Catalog Phenomenex 2012

30
Celebrating
years of
Ingenuity, Imagination,
and Heart
Chromatography
Product Guide 12/13
Ingenuity, Im
a
gin
30
atio
ng
brati 30 Yea
ele
r
f
so
n, and H
ea
r
Lets face it.

Chromatography is not sexy, or glamorous.
Or is it?
From our humble beginnings, with no money and no technology, we set out
to create a different type of scientific company. We asked ourselves, Why
do we have to be another boring chromatography company? Could we reinvent approaches in research, customer care, and employee welfare?
You see, when you start off as the underdog, you can either choose to copy
or decide to approach everything differently. We, of course, chose to rethink
everything. Our slogan, breaking with tradition was born from that thought
process and it still shapes our decisions today. We are proud we took the
more difficult path as we are confident the results are more ingenious, more
imaginative, and full of more heart than would have ever been possible if we
had just followed the (former) leaders of the pack.
Because it has been 30 years, we figured this milestone would be a good
opportunity to share a little more about who we are, what we stand for, and
to see if you think were on the right track.
After all, without YOU, who are we?
Lets get started! In the past three decades
We advanced.
And by advanced, we mean technologically. Over the years, we have introduced cutting-edge technologies and services that have changed the face
of separation science.
Just a few highlights...
1986
1988
1990
1982
We open our doors as an HPLC
column distributor
1986
We became an HPLC column
manufacturer
1996
Introduce Luna which quickly
becomes the worlds
#1 HPLC Column
1997
Acquired GC column manufacturing
1998
1999
2002
2006
Clarity BioSolutions
for Synthetic DNA/RNA
2000
Diversified into SPE manufacturing
2004
Free app for your mobile:
http://www.gettag.mobi
2007
2008
2009
Introduced the first and only
sub-2m core-shell technology
2012 Phenomenex, Inc. All rights reserved.
2010
2011
Applied market-leading core-shell
technology for BioSeparations and
introduced PhenoLogix method
development services
2012
The Future
Many more exciting technologies
in our pipeline for years to come!
We expanded.
Back in the fluorescent days of 1982, we started selling
columns out of a garage. Over the course of time, we slowly
and strategically planned how and where we would expand.
By 2011, we had opened 3 manufacturing sites, spread
into 10 buildings at our California headquarters, opened
15 international subsidiary offices (including our newest in
Hyderabad, India), and partnered with over 65 distributors
to ensure worldwide availability of our products. And what
about the future you might ask? We plan to accelerate our
growth even more!
Corporate Office
Corporate Offices
Buildings 1, 2, 3, 5, 6, 7, and 10 (shown). Present day.
Mr. Mahjoors Garage. Circa 1982
Italy
Germany
France
India
Australia
UK
Denmark
The Netherlands
New Zealand
InventX (GC Manufacturing)
Wibby Environmental
For the sake of humanity, visit page 60.

Phenomenex
Dear M
411 Ma
drid Av
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90501-1 CA
430
USA
Tel: 31
0-212-0
Fax: 31
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Email:
0-328-7 55
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United
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Phenomenex
SERVICES
Filtration
Table of
Contents
58
9 18
Pipettes
19 20
Vials
21 34
Sample Preparation / spe
35 59
GC Columns and Accessories
61 143
HPLC
144 264
UHPLC
265 276
BIOSEPARATIONS
277 310
chiral LC
311 326
supercritical fluid
chromatography (SFC)
327 333
Prep Columns
and Bulk Media
335 352
ACCESSORIES
and Consumables
353 378
APPENDICES
379 408
Amino Acids...........................................................................278 282

Proteins / Peptides..............................................................283 300
Synthetic DNA / RNA.............................................................301 310
Our
Mission
The very existence of Phenomenex depends directly on the complete satisfaction of our customers. Therefore, it is our duty to acknowledge the contributions made by our customers and our staff
toward the progress of the company. Further, it is our responsibility and foremost mission to promote the growth, prosperity, and wellbeing of those we serve our customers, our employees, and humanity.
Fasha Mahjoor
CEO and Founder
Founding
Beliefs and Principles

of Phenomenexs CEO
The oneness of humankind

The abolition of all forms of prejudice such as those based on race,
gender, religion, nationality, or class
Upholding equal opportunity, rights and privileges for men and
women
The essential harmony of scientific and spiritual truths, which could
constitute the ultimate basis for a peaceful, ordered, and progressive society
Universal compulsory education for all children
Economic justice
Protection of human rights
Protection of endangered species
Protection of the environment
Phenomenex
services
Services
Services
PhenoLogix ............................................................................................... 6
5-8
Services
phenologix | services
PhenoLogix:
A NEW Analytical Support Laboratory
Personalized Solutions
Experienced Staff
Fast Project Turn Around Time
Confidential
Immediate Availability
Award Winning Customer Service
PhenoLogix is a full-service analytical support laboratory within Phenomenex that specializes in method development and optimization for HPLC, UHPLC, GC, GC/MS, LC/MS/MS,
and SPE. Our team of highly experienced scientists provides customized solutions to help
streamline your development efforts.
PhenoLogix is committed to making the experience of working together easy, effortless, and
rewarding.
Get started today, and let us exceed your expectations.
We are as passionate about science as

you are, and treat your projects as we
would our own.
Jeff Layne, Ph.D.
Technical Manager, PhenoLogix
Phenomenex
Services
How Can We Be of Service?
New Method Development and Optimization
Method Improvement (Without the Need for Re-Validation)
Validation Services, Including FDA Compliant cGMP Validations
Chiral Screening and Optimization
Analytical to Preparative HPLC Scale Up
services | phenologix
HPLC | UHPLC | GC | GC/MS | LC/MS/MS | SPE |

GFC | SFC | CHIRAL | GPC | IEX | IC
Small Scale Purification (< 2 g)

Hands-On Training
Consulting
On-Site Method Transfer
PhenoLogix offers different levels of support based on your companys needs, ranging from simple
column screening services to full FDA compliant cGMP validations. We are also more than happy
to send one of our scientists to your lab to help solve a problem hands-on or to provide training
seminars and professional consulting services.
A majority of our service offerings are free of charge. For those requiring an additional cost, pricing
will be disclosed and approved prior to beginning a project.
Phenomenex
Services
phenologix | services
Get Started Today We Make it Easy

We guarantee a great experience when working with our team. All of our project
managers are enthusiastic and dedicated to you, our valued customer.
Contact Us
1
Speak with one of our friendly PhenoLogix team members or

log onto our website at
www.phenomenex.com/phenologix
USA/CAN/MX: PhenoLogix@phenomenex.com
EUROPE: PhenoLogixEUR@phenomenex.com
ASIA: PhenoLogixASIA@phenomenex.com
Confidentiality
If necessary, put a confidentiality agreement and/or other
documentation into place.
Tell Us About The Project

2
Provide information on your project such as compound

structure, specific requirements, and separation goals.
Most Services are Free

If a cost is involved, you will receive a customized plan, pricing,
and timeline for each individual project for your approval.
Tell Us When to Start

3
Approve our proposal and well get to work on your project!
Open Lines of Communication

While working on your project, we will hold regular meetings to
update you on our progress until you are satisfied with the final
method.
Jix,
Cool Mascot
Project is Complete
4
A detailed report is sent to you, including full method,

materials, chromatograms, and discussion.
Method Transfer
We make sure the method works in your laboratory either
by supporting your efforts via teleconference or sending a
PhenoLogix representative to your lab. We dont finish until
you are happy!
Relax!
Wonder what Im listening to?
www.phenomenex.com/phenologix
Phenomenex
Filtration Products
Sample Handling
and Mobile Phase
Filtration
Filtration Products
Centrifugal Filters .................................................................................... 12
Column Couplers .................................................................................... 18
Filter Membranes .................................................................................... 15

Inlet Filters

Metal-free / Biocompatible................................................................ 16
Stainless Steel................................................................................... 16
In-Line Filters

Biocompatible PEEK.................................................................... 17-18
Stainless Steel.............................................................................. 17-18
Mobile Phase Filtration Accessories........................................................ 14
Mobile Phase Filtration System............................................................... 13
Solvent Pickup Adapter........................................................................... 14
Syringe Filters
Non-sterile.................................................................................... 10-11
Sterile................................................................................................. 12
Syringes, Disposable............................................................................... 12
9 - 18
Filtration
Syringe Filters
best seller
Phenex
Rapid filtration of HPLC and GC samples prior to analysis

Particulate, PVC, and extractable-free filters
Less system downtime
Phenex Offers:
More consistent, reproducible results

Increased column lifetime
Syringe Filter Selection Guide
Broad chemical compatibility

Minimized extractables
Excellent flow rate
High total throughput
Certified quality
100 % integrity tested

Low hold-up volume
Low protein adsorption
Bi-directional use
Syringe Filters
FILTRATION - Sample handling
For sample and solvent filtration prior to

chromatography
1. Choose filter diameter based on sample volume

4 mm Diameter
2 mL Sample Volume
15 mm Diameter
2 - 10 mL Sample Volume
25 - 28 mm Diameter
10 - 100 mL Sample Volume
2. Choose a pore size based on the nature of your sample and chromatographic method
Sample Description
Recommended Filter Pore Size
General aqueous or mixed organic samples prior to HPLC analysis with columns packed with > 3 m particles.
General clarification of GC, SFC, CE, and GPC samples.
0.45 m
Viscous samples or samples containing high levels of particulate matter.

General aqueous or mixed organic samples prior to HPLC analysis with columns packed with < 3 m particles.
Removal of fine particulate matter prior to GC, SFC, CE, and GPC samples.
0.20 m
Gas samples prior to GC. Liquid samples prior to UHPLC or LC/MS. Other particulate-sensitive methods.
Viscous samples such as serum, plasma or other biological matrices. Solutions with high particulate load such
as some environmental, biofuels or food and beverage applications.
Glass Fiber Filter

with 0.45 m filter
3. Choose a filter membrane according to the characteristics of your sample and filtering objective
Membrane Type
Recommended Uses
RC
Hydrophilic Regenerated Cellulose filter membranes are compatible with a very broad range of aqueous and mixed-organic solutions,
making them one of the most universal filter materials used prior to chromatography. Phenex-RC filters also exhibit fast-flow and
ultra-low protein and non-specific binding characteristics. Due to the beneficial material characteristics, Phenex-RC membranes are
broadly recommended as an excellent general purpose/high-performance sample filter for most applications.
(Regenerated Cellulose)
PTFE, Teflon
(Polytetrafluoroethylene)
PES
(Polyethersulfone)
NY
(Nylon)
CA
(Cellulose Acetate)
GF
(Glass Fiber)
10
PTFE is an inherently hydrophobic membrane excellent for filtration of organic-based, highly acidic or basic samples and solvents.
Widely used in chromatography, it is especially well suited for the clarification of non-aqueous samples. Although this membrane is
hydrophobic, it can be made hydrophilic by wetting the membrane with alcohol and then flushing with deionized water.
Polyethersulfone membranes exhibit very fast-flow and ultra-low protein binding characteristics and are ideally suited for use in many
life science clarification applications. Phenex-PES membranes typically offer better chemical resistance than cellulose acetate and
are broadly recommended for filtering critical biological samples, tissue culture media, additives and buffers.
Nylon has inherent hydrophilic characteristics and works well for filtration of many aqueous and mixed-organic samples. In combination with a glass pre-filter (Phenex-GF/NY), this membrane is excellent for the filtration of particle-laden samples, such as foods
and beverages, environmental, biofuels, and dissolution samples. For applications that require low protein or non-specific binding
characteristics, Phenomenex recommends Phenex-RC (Regenerated Cellulose) filters.
Cellulose Acetate membranes exhibit ultra-low protein binding and are broadly used in the filtration of biological samples. In
combination with a glass pre-filter (Phenex-GF/CA), this membrane is excellent for filtration of tissue culture media, general biological
sample filtration and clarification.
Phenex-GF (Glass Fiber) filters are made of inert borosilicate glass and have a nominal 1.2 m pore size. They are commonly used
with highly viscous samples or samples that contain high concentrations of particulate matter (e.g., food analysis, biological samples,
soil samples, fermentation broth samples, removal of yeasts, molds, etc.). Glass Fiber filters can be used alone or in series with other
Phenex filter membranes such as the 0.45 m pore Phenex-RC filter to reduce clogging of the membrane and optimize flow.
Phenomenex
Filtration
If Phenex Syringe Filters do not perform
as well or better than your current syringe
filter product of similar membrane,
diameter and pore size, return the
product with comparative data within 45
days for a full refund.
Syringe Filters (contd)

Syringe Filter Applications and Recommended Membranes
Ordering Information
Recommended
Filter
RC
PTFE
PES
GF/NY
GF/NY
GF/NY
RC
GF/NY
RC
RC
RC
GF/CA
First
Alternative
Second
Alternative
PTFE
RC
RC
GF + RC
RC
RC
PES
RC
PES
PES
PES
PES
PES
NY
GF/CA
PTFE
PTFE
PTFE
NY
PTFE
PTFE
NY
NY
RC
4 mm Diameter
for 2 mL sample volumes
Phenex Syringe Filters
Tip: Try a Sample Pack!

Request yours today by phone or visit
www.phenomenex.com/sample
For high load and particulate-laden samples you
may consider placing a Glass Fiber (GF) pre-filter,
either integrated with the membrane as one unit
(Phenex-GF/NY or -GF/CA) or in series with the
membrane syringe filter of your choice.
15 mm Diameter
for 2 10 mL sample volumes
25 - 28 mm Diameter
for 10 100 mL sample volumes
Sample handling - FILTRATION
Application / Sample
HPLC and GC Sample Prep
Aggressive or Pure Organic Solvents
Protein Analysis / Biological Samples
High Particulate Loads
Environmental Methods
Food and Beverage
Clinical / Toxicology
Dissolution Testing
Ion Chromatography
Trace Metals (ICP-MS, AAS)
Capillary Electrophoresis (CE)
Tissue Cultures, Media, Buffers
Phenex-PES 3
(Polyethersulfone)
Phenex-PTFE 6
Phenex-NY
(Nylon)
Phenex-GF/NY 2
(Glass Fiber/Nylon)
Phenex-GF/CA 2,3,4
(Glass Fiber/Cellulose Acetate)
0.45 m
Phenex-RC
Phenex-PES 3
(Polyethersulfone)
Phenex-PTFE 6
Phenex-NY
(Nylon)
Part No.
Unit
Price
Part No.
Unit
Price
Part No.
Unit
AF0-3203-12
100/pk
AF0-2203-12
100/pk
AF0-8203-12
100/pk
AF0-3203-52
500/pk
AF0-2203-52
500/Pk
AF0-8203-52
500/pk
100/pk
AF0-8208-12
AF0-8208-52
AF0-3202-12
AF0-2202-12
100/pk
AF0-1202-12
100/pk
500/pk
100/pk
AF0-3202-52
500/pk
AF0-2202-52
500/pk
AF0-1202-52
500/pk
AF3-3207-12
100/pk
AF0-2207-12
100/pk
AF0-1207-12
100/pk
AF3-3207-52
500/pk
AF0-2207-52
500/pk
AF0-1207-52
500/pk
An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a Nylon
(NY) membrane. Excellent for filtration of particle-laden samples, such as foods and beverages,
environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the most
difficult samples. Outlet connection is luer-lock.
An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA
membrane. Excellent for filtration of tissue culture media, general biological sample filtration
and clarification. Outlet connection is luer lock.
AF0-1A47-12 7
100/pk
AF0-1A47-52 7
500/pk
AF0-8A09-12
100/pk
AF0-8A09-52
500/pk
AF0-3103-12
100/pk
AF0-2103-12
100/pk
AF0-8103-12
100/pk
AF0-3103-52
500/pk
AF0-2103-52
500/Pk
AF0-8103-52
500/pk
100/pk
AF0-8108-12
AF0-8108-52
AF0-3102-12
100/pk
AF0-2102-12
100/pk
AF0-1102-12
500/pk
100/pk
AF0-3102-52
500/pk
AF0-2102-52
500/pk
AF0-1102-52
500/pk
AF3-3107-12
100/pk
AF0-2107-12
100/pk
AF0-1107-12
100/pk
AF3-3107-52
500/pk
AF0-2107-52
500/pk
AF0-1107-52
500/pk
Phenex-GF/NY 2
(Glass Fiber/Nylon)
An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a Nylon AF0-1B47-12 7
(NY) membrane. Excellent for filtration of particle-laden samples, such as foods and beverages,
AF0-1B47-52 7
environmental, biofuels, and dissolution samples. Use less hand pressure to filter even the most
difficult samples. Outlet connection is luer-lock.
Phenex-GF/CA 2,3,4
(Glass Fiber/Cellulose Acetate)
An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA
membrane. Excellent for filtration of tissue culture media, general biological sample filtration
and clarification. Outlet connection is luer lock.
AF0-8B09-12
100/pk
AF0-8B09-52
500/pk
Prefiltration of heavily contaminated or highly viscous samples. When used in-series preceding a membrane filter, clogging of the membrane filter is prevented and sample clean up is
optimized. Outlet connection is luer lock.
AF0-8515-12
100/pk
AF0-8515-52
500/pk
1.20 m
Phenex-GF 2,3
(Glass Fiber)
Price
100/pk
500/pk
1. Larger quantity purchases at significant savings are available.
7. 28 mm diameter.
2. Glass fiber filters are 28 mm diameter and made of borosilicate. They will
remove 90 % of all particles >1.2 m.
8. Additional dimensions and membrane types are available. Please contact

your local Phenomenex technical consultant or distributor for availability or
assistance.
3. Housing material is methacrylate butadiene styrene (MBS) polymerisate.

Also known as Cyrolite.
4. Cellulose acetate is surfactant-free.
5. 26 mm diameter.
6. Hydrophobic membrane. Can be made hydrophilic by pre-wetting with IPA.
Phenomenex
Syringe Filters
Membrane Type/Size
0.20 m
Phenex-RC
Above syringe filters are non-sterile. Housing is made of

medical-grade polypropylene (PP), and offer luer lock inlet/slip
outlet connections, unless otherwise indicated.
11
Filtration
Sterile Syringe Filters
Centrifugal Filters
Sterile syringe filters from Phenomenex are ready-to-use, individually

blister packaged units, offering high flow rates at low inlet pressures,
for rapid sterile filtration.
Phenex
Disposable Centrifugal Filter

Convenient filtration of multiple HPLC and GC samples
Disposable Syringes / Sterile Syringe Filters / centrifugal filters
| FILTRATION - Sample handling
High recovery for small samples

Nylon, PTFE (Teflon) and Cellulose Acetate membrane
materials
Sterile Syringe Filters
Pore
Disc
Part No.
Size (m) Diameter (mm)
AF0-8455
0.2
28
AF0-8456
0.45
28
AF0-8457
0.2
28
AF0-8458
0.45
28
AF0-8459
0.2
25
AF0-8460
0.45
25
AF0-8461
0.2
25
Membrane
Material
CA Luer/Slip
CA Luer/Slip
PES Luer/Slip
PES Luer/Slip
RC Luer/Slip
RC Luer/Slip
PTFE Luer/Slip
Unit
50/pk
50/pk
50/pk
50/pk
50/pk
50/pk
50/pk
Price
Phenex disposable centrifugal filter units provide a convenient

means of filtering multiple HPLC and GC samples without the necessity of manually forcing each sample through the filter with a
syringe.
All-Plastic Disposable Syringes

Use for all syringe filter applications
Luer-lock outlet makes connection easy
Capacities ranging from 3 to 20 mL
Made of ultra-clean, high-purity plastics
All you need is a laboratory centrifuge with sufficient capacity for the
number of samples to be filtered at one time. Centrifugal force drives
the sample through the filter quickly without effort on the part of the
chemist. No cleaning of syringes is required between samples.
Samples contact only the Phenex filter unit itself and the polypropylene receiver tube. After filtration the upper portion of the tube
containing the membrane filter can be discarded. The receiver tube
serves as a container for the filtered sample and can be retained as
long as desired. Separate caps are supplied for the sample chamber
and the receiver tube.
Filter membrane materials available are Nylon 66, PTFE (Teflon)
and Cellulose Acetate (CA). Nylon 66 Phenex filter units are recommended for most HPLC and GC sample filtration work. They are
chemically resistant, compatible with all commonly used solvents,
and virtually free of extractable materials that could contaminate
samples. PTFE (Teflon) Phenex filters are designed to handle more
aggressive organic solvents, whereas Cellulose Acetate (CA) is
especially well-suited for the filtration of proteinaceous samples in
aqueous solvents.
Part No.
Description
AS0-8408 Plastic Disposable Syringes, Luer-lock
Capacity (mL)*
3
5
10
20
Unit
100/pk
100/pk
100/pk
100/pk
Price
Centrifugal Filter Units
Pore
Volumes (mL)
Membrane
Part No.
Size (m) Sample/Receiver Non-Sterile
AF0-0438
0.2
2.0 / 5.0
Nylon
AF0-0439
0.45
2.0 / 5.0
Nylon
AF0-0440
0.2
2.0 / 5.0
PTFE
AF0-0441
0.45
2.0 / 5.0
PTFE
AF0-8353
0.2
2.0 / 5.0
CA
AF0-8354
0.45
2.0 / 5.0
CA
Unit
25/pk
25/pk
25/pk
25/pk
25/pk
25/pk
Price
* Choose larger volume syringe to reduce force on syringe filter membrane. 10 mL syringe is recommended.
Above centrifugal filter units are non-sterile.
12
Phenomenex
Filtration
All-Glass Vacuum Mobile
Phase Filtration System
FilterSys
Prevents pump and system component damage
Rapid filtration of buffers, organics and corrosive liquids
Removes damaging microparticulates and bacterial
contaminants
HPLC and GC solvent and sample filtration, 1 to 4 L units
Solvent Pickup
Adapter
Phenex
Filter Membranes
Clamp
to Vacuum Line
Fritted Glass
Support Base
Filter Flask
WARNING: The apparatus should be

used with a water aspiration line, not a
true vacuum line, unless secured behind
an appropriate safety shield.
Mobile Phase Filtration System
Part No. Description
Complete Assembly
AH0-1566 FilterSys, 47 mm, 300 mL funnel with 1 L
vacuum flask
vacuum flask
vacuum flask
Component Parts
AH0-1567 Fritted support base, 47 mm, 40/35 taper
AH0-1568 Funnel, graduated, 300 mL, 47 mm
AH0-1569 1 liter filter flask, 40/35 taper
AH0-1570 Aluminum clamp, 47 mm
Filter Membranes
AF0-0503 Nylon 66, 0.2 m, 47 mm
AF0-0504 Nylon 66, 0.45 m, 47 mm
AF0-0513 PTFE, 0.2 m, 47 mm
AF0-0514 PTFE, 0.5 m, 47 mm
Unit
ea
ea
Price
Mobile Phase FILTRATION system
Compression
Fitting Connector
(to Solvent Source)
Graduated
Funnel
Valve
Recommended filter membranes: Nylon 66 is a highly resistant

material and can be used with almost all laboratory solvents.
Since Nylon 66 is hydrophilic, no prewetting of the filter is required.
PTFE (Teflon)membrane filters are excellent for organic or other
aggressive solvent systems. Extremely low levels of extractables
(plasticizers, contaminants, etc.) make this an excellent filter for
trace analysis work. PTFE is hydrophobic, so it is not recommended
for the filtration of aqueous solutions.
Mobile Phase - FILTRATION
Designed for rapid filtration of particulate matter from HPLC

solvents, this unit is an excellent value. Protect your instrument and
column from costly damage by clarifying all your HPLC solvents
and buffer solutions before use. This vacuum filter assembly comes
with a sample reservoir and receiving flask. A 47 mm diameter
membrane filter is placed between the fritted-glass support base
and the sample reservoir, secured in place by an aluminum clamp.
The support base itself is connected to the receiving flask by a
vacuum-tight ground-glass joint. Only low-extractable borosilicate
glass and the membrane filter come into contact with the mobile
phase. The vacuum hose connection is made above the filtration
drip tip to prevent contamination from the vacuum line.
ea
ea
ea
ea
ea
ea
ea
ea
ea
100/pk
100/pk
100/pk
100/pk
For compatible Solvent Pickup Adapter, see p. 14

For additional Filter Membranes, see p. 15
The following cross-reference chart will help you find the
right apparatus and components as compared with competing
products, and should save you time and money overall.
FilterSys Filtration Apparatus Cross-Reference Chart

Complete Assembly 47 mm, 300 mL
funnel with 1 L vacuum flask
Fritted Glass Support Base,
47 mm, 40/35 ST
Funnel, Graduated 300 mL, 47 mm
1 Liter Filter Flask, 40/35 ST
Aluminum Clamp, 47 mm
Solvent PIckup Adapter, 47 mm
Phenomenex
Alltech
Kontes
Rainin
Supelco
Waters
Whatman
Wheaton
AH0-1566
N/E*
953825-0000
419380
Z29,040-8
XX15 047 00
N/E*
419380
AH0-1567
N/E*
953826-0000
419382
Z29,060-2
XX15 047 02
N/E*
419382
AH0-1568
AH0-1569
AH0-1570
AH0-2947
350064
N/E*
350061
2343
953751-0000
953827-0000
953753-0000
953904-0000
419350
635525
419354
S-953906
Z29,052-1
Z29,061-0
Z29,056-4
Z29,059-9
XX10 047 04
XX15 047 05
XX10 047 03
N/E*
809C0010
N/E*
816C0045
N/E*
419350
635525
419354
419620
* N/E - No Equivalent Available.
Phenomenex
13
Solvent Reservoirs/Bottle
Filter Cap
Solvent Reservoir and Reagent

Bottles
Eliminates makeshift HPLC reservoir covers, such as

aluminum foil, Parafilm, etc.
Popular 1 and 2 liter sizes, equipped with 3-way valve cap
Neatly seals to prevent particulate contamination
Chemically inert, internal PTFE seal
Minimizes solvent evaporation and gas absorption
These wide-mouth GL45 mobile phase reservoirs come in the two

most popular 1- and 2-liter sizes. The reservoirs have easy-to-read
volumetric markings to indicate the amount of
solvent remaining. The versatile 3-way valve cap
supplied with each reservoir provides a totally-inert PTFE (Teflon) seal against the solvents inside.
A 2-way valve can be ordered separately.
Low-leaching (low alkali), borosilicate glass
A disposable filter (any size Luer lock Teflon or nylon syringe filter) on the pressure equalization port minimizes the reabsorption
of dissolved gases and prevents particle contamination. These
assemblies fit the standard screw cap bottles with 38 mm cap
size. The 1/8 in. OD Teflon feed line from
the cap attaches directly to the HPLC
pump. All cap parts are compatible with
most common HPLC solvents.
Mobile Phase accessories
FILTRATION - Mobile Phase
Filtration
Filter Cap
Part No.
Description
AH0-1565
Filter Reservoir Cap
Cap Size
38 mm
Unit
ea
Price
Reservoir and Valve Cap Assembly
Part No.
Mfr. No. Description
AH0-4142
3200
HPLC Reservoir, 1000 mL clear glass,
GL45 wide-mouth, includes 3-way
Valve Cap
AH0-4143
3203
HPLC Reservoir, 2000 mL clear glass,
GL45 wide-mouth, includes 3-way
Valve Cap
Unit
ea
Price
ea
Replacement Valve Caps
Solvent Pickup Adapter
For GL45 Neck Mobile Phase Reservoirs

Supplied with 1/8 in. fitted tubes, will not twist when
tightened
This glass adapter enables direct pickup of mobile phase solvent

for filtration using the Phenomenex FilterSys (see previous page).
Safe in-line filtration with the pickup adapter replaces the tedious
and dangerous pour-and-wait funnel filtration method.
Eliminate the possibilities of spilling solvents and
breathing toxic vapors. Replacing the funnel adapter
on the FilterSys unit, the 47 mm pickup adapter with
flange holds the membrane filter in place on top of
the fritted support base, which in turn is held by the
aluminum clamp (not included). The pickup adapter
draws solvent directly from the reagent bottle the
safest way to transfer and filter solvents. The adapter
includes a 4 mm PTFE (Teflon) valve with in. OD
outlet, PTFE 90 elbow with compression fittings
for in. OD tubing, and 3 feet of in. OD PTFE
tubing.
Two cap types (polypropylene outer cap or all-Teflon

block design)
Replacement 2- and 3-Valve Caps are available to fit the GL45threaded type of mobile phase reservoir offered on this page.
The valved caps provide an inert PTFE (Teflon) seal against the
solvents inside, and two types are available depending on construction of the outer cap: either polypropylene or all-Teflon block
design with stainless steel heli-coils to grip the Teflon tubing.
Select the polypropylene type if chloride salts or strong acids or
bases are being used. Valve caps are
pressure rated to 15 psi (1 bar) and are
autoclavable.
Replacement Valve Caps for GL45 Threaded Bottles*
Part No.
Description
AH0-8599
2-Way Valve Cap, GL45 threads, with polypropylene
outer cap
AH0-4141
3-Way Valve Cap, GL45 threads, solid PTFE (Teflon)
block construction, with tubing (1/8 in. OD)
Unit
ea
Price
Solvent Pickup Adapter
Part No.
Description
AH0-2947
Mobile Phase Pickup Adapter, 47 mm
Unit
ea
Price
ea
* Fittings not included. See p. 364 AQ0-2950
14
Phenomenex
Filtration
Additional Filtration Products
Filtration Products from Phenomenex
Regular sample filtration means:

Less system downtime
Removal of particulate matter to sub-micron levels is critical before any drug, tox, or dirty environmental sample is injected into an
HPLC, GC or mass spectrometer. Also, products that remove matrix
components, interferents, and chemical garbage will improve your
results. Check out the following useful products:

Mini-Index
Page No.
Filtration Products (General Laboratory)

Syringe filters. ............................................................... 10-12
Membrane filters and filtration apparatus.......................13
Centrifugal filters. ..............................................................12
Solid Phase Extraction (SPE) devices
Tubes and well plates............................................................35-56
Vacuum manifolds .................................................................57-59
Column Protection devices
SecurityGuard

UHPLC/HPLC (2.1 to 4.6 mm ID)..................................................239

Analytical (2 to 8 mm ID)..................................................... 235-239
SemiPrep (9 to 16 mm ID)................................................... 237-238
PREP (18 to 29 mm ID) . ..................................................... 237-238
PREP (30 to 49 mm ID) . ..................................................... 237-238
Inlet filters
Biocompatible PEEK .....................................................................16
Stainless steel . ............................................................................16
In-line filters
Biocompatible PEEK

Analytical .................................................................................17

SemiPrep .................................................................................18
Stainless steel

Analytical .................................................................................17
Prep . .......................................................................................18
Quality filtration and sample preparation products from Phenomenex offer a convenient, inexpensive way to clarify all your samples
for peace of mind and improved analytical results.
RC, Nylon, PTFE (Teflon), and other membranes available

Wide selection of membrane sizes
Phenex PTFE (Teflon) and Regenerated
Cellulose (RC) membrane filters offer excellent
chemical resistance to almost all laboratory
solvents and samples. They do not introduce
unwanted plasticizers or extractables into the
sample or mobile phase. Since Regenerated
Cellulose (RC) is hydrophilic, filtering of aqueous
solvents is simple. No prewetting is required.
PTFE is hydrophobic and so is not recommended
for the filtration of aqueous solutions.
Filter Membranes
Pore
Disc
Part No.
Size (m) Diameter (mm)
Nylon
AF0-0500
0.45
13
AF0-0501
0.2
25
AF0-0502
0.45
25
AF0-0503
0.2
47
AF0-0504
0.45
47
AF0-0505
0.2
90
AF0-0506
0.45
90
PTFE
AF0-0509
0.2
13
AF0-0511
0.2
25
AF0-0512
0.45
25
AF0-0513
0.2
47
AF0-0514
0.45
47
Cellulose Acetate (CA)
AF0-8434
0.45
13
AF0-8435
0.2
13
AF0-8436
0.45
25
AF0-8437
0.2
25
AF0-8438
0.45
47
AF0-8439
0.2
47
Regenerated Cellulose (RC)
AF0-8440
0.45
13
AF0-8441
0.2
13
AF0-8442
0.2
25
AF0-8443
0.45
47
AF0-8444
0.2
47
Polyethersulfone (PES)
AF0-8445
0.2
25
AF0-8446
0.45
25
AF0-8447
0.2
47
AF0-8448
0.45
47
Cellulose Nitrate Ester (MCE)*
AF0-8449
0.80
13
AF0-8450
0.45
13
AF0-8451
0.80
25
AF0-8452
0.45
25
AF0-8453
0.80
47
AF0-8454
0.45
47
Membrane
Material
Unit
Nylon
Nylon
Nylon
Nylon
Nylon
Nylon
Nylon
100/pk
100/pk
100/pk
100/pk
100/pk
5/pk
5/pk
PTFE
PTFE
PTFE
PTFE
PTFE
100/pk
100/pk
100/pk
100/pk
100/pk
CA
CA
CA
CA
CA
CA
100/pk
100/pk
100/pk
100/pk
100/pk
100/pk
RC
RC
RC
RC
RC
100/pk
100/pk
100/pk
100/pk
100/pk
PES
PES
PES
PES
100/pk
100/pk
100/pk
100/pk
MCE
MCE
MCE
MCE
MCE
MCE
100/pk
100/pk
100/pk
100/pk
100/pk
100/pk
Price
| Filter Membranes / additional products
Fewer troubleshooting problems
Phenex
FILTRATION
Filtration is the easiest way to improve your results, guard your

system components from damage, and protect your column investment.
Filter Membranes
*MCE = Mixed Cellulose Esters

Above filter membranes are non-sterile.
Phenex is a trademark of Phenomenex, Inc.
For a useful Membrane Selection Guide, see p. 10
Phenomenex
15
Filtration
Stainless Steel
Metal-Free/Biocompatible
Protects pumps and check valves
Biocompatible
Easy to replace
Flat bottom design uses all available mobile phase
Low cost
High surface area for long filter life
HPLC solvent inlet filters are used at the low pressure inlet side of
the pump to help protect the check valves, injector and column
from damaging particulate contamination. Solvent filters are constructed of Hastelloy Steel and are available for 1/16 in. ID and 1/8 in.
ID tubing. Due to the large surface area of the cylindrical frit, virtually no backpressure or cavitation is developed. The filter is easily
cleaned by backflushing or sonicating.
The Solvent Saver Inlet Filter Unit utilizes a flat filter element that
sits parallel to the bottom of the HPLC reservoir. The design allows
the filter to draw all but the last 2 % of the mobile phase from the
reservoir without drawing air into the system. Compare this with
conventional cylindrical mobile phase filters that begin to draw air
into the system when less than 10 % of the solvent remains in the
reservoir.
The Solvent Saver Inlet Filter is manufactured by pressing a PEEK
filter element into an inert Teflon housing. The top of the housing has female 1/4 in.-28 threads to accept 1/8 or 1/16 in. OD tubing
via PEEK flangeless fittings and Tefzel ferrule (sold separately) or
direct connect various size tubing using the Unifit adapter (sold
separately). The Unifit adapter slip-fits onto 1.5, 2.2 or 3.0 mm ID
tubing. This filter is excellent for sensitive biochromatography and
ion chromatography applications where metal surfaces may corrode or interact with samples.
InLet Filters
FILTRATION - Mobile Phase
Inlet Filters
Pump Inlet Filter

Unifit adapter
(sold separately AQ0-8339)
Solvent Inlet Filters - Stainless Steel
Part No.
Description
AF0-0356 Solvent Inlet Filter, 2 m, for 1/16 in. ID tubing
AF0-0359 Solvent Inlet Filter, 2 m, for 1/8 in. ID tubing
AT0-2955 Teflon Tubing, 5 ft. L x 1/8 in. OD x 1/16 in. (0.062 in.) ID
AT0-2956 Teflon Tubing, 10 ft. L x 1/8 in. OD x 1/16 in. (0.062 in). ID
AT0-8610 Teflon Tubing, 10 ft. L x 1/4 in. OD x 1/8 in. (0.125 in). ID
slip-fits onto 1.5,

