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Descriptive Chart

Culture No.: 61 Organism: Proteus vulgaris

Morphological Characteristics Cell Shape: Bacillus (rod) Arrangement: swarm Size: 0.4 to 0.6 micrometers by 1.2 to 2.5 micrometers Spores: no Grams Stain: negative Motility: yes Capsules: no Cultural Characteristics Nutrient Agar: Colonies clumped together Blood Agar: Positive for hemolysis Agar Slant: positive for growth Nutrient Broth: positive for growth Gelatin Slab: positive for motility and liquefaction Oxygen Requirements: facultative anaerobic Optimum Temp.: 23 C Physiological Characteristics

Tests

Results

IMViC Hydrolysis Fermentation

Glucose Lactose Sucrose Mannitol Gelatin Liquefaction Starch Casein Fat Indole Methyl Red V-P (acetylmethylcarbinol) Citrate Utilization Nitrate Reduction H2S Production Urease Catlase Oxidase DNase Phenylalanase

Yes; yellow No; orange/pink Yes; yellow/orange No; orange/pink Positive Negative Positive Positive Positive (red floating layer) Yes Negative Negative Positive after Zn added Positive (black) Positive (light pink) Yes Negative ?postive (slight green)

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