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Virology Biology W3310/4310 Spring 2013 Prof. V.

Racaniello Study Questions for lecture 8 Transcription and RNA processing 1. What is transcription? What are the generic steps in this process? 2. What happens to RNA transcripts? 3. Where in the cell does transcription occur? Is this true for all viruses? 4. What is the purpose of splicing? Provide an example. 5. It is known that splicing marks cellular mRNAs for nuclear export. How are unspliced viral mRNAs exported from the nucleus? 6. What is a viral transcriptional activator? How does it function? 7. Describe positive vs negative autoregulatory loops. 8. What is a transcriptional cascade and why is it necessary?

Study questions W3310/4310

Prof. V. Racaniello

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