2.2 or 3.0 mm ID
tubing
Unit
ea
ea
ea
ea
ea
ea
Price
Important: Depending on the mobile phase, we recommend

that you change your inlet filter every one to six months.
For disposable Syringe Filters offering convenient and
economical sample and solvent filtration, see pp. 10-12
Solvent Saver Inlet Filter - Metal-Free
Part No.
Description
AH0-1562 Solvent Saver Inlet Filter with 10 m PEEK
filter with Flangeless fitting for 1/8 in. OD tubing
AQ0-8339
AQ0-2949
AQ0-2950
AT0-2953
AT0-2955
AT0-2956
AT0-8610
16
1/4 in.-28 flangeless

fitting (included) attaches
to 1/8 in. OD tubing
Solvent Saver Unifit Adapter, Tri-Step Tubing Connector,

PEEK
Flangeless Nut and Ferrule for 1/16 in. OD tubing,
in.-28 threads, red Delrin
Flangeless Nut and Ferrule for 1/8 in. OD tubing,
in.-28 threads, green Delrin
Teflon Tubing, 5 ft. L x 1/16 in. OD x 1/32 in. (0.031 in.) ID
Teflon Tubing, 5 ft. L x 1/8 in. OD x 1/16 in. (0.062 in.) ID
Teflon Tubing, 10 ft. L x 1/8 in. OD x 1/16 in. (0.062 in). ID
Teflon Tubing, 10 ft. L x 1/4 in. OD x 1/8 in. (0.125 in). ID
Unit
ea
Price
ea
10/pk
10/pk
ea
ea
ea
ea
Phenomenex
Filtration
In-Line Filters
best seller
Stainless Steel (Analytical)
KrudKatcher
Removes particulates from flow path
Universal fit to virtually all manufacturers analytical

columns
Minimizes sample peak dispersion
Unit
ea
5/pk
ea
5/pk
Price
Biocompatible
Effective pre-column filtration
Virtually no band broadening or peak distortion
Easy fingertight connection
This in-line filter assembly removes fine particles from the solvent
stream without adding band broadening or peak distortion to your
separation. Microparticulates down to 0.5 m are effectively removed before they have a chance to plug your column and degrade
your separation. The Polyglas frit with fluoropolymer frit assembly
design is fully biocompatible and easily installed using fingertight
connections.
Whereas conventional in-line lters typically cost much more and

often require replacement parts and tools, the KrudKatcher is a
simple, low-cost unit that is easily replaced and discarded when
the backpressure indicates clogging or excessive particle build up.
No replacement parts are required just unscrew, throw away and
replace with a new lter in seconds. The universal connection is
compatible with all standard 1/16 in. 10-32 internal threaded endttings used in columns, guard columns, injector valves, and other
LC equipment.
The KrudKatcher Classic:

Pressure-rated to 5,000 psi (345 bar)
The PEEK lter body of the KrudKatcher Classic houses an
integrated 0.5 m 316 stainless steel depth lter that efficiently
removes microparticulates from the ow stream with minimal contributions to system dead volume (2 L).
KrudKatcher Classic Disposable In-Line Filter
Part No.
Description
AF0-5727 KrudKatcher Disposable Pre-Column
Filter, 0.5 m
Unit
10/pk
Price
The KrudKatcher Ultra:

Fits virtually all UHPLC / HPLC columns
1.0 to 4.6 mm ID
Pressure rated to 20,000 psi (1,375 bar)
Extremely low dead volume minimizes sample peak dispersion
The KrudKatcher Ultra filter body houses an integrated 0.5 m
316 stainless steel depth filter that efficiently removes microparticulates from the flow stream without contributing to system
backpressure or dead volume (<0.2 L).
KrudKatcher Ultra In-Line Filter
Part No.
Description
AF0-8497 HPLC Krudkatcher Ultra Column In-Line Filter, 0.5
m Depth Filter x 0.004 in. ID
Unit
3/pk
Price
KrudKatcher Ultra requires 5/16 in. wrench. Wrench not provided. See p. 373
Unit
ea
ea
For SecurityGuard,
the universal guard cartridge system, see p. 235
Phenomenex
There are two types of KrudKatchers depending on system backpressure and dead volume demands: the KrudKatcher Classic and
the KrudKatcher Ultra.
Hand-tightened connection
Metal-Free/Biocompatible (Analytical)
Metal-Free/Biocompatible In-Line Filter (Analytical)
Part No.
Description
AF0-1736 In-line Filter, 0.5 m Frit
AF0-1737 Replacement Filter Frit Assembly, 0.5 m
Convenient, disposable in-line lter
| in-line Filters
Stainless Steel In-Line Filters (Analytical)
Part No.
Description
AF0-0375 In-line Filter with 0.5 m Porosity x 1.5 mm dia. filter
AF0-0376 Replacement Filter Disks: 0.5 m x 1.5 mm
AF0-0377 In-line Filter with 0.5 m Porosity x 3 mm dia. filter
AF0-0378 Replacement Filter Disks: 0.5 m x 3 mm
Saves expensive columns and equipment from damaging

microparticulates
FILTRATION
Two column In-line Filters are available

to protect expensive HPLC columns
from damaging microparticulates.
Using one of these filters between the
injection valve and the column is recommended for all HPLC systems. The 3 mm diameter
filter element is recommended for use with conventional
4.6 mm diameter columns. The 1.5 mm diameter filter is
recommended for use with smaller diameter columns, microbore
columns, and very short columns for fast HPLC procedures. The
1.5 mm version provides minimal dispersion for this type of filter,
but has only about the sample load capacity of the 3 mm version.
Both Column In-line Filters are supplied with two 6 cm L x 0.007 in.
ID connecting tubes. Pressure rating of both models is 7000 psi.
Price
If KrudKatcher in-line filters do not perform as well

or better than your current in-line filter product of
similar size, return the product with comparative
data within 45 days for a FULL REFUND.
17
in-line filters / COLUMN COUPLERS
| FILTRATION
Filtration
In-Line Filters (contd)
Analytical Column Couplers
Metal-Free/Biocompatible (SemiPrep)
Sure-Lok Coupler
For columns 8 to 18 mm ID
Universal and reusable
Effective pre-column filtration
Solvent resistant material
Replaceable filter element
Low dead-volume connection
Phenomenexs Biocompatible SemiPrep in-line filter holder with

replaceable filter element (2 m PEEK frit) will help protect your
column investment by safely removing particulate matter and insoluble material from the mobile phase and sample matrix. The
filter consists of a stainless steel body, two PEEK end-fittings, and
a separate PEEK frit . When you need to replace the filter, simply
unscrew the assembly, remove the frit and replace it. This filter unit
can be placed in the flow path before or after the column with little
or no effect on peak shape. Pressure rated to 6,000 psi.
Compatible with all 10-32 internal-threaded fittings
Metal-Free/Biocompatible In-Line Filter (SemiPrep)
Part No.
Description
AF0-8420 HPLC SemiPrep Column In-Line Filter
2.0 m Porosity x 10 mm dia. filter, Biocompatible
AF0-8428 Replacement In-Line Filter Disk, PEEK,
2.0 m Porosity x 10 mm dia.
Unit
ea
Price
Applications:
Filter to column
Column to column
Precolumn to column
Column to detector
Sure-Lok Coupler (PEEK)
Sure-Lok Couplers contain two Sure-Lok male nuts at either end

of a 5 cm long 1/16 in. tubing. The PEEK biocompatible coupler
has all parts composed of PEEK, including the 0.010 in. ID tubing.
Fingertight to 5,000 psi (345 bar).
Sure-Lok Couplers
Part No.
Description
AQ0-1392
PEEK Sure-Lok Coupler
AQ0-1393
5/pk
Stainless Steel (PREP)

Economical protection for preparative HPLC columns
and injectors
Unit
ea
10/pk
Price
Unit
ea
Price
PREP Column Coupler
For columns 19 to 30 mm ID
Replaceable filter element
Preparative columns and the HPLC systems on which they are used
are costly and must be protected against fouling. Phenomenexs
PREP In-line Filter holder with replaceable filter element (2 m stainless steel frit) will help protect your investment by safely removing
particulate matter and insoluble material from the mobile phase and
sample matrix. The filter unit can be placed in the flow path before
or after the column with little or no
effect on peak shape. This versatile
filter can also protect check valves,
injectors and detectors. Pressure
rated to 8,000 psi (551 bar).
Stainless Steel In-Line Filters (PREP)
Part No.
Description
AF0-7866
HPLC PREP Column In-line Filter, S.S.,
2.0 m Porosity x 21.2 mm dia.
AF0-7867
Replacement In-Line Filter Disks, S.S.,
AQ0-7877
PREP Replacement O-Rings, 1 in. OD x
7/8 in. ID x 1/16 in. CS, Fluorocarbon
AT0-0465
Capillary S.S. Tubing, 0.020 in. ID x 0.062 in.
(1/16 in.) OD x 10 cm L
AT0-0466
Capillary S.S. Tubing, 0.020 in. ID x 0.062 in.
(1/16 in.) OD x 20 cm L
Unit
ea
PREP Column Coupler
Part No.
Description
AQ0-8374
PREP Coupler, Stainless Steel with PEEK
Ferrule Inserts, 1/16 in. OD x 0.020 in. ID
AQ0-8375
Replacement Ferrule Inserts for PREP Coupler,
PEEK 0.020 in. ID
10/pk
Price
5/pk
2/pk
5/pk
5/pk
For SecurityGuard,
the universal guard cartridge system, see p. 235
18
Phenomenex
Sample Handling
Pipettes
Pipettes and Pipet Tips
19-20
Pipettes
Adjustable Pipettes
Ensure Accurate and Precise Measurement
Calibration report provided (ISO 8655-6)
Universal Finger Rest
ISO 9001 : 2000 certified
comfort for both right or left

handed users
3-year factory warranty
Soft Hand Grip
made from thermoplastic

elastomer to reduce fatigue
and stress
Pipettes - Sample handling
Adjustable volumes, each color coded
Large Display
ensures accurate volume settings
Narrow Neck Shaft
pipettes & pipet tips
Soft Ergonomic Plunger

shaped to reach down into
narrow tubes
with reduced resistance for

effortless pipetting
Quick Ejector
smooth action reduces sample
contamination
Lightweight Body
constructed from light weight
polymers to reduce fatigue
Resistant Tip
designed to resist shock
and chemical corrosion
Pipettes
best seller
Pipettors - Orbit Precision Adjustable Pipette

Part No.
Vol. Range
Unit
Price
0PVA-025 0.1-2.5 L
ea
0PVA-10
0.5-10.0 L
ea
0PVA-20
2.0-20.0 L
ea
0PVA-50
5.0-50 L
ea
0PVA-100 10-100 L
ea
0PVA-200 20-200 L
ea
0PVA-1000 100-1000 L
ea
0PVA-5000 500-5000 L
ea
Orbit Micro Pipette Stand
0PST-004 Holds 4 pipettes
ea
Pipet Tips
Matching Tips
Phenex Non-Sterile Pipet Tips in Racks
Part No.
Vol. Range
AH0-5918
0.5-10.0 L
AH0-5919
0.5-10.0 L
AH0-5919
0.5-10.0 L
AH0-5917
5-200 L
AH0-5917
5-200 L
AH0-5917
5-200 L
AH0-5920
100-1000 L
AH0-8488*
100-5000 L
*packaged in bag
20
Qty
960
960
960
960
960
960
1000
500
Price
Matching Tips
Phenex Sterile Pipet Tips in Racks
Part No.
Vol. Range
AH0-5922
0.5-10.0 L
AH0-5923
0.5-10.0 L
AH0-5923
0.5-10.0 L
AH0-5921
0.5-10.0 L
AH0-5921
5-200 L
AH0-5921
5-200 L
AH0-5924
100-1000 L
AH0-8489
100-5000 L
Qty
960
960
960
960
960
960
1000
120
Price
Pipet Tips Compatibility Chart for

Orbit Precision Adjustable Pipettes
Pipette Part No.
0PVA-025
0PVA-10
0PVA-20
0PVA-50
0PVA-100
0PVA-200
0PVA-1000
0PVA-5000
Phenomenex
Sample Handling
Vials
vials
Sample Vials
Autosampler Vials, Caps, Septa, and Inserts
Verex Vials Quality and Certification.............................................. 22-23
8 mm................................................................................................... 30
9 mm........................................................................................ 24, 27-29
10 mm........................................................................................... 24, 31
11 mm............................................................................................ 24-27
13 mm................................................................................................. 32

Specialty, 12 x 32 mm......................................................................... 24
Shell Vials................................................................................................ 32
Headspace Vials...................................................................................... 33
VOA / ASE Vial Kits.................................................................................. 33
Storage Vial Kits...................................................................................... 33
Autosampler Compatibility Chart............................................................ 34
21 - 34
Vials
for HPLC/ GC /SFC and MS Analysis

Certified Vial Products
Verex
Leading in Quality, Delivery and Support
The most critical point of your analysis is when you put your sample
in the vial. Verex certified sample vials, inserts, caps and seals are
guaranteed to ensure problem-free, reproducible performance with
consistent results you can trust.
All Vials, Caps and Kits

Advanced manufacturing
Multi-step QA/QC
Cleanroom packaged
AUTOSAMPLER VIALS | VIALS - Sample handling
Certified
With the demand for better product reproducibility and performance
in glass, septa and caps, we developed Verex HPLC / GC vial
products with high quality materials to exceed industry specifications
and tolerances. Verex innovative precision products provide airtight, leak-free seals to safely transfer and store your most important
samples. Through extensive testing, weve created guaranteed-fit
vial products that offer compatibility with virtually any autosampler
for trouble-free operation.
Vials and inserts are made of inert, high-purity, chemically-stable

borosilicate glass to exacting specifications, individually heattreated to burn off any impurities, then packaged in a cleanroom
environment. Choose from crimp, snap, and screw types or mix and
match vials and caps to your application. Most vials are offered with
or without a write-on marking spot or patch. For easy selection,
purchase convenience, or assembled vial kits ready to go!
Verex caps / seals / closures are made to exacting specifications,
giving a tight seal each and every time. The aluminum crimp seals,
plastic screw and snap caps come with highly pure septa material
and are available in pre-slit and non-slit formats. Economical press-fit
and specially formulated bonded-in septa styles are both available.
And our NEW mass spec certified (MSQ) screw caps provide you
with a state-of-the art solution for your most challenging, sensitive
applications.
A variety of complete vial kits are available to meet the most
demanding needs of chromatographers, from routine analytical to
high-sensitivity work:
33 Expansion clear glass (USP Type 1 borosilicate,
Class A), 51A amber glass
With tightly controlled 100 % traceable manufacturing processes,

we re-defined the standards for lot traceability and certification of
every Verex product to ensure a uniform product with optimum
performance for any application. For those with the most sensitive
applications, choose Verex-Certified PLUS or certified MSQ
products for excellent ultra-grade cleanliness. Bottom line, Verex is
your sample transfer and storage solution.
Limited volume (high sample recovery) vials
Three Levels of Certification
Certified
Certified + PLUS
(Cert+)
Certified MSQ
(Cert + MSQ)
(Mass Spec Quality)
Silanized (deactivated) glass

Polypropylene for biocompatibility
Certified and special MS-certified (caps and vials)
Ultra Clean
Specially designed and treated for mass spectrometry (MS) applications.
Low Bleed
Special cap / closure processing, treating, and testing for low bleed or residual
organics. For high sensitivity GC/MS and LC/MS applications.
Dimensionally Verified
Machine vision cameras and gauges inspect critical dimensions and tolerances
to produce uniform, defect-free products.
Lot Traceable
Vial and cap packs are 100% traceable through the manufacturing process.
Certificate of Conformance available by lot number upon request.
Clean Room Packaged

Ensures particulate- and contaminate-free products for clean chromatography.
No Extension Needed
Certificate of Conformance
Included;
Extension -C
Certificate of Conformance
Included;
Extension -M
AR0-9921-13
AR0-9921-13-C
AR0-9921-13-M
Part Number Extension
Part Number Example
Need help matching your current

vials and caps to Verex?
Visit www.phenomenex.com/VialMatch
22
Phenomenex
Vials
for HPLC/ GC /SFC and MS Analysis

If Verex vials and caps do not perform
as well or better than your current
vial and cap products of similar type,
dimensions, and material, return the
product with comparative data within
45 days for a FULL REFUND.
All Verex products are fully lot-tested and certified. The

details of product specifications and testing are available at
www.phenomenex.com/verex. The Certified PLUS rating signifies
the highest quality available from Phenomenex. A Certificate of
Conformance is included. Depending on the product, Certified
PLUS may indicate, for example, special processing or treating
and /or testing for low bleed or residual organics. Certified products
offer excellent performance, especially for high sensitivity, mass
spectrometry applications.
RIGHT CHOICE
From the manufacturing of our products to their timely delivery

and superior customer support, we are dedicated to continually
improving our processes to consistently meet or exceed your
expectations.
The Most Critical Part of Your Analysis!

Though they may look alike, not all vials offer equivalent performance. Variations in product quality can adversely affect your
chromatography, leading to mystery peaks, loss of analytes, and
Verex Products:
Improved analytical results
Fewer contaminants
Smoother baselines
Soak Blank
100
irreproducible results. Begin every analysis with high quality Verex

products to minimize troubleshooting delays, and costly, unnecessary rework.
Sample handling - VIALS | AUTOSAMPLER VIALS
Certified Plus
MS Quality
Scan ES+
5.98e9
Verex MSQ Certified Kit
Level 3
Scan ES+
5.98e9
100
Competing Products Risk:

Mystery / ghost peaks
Increased interferences
Inaccurate results
Company A
Certified LC/MS Kit
100
Scan ES+
5.98e9
Company B
LC/MS Certified Kit
Scan ES+
5.98e9
Company C
LC/MS Certified Pre-Cleaned Kit
Scan ES+
5.98e9
100
100
0
100
150
200
250
300
350
400
450
500
550
600
650
700
750
800
850
900
950
m/z
1000
Comparative separations may not be representative of all applications.
Phenomenex
23
Inserts for 9-425 Screw-Tops Vials
Snap to Vials, Specialty
Verex Vials
Inserts
Inserts
for 9-425
for 9-425
Screw-Tops
Screw-Tops
VialsVials
12 x 32 mm Specialty Vials

Inserts for 9-425 Screw-Tops
Vials
Inserts for
9-425 Screw-Tops Vials
12 x 32mm Specialty Vials
Type and Description
High-Recovery
CD Vial
SnapSnap
to Vials,
to Vials,
Specialty
Specialty
s for 9-425 Screw-Tops Vials
Material
Total Volume
Residual Volume
Available as
Page Number
9-425 Screw Thread
Glass
1.5mL
< 20L
Convenience
Kits
(certified
and regular)
28
28
27
27
AR0-9981-13
AR0-9982-13
AR0-9985-13-C
AR0-9986-13-C
9-425 Screw Thread
Glass
1.5mL
< 2L
Convenience
Kits
(certified)
27
27
AR0-9987-13-C
AR0-9988-13-C
11mm Snap
Glass
300L
< 4L
Vials
(regular)
26
26
AR0-3630-13
AR0-3631-13
< 2L
Convenience
Kits
(certified and
regular)
26
26
28
28
27
27
27
AR0-9671-13
AR0-9672-13
AR0-9973-13
AR0-9974-13
AR0-9974-13-C
AR0-9978-13-C
AR0-9979-13-C
26
25
25
28
28
AR0-3625-13
AR0-3725-13
AR0-3726-13
AR0-3920-13
AR0-3921-13
Center-draining
Part Numbers
w-Tops Vials
Max Recovery
CD Vial
Center-draining
Insert Vial
o Vials, Specialty
Finish
Vial i2V
12 x12
32mm
x 32mm
Specialty
Specialty
VialsVials
Insert Vial
Vial i3 (Qsert)
11mm Snap or
9-425 Screw Thread
Glass
475L
12 x 32mm Specialty12
Vials
x 32mm Specialty Vials
Insert Vial
Vial i3 (Qsert)
11mm Crimp or
11 mm Snap or
9-425 Screw Thread
Glass
475L
< 2L
Vials
(regular)
10-425 Screw Thread
Glass
450L
< 2L
Vials
(regular)
31
31
AR0-3020-13
AR0-3021-13
9-425 Screw Thread
Polypropylene
700L
< 5L
Convenience
Kits
(certified)
27
27
AR0-9993-13-C
AR0-9994-13-C
9-425 Screw Thread
Polypropylene
300L
< 2L
Convenience
Kits
(certified and
regular)
28
28
27
AR0-9991-13
AR0-9992-13
AR0-9995-13-C
Vials
(regular)
31
26
26
25
25
28
28
AR0-3040-13
AR0-3640-13
AR0-3641-13
AR0-3740-13
AR0-3741-13
AR0-3940-13
AR0-3941-13
2mm Specialty Vials
Insert Vial
Vial i3 (Qsert)
Vials
Plastic Vial
Plastic Vial
11 mm Crimp or
Micro Vial with Tapered Base
11mm Snap or
9-425 Screw Thread
v-Vial
or 10-425mm Screw
Thread
Glass
1.4mL
< 4L
For example, 9-425 neck finish represents a vial with a

diameter of 9 mm across the outside of the threads and
a thread style of 425.
24
Phenomenex
Verex Vials
12 x 32 mm, 11 mm Crimp-Top Vial Products

Crimp-Top Vials, 2.0 mL
Cleaner vials eliminate ghost peaks and contaminants
Used with most autosamplers, including Agilent, Thermo
Scientific, Waters
Larger-opening wide-mouth style prevents broken
needles and system downtime
Precision neck improves crimping
1000/pk
Price
AR0-3700-13
AR0-3710-13
AR0-3711-13
AR0-37K0-13
AR0-37L0-13
AR0-37K1-13
AR0-37L1-13
Specialty Crimp-Top Vials

Microsampling (center-draining and fused-insert vials)
Description
Vial, Crimp, v-Vial Clear, No Patch*
Vial, Crimp, v-Vial Amber, No Patch*
Vial, Crimp, Vial i3 (Qsert), Clear, w/ Patch
Vial, Crimp, Vial i3 (Qsert), Amber, w/ Patch
100/pk
AR0-3725-12
AR0-3726-12
Price
1000/pk
AR0-3740-13
AR0-3741-13
AR0-3725-13
AR0-3726-13
Description
Standard Opening
Vial, Crimp, 2 mL Clear, No Patch
Vial, Crimp, 2 mL Clear, w/ Patch
Vial, Crimp, 2 mL Amber, w/ Patch
Wide Mouth Opening
Vial, Crimp, 2 mL Wide Mouth, Clear, No Patch
Vial, Crimp, 2 mL Wide Mouth, Clear, w/ Patch
Vial, Crimp, 2 mL Wide Mouth, Amber, No Patch
Vial, Crimp, 2 mL Wide Mouth, Amber, w/ Patch
Price
*51-Expansion glass.
Seals / Closures
for
12 x 32mm Specialty
Vials Crimp-Top Vials
Excellent for volatile samples

Extra clean to eliminate contamination
Colored aluminum
Description
Seal, 11mm Diameter, Crimp, PTFE/Silicone, silver
Seal, 11mm Diameter, Crimp, PTFE/Silicone/PTFE, silver
Seal, 11mm Diameter, Crimp, PTFE/Rubber, silver
Seal, 11mm Diameter, Crimp, PTFE/Rubber, blue
Seal, 11mm Diameter, Crimp, PTFE/Rubber, red
Seal, 11mm Diameter, Crimp, PTFE/Rubber, green
Seal, 11mm Diameter, Crimp, PTFE/Rubber, gold
Seal, 11mm Diameter, Crimp, PTFE, silver
Phenomenex
1000/pk
AR0-5780-13
AR0-5760-13
AR0-5740-13
AR0-5742-13
AR0-5741-13
AR0-5743-13
AR0-574G-13
AR0-5710-13
Price
Save Money. Bulk discounts available.

Timely delivery. Excellent support.
Request a Quote. Want to make sure

youre getting the best pricing on vials?
Visit Phenomenex.com/Verex
25
Verex Vials
12 x 32 mm, 11 mm Snap-Top Vials and Kits

Convenience Kits Snap-Top Vials and Seals / Closures
Precision formed for autosampler fit

Larger-opening wide-mouth style prevents broken needles
and system downtime
Rigorous quality testing for consistent performance
Precise vial depth provides consistent sample recoveries
Description
Vial Kit, Snap Cap, 2 mL Clear, w/ Patch + PTFE/Silicone
Vial Kit, Snap Cap, 2 mL Clear + PTFE/Silicone, preSlit
1000/pk
AR0-9721-13
AR0-9727-13
Price
No write-on patch unless otherwise indicated
Convenience Kits Specialty Snap-Top Vials and Seals / Closures

Microsampling (fused-insert vials)
Cleaner vials eliminate ghost peaks and contaminants
Description
Vial Kit, Snap, Vial i3 (Qsert), Clear w/ Patch + PTFE/Silicone
Vial Kit, Snap, Vial i3 (Qsert), Clear w/ Patch + PTFE/Silicone, preSlit
100/pk
AR0-9671-12
AR0-9672-12
Price
1000/pk
AR0-9671-13
AR0-9672-13
Price
Snap-Top Vials*, 2.0mL

Great for less volatile samples or high-throughput labs
Save time, without crimping or twisting

Description
Vial, Snap, 2mL Clear, No Patch
Vial, Snap, 2mL Clear, w/ Patch
Vial, Snap, 2mL Amber, w/ Patch
Vial, Snap, 2mL Clear, w/ Patch, Silanized
Vial, Snap, 2mL Amber, w/ Patch, Silanized
1000/pk
AR0-3600-13
AR0-3610-13
AR0-3611-13
AR0-3613-13
AR0-3614-13
Price
*Vials can accept either snap or crimp-tops to effectively seal the vial.
Snap-Top Vials, Specialty
For small volume microsamples (center-draining and fused-insert vials)
One piece vial with fused-insert

Description
Vial, Snap, v-Vial i2V Clear, No Patch
Vial, Snap, v-Vial i2V Amber, No Patch
Vial, Snap, Vial i2V Clear, No Patch
Vial, Snap, Vial i2V Amber, No Patch
Vial, Snap, Vial i3 (Qsert), Clear, w/ Patch
100/pk
AR0-3640-12
AR0-3641-12
AR0-3630-12
AR0-3631-12
AR0-3625-12
Vials, Specialty Price

Price Snap to1000/pk
AR0-3640-13
AR0-3641-13
AR0-3630-13
AR0-3631-13
AR0-3625-13
51-Expansion glass.
Seals / Closures for Snap-Top Vials

Specially designed for secure fit
Easily snap cap onto vial and pull cap off
Description
Seal, 11mm Diameter, Snap, PTFE/Silicone, blue
Seal, 11mm Diameter, Snap, PTFE/Silicone, natural
Seal, 11mm Diameter, Snap, PTFE/Silicone preSlit, blue
Seal, 11mm Diameter, Snap, PTFE/Silicone preSlit, natural
Seal, 11mm Diameter, Snap, PTFE/Rubber, natural
Seal, 11mm Diameter, Snap, PTFE, blue
26
1000/pk
AR0-5652-13
AR0-5656-13
AR0-5672-13
AR0-5676-13
AR0-5646-13
AR0-5612-13
Price
Simply choose the vial closure convenience

kit for your application and youll also receive
a free dispenser box!
Phenomenex

Vials Screw-Tops Vials
Inserts for 9-425
Inserts for 9-425 Screw-Top
Verex Vials

12 x 32 mm, 9-425 (9 mm)
Screw-Top Vials and
Kits
Convenience Kits Certified PLUS (Cert+) Mass Spec Quality (MSQ)

9-425 Screw-Top Vials and Caps with Locked-Fit Septa
For your most demanding analysis; high sensitive detectors
HPLC and LC/MS tested and certified
RIGHT CHOICE
State-of-the-art design and manufacture
Description
Cert+ MSQ Vial Kit, 9 mm, 2 mL Clear w/ Patch + MSQ PTFE/Silicone
Cert+ MSQ Vial Kit, 9 mm, 2 mL Amber w/ Patch + MSQ PTFE/Silicone
1000/pk
AR0-992A-13-M
AR0-992B-13-M

Price
Certified Plus
MS Quality
Convenience Kits Certified PLUS (Cert+) 9-425 Screw-Top Vials

and Caps with Bonded-In Septa
Polyethylene-Starburst cap is an excellent choice for inertness
Description
Cert+ Vial Kit, 9 mm, 2 mL Clear w/ Patch + PTFE/Silicone cap
Cert+ Vial Kit, 9 mm, 2 mL Clear w/ Patch + PE-Starburst cap
Cert+ Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Silicone, preSlit cap
Cert+ Vial Kit, 9 mm, 2 mL Amber w/ Patch + PE-Starburst cap
1000/pk
AR0-9921-13-C
AR0-9927-13-C
AR0-9926-13-C
AR0-9928-13-C
2
Price
Convenience Kits Certified PLUS (Cert+) Specialty 9-425 Screw-Top Vials and Caps
Kits for microsampling (center-draining and fused-insert vials)
Lower trace contaminants for higher sensitivity work
12 x 32mm Specialty
12 Vials
Inert polypropylene (PP) vials for biochromatography, or pH-sensitive applications

Ordering Information*
Description
100/pk
Cert+ Vial Kit, 9 mm, Maximum Recovery (CD) Clear + PTFE/Silicone cap
AR0-9987-12-C
Cert+ Vial Kit, 9 mm, Maximum Recovery (CD) Clear + PTFE/Silicone, preSlit cap AR0-9988-12-C
Cert+ Vial Kit, 9 mm, High Recovery (CD) Clear + PE-Starburst cap
AR0-9985-12-C
Cert+ Vial Kit, 9 mm, High Recovery (CD) Amber + PE-Starburst cap12 x 32mm Specialty
AR0-9986-12-C
Vials
Cert+ Vial Kit, 9 mm, Vial i3(Qsert) Clear w/ Patch + PTFE/Silicone, preSlit cap
AR0-9974-12-C
Cert+ Vial Kit, 9 mm, Vial i3(Qsert) Clear w/ Patch + PE-Starburst cap
AR0-9978-12-C
Cert+ Vial Kit, 9 mm, Vial i3(Qsert) Amber w/ Patch + PE-Starburst cap
AR0-9979-12-C
Cert+ Vial Kit, 9 mm, PP, 300 L + PE-Starburst cap
Cert+ Vial Kit, 9 mm, PP, 700 L + PTFE/Silicone cap
Cert+ Vial Kit, 9 mm, PP, 700 L + PTFE/Silicone, preSlit cap
Price

Certified for cleanliness and enhanced quality
1000/pk
Price
AR0-9987-13-C
AR0-9988-13-C
AR0-9985-13-C
AR0-9986-13-C
12
AR0-9974-13-C
AR0-9978-13-C
AR0-9979-13-C
AR0-9995-13-C
AR0-9993-13-C
AR0-9994-13-C
*No write-on patch unless otherwise indicated
-B = Bonded-In Septa.
Cap is one piece, constructed of ultra-pure, medical-grade polyethylene.
Convenience Kits 9-425 Screw-Top Vials and Caps with Bonded-In Septa
Sample volume range from 0.2 to 1.5 mL
Description
Vial Kit, 9 mm, 2 mL Clear + PTFE/Silicone
Vial Kit, 9 mm, 2 mL Clear + PTFE/Silicone, preSlit
Vial Kit, 9 mm, 2 mL Clear w/ Patch + PTFE/Silicone
Vial Kit, 9 mm, 2 mL Clear w/ Patch + PTFE/Silicone, preSlit
Vial Kit, 9 mm, 2 mL Clear + PTFE/Silicone/PTFE
Vial Kit, 9 mm, 2 mL Clear + PTFE/Rubber
Vial Kit, 9 mm, 2 mL Clear w/ Patch + PTFE/Rubber, preSlit
Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Silicone
Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Silicone, preSlit
Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Silicone/PTFE
Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Rubber
Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Rubber, preSlit
Phenomenex
best seller
1000/pk
AR0-9901-13
AR0-9903-13
AR0-9921-13
AR0-9925-13
AR0-9905-13
AR0-9907-13
AR0-9914-13
AR0-9922-13
AR0-9926-13
AR0-9923-13
AR0-9912-13
AR0-9913-13
Price
Learn More. Visit Phenomenex.com/Verex

for additional product selection and detailed
information.
27
Verex Vials
12 x 32 mm, 9-425 (9 mm) Screw-Top Vials and Kits (contd)

Convenience Kits Specialty 9-425 Screw-Top Vials and Caps
with Bonded-In Septa
Kits for microsampling (center-draining and fused-insert vials)

CD and Qsert vials offer superior performance over vials with loose inserts
Polypropylene (PP) vial kits for bio- or ion-chromatography, or pH-sensitive samples

Description
Vial Kit, 9 mm, High Recovery (CD) Clear + PTFE/Silicone
Vial Kit, 9 mm, High Recovery (CD) Clear + PTFE/Silicone, preSlit
Vial Kit, 9 mm, Vial i3 (Qsert) Clear w/ Patch + PTFE/Silicone
Vial Kit, 9 mm, Vial i3 (Qsert) Clear w/ Patch + PTFE/Silicone, preSlit
Vial Kit, 9 mm, PP, 300 L + PTFE/Silicone
Vial Kit, 9 mm, PP, 300 L + PTFE/Silicone, preSlit
100/pk
AR0-9981-12
AR0-9982-12
AR0-9973-12
AR0-9974-12
Price
1000/pk
AR0-9981-13
AR0-9982-13
AR0-9973-13
AR0-9974-13
AR0-9991-13
AR0-9992-13
Price
*No write-on patch unless otherwise indicated
Convenience Kits Assembled 9-425 Screw-Top

Vials and Caps with Bonded-In Septa
Inserts Snap
for 9-425
Screw-Tops
to Vials,
SpecialtyVials
Cap and septa attached to vials

Ready to use
Save time and labor
Description
Vial Kit, 9 mm, 2 mL Clear w/ Patch + PTFE/Silicone, preSlit
Vial Kit, 9 mm, 2 mL Amber w/ Patch + PTFE/Silicone, preSlit
Vial Kit, 9 mm, 2 mL Clear, w/ Patch + PTFE/Rubber, Silanized
1000/pk
AR0-9925-13-A
AR0-9926-13-A
AR0-9915-13-A
Price
9-425 Screw-Top Vials, 2.0 mL

Used with most autosamplers, including Agilent, Thermo Scientific,

Waters and many others
Performs as well as crimp or snap vials
Offers improved cap convenience and accessibility (easy on, easy off)
Description
Vial, 9 mm Screw, 2 mL Clear, No Patch
Vial, 9 mm Screw, 2 mL Amber, No Patch
Vial, 9 mm Screw, 2 mL Clear, w/ Patch
Vial, 9 mm Screw, 2 mL Amber, w/ Patch
Vial, 9 mm Screw, 2 mL Clear, w/ Patch, Silanized
1000/pk
AR0-3900-13
AR0-3901-13
AR0-3910-13
AR0-3911-13
AR0-3960-13

Price
Specialty 9-425 Screw-Top Vials
Microsampling Qsert and v-Vial center-draining vial

Qsert fused-insert vials ensure proper seating of the cap
Extremely low residual volume
Description
Vial, 9 mm Screw, Vial i3 (Qsert) Clear, No Patch
Vial, 9 mm Screw, Vial i3 (Qsert) Amber, No Patch
Vial, 9 mm Screw, v-Vial Clear, No Patch
Vial, 9 mm Screw, v-Vial Amber, No Patch
28
100/pk
AR0-3920-12
AR0-3921-12
AR0-3940-12
AR0-3941-12
Price
1000/pk
AR0-3920-13
AR0-3921-13
AR0-3940-13
AR0-3941-13
Price
51-Expansion glass.
Phenomenex
Verex Vials
12 x 32 mm, 9-425 (9 mm) Screw-Top Vials and Kits (contd)

Certified PLUS (Cert+) Mass Spec Quality (MSQ) Caps
for 9-425 Screw-Top Vials
RIGHT CHOICE
HPLC and LC/MS tested and certified

