Cauliflower mosaic

virus
 Host Range

► Narrow
host range ,restricted
members of Brassicaceae

► Veinclearin in Arabidopsis,Brassica
spe,Rhaphanus sps &other plants
family in Brassicaceae
 Classification
► The Caulimoviridae are a family of viruses,
including the following genera:
► Genus Badnavirus; type species:
Commelina yellow mottle virus
► Genus Caulimovirus; type species:
Cauliflower mosaic virus
► Genus Tungrovirus; type species:
Rice tungro bacilliform virus
► Genus Soymovirus; type species:
Soybean chlorotic mottle virus
► Genus Cavemovirus; type species:
Cassava vein mosaic virus
► Genus Petuvirus; type species:
Petunia vein clearing virus
 Structure

► Camv is an icosahedral virus

► Diameter 50nm ,molecular weight 23*10(6)
dal
► genome is circular ds DNA,present between
► 2 & 3 layers
► Molecular weight 4.9*10(6)dal
► G+C content 43%
► Capsid multilayered
► Arrangement of capsomere not clear
Structure
► The (-)strand :single discontinuity or
gap
► The (+)strand:two gaps
► Gaps are primer binding sites for
replication of DNA
► Gaps are same respect to specific
restriction site
 Properties

► Thermal inactivation point 70 to 75

degree cell for 10 mint
► DNA resistant to chloroform
► Infectivity weakened by treatment
with ether
► Linear DNA not infective
► Serological studies:closely related to
dahila mosaic,straw berry banding and
carnation etched ring viruses
 Genome

► Coding region I -  codes for 38 KD protein which

helps in transfer of virus from one cell to another.
► Coding region II - codes for 19 KD protein which
attracts aphids.
► Coding region III - codes for viral 15 KD viral
capsomeres.
► Coding region IV - codes for 57 KD coat protein.
► Coding region V- codes for 79 KD reverse
transcriptase
► Coding region VI - codes for 58 KD proteins which
is used in building inclusion bodies.
► Coding region VII - codes for protein which
targets newly formed virus to inclusion body.
 Replication

► Enry into the host cell by aphids

► Uncoating and release of DNA within
cytoplasm
► Uncoated DNA transport to nucleus
► Removal of overhang and gaps by DNAse
and DNAligase
► DNA associated with histones
► Two RNA transcripts produced and
transported to cytoplasm
► 35sRNA Codes structural proteins
► 19sRNA involved replication
► dsDNA synthesised from RNAs by
reverse transcriptase
► Encapsidation occurs form progeny
virion
► Newly formed virus aggregate form
inclusion bodies
► Mature virions escape from infected
symptoms
 Symptoms

► Vein clearing or chlorotic vein banding

in Arabidopsis thaliana,Brassica spe.
► Mosaic symptoms occur in B.
campestris
► Stunting of entire plant
► Virus containing Inclusion bodies in the
cytoplasm
► Nucleus become lobed
► vesicles protrude out from cell wall
 Control

► Using insecticides
► Elimination of disease plants
► Cultivation of virus –resistant varieties
► Selection of disease free planting
materials
► Selection of disease free soil for
cultivation
 APPLICATIONS
► The cauliflower mosaic virus (CaMV) 35S promoter
has been most commonly used in plant
transformation studies, but its activity in
mushrooms has not been reported.
► p301-b is a binary vector containing a bialaphos
resistance gene driven by the promoter of Lentinus
edodes glyceraldehyde-3-phosphate
dehydrogenase (GPD) gene.
► CaMV 35S-GUS was inserted into p301-b, and the
resulting construct p301-bG was transformed to
protoplasts of Ganoderma lucidum and
basidiospores of Pleurotus citrinopileatus.
► GUS activity was observed in the transformants,
indicating that CaMV 35S promoter can direct
expression of exogenous gene in the mushrooms.
This is the first report on the application of CaMV
35S promoter in genetic modification of