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  • Protein Estimation by Lawry's Method: Unknown Samples

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    vijaygovindaraj
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    This document summarizes a protein estimation method called Lawry's method. It includes a table showing sample readings from a protein standard curve experiment using bovine serum albumin at concentrations from 10-50 ug. The table includes absorbance readings at 680nm for the standard curve in duplicate, as well as absorbance readings for 4 unknown protein samples. It also shows the linear regression equation derived from the standard curve as y=0.0098x + 0.0253, which can be used to calculate the protein concentrations of the unknown samples.

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    Excel format which is useful for students

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    Lowry'sMethod

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    This document summarizes a protein estimation method called Lawry's method. It includes a table showing sample readings from a protein standard curve experiment using bovine serum albumin at concentrations from 10-50 ug. The table includes absorbance readings at 680nm for the standard curve in duplicate, as well as absorbance readings for 4 unknown protein samples. It also shows the linear regression equation derived from the standard curve as y=0.0098x + 0.0253, which can be used to calculate the protein concentrations of the unknown samples.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Scarica in formato XLS, PDF, TXT o leggi online su Scribd
    Segnala contenuti inappropriati
    23 visualizzazioni3 pagine

    Protein Estimation by Lawry's Method: Unknown Samples

    Caricato da

    vijaygovindaraj
    This document summarizes a protein estimation method called Lawry's method. It includes a table showing sample readings from a protein standard curve experiment using bovine serum albumin at concentrations from 10-50 ug. The table includes absorbance readings at 680nm for the standard curve in duplicate, as well as absorbance readings for 4 unknown protein samples. It also shows the linear regression equation derived from the standard curve as y=0.0098x + 0.0253, which can be used to calculate the protein concentrations of the unknown samples.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Scarica in formato XLS, PDF, TXT o leggi online su Scribd
    Segnala contenuti inappropriati
    di 3

    Protein Estimation by Lawry's Method

    1
    2
    3
    4
    5
    Unknown
    Samples

    1
    2
    3
    4
    5
    6
    7
    8
    9
    10

    Protein
    Standard
    (ug)

    DDH20

    AlCuSO4

    (ul)

    (ul)

    FC
    Reagent
    (ul)

    10
    20
    30
    40
    50

    190
    180
    170
    160
    150

    1000
    1000
    1000
    1000
    1000

    100
    100
    100
    100
    100

    Preoptic C
    Preoptic T
    Uterus T
    Uterus T

    #VALUE!
    #VALUE!
    #VALUE!
    #VALUE!

    1000
    1000
    1000
    1000

    100
    100
    100
    100

    Formula

    Y=mx+b
    Y
    UK OD
    m
    0.009
    x
    b
    0.025

    Unknown Conc
    Slope Value
    Protein Conc
    y intercept

    Control-1
    control-2
    Tumor-1
    Tumor-2

    150ug
    83.20493066
    28.58655373
    54.60060667
    6000

    Buffer
    -53.2049307
    1.41344627
    -24.6006067
    -5970

    Dye

    300ug
    166.4098613
    57.17310746
    109.2012133
    12000

    Buffer
    Dye
    -136.409861
    -27.1731075
    -79.2012133
    -11970

    150ug
    X6 Acetone
    499.2296
    171.5193
    327.6036
    36000
    150ug
    X6 Acetone
    998.4592
    343.0386
    655.2073
    72000

    OD
    OD
    680nm
    680nm
    Duplicate Duplicate
    1
    2
    0.158
    2
    0.299
    2
    0.415
    2
    0.54
    2
    0.67
    2

    Average
    OD
    0.129
    0.22
    0.313
    0.405
    0.525
    0.016
    0.047
    0.0245

    400ug
    4.807407
    13.99259
    7.325926
    0.066667

    acetone
    6 vol
    28.84444
    83.95556
    43.95556
    0.4

    x=y-b/m
    Unknown
    Protein
    Concentration

    1.802777778
    5.247222222
    2.747222222
    0.025
    0.025
    0.025
    0.025
    0.025
    0.025
    0.025

    Data from Lowry Assay for Protein


    y = 0.0098x + 0.0253
    R = 0.9967
    0.6

    0.525
    0.5

    0.405

    OD at 680nm

    0.4

    0.313

    0.3

    0.22
    0.2

    0.129
    0.1

    0
    0

    10

    20

    30

    Protein Concentration (ug/ul)

    40

    50

    60

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