HIGH PERFORMANCE THIN LAYER CHROMATOGARPHY(HPTLC)

BY M.PAUL RICHARDS MPAQA

WHAT IS PLANAR /THIN LAYER CHROMATOGRAPHY

THE PRINCIPLE
SAMPLE APPLICATION CHROMATOGRAM DEVELOPMENT CHROMATOGRAM EVALUATION

ADVANTAGES OFFERED BY HPTLC

SAMPLE APPLICATION / SAMPLE DOSAGE

NATURE OF THE ANALYTICAL TASK. TYPE OF SEPERATION LAYER . SAMPLE VOLUMES.

NANOMAT

Capillary Dispenser System

LINOMAT 1V

AUTOMATIC TLC SAMPLER

CHROMATOGRAM DEVELOPMENT
GENERAL

Ancillaries for Chromatogram Development in a tank

Flat Bottom Chamber

Twin Trough Chamber

LOW SOLVENT CONSUMPTION

pre-equilibration with solvent vapor

Start of development

LIGHT WEIGHT TWIN TROUGH CHAMBER

Horizontal Developing Chamber

Automatic Developing Chamber ADC

AMD System

DENSITOMETRIC CHROMATOGRAM EVALUATION

General, Comparsion of Video Technology with Classical Densitometry.

TLC Scanner 3 with CATS Soft Ware.

 CATS STANDARD PROGRAM. CATS PROGRAM OPTIONS

Photo & Video Documentation / Video Densitometry.

Reprostar.

Video store.

Video Scan.

OPTIONAL (AUXILLARY) PROCEDURES:

UV INSPECTION - UV Lamps. POST CHROMATOGRAPHIC DERIVATIZATION. OPTIMIZATION OF DEVELOPING CONDITION. ALTERNATIVE TECHNIQUES FOR MNUAL SAMPLE APPLICATION. IN HOUSE PREPARATION OF TLC PLATES.

UV INSPECTION -UV Lamps.

POST CHROMATOGRAPHIC DERIVATIZATION General. Chromatogram Immersion Device. TLC Sprayer. Reagent Spray. TLC Spray Cabinet. TLC Plate Heater

Chromatogram Immersion Device.

TLC Sprayer.

Reagent Spray.

TLC Spray Cabinet.

TLC Plate Heater

OPTIMIZATION OF DEVELOPING CONDITION.

HPTLC VARIO SYSTEM.

ALTERNATIVE TECHNIQUES FOR MNUAL

SAMPLE APPLICATION
Disposable Glass Capillaries Graduated Disposable Micro Pipettes Multipurpose Spotting Guide.

Disposable Glass Capillaries

Graduated Disposable Micro Pipettes

IN HOUSE PREPARATION OF TLC PLATES.

GENERAL. Automatic TLC Plate Coater, TLC PLATE Coater, Hand operated, Glass Plates , Drying rack, TLC Plate Box .

Automatic TLC Plate Coater

TLC PLATE Coater, hand operated

TLC Plate Box.

Drying rack

ADSORBENTS FOR IN HOUSE

PREPARATION OF TLC LAYERS.

General CAMAG Adsorbents. MERCK Adsorbents.

CAMAG Adsorbents.

032.3301 032.3305 032.3351 032.3355 032.3401 032.3405 032.3451 032.3455 032.3502 032.3512

Aluminium Oxide CAMAG DS-0 Aluminium Oxide CAMAG DS-0 Aluminium Oxide CAMAG DS-5 Aluminium Oxide CAMAG DS-5 Aluminium Oxide CAMAG DSF-0 Aluminium Oxide CAMAG DSF-0 Aluminium Oxide CAMAG DSF-5 Aluminium Oxide CAMAG DSF-5 Cellulose, fibre type, CAMAG D-0 Cellulose, fibre type, CAMAG DF-0

1 kg 5 kg 1 kg 5 kg 1 kg 5 kg 1 kg 5 kg 500 g 500 g

032.3552
032.3562 032.1001 032.1005 032.1051 032.1055 032.1101 032.1105 032.1151 032.1155

Cellulose, microcrystalline, CAMAG DS-0

Cellulose, microcrystalline, CAMAG DFS-0 Silica Gel, CAMAG DS-0 Silica Gel, CAMAG DS-0 Silica Gel, CAMAG DS-5 Silica Gel, CAMAG DS-5 Silica Gel, CAMAG DSF-0 Silica Gel, CAMAG DSF-0 Silica Gel, CAMAG DSF-5 Silica Gel, CAMAG DSF-5

500 g
500 g 1 kg 5 kg 1 kg 5 kg 1 kg 5 kg 1 kg

MERCK Adsorbents
033.1090 033.1092 033.1091 033.1085 033.1094 033.7731 033.7733 033.7730 033.7729 033.7736 033.7738 033.7745 033.7739 033.7741 033.7744 033.8129 Aluminium Oxide MERCK G (type 60/E) Aluminium Oxide MERCK GF (type 60/E) Aluminium Oxide MERCK G (type 60/E) Aluminium Oxide MERCK H basic (type 60/E) Aluminium Oxide MERCK HF basic (type 60/E) Silica Gel MERCK G (type 60) Silica Gel MERCK G (type 60) Silica Gel MERCK GF (type 60) Silica Gel MERCK GF (type 60) Silica Gel MERCK H (type 60) Silica Gel MERCK H (type 60) Silica Gel MERCK HF (type 60) Silica Gel MERCK HF (type 60) Silica Gel MERCK HF 254 + 366 (type 60) Silica Gel MERCK 60 HR "high purity" Kieselguhr MERCK 60 G 500 g 500 g 2,5 kg 500 g 500 g 1 kg 5 kg 1 kg 5 kg 1 kg 2,5 kg 500 g 1 kg 1 kg 500 g 500 g

PRECOATED LAYERS

MERCK Precoated layers for HPTLC.