For your most demanding analysis; high sensitive detectors
Locked-fit provides a positive physical septa lock into the cap
Description
Cert+ MSQ Cap (pre-assembled), 9 mm, w/ Locked-fit PTFE/Silicone septa, blue
Cert+ MSQ Cap (pre-assembled), 9 mm, w/ Locked-fit PTFE/Silicone preSlit septa, blue
1000/pk
AR0-8952-13-M
AR0-8972-13-M
MS Quality. Constructed of virgin

polymers, free of siloxanes and
trace contaminants, these caps
are the cleanest available from
Phenomenex.
Certified Plus
MS Quality
Price
Certified ultra-clean
High-grade, preconditioned raw materials
Finished product inspected and LC/MS tested
Description
Cert+ Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, blue
Cert+ Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, blue
Cert+ Cap (one-piece*), 9 mm PE* w/ Starburst preSlit, natural
2
1000/pk
AR0-8952-13-C
AR0-8972-13-C
AR0-89P6-13-C
Price
*Cap is one piece, constructed of ultra-pure, medical-grade polyethylene
Bonded-In Caps
For a more economical alternative,

Press-Fit Caps are available.
Contact your Phenomenex
technical consultant or distributor.
Bonded septa caps eliminate costly liner/septa fallout
Certified PLUS (Cert+) Caps


Vials
Inserts
for 9-425 Screw-Tops
InsertsVials
for 9-425 Screw-Tops Vials
Prevents rework and wasted productivity with perfect-fit septa

Saves instrument downtime
Description
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, black
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, blue
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, natural
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone septa, red
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, black
Cap (pre-assembled), 9 mm, w/ Bonded-in PTFE/Silicone preSlit septa, blue
1000/pk
AR0-8957-13-B
AR0-8952-13-B
AR0-8956-13-B
AR0-8951-13-B
AR0-8977-13-B
AR0-8972-13-B
Price
Inserts
For limited volume sampling in 2 mL sample vials Snap to Vials, Specialty Snap to Vials, Specialty Snap to Vials, Specialty
Virtually no adsorption or pH effects
Precisely centers and fits vial neck
Offers maximum opening to sampling needle
Description
Insert, 6 mm Diameter, Flat Bottom, 450 L
Insert, 6 mm Diameter, Flat Bottom, 450 L, Silanized
Insert, 6 mm Diameter, Conical Bottom, 350 L
Insert, 6 mm Diameter, Conical Bottom, w/ bottom spring, 300 L
Insert, 6 mm Diameter, Conical Bottom, w/ bottom spring, 300 L, Silanized
500/pk
AR0-4610-52
AR0-4615-52
Price
1000/pk
AR0-4620-13
AR0-4621-13
AR0-4623-13
Price
*Approximate useable volume indicated in L
Phenomenex
29
Verex Vials
12 x 32 mm, 8-425 (8 mm) Screw-Top Vials

Standard-neck vials for Varian and Shimadzu autosamplers

33-Expansion clear glass (USP Type 1 borosilicate, Class A), 51A amber glass
Description
1000/pk
AR0-3800-13
AR0-3801-13
AR0-3810-13
AR0-3811-13
Price
Caps / Closures
Prevent rework and wasted productivity with perfect
fit bonded-in
septa
Snap to Vials,
Specialty
Fewer trace contaminants for higher sensitivity work
Description
Cap (pre-assembled), 8-425, w/ Bonded-in PTFE/Silicone septa, black*
Cap (pre-assembled), 8-425, w/ Bonded-in PTFE/Silicone preSlit septa, black*
Cap (pre-assembled), 8-425, w/ PTFE/Silicone septa, black**
Cap (pre-assembled), 8-425, w/ PTFE/Silicone preSlit septa, black**
Cap (pre-assembled), 8-425, w/ PTFE/Silicone/PTFE septa, black**
Cap (pre-assembled), 8-425, w/ Open-top cap (w/o septa)
Cap (pre-assembled), 8-425, w/ Open-top cap (w/o septa, flanged lip)
*-B = Bonded-in Septa
**Press-Fit-Septa
1000/pk
AR0-8857-13-B
AR0-8877-13-B
AR0-8857-13
AR0-8877-13
AR0-8867-13
AR0-8897-13
AR0-88A7-13
Price
Inserts forVials
9-425 Screw-Tops
Vials
Inserts for
Septa
for 8-425 Screw Caps

Preconditioned and tested

For caps / closures without septa (AR0-8897-1312and
AR0-88A7-13)
Description
Septa, 8 mm Diameter, PTFE/Silicone 0.060 in
Septa, 8 mm Diameter, PTFE/Silicone/PTFE 0.060 in
Septa, 8 mm Diameter, PTFE 0.010 in
Inserts
1000/pk
AR0-6853-13
AR0-6863-13
AR0-6817-13
Price

For limited volume sampling

Transforms full capacity vials to micro sample vials
Description
Insert, 5 mm Diameter, Flat Bottom, 200 L, deactivated (silanized)
500/pk
AR0-4510-52
AR0-4514-52
Price
1000/pk
AR0-4510-13
AR0-4514-13
AR0-4520-13
AR0-4521-13
Price
Approximate useable volume indicated in L
30
x 32mm Specialty12
Vials
12 x 32mm Specialty12Vials
Phenomenex
Verex Vials
12 x 32 mm, 10-425 (10 mm) Screw-Top Vials and Kits

Convenience Kits 10-425 Screw-Top Vials and Caps with Bonded-In Septa
Available in assembled ready to use kits
Offered with silanization
Description
Vial Kit, 10 mm, 2 mL Clear, No Patch + PTFE/Silicone, preSlit
Vial Kit, 10 mm, 2 mL Clear, No Patch + PTFE/Silicone, Silanized
Vial Kit, 10 mm, 2 mL Clear, No Patch, PTFE/Silicone + preSlit, Silanized
Vial Kit, 10 mm, 2 mL Clear, w/ Patch + PTFE/Silicone*
Vial Kit, 10 mm, 2 mL Amber, w/ Patch + PTFE/Silicone*
Snap to Vials,
Specialty
100/pk
AR0-9005-12
AR0-9006-12
Price
1000/pk
AR0-9003-13
AR0-9021-13-A
AR0-9022-13-A
Price
*-A = Assembled
Easy-to-fill wide-neck vials

Description
Vial, 10 mm Screw, 2 mL Clear, No Patch, Silanized
1000/pk
AR0-3000-13
AR0-3010-13
AR0-3003-13
AR0-3001-13
AR0-3011-13
Price
13-425 Screw-Top Vials

Microsampling Qsert and center-draining vial

Extremely low residual volume
Description
Vial, 10 mm Screw, Vial i3 (Qsert) Clear, No Patch
Vial, 10 mm Screw, Vial i3 (Qsert) Amber, No Patch
Vial, 10 mm Screw, v-Vial, Clear, No Patch
100/pk
AR0-3020-12
AR0-3021-12
AR0-3040-12
Price
1000/pk
AR0-3020-13
AR0-3021-13
AR0-3040-13
Price
Inserts for
Inserts forVials
9-425 Screw-Tops
Vials
Caps / Closures
High quality preconditioned septa
Bonded-in or press-fit
Shell Vials
VOA / ASE
Prevent rework and wasted productivity with perfect fit bonded-in septa
Description
Cap (pre-assembled), 10-425, w/ Bonded-in PTFE/Silicone preSlit septa, black*
Cap (pre-assembled), 10-425, w/ PTFE/Silicone septa, black**
Cap (pre-assembled), 10-425, w/ PTFE/Silicone preSlit septa, black**
*-B = Bonded-in Septa
**Press-fit Septa
Inserts
1000/pk
AR0-8057-13-B
AR0-8077-13-B
AR0-8057-13
AR0-8077-13
Price

For limited volume sampling

Description
Insert, 6 mm Diameter, Flat Bottom, 450 L, Silanized
Insert, 6 mm Diameter, Conical Bottom w/ bottom spring, 300 L, Silanized
500/pk
AR0-4610-52
AR0-4615-52
Price
1000/pk
Price
Headspace Vials
AR0-4620-13
AR0-4621-13
AR0-4623-13
Approximate useable volume indicated in L
Phenomenex
31
Verex Vials
15 x 45 mm, 13-425 (13 mm) Screw-Top Vials and Kits

Convenience Kits 13-425 Screw-Top Vials and Caps
Original Waters 4 mL WISP autosampler vial
General purpose sample / standard storage vial
Description
Vial Kit, 4 mL Clear w/ Patch + screw caps 13-425, PTFE/Silicone*
Vial Kit, 4 mL Clear w/ Patch + screw caps 13-425, PTFE 0.01 in.*
Vial Kit, 4 mL, Amber w/Patch + screw caps 13-425, PTFE/Silicone*
Vial Kit, 4 mL Amber w/ Patch + screw caps 13-425, PTFE 0.01 in.*
*-A = Assembled.
1000/pk
AR0-9321-13-A
AR0-9391-13-A
AR0-9422-13-A
AR0-9392-13-A
Price
= Bonded-In Septa

Description
Vial, 4 mL Screw Clear, No Patch
Vial, 4 mL Screw Clear, w/ Patch
Vial, 4 mL Screw Amber, No Patch
1000/pk
AR0-3300-13
AR0-3310-13
AR0-3301-13
Price
100/pk
AR0-3370-12
Price

Center-draining bottom for low-volume samples
Description
Vial, 4 mL Screw High Recovery (CD) Clear, No Patch
1000/pk
AR0-3370-13
Price
VOA / ASE 13-425 Screw-Top Vials
Shell Vials
Caps / Closures for 13-425 Screw Top Vials

High quality, pre-conditioned septa
Available with bonded-in septa
Description
Cap (pre-assembled), 13-425, w/ PTFE/Silicone septa, black
*-B = Bonded-in Septa. = Press-fit
1000/pk
AR0-8357-13-B
AR0-8357-13
Price
Shell Vials
VOA / ASE
Shell Vials
Original 1 mL and 4 mL shell vials for Waters 96- and 48-position autosampler trays
Kits include high-quality borosilicate glass vials with convenient push-in polyethylene cap
Shell Vials
VOA / ASE
Convenience Kits Shell Vials, 1.0 and 4.0 mL

Description
Shell Vial Kit, 8 x 40 mm, 1 mL Flat Bottom, Clear + PE Cap
Shell Vial Kit, 15 x 45 mm, 4 mL Flat Bottom, Clear + PE Cap
Headspace Vials
1000/pk
AR0-3110-13
AR0-3170-13
Price
51-Expansion glass.
Convenience Kits Certified PLUS (Cert+) Shell Vials, 1.0 mL

Description
Cert+ Shell Vial Kit, 8 x 40 mm, 1 mL Flat Bottom, Clear + PE Cap
Cert+ Shell Vial Kit, 8 x 40 mm, 1 mL Flat Bottom, Amber + PE Cap
32
Headspace Vials
200/pk
AR0-3110-22-C
AR0-3111-22-C
Price
Headspace Vials
Phenomenex
Verex Vials
Shell Vials
Headspace Vials
Headspace Vials
Crimp-Top Headspace Vials
10 and 20 mL crimp neck, with round or flat bottom
Uniform glass thickness ensures even heating
Lot traceable
1000/pk
AR0-3220-13
AR0-3230-13
AR0-3260-13
AR0-3263-13
AR0-3290-13
AR0-3270-13
Price
Description
Headspace Vial, 23 x 46 mm, 10 mL Square Rim, Flat Bottom, Clear, No Patch
Headspace Vial, 23 x 46 mm, 10 mL Square Rim, Round Bottom, Clear, No Patch
Headspace Vial, 23 x 75 mm, 20 mL Beveled Rim, Flat Bottom, Clear, No Patch
Headspace Vial, 23 x 75 mm, 20 mL Beveled Rim, Flat Bottom., Clear, No Patch, Silanized
Headspace Vial, 23 x 75 mm, 20 mL Square Rim, Flat Bottom, Clear, No Patch
Headspace Vial, 23 x 75 mm, 20 mL Beveled Rim, Round Bottom, Clear, No Patch
Autosampler Compatibility
Flat Bottom: HP / Agilent, Carlo Erba, Shimadzu
Headspace Vials
Round Bottom: PerkinElmer, Tekmar, LEAP Technologies, Varian
Headspace Crimp Top Seals / Closures

Variety of styles for any application
Crimp and decrimp with ease
Magnetic and pressure-release caps available
Description
Seal, 20 mm Diameter, PTFE/Gray Butyl Rubber, magnetic cap
Seal, 20 mm Diameter, PTFE/Gray Butyl Rubber, Pressure Release, silver
Seal, 20 mm Diameter, PTFE/Butyl Rubber Pharmafix Molded Septum, silver
Seal, 20 mm Diameter, PTFE/Butyl Rubber Pressure Release, Pharmafix Molded Septum, silver
Seal, 20 mm Diameter, PTFE/Silicone magnetic cap
Seal, 20 mm Diameter, PTFE/Silicone silver
Seal, 20 mm Diameter, PTFE/Silicone Pressure Release, silver
1000/pk
AR0-52C5-13
AR0-52A0-13
AR0-52D0-13
AR0-52B0-13
AR0-5255-13
AR0-5250-13
AR0-5220-13
Price
Shell Vials
VOA / ASE
VOA / ASE Assembled Vial Kits and Storage Vial Kits

Shell Vials
VOA / ASE
Convenience and assembled kits include vials with matching seals / closures
Clear or amber, with open top or closed caps
PTFE (Teflon) faced 0.125 in. silicone septa or PTFE with foam urethane backing
Lot numbered for traceability
Description
Vial Kit, Storage, 20 mL screw, Clear w/ Caps 24-400 white PTFE/Foam Urethane liner, closed top
Vial Kit, Storage, 20 mL screw, Amber w/ Caps 24-400 white PTFE/Foam Urethane liner, closed top
Vial Kit, Storage, 40 mL screw, Clear w/ Caps 24-400 white PTFE/Foam Urethane, closed top
Vial Kit, Storage, 40 mL screw, Amber w/Caps 24-400 white PTFE/Foam Urethane, closed top
Vial Kit, VOA/ASE, 40 mL screw, Clear w/Caps 24-400 white PTFE/Silicone, open top
Vial Kit, VOA/ASE, 40 mL screw, Amber w/ Caps 24-400 white PTFE/Silicone, open top
Vial Kit, VOA/ASE, 20 mL screw, Clear w/ Caps 24-400 white PTFE/Silicone (assembled)*, open top
* EPA certified Class 100 vials, caps, and septa are assembled, without processing.
ASE = Dionex Accelerated Solvent Extractor for pestcide analysis.
Phenomenex
100/pk
AR0-9559-12
AR0-9551-12
AR0-9542-12
AR0-9543-12
AR0-9540-12
AR0-9541-12
AR0-9531-12-E
Price
1000/pk
AR0-9559-13
Headspace Vials
AR0-9551-13
Headspace
Vials
AR0-9542-13
AR0-9543-13
AR0-9540-13
AR0-9541-13
AR0-9531-13-E
Price
Learn More. Visit Phenomenex.com/Verex

for additional product selection
and detailed information
33
Verex Vials
Make the Switch to Verex!

Finding the Verex replacement to your current vials and caps is easy.
Use our online web tool to find the guaranteed Verex product match.
The compatibility of Verex Vials with various models of autosamplers is indicated in the table. An X mark indicates that the vials
from this category are compatible with the autosampler in most
configurations. Flanged indicates that a cap having an outer flange
is required for the vial to operate properly with the autosampler.
Magnetic indicates that a magnetic seal is required for use with
the autosampler.
www.Phenomenex.com/VialMatch
Autosampler Compatibility Chart
Manufacturer
Model
Products found on page:

Agilent Technologies
1050
1090A
1100/1200 Wellplate/ NanoFlow
6850ALS
7673A/7683
7694E
Beckman Coulter
501/507/System Gold
CTC
See Leap Technologies
Dionex
ASI-100/A550
AS-40
FAMOS
ESA
540
540-MT
Fisons
A-200S/AS 150/800/8000
Gerstel
MPS 2
MPS 3
Gilson
234, 235, 250
231 XL/232 XL/ 233 XL
215
Hitachi
AS-1000, AS-2000
AS-4000
Lachrom L-7200/7250
Lachrom Elite L-2200
Jasco
851/AS950/1550/1555
AS-2059/AS2059Plus
AS-2055/AS/2057
LEAP Technologies
CLPAL/GC PAL
Combi PAL
HTC PAL/HTS PAL
PerkinElmer
AutoSystem/ AS-2000/XL
Clarus 500/600
LC Plus
Shimadzu
AOC-8B/9, AOC-14/1400
AOC-5000
AOC-20
HSS-2B/4B
SIL-10A
SIL-HT/10ADVP, LC-2010
Spark-Holland
Symbiosis/ Reliance
Thermo Scientific
Surveyor LC
AS-3000/Trace GC/ Focus LC
TriPlus
Teledyne/Tekmar
7000/7050
HT3A
Varian
CP-8410
CombiPAL
8034/8035/8100/8200
9095/9100
Prostar 400/410/420
Prostar 430
Genesis
Waters
WISP 48 position tray
Alliance 2690
Model 2767
Model 2777
Acquity
CapLC
11 mm
Crimp
8-425 (8 mm)
Screw Thread
9-425 (9 mm)
Screw Thread
10-425 (10 mm)

Screw Thread
15 x 45 mm
4 mL
Headspace
25
x
x
x
x
x
30
27-29
x
x
x
31
32
33
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
Flanged
Flanged
Flanged
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
Magnetic
x
x
x
x
x
x
x
x
Magnetic
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
Magnetic
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
All trademarks belong to their respective owners
34
Phenomenex
Sample
Preparation
Sample Preparation
Sample Preparation. ........................................................................ 36-37

Polymeric Solid Phase Extraction

Strata-X............................................................................................... 38-46
Traditional Solid Phase Extraction

Strata.................................................................................................. 47-55
Biological Sample Cleanup

Strata Impact........................................................................................... 56
Sample Preparation Accessories

Vacuum Manifolds and Collection Plates........................................... 57-59
35 - 59
Sample Preparation
Sample Preparation Techniques
Strata-X and Strata SPE
No Method Development Required
Increase Column Lifetime
Removes Proteins
Removes Lipids
Removes Other Matrix Contaminants
Specific Selectivity for your Target Analytes
Concentrate Sample
De-Salt Sample
Perform Solvent Switch
X
X
X
X
X
X
X
X
Strata Impact Protein Precipitation Plates

X
X
X
Available Formats
Sample Preparation techniques / formats | Sample Preparation
Strata-X Polymeric SPE

Strata Traditional SPE
Strata Impact Protein Precipitation Plates
96-Well Plates
X
X
X
Giga Tubes
(12 mL - 150 mL)
X
X
On-line Extraction
Cartridge
X
X
Bulk Sorbent
X
X
Strata is a registered trademark of Phenomenex, Inc.

Strata-X, Giga, Sepra, and Impact are trademarks of Phenomenex, Inc.
Strata-X is patented by Phenomenex, Inc.
96-Well Plates
Syringe Tubes
On-line Extraction
Cartridges
Dont see the format you want? Contact

Phenomenex or your local Phenomenex
distributor for custom packed SPE phases
36
Syringe Tubes
X
X
Giga Tubes
Bulk Sorbent
For Sepra Bulk Sorbent Material Characteristics

and Ordering Information, see p. 352
Phenomenex
Sample Preparation
Sample Preparation Solutions
More than just a product, Strata-X and Strata sample preparation products are
supported by customer and technical resources that are dedicated to your satisfaction and success.
If Strata SPE products do not perform

as well or better than your current SPE
product of similar phase, mass and size,
return the product with comparative data
within 45 days for a full refund.
Sample Preparation Specialist

There is a Sample Preparation Specialist dedicated to your company.
Contact your Sample Preparation Specialist for:
Industry specific applications and technical resources
Product support
Method development assistance
Any question at all
The Solid Phase Extraction (SPE) method development tool allows you to quickly
take advantage of the sample cleanup benefits SPE offers.
Develop a method in under one minute
Customized SPE methods
Sample Preparation solutions
Online SPE Method Development Tool
Sample Preparation |
Contact your local

Phenomenex office to
speak with your Sample
Preparation Specialist
Sample pretreatment recommendations
Access the SPE Method

Development Tool via the
web at:
www.phenomenex.com/info/mdtool
Phenomenex
37
Strata -X Polymeric SPE
U.S. Patent No. 7,119,145
Strata-X
Strata-X Polymeric SPE | Sample Preparation
Benefits of Strata-X SPE vs. Traditional SPE

Strata-X
Polymeric SPE
Traditional SPE
Increase Detection Sensitivity by removing matrix

contaminants
Increase Column Lifetime by removing matrix

contaminants
Quality Guaranteed by more than 20 QA and QC

measures
Increase Reproducibility with robust methods
Save Time by processing multiple samples

simultaneously or automating method
Specific Selectivity for your target analytes
Decreased Solvent Consumption with the highest

loadability
Decreased Blow-down Time with smaller elution

volumes
Decreased Sample Variation with deconditioning

resistant sorbent
pH Stable from 1-14
Strata-X Method Development Kits

Sample Preparation Method Development Kits
Part No.
Description
Unit
Strata-X Method Development Kit
ea
KS0-7908 Strata-X 200 mg/3 mL tubes (10 ea)
Strata-X-C 200 mg/3 mL tubes (10 ea)
Strata-X-CW 200 mg/3 mL tubes (10 ea)
Strata-X-A 200 mg/3 mL tubes (10 ea)
Strata-X-AW 200 mg/3 mL tubes (10 ea)
Strata-X Method Development 96-Well Plate Kit for High-Throughput Screening
ea
KS0-8241 Strata-X 10 mg/well (3 rows)
Strata-X-C 10 mg/well (3 rows)
Strata-X-CW 10 mg/well (3 rows)
Strata-X-AW 10 mg/well (3 rows)
KS0-8209
Strata-X 30 mg/well (3 rows)

Strata-X-C 30 mg/well (3 rows)
Strata-X-CW 30 mg/well (3 rows)
Strata-X-AW 30 mg/well (3 rows)
Price
ea
For phase structures, turn to the phase

product pages, found on pp. 40 to 55
38
Phenomenex
Strata-X
Step 1. Select a Sorbent
Compound-Directed Phase Selection

Analyte pKa
Strata-X
Strata-XL
Neutral
10
11
12
13
14
Neutral Analyte or Analyte with log P > -0.5
Modified N-Vinylpyrrolidone functional group results in multiple mechanisms of retention for the widest
range of selectivity. Strong retention of neutral compounds allows for aggressive washes for increased
sensitivity and method reproducibility.
Acidic Analyte pKa 2
Dimethyl butyl quaternary amine functional group

provides strong retention of weakly acidic analytes
and allows for 100 % organic washes, increasing
Strata-X-A
Strata-XL-A
Strata-X-AW
Strata-XL-AW
Acidic Analyte pKa 5
Di-amino functional group is highly selective for charged

acidic compounds and allows for 100 % organic washes,
increasing sensitivity and method reproducibility.
Basic Analyte pKa 10.5
Sulfonic acid functional group is highly selective for weakly basic

compounds and allows for 100 % organic washes, increasing
Basic Analyte pKa 8
Carboxylic acid functional group allows for reversible binding

of strong bases and quaternary amines. High selectivity for all
basic compounds allows for 100 % organic washes, increasing
Strata-X-CW
Strata-XL-CW
Specialty Sorbents
Application
Strata-X-Drug N
Neutral Drugs of Abuse
Proprietary strong cation-exchange sorbent that eliminates the need to

condition/equilibrate the sorbent.
Basic Drugs of Abuse
Strata-X-Drug B
Phase Description
Sample Preparation
Strata-X-C
Strata-XL-C
Proprietary reversed phase sorbent that eliminates the need to

condition/equilibrate the sorbent.
Step 2. Select a Sorbent Mass

Loading Capacity Chart
Strata-X Phase
Strata-X, X-C, X-CW, X-A, X-AW
Strata-XL, XL-C, XL-CW, XL-A, XL-AW
Plasma
/Serum
Urine
Filtered Tissue
Homogenates
100 L
250 L
10 mg
250 L
1 mL
50 mg
500 L
2 mL
100 mg
N.R.
Water
(particulate-laden)
Mass
(mg in tube)
N.A.
N.A.
10 mg
N.R.
N.R.
30 mg
N.R.
60 mg
1 mL
4 mL
150 mg
50 mL
25 mL
100 mg
N.A.
8 mL
300 mg
100 mL
50 mL
200 mg
N.A.
20 mL
500 mg
500 mL
100 mL
500 mg
50 L
125 L
5 mg
N.A.
N.A.
10 mg
125 L
500 L
25 mg
N.R.
N.R.
30 mg
250 L
1 mL
50 mg
N.R.
N.R.
60 mg
500 L
2 mL
75 mg
25 mL
13 mL
100 mg
N.A.
4 mL
150 mg
50 mL
25 mL
200 mg
N.A.
10 mL
250 mg
250 mL
50 mL
500 mg
N.A. = Not Applicable (not commonly used)

N.R. = Not Recommended (may not provide expected results)
Phenomenex
Water
(particulate free)
See the following pages for specific phase details

and general extraction protocols.
39

within 45 days for a FULL REFUND.
Strata-X and Strata-XL

A reversed phase functionalized polymeric sorbent that gives strong retention of neutral,
acidic, or basic compounds under aggressive, high organic wash conditions.
Strata-X, 33 m, 85
High Concentration Samples

Small Target Analytes (< 10 kDa)
Large Target Analytes (> 10 kDa)
Large Volume Samples
Viscous Samples
4
4
4
O
..
N
..
N
Strata-X
Strata-XL
Sorbent
Format
Mass
Tube
30 mg
30 mg
60 mg
100 mg
100 mg
200 mg
200 mg
500 mg
500 mg
Giga Tube
500 mg
1g
1g
2g
5g
Teflon Tube
200 mg
Sorbent
Format
Mass
Tube
30 mg
60 mg
100 mg
200 mg
200 mg
500 mg
Giga Tube
2g
2g
5g
5g
10 g
96-Well Plate
30 mg
8B-S100-FBJ-T
3 mL (50/box)
200 mg
8B-S100-FDG-T
12 mL (20/box)
10 mg
30 mg
60 mg
8E-S100-AGB
8E-S100-TGB
8E-S100-UGB
2 Plates/Box
2 Plates/Box
2 Plates/Box
Part Number
Unit
8B-S100-TAK
8B-S100-TBJ
8B-S100-UBJ
8B-S100-EBJ
8B-S100-ECH
8B-S100-FBJ
8B-S100-FCH
8B-S100-HBJ
8B-S100-HCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
8B-S100-HDG
8B-S100-JDG
8B-S100-JEG
8B-S100-KEG
8B-S100-LFF
12 mL (20/box)
12 mL (20/box)
20 mL (20/box)
20 mL (20/box)
60 mL (16/box)
Price
Hydrophobic Interaction
Hydrogen Bonding Dipole-Dipole Interactions
..
N
3 Mechanisms of Retention
- Bonding
Strata-XL, 100 m, 300
4
4
Part Number
Unit
Price
8B-S043-TAK
8B-S043-UBJ
8B-S043-EBJ
8B-S043-FBJ
8B-S043-FCH
8B-S043-HCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S043-KDG
8B-S043-KEG
8B-S043-LEG
8B-S043-LFF
8B-S043-MFF
12 mL (20/box)
20 mL (20/box)
20 mL (20/box)
60 mL (16/box)
60 mL (16/box)
8E-S043-TGB
2 Plates/Box
* To control flow rate with Strata-XL, use a stopcock (AH0-6048)

when processing samples with a vacuum manifold.
96-Well Plate
On-line Extraction Cartridge

Description
Part Number
Strata-X on-line extraction
00M-S033-B0-CB
cartridge, 20 x 2.0 mm
Cartridge holder, 20 mm
CH0-5845
General Extraction Protocol

Method written for a 30 mg/1 mL tube
Condition
Unit/Box
ea
Price
ea
1mL Methanol followed by 1mL D.I. Water
Load
Recommended volume of sample (see page 39)
Wash
For Large Volume Cleanup/Flash Analysis, use Giga Tubes
For SPE Vacuum Manifolds and Accessories, see pp. 57-59
The General Extraction Protocol is a convenient starting
point for SPE method development. Further optimization
may be required to tailor the method to your specific needs.
40
1 mL 5-60% Methanol in D.I. Water. Allow sorbent to dry

for 30 seconds
Elute
2x 500 L 2% Formic acid in Methanol or Acetonitrile
Phenomenex

within 45 days for aO
FULL REFUND.
Strata-X-C and Strata-XL-C
=
O
)
4
4
=
=
+
Format
S O - H Sorbent Mass
Tube
O
30 mg
60 mg
100 mg
100 mg
200 mg
200 mg
500 mg
Giga Tube
2g
2g
5g
5g
10 g
96-Well Plate
30 mg
)
Price
Part Number
Unit
8B-S044-TAK
8B-S044-UBJ
8B-S044-EBJ
8B-S044-ECH
8B-S044-FBJ
8B-S044-FCH
8B-S044-HCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S044-KDG
8B-S044-KEG
8B-S044-LEG
8B-S044-LFF
8B-S044-MFF
12 mL (20/box)
20 mL (20/box)
20 mL (20/box)
60 mL (16/box)
60 mL (16/box)
8E-S044-TGB
2 Plates/Box
Price
=
=
=
=
)
)
S O- H
12 mL (20/box)
12 mL (20/box)
20 mL (20/box)
20 mL (20/box)
60 mL (16/box)
2 Plates/Box
2 Plates/Box
2 Plates/Box
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
Strata-XL-C
O
Unit

Description
Part Number
Strata-X-C on-line extraction
00M-S048-B0-CB
CH0-5845
S O- H
Strata-X-C
Ordering Information O
+
Format
Sorbent Mass
PartH
- Number
S
O
Tube
30 mg
8B-S029-TAK
30 mgO
8B-S029-TBJ
60 mg
8B-S029-UBJ
100 mg
8B-S029-EBJ
100 mg
8B-S029-ECH
O
200 mg
8B-S029-FBJ
+
200 mg
8B-S029-FCH
-H
S
O
500 mg
8B-S029-HBJ
500 mg
8B-S029-HCH
O
Giga Tube
500 mg
8B-S029-HDG
1g
8B-S029-JDG
1g
8B-S029-JEG
2g
8B-S029-KEG
5g
8B-S029-LFF
96-Well Plate
10 mg
8E-S029-AGB
30 mg
8E-S029-TGB
60 mg
8E-S029-UGB
S O- H
- Bonding
S O- H
4
+
S O - H4
)
Strong Cation-Exchange

Viscous Samples
Strata-XL-C, 100 m, 300
Strata-X-C, 33 m, 85
S O- H
A strong cation-exchange functionalized polymeric sorbent that allows for complete

retention of basic compounds with a pKa less than 10.5, making 100 % organic wash
conditions possible.

Unit/Box
ea
Price
ea
Condition
Load
Recommended volume of sample (pH 6-7) (see page 39)
Wash
1mL 0.1 N Hydrochloric acid in D.I. Water followed by 1mL
0.1 N Hydrochloric acid in Methanol
Elute
2x 500 L 5% Ammonium hydroxide in Methanol or
Acetonitrile
Phenomenex

41


A weak cation-exchange functionalized polymeric sorbent that allows for complete
retention of basic compounds with a pKa greater than 8, including quaternary amines,
making 100 % organic wash conditions possible.
Strata-X-CW and Strata-XL-CW

Viscous Samples
Strata-XL-CW, 100 m, 300
OH
OH
)
)
OH
Unit
8B-S035-TAK
8B-S035-TBJ
8B-S035-UBJ
8B-S035-ECH
O
8B-S035-FBJ
8B-S035-FCH
OH
8B-S035-HBJ
8B-S035-HCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
8B-S035-HDG
8B-S035-JDG
8B-S035-JEG
8B-S035-KEG
8B-S035-LFF
12 mL (20/box)
12 mL (20/box)
20 mL (20/box)
20 mL (20/box)
60 mL (16/box)
8E-S035-AGB
8E-S035-TGB
8E-S035-UGB
2 Plates/Box
2 Plates/Box
2 Plates/Box
OH
Strata-XL-CW
O
O
Part Number
OH
OH

Description
Part Number
Strata-X-CW on-line extraction
00M-S036-B0-CB
CH0-5845
- Bonding
)
)
)
)
4
4
4
Sorbent
Format
Mass
Tube
30 mg
30 mg
60 mg
100 mg
200 mg
200 mg
500 mg
500 mg
Giga Tube
500 mg
1g
1g
2g
5g
96-Well Plate
10 mg
30 mg
60 mg
OH
4
4
Weak Cation-Exchange
Strata-X-CW
O
Strata-X-CW, 33 m, 85
Price
Sorbent
Format OH
Mass
Tube
30 mg
60 mg
100 mg
100 mg
200 mg
200 mg
500 mg
Giga Tube
2g
2g
5g
5g
10 g
96-Well Plate
30 mg
Part Number
Unit
Price
8B-S052-TAK
8B-S052-UBJ
8B-S052-EBJ
8B-S052-ECH
8B-S052-FBJ
8B-S052-FCH
8B-S052-HCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S052-KDG
8B-S052-KEG
8B-S052-LEG
8B-S052-LFF
8B-S052-MFF
12 mL (20/box)
20 mL (20/box)
20 mL (20/box)
60 mL (16/box)
60 mL (16/box)
8E-S052-TGB
2 Plates/Box

Unit/Box
ea
Price
ea
Condition
Load
Wash
42
1mL D.I. Water followed by 1mL Methanol
Elute
Phenomenex

CH3
N+
A strong anion-exchange functionalized polymeric sorbent that allows for complete

retention of weakly acidic compounds with pKa greater than 2, making 100 % organic
wash conditions possible.
Strata-X-A and Strata-XL-A
Strata-X-A, 33 m, 85

Viscous Samples
Strata-XL-A, 100 m, 300
N+
CH3
Strata-XL-A
CH
Unit
CH3
CH3
Strata-X-A
Price
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
12 mL (20/box)
12 mL (20/box)
20 mL (20/box)
20 mL (20/box)
60 mL (16/box)
2 Plates/Box
2 Plates/Box
2 Plates/Box
3
Ordering
Information
+
Sorbent
N
Format
Mass
CHTube
3
30 mg
60 mg
100 mg
100 mg
200 mg
200 mg
500 mg
Giga Tube
2g
2g
5g
5g
10 g
96-Well Plate
30 mg
Part Number
Unit
8B-S053-TAK
8B-S053-UBJ
8B-S053-EBJ
8B-S053-ECH
8B-S053-FBJ
8B-S053-FCH
8B-S053-HCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S053-KDG
8B-S053-KEG
8B-S053-LEG
8B-S053-LFF
8B-S053-MFF
12 mL (20/box)
20 mL (20/box)
20 mL (20/box)
60 mL (16/box)
60 mL (16/box)
8E-S053-TGB
2 Plates/Box
Price
CH3
N+
CH3
CH3
CH3
N+
CH3
N+
CH3
Sorbent +
Format
Mass N Part Number
Tube
CH3
30 mg
8B-S123-TAK
30 mg
8B-S123-TBJ
60 mg
8B-S123-UBJ
100 mg
8B-S123-EBJ
CH
3
100 mg
8B-S123-ECH
200 mg N+ 8B-S123-FBJ
200 mg
8B-S123-FCH
CH
500
mg3
8B-S123-HBJ
500 mg
8B-S123-HCH
Giga Tube
500 mg
8B-S123-HDG
1g
8B-S123-JDG
1g
8B-S123-JEG
2g
8B-S123-KEG
5g
8B-S123-LFF
96-Well Plate
10 mg
8E-S123-AGB
30 mg
8E-S123-TGB
60 mg
8E-S123-UGB
CH3
N+
- Bonding
4
4
4
CH3
Strong Anion-Exchange
CH3
4
4

Condition
Load
Wash
Phenomenex
1mL 25 mM Ammonium acetate (pH 6-7) followed by 1mL Methanol
Elute
43

Strata-X-AW and Strata-XL-AW

Viscous Samples
NH2
NH
NH2
NH2
NH2
NH
NH2
NH
NH
Strata-XL-AW
NH2
)
)
NH
NH2
2
NH
Sorbent
Format
Mass
Part Number
Tube
30 mg
8B-S038-TAK
30 mg
8B-S038-TBJ
60 mg
8B-S038-UBJ
100 mg
8B-S038-EBJ
NH2
100 mg NH8B-S038-ECH
200 mg
8B-S038-FBJ
200 mg
8B-S038-FCH
500 mg
8B-S038-HBJ
500 mg
8B-S038-HCH
Giga Tube
500 mg
8B-S038-HDG
1g
8B-S038-JDG
1g
8B-S038-JEG
2g
8B-S038-KEG
5g
8B-S038-LFF
96-Well Plate
10 mg
8E-S038-AGB
30 mg
8E-S038-TGB
60 mg
8E-S038-UGB
4
4
4
- Bonding
)
)
NH
Strata-X-AW
NH
4
4
Weak Anion-Exchange
Strata-XL-AW, 100 m, 300
Strata-X-AW, 33 m, 85
A weak anion-exchange functionalized polymeric sorbent that allows for complete

retention of acidic compounds with pKa less than 5, making 100 % organic wash
conditions possible.
Unit
Price
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
12 mL (20/box)
12 mL (20/box)
20 mL (20/box)
20 mL (20/box)
60 mL (16/box)
NH Information
Ordering
Sorbent
Format
Mass
Tube
30 mg
60 mg
100 mg
100 mg
200 mg
200 mg
500 mg
Giga Tube
2g
2g
5g
5g
10 g
96-Well Plate
30 mg
Part Number
Unit
Price
8B-S051-TAK
8B-S051-UBJ
8B-S051-EBJ
8B-S051-ECH
8B-S051-FBJ
8B-S051-FCH
8B-S051-HCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S051-KDG
8B-S051-KEG
8B-S051-LEG
8B-S051-LFF
8B-S051-MFF
12 mL (20/box)
20 mL (20/box)
20 mL (20/box)
60 mL (16/box)
60 mL (16/box)
8E-S051-TGB
2 Plates/Box
2 Plates/Box
2 Plates/Box
2 Plates/Box

Condition
Load
44
Wash
1mL 25 mM Ammonium acetate (pH 6-7) followed by 1mL Methanol
Elute
2x 500 L 5% Ammonium hydroxide in Methanol or Acetonitrile
Phenomenex
Strata-X-Drug B
A proprietary strong cation-exchange sorbent that is designed and quality controlled for basic
drugs of abuse analysis. This sorbent does not require a conditioning/equilibrating step.

