VIN NN ACID ASCORBIC Tabellae Acidi ascorbici Vin nm Vitamin C L vin nn cha acid ascorbic.

Ch phm phi p ng cc yu cu trong chuyn lun "Thuc vin nn" (Ph lc 1.20) v cc yu cu sau y : Hm lng acid ascorbic, C6H8O6, t 95,0 - 110,0% so vi hm lng ghi trn nhn. Tnh cht Vin mu trng hay trng ng. nh tnh A.Cn mt lng bt vin tng ng vi khong 0,10 g acid ascorbic, thm 10 ml nc. lc k, Lc. Dch lc c phn ng acid vi giy qu (TT). Ly 5 ml dch lc thm 0,5 ml dung dch bc nitrat 2 % (TT), xut hin ta xm en. B.Phng php sc k lp mng (Ph lc 5.4). Bn mng: Silica gel GF 254. Dung mi khai trin: Ethanol 96 % - nc (120 : 20). Dung dch th: Cn mt lng bt vin tng ng vi khong 0,05 g acid ascorbic, thm 10 ml nc, lc k v lc. Dung dch i chiu: Dung dch acid ascorbic chun 0,5% trong nc. Cch tin hnh: Chm ring bit ln bn mng 2 l mi dung dch trn. Trin khai sc k n khi dung mi i c khong 15 cm. Ly bn mng kh ngoi khng kh nhit phng. Quan st di nh sng t ngoi bc sng 254 nm. Sc k thu c ca dung dch th phi c vt tng ng v v tr v mu sc vi vt chnh trong sc k thu c ca dung dch i chiu. nh lng Cn 20 vin, tnh khi lng trung bnh v nghin thnh bt mn. Cn mt lng bt vin tng ng vi khong 0,1 g acid ascorbic, thm 30 ml hn hp nc un si ngui v dung dch acid acetic 1M (TT) (10 : 1), lc k. Thm 1 ml dung dch h tinh bt (TT), nh lng bng dung dch iod 0,1 N (C) cho ti khi xut hin mu xanh lam bn vng. 1 ml dung dch iod 0,1 N (C) tng ng vi 8,806 mg C6H8O6. Bo qun Trnh m v nh sng, trnh tip xc vi kim loi. Lai thuc Vitamin. Hm lng thng dng 500 mg, 1000 mg.