034.5035 034.5036 HPTLC plates cellulose F HPTLC plates cellulose F 100 100 10x10 20x10 25 50

034.5787
034.5786 034.5631 034.5633 034.5641 034.3748 034.3749 034.5628 034.5629 034.5564 034.5642

HPTLC plates cellulose (without F)

HPTLC plates cellulose (without F) HPTLC plates silica gel 60 (without F) HPTLC plates silica gel 60 (without F) HPTLC plates silica gel 60 (without F) HPTLC plates silica gel 60 (without F), concentration zone HPTLC plates silica gel 60 (without F), concentration zone HPTLC plates silica gel 60 F 254 HPTLC plates silica gel 60 F 254 HPTLC plates silica gel 60 F 254 GLP HPTLC plates silica gel 60 F 254

100
100 200 200 200 200 200 200 200 200 200

10x10
20x10 10x10 10x10 20x10 10x10 20x10 10x10 10x10 10x10 20x10

25
25 25 100 50 25 50 25 100 25 50

034.1764
034.3727 034.3728 034.5613 034.1552 034.5445 034.5547

HPTLC plates silica gel 60 F 254, extra thin layer

HPTLC plates silica gel 60 F 254, concentration zone HPTLC plates silica gel 60 F 254, concentration zone HPTLC plates silica gel 60 F 254 GLP HPTLC plates silica gel WR 60 F 254s HPTLC plates LiChrospher Si 60 F 254s HPTLC aluminium sheets silica gel 60 (without F)

100
200 200 200 200 180 200

20x10
10x10 20x10 20x10 20x10 20x10 20x20

25
25 50 25 25 25 25

MERCK Precoated Layers for (Conventional)

034.5713 034.5718 034.5716 034.5552 034.5724 034.5721 034.1844 034.1845 034.5729 034.5715 034.5566 034.1846 034.1798 034.5554 034.5553 034.5582 034.5583 034.5735 TLC plates aluminium oxide 60 F 254 TLC plates cellulose F TLC plates cellulose (without F) TLC aluminium sheets cellulose (without F) TLC plates silica gel (without F) TLC plates silica gel (without F) TLC plates silica gel (without F), concentration zone TLC plates silica gel (without F), concentration zone TLC plates silica gel 60 F 254 TLC plates silica gel 60 F 254 TLC plates silica gel 60 F 254 GLP TLC plates silica gel 60 F 254, concentration zone TLC plates silica gel 60 F 254, concentration zone TLC aluminium sheets silica gel 60 F 254 TLC aluminium sheets silica gel 60 (without F) TLC aluminium sheets silica gel 60 (without F), concentration zone TLC aluminium sheets silica gel 60, concentration zone TLC plastic sheets silica gel 60 F 254

TLC.

250 100 100 100 250 250 250 250 250 250 250 250 250 200 200 200 200 200

20x20 20x20 20x20 20x20 5x20 20x20 10x20 20x20 10x20 20x20 20x20 10x20 20x20 20x20 20x20 20x20 20x20 20x20

25 25 25 25 100 25 25 25 50 25 25 50 25 25 25 25 25 25

034.5748
034.5423

TLC plastic sheets silica gel 60 (without F)

TLC plates RP-18 F 254s

200
250

20x20
10x20

25
50

Test Dye Mixtures

032.8001
032.8002

Test Dye Mixture I 100 mL (suitable for silica gel) Dimethyl yellow - Oracet blue 2R - Oracet red G; solvent: toluene
Test Dye Mixture II 100 mL (suitable for silica gel and aluminium oxide) Sudan black - Artisil blue - Sudan yellow - fatty orange - fat red 7B - (Sudan black separates into two fractions); solvent: toluene Test Dye Mixture III 10 mL (suitable for HPTLC silica gel) Ciba F II - Indophenol - Ariabel red - Sudan blue II - Sudan IV - dimethylaminoazobenzene; solvent: toluene

032.8003

032.8004

Test Dye Mixture IV 50 mL (MERCK 9352, hydrophylic) Chrysoin S - fast yellow - Amaranth - brilliant black BN; solvent: methanol - water 1:1

APPLICATIONS
BIOCHEMICAL RESEARCH. CLINICAL. COSMETICS. ENVIRONMENTAL ANALYSIS. FOOD ANALYSIS. PHARMACEUTICALS. NATURAL PRODUCTS, PLANT INGREDIENTS. TOXICOLOGY, FORENSIC ANALYSIS. DOPING ANALYSIS.