Strata-X-Drug B Certificate of Analysis
We test recovery by extracting

codeine and morphine from
urine to ensure that each batch
provides you with the best
results possible!
Strong cation-exchange sorbents can

sometimes promote conversion of
norcodeine and normorphine to parent
compounds. Strata-X-Drug B is guaranteed
not to promote this interconversion.
Sorbent
Format
Mass
Tube
10 mg
10 mg
30 mg
30 mg
30 mg
60 mg
60 mg
60 mg
Giga Tube
100 mg
Part Number
Unit
8B-S128-AAK
8L-S128-AAK
8B-S128-TAK
8L-S128-TAK
8B-S128-TBJ
8B-S128-UBJ
8B-S128-UCH
8B-S128-UCL
1 mL (100/box)
1 mL (100/box)
1 mL (100/box)
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
6 mL (200/box)
8B-S128-EDG
12 mL (20/box)
8E-S128-AGB
8E-S128-TGB
8E-S128-UGB
2 Plates/box
2 Plates/box
2 Plates/box
Price
Opiates, 6-MAM, PCP,

Amphetamines,
Methadone, Healthcare
Opiates, and
Propoxyphene*
Marijuana Metabolites
Cocaine Metabolites
Condition
Tab-less
tube
Not Required
Load Pre-treated urine sample Pre-treated urine sample Pre-treated urine sample
Wash 1 2 mL of 100 mM
Sodium acetate
buffer (pH 5.0)
2 mL of 100 mM
Sodium acetate
buffer (pH 5.0)
2 mL of 0.1 N
Hydrochloric acid
Wash 2 2 mL Methanol
2 mL of Acetonitrile:100
mM Sodium acetate
buffer (pH 5.0) (30:70)
2 mL Methanol
15 minutes under
full vacuum
10 minutes under
full vacuum
2 mL of Ethyl acetate:
Isopropanol
(85:15)
Isopropanol: Ammonium
hydroxide (70:20:10)
96-Well Plate
10 mg
30 mg
60 mg
Strata-X-Drug B Extraction Protocols
Dry 10 minutes under

full vacuum
Elute 2 mL of Ethyl acetate:
Isopropanol: Ammonium
hydroxide (70:20:10)
* Opiates, 6-MAM, PCP, Amphetamines, Methadone, Healthcare Opiates, and Propoxyphene can be extracted simultaneously or separately using the same SPE methodology.
Methods are written for 60 mg/6 mL Strata-X-Drug B; however they can be scaled to
accommodate smaller or larger sample sizes and sorbent masses.
Phenomenex
45

Strata-X-Drug N
A proprietary reversed phase sorbent that is designed and quality controlled for neutral
drugs of abuse analysis. This sorbent does not require a conditioning/equilibrating step.
Strata-X-Drug N Certificate of Analysis
We test recovery by extracting lorazepam and

temazepam from urine to ensure that each batch
provides you with the best results possible!
Sorbent
Format
Mass
Tube
10 mg
10 mg
30 mg
30 mg
30 mg
60 mg
60 mg
60 mg
100 mg
Giga Tube
100 mg
Strata-X-Drug N Extraction Protocols

Part Number
Unit
8B-S129-AAK
8L-S129-AAK
8B-S129-TAK
8L-S129-TAK
8B-S129-TBJ
8B-S129-UBJ
8B-S129-UCH
8B-S129-UCL
8B-S129-ECH
1 mL (100/box)
1 mL (100/box)
1 mL (100/box)
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
6 mL (200/box)
6 mL (30/box)
8B-S129-EDG
12 mL (20/box)
8E-S129-AGB
8E-S129-TGB
8E-S129-UGB
2 Plates/box
2 Plates/box
2 Plates/box
96-Well Plate
10 mg
30 mg
60 mg
Tab-less
46
Price
Barbiturates
Benzodiazepines
Condition
Load
Not Required
Pre-treated urine sample
Pre-treated urine sample
Wash 1
2 mL of 0.1 N Hydrochloric
acid (HCI)
2 mL of Acetonitrile:Water (20:80)
Wash 2
2x 2 mL of Methanol:
0.1 N HCI (30:70)
Dry
Elute
10 minutes under full vacuum
10 minutes under full vacuum
Isopropanol (85:15)
Isopropanol (85:15)
Methods are written for 100 mg/6 mL Strata-X-Drug N; however they can be scaled to
accomodate smaller or larger sample sizes and sorbent masses.
tube
Phenomenex

Strata Traditional Solid Phase

Extraction (SPE) Sorbents
Material Characteristics
Phase
Particle Size
(m)
Pore Size
()
Surface Area
(m2/g)
Carbon Load
(%)
Bonding
End Capping
Ionic Capacity
(meq/g)
55
55
55
55
55
70
70
140
70
70
500
500
300
500
500
18.0
17.0
15.0
10.5
10.5
trifunctional
trifunctional
trifunctional
trifunctional
trifunctional
Yes
No
Yes
Yes
Yes
55
55
55
70
70
65
500
500
550
10.0
5.0
0.0
trifunctional
trifunctional
No
No
1.3
55
55
55
70
70
70
500
500
500
9.0
8.0
6.5
trifunctional
trifunctional
trifunctional
No
No
No
0.9
0.8
0.9
200
55
55
55
70
70
70
70
500
500
500
500
proprietary
proprietary
proprietary
7.0
trifunctional
trifunctional
trifunctional
170
200
80
70
300
proprietary
0.0
0.0
120
100
proprietary
proprietary
proprietary
120
260
proprietary
proprietary
proprietary
150
500
proprietary
proprietary
proprietary
proprietary
proprietary
Reversed Phase
C18-E
C18-U
C18-T
C8
Phenyl
Normal Phase
CN
NH2
Silica (Si-1)
Ion-Exchange
SCX
WCX
SAX
Mixed-Mode
Screen-C GF
Screen-C
Screen-A
ABW
Specialty
Strata traditional spe
FL-PR (Florisil)
EPH (Extractable Petroleum
Hydrocarbon)
AL-N (Alumina-Neutral)
SDB-L
Eco-Screen
Melamine
PAH
Over 20 QA and QC Tests Ran on Strata SPE Products

Surface Area
% Fe2O3
Flow Rate
Pore Size
Purity
Tube Purity
Pore Volume
Carbon Loading
Frit Purity
Surface pH
Hydrogen Loading
Dye Tests
Particle Diameter
Surface Coverage
Chromatogram
Particle Size Distribution
Residue
11 Probes Used
% Water
Turbidity
% SiO2
Sorbent Weight
Guaranteed Performance Contaminate-free Extracts

Phenomenex
Strata C18-E
Waters
Sep-Pak C18
Biotage IST
Isolute C-18(EC)
Varian
Bond Elut C18
Phenomenex
47

Determine the Correct Sorbent Mass

Silica-Based Sorbents
(Strata C18-E, C8, SCX, SAX, WCX, NH2, etc.)
Sample Matrix
Sorbent Mass
Blood, serum, plasma

Urine
Filtered tissue homogenates
50 mg sorbent per 250 L

50 mg sorbent per 500 L
100 mg sorbent per
100 mg tissue
Environmental Samples
Sorbent Mass
Water (particulate-free) drinking
500 mg/100 mL - 500 mL

sample
1 g/100 mL - 500 mL
sample
1 g/100 g of soil extract
Water (particulate-laden) rivers, runoff, etc.

Soil Extracts
Determine the Correct Sorbent Wash and Elution Volumes

10 mg
50 mg
100 mg
150 mg
200 mg
500 mg
1g
2g
5g
10 g
Practical Minimum Wash and Elution

Volume 4 bed volumes
60 L 300 L
600 L
900 L
1.2 mL
3 mL
6 mL
12 mL
30 mL
60 mL
Recommended Wash and

Elution Volume 8 bed volumes
120 L 600 L
1.2 mL
1.8 mL
2.4 mL
6 mL
12 mL 24 mL
60 mL
120 mL
Strata traditional SPE
Sample Preparation
Silica-Based Sorbent Mass

Strata Reversed Phase Method
Strata Ion-Exchange Method
Strata Normal Phase Method
for C18, C8, Phenyl, CN, SDB-L Sorbents
for SCX, WCX, SAX, NH2 (WAX) Sorbents
for Silica, Florisil, NH2, CN Sorbents
ANALYTE PROPERTIES
Moderate to high polarity compounds
(neutralized/uncharged)
SAMPLE/MATRIX
Aqueous; low ionic strength buffers (<30 mM), pH adjusted
CONDITIONING STEP
1. Conditioning polar organic solvents
2. Equilibration low ionic strength buffers, pH adjusted
SAMPLE/MATRIX
Non-polar organic solvents or moderately polar
organic solvents
CONDITIONING STEP
1. Solvation polar organic solvent
2. Equilibration sample/matrix solvent (non-polar)
WASH STEP
Non-polar organic solvents with a low concentration
(1 to 5 %) of moderate to low polarity organic solvents
ELUTION STEP
Non-polar organic solvents containing higher
concentrations (5 to 50 %) of moderate to high
polarity organic solvents
1
ANALYTE PROPERTIES
Low to moderate polarity (or non-polar), hydrophobic,
neutralized/uncharged
ANALYTE PROPERTIES
Ionized/charged compounds
SAMPLE/MATRIX
Aqueous, diluted with buffer
CONDITIONING STEP
1. Solvation polar organic solvents
2. Equilibration aqueous, buffers
WASH STEP
Aqueous buffers with 5 to 50 % polar organic solvent
ELUTION STEP
Polar or non-polar organic solvent(s) with or without
water, buffer and/or strong acid or base
WASH STEP
Aqueous buffers of low salt concentrations with
or without organic solvent
ELUTION STEP
Neutralize the charge on the weak anion or cation
Increase the ionic strength and counter ion concentration
Add a strong counter ion displacer
Elute using a polar organic
Suggested Elution Solvents
Polarity
THF
Increasing
Polarity
For complete ionization sample should be adjusted 2

pH units above or below the pKa of analyte. pH can be
used to effectively neutralize sorbent or analyte. This
can be accomplished by combining 2 % strong acid
or base with a water miscible organic solvent such as
methanol or acetonitrile. [As an alternative method,
high ionic strength buffer can be used to displace the
analyte, which may not be ideal for analysis by sensitive
detection instruments such as a mass spec].
Hexane
Acetone
Ethyl Acetate
Acetonitrile**
Isopropanol
Methanol
Polar
** when using aromatic sorbents such as Phenyl or

SDB-L, acetonitrile is a stronger elution solvent than
methanol **
Methylene Chloride
THF
Polarity
Most Non-polar
Increasing
Polarity
Acetone
Acetonitrile
Polar
Isopropanol
The above is a convenient starting point for SPE method

development. Further optimization may be required to tailor
the method to your specific needs.
48
Phenomenex

Reversed Phase Sorbents

C18-E
C18-U
End-capped C18 sorbent that offers strong hydrophobic

retention with negligible secondary polar interactions from
active silanol groups.
C18 sorbent with no end-capping, giving the phase moderate

hydrophobic selectivity with slight polar selectivity due to the
active silanol groups.
O
O
O
O
Si
O
O
O
Si
O
Si
Si
Unit
8B-S001-DAK
8B-S001-EAK
8B-S001-EBJ
8B-S001-FBJ
8B-S001-FCH
8B-S001-HBJ
8B-S001-HCH
8B-S001-JEG
1 mL (100/box)
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
20 mL (20/box)
8B-S001-HDG
8B-S001-KDG
8B-S001-LEG
8B-S001-MFF
8B-S001-VFF
8B-S001-YSN
8B-S001-ZSN
12 mL (20/box)
12 mL (20/box)
20 mL (20/box)
60 mL (16/box)
60 mL (16/box)
150 mL (8/box)
150 mL (8/box)
8E-S001-CGB
8E-S001-DGB
8E-S001-EGB
2 Plates/Box
2 Plates/Box
2 Plates/Box
Price
Sorbent
Format
Mass
Tube
100 mg
200 mg
500 mg
500 mg
1g
96-Well Plate
50 mg
100 mg
Unit
8B-S002-EAK
8B-S002-FBJ
8B-S002-HBJ
8B-S002-HCH
8B-S002-JCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8E-S002-DGB
8E-S002-EGB
2 Plates/Box
2 Plates/Box
Price
C18-T
A wide-pore C18 sorbent that offers strong hydrophobic
selectivity and accommodates molecules up to 75 kD
in size.
O
O
Unit/Box
ea
Part Number
Part Number

Description
Part Number
Strata C18-E on-line extraction
00M-S039-B0-CB
CH0-5845
Price
ea

Phenomenex
OH
Sorbent
Format
Mass
Tube
50 mg
100 mg
100 mg
200 mg
200 mg
500 mg
500 mg
1g
Giga Tube
500 mg
2g
5g
10 g
20 g
50 g
70 g
96-Well Plate
25 mg
50 mg
100 mg
Si
Si
O
O
O
Si
Si
Sorbent
Format
Mass
Tube
100 mg
200 mg
500 mg
500 mg
1g
96-Well Plate
25 mg
50 mg
Part Number
Unit
8B-S004-EAK
8B-S004-FBJ
8B-S004-HBJ
8B-S004-HCH
8B-S004-JCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8E-S004-CGB
8E-S004-DGB
2 Plates/Box
2 Plates/Box
Price
49

Reversed Phase Sorbents

C8
Phenyl
An end-capped C8 sorbent that offers moderate hydrophobic

retention with negligible secondary polar interactions from active
silanol groups.
A short alkyl chain with a phenyl group provides moderate

hydrophobic selectivity and aromatic selectivity through -
interactions.
O
O
Si
O
O
Sample Preparation
Si
Si
Sorbent
Format
Mass
Tube
100 mg
200 mg
500 mg
500 mg
1g
Giga Tube
2g
5g
10 g
96-Well Plate
25 mg
50 mg
100 mg
Si
Part Number
Unit
8B-S005-EAK
8B-S005-FBJ
8B-S005-HBJ
8B-S005-HCH
8B-S005-JCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S005-KDG
8B-S005-LEG
8B-S005-MFF
12 mL (20/box)
20 mL (20/box)
60 mL (16/box)
8E-S005-CGB
8E-S005-DGB
8E-S005-EGB
2 Plates/Box
2 Plates/Box
2 Plates/Box

Description
Part Number
Strata C8 on-line extraction
00M-S101-B0-CB
CH0-5845
Unit/Box
ea
Price
Si
Si
Sorbent
Format
Mass
Tube
100 mg
200 mg
500 mg
500 mg
1g
96-Well Plate
25 mg
50 mg
100 mg
Part Number
Unit
Price
8B-S006-EAK
8B-S006-FBJ
8B-S006-HBJ
8B-S006-HCH
8B-S006-JCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8E-S006-CGB
8E-S006-DGB
8E-S006-EGB
2 Plates/Box
2 Plates/Box
2 Plates/Box
Price
ea

Don't see the size or format you want? Contact Phenomenex

or your local distributor for other dimensions, Giga tubes, and
bulk sorbent pricing, and part numbers.
50
Phenomenex

Normal Phase Sorbents

Silica
(Si-1)
NH2/WAX
Unbonded silica particle that offers strong polar selectivity.
This amino phase offers strong polar selectivity and hydrogen

bonding under normal phase conditions or can be used as a weak
anion-exchange sorbent.
O
O
O
Si
OH
O
O
Si
O
O
OH
NH2
Part Number
Unit
8B-S012-EAK
8B-S012-FBJ
8B-S012-HBJ
8B-S012-HCH
8B-S012-JCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S012-HDG
8B-S012-JDG
8B-S012-KDG
8B-S012-LEG
8B-S012-MFF
8B-S012-VFF
8B-S012-YSN
8B-S012-ZSN
12 mL (20/box)
12 mL (20/box)
12 mL (20/box)
20 mL (20/box)
60 mL (16/box)
60 mL (16/box)
150 mL (8/box)
150 mL (8/box)
8E-S012-DGB
8E-S012-EGB
2 Plates/Box
2 Plates/Box
Price
Cyano
(CN)
A polar phase with slight hydrophobic selectivity in reversed phase
mode and moderate polar selectivity in normal phase mode.
O
Si
C
N
O
Si
Phenomenex
OH
Sorbent
Format
Mass
Tube
100 mg
200 mg
500 mg
500 mg
1g
Giga Tube
500 mg
2g
5g
10 g
20 g
96-Well Plate
25 mg
50 mg
100 mg
Sorbent
Format
Mass
Tube
100 mg
200 mg
500 mg
500 mg
1g
Giga Tube
2g
Part Number
Unit
8B-S009-EAK
8B-S009-FBJ
8B-S009-HBJ
8B-S009-HCH
8B-S009-JCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S009-HDG
8B-S009-KDG
8B-S009-LEG
8B-S009-MFF
8B-S009-VFF
12 mL (20/box)
12 mL (20/box)
20 mL (20/box)
60 mL (16/box)
60 mL (16/box)
8E-S009-CGB
8E-S009-DGB
8E-S009-EGB
2 Plates/Box
2 Plates/Box
2 Plates/Box
Part Number
Unit
8B-S007-EAK
8B-S007-FBJ
8B-S007-HBJ
8B-S007-HCH
8B-S007-JCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S007-KDG
12 mL (20/box)
8E-S007-DGB
8E-S007-EGB
2 Plates/Box
2 Plates/Box
Price
Sorbent
Format
Mass
Tube
100 mg
200 mg
500 mg
500 mg
1g
Giga Tube
500 mg
1g
2g
5g
10 g
20 g
50 g
70 g
96-Well Plate
50 mg
100 mg
Si
NH2
Si
Price
96-Well Plate
50 mg
100 mg
51

Ion-Exchange Sorbents
SAX
(strong anion-exchange)
WCX
(weak cation-exchange)
The quaternary amine phase remains positively charged under

all conditions, giving a strong anion-exchange mechanism of
retention.
A carboxylic acid group is bonded to the surface of the silica

particle, giving a weak cation-exchange selectivity.
O
O
O
O
Cl-
Si
Si
OH
Sample Preparation
|
Sorbent
Format
Mass
Tube
100 mg
100 mg
200 mg
500 mg
500 mg
1g
Giga Tube
500 mg
2g
5g
10 g
20 g
96-Well Plate
25 mg
50 mg
100 mg
Part Number
Unit
Price
8B-S008-EAK
8B-S008-EBJ
8B-S008-FBJ
8B-S008-HBJ
8B-S008-HCH
8B-S008-JCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S008-HDG
8B-S008-KDG
8B-S008-LEG
8B-S008-MFF
8B-S008-VFF
12 mL (20/box)
12 mL (20/box)
20 mL (20/box)
60 mL (16/box)
60 mL (16/box)
8E-S008-CGB
8E-S008-DGB
8E-S008-EGB
2 Plates/Box
2 Plates/Box
2 Plates/Box
SCX
(strong cation-exchange)
A benzene sulfonic acid group is bonded to the surface of the silica
particle, giving strong cation-exchange selectivity.
O
Si
O
O
O
52
Si
OH
OH
O
O
Si
Si
OH
O H
Sorbent
Format
Mass
Tube
100 mg
200 mg
500 mg
500 mg
1g
Giga Tube
2g
5g
10 g
96-Well Plate
25 mg
50 mg
100 mg
Sorbent
Format
Mass
Tube
100 mg
100 mg
200 mg
500 mg
500 mg
1g
Giga Tube
2g
5g
10 g
20 g
96-Well Plate
25 mg
50 mg
100 mg
Part Number
Unit
Price
8B-S027-EAK
8B-S027-FBJ
8B-S027-HBJ
8B-S027-HCH
8B-S027-JCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S027-KDG
8B-S027-LEG
8B-S027-MFF
12 mL (20/box)
20 mL (20/box)
60 mL (16/box)
8E-S027-CGB
8E-S027-DGB
8E-S027-EGB
2 Plates/Box
2 Plates/Box
2 Plates/Box
Part Number
Unit
Price
8B-S010-EAK
8B-S010-EBJ
8B-S010-FBJ
8B-S010-HBJ
8B-S010-HCH
8B-S010-JCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S010-KDG
8B-S010-LEG
8B-S010-MFF
8B-S010-VFF
12 mL (20/box)
20 mL (20/box)
60 mL (16/box)
60 mL (16/box)
8E-S010-CGB
8E-S010-DGB
8E-S010-EGB
2 Plates/Box
2 Plates/Box
2 Plates/Box
Phenomenex

Mixed-Mode Sorbents
Screen-C
ABW
Incorporates the hydrophobic selectivity of a C8 phase and strong

cation-exchange for the extraction of basic drugs from biological
matrixes.
Offers a strong cation-exchange group and a weak anion-exchange

group for the extraction or fractionation of complex mixtures.
O
O
O
Si
O H
O
O
8B-S016-EAK
8B-S016-EBJ
8B-S016-SBJ
8B-S016-SCH
8B-S016-FBJ
8B-S016-FCH
8B-S016-RBJ
8B-S016-RCH
8B-S016-HCH
1 mL (100/box)
3 mL (50/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S016-KDG
12 mL (20/box)
8E-S016-DGB
8E-S016-EGB
2 Plates/Box
2 Plates/Box
Price
Offers the selectivity of Screen-C in a gravity flow particle size for

viscous samples.
Part Number
Unit
8B-S026-FBJ
8B-S026-FCH
8B-S026-HBJ
8B-S026-HCH
8B-S026-JCH
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S026-HDG
8B-S026-KEG
8B-S026-LFF
12 mL (20/box)
20 mL (20/box)
60 mL (16/box)
Unit
8B-S030-FBJ
8B-S030-JCH
3 mL (50/box)
6 mL (30/box)
8B-S030-KDG
8B-S030-LEG
8B-S030-MFF
8B-S030-VFF
12 mL (20/box)
20 mL (20/box)
60 mL (16/box)
60 mL (16/box)
Price
Incorporates the hydrophobic selectivity of a C8 phase and strong

anion-exchange for the extraction of acidic drugs from biological
matrixes.
Screen-C GF
Sorbent
Format
Mass
Tube
200 mg
200 mg
500 mg
500 mg
1g
Giga Tube
500 mg
2g
5g
Part Number
Screen-A
96-Well Plate
50 mg
100 mg
Sorbent
Format
Mass
Tube
200 mg
1g
Giga Tube
2g
5g
10 g
20 g
Price
Unit
NH2
Part Number
Si
Sorbent
Format
Mass
Tube
100 mg
100 mg
150 mg
150 mg
200 mg
200 mg
300 mg
300 mg
500 mg
Giga Tube
2g
Phenomenex
Si
O
Si
O H
O
O
Si
Cl
O
O
Si
O
Sorbent
Format
Mass
Tube
100 mg
200 mg
200 mg
500 mg
96-Well Plate
25 mg
Part Number
Unit
8B-S019-EAK
8B-S019-FBJ
8B-S019-FCH
8B-S019-HCH
1 mL (100/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8E-S019-CGB
2 Plates/Box
Price
53

Alumina-N
(AL-N)
Florisil
(FL-PR, pesticide residue grade)
A polar phase that allows for the extraction of polar compounds

from food and environmental samples.
A modified silica sorbent that contains a magnesium ion, allowing for

the retention of polar and halogenated compounds, like pesticides,
from environmental samples.
Sorbent
Format
Mass
Tube
500 mg
1g
Giga Tube
2g
Sorbent
Format
Mass
Tube
500 mg
500 mg
1g
Giga Tube
1g
2g
5g
10 g
Part Number
Unit
8B-S313-HBJ
8B-S313-JCH
3 mL (50/box)
6 mL (30/box)
8B-S313-KDG
12 mL (20/box)
Price
Sample Preparation
Specialty Sorbents
54
Part Number
Unit
Price
8B-S013-HBJ
8B-S013-HCH
8B-S013-JCH
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S013-JEG
8B-S013-KDG
8B-S013-LEG
8B-S013-MFF
20 mL (20/box)
12 mL (20/box)
20 mL (20/box)
60 mL (16/box)
Eco-Screen
Melamine
This proprietary normal phase sorbent is topped with sodium sulfate

to remove any excess water and used for the extraction of hydrocarbons from environmental samples, resulting in high recoveries
of naphthalene.
A proprietary phase that allows for the simultaneous extraction of

melamine and cyanuric acid out of food and biological samples.
Sorbent
Format
Mass
Tube
1g
Sorbent
Format
Mass
Tube
100 mg
200 mg
96-Well Plate
50 mg
Part Number
Unit
8B-S046-JBJ
3 mL (50/box)
Price
Part Number
Unit
Price
8B-S049-EBJ
8B-S049-FBJ
3 mL (50/box)
3 mL (50/box)
8E-S049-DGB
2 Plates/Box
Phenomenex

Specialty Sorbents
SDB-L
(styrene-divinylbenzene)
EPH
(Extractable Petroleum Hydrocarbons)
A rugged polymer sorbent that is pH stable from 1-14 and offers

hydrophobic and aromatic selectivity for reversed phase applications.
This specialty normal phase sorbent was developed for the

fractionation of aliphatic and aromatic hydrocarbons from
environmental samples.
O
Si
OH
Si
OH
Unit
8B-S014-EAK
8B-S014-FBJ
8B-S014-FCH
8B-S014-HBJ
8B-S014-HCH
8B-S014-JCH
1 mL (100/box)
3 mL (50/box)
6 mL (30/box)
3 mL (50/box)
6 mL (30/box)
6 mL (30/box)
8B-S014-MFF
60 mL (16/box)
8E-S014-DGB
2 Plates/Box
Price
Part Number
Unit
8B-S031-HBJ
3 mL (50/box)
5g
8B-S031-LEG
20 mL (20/box)
5g
8B-S031-LEG-T
20 mL (20/box)
Price
Giga Tube
Teflon Giga Tube
Part Number
Sorbent
Format
Mass
Tube
500 mg
96-Well Plate
50 mg
PAH
(Polycyclic Aromatic Hydrocarbons)
Sodium Sulfate
This proprietary sorbent was designed to provide high recoveries of

polycyclic aromatic hydrocarbons from water (as specified in EPA
Method 550.1) while simultaneously removing humic acids from the
extract.
A specialized sorbent that is used for the removal of aqueous

residues from organic solutions in an effort to reduce blowdown time.
Sorbent
Format
Mass
Tube
750 mg
1.5 g
Sorbent
Format
Mass
Tube
1g
Part Number
Unit
8B-S130-WCH
8B-S130-7CH
6 mL (30/box)
6 mL (30/box)
Sorbent
Format
Mass
Tube
100 mg
200 mg
200 mg
500 mg
500 mg
1g
Giga Tube
10 g
Price
Part Number
Unit
8B-S124-JCH
6 mL (30/box)
8B-S124-JEG
8B-S124-LEG
20 mL (20/box)
20 mL (20/box)
Price
Giga Tube
1g
5g
Dont see the size or format you want? Contact Phenomenex or

your local distributor for other dimensions, Giga tubes, and bulk
sorbent pricing and part numbers.
Phenomenex
55
Biological Sample Cleanup

Strata Impact
Rapid Protein Precipitation
Quickly cleanup sample by passing biological samples
through the filter in the protein precipitation plate
Increase sensitivity of your analysis by eliminating
proteins which contribute to baseline noise
Increase reproducibility with the leak-free membrane,
preventing premature sample breakthrough and
incomplete protein precipitation
biological sample cleanup | Sample Preparation
Solvent
Shielding
Technology
prevents
leaks
Leak
occurs
Standard
Precipitation Plate
General Protocol
Leak-free
Place
The Strata Impact plate onto a suitable
96-well manifold or robot. Ensure that a
96-well collection plate is positioned inside
the manifold to collect filtrate.
Dispense*
Organic solvent in a volume of 3 4x the
volume of the intended biological sample.
Maximum volume of organic solvent should
not exceed 1.2 mL.
Add
25 L 300 L of biological sample directly
into the organic solvent in each well of the
Strata Impact plate, maintain a final ratio of
3:1 to 4:1 organic solvent:sample.
Vortex
2 minutes at maximum possible speed,
taking care not to allow cross contamination.
Sample can stand for up to 25 minutes.
Filter
Centrifuge at 500 g for 5 minutes
or vacuum at 2 7 inch Hg for up to 3
minutes or until filtrate is collected.
Strata Impact Plate
Can retain acetonitrile with no leaks for

up to 25 minutes
Compatible Solvents
Solvent : Sample Ratio
Acetonitrile
3:1 to 4:1
Methanol
4:1
Strata Impact Precipitation Plates
Part No.
Description
Strata Impact Precipitation Plates
CE0-7565 Strata Impact Protein Precipitation, Square Well,
Filter Plate, 2 mL
Strata Impact Starter Kit for Protein Precipitation
CE0-8201 Strata Impact Protein Precipitation Plate (2 ea)
Collection Plate 2 mL (2 ea)
Sealing Mat, Santoprene (AH0-8199) (2 ea)
Unit/Box
For 96-Well Plate Vacuum Manifold, see p. 58
Price
ea
* A 3:1 v/v ratio of organic solvent to biological sample will dilute your
sample less. In contrast, a 4:1 v/v ratio of organic solvent to biological
sample will ensure a more complete precipitation.
When used with a liquid-handling instrument or automation, aspirate/

dispense cycles may be used to promote in-tip mixing and precipitation.
This will ensure complete precipitation and filtration. Vortexing is not
necessary when in-tip precipitation is performed.
56
Phenomenex

Vacuum Manifolds for Processing Samples
Sample Processing Guidelines for Strata-X Sorbents
Vacuum Manifolds for Tubes
Maximize throughput with simple, cost effective batch processing of up
to 96 samples per hour. (24 samples per 15 minutes)
Fits 13 mm and 16 mm test tubes up to 125 mm in height.
Slow is Safe for loading and elution. A flow rate of 13 drops per second
(1 3 mL /min) is recommended during the loading and elution steps for
typical small volume samples (< 5 mL). At these critical steps
the analytes are chemically interacting with the sorbent.
Large volume samples (> 100 mL) in large cartridges (>1 gram) may be
processed at flow rates between 5 10 mL/minute.
Conditioning and Wash steps are generally not flow critical.
Use an adapter cap to attach another

SPE tube, which can be used to
increase the reservoir size for washing
or eluting solvents.
Teflon Or Vacuum
Resistant Tubing
Adapter
Cap
Vacuum
Manifold
Reversed phase methods are more forgiving of fast flow rates than ionexchange or normal phase.
Have Large Sample Volume but Need

a Small Bed Mass?
Reservoir/Empty
Syringe Barrel
Sample
Individual stopcocks are typically

not needed when using the Strata-X
family of sorbents (Strata-X, X-C, X-CW, X-A,
X-AW, XL, XL-C, XL-CW, XL-A, XL-AW). They are very forgiving of improper
flow rates and are truly resistant to deconditioning effects caused by
excessive drying during the method.
Waste
Trap
Sample Preparation | accessories
Processing Large Sample Volumes
Flow rate is easily adjusted via master

vacuum controller or individual
stopcocks if necessary.
Vacuum
Pump
Adapter Cap
Description
Teflon or Vacuum Resistant Tubing (1/8 inch O.D.)
Adapter Cap
Phenomenex
Part No.
AT0-2956
AH0-7191
(AH0-7191)
57

Vacuum Manifolds
SPE Tube Vacuum Manifold
96-Well Plate Vacuum Manifold
Process up to 12 or 24 samples at one time
Includes vacuum valve attachment and two collection

plate spacer inserts
Process up to 10 large volume samples at one time

Female Luer inlets fit all male Luer tipped SPE tubes and
cartridges
Made of durable acrylic

Designed to accommodate 96-well plates, collection
plates, protein precipitation plates, and filtration plates
Stopcock
Well Plate
Glass Chamber
Collection Rack
Vacuum Gauge
Acrylic Chamber
(manifold top)
accessories | Sample Preparation
Valve Assembly
Manifold Base Plate
(houses collection plate)
Part No.
Description
24 Position Vacuum Manifold*3
AH0-6024
SPE 24-Position Vacuum Manifold Set,
complete assembly
24 Position Vacuum Manifold Replacement Parts
AH0-6026
SPE Glass Chamber
AH0-6028
SPE Cover, Gasket and 24 Stopcocks
AH0-6030
SPE Gaskets
AH0-6038
SPE Collection Rack Assembly, including plates,
legs and clips3
AH0-6049
SPE Luer Stopcocks
12 Position Vacuum Manifold*2
AH0-6023
SPE 12-Position Vacuum Manifold Set,
complete assembly
12 Position Vacuum Manifold Replacement Parts
AH0-6025
SPE 12-Position Glass Chamber
AH0-6027
SPE Cover, Gasket and 12 Stopcocks
AH0-6029
SPE Gaskets
AH0-6037
SPE Collection Rack Assembly, including plates,
legs and clips2
AH0-6052
SPE 12-Position Vacuum Waste Container,
polypropylene
AH0-6049
SPE Luer Stopcocks
10 - Position Tall-Boy Vacuum Manifold*1
AH0-7502
SPE 10-Position Tall-Boy Vacuum Manifold,
complete assembly
10 Position Tall-Boy Vacuum Manifold Replacement Parts
AH0-7503
Glass Chamber
AH0-7504
Cover, Gasket and 10 Stopcocks
AH0-6049
SPE Luer Stopcocks
Unit
ea
ea
ea
2/pk
ea
24/pk
ea
ea
ea
2/pk
ea
10/pk
24/pk
ea
Price
Fully assembled, ready to
cleanup, concentrate, and/or
solvent-switch 96 samples at
one time.
96-Well Plate Manifold**
Part No.
Description
Unit
AH0-8950
Strata 96-Well Plate Manifold,
ea
Universal w/vacuum gauge
Replacement Parts
Part No.
Description
Unit
ea
AH0-7285
96-Well Plate Manifold Replacement Gasket,
Flat (to fit between acrylic chamber and 96well plate), black
ea
AH0-7198
96-Well Plate Manifold Replacement Gasket,
Profile, (to fit between acrylic chamber and
manifold base), white
AH0-8637
Reservoir, Single Well, High Profile, 96 Bottom 25/pk
Troughs
Price
Price
**Manifold, compatible with 2 mL Strata Impact plate, Strata and Strata-X 96-well plate formats.
ea
ea
For additional manifold replacement

parts and accessories, see p. 59
24/pk
* Manifolds include: Vacuum-tight glass chamber, vacuum gauge assembly, polypropylene lid with gasket, male and female luers and yellow
end plugs, stopcock valves, collection rack assemblies, polypropylene needles, lid support legs. Waste container included with 12-position
manifold.
(1) The 10-position Tall Boy Vacuum Manifold Collection Rack includes 4 plates: one base plate, one dimple plate, one small plate and one
large plate and three riser bar legs, along with 12 manifold clips to support the plates. The assembly also includes 10 polypropylene needles,
10 stopcocks and 4 black legs to support the lid when taken off the glass block.
(2) The 12-position Collection Rack Assembly consists of 3 support legs, base plate, dimple plate, small plate, medium plate,
large plate, volumetric plate, and 12 retaining clips.
(3) The 24-position Collection Rack Assembly consists of 3 support legs, base plate, dimple plate, small plate, large plate, and
12 retaining clips.
58
Phenomenex

