTCGGGCAACTGCATGGAGGTGGCATATGGCCGTCAAGACCTA TAACGCGGGTCTAGA