VIN NN ACID FOLIC Tabellae Acidi folici L vin nn cha acid folic.

Ch phm phi p ng cc yu cu trong chuyn lun Thuc vin nn (Ph lc 1.20) v cc yu cu sau y: Hm lng acid folic, C19H19 N7O6, t 90,0 n 110,0 % so vi hm lng ghi trn nhn. Tnh cht Vin nn mu vng hoc vng cam. nh tnh Phng php sc k lp mng (Ph lc 5.4). Bn mng: Silicagel G. Dung mi khai trin: Amoniac 13,5 M - propanol - ethanol 96% (20 : 20 : 60). Dung dch th: Ha tan mt lng bt vin tng ng vi 0,5 mg acid folic trong 1 ml hn hp gm 1 th tch amoniac 13,5 M (TT) v 9 th tch methanol (TT) . Ly tm v ly dung dch trong chm sc k. Dung dch i chiu: Dung dch acid folic chun 0,05% trong hn hp gm 2 th tch amoniac 13,5 M (TT) v 9 th tch methanol (TT) . Cch tin hnh: Chm ring bit ln bn mng 2 l mi dung dch trn. Trin khai sc k n khi dung mi i c khong 15 cm. Ly bn sc k ra, kh ngoi khng kh v quan st di nh sng t ngoi bc sng 365 nm. Vt chnh trn sc k ca dung dch th phi tng ng v v tr, mu sc hunh quang v kch thc vi vt chnh trn sc k ca dung dch i chiu. ng u hm lng Vin nn cha t hn 2 mg acid folic phi p ng yu cu ng u hm lng (Ph lc 11.2) Phng php sc k lng (Ph lc 5.3). Tin hnh th nghim trong iu kin trnh nh sng. Pha ng: Hn hp dung dch kali dihydrophosphat 0,05 M (TT) v acetonitril (TT) (93 : 7) c iu chnh n pH 6,0 bng dung dch natri hydroxyd 5 M (TT). Dung dch chun: Thm 1 ml dung dch acid hydrocloric 0,5 M (TT) vo 5 ml dung dch acid folic chun 0,0020 % trong dung dch natri hydroxyd 0,1 M (TT) v pha long thnh 10 ml bng pha ng . Dung dch th : Lc 1 vin vi 5 ml dung dch natri hydroxyd 0,1 M (TT) v thm va pha ng c dung dch c nng acid folic 0,001 %. Ly tm v s dng dung dch trong. iu kin sc k Ct thp khng r (20 cm x 4,6 mm) cha pha tnh C (ct Spherisorb C18, 10 m l thch hp). Detector quang ph t ngoi t bc sng 283 nm. Tc dng: 2 ml/pht. Th tch tim: 10 l. Tnh hm lng C19H19 N7O6 trong tng vin da theo hm lng C19H19 N7O6 ca dung dch chun. nh lng Phng php sc k lng (Ph lc 5.3). Tin hnh th nghim trong iu kin trnh nh sng. i vi vin nn cha 2 mg acid folic hoc nhiu hn . Dung dch chun: Pha long 5 ml dung dch acid folic chun 0,020% trong dung dch natri hydroxyd 0,1 M (TT) thnh 100 ml bng pha ng. Dung dch th: Cn 20 vin, tnh khi lng trung bnh ca vin, nghin thnh bt mn. Lc mt lng bt vin tng ng khong 20 mg acid folic vi 50 ml dung dch natri hydroxyd 0,1 M (TT), pha long

bng dung dch natri hydroxyd 0,1 M (TT) thnh 100 ml, ly tm v pha long 5ml dch trong thnh 100 ml bng pha ng. iu kin sc k: Nh m t trong mc ng u hm lng. Tnh hm lng C19H19 N7O6 trong mi vin da theo hm lng C19H19 N7O6 ca dung dch chun. i vi vin nn cha t hn 2 mg acid folic. Ly gi tr trung bnh ca 10 vin trong th nghim ng u hm lng. Sn phm phn hy Phng php sc k lng (Ph lc 5.3). Tin hnh th nghim trong iu kin trnh nh sng. Pha ng: Dung dch kali dihydrophosphat 0,05 M (TT) c iu chnh n pH 5,5 bng dung dch natri hydroxyd 5 M (TT). Lc qua mng lc 0,45 m v ui kh. Dung dch i chiu: Dung dch cha 0,5 g/ml acid 4- aminobenzoic v 2,0 g/ml acid N-(4aminobenzoyl)-L-glutamic trong pha ng. Dung dch th: Lc mt lng bt vin cha 5,0 mg acid folic vi 50 ml pha ng, ly tm v s dng dung dch trong. iu kin sc k: Ct thp khng g (20 cm x 4,6 mm) c nhi pha tnh C (ct Spherisorb C18, 10 m l thch hp). Detector quang ph t ngoi t bc sng 269 nm. Tc dng: 2 ml/pht. Th tch tim: 10 l. Trong sc k ca dung dch i chiu s c 2 pic tng ng vi acid N-(4-aminobenzoyl)-L-glutamic v acid 4-aminobenzoic. Php th ch c gi tr khi h s phn gii gia 2 pic khng c nh hn 3. Din tch ca cc pic tng ng vi acid 4-aminobenzoic v acid N-(4-aminobenzoyl)-L-glutamic trong sc k thu c ca dung dch th khng c ln hn din tch ca cc pic tng ng trong sc k ca dung dch i chiu. Bo qun ng trong bao b kn, ni kh mt, trnh nh sng. Loi thuc Vitamin Hm lng thng dng 0,4 mg, 0,8 mg, 1 mg, 5 mg