Collection Plates
Filtration Plate
Available in conical V- and round-bottom formats
Available in 0.45 m membrane porosity
Made of chemically inert polypropylene
Inert surface eliminates non-specific binding for

maximized results
Available in 350 L, 1 and 2 mL volumes
Cost effective solution to meet all filtration goals
Conical V- and round-bottom for maximized

sample delivery
Filtration Plates
Part No.
Description
AF0-8265
0.45 m Glass Fiber 96-Well Filtration Plate
(AH0-8635)
2 mL Square Well
Unit/Box
2
Price
(AH0-7193)
1 mL Square Well
Sealing Mats and Tape
(AH0-7192)
350 L Square Well
(AH0-7279)
1 mL Round Well
Collection Plates*
Part No.
Description
AH0-7192
350 L/well 96-Square Well Conical V-bottom
Collection Plate
AH0-7193
1 mL/well 96-Square Well Conical V-bottom
Collection Plate
AH0-7279
1 mL/well 96-Round Well Round Bottom Collection
Plate
AH0-7194
2 mL/well 96-Square Well Conical V-bottom
Collection Plate
AH0-8635
2 mL/well 96-Square Well Round-Conical Bottom
Collection Plate
AH0-8636
2 mL/well 96-Round Well Round Bottom 8 mm
Collection Plate
Pierceable and Pre-Slit available
Unit
50/pk
Price
50/pk
(AH0-7195)
50/pk
Sealing Mats*
Part No.
Description
AH0-8597
Sealing Mats, Pierceable, 96-Square Well, Silicone
AH0-8598
Sealing Mats, Pre-Slit, 96-Square Well, Silicone
AH0-8631*** Sealing Mats, Pierceable, 96-Round Well 7mm, Silicone
AH0-8632*** Sealing Mats, Pre-Slit, 96-Round Well 7mm, Silicone
AH0-8633** Sealing Mats, Pierceable, 96-Round Well 8mm, Silicone
AH0-8634** Sealing Mats, Pre-Slit, 96-Round Well 8mm, Silicone
AH0-8199
Sealing Mats, Pierceable, 96 Square Well, Santoprene
AH0-7195
Sealing Mats, Pierceable, 96-Square Well, Ethylene
Vinyl Acetate (EVA)
AH0-7362
Sealing Tape Pad
50/pk
50/pk
50/pk
Phenomenex
Price
10/pk
*Square well sealing mats compatible with 2 mL Strata Impact plates, Strata and Strata-X 96-well plates,
and 96 square well collection plates.
Vacuum Manifold Accessories

General Vacuum Manifold Accessories
Part No.
Description
AH0-7191
Adapter Caps for 1, 3 and 6 mL SPE tubes,
polyethylene, with Luer tip
AH0-7379
Adapter Caps for 12, 20, and 60 mL SPE tubes,
polyethylene, with Luer tip
AH0-8278
Strata Syringe and Adapter Kit
AH0-6034
SPE Manifold Needles, polypropylene
AH0-6035
SPE Manifold Needles, stainless steel
AH0-6050
SPE Drying Attachment for 12-position manifold
AH0-6051
SPE Drying Attachment for 24-position manifold
AH0-6053
Female Luer Fittings
AH0-6054
Male Luer Fittings
AH0-6057
Vacuum Gauge and Valve Assembly
AH0-6062
Control Valve, Teflon
AH0-6064
Teflon Needles
AH0-6065
Teflon Needles
Unit
50/pk
50/pk
50/pk
50/pk
50/pk
50/pk
100/pk
50/pk
Sample Preparation | accessories
Fits all Phenomenex 96-well plates, square-well collection

plates, round-well collection plates, protein precipitation
plates, and filtration plates
**8 mm round-well sealing mats compatible with 2 mL round-well 8 mm collection plates (AH0-8636)
***7 mm round-well sealing mats compatible with1 mL round-well 7 mm collection plates (AH0-7279)
Unit
15/pk
Price
6/pk
ea
24/pk
12/pk
ea
ea
2/pk
2/pk
ea
25/pk
100/pk
500/pk
59
Ingenuity, Im
a
gin
30
atio
ng
brati 30 Yea
e
l
e
r
f
so
n, and H
ea
r
We served.
Humanity, that is. In keeping with our mission, we are passionate about
giving back. Each year, we take part in a variety of humanitarian efforts
around the world, spending hours and days building, packing food for
hungry children, logging kilometers for charity, mentoring, donating toys
and goods, raising emergency relief funds, and more, to better the lives
of people in our communities. And we love every single minute of it.
Feed My Starving Children Charity
We were recognized.
Its hard NOT to blush a little (and feel a burst of pride) when achievements that you
have worked so hard for are recognized by your customers and peers. Having loads of
trophies and plaques is never our goal in business, but they certainly do have a way of
reinforcing that we must be heading the right direction in technology, employee welfare,
and giving back.
We created.
Who said science has to be boring, dry, or typical? We
think otherwise! In fact, some of our customers say that
were the coolest separation science company around.
By providing a work atmosphere that encourages creativity and free thinking, we are able to marry science
and art, and bring chromatography to life!
All work and no play makes for a dull company. See page 334.
60
Phenomenex
GC Columns
and
Accessories
GC Column Selection
Column Selection by Manufacturer......................................................... 62

Column Selection Chart........................................................................... 63
Column Selection by EPA Specifications................................................ 64
Column Selection by ASTM Specifications............................................. 65
Column Selection by USP Specifications................................................ 66
GC Columns and Accessories
Essentials for Improving GC Reproducibility
55 - 143
61
138
Integrated Guard Columns, Guardian System......................................... 67

GC Guard Columns and Retention Gaps................................................ 68
Standards, Column Performance............................................................ 98
Essentials for Improved GC Reproducibility:

GC Guard Columns, pp.67-68
Fast GC Columns................................................................................... 69
QC Check Standards, p.98
GC Columns
Zebron Capillary GC Columns
Five (5) Inch Cages.................................................................................. 70

ZB-1, 100% dimethylpolysiloxane.......................................................... 71
ZB-1ms, 100% dimethylpolysiloxane..................................................... 72
ZB-1HT Inferno, 100% dimethylpolysiloxane......................................... 73
ZB-1XT SimDist....................................................................................... 74
ZB-5, 5%-phenyl-95%-dimethylpolysiloxane........................................ 75
SGE Capillary GC Columns
ZB-5MSi, 5%-phenyl-95%-dimethylpolysiloxane.................................. 76
ZB-5ms, 5%-phenyl Arylene-95%-dimethylpolysiloxane...................... 77
forte BPX70, 70% cyanopropyl (equiv.) polysilphenylene-siloxane........ 96
ZB-5HT Inferno, 5%-phenyl-95%-dimethylpolysiloxane.................. 78-79
forte HT8, 8% phenyl (equiv.) polycarborane-siloxane........................... 96
ZB-35, 35%-phenyl-65%-dimethylpolysiloxane.................................... 82
forte SolGel-WAX..................................................................................... 96
ZB-35HT Inferno, 35%-phenyl-65%-dimethylpolysiloxane................... 83
forte BPX90............................................................................................. 96
ZB-50, 50%-phenyl-50%-dimethylpolysiloxane.................................... 84
forte BPX-VOLATILES.............................................................................. 96
ZB-624, 6%-cyanopropylphenyl-94%-dimethylpolysiloxane................ 85
Quadrex Capillary GC Columns

007-225, 50%-cyanopropylphenyl methylpolysiloxane.......................... 97
ZB-1701, 14%-cyanopropylphenyl-86%-dimethylpolysiloxane............ 86
ZB-1701P, 14%-cyanopropylphenyl-86%-dimethylpolysiloxane........... 87
007-502, cyanopropylphenyl methylpolysiloxane................................... 97
ZB-BAC1 and BAC2................................................................................ 95
007-65HT, 65%-phenyl methylpolysiloxane........................................... 97
ZB-Bioethanol.......................................................................................... 91
PLT-5A, molecular sieve 5A PLOT........................................................... 97
ZB-Drug-1................................................................................................ 94
PHAT, Polymer Height Augmentation Technology................................... 97

BTR-CW, polyethylene glycol (PEG) - Oxygen resistant......................... 97
ZB-FFAP, nitroterephthalic acid modified polyethylene glycol................ 90
ZB-MultiResidue (MR)-1..................................................................... 92-93
GC Column Installation Instructions. ..................................... 381-382
ZB-MultiResidue (MR)-2..................................................................... 92-93
Zebron GC Column Applications............................................. 115-143
ZB-MultiResidue Column Kits................................................................. 93
Zebron GC Column MS Certification............................................. 382
ZB-WAX, polyethylene glycol.................................................................. 88
GC Accessories............................................................................... 98-114
ZB-WAXplus, polyethylene glycol............................................................ 89
General Laboratory Consumables and Accessories
ZB-XLB.................................................................................................... 80
Filtration................................................................................................ 9-15
ZB-XLB-HT Inferno.................................................................................. 81
Vials.................................................................................................... 21-34
GC Column Selection by Manufacturer

This section is, neither in terms of manufacturers nor in terms of their products, a complete list, and the accuracy of the data is not guaranteed. Small differences in dimensions or performance might be possible and slight adjustments to your application may be necessary.
Restek
Agilent
Technologies
(J&W)
ZB-1
Rtx-1,
Rtx-1PONA,
Rtx-1 F&F
DB-1,
DB-2887,
DB-1 EVDX
ZB-1ms
Rtx-1MS
DB-1ms
ZB-1HT Inferno
Rxi-1HT
DB-1ht
ZB-1XT SimDist
MXT -1HT SimDist
DB-HT Sim Dis
ZB-5
Rtx-5
DB-5
MDN-5, SPB-5,
PTE-5, SE-54,
PTA-5, Equity-5,
Sac-5
HP-5,
Ultra 2,
HP-PAS-5
ZB-5MSi
Rtx-5MS,
Rtx-5Amine,
Rxi-5ms
DB-5
MDN-5S
HP-5ms,
HP-5msi
ZB-5HT Inferno
Stx-5HT,
XTI-5HT
DB-5ht
HT-5
ZB-5ms
Rtx-5Sil MS
Rxi-5Sil MS
DB-5ms,
DB-5.625,
DB-5ms EVDX
35%-phenyl65%-dimethylpolysiloxane
ZB-35
Rtx-35,
Rtx-35MS
DB-35,
DB-35ms
Proprietary
ZB-35HT Inferno
Composition
100% dimethylpolysiloxane
GC Column Selection by manufacturer | GC COLUMNS & ACCESSORIES
5%-phenyl-Arylene95%-dimethylpolysiloxane
6%-cyanopropyl
phenyl-94%-dimethylpolysiloxane
14%-cyanopropyl
phenyl-86%-dimethylpolysiloxane
14%-cyanopropyl
phenyl-86%-dimethyl
polysiloxane
Polyethylene glycol
Phenomenex
Zebron
ZB-50
Rtx-50
ZB-624
Rtx-1301,
Rtx-624
ZB-1701
Rtx-1701
ZB-1701P
ZB-WAX
DB-17, DB-17HT,
DB-17ms,
DB-17 EVDX
DB-1301,
DB-624,
DB-VRX
DB-1701
Rtx-WAX, Famewax,
DB-WAXetr
Stabilwax-DB
DB-WAX,
Stabilwax
CAM
Varian
(Chrompack)
OV
BP1, BP1-PONA,
BPX1-SimD
CP-Sil 5 CB
OV-1
SolGel-1ms
CP-Sil 5 CB MS,
VF-1ms
Alltech
SGE
HP-1,
HP-101,
HP-PONA, Ultra 1
AT-1,
AT-Sulfur, EC-1
HP-1ms
AT-1ms
Petrocol 2887
CP-SimDist
CP-SimDist
UltiMetal
AT-5,
EC-5
BP5,
BPX5
CP-Sil 8 CB
OV-5
VF-5ht
VF-5ms,
CP-Sil 8 CB MS
MDN-35,
SPB-35,
SPB-608
HP-35,
HP-35ms
AT-35
BPX35,
BPX608
SP-2250,
SPB-17,
SPB-50
HP-50+
AT-50
BPX50
CP-Sil 24 CB
SPB-1301,
SPB-624
HP-VOC
AT-624,
AT-1301
BP624
CP-1301,
CP-Select 624 CB
OV- 624
AT-1701
BP10
CP-Sil 19 CB
OV -1701
SPB-1701,
Equity-1701
OV-11
Met-Wax,
Omegawax
HP-INNOWax
EC-Wax
SolGel-WAX
CP-Wax 57 CB
Supelcowax 10
HP-20M,
Carbowax 20M
AT-Wax,
AT-AquaWax
BP20
CP-Wax 52 CB
Carbowax
20M
DB-FFAP
Nukol,
SPB-1000
HP-FFAP
AT-1000, EC-1000 BP21
CP-Wax 58
(FFAP) CB,
CP-FFAP CB
OV-351
MDN-12
ZB-WAXplus
Nitroterephthalic acid
modified polyethylene
glycol
ZB-FFAP
Stabilwax-DA
MultiResidue-1
(MR-1)
MultiResidue-2
(MR-2)
Rtx-CLPesticides,
Stx-CLPesticides
Rtx-CLPesticides2,
Stx-CLPesticides2
Proprietary
ZB-XLB
Rtx-XLB
DB-XLB
Proprietary
ZB-XLB-HT Inferno
Proprietary
ZB-Drug-1
Proprietary
ZB-BAC1
Rtx-BAC1
DB-ALC1
Proprietary
ZB-BAC2
Rtx-BAC2
DB-ALC2
Proprietary
ZB-Bioethanol
Proprietary
SPB-1, SPB-1 TG,

SE-30, MET-1,
SPB-1 Sulfur,
SPB-HAP
MDN-1,
Equity-1
Agilent
Technologies (HP)
DB-1701P
Polyethylene glycol
Proprietary
Supelco
VF-XMS
For Zebron GC columns, see pp. 61-95
62
Phenomenex
Zebron GC Column Selection Chart
Your Analytes
Non-Polar
Mixed-Polarity
Polar
Semi-Volatile
Volatile
General Analytes
Pesticides
ZB-5ms
ZB-MR-1
ZB-XLB-HT Inferno
ZB-35HT Inferno
ZB-MR-1
ZB-MR-2
ZB-5ms
ZB-XLB-HT Inferno
ZB-35HT Inferno
ZB-624
ZB-WAXPLUS
ZB-WAX
ZB-1*
*Thick Film (1.00 m)
Base
Neutral
Acid
ZB-WAX
ZB-XLB-HT Inferno
ZB-WAX
ZB-50
ZB-35HT Inferno
ZB-XLB-HT Inferno
ZB-MR-2
ZB-FFAP
ZB-XLB-HT Inferno
ZB-MR-2
Volatile
Semi-Volatile
ZB-1*
ZB-5*
PLOT
ZB-1ms
ZB-5ms
ZB-5MSi
ZB-1HT Inferno
ZB-5HT Inferno
ZB-XLB-HT Inferno
ZB-35
ZB-1XT SimDist
*Thick Film (1.00 m)
Phenomenex
Volatile
ZB-624
ZB-WAXPLUS
ZB-WAX
GC COLUMNS & ACCESSORIES | Zebron GC Column selection chart
Semi-Volatile
63
GC Column Selection by epa Specifications

Listed below are a few of our recommended Zebron columns for EPA analyses. Other possible columns can also be used for these analyses.
Please contact Phenomenex for your specific GC column needs.
EPA Drinking Water Test Methods
Method
501.3
502.2
503.1
504.1
505
507
508
513
515.2
524.2
525
551.1
552.2
Description
Trihalomethanes by GC/MS and SIM
Volatile Halogenated Organics in Water by Purge & Trap GC/PID/ELCD
Volatile Aromatics & Unsaturated Organics by Purge & Trap GC
1,2-Dibromoethane (EDB), 1,2-Dibromo-3-chloropropane (DBCP), and 1,2,3-Trichloropropane
(123TCP) by GC/ECD
Organohalide Pesticides & Aroclors by GC/ECD
Nitrogen & Phosphorus Containing Pesticides in Water by GC/NPD
Chlorinated Pesticides in Water by GC/ECD
2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin by GC w/ High Resolution Mass Spec
Determination of Chlorinated Acids in Water Using Liquid-Solid Extraction & GC/ECD
Measurement of Purgeable Organic Compounds in Water by Purge & Trap Capillary Column
GC/MS
Organic Compounds in Drinking Water by Liquid-Solid Extraction and Capillary Column GC/MS
Chlorinated Solvents & Disinfection By-Products in Drinking Water by Liquid-Liquid Extraction
& GC/ECD
Haloacetic Acids & Dalapon in Drinking Water by Ion-Exchange Liquid-Solid Extraction & GC/ECD
Phenomenex Recommendation
ZB-624
ZB-624
ZB-624
ZB-1, ZB-624
Page
85
85
85
71, 85
ZB-1, ZB-MR-1, ZB-MR-2

ZB-MR-1, ZB-MR-2, ZB-35HT, ZB-XLB-HT
ZB-MR-1, ZB-MR-2, ZB-35HT, ZB-XLB-HT
ZB-5ms
ZB-5MSi, ZB-5ms, ZB-1701P, ZB-35HT, ZB-XLB-HT
ZB-624
71, 92-93
92-93, 83, 81
92-93, 83, 81
77
76, 77, 87, 83, 81
85
ZB-5MSi, ZB-5ms
ZB-35
76, 77
82
ZB-5ms, ZB-1701, ZB-35HT, ZB-XLB-HT
77, 86, 83, 81
GC Column Selection by EPA Specifications | GC COLUMNS & ACCESSORIES
EPA Waste Water Test Methods
64
Method
601
602
603
604
606
607
608
609
610
611
612
613
615
619
622
624
625
1618
1624
1625
1653
Description
Purgeable Halocarbons
Purgeable Aromatics
Acrolein & Acrylonitrile
Phenols
Phthalate Esters
Nitrosamines
Organochlorine Pesticides & PCBs
Nitroaromatics & Isophorone
Polynuclear Aromatic Hydrocarbons
Haloethers
Chlorinated Hydrocarbons
2,3,7,8-Tetrachlorodibenzo-p-dioxin
Chlorinated Herbicides
Triazine Herbicides
Organophosphorus Pesticides
Purgeable Volatiles
Base/Neutral and Acids
Organophosphorus Pesticides, Organohalide Pesticides, Phenoxyacid Herbicides
Volatile Organic Compounds by Isotope Dilution GC/MS
Semi-volatile Organic Compounds by Isotope Dilution GC/MS
Chlorinated Phenols in Waste Water by In-Situ Acetylation and GC/MS
ZB-624
ZB-624
ZB-624
ZB-5MSi, ZB-5ms
ZB-5MSi, ZB-5ms
ZB-5MSi, ZB-5ms, ZB-35, ZB-1701P, ZB-XLB-HT
ZB-MR-1, ZB-MR-2, ZB-XLB-HT, ZB-35HT
ZB-5MSi, ZB-5ms
ZB-5ms, ZB-35
ZB-5MSi, ZB-5ms
ZB-5MSi, ZB-5ms
ZB-5ms
ZB-5ms, ZB-5MSi, ZB-XLB-HT, ZB-35HT
ZB-5ms, ZB-50, ZB-MR-1, ZB-MR-2, ZB-5MSi, ZB-XLB-HT
ZB-5ms, ZB-35, ZB-MR-1, ZB-MR-2, ZB-5MSi, ZB-XLB-HT
ZB-624
ZB-5MSi, ZB-5ms , ZB-XLB-HT
ZB-XLB-HT, ZB-MR-1, ZB-MR-2, ZB-35HT
ZB-624
ZB-5ms
ZB-5ms
Page
85
85
85
76, 77
76, 77
76, 77, 82, 87, 81
92-93, 81, 83
76, 77
77, 82
76, 77
76, 77
77
77, 76, 81, 83
77, 84, 92-93, 76, 81
77, 84, 92-93, 76, 81
85
76, 77, 81
81, 92-93, 83
85
77
77
ZB-624
ZB-5MSi, ZB-5HT, ZB-624
ZB-624
ZB-624
ZB-5MSi, ZB-5ms
ZB-5MSi, ZB-5ms
ZB-5MSi, ZB-5ms, ZB-35HT, ZB-XLB-HT, ZB-MR-1, ZB-MR-2
ZB-35HT, ZB-5MSi, ZB-XLB-HT, ZB-MR-1, ZB-MR-2
ZB-5ms
ZB-5MSi, ZB-5ms, ZB-35
ZB-XLB-HT,ZB-5ms, ZB-5MSi, ZB-MR-1, ZB-MR-2
ZB-XLB-HT, ZB-35HT, ZB-5MSi, ZB-MR-1, ZB-MR-2
ZB-XLB-HT, ZB-5ms, ZB-35, ZB-MR-1, ZB-MR-2
ZB-624
ZB-5MSi, ZB-5ms, ZB-XLB-HT
ZB-5MSi, ZB-5ms
Page
85
76, 78-79, 85
85
85
76, 77
76, 77
76, 77, 83, 81, 92-93
83, 76, 81, 92-93
77
76, 77, 82
81, 77, 76, 92-93
81, 83, 76, 92-93
81, 77, 82, 92-93
85
76, 77, 81
76, 77
EPA Solid Waste Test Methods

Method
8010B
8015C
8021B
8030A
8041A
8061A
8081B
8082A
8091
8100
8121
8141B
8151A
8260B
8270D
8280B
Description
Halogenated Volatile Organics
Nonhalogenated Volatile Organics
Aromatic and Halogenated Volatiles
Acrolein, Acrylonitrile, Acetonitrile
Phenols
Phthalate Esters
Organochlorine Pesticides
Polychlorinated Biphenyls (PCBs) by GC ECD
Nitroaromatics and Cyclic Ketones
Chlorinated Hydrocarbons
Organophosphorus Pesticides
Chlorinated Herbicides
GC/MS for Volatile Organics: Capillary Column Techniques
GC/MS for Semi-volatile Organics: Capillary Column Techniques
Analysis of Polychlorinated Dibenzo-p-dioxins and Polychlorinated Dibenzofurans
Phenomenex
GC Column Selection by ASTM Specifications

Listed below are a few of our recommended Zebron columns for ASTM methods. Other possible columns can also be used for these
analyses. Please contact Phenomenex for your specific GC column needs.
ASTM Methods
Description
Natural gas
Reformed gas
FAME analysis
Oils and oil acids in solvent-reducible paints
Analysis of n-heptane and iso-octane (high purity)
Xylene isomers
Butadiene and styrene in butadiene concentrates
Non-condensable gases in C1-C3 hydrocarbons
Phenols in water
Aromatic traces in light saturated hydrocarbons
Oil and oil acids
FAME analysis
Purity of methyl ethyl ketone
SimDist analysis of petroleum fractions
Volatile organics in water
Polyhydric alcohols in alkyd resins
Monopentaerythritol in commercial pentaerythritol
Composition of turpentine
Purity and benzene content of cyclohexane
Organochlorine pesticides in water
Polymers in emulsion paints
Solvent analysis in paints
PCBs in environmental materials
Comparison of waterborne petroleum oils
Purity of methyl isobutyl ketone
Total hydrocarbons, methane and CO in air
Toluene diisocyanates in urethane prepolymers
Purity of trichlorotrifluoroethane (CFC-113)
Identification of rubber
FAME analysis
Purity of monomeric plasticizers
Diesel fuel in lubricating oil (SAE 30)
PCBs in water
Benzene and toluene in gasoline
Volatile organic compounds
Gasoline fractions
Fatty acids in drying oils
Analysis of cumene
Analysis of o-xylene
Analysis of p-xylene impurities
Methoxyl and hydroxypropyl substitution in cellulose ether products
Impurities in styrene
PCBs in insulating liquids
Butylated hydroxy toluene in ethylene and ethylenevinylacetate polymers
Benzene in hydrocarbon solvent
Aromatics in gasoline
Thiophene impurities in benzene
Phenol and cresol inhibitors in insulating oils
Impurities in ethylbenzene
Petroleum naphthas through n-nonane
Analysis of styrene
Determination of denatured bioethanol
Aromatics in finished gasoline
Extended SimDist
Determination of glycerine in biodiesel
Crude Oil; Vacuum distillates
Analysis of glycols
Analysis of denaturated ethanol
Phenomenex
PLT-5A
PLT-5A, ZB-1
BPX70
BPX70
ZB-1
ZB-WAX, ZB-WAXplus
ZB-1
PLT-5A, ZB-1*
ZB-5, ZB-5MSi
ZB-WAX
BPX70
BPX70
ZB-WAX
ZB-1HT, ZB-1XT SimDist
ZB-WAX, ZB-WAXplus
ZB-1
ZB-1
ZB-WAXplus
ZB-1
ZB-5, ZB-5MSi, ZB-MR-1, ZB-MR-2
ZB-1
ZB-WAXplus
ZB-5, ZB-5MSi, ZB-MR-1, ZB-MR-2
ZB-1
ZB-WAXplus
PLT-5A
ZB-1
ZB-1, ZB-624
ZB-1HT
BPX70
ZB-1
ZB-1HT
ZB-5, ZB-5MSi
ZB-1
ZB-WAX, ZB-WAXplus
ZB-1XT SimDist
ZB-FFAP
ZB-WAX, ZB-WAXplus
ZB-WAX, ZB-WAXplus
ZB-WAX, ZB-WAXplus
ZB-1
ZB-FFAP
ZB-5MSi, ZB-5HT
ZB-1
ZB-1
ZB-1
ZB-FFAP
ZB-FFAP
ZB-FFAP, ZB-WAX, ZB-WAXplus
ZB-1
ZB-WAX, ZB-WAXplus
ZB-1, ZB-Bioethanol
ZB-1
ZB-1HT, ZB-1XT SimDist
ZB-5HT
ZB-1XT SimDist
ZB-WAXplus, ZB-1
ZB-WAXplus, ZB-Bioethanol
*Thick phase
Page
97
97, 71
96
96
71
88, 89
71
97, 71
75, 76
88
96
96
88
73, 74
88, 89
71
71
89
71
75, 76, 92-93
71
89
75, 76, 92-93
71
89
97
71
71, 85
73
96
71
73
75, 76
71
88, 89
74
90
88, 89
88, 89
88, 89
71
90
76, 78-79
71
71
71
90
90
90, 88, 89
71
88, 89
71, 91
71
73, 74
78-79
74
89, 71
89, 91
GC COLUMNS & ACCESSORIES | GC Column Selection by ASTM Specifications
Method
D 1945
D 1946
D 1983
D 2245
D 2268
D 2306-96
D 2426
D 2504
D 2580
D 2600
D 2743
D 2800
D 2804
D 2887
D 2908
D 2998
D 2999
D 3009
D 3054
D 3086
D 3168
D 3271
D 3304
D 3328
D 3329
D 3416
D 3432
D 3447
D 3452
D 3457
D 3465
D 3524
D 3534
D 3606
D 3687
D 3710
D 3725
D 3760
D 3797
D 3798
D 3876
D 3962
D 4059
D 4275
D 4367
D 4420
D 4735
D 4768
D 5060
D 5134
D 5135-95
D 5501
D 5580
D 6352
D 6584
D 7169
E 0202
E 1100
65
GC Column Selection by USP Specifications

Listed below are a few of our recommended GC columns for USP methods. Other possible columns can also be used for these analyses.
Please contact Phenomenex for your specific GC column needs.
GC Column Selection by USP Specifications | GC COLUMNS & ACCESSORIES
USP Column Classification
66
USP
G1
G2
G3
G5
G7
G14
G15
G16
G17
G19
G20
G25
G27
G27
G28
G32
G35
G36
G38
G39
G41
G42
G43
G46
G48
Phase Composition
Dimethylpolysiloxane oil
Dimethylpolysiloxane gum
50% Phenyl-50% methylpolysiloxane
3-Cyanopropylpolysiloxane
50% 3-Cyanopropyl-50% phenylmethylsilicone
Polyethylene glycol (average MW 950-1,050)
Polyethylene glycol (average MW 3,000-3,700)
Polyethylene glycol (average MW 15,000)
25% Phenyl-25% cyanopropylmethylsilicone
Polyethylene glycol (average MW of 380-420)
Polyethylene glycol TPA (Carbowax 20M terephthalic acid)
5% Phenyl-Arylene-95% methylpolysiloxane
20% Phenylmethyl-80% dimethylpolysiloxane
Polyethylene glycol & diepoxide esterified with nitroterephthalic acid
1% Vinyl-5% phenylmethylpolysiloxane
Phase G1 plus a tailing inhibitor
Polyethylene glycol (average MW 1,500)
Phenylmethyldimethylsilicone (10% phenyl substituted)
35% Phenyl-65% dimethylpolysiloxane
6% Cyanopropylphenyl-94% dimethylpolysiloxane
14% Cyanopropylphenyl-86% methylpolysiloxane
Highly polar, partially cross-linked cyanopolysiloxane
ZB-1, ZB-1ms, ZB-1HT
ZB-50
BPX70
007-225
ZB-WAX, ZB-WAXplus
ZB-WAX, ZB-WAXplus
ZB-WAX, ZB-WAXplus
ZB-50
007-225
ZB-WAX, ZB-WAXplus
ZB-FFAP
ZB-5, ZB-5MSi, ZB-5HT
ZB-5ms
ZB-35, ZB-35HT
ZB-35, ZB-35HT
ZB-FFAP
ZB-WAX, ZB-WAXplus
ZB-35, ZB-35HT
ZB-624
ZB-1701, ZB-1701P
BPX70
Page
71, 72, 73
71, 72, 73
84
96
97
88, 89
88, 89
88, 89
84
97
88, 89
90
75, 76, 78-79
77
82, 83
82, 83
90
75, 76, 78-79
71, 72, 73
88, 89
75, 76, 78-79
82, 83
85
86, 87
96
Phenomenex
Zebron Guardian: Integrated Guard Columns
Guardian System
Out-of-box, Built-in Column Protection
Minimal effect on chromatography
Guardian columns have the 2m, 5 m or 10 m guard built directly

into the analytical column in one continuous length of tubing. Unlike
traditional guard columns, which are known to be difficult to seal and
prone to leaking after normal column maintenance, the Guardian
system provides the same inert column protection, but eliminates
the possibility of leaks.
Quick set-up and simple installation

Complete deactivation
Eliminates the potential for leaks
Extends column life
Improves analyte focusing for low boiling compounds
Aggressively tested to ensure deactivation
No Leaks, No Worries!
The Guardian system is excellent for dirty samples that rapidly

degrade GC column performance. The Guardian system allows
routine column maintenance to be performed without worrying about
leaks or having to update retention times when the column is cut.
The worry-free aspect of the Guardian system makes it an
essential.
Guardian
Should you be using a guard column?
Continuous deactivation for better

peak shape and inertness
If you are working with samples that contain unknown impurities, chances
are you should be using a guard. Guard columns help protect your expensive
analytical column by:
1. Trapping non-volatiles that would otherwise deposit on the column
2. Prevent acidic and/or basic matrix compounds from stripping the
stationary phase
VS.
Traditional Union Connected
Guard Column
3. Reduces retention time shifts due to column maintenance.
Potential for leaks
Bottom line, if you want longer column life, you should be using a
guard column!
Increased activity
Hassle to install
Guardian: Integrated Guard Columns
GC Column Phase
Dimensions
ZB-1ms
Zebron ZB-1ms
15 meter x 0.25 mm x 0.25 df(m)
Zebron ZB-1ms
30 meter x 0.25 mm x 0.25 df(m)
ZB-1HT
Zebron ZB-1HT
30 meter x 0.25 mm x 0.10 df(m)
ZB-5ms
Zebron ZB-5ms
15 meter x 0.25 mm x 0.25 df(m)
Zebron ZB-5ms
30 meter x 0.25 mm x 0.25 df(m)
Zebron ZB-5ms
30 meter x 0.25 mm x 0.50 df(m)
Zebron ZB-5ms
30 meter x 0.32 mm x 0.25 df(m)
Zebron ZB-5ms
30 meter x 0.32 mm x 1.00 df(m)
ZB-5
Zebron ZB-5
30 meter x 0.25 mm x 0.25 df(m)
Zebron ZB-5
30 meter x 0.25 mm x 0.50 df(m)
Zebron ZB-5
60 meter x 0.25 mm x 0.25 df(m)
ZB-5HT
Zebron ZB-5HT
30 meter x 0.25 mm x 0.10 df(m)
Zebron ZB-5HT
30 meter x 0.25 mm x 0.25 df(m)
Zebron ZB-5MSi
Zebron ZB-5MSi
20 meter x 0.18 mm x 0.18 df(m)
Zebron ZB-5MSi
30 meter x 0.25 mm x 0.1 df(m)
Zebron ZB-5MSi
30 meter x 0.25 mm x 0.25 df(m)
Zebron ZB-50
Zebron ZB-50
30 meter x 0.25 mm x 0.25 df(m)
Zebron ZB-MultiResidue-1
Zebron ZB-MultiResidue-1 30 meter x 0.25 mm x 0.25 df(m)
Zebron ZB-SemiVolatiles
Zebron ZB-SemiVolatiles
30 meter x 0.25 mm x 0.25 df(m)
2 m Guardian Part No.
Price
7HG-G011-11-GGA
7HG-G014-02-GGA
7HG-G010-11-GGA
7HG-G010-17-GGA
7HM-G010-11-GGA
7HM-G010-22-GGA
Phenomenex
Price
7EG-G011-11-GGC
7HG-G011-11-GGC
7EG-G010-11-GGC
7HG-G010-11-GGC
7HG-G010-17-GGC
7HG-G002-11-GGA
7HG-G002-17-GGA
7KG-G002-11-GGA
7HG-G002-11-GGC
7HG-G002-17-GGC
7HG-G015-02-GGA
7HG-G015-11-GGA
7FD-G018-08-GGA
7HG-G018-02-GGA
7HG-G018-11-GGA
7CD-G004-08-GGT
Inquire about adding a FREE Guardian when you order

5 GC columns or more.
Price
7HG-G016-11-GGC
7HG-G027-11-GGA
auf Anfrage
7HG-G027-11-GGC
GC COLUMNS & ACCESSORIES | Zebron Guardian: Integrated Guard Columns
Seamless guard transition,

no connection hassles and no
potential for leaks
auf Anfrage
For useful GC accessories, see pp. 98-114
67
GC Guard Columns and Retention Gaps

Z-Guard Columns
INDIVIDUALLY
QC TESTED
Protect and Extend Column Lifetime

Individually QC tested to ensure the highest level of
quality
Extend column lifetime by preventing stationary phase
damage
Improve separation and peak shapes (especially early
elutors)
Improve sensitivity and accuracy of quantitative results
Available as individual guard columns or as complete
kits with connectors
GC Guard Columns and Retention Gaps | GC COLUMNS & ACCESSORIES
To ensure that all Z-Guards are the highest possible quality, we individually test each one! The columns are attached to a reference
Zebron ZB-5 column and are tested using our specially designed
QC mix. We carefully monitor activity, bleed, and stability. This way,
we are able to say with confidence that Z-Guards will provide the
low activity and high quality your methods require.
Z-Guard Columns will trap non-volatile residues and prevent them
from contaminating your expensive analytical column. When you
must cut the end off the guard column to remove contaminant
High Temperature Guard Columns and Kits
ID (mm)
Description
Part No.
5-Meter
0.25
Guard Column
7AG-G000-00-GH0
Guard Column Kit
7AG-G000-00-GHK
0.32
Guard Column
7AM-G000-00-GH0
Guard Column Kit
7AM-G000-00-GHK
0.53
Guard Column
7AK-G000-00-GH0
Guard Column Kit
7AK-G000-00-GHK
Guard Columns and Kits
ID (mm)
Description
0.10
0.18
0.20
0.25
0.32
0.53
Guard Column
Guard Column Kit
Guard Column
Guard Column Kit
Guard Column
Guard Column
Guard Column Kit
Guard Column
Guard Column Kit
Guard Column
Guard Column Kit
Bulk Z-Guard Columns

ID (mm)
Description
50-Meter
0.25
Guard Column
0.32
Guard Column
0.53
Guard Column
10-Meter
0.32
Guard Column
5-Meter
0.53
Guard Column
Part No.
5-Meter
7AB-G000-00-GZ0
7AB-G000-00-GZK
7AD-G000-00-GZ0
7AD-G000-00-GZK
7AE-G000-00-GZ0
7AG-G000-00-GZ0
7AG-G000-00-GZK
7AM-G000-00-GZ0
7AM-G000-00-GZK
7AK-G000-00-GZ0
7AK-G000-00-GZK
Part No.
Price
Part No.
10-Meter
7CG-G000-00-GH0
7CG-G000-00-GHK
7CM-G000-00-GH0
7CM-G000-00-GHK
7CK-G000-00-GH0
7CK-G000-00-GHK
Price
Price
Part No.
10-Meter
7CB-G000-00-GZ0
7CB-G000-00-GZK
7CD-G000-00-GZ0
7CD-G000-00-GZK
7CG-G000-00-GZ0
7CG-G000-00-GZK
7CM-G000-00-GZ0
7CM-G000-00-GZK
7CK-G000-00-GZ0
7CK-G000-00-GZK
Price
Unit
7JG-G000-00-GZ0
7JM-G000-00-GZ0
7JK-G000-00-GZ0
ea
ea
ea
7CM-G000-00-GZ1
10/pk
7AK-G000-00-GZ1
10/pk
Multi-Pak ZB-5 Z-Guard Column

ID (mm)
Description
2-Meter
0.25
Zebron ZB-5 Z-Guard Column
68
buildup, the integrity of your analytical column remains intact. The

fused silica tubing of the guard column is without stationary phase
and deactivated to provide minimal peak distortion and good wettability for polar as well as nonpolar compounds. The lifetime of
your analytical column can be significantly increased by the use of
a guard column.
Part No.
Unit
KG0-7868
25/pk
Price
Universal GC Guard Column. Designed for use

with virtually any GC Capillary column from
virtually any manufacturer. Alternative to: J&W,
Restek, Supelco, Agilent Technologies (HP),
Alltech, Varian (Chrompack) and many more.
Z-Guard Column Kits include 5 or 10 meters
of deactivated fused silica tubing, 5 universal
connectors and 0.5 mL of high-temperature
polyimide resin.
For useful GC accessories, see pp. 98-114
Replacement Parts for Z-Guard Kits

Description
Universal Capillary Column Union,
Borosilicate
High Temperature Polyimide Resin, 0.5 mL
Part No.
Unit
Price
AG0-4716
5/pk
AG0-8514
ea
Price
Phenomenex
Zebron Fast GC Columns
If Zebron columns do not provide you

with equivalent or better separations as
compared to any other GC column of the
same phase and comparable dimensions,
return the column with comparative data
Fast GC Columns
Increased lab productivity and
profitability
Decreased run times

Improved resolution and sensitivity
Increased throughput without the

need to buy any new GC and GC/MS
instrumentation
Minimal method change with equivalent

elution order
Polynuclear Aromatic Hydrocarbons: EPA Method 8100
Increase throughput with

Fast GC columns
6 7
5
4
13
16
13
15
14
10
12
14
16
18
min
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-5ms
30 meter x 0.25 mm x 0.25 m
7HG-G010-11
Split 15:1 @ 310C, 1 L
Helium @ 1.4 mL/min (constant flow)
170C to 240C @ 15C/min to 275C @ 4C/min to
320C @ 10C/min for 5 min
Detector: FID @ 330C
Sample: 1. Naphthalene
10. Benz[a]anthracene
2. 2-Methylnaphthalene 11. Chrysene
3. Acenaphthalene
12. Benzo[b]fluoranthene
4. Acenaphthene
13. Benzo[k[fluoranthene
5. Fluorene
14. Benzo[a]pyrene
6. Phenanthrene
15. Indeno[1,2,3-cd]pyrene
7. Anthracene
16. Dibenz[a,h]anthracene
8. Fluoranthene
17. Benzo[g,h,i]perylene
9. Pyrene
Zebron ZB-5ms
10 meter x 0.10 mm x 0.10 m
7CB-G010-02
Split 20:1 @ 320C, 1 L
100C to 200C @ 33C/min to 320C @ 25C/min
FID @ 330C
1. Naphthalene
3. Acenaphthalene
4. Acenaphthene
5. Fluorene
14. Benzo[a]pyrene
6. Phenanthrene
7. Anthracene
8. Fluoranthene
9. Pyrene
Zebron ZB-1HT
Zebron ZB-5
Zebron ZB-5ms
Zebron ZB-5MSi
Zebron ZB-5HT
Zebron ZB-35
Zebron ZB-35HT
Zebron ZB-50
Zebron ZB-624
Zebron ZB-XLB
Zebron ZB-XLB-HT
Zebron ZB-WAX
Zebron ZB-WAXplus
Zebron ZB-MultiResidue-1
Zebron ZB-Drug-1
Phenomenex
Length
10-Meter
10-Meter
20-Meter
20-Meter
20-Meter
20-Meter
10-Meter
20-Meter
20-Meter
20-Meter
20-Meter
10-Meter
20-Meter
10-Meter
20-Meter
10-Meter
20-Meter
10-Meter
20-Meter
10-Meter
20-Meter
10-Meter
20-Meter
20-Meter
10-Meter
ID (mm)
0.10
0.18
0.18
0.18
0.18
0.18
0.18
0.10
0.18
0.18
0.18
0.18
0.18
0.10
0.18
0.10
0.18
0.18
0.18
0.10
0.18
0.10
0.18
0.18
0.18
df (m)
0.10
0.18
0.18
1.00
0.18
0.18
0.18
0.10
0.18
0.32
0.36
0.18
0.18
0.10
0.18
0.10
1.00
0.18
0.18
0.10
0.18
0.10
0.18
0.18
0.18
Temp. Limits (C)

-60 to 360/370
-60 to 360/370
-60 to 360/370
-60 to 360/370
-60 to 400/430
-60 to 360/370
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 360/370
-60 to 400/430
40 to 340/360
40 to 400
40 to 320/340
-20 to 260
30 to 340/360
30 to 400
40 to 250/260
40 to 250/260
20 to 250/260
20 to 250/260
-60 to 320/340
40 to 320/340
Part No.
7CB-G011-02
7CD-G011-08
7FD-G011-08
7FD-G011-22
7FD-G014-08
7FD-G002-08
7CD-G010-08
7CB-G010-02
7FD-G010-08
7FD-G010-51
7FD-G010-53
7CD-G018-08
7FD-G015-08
7CB-G003-02
7FD-G025-08
7CB-G004-02
7FD-G005-22
7CD-G019-08
7FD-G024-08
7CB-G007-02
7FD-G007-08
7CB-G013-02
7FD-G013-08
7FD-G016-08
7CD-G023-08
Price
min
GC COLUMNS & ACCESSORIES | ZebroN Fast GC Columns
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Fast GC Columns
GC Column Phase
Zebron ZB-1ms
17
17
12
16
15
8 min Analysis!
App ID 16017
10
14
App ID 15806
11
11
10
12
69
Zebron GC Columns
Zebron GC Columns on a 5 Inch Cage

Did you know?
All Zebron columns are available on a 5 inch cage. If you need
a 5 inch cage, simply add a (-B) after the part number, e.g.,
7HG-G011-11-B.
Note: Some exceptions may apply. Agilent 6850 and some SRI and process GC systems use
only 5 in. cages.
ZEBRON GC Columns | GC COLUMNS & ACCESSORIES
Zebron GC Columns on a 5 Inch Cage
GC Column Phase
Length
Zebron ZB-1
30-meter
30-meter
30-meter
30-meter
30-meter
Zebron ZB-1HT
30-meter
30-meter
Zebron ZB-35
30-meter
Zebron ZB-5
30-meter
60-meter
Zebron ZB-5HT
15-meter
15-meter
30-meter
30-meter
Zebron ZB-5MS
30-meter
30-meter
Zebron ZB-5MSi
30-meter
30-meter
Zebron ZB-624
30-meter
30-meter
60-meter
Zebron ZB-FFAP
30-meter
30-meter
Zebron ZB-WAX
15-meter
30-meter
30-meter
Zebron ZB-WAXplus
30-meter
30-meter
30-meter
30-meter
ID (mm)
0.25
0.32
0.53
0.53
0.53
0.25
0.32
0.25
0.32
0.25
0.25
0.32
0.25
0.32
0.25
0.25
0.25
0.32
0.32
0.53
0.32
0.32
0.53
0.25
0.25
0.53
0.25
0.32
0.32
0.53
df (m)
1.00
0.25
0.50
3.00
5.00
0.25
0.25
0.25
1.00
0.25
0.10
0.10
0.25
0.25
0.25
1.00
0.25
0.25
1.80
3.00
1.80
0.50
1.00
0.25
0.25
1.00
0.25
0.25
0.50
1.00
Temp. Limits (C)

-60 to 340/360
-60 to 360/370
-60 to 360/370
-60 to 340/360
-60 to 340/360
-60 to 400/430
-60 to 400/430
40 to 340/360
-60 to 340/360
-60 to 360/370
-60 to 400/430
-60 to 400/430
-60 to 400/430
-60 to 400/430
-60 to 325/350
-60 to 325/350
-60 to 360/370
-60 to 360/370
-20 to 260
-20 to 260
-20 to 260
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
20 to 250/260
20 to 250/260
20 to 250/260
20 to 230/240
Part No.
7HG-G001-22-B
7HM-G001-11-B
7HK-G001-17-B
7HK-G001-36-B
7HK-G001-39-B
7HG-G014-11-B
7HM-G014-11-B
7HG-G003-11-B
7HM-G002-22-B
7KG-G002-11-B
7EG-G015-02-B
7EM-G015-02-B
7HG-G015-11-B
7HM-G015-11-B
7HG-G010-11-B
7HG-G010-22-B
7HG-G018-11-B
7HM-G018-11-B
7HM-G005-31-B
7HK-G005-36-B
7KM-G005-31-B
7HM-G009-17-B
7HK-G009-22-B
7EG-G007-11-B
7HG-G007-11-B
7HK-G007-22-B
7HG-G013-11-B
7HM-G013-11-B
7HM-G013-17-B
7HK-G013-22-B
Price
Contact Phenomenex or your local Phenomenex distributor

for additional GC products and applications.
Extend column lifetime. Add a Z-guard

to your next Zebron GC order.
70
Phenomenex
Zebron GC Columns

ZB-1
Column Profile
Low polarity phase suited for true boiling point

compounds
Low
Low bleed (MS Certified), low activity, and high efficiency

Excellent resolving power of critical pairs in complex
petrochemical samples
Temperature Limits
Stability
Applications
Equivalent to USP Phase G2
Temperature Limits: -60 to 360/370C

(Isothermal/TPGC)*
Alternative to Any 100% Dimethylpolysiloxane Phase:
DB-1
HP-PONA
DB-2887
DB-1 EVDX
Rtx-1
HP-1
HP-101
AT-1
Rtx-1PONA
Rtx-1 F&F
MET-1
Ultra 1
BP1
SPB-1
CP-Sil 5 CB
SE-30
007-1
OV-1
2
3
6
9 10 11 1213 14
15
16
17
18
App ID 14943
19
20
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
10
15
20
Drugs of abuse
Essential oils
Hydrocarbons
Natural gas odorants
Pesticides
Mercaptans
Sulfur compounds
(light)
Solvent impurities
Simulated distillation
Semi-volatiles
25 min
Zebron ZB-1
10 meter x 0.53 mm x 2.65 m
7CK-G001-35
Split 10:1 @ 360C, 1 L
35C to 360C @ 15C/min for 5 min
FID @ 360C
1. Pentane (C5)
11. Hexadecane (C16)
2. Hexane (C6)
12. Heptadecane (C17)
3. Heptane (C7)
13. Octadecane (C18)
14. Eicosane (C20)
4. Octane (C8)
5. Nonane (C9)
15. Tetracosane (C24)
16. Octacosane (C28)
6. Decane (C10)
7. Undecane (C11)
17. Dotriacontane (C32)
8. Dodecane (C12)
18. Hexatriacontane (C36)
19. Tetracontane (C40)
9. Tetradecane (C14)
10. Pentadecane (C15)
20. Tetratetracontane (C44)
Engineered Self Crosslinking (ESC) polymer technology.

Zebron GC Columns MS Certification, see p. 382
*Thicker films (1.0 m df) are rated to 340/360 C
(Isothermal/TPGC).
Zebron ZB-1 GC Columns

ID(mm)
df(m)
10-Meter
0.53
2.65
15-Meter
0.25
0.10
0.25
0.25
0.25
1.00
0.32
0.25
0.32
1.00
0.53
0.15
0.53
0.50
0.53
1.50
30-Meter
0.25
0.10
0.25
0.25
0.25
0.50
0.25
1.00
0.32
0.25
0.32
0.50
0.32
1.00
0.32
3.00
0.32
5.00
0.53
0.50
0.53
1.50
0.53
3.00
0.53
5.00
50-Meter
0.25
0.50
60-Meter
0.25
0.25
0.25
1.00
0.32
0.25
0.32
1.00
0.32
3.00
0.32
5.00
0.53
1.50
100-Meter
0.25
0.50
Temp. LimitsC
Part No.
-60 to 340/360
7CK-G001-35
-60 to 360/370
-60 to 360/370
-60 to 340/360
-60 to 360/370
-60 to 340/360
-60 to 360/370
-60 to 360/370
-60 to 340/360
7EG-G001-02
7EG-G001-11
7EG-G001-22
7EM-G001-11
7EM-G001-22
7EK-G001-05
7EK-G001-17
7EK-G001-28
-60 to 360/370
-60 to 360/370
-60 to 360/370
-60 to 340/360
-60 to 360/370
-60 to 360/370
-60 to 340/360
-60 to 340/360
-60 to 340/360
-60 to 360/370
-60 to 340/360
-60 to 340/360
-60 to 340/360
7HG-G001-02
7HG-G001-11
7HG-G001-17
7HG-G001-22
7HM-G001-11
7HM-G001-17
7HM-G001-22
7HM-G001-36
7HM-G001-39
7HK-G001-17
7HK-G001-28
7HK-G001-36
7HK-G001-39
-60 to 360/370
7JG-G001-17
-60 to 360/370
-60 to 340/360
-60 to 360/370
-60 to 340/360
-60 to 340/360
-60 to 340/360
-60 to 340/360
7KG-G001-11
7KG-G001-22
7KM-G001-11
7KM-G001-22
7KM-G001-36
7KM-G001-39
7KK-G001-28
-60 to 360/370
7MG-G001-17
Price
GC COLUMNS & ACCESSORIES | ZebroN GC Columns
Amines
Ethanol
Gases (refinery)
MTBE
Oxygenates and GROs
PCBs
Simulated Distillation Markers: ASTM Method D 2887
Bleed
Used for fingerprinting and routine quality control

analyses (e.g., citrus oils)
Polarity
High
Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G001-11-B. Some
exceptions may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages.
See p. 70
Contact Phenomenex or your local Phenomenex distributor for

additional GC products and applications.

Phenomenex
ZB-1 Test Mix

Part No.: AG0-5155
Price:
71
Zebron GC Columns
ZB-1ms
Column Profile
Very low bleed (MS Certified) phase especially suited to

high sensitivity GC/MS

Low
Extremely inert for active compounds such as drugs,

pesticides, or acids and bases
Polarity
High
Bleed
Improved signal-to-noise ratio for better sensitivity and

mass spectral integrity
Temperature Limits
Stability
Identical selectivity to the ZB-1

Applications

(Isothermal/TPGC)
Amines
Acids
Diesel Fuel
Polychlorinated
Biphenyls (EPA Method
1668)
Drugs of abuse
Flavors & Fragrances
Pesticides
Alternative to Any MS-Certified 100% Dimethylpolysiloxane Phase:

DB-1ms
HP-1ms
AT-1ms
MDN-1
Rtx-1MS
VF-1ms
SOLGEL-1ms
Equity-1
CP-Sil 5 CB ms
Activity is a key measure of column quality. Zebron ZB-1ms columns

are aggressively tested to ensure full deactivation. Below is an
example of the aggressive QC test mix we use, notice the low tailing
for even the most active compounds like 2-Ethylhexanoic Acid!
Test Conditions for Zebron ZB-1ms

4
1
2
3
7
8
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-1ms
30 meter x 0.25 mm x 0.25 m
7HG-G011-11
Split Flow 100 mL/min @ 250C, 1.0 L
Hydrogen @ 1.18 mL/min (constant flow)
140C (Isothermal)
FID @ 325C
1. Decane
2. 2-Ethylhexanoic Acid
3. 4-Chlorophenol
4. Naphthalene
5. Tridecane
6. 1-Undecanol
7. Dicyclohexylamine
8. Pentadecane
App ID 15545
Lower Overall Column Activity
10 min
Zebron ZB-1ms GC Columns

ID(mm)
df(m)
10-Meter
0.10
0.10
0.18
0.18
12-Meter
0.20
0.33
15-Meter
0.25
0.25
0.32
0.25
20-Meter
0.18
0.18
0.18
1.00
25-Meter
0.20
0.33
30-Meter
0.25
0.10
0.25
0.25
0.25
0.50
0.25
1.00
0.32
0.25
0.32
1.00
0.53
1.00
60-Meter
0.25
0.25
0.25
1.00
0.32
1.00
Temp. LimitsC
Part No.
-60 to 360/370
-60 to 360/370
7CB-G011-02
7CD-G011-08
-60 to 360/370
7DE-G011-14
-60 to 360/370
-60 to 360/370
7EG-G011-11
7EM-G011-11
-60 to 360/370
-60 to 360/370
7FD-G011-08
7FD-G011-22
-60 to 360/370
7GE-G011-14
-60 to 360/370
-60 to 360/370
-60 to 360/370
-60 to 360/370
-60 to 360/370
-60 to 360/370
-60 to 360/370
7HG-G011-02
7HG-G011-11
7HG-G011-17
7HG-G011-22
7HM-G011-11
7HM-G011-22
7HK-G011-22
-60 to 360/370
-60 to 360/370
-60 to 360/370
7KG-G011-11
7KG-G011-22
7KM-G011-22
Price
See p. 70
ZB-1ms Test Mix Part No.: AG0-7805

Price:


72
Phenomenex
Zebron GC Columns
2007 R&D 100

Award Recipient
ZB-1HT Inferno
Column Profile
First non-metal columns stable to 430C

Low
Provides true boiling point separation for hydrocarbon

distillation methods
High
Polarity
Longer lifetime with rugged high temperature, polyimide

coated, fused silica tubing
Bleed
Temperature Limits
Low activity, provides good peak shape for acidic and basic
samples
Provides robust column performance for high temperature
bake outs
Individually tested for low bleed, MS certified
(Isothermal/TPGC)*
Stability
Applications

High Boiling Petroleum Products

Simulated Distillation Methods
Long-chained Hydrocarbons
High Molecular Weight Waxes
Polymers/Plastics
Motor Oils
Diesel Fuel
Alternative to Any 100% Dimethylpolysiloxane High Temperature

Phase:
DB-1ht
Rxi-1HT
Petrocol 2887
CP-SimDist
18153
App ID 18153
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
10
Zebron ZB-1HT Inferno

15 meter x 0.32 mm x 0.25 m
7EM-G014-11
Splitless @ 300C, 20 L
50C for 2 min to 320C @ 30C/min for 5 min
FID @ 330C
1. Decane
2. Tetracontane
12
14
min
Zebron ZB-1HT Inferno GC Columns

ID(mm)
df(m)
Temp. LimitsC
5-Meter
0.53
0.10
-60 to 400/430
10-Meter
0.32
0.25
-60 to 400/430
15-Meter
0.25
0.10
-60 to 400/430
0.25
0.25
-60 to 400/430
0.32
0.10
-60 to 400/430
0.32
0.25
-60 to 400/430
0.53
0.15
-60 to 400
20-Meter
0.18
0.18
-60 to 400/430
30-Meter
0.25
0.10
-60 to 400/430
0.25
0.25
-60 to 400/430
0.32
0.10
-60 to 400/430
0.32
0.25
-60 to 400/430
0.53
0.15
-60 to 400
Part No.
Price
7AK-G014-02
7CM-G014-11
7EG-G014-02
7EG-G014-11
7EM-G014-02
7EM-G014-11
7EK-G014-05
7FD-G014-08
7HG-G014-02
7HG-G014-11
7HM-G014-02
7HM-G014-11
7HK-G014-05
Hydrocarbons from Water: DIN EN ISO 9377-2 (DEV H53)
See p. 70
ZB-1HT Test Mix Part No.: AG0-5155

Price:
*0.53 mm ID columns are rated to 400 C max operational

temperature.
Courtesy of Dr. Alexander Ruderisch, Agrolab Labor GmbH, Bruckberg

Phenomenex
73
Zebron GC Columns

ZB-1XT SimDist Metal

New Glass Infusion technology provides higher
efficiency and greater column-to-column reproducibility
Guaranteed high quality - every column is individually
tested
Column Profile
Low
High
Polarity
Bleed
Up to 70% more efficiency than other columns
Temperature Limits
Improved resolution of C50/C52
Stability
Increased accuracy for high temperature simulated

distillation analysis
Applications

Temperature Limits: -60 to 450C

(Isothermal/TPGC)*
Crude Oil
Vacuum Distillates
Petroleum Distillates
Petroleum Fractions
Gasoline Fractions
Simulated Distillations
ASTM Methods D2887, D2887X, D3710,
D6352, D7169
Alternative to:
Agilent J&W DB-HT Sim Dis
Varian CP-SimDist UltiMetal

Restek MXT-1HT SimDist
19264
C7
C8
High Efficiency!
Resolution: 4.81
C44
C50
C52
C9
10
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
20
30
Zebron ZB-1XT SimDist

5 meter x 0.53mm x 0.15m
7AK-G026-05
On-Column @ 33C, 1L
Helium @ 7mL/min (constant flow)
FID @ 450C
C7 to C44 hydrocarbons and POLYWAX 655
Note: Sample in CS2. Chromatogram is baseline subtracted.
40
C100
C90
C80
0
App ID 19264
C60
C70
Hydrocarbons C7C100+: ASTM Method D7169
min
Zebron ZB-1XT SimDist Metal GC Columns

ID(mm)
df(m)
Temp. LimitsC
5-Meter
0.53
0.09
-60 to 450
0.53
0.15
-60 to 450
10-Meter
0.53
0.15
-60 to 450
0.53
0.88
-60 to 450
0.53
2.65
-60 to 400
15-Meter
0.53
0.25
-60 to 450
Part No.
Price
7AK-G026-55
7AK-G026-05
7CK-G026-05
7CK-G026-49
7CK-G026-35
7EK-G026-11
If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7CK-G026-05-B. Some exceptions
may apply. Agilent 6850 and some SRI and process GC systems use only 5 in. cages. See p. 70.
ZB-1XT SimDist Test Mix Part No.: AG0-8645

Price:
*Thicker film (2.65 m df) is rated to 400 C

(Isothermal/TPGC).
74
Phenomenex
Zebron GC Columns

ZB-5
Column Profile
Rugged, versatile low polarity column for general lab

purpose
Low
Resilient to dirty sampleslong column life
Polarity
Low bleed (MS Certified) especially suited to high sensitivity

work using GC/MS
Bleed
Extremely inert for active compounds such as drugs or

pesticides
Stability
High
Temperature Limits
Great column for unknown samples

Applications
Temperature Limits: -60 to 360/370 C

(Isothermal/TPGC)*
Alkaloids
Dioxins
Drugs
Essential Oils/Flavors
FAMEs
Halo-hydrocarbons
PCBs/Aroclors
Pesticides/Herbicides
Phenols
Residual Solvents
Semi-volatiles
Alternative to Any 5%-Phenyl-95%-Dimethylpolysiloxane Phase:

DB-5
HP-5
Rtx-5
HP-PAS-5
MDN-5
Equity-5
SPB-5
OV-5
007-5
AT-5
BPX5
SE-54
BP5
Ultra 2
EC-5
HP-5 Trace
Analysis
CP-SIL 8 CB
Phthalate Esters: EPA Method 606

3
App ID 14876
10
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
20
30
40
min
Zebron ZB-5
30 meter x 0.53 mm x 1.50 m
7HK-G002-28
Split 20:1 @ 300C, 1 L
Helium @ 12.9 mL/ min (constant flow)
FID @ 300C
1. Dimethyl Phthalate
2. Diethyl Phthalate
3. Di-n-butyl Phthalate
4. Butyl Benzyl Phthalate
5. Bis(2-ethylhexyl) Phthalate
6. Di-n-octyl Phthalate
For ultra low bleed, consider using a ZB-5ms, see p. 77

For high temperature analysis, consider using a ZB-5HT,
see p. 78-79
(Isothermal/TPGC).

Phenomenex
Temp. LimitsC
Part No.
-60 to 360/370
-60 to 360/370
-60 to 360/370
-60 to 340/360
-60 to 360/370
-60 to 360/370
-60 to 340/360
-60 to 360/370
-60 to 340/360
-60 to 340/360
7EG-G002-02
7EG-G002-11
7EG-G002-17
7EG-G002-22
7EM-G002-02
7EM-G002-11
7EM-G002-22
7EK-G002-17
7EK-G002-28
7EK-G002-36
-60 to 360/370
7FD-G002-08
-60 to 360/370
-60 to 360/370
-60 to 360/370
-60 to 340/360
-60 to 360/370
-60 to 360/370
-60 to 340/360
-60 to 360/370
-60 to 340/360
-60 to 340/360
-60 to 340/360
7HG-G002-02
7HG-G002-11
7HG-G002-17
7HG-G002-22
7HM-G002-11
7HM-G002-17
7HM-G002-22
7HK-G002-17
7HK-G002-28
7HK-G002-36
7HK-G002-39
-60 to 360/370
-60 to 360/370
-60 to 360/370
-60 to 340/360
-60 to 360/370
-60 to 340/360
-60 to 340/360
7KG-G002-02
7KG-G002-11
7KG-G002-17
7KG-G002-22
7KM-G002-11
7KM-G002-22
7KK-G002-28
Price

ID(mm)
df(m)
15-Meter
0.25
0.10
0.25
0.25
0.25
0.50
0.25
1.00
0.32
0.10
0.32
0.25
0.32
1.00
0.53
0.50
0.53
1.50
0.53
3.00
20-Meter
0.18
0.18
30-Meter
0.25
0.10
0.25
0.25
0.25
0.50
0.25
1.00
0.32
0.25
0.32
0.50
0.32
1.00
0.53
0.50
0.53
1.50
0.53
3.00
0.53
5.00
60-Meter
0.25
0.10
0.25
0.25
0.25
0.50
0.25
1.00
0.32
0.25
0.32
1.00
0.53
1.50
See p. 70.
ZB-5 Test Mix

Part No.: AG0-5155
Price:
75
Zebron GC Columns

ZB-5MSi
Column Profile
Highly inertimproved peak shape for acidic/basic

compounds, drugs of abuse and pesticides
Low
High
Very low bleed (MS certified) levels provide maximum

sensitivity
Polarity
Intense QC specifications ensure column-to-column

performance
Temperature Limits
Bleed
Stability
ESC bonding results in phase stability and high

temperature limits
Applications
Traditional bonding chemistry provides the same

selectivity as the ZB-5 columns
Drugs of abuse
FAMEs
Nitrosamines
Phenols
Pesticides
EPA Methods

(Isothermal/TPGC)
DB-5
HP-5msi
Rtx-5MS
Rtx-5Amine
MDN-5S
HP-5ms
Rxi-5ms
Rxi-5msi
13,14,15
9,10,11
4,5
12
12 16
1718
24
19
20
21
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
22
10
12
14
16
18
26
25
23
20
Zebron ZB-5MSi
30 meter x 0.25 mm x 0.25 m
7HG-G018-11
Split 5:1 @ 240C, 1 L
60C to 140C @ 5C/min to 280C @ 10C/min
MSD @ 230C, 45-450 amu
16. 2,3-Dimethylphenol
1. Phenol
2. 2-Chlorophenol
18. 2,6-Dichlorophenol
3. 2-Methylphenol
19. 4-Chloro-3-methylphenol
4. 4-Methylphenol
20. 2,4,6-Trichlorophenol
5. 3-Methylphenol
22. 2,4-Dinitrophenol
7. 2-Nitrophenol
23. 4-Nitrophenol
8. 2-Ethylphenol
24. 2,3,4,6-Tetrachlorophenol
25. 4,6-Dinitro-2-methylphenol
26. Pentachlorophenol
27. Dinoseb
12. 4-Ethylphenol
13. 3-Ethylphenol
15. Benzoic Acid

22
27
App ID 16410
Phenols
min
Zebron ZB-5MSi GC Columns

ID(mm)
df(m)
10-Meter
0.18
0.18
12-Meter
0.20
0.33
15-Meter
0.25
0.25
30-Meter
0.25
0.25
0.25
0.50
0.25
1.00
0.32
0.25
0.32
0.50
60-Meter
0.25
0.25
Temp. LimitsC
Part No.
Price
-60 to 360/370
7CD-G018-08
-60 to 360/370
7DE-G018-14
-60 to 360/370
7EG-G018-11
-60 to 360/370
-60 to 360/370
-60 to 360/370
-60 to 360/370
-60 to 360/370
7HG-G018-11
7HG-G018-17
7HG-G018-22
7HM-G018-11
7HM-G018-17
-60 to 360/370
7KG-G018-11
See p. 70.
ZB-5MSi Test Mix Part No.: AG0-8362

Price:
For high temperature analysis, consider using a ZB-5HT, see p. 70


76
Phenomenex
Zebron GC Columns

ZB-5ms
A popular and rugged GC column for general use
Column Profile
Arylene Matrix Technology (AMT) provides a highly

stable arylene phase
Low
High
Low bleed (MS Certified) especially suited to high

sensitivity work using GC/MS
Polarity
Fully conditioned within 35 minutes
Temperature Limits
High response for acids and bases = Very low activity
Stability
Enhanced resolution of Polyaromatic Hydrocarbons

(PAHs) and other multi-ring aromatic compounds
Applications
Bleed
Our column of choice for EPA Methods 525, 610, 625,

and 8100
(Isothermal/TPGC)
Acids
Alkaloids
Amines
Dioxins
Drugs
Essential Oils/Flavors
FAMEs
Halo-hydrocarbons
PCBs/Aroclors
Phenols
Residual Solvents
Semi-volatiles
Solvent Impurities
EPA Methods 525, 610,
625, 8100
Alternative to Any MS-Certified 5%-Phenyl-Arylene95%-Dimethylpolysiloxane Phase:

CP-Sil 8 CB MS
DB-5.625
Rtx-5Sil MS
Rxi-5Sil MS
Long Lifetime
Consistent response after more than 700 samples at pH 2!
1.5
Peak Skew
2,4-Dichlorophenol
Hexachlorobutadiene
0.5
140
280
Sample No.
420
560
700
Reproducible Results
1
2
3
5
7
2.0
3.0
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
4.0
5.0
Zebron ZB-5ms
30 meter x 0.25 mm x 0.25 m
7HG-G010-11
Split 1:100 @ 250C, 1.4 L
Hydrogen @ 140C, 40 cm/sec
140C (Isothermal)
FID @ 325C
1. Decane
3. 1,6-Hexanediol
4. 4-Chlorophenol
5. Tridecane
6. 1-Methylnaphthalene
7. 1-Undecanol
8. Tetradecane
10. Pentadecane
Phenomenex
10
6.0
7.0
8.0 min
Lot 4
Lot 3
Lot 2
Lot 1
Zebron ZB-5ms GC Columns

ID(mm)
df(m)
10-Meter
0.10
0.10
0.18
0.18
12-Meter
0.20
0.33
15-Meter
0.25
0.25
20-Meter
0.18
0.18
0.18
0.32
0.18
0.36
25-Meter
0.20
0.33
30-Meter
0.25
0.25
0.25
0.50
0.25
1.00
0.32
0.25
0.32
0.50
0.32
1.00
60-Meter
0.25
0.10
0.25
0.25
0.32
0.25
Temp. LimitsC
Part No.
-60 to 325/350
-60 to 325/350
7CB-G010-02
7CD-G010-08
-60 to 325/350
7DE-G010-14
-60 to 325/350
7EG-G010-11
-60 to 325/350
-60 to 325/350
-60 to 325/350
7FD-G010-08
7FD-G010-51
7FD-G010-53
-60 to 325/350
7GE-G010-14
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
-60 to 325/350
7HG-G010-11
7HG-G010-17
7HG-G010-22
7HM-G010-11
7HM-G010-17
7HM-G010-22
-60 to 325/350
-60 to 325/350
-60 to 325/350
7KG-G010-02
7KG-G010-11
7KM-G010-11
Price
DB-5ms
DB-5ms EVDX
VF-5ms
See p. 70.
ZB-5ms Test Mix Part No.: AG0-7578

Price:


77
Zebron GC Columns
ZB-5HT Inferno
Column Profile
First non-metal columns stable to 430C
Low
Robust column for high temperature bake outs analysis,

such as biodiesel, long-chain hydrocarbons, polymers,
and high molecular weight compounds
High
Polarity
Bleed
Provides true boiling point separation for hydrocarbon

distillation methods
Temperature Limits
Stability

Applications
Low activity, provides good peak shape for acidic and

basic samples

(Isothermal/TPGC)*
High Boiling Petroleum Products

Triglycerides
Simulated Distillation Methods
Diesel Fuel
Long-chained Hydrocarbons
Motor Oils
Polymers/Plastics
Surfactants
High Molecular Weight Waxes
Alternative to Any 5%-Phenyl-95% Dimethylpolysiloxane High

Temperature Phase:
DB-5ht
HT-5
XTI-5HT
Lower Bleed!*

Agilent J&W DB-5ht
Varian VF-5ht
23
21
19
7.5
17
15
13
11
9
Comparison of retention of Pentadecane between the Zebron ZB-5HT Inferno column versus a
traditional 5%-Phenyl - 95%-dimethylpolysiloxane column. Note that the VF-5ht column died
around 40 hours at 400C whereas the Zebron ZB-5HT Inferno column maintained great retention
of Pentadecane over 100 hours.
6.5
7
5
10
15
20
25
30
35 min
6
0
20
40
60
80
100
120
Hours at 400 C
Conditions for all columns**:

Dimensions: 30 meter x 0.25 mm x 0.10 m
Injection: 1.0 L of test mix AG0-7578
Carrier Gas: Helium @ 1.9 mL/min (constant flow)
Oven Program: 120C Isothermal
Detector: Flame Ionization Detector (FID) @ 400C
Sample: Pentadecane
How does the lifetime test work?

Hydrocarbons are a good way to measure the stability and lifetime
of a non-polar column. Because their interaction with the phase is
mostly based on London Dispersion forces, any change in retention
time is correlated with phase loss. This will result in increased bleed
and poor reproducibility.
In the lifetime study above, the Zebron ZB-5HT Inferno column has
twice the lifetime as the two comparative columns. For the test,
all columns were held at 400C for 2 hours and then the oven
was lowered to 120C for Pentadecane analysis. The Varian HT
column broke just after 40 hours at 400C. The ZB-5HT had the
same retention for Pentadecane at 100 hours as the Agilent J&W
DB-5ht column at 40 hoursover 2X the lifetime!
78

Agilent J&W DB-5ht
25
pA
Retention of Pentadecane at 120 C (min)
Stx-5HT
Zebron Inferno Columns Win In The Lifetime Test
8.5
VF-5ht
Conditions for all columns**:

Injection: Null Injection @ 250C
Carrier Gas: Hydrogen @ 11 psi (Constant Pressure)
Oven Program: 120C for 3 min to 320C @ 30C/min (hold 5 min) to
340C @ 30C/min (hold 5 min) to 360C @ 30C/min
(hold 5 min) to 380C @ 30C/min (hold 5 min) to 400C
@ 30C/min (hold 5 min)
*The shaded area depicts the bleed criteria for MS certified columns on a MS detector. MS bleed
certification values are typically read at 320 C. This demonstrates the low bleed capabilities of the
ZB-5HT. It meets MS certification limits even at 360 C!
**All columns used for above tests were new/never used, prior to this testing and purchased either directly
from the original manufacturer or through an authorized distributor. All testing was carefully controlled to
ensure conditions were similar for all columns involved. The comparative data may not be representative
of every application.
Agilent and J&W are registered trademarks of Agilent Technologies, Inc. Varian is a registered trademark
under license to Varian, Inc.
Phenomenex
Zebron GC Columns

ZB-5HT Inferno (contd)

16054
Butter Triglycerides
Paraffin Wax
4th injection
136th injection
10
12
16
18
20
22
min

15meter x 0.32mm x 0.10m
7EM-G015-02
Direct on column @ 43C, 0.1L
Helium @ 1.9mL/min (35cm/sec) (constant flow)
40C for 2min to 430C @ 20C for 10min
FID @ 430C
Paraffin Wax
16077
Mineral Oil Using H-53 Conditions

2
App ID 16077
Column:
Dimensions:
Part No.:
Injection:
Oven Program:
Carrier Gas:
Detector:
Sample:
10
15
10
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
20

30 meter x 0.25 mm x 0.10 m
7HG-G015-02
On-Column @ 53C, 0.1 L
FID @ 415C
Fuel was 10 mg/mL in dichloromethane with 50 ppm markers
1. Decane (C10)
min
20
30 min

15 meter x 0.32mm x 0.10m
7EM-G015-02
On-Column @ 103C, 2L
Helium @ 1.8mL/min (constant flow)
FID @ 400C
Butter
Zebron ZB-5HT Inferno GC Columns
ID(mm)
df(m)
Temp. LimitsC
Part No.
10-Meter with 2-Meter Spliced Guard (0.53 mm ID)
0.32
0.10
-60 to 400/430
7CM-G015-02-GST
15-Meter
0.25
0.10
-60 to 400/430
7EG-G015-02
0.25
0.25
-60 to 400/430
7EG-G015-11
0.32
0.10
-60 to 400/430
7EM-G015-02
0.32
0.25
-60 to 400/430
7EM-G015-11
0.53
0.15
-60 to 400
7EK-G015-05
15-Meter with 2-Meter Spliced Guard (0.53 mm ID)
0.32
0.10
-60 to 400/430
7EM-G015-02-GST
20-Meter
0.18
0.18
-60 to 400/430
7FD-G015-08
30-Meter
0.25
0.10
-60 to 400/430
7HG-G015-02
0.25
0.25
-60 to 400/430
7HG-G015-11
0.32
0.10
-60 to 400/430
7HM-G015-02
0.32
0.25
-60 to 400/430
7HM-G015-11
0.53
0.15
-60 to 400
7HK-G015-05
60-Meter
0.25
0.25
-60 to 400/430
7KG-G015-11
Price
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
14
App ID 16054
App ID 16089
Only 1.5% retention difference after

23 hours in extreme conditions
See p. 70.

Price:
*0.53 mm ID columns are rated to 400 C max operational
temperature.

Phenomenex
79
Zebron GC Columns

ZB-XLB
Unique, low polarity si-arylene column
Column Profile
Engineered specifically for use with bleed sensitive

detectors such as MS
Low
High
Polarity
Provides alternate selectivity to standard 5-type phases

Often used for confirmation of pesticides, PCBs, or
other environmental samples
Bleed
Temperature Limits
Stability
Good tool for sample screening to identify unknown

contaminants
Applications
Temperature Limits: 30 to 340/360 C

(Isothermal/TPGC)*
Polychlorinated Biphenyls (PCBs)

Pesticides
Herbicides
Similar* to:
MDN-12
DB-XLB
Rtx-XLB
*not exact equivalent, selectivity might be different
Chlorinated Pesticides: EPA Method 8081A
9
11
10 12
14
15 17 18
16
20
13
App ID 16414
8
6
19
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
10
11
12
13
14
15
Zebron ZB-XLB
30 meter x 0.25 mm x 0.25 m
7HG-G019-11
Split 111:1 @ 250 C, 1.5 L
110 C to 320 C at 15 C/min and hold until last peak elutes
ECD @ 350C
1. a-BHC
2. g-BHC
3. b-BHC
4. d-BHC
5. Heptachlor
6. Aldrin
7. Heptachlor epoxide
8. g-Chlordane
9. a-Chlordane
10. Endosulfan I
11. 4,4-DDE
12. Dieldrin
13. Endrin
14. 4,4-DDD
15. Endosulfan II
16. Endrin aldehyde
17. 4,4-DDT
18. Endosulfan sulfate
19. Methoxychlor
20. Endrin ketone
16 min
Zebron ZB-XLB GC Columns
ID(mm)
df(m)
10-Meter
0.18
0.18
15-Meter
0.25
0.25
20-Meter
0.18
0.18
30-Meter
0.25
0.25
0.25
0.50
0.32
0.25
0.32
0.50
0.53
1.50
60-Meter
0.25
0.25
Temp. LimitsC
Part No.
30 to 340/360
7CD-G019-08
30 to 340/360
7EG-G019-11
30 to 340/360
7FD-G019-08
30 to 340/360
30 to 340/360
30 to 340/360
30 to 340/360
30 to 320/340
7HG-G019-11
7HG-G019-17
7HM-G019-11
7HM-G019-17
7HK-G019-28
30 to 340/360
7KG-G019-11
Price
See p. 70.
ZB-XLB Test Mix Part No.: AG0-7578

Price:

(Isothermal/TPGC).

80
Phenomenex
Zebron GC Columns

ZB-XLB-HT Inferno
Rugged, non-metal si-arylene GC column stable to 400 C
Column Profile
Robust column for high temperature bake outs and analysis,

such as high molecular weight compounds
Low
Provides unique selectivity for conformational analyses
High
Polarity
Bleed
Longer lifetime with high temperature, polyimide coated,

fused silica tubing
Temperature Limits
samples
Stability
Good tool for general screening to identify unknown samples
Applications
Polychlorinated Biphenyls (PCBs)

Pesticides
Herbicides
Temperature Limits: 30 to 400C

(Isothermal/TPGC)
EPA Methods
Similar* to:
MDN-12
DB-XLB
Rtx-XLB
Aroclor
18177 1242: DIN Method 51527
1
Congeners 28 and 31
Separated
21
4
App ID 18177
5
8
11
6 7
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
10
12
10
9
14
12
13 14
16
15 16 17
18
1819 20
20
Zebron ZB-XLB-HT
30 meter x 0.25 mm x 0.25 m
7HG-G024-11
Split 2:1 @ 250C 1 L
50C for 0.5 min to 210C @ 40C/min for 3 min to 230C @
30C/min for 5 min to 250C @ 30C/min for 5 min to 320C @
40C/min for 2 min
Detector: ECD @ 350C
Comment: Pressure pulse used at 40 psi for first 0.25 min
Sample: Total concentration of aroclors was 90 ppm in isooctane
1. TCMX
12. BZ# 138
2. BZ# 31
13. BZ# 126
3. BZ# 28
14. BZ# 167
4. BZ# 52
15. BZ# 156
5. BZ# 101
16. BZ# 180
6. BZ# 77
17. BZ# 157
7. BZ# 123
18. BZ# 170
8. BZ# 118
19. BZ# 169
9. BZ# 153
20. BZ# 189
10. BZ# 114
21. DCB
11. BZ# 105
Phenomenex
Zebron ZB-XLB-HT Inferno GC Columns

ID(mm)
df(m)
Temp. LimitsC
15-Meter
0.25
0.10
30 to 400
0.25
0.25
30 to 400
0.32
0.10
30 to 400
20-Meter
0.18
0.18
30 to 400
30-Meter
0.25
0.10
30 to 400
0.25
0.25
30 to 400
0.32
0.25
30 to 400
60-Meter
0.25
0.25
30 to 400
Part No.
Price
7EG-G024-02
7EG-G024-11
7EM-G024-02
7FD-G024-08
7HG-G024-02
7HG-G024-11
7HM-G024-11
7KG-G024-11
See p. 70.
22min
ZB-XLB-HT Test Mix

Part No.: AG0-7578
Price:


81
Zebron GC Columns

ZB-35
Intermediate polarity column with temperature limits up to
360 C allows high molecular weight analysis
Excellent inertness to minimize analyte adsorption, improve
efficiency, and reproducibility
More rugged (longer column life) than other polar phases
18
Polarity
Bleed
Stability

007-11
Sup-Herb
BPX608
High
Applications

(Isothermal/TPGC)
OV-11
HP-35ms
SPB-608 HP-35
Rtx-35MS MDN-35
Low
Temperature Limits
Excellent for trace analysis with bleed-sensitive detectors

(MS, FID, ECD, NPD)
DB-35ms
SPB-35
AT-35
Column Profile
DB-35
EC-35
Aroclors
Semi-volatiles
Amines
EPA Methods 508, 608, 8081, 8141, 8151
Pesticides
Drugs of Abuse
Pharmaceuticals
Steroids
Rtx-35
BPX35
Common Drug Screen

25
22
28
27
16
11
13
9
2
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
6 7 8
10
15
14
17
18
19 21
20
23
29
12
26
24
10
30
12
App ID 16010
min
Zebron ZB-35
30 meter x 0.25 mm x 0.25 m
7HG-G003-11
Split 10:1 @ 225C, 1.5 L
120 C for to 180 C @ 25 C/min to 200 C/min @
6 C/min to 300 C/min @ 20 C/min for 3 min
Sample: All analytes are 25 ppm except nicotine at 100 ppm
1. Nicotine
11. Benzphetamine
21. Bromopheniramine
2. Ibuprofen
12. Meprobamate
22. Chlorcyclizine
3. Allobarbital
13. Dimenhydrinate
23. Cocaine
4. Acetaminophen
14. Hexobarbital
24. Benactyzine
5. Aprobarbital
15. Doxylamine
25. Codeine
6. Butalbital
16. Caffeine
26. Diazepam
7. Amobarbital
17. Chlorpheniramine 27. Morphine
8. Pentobarbital
18. Methapyrilene
28. Hydrocodone
9. Phenacetin
19. Phenobarbital
29. Oxymorphone
10. Secobarbital
20. Procaine
30. Heroin

ID(mm)
df(m)
10-Meter
0.10
0.10
15-Meter
0.25
0.25
0.25
0.50
0.32
0.25
0.53
1.00
30-Meter
0.25
0.25
0.25
0.50
0.32
0.25
0.32
0.50
0.53
0.50
0.53
1.00
60-Meter
0.25
0.25
0.32
0.25
Temp. LimitsC
Part No.
Price
40 to 340/360
7CB-G003-02
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
7EG-G003-11
7EG-G003-17
7EM-G003-11
7EK-G003-22
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
40 to 340/360
7HG-G003-11
7HG-G003-17
7HM-G003-11
7HM-G003-17
7HK-G003-17
7HK-G003-22
40 to 340/360
40 to 340/360
7KG-G003-11
7KM-G003-11
See p. 70.
ZB-35 Test Mix

Part No.: AG0-5156
Price:


82
Phenomenex
Zebron GC Columns

ZB-35HT Inferno
First non-metal, 35%-Phenyl columns stable to 400 C
Column Profile

Low
High
18
Polarity
Robust column for high temperature analysis
Bleed
Great for high molecular weight compounds
Temperature Limits
Eliminate carry-over with high temperature bake outs
Stability
samples
Applications

Temperature Limits: 40 to 400 C
(Isothermal/TPGC)
Aroclors
Semi-volatiles
Amines
EPA Methods 508, 608, 8081, 8141, 8151
Pesticides
Drugs of Abuse
Pharmaceuticals
Steroids
Chemicals
Similar to Any 35%-Phenyl-65%-Dimethylpolysiloxane High

Temperature Phase:
DB-35ms
SPB-35
AT-35
OV-11
HP-35ms
SPB-608 HP-35
Rtx-35MS MDN-35
007-11
Sup-Herb
BPX608
DB-35
EC-35
Rtx-35
BPX35
Conditions for all columns:

Injection: Split 20:1 @ 200C, 1 L
Carrier Gas Helium @ 1.7 mL/min (constant flow)
Oven Program: 100C to 320C @ 30C/min for 5 min to 340C @
20C/min for 5 min to 360C @ 20C/min for 5 min to
380C @ 20C/min for 5 min to 400C @ 20C/min for 5
min to 100C @ 30C/min for 8 min
Zebron ZB-35HT
pA
Agilent J&W DB-35ms
60
Varian VF-35ms
Restek Rtx-35MS
40
20
2.5
7.5
10
12.5
15
17.5
20
22.5
min
Zebron ZB-35HT GC Columns

ID(mm)
df(m)
15-Meter
0.25
0.10
0.25
0.25
0.32
0.10
0.32
0.25
20-Meter
0.18
0.18
30-Meter
0.25
0.10
0.25
0.25
0.32
0.25
Temp. LimitsC
Part No.
40 to 400
40 to 400
40 to 400
40 to 400
7EG-G025-02
7EG-G025-11
7EM-G025-02
7EM-G025-11
40 to 400
7FD-G025-08
40 to 400
40 to 400
40 to 400
7HG-G025-02
7HG-G025-11
7HM-G025-11
Price
See p. 70.
Lower Bleed!

Price:


Phenomenex
83
Zebron GC Columns

ZB-50
Column Profile
High polarity column with temperature limits up to

340 C allows high temperature bake out to remove
contaminants
Low
High
24
Polarity
Excellent inertness to minimize analyte adsorption,

improve efficiency, and reproducibility
Bleed
More rugged (longer column life) than other polar phases
Temperature Limits
Excellent for trace analysis with bleed-sensitive detectors
Stability
Great for drug screening and environmental compounds

Applications


(Isothermal/TPGC)
Antidepressants
Aroclors
Drugs of Abuse
Cholesterols
Glycols
Triglycerides
Steroids
EPA Methods 508, 608, 8081, 8141, 8151

DB-17
Rtx-50
SPB-50
CP-Sil 24 CB
AT-50
DB-17ms
SPB-17
HP-50+
DB-17ht
SP-2250
BPX50
007-17
DB-17 EVDX
6
7
4 5
3
22
16 18
12, 13
15
11
17
23
24
19 20
25
21
10
27
14
28
29
26
4
10
12
14
16
18
20
22
16
24
26
28
30
min
22
18
15
17
23
19
20
21
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
20
22
Zebron ZB-50
30 meter x 0.25 mm x 0.25 m
7HG-G004-11
Pulsed Splitless for 0.5 min @ 320 C, 1 L
80 C to 265 C @ 15 C/min to 290 C @ 5 C/min to
330 C at 20 C/min for 15 min
Detector: MSD @ 330 C; 45-450 amu
16. Benzo[k]fluoranthene
17. Benzo[j]fluoranthene
3. Acenaphthalene
18. Benzo[a]pyrene
4. Acenaphthene
19. 3-Methylcholanthrene
5. Fluorene
20. Dibenz[a,h]acridine
6. Phenanthrene
21. Dibenz[a,j]acridine
7. Anthracene
8. Fluoranthene
9. Pyrene
10. Benzo[c]fluorene
25. 7H-Dibenzo[c,g]carbazole
26. Dibenzo[a,l]pyrene
12. Chrysene
27. Dibenzo[a,e]pyrene
13. Cyclopenta[c,d]pyrene
28. Dibenzo[a,i]pyrene
14. 5-Methylchrysene
29. Dibenzo[a,h]pyrene
84
App ID 16090
PAHs including European Analytes

ID(mm)
df(m)
10-Meter
0.10
0.10
0.53
2.00
15-Meter
0.25
0.15
0.25
0.25
0.32
0.25
0.32
0.50
0.53
1.00
30-Meter
0.25
0.25
0.25
0.50
0.32
0.25
0.32
0.50
0.53
1.00
60-Meter
0.25
0.25
0.25
0.50
Temp. LimitsC
Part No.
Price
40 to 320/340
40 to 320/340
7CB-G004-02
7CK-G004-32
40 to 320/340
40 to 320/340
40 to 320/340
40 to 320/340
40 to 320/340
7EG-G004-05
7EG-G004-11
7EM-G004-11
7EM-G004-17
7EK-G004-22
40 to 320/340
40 to 320/340
40 to 320/340
40 to 320/340
40 to 320/340
7HG-G004-11
7HG-G004-17
7HM-G004-11
7HM-G004-17
7HK-G004-22
40 to 320/340
40 to 320/340
7KG-G004-11
7KG-G004-17
See p. 70.
ZB-50 Test Mix

Part No.: AG0-5157
Price:


Phenomenex
Zebron GC Columns

ZB-624
Widely used phase to separate volatile organic flavor and
fragrance additives and residual solvents in industrial or
pharmaceutical products (OVIs)
Popular choice for residual solvent testing
Column Profile
Low
High
13
Polarity
Bleed
Excellent for US EPA Methods 501.3, 502.2, 503.1, 524.2, 601,

602, 624, 8010, 8015, 8020, 8021, 8240, 8260
Specifically designed for the separation of volatile organic
compounds (VOCs)
Temperature Limits
Stability
Applications
Increased temperature limit speeds run times and

re-equilibration
Volatile Organic Compounds (VOCs)

Residual Solvents
EPA Methods 501.3, 502.2, 503.1, 524.2, 601, 602, 624, 8010, 8015, 8020, 8021,
8240, 8260
Pharmaceuticals

Temperature Limits: -20 to 260C
Alternative to Any 6%-Cyanopropylphenyl94%-Dimethylpolysiloxane Phase:

DB-624
AT-624
DB-VRX
Rtx-1301
CP-1301
Rtx-624
BP624
007-502
HP-VOC
007-624
DB-1301
SPB-1301
CP-Select 624 CB
SPB-624
Rtx-VMS
4
2
App ID 16348
3
4
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
10
Zebron ZB-624
30 meter x 0.32 mm x 1.80 m
7HM-G005-31
Split 5:1 @ 140C, 1 mL
Helium @ 35 cm/sec (constant flow)
40 C for 20 min to 240 C @ 10 C/min for 20 min
FID @ 250C
1. 1,1-Dichloroethene
2. 1,1,1-Trichloroethane
3. Carbon tetrachloride
4. Benzene
5. 1,2-Dichloroethane
12
min

ID(mm)
df(m)
20-Meter
0.18
1.00
30-Meter
0.25
1.40
0.32
1.80
0.53
3.00
60-Meter
0.25
1.40
0.32
1.80
0.53
3.00
75-Meter
0.53
3.00
105-Meter
0.53
3.00
Temp. LimitsC
Part No.
-20 to 260
7FD-G005-22
-20 to 260
-20 to 260
-20 to 260
7HG-G005-27
7HM-G005-31
7HK-G005-36
-20 to 260
-20 to 260
-20 to 260
7KG-G005-27
7KM-G005-31
7KK-G005-36
-20 to 260
7LK-G005-36
-20 to 260
7NK-G005-36
Price
USP <467>: Residual Solvents

Procedure A - Class 1
See p. 70.
ZB-624 Test Mix Part No.: AG0-5159

Price:

For more applications on residual solvents,

see pp. 128-129
Phenomenex

85
Zebron GC Columns

ZB-1701
Fast run and re-equilibration times for enhanced sample
throughput and productivity
Provides alternate selectivity to phenyl phases with
similar polarity
High
19
Bleed
Temperature Limits

(Isothermal/TPGC)*
Stability
Alternative to Any 14%-Cyanopropylphenyl-86%Dimethylpolysiloxane Phase:

007-1701
BP10
AT-1701
Low
Polarity
DB-1701
SPB-1701
Rtx-VMS
Column Profile
Equity 1701
Rtx-1701
OV-1701
Applications

CP-Sil 19 CB
Aromatic Amines
Pharmaceutical intermediates
PCBs
Alcohols
Aromatic Hydrocarbons
Phenols
PAHs
Tranquilizers
Steroids
Esters
TMS Sugars
Drugs
Amines
Solvents
14
13
12
43
78
10
6
App ID 14789
9
2
11
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-1701
30 meter x 0.25 mm x 0.25 m
7HG-G006-11
Split 15:1 @ 220C, 1 L
60C for 1 min to 110C @ 30C/min to 135C @ 9C/min
to 260C @ 30C/min for 2 min
Detector: MSD @ 180C
Sample: Analytes are at 1.58 mg/mL each
1. Piperidine
2. 2-Methylpiperidine
3. Aniline
4. Benzylamine
5. -Phenylethylamine
6. N-Methylaniline
7. m-Toluidine
8. o-Toluidine
9. N,N-Dimethylaniline
11. N-Ethylaniline
12. 2,4-Dimethylaniline
13. N,N-Diethylaniline
14. Dibenzylamine
min

ID(mm)
df(m)
15-Meter
0.25
0.25
0.32
0.25
0.32
1.00
30-Meter
0.25
0.25
0.25
1.00
0.32
0.25
0.32
1.00
0.53
1.00
60-Meter
0.25
0.25
0.32
0.25
Temp. LimitsC
Part No.
-20 to 280/300
-20 to 280/300
-20 to 260/280
7EG-G006-11
7EM-G006-11
7EM-G006-22
-20 to 280/300
-20 to 260/280
-20 to 280/300
-20 to 260/280
-20 to 260/280
7HG-G006-11
7HG-G006-22
7HM-G006-11
7HM-G006-22
7HK-G006-22
-20 to 280/300
-20 to 280/300
7KG-G006-11
7KM-G006-11
Price
See p. 70.

Price:
For enhanced response to Endrin and DDT,

consider using ZB-1701P, see p. 87
(Isothermal/TPGC).

86
Phenomenex
Zebron GC Columns

ZB-1701P
Column Profile
Specially tested to ensure response of DDT, Endrin,

Endrin Aldehyde, and Endrin Ketone
Low
Fast run and re-equilibration times for enhanced sample

throughput and productivity
Guaranteed column for pesticide analysis
High
19
Polarity
Bleed
Temperature Limits
Stability

(Isothermal/TPGC)*
Applications
Nitrogen Containing Pesticides
Alternative to:
DB-1701P
Organophosphorous Pesticides
Aroclors
Chlorinated Pesticides: EPA Method 8081

3
4 5 6
18
19
10 12131415
16 17
11
22
23
21
20
10
12
14
24
min
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-1701P
30 meter x 0.25 mm x 0.25 m
7HG-G012-11
Splitless @ 240C, 1 L
100C to 200C @ 25C/min to 240C @ 6C/min to
265C @ 20C/min for 5 min
Sample: All compounds are 20 ppm
1. 1-Bromo-2-Nitrobenzene (IS)
2. Tetrachloro-m-xylene (Surr)
3. -BHC
4. Pentachloronitrobenzene (IS)
5. -BHC (Lindane)
6. Heptachlor
7. Aldrin
8. -BHC
9. -BHC
10. Heptachlor Epoxide
11. Endosulfan I
12. -Chlordane
13. -Chlordane
14. DDE
15. Dieldrin
16. Endrin
17. DDD
18. Endosulfan II
19. DDT
20. Endrin Aldehyde
21. Methoxychlor
22. Endosulfan Sulfate
23. Endrin Ketone
24. Decachlorobiphenyl (Surr)
Zebron ZB-1701P GC Columns
ID(mm)
df(m)
30-Meter
0.25
0.25
0.32
0.25
0.53
1.00
Temp. LimitsC
Part No.
-20 to 280/300
-20 to 280/300
-20 to 260/280
7HG-G012-11
7HM-G012-11
7HK-G012-22
Price
App ID 15881
See p. 70.

Price:

(Isothermal/TPGC).

Phenomenex
87
Zebron GC Columns

ZB-WAX
Column Profile
High polarity column with low bleed (MS certified) for

improved results
Low
High
Highly stable, long lifetime
Polarity
Low activity for amines
Bleed
Bonded, solvent rinsible
Temperature Limits
Excellent chromatography of polar complex mixtures
Stability
57
Widely used for profiling and fingerprinting

Close equivalent to USP Phase G16
Applications
Temperature Limits: 40 to 250/260C

(Isothermal/TPGC)
Alternative to Any Polyethylene Glycol Phase:

HP-INNOWax
Rtx-WAX
CP-Wax 57 CB
007-CW
DB-WAXetr
Met-Wax
Omegawax
EC-Wax
Stabilwax-DB
SOLGEL-WAX
FAMEWAX
4
6
9
7 8
11
10
1.5
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
2.5
3.5
4.5
5.5
Zebron ZB-WAX
30 meter x 0.32 mm x 0.50 m
7HM-G007-17
Split 20:1 @ 250C, 0.2 L
Helium @ 2 mL/min (constant flow)
60C to 75C @ 15C/min to 90C @ 3C/min (hold 3 min)
FID @ 300C
1. Pentane
2. Heptane
3. Solvent (methylene chloride)
4. Benzene
5. Decane
6. Toluene
7. Ethylbenzene
8. p-Xylene
9. m-Xylene
10. Dodecane
11. o-Xylene
App ID 14895
Flavors / Fragrances
Glycols
Pharmaceuticals
Solvents
Styrene
Xylenes
Basic Compounds
BTEX in Under 6 Minutes!

2
Alcohols
Aldehydes
Aromatics
Essential oils
6.5 min
Zebron ZB-WAX GC Columns

ID(mm)
df(m)
10-Meter
0.10
0.10
15-Meter
0.25
0.25
0.32
0.25
0.32
0.50
0.53
1.00
20-Meter
0.18
0.18
30-Meter
0.25
0.15
0.25
0.25
0.25
0.50
0.25
1.00
0.32
0.15
0.32
0.25
0.32
0.50
0.53
0.50
0.53
1.00
60-Meter
0.25
0.15
0.25
0.25
0.25
0.50
0.32
0.25
0.32
0.50
0.32
1.00
0.53
1.00
Temp. LimitsC
Part No.
40 to 250/260
7CB-G007-02
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
7EG-G007-11
7EM-G007-11
7EM-G007-17
7EK-G007-22
40 to 250/260
7FD-G007-08
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
7HG-G007-05
7HG-G007-11
7HG-G007-17
7HG-G007-22
7HM-G007-05
7HM-G007-11
7HM-G007-17
7HK-G007-17
7HK-G007-22
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
7KG-G007-05
7KG-G007-11
7KG-G007-17
7KM-G007-11
7KM-G007-17
7KM-G007-22
7KK-G007-22
Price
See p. 70.
ZB-WAX Test Mix Part No.: AG0-5158

Price:


88
Phenomenex
Zebron GC Columns

ZB-WAXplus
100% aqueous stable, excellent for aqueous samples
Column Profile
Extremely inert for acidic compounds
Low
High
52
Enhanced selectivity for low boiling solvents
Polarity
High retention of alcohols and chlorinated solvents
Bleed
Increased efficiency at 20C
Temperature Limits
Bonded and solvent rinsible
Stability

(Isothermal/TPGC)*
Applications
Alternative to Any Polyethylene Glycol Phase:

DB-WAX
PEG 20M
HP-20M
Stabilwax
Supelcowax-10
AT-WAX
CP-WAX 52 CB
AT-AquaWax
Carbowax 20M
Permabond CW 20M
CAM
BP20
Enhanced Aqueous Stability
7,8
Scottish Single Malt Whiskey

60% Aqueous!
50
40
23
30
App ID 15817
pA
90
60
20
10
0
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
10
Acids (free)
Essential oils
Flavors / Fragrances
Styrene
Glycols
Pharmaceuticals
Solvents
Xylene Isomers
12
Zebron ZB-WAXPLUS
30 meter x 0.25 mm x 0.25 m
7HG-G013-11
Split 30:1 @ 140C, 0.2 L
35 C for 5 min to 85 C @ 10 C/min to 200 C @
25 C/min for 1 min
Sample: 1. Acetaldehyde
2. Ethyl Acetate
3. Methanol
4. Ethanol
5. Propanol
6. Isobutanol
7. 2-Methylbutanol
8. 3-Methylbutanol
14
m in
Zebron ZB-WAXPLUS GC Columns

ID(mm)
df(m)
Temp. LimitsC
10-Meter
0.10
0.10
20 to 250/260
15-Meter
0.25
0.25
20 to 250/260
0.53
1.00
20 to 230/240
20-Meter
0.18
0.18
20 to 250/260
30-Meter
0.25
0.25
20 to 250/260
0.25
0.50
20 to 250/260
0.32
0.25
20 to 250/260
0.32
0.50
20 to 250/260
0.32
1.00
20 to 230/240
0.53
1.00
20 to 230/240
60-Meter
0.25
0.15
20 to 250/260
0.25
0.50
20 to 250/260
0.25
0.25
20 to 250/260
0.32
0.25
20 to 250/260
0.32
0.50
20 to 250/260
0.53
1.00
20 to 230/240
Part No.
Price
7CB-G013-02
7EG-G013-11
7EK-G013-22
7FD-G013-08
7HG-G013-11
7HG-G013-17
7HM-G013-11
7HM-G013-17
7HM-G013-22
7HK-G013-22
7KG-G013-05
7KG-G013-17
7KG-G013-11
7KM-G013-11
7KM-G013-17
7KK-G013-22
Water Reproducibility of ZB-WAXPLUS
70
Alcoholic Beverages
Alcohols
OVIs
Aldehydes
Aromatics
Wax columns provide optimal selectivity for many aqueous

soluble compounds such as those found in alcoholic beverages
or glycol samples. Historically, Polyethylene Glycol (PEG) phases
have been unstable with aqueous samples resulting in poor reproducibility and decreased lifetime. The ZB-WAXplus bonding
procedure results in exceptional stability to repeated injections
of aqueous matrices.
80
See p. 70.
ZB-WAXPLUS Test Mix

Part No.: AG0-7869
Price:

(Isothermal/TPGC).

Phenomenex
89
Zebron GC Columns

ZB-FFAP
High polarity column excellent thermal and chemical stability
Column Profile
Low
Provides better peak shape for underivatized acids
High
58
Especially suited for organic acids, free fatty acids,

and alcohols
Polarity
Bonded, solvent rinsable FFAP Phase
Temperature Limits
Replaces OV-351
Stability
Bleed
Close equivalent to USP Phase G35
Applications

(Isothermal/TPGC)
Alternative to Any Nitroterephthalic Acid Modified

Polyethylene Glycol:
DB-FFAP
BP21
CP-WAX 58 CB
Stabilwax-DA
AT-1000
SPB-1000
EC-1000
HP-FFAP
Nukol
Acrylates
Alcohols
Aldehydes
Free Fatty Acids
Ketones
Volatile Free Acids
Organic Acids
Phenols
007-FFAP
PE-FFAP
CP-FFAP-CB
8
5
1
3
2
4
12
13
10
6
11
14 15
10
15
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
20
25
App ID 14858
Unsaturated Free Fatty Acids
30
Zebron ZB-FFAP
60 meter x 0.25 mm x 0.25 m
7KG-G009-11
Split 40:1 @ 220C, 0.2 L
FID @ 250C
1. Myristic Acid (C14:0)
2. Myristoleic Acid (C14:1c)
3. Palmitic Acid (C16:0)
4. Palmitelaidic Acid (C16:1t)
5. Palmitoleic Acid (C16:1c)
6. Stearic Acid (C18:0)
7. Elaidic Acid (C18:1t)
8. Oleic Acid (C18:1c)
9. Linolelaidic Acid (C18:2t)
10. Linoleic Acid (C18:2c)
11. Linolenic Acid (C18:3c)
12. Arachidic Acid (C20:0)
13. Gondonic Acid (C20:1c)
14. Behenic Acid (C22:0)
15. Erucic Acid (C22:1c)
35
40
min
Zebron ZB-FFAP GC Columns

ID(mm)
df(m)
15-Meter
0.25
0.25
0.32
0.25
0.32
0.50
0.53
1.00
30-Meter
0.25
0.25
0.32
0.25
0.32
0.50
0.32
1.00
0.53
1.00
50-Meter
0.32
0.50
60-Meter
0.25
0.25
Temp. LimitsC
Part No.
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
7EG-G009-11
7EM-G009-11
7EM-G009-17
7EK-G009-22
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
40 to 250/260
7HG-G009-11
7HM-G009-11
7HM-G009-17
7HM-G009-22
7HK-G009-22
40 to 250/260
7JM-G009-17
40 to 250/260
7KG-G009-11
Price
See p. 70.
ZB-FFAP Test Mix Part No.: AG0-5158

Price:


90
Phenomenex
Zebron GC Columns

ZB-Bioethanol
Specially designed for fast and accurate bioethanol
analysis
Provides accurate and reproducible results for Certificate
of Analysis (COA)
Resolve methanol and ethanol from all other denaturant
peaks
Great resolution of fusel alcohols
Allows for quick bake out in between runs to eliminate
contaminates
(Isothermal/TPGC)
Applications
Bioethanol
Alcohol
Fusel Alcohols
Determination of Denatured Bioethanol: ASTM Method D 5501

3
16668
10
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Instrument:
Sample:
15 min
Zebron ZB-1
100 meter x 0.25 mm x 0.50 m
7MG-G001-17
Split 50:1 @ 300C, 1 L
FID @ 300C
Shimadzu GC-2010 with Flame Ionization
1. Methanol
2. Ethanol
3. Denaturant
App ID 16668
App ID 16663
5 min Analysis!
202 min
min
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Instrument:
Zebron ZB-Bioethanol
15 meter x 0.25 mm x 1.00 m
7EG-G020-22
Split 50:1 @ 300C, 1 L
Hydrogen @ 25 cm/sec (constant flow)
55C for 1.7 min to 260C @ 40C/min (hold 2.67 min)
FID @ 300C
Shimadzu GC-2010 with Flame Ionization Detection and
AOC-20i Automatic Liquid
Sample: 1. Methanol
2. Ethanol
3. Denaturant
For Bioethanol fermentation monitoring,

use Rezex-ROA HPLC columns, see p. 229

Phenomenex
Zebron ZB-Bioethanol GC Columns

ID(mm)
df(m)
Temp. LimitsC
15-Meter
0.25
1.00
-60 to 340/360
30-Meter
0.25
1.00
-60 to 340/360
Part No.
Price
7EG-G020-22
7HG-G020-22
See p. 70.
91
Zebron GC Columns
ZB-MultiResidue 1 and 2
~exclusive~
Proprietary phases specially designed for the separation

of all types of pesticides, herbicides, and insecticides
Provides baseline resolution and confirmation of all 20
chlorinated pesticides regulated under EPA Method 8081
in 10 min
Low activity, decreased breakdown of sensitive pesticides
such as DDT
Provides robust column performance for high temperature
bake outs
MS Certified phases provide low bleed performance for
pesticide confirmation by MS
Column Profile
Low
Similar* to:
High
11
15
Polarity MR-1
Polarity MR-2
Bleed
Temperature Limits
Stability
Applications


(Isothermal/TPGC)
Rtx-CLPesticides
Stx-CLPesticides

Insecticides
Organophosphorous Pesticides
Aroclors/PCBs
Nitrogen Containing Pesticides

Haloacetic Acids
Herbicides
Multi-pesticide Residue Methods
Rtx-CLPesticides2
Stx-CLPesticides2
92
21,22
8,7
17, 16
18
10
30,32 35
39
29 31
36 37
28
34
11
33
12
4 5
6
2
19,20
7
26
27
10
9
2
19,23
24
25
14
3
21,22
MR-2
13
15
1
Baseline Resolution of
EPA Method 8081
20
38
14
Column: A) Zebron MultiResidue-2

B) Zebron MultiResidue-1
Dimensions: A) 30 meter x 0.32 mm x 0.25 m
B) 30 meter x 0.32 mm x 0.50 m
Part No.: A) 7HM-G017-11
B) 7HM-G016-17
Injection: On-Column @ 123C, 1 L
Oven Program: 120 C for 0.5 min to 210 C @ 30 C/min to
300 C @ 6 C/min for 2 min
Sample: All analytes are 50 ppb in isooctane
1. DBCP
22. trans-Nonachlor
2. Hexachlorocyclopentadiene
23. Endosulfan I
3. Etridiazole
24. DDE
4. Chloroneb
25. Dieldrin
5. Propachlor
26. Chloropropylate
6. Diallate
27. Chlorobenzilate
7. a-BHC
28. Endrin
8. Hexachlorobenzene
29. Nitrofen
9. g-BHC
30. DDD
10. b-BHC
31. Endosulfan II
11. Dichlone
32. Kepone
12. Alachlor
33. Trithion
13. d-BHC
34. Endrin aldehyde
14. Chlorothalonil
35. DDT
15. Heptachlor
36. Endosulfan sulfate
16. DCPA (Dacthal)
37. Captafol
17. Aldrin
38. Methoxychlor
39. Endrin ketone
19. Captan
40. Permethrin
20. trans-Chlordane (gamma)
41. Permethrin isomer
21. cis-Chlordane (alpha)
16
17
18
3
10
11
16
2425
23
39
3031 35
2829
36
34
33
3738
27 32
26
12
4
min
MR-1
14
13 15
40 41
12
5 6
6
10
12
14
40 41
16
18
App ID 16279
App ID 16280
EPA Methods 508 and 8081A
min
Well-suited for Dual Column Analysis
Injector
Zebron
MR-1
ECD Detector
Y-Splitter
5m
Z-Guard
ECD Detector
Zebron
MR-2

Phenomenex
Zebron GC Columns

ZB-MultiResidue 1 and 2 (contd)
Zebron ZB-MultiResidue GC Columns (MR)-1
ID(mm)
df(m)
Temp. LimitsC
20-Meter
0.18
0.18
-60 to 320/340
30-Meter
0.25
0.25
-60 to 320/340
0.32
0.25
-60 to 320/340
0.32
0.50
-60 to 320/340
0.53
0.50
-60 to 320/340
Zebron ZB-MultiResidue GC Columns (MR)-2
ID(mm)
df(m)
Temp. LimitsC
30-Meter
0.25
0.20
-60 to 320/340
0.32
0.25
-60 to 320/340
0.53
0.50
-60 to 320/340
Part No.
Price
7FD-G016-08
7HG-G016-11
7HM-G016-11
7HM-G016-17
7HK-G016-17
Part No.
Price
7HG-G017-10
7HM-G017-11
7HK-G017-17
Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G016-11-B or
7HG-G017-11-B. Some exceptions may apply. Agilent 6850 and some SRI and process GC systems
use only 5 in. cages. See p. 70.
0.25 mm ID
(kit consists of products below)
Part No.: KG0-8237
Description
Dimension
MultiResidue MR-1 Column
30 meter x 0.25 mm x 0.25 m df
Z-Guard
5 meter x 0.25 mm
Universal Capillary Column Y-connector, Borosilicate
0.32 mm ID
Part No.: KG0-8238
Description
Dimension
Z-Guard
5 meter x 0.32 mm
0.53 mm ID
Part No.: KG0-8239
Description
Dimension
Z-Guard
5 meter x 0.53 mm
Price:
Part No.
7HG-G016-11
7HG-G017-10
7AG-G000-00-GZ0
AG0-4717
Price:
Part No.
7HM-G016-17
7HM-G017-11
7AM-G000-00-GZ0
AG0-4717
Price:
Part No.
7HK-G016-17
7HK-G017-17
7AK-G000-00-GZ0
AG0-4717
Price
Price
ZB-MultiResidue Column Kits
Price


Phenomenex
93
Zebron GC Columns
Zebron ZB-Drug-1

2009 R&D 100

Award Recipient
~exclusive~
Optimized phase for the separation of drugs of abuse

Provides fast analysis with great peak shape
Improves resolution of target analytes from matrix
interferences
Specially deactivated to improve quantitation for drug
compounds
Applications
Drugs of Abuse

16733
Opiates on a ZB-5ms
Opiates on a ZB-Drug-1
17806
3,4
3,4
1,2
Unique Selectivity
for Drugs
4.8
4.7
4.9
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
5.1
5.2
5.3
5.4
5.5
5.6
5.7
5.8
5.9
6 min
17809
Amphetamines
7
4
3
1
App ID 17809
5
9
10
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
94
4.0
4.1
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-5ms
10 meter x 0.18 mm x 0.18 m
7CD-G018-08
Split 10:1 @ 240C, 1 L
140C to 320C @ 20C for 1 min
MSD @ 230C/min
Analytes @ 10 pppm BSTFA/Pyridine
1. Codeine-d6
2. Codeine
3. Morphine-d3
4. Morphine
App ID 17806
App ID 16733
Zebron ZB-Drug-1
10 meter x 0.18 mm x 0.18 m
7CD-G023-08
Split 10:1 @ 280C, 0.1 L
70C to 130C @ 20C/min to 270 C @ 30 C/min
MSD @ 230C
Analytes are 40 ppm derivatized with HFBA
1. Amphetamine-d11
6. MDA
2. Amphetamine
7. MDMA-d5
3. Methamphetamine-d14
8. MDMA
4. Methamphetamine
9. MDEA-d5
5. MDA-d5
10. MDEA
4.2
4.3
4.4
4.5
4.6
4.7
4.8
min
Zebron ZB-Drug-1
10 meter x 0.18 mm x 0.18 m
7CD-G023-08
Split 10:1 @ 280C, 1 L
180C to 340C @ 20C/min
MSD @ 230C
Analytes @ 10 pppm BSTFA/Pyridine
1. Codeine-d6
2. Codeine
3. Morphine-d3
4. Morphine
Zebron ZB-Drug-1 GC Columns
ID(mm)
df(m)
10-Meter
0.18
0.18
15-Meter
0.25
0.25
30-Meter
0.25
0.25
Temp. LimitsC
Part No.
40 to 320/340
7CD-G023-08
40 to 320/340
7EG-G023-11
40 to 320/340
7HG-G023-11
Price
See p. 70.
min
ZB-Drug-1 Test Mix

Part No.: AG0-8431
Price:


Phenomenex
Zebron GC Columns
ZB-BAC1 and ZB-BAC2

~exclusive~
Proprietary phase specially designed for blood

alcohol analysis
Enhanced accuracy for post mortem samples
Fast run time with baseline resolution of key components
in just 2 minutes
Applications
Blood Alcohol Analysis
Glycols
Abused Inhalant Anesthetics

Industrial Solvents
Enhanced resolution of ethanol and acetone peaks

Achieve confirmation with two elution order changes
when running columns in parallel
Allows for the use of t-butanol or n-propanol as an internal
standard
(Isothermal/TPGC)
Alternative to:
Rtx-BAC1
DB-ALC1
Rtx-BAC2
DB-ALC2
Blood Alcohols on Confirmation Column
17982
A
B
17983
2
2
6
7
8
Column: A) Zebron ZB-BAC1

B) Zebron ZB-BAC2
Dimensions: A) 30 meter x 0.53 mm x 3.00 m
B) 30 meter x 0.53 mm x 2.00 m
Part No.: A) 7HK-G021-36
B) 7HK-G022-32
Injection: Split 0.8:1 @ 150C, 1 mL
Carrier Gas: Helium @ 80 cm/sec (constant flow)
Oven Program: 40C (Isothermal)
Sample: Analytes 0.025% and internal standards 0.100% in water
1. Methanol
2. Acetaldehyde
3. Ethanol
4. Isopropanol
5. Acetone
6. t-Butanol (IS)
7. n-Propanol (IS)
8. 2-Butanol (IS)
min
7
8
App ID 17983
App ID 17982
min
Zebron ZB-BAC1 GC Columns
ID(mm)
df(m)
30-Meter
0.32
1.80
0.53
3.00
Zebron ZB-BAC2 GC Columns
ID(mm)
df(m)
30-Meter
0.32
1.20
0.53
2.00
Temp. LimitsC
Part No.
-20 to 260/280
-20 to 260/280
7HM-G021-31
7HK-G021-36
Temp. LimitsC
Part No.
-20 to 260/280
-20 to 260/280
7HM-G022-25
7HK-G022-32
Price
Blood Alcohols on Primary Column
Price
Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HM-G021-31-B or
7HM-G022-25-B. Some exceptions may apply. Agilent 6850 and some SRI and process GC systems
use only 5 in. cages. See p. 70.


Phenomenex
95
SGE GC Columns
forte BPX70
forte BPX90
Alternative to:
DB-23
SP-2330
Alternative to:
Rtx-2330
VF-23ms
007-23
AT-Silar
PE-23
CP-Sil 88
HP-88
Operating Temperatures
Applications
Fatty acid methyl esters
(FAMEs) CIS/TRANS
Carbohydrates
Pharmaceuticals
Film df(m):
0.25, 0.50
50C to 250C/260C
SGE GC Columns | GC COLUMNS & ACCESSORIES
Fragrances
Aromatics
Petrochemical
PCBs
FAMEs
Film df(m):
Temp Limit:
0.25 - 0.50
80C to 280C
df(m)
Part No.
Mfr. No.
0.25
0.25
0.50
CG0-5507
CG0-5516
CG0-5522
054602
054606
054610
0.25
0.25
0.25
0.50
CG0-5508
CG0-5512
CG0-5517
CG0-5523
054612
054622
054616
054620
0.25
0.25
CG0-5509
CG0-5518
054603
054607
0.25
0.25
CG0-5513
CG0-5519
054623
054617
Price
BPX90
ID(mm)
30-Meter
0.25
0.32
df(m)
Part No.
Mfr. No.
0.25
0.50
CG0-8229
CG0-8230
054580
054583
Price
forte HT8
Applications

PCB Analyses
Nitro-substituted Aromatics
Pesticides/herbicides
Film df(m):
0.25, 0.50
Temp Limit:
-20C to 360C/370C
forte SolGel-Wax
Applications
Alcohols
Ketones
Aldehydes
Film df(m):
Temp Limit:
0.25 - 1.00
30C to 300C
SolGel-WAX
ID(mm)
30-Meter
0.25
0.32
0.53
df(m)
Part No.
Mfr. No.
0.25
0.50
1.00
CG0-8234
CG0-8235
CG0-8236
054796
054797
054785
All other SGE GC columns not listed are available.
96
SP-2380
SP-2560
Applications
Temp Limit:
BPX70
ID(mm)
25-Meter
0.22
0.32
0.53
30-Meter
0.22
0.25
0.32
0.53
50-Meter
0.22
0.32
60-Meter
0.25
0.32
Rtx-2560
SP-2340
Price
HT8
ID(mm)
12-Meter
0.32
25-Meter
0.22
0.32
0.53
df(m)
Part No.
Mfr. No.
0.25
CG0-5555
054679
0.25
0.25
0.50
CG0-5553
CG0-5556
CG0-5558
054675
054680
054685
Price
forte BPX-Volatiles
Applications
EPA Method 624, 502.2, 8260
Film df(m):
1.0 - 3.0
Temp Limit:
0C to 290C/300C
BPX-Volatiles
ID(mm)
30-Meter
0.25
0.32
0.53
df(m)
Part No.
Mfr. No.
1.40
1.80
3.00
CG0-8231
CG0-8232
CG0-8233
054980
054982
054984
Price
Phenomenex
Quadrex GC Columns
PLOT and Capillary GC Columns available from Phenomenex

PLT-5A, Molecular Sieve 5A PLOT
Reliable permanent gas analysis
007-225, (50%-Cyanopropylphenyl) Methylpolysiloxane

Resolve cis/trans fatty acids
Excellent thermal stability
Good for separation of carbohydrates
Separates O2 /Ar at subambient conditions

PLT-5A, Molecular Sieve 5A PLOT
ID(mm)
df(m)
30-Meter
0.53
25.0
Part No.
Mfr. No.
CG0-5151
QPLT-5A-30V
Price
PHAT Phases
(Polymer Height Augmentation Technology)
Increased retention for low boiling samples
Can be used as a replacement for many PLOT columns
Increased loadability
Available on Phases 007-1 and 007-5
007-5 PHAT Phases (Polymer Height Augmentation Technology)

ID(mm)
df(m)
Part No.
Mfr. No.
15-Meter
0.25
8.00
CG0-4920
Q-5-15-8.0F
20-Meter
0.18
6.00
CG0-7664
Q-5-20HS-6.0F
30-Meter
0.25
8.00
CG0-4921
Q-5-30-5.0F
0.32
10.00
CG0-7665
Q-5-30W-10.0F
0.53
18.00
CG0-4963
Q-5-30V-18.0F
Price
Mfr. No.
Price
Q225-15-0.1F
Q225-15-0.25F
Q225-15W-0.25F
Q225-15V-0.1F
Q225-30-0.1F
Q225-30-0.25F
Q225-30-0.5F
Q225-30W-0.1F
Q225-30W-0.25F
Q225-30W-0.5F
Q225-30V-0.1F
Q225-30V-0.25F
Q225-30V-0.5F
Q225-30V-1.0F
007-65HT (65%-Phenyl) Methylpolysiloxane

Fine tuned for the analysis for triglycerides
Available in higher temperature aluminum-clad tubing
Price
007-65HT (65%-Phenyl) Methylpolysiloxane

ID(mm)
df(m)
Part No.
15-Meter
0.25
0.10
CG0-7666
0.25
0.25
CG0-7667
30-Meter
0.25
0.10
CG0-7668
0.25
0.25
CG0-7669
Mfr. No.
Price
Q-65HT-15-0.1F
Q-65HT-15-0.25F
Q-65HT-30-0.1F
Q-65HT-30-0.25F
BTR-CW, Polyethylene Glycol (PEG)-Oxygen Resistant

Oxygen resistant phase
Bonded and crosslinked
007-502 Cyanopropylphenyl Methylpolysiloxane

Specially designed for EPA Method 502.2
Excellent thermal stability
Similar to Rtx-502.2
007-502 Cyanopropylphenyl Methylpolysiloxane
ID(mm)
df(m)
Part No.
60-Meter
0.53
3.00
CG0-5012
75-Meter
0.53
3.00
CG0-5013
105-Meter
0.53
3.00
CG0-5014
GC COLUMNS & ACCESSORIES | QUADREX GC Columns
007-1 PHAT Phases (Polymer Height Augmentation Technology)

ID(mm)
df(m)
Part No.
Mfr. No.
15-Meter
0.25
8.00
CG0-4857
Q-1-15-8.0F
20-Meter
0.18
6.00
CG0-7662
Q-1-20HS-6.0F
30-Meter
0.25
8.00
CG0-4858
Q-1-30-5.0F
0.32
10.00
CG0-7663
Q-1-30W-10.0F
0.53
18.00
CG0-4900
Q-1-30V-18.0F
007-225, (50%-Cyanopropylphenyl) Methylpolysiloxane

ID(mm)
df(m)
Part No.
15-Meter
0.25
0.10
CG0-5048
0.25
0.25
CG0-5051
0.32
0.25
CG0-5060
0.53
0.10
CG0-5066
30-Meter
0.25
0.10
CG0-5049
0.25
0.25
CG0-5052
0.25
0.50
CG0-5055
0.32
0.10
CG0-5058
0.32
0.25
CG0-5061
0.32
0.50
CG0-5064
0.53
0.10
CG0-5067
0.53
0.25
CG0-5070
0.53
0.50
CG0-5073
0.53
1.00
CG0-5076
Mfr. No.
Q-502-60V-3.0F
Q-502-75V-3.0F
Q-502-105V-3.0F
Price
BTR-CW, Polyethylene Glycol (PEG)-Oxygen Resistant

ID(mm)
df(m)
Part No.
Mfr. No.
30-Meter
0.32
1.00
CG0-5097
QBTR-CW-30W-1.0F
0.53
3.00
CG0-5112
QBTR-CW-30V-3.0F
60-Meter
0.25
0.25
CG0-5083
QBTR-CW-60-0.25F
0.25
0.50
CG0-5086
QBTR-CW-60-0.5F
0.32
0.25
CG0-5095
QBTR-CW-60W-0.5F
Price
(1) All other Quadrex GC Columns not listed are available.

(2) Phenomenex listing of Quadrex Mfr. Nos. begin with Q instead of 007 as used by manufacturer.
Phenomenex
97
GC Columns Check Standards

Test the Performance of GC Columns
Convenient way to check column performance
Essential tool for GC troubleshooting
Affordable and easy to use
Sealed in 2 mL glass ampules prevent evaporation and
increase shelf life
Zebron ZB-50
AG0-5157
250 g/mL each in hexane:
Part No.:

1.
2.
3.
4.
Undecane
Tridecane
2,4-Dimethylphenol
2,6-Dimethylaniline
Price:
5. 1-Undecanol
7. Hexadecane
1. Decane
3. 4-Chlorophenol
4. Naphthalene
Price:
5. Tridecane
6. 1-Undecanol
8. Pentadecane
Zebron ZB-1, ZB-5, ZB-1HT, and ZB-5HT

AG0-5155
Part No.:
1. Undecane
2. 4-Chlorophenol
3. Tridecane
Price:
4. 1-Undecanol
6. Pentadecane
Guardian Integrated Guard / ZB-5
1.
2.
3.
4.
5.
Decane
2-Ethylhexanoic Acid
1,6-Hexanediol
4-Chlorophenol
Tridecane
6.
7.
8.
9.
1-Methylnaphthalene
1-Undecanol
Tetradecane
Dicyclohexylamine
Zebron ZB-5MSi
1.
2.
3.
4.
5.
Decane
Methyl Caprylate
1,6-Hexanediol
4-Chlorophenol
Tridecane
AG0-7869

1. 2-Octanone
2. Tetradecane
3. Pentadecane
4. 1-Octanol
5. Hexadecane
4. Chlorobenzene
5. Nonane
Price:
6. Methyl decanoate
7. Heptadecane
8. Methyl undecanoate
9. 1-Decanol
10. Octadecane
11. Methyl dodecanoate

13. Nonadecane
Zebron ZB-WAX and ZB-FFAP

AG0-5158
Part No.:

1.
2.
3.
4.
5.
2-Octanone
Tetradecane
1-Octanol
Methyl decanoate
Methyl undecanoate
Price:
6.
7.
8.
9.
1-Decanol
Methyl dodecanoate
2,6-Dimethylaniline
2,6-Dimethylphenol
Price:
6. 1,8-Octanediol
7. Dihexylamine
8. 1-Undecanol
10. Pentadecane
App ID 18461
Price:
Zebron ZB-WAXplus
Part No.:
AG0-8431
250 g/mL each in acetone:
App ID 19305
AG0-8362
Part No.:
AG0-5159
1000 g/mL each in methanol:
Price:
App ID 14326, App ID 5164
AG0-7549
Part No.:
Zebron ZB-624
Part No.:
1. 1,2-Dichloropropane
2. Octane
3. Tetrachloroethylene
App ID 16214
AG0-7805
App ID 5165
Zebron ZB-1ms
Part No.:
AG0-8645
Zebron ZB-Drug-1
Part No.:

1.
2.
3.
4.
Dodecane
Tridecane
4-Chlorophenol
Tetradecane
Price:
5. 1-Undecanol
8. Hexadecane
Zebron ZB-5ms, ZB-XLB, and ZB-XLB-HT

AG0-7578
Part No.:

1.
2.
3.
4.
5.
Decane
2-Ethylhexanoic Acid
1,6-Hexanediol
4-Chlorophenol
Tridecane
Price:
7. 1-Undecanol
8. Tetradecane
10. Pentadecane
Zebron ZB-35, ZB-35HT, ZB-1701, and ZB-1701P

AG0-5156
Part No.:

1.
2.
3.
4.
Undecane
2,4-Dimethylphenol
2,6-Dimethylaniline
Tetradecane
Price:
5. 1-Undecanol
7. Hexadecane
App ID 5158

App ID 14836
App ID 16439
App ID 14973
GC COLUMNS CHECK STANDARDS | GC COLUMNS & ACCESSORIES
App ID 15840
All test mixes supplied with Certificate of Analysis
App ID 5163
Suitable for Phenomenex Zebron and equivalent brands

Part No.:
1. Undecane
2. Dodecane
3. Tridecane
Price:
4. 1-Undecanol
6. Pentadecane
Grob Test Mixture

(For most GC capillary columns)
Part No.: AG0-5154
Price:
400 g/mL each in methylene chloride:

1.
2.
3.
4.
2,3-Butanediol
Decane
Undecane
1-Octanol
5.
6.
7.
8.
1-Nonanal
2-Ethylhexanoic acid
2,6-Dimethylphenol
2,6-Dimethylaniline
9.
10.
11.
12.
Methyl decanoate
Methyl undecanoate
Dicyclohexylamine
Methyl dodecanoate
Test mix components are shown in order of elution
98
Phenomenex
GC Accessories
Capillary Unions, Mini-Unions, and Splitters
Selection Guide
Use the Union or Mini-Union for:
Use the Y-Connector (splitter) for:
Splitting a sample onto two columns (perform

confirmational analysis in a single injection)
Splitting the column eluent to two detectors
Connecting a guard column to an analytical column

Connecting columns of different selectivities
Connecting transfer lines to, e.g., mass spec
Repairing a broken column
Capillary Mini-Unions
Capillary Unions and Splitters
High-precision unions for connecting capillary GC columns

of same or dissimilar sizes
Connect fused silica capillary tubing of the same

or different diameter, from 0.10 to 0.53 mm ID
Inert and precise glass-lined bore
Patented laser-formed linear taper
Low dead volume
Provides leak-free seal without tools, glue, or fittings
Comes with 1 mini-union, 2 nuts, and 5 ferrules
Stays sealed even at high temperatures and pressures

Laser-cut smooth ends prevent column damage
during insertion
Graphite/Vespel Ferrule Mini-Unions

15 % Graphite / 85 % Vespel ferrules, Rated to 350C
Part No.
AG0-5160
AG0-5161
AG0-5162
Unit
ea
ea
ea
Price
Part No.
AG0-7033
AG0-7034
AG0-7035
Unit
10/pk
10/pk
10/pk
Price
Capillary Unions and Splitters
Part No.
Description
AG0-4716
Universal Capillary Column Union, Borosilicate
AG0-4717
Unit
5/pk
ea
Price
SilTite Mini-Unions
Supplied with SilTite ferrules no more leaks and no need
to re-tighten after installation
Recommended for high temperature analysis. Stable above
450C.
SilTite Mini-Unions
Column 1 Column 2 ID Ferrule ID
ID (mm)
(mm)
(mm)
0.10-0.25 0.10-0.53
0.4
0.28-0.35 0.32-0.53
0.5
0.45-0.53 0.45-0.53
0.8
Replacement Ferrules
Ferrule ID
Column ID (mm)
(mm)
0.10-0.25
0.4
0.28-0.35
0.5
0.45-0.53
0.8
Similar to Mfr.
No.*
073550
073551
073554
Part No.
AG0-8763
AG0-8764
AG0-8825
Unit
ea
ea
ea
Similar to Mfr.
No.*
073470
073471
073473
Part No.
AG0-8759
AG0-8760
AG0-8824
Unit Price
10/pk
10/pk
10/pk
Price
Polyimide Resins
Permanently connects unions and splitters
to capillary tubing
Prevents connections from dislodging due
to vibration or shock
Polyimide Resins
Part No.
Description
AG0-5722
Polyimide Resin, 350 C, 0.5 mL
AG0-8514
High Temperature, 400 C, Polymide Resin, 0.5 mL
Unit
ea
ea
GC COLUMNS & ACCESSORIES | unions / splitters / polyimide resin
Graphite/Vespel Ferrule Mini-Unions
Column 1 Column 2 Ferrule ID Similar to Mfr.
ID (mm)
ID (mm)
(mm)
No.*
0.10-0.25 0.10-0.53
0.4
103431
0.28-0.35 0.32-0.53
0.5
103432
0.45-0.53 0.45-0.53
0.8
103433
Ferrule ID Similar to Mfr.
Column ID (mm)
(mm)
No.*
0.10-0.25
0.4
072696
0.28-0.35
0.5
072697
0.45-0.53
0.8
072698
Price
* Similiar to but not always an exact equivalent to the original manufacturers product.
For GC Retention Gaps and Guard Column Kits, see p. 68
Phenomenex
99
GC Accessories
Inlet Base Seals
Easy Seals for Agilent GCs ~exclusive~
Soft surface eliminates need for washer
Minimal torque required to form tight seal
Increase column lifetime with tight seal
Phenomenexs Easy Seals make a tight seal without the need to
apply high torque. These seals eliminate the need to use a washer
and reduce breakdown and adsorption of active compounds, such
as acids, bases, and pesticides.
Easy Seals
Part No.
Description
Standard, single groove for splitless applications, 0.8 mm dia. inlet hole
AG0-8619
Easy Seal Inlet Base Seal, Gold Plated, for Agilent GCs
AG0-8620
Easy Seal Inlet Base Seal, Gold Plated, for Agilent GCs
Unit
Price
2/pk
10/pk
Gold Inlet Base Seals for Agilent GCs

Traps non-volatile residues, septum fragments, and other contaminants
INLET BASE SEALS | GC COLUMNS & ACCESSORIES
Exceptional inertness
Gold Inlet Base Seals
Part No.
Description
AG0-7518
Gold Inlet Base Seal, splitless (single groove), 0.8 mm
AG0-7519
High Split Flow, cross groove for split applications, 0.8 mm dia. inlet hole
AG0-7520
Gold Inlet Base Seal, split (double groove/cross), 0.8 mm
AG0-7521
AG0-8581
AG0-8582
AG0-8583
AG0-8584
Replacement Inlet Seal Washers
AG0-8397
Inlet Seal Washers Gold Plated, for Agilent GC injection port
Similar to Mfr. No.*
Unit
18740-20885
18740-20885
2/pk
10/pk
5182-9652
5182-9652
2/pk
10/pk
21305
21306
2/pk
10/pk
21009
21010
2/pk
10/pk
12/pk
Price
Due to different replacement

frequencies, inlet seal washers
are sold separately from inlet
base seals.
Stainless Steel Inlet Base Seals for Agilent GCs

Lower cost alternative
Good for hydrocarbon analysis
Stainless Steel Inlet Base Seals
Part No.
Description
AG0-8393
Stainless Steel Inlet Base Seal, splitless (single groove), 0.8 mm
18740-20880
AG0-8394
Stainless Steel Inlet Base Seal, splitless (single groove), 0.8 mm
18740-20880
AG0-8395
Stainless Steel Inlet Base Seal, split (double groove/cross), 0.8 mm
AG0-8396
Stainless Steel Inlet Base Seal, split (double groove/cross), 0.8 mm
Replacement Inlet Seal Washers

AG0-7522
Inlet Seal Washers, Stainless Steel, for Agilent GC injection port
5061-5869
For Sealing O-Rings, see p. 107
100
Unit
Price
2/pk
10/pk
2/pk
10/pk
12/pk
*Similar to but not always an
exact equivalent to the original
manufacturer's product.
Phenomenex
GC Accessories
Installation Nuts
Quick and Easy!
~exclusive~
Cool-Lock Nut
Fast GC Column Installation without the Burn
Avoid burning your fingers againcools with the oven
Increased reproducibilitylocks insertion depth before installation
No need for wrench with hand-tightened connections
Part No. AGO-8319
No more Wite-Out, Tipp-Ex, or septa pieces!

Patent pending design
Cool-Lock Parts for Agilent GC Systems
Cool-Lock Parts for Shimadzu GC Systems
Cool-Lock GC Capillary Nut For Agilent GC Systems*
Part No.
Description
AG0-8319
Cool-Lock GC Capillary Nut For Use With
Short-Style Ferrules
AG0-8320
Long-Style Ferrules
Unit
ea
Price
ea
Cool-Lock GC Capillary Nut For Shimadzu GC Systems**

Part No.
Description
AG0-8419
Shimadzu GC Systems
Unit
ea
Price
** Guaranteed fit for Shimadzu 2010 and 2014 GC systems
Cool-Lock Nut Installation Gauge for Agilent GC Systems

Part No.
Description
AG0-8349
Cool-Lock Nut Installation Gauge
Unit
ea
Price
Cool-Lock Nut Installation Gauge For Shimadzu GC Systems

Part No.
Description
AG0-8420
Cool-Lock Nut Installation Gauge
Unit
ea
Price
How to Select a Cool-Lock Nut for your Agilent GC System
Decide on The Style of Cool-Lock Nut Needed

Short Style (AG0-8319)
Long Style (AG0-8320)
Recommended for use with standard short Recommend for use with standard long
style ferrules.
style ferrules.
Also, use for both the inlet and detector configurations on Agilent 6890, 5890, and 7890
GC systems (Short ferrules provide a robust
seal and typically remain tight over time).
Determine How Many Cool-Lock Nuts Needed Per System

Detector
MS
FID, ECD, Other
Number of Nuts
1
2
Standard Installation Nut

For capillary column connections to both Agilent
injectors and GC detectors (not MS)
GC COLUMNS & ACCESSORIES | Installation nuts
*Guaranteed fit for Aglilent 5850, 5890, 6850, 6890, 7890 GC systems
Interchangeable with original Agilent nuts

Standard length nut uses short Agilent ferrules (1.65 mm)
Extended length (deep well) nut uses standard
ferrules (2.4 mm)
GC Fittings
Part No.
AG0-5152
For Ferrule Selection Guide for

Cool-Lock Nuts, see p. 102
AG0-5153
Description
Agilent GC Standard Capillary Nut,
1/16 in. hex stainless steel, uses short
Agilent ferrules (1.65 mm length)
Agilent GC Deep Well Capillary Nut,
1/16 in. hex stainless steel, uses standard
Agilent ferrules (2.4 mm length)
Similar to
Mfr. No.*
5181-8830
Unit
2/pk
5020-8292
2/pk
Price
* Similiar to but not always an exact equivalent to the original manufacturer's product.
Caution: For safety, please use common sense when handling metal surfaces within the GC oven, including the Cool-Lock Nut. The Cool-Lock Nut is designed to track the GC oven temperature as close as
possible, therefore, when you cool down your GC oven, the Cool-Lock Nut will follow suit because it is related to oven temperature with the fan on. So if the oven is hot enough to cause severe burns, the CoolLock Nut will also be hot enough to cause severe burns. If the GC oven fan has turned off and the injection port temperature is still hot, the Cool-Lock Nut will begin to heat up causing it to be too hot to touch
with the bare hand. For any questions regarding use of the Cool-Lock Nut, please contact your local Phenomenex representative.
Phenomenex
101
GC Accessories
Ferrules
Ferrule Selection Guide by Material
Material
Advantage
Disadvantage
Recommended for
100 % Graphite
Easy to use
Tight, stable seal
Rated to 450C
Porous to oxygen
Not for MS or other oxygen sensitive detectors
Easily deformed
Potential to contaminate system
General use
FID and NPD
High temperature analysis
Cool on-column
85/15 % Vespel/Graphite
Durable for long lifetime

Not porous to oxygen
Rated to 350C
Non-reusable
Need to re-tighten frequently
Flows at high temperatures
Good for MS or other oxygen sensitive detectors

Most leak free
60/40 % Vespel/Graphite
Easier to use than 85/15

Not porous to oxygen
Rated to 400C
Non-reusable
Easier to deform than 85/15
Good for MS or other oxygen sensitive detectors

Best balance between tight seal and ease of use
No need to re-tighten
Reliable seal
No contamination
Rated > 450C
Easily deforms
High temperature MS analysis
SilTite
ferrules | GC COLUMNS & ACCESSORIES
Ferrule Selection Guide by Length

Length
Advantage
Disadvantage
Recommended for
Short
Robust seal
Not recommended for MS detector connection
Standard detectors and inlet
Long
Good nut and interface design
Not recommended for inlet connection
MS detector connection
Ferrule Selection Guide for Cool-Lock Nuts

Column ID (mm)
0.10-0.25
0.25-0.35
0.45-0.53
Ferrule ID (mm)
0.4
0.5
0.8
Short Ferrules
Composition
GC Column ID (mm)
0.10-0.25
100 %
Graphite
85 % Vespel /
15 % Graphite
Agilent Systems
Long Style Nut
Short Style Nut
Top Ferrule
Bottom Ferrule
Top Ferrule
Bottom Ferrule
AG0-4698
AG0-4698
AG0-7513
AG0-4698
AG0-4701
AG0-4701
AG0-7513
AG0-4701
AG0-4704
AG0-4704
AG0-8676
AG0-4704
Ferrule ID (mm)
0.4
0.28-0.35
0.5
0.45-0.53
0.8
0.10-0.25
0.4
0.28-0.35
0.5
0.40-0.53
0.8
Shimadzu Systems
Top Ferrule
AG0-8881
AG0-8881
AG0-8882

500-2114
072635
5080-8853
072636
500-2118
5181-3323
5181-3322
Part No.
AG0-8929
Preconditioned
N
Unit
10/pk
AG0-7513
10/pk
AG0-8676
10/pk
AG0-7318
AG0-7321
N
Y
10/pk
10/pk
5062-3514
5062-3513
5062-3512
5062-3511
AG0-7319
AG0-7322
AG0-7320
AG0-7323
N
Y
N
Y
10/pk
10/pk
10/pk
10/pk
Bottom Ferrule
AG0-4698
AG0-4701
AG0-4704
Price
All ferrules are 1/16 in. (except SilTite)

Preconditioned for lower bleed.
102
All Vespel containing ferrules should be pre-shrunk

in an oven at 250C for at least 4 hours prior to use.
Phenomenex
GC Accessories
Ferrules (contd)
Long Ferrules
Composition
GC Column ID (mm)
0.10-0.25
100 % Graphite
0.28-0.35
0.45-0.53
85 % Vespel /
15 % Graphite
60 % Vespel /
40 % Graphite
Ferrule ID (mm)
0.4

20200
Part No.
AG0-4698
20227
AG0-4699
72635
AG0-4701
AG0-4702
82636
AG0-4704
AG0-4705
0.5
0.8
0.10-0.25
0.4
0.28-0.35
0.5
0.45-0.53
0.8
0.10-0.25
0.4
0.28-0.35
0.5
0.45-0.53
0.8
07663
5062-3508
072654
5062-3506
072655
5062-3538
Preconditioned
N
N
N
Unit
10/pk
50/pk
10/pk
50/pk
10/pk
50/pk
AG0-8677
10/pk
AG0-8678
10/pk
AG0-8679
10/pk
20211
AG0-4707
20229
AG0-4708
20212
AG0-4710
20231
AG0-4711
Price
10/pk
50/pk
10/pk
50/pk
20213
AG0-4713
20230
AG0-4714
50/pk

072662
5062-3580
Part No.
Preconditioned
Unit
AG0-8680
10/pk
AG0-8681
10/pk
10/pk
85 % Vespel /
15 % Graphite
Ferrule ID (mm)
0.10-0.25
0.4
0.28-0.35
0.5
0.45-0.53
0.8
072674
AG0-8682
10/pk
Ferrule ID (mm)
0.4
0.5
0.8

073220
073221
073222
Part No.
AG0-8762
AG0-8757
AG0-8758
Preconditioned
Y
Y
Y
Unit
10/pk
10/pk
10/pk
SilTite Ferrules
Composition
GC Column ID (mm)
0.10-0.25
SilTite
0.28-0.35
0.45-0.53
212222
5062-3581
Price
GC COLUMNS & ACCESSORIES | ferrules
Two Hole Ferrules
Composition
GC Column ID (mm)
Price
Note: SilTite ferrules are to be used with SilTite nuts. Please contact your Phenomenex technical consultant or distributor for the appropriate nut and ferrule part numbers for your GC system.
100% Graphite Ferrules (Metal Encapsulated) for Shimadzu GCs
Composition
GC Column ID (mm) Ferrule ID (mm) Similar to Mfr. No.*
100% Graphite
0.10-0.25
0.4
221-32126-05
(Metal Encapsulated)
0.25-0.35
0.5
221-32126-05
0.45-0.53
0.8
221-32126-08
Part No.
AG0-8881
AG0-8881
AG0-8882
Preconditioned
Y
Y
Y
Unit
10/pk
10/pk
10/pk
Price
For Replacement Ferrules for Mini-unions, see p. 99
All ferrules are 1/16 in. (except SilTite)

Preconditioned for lower bleed.
Phenomenex
All Vespel containing ferrules should be pre-shrunk

in an oven at 250C for at least 4 hours prior to use.
103
GC Accessories
Injection Port Inlet Liners
Liners for Agilent Technologies (HP) GC Systems
Liners
Description
Split / Splitless Liner with wool
Splitless Single Taper Liner with wool
Cup Splitter / Split Liner*
Cup Splitter / Split Liner with wool*
Splitless Liner Straight Liner
Splitless Straight Liner*
Split / Splitless, Single Taper Liner*
Direct Liner
Column Installs This End
Inlet Liners | GC COLUMNS & ACCESSORIES
Split / Splitless, Single Taper Liner
Agilent Technologies (HP)
Benefits / Uses
5880/5890/
6890/7890
Large injection, trace

analysis
5880/5890/
6890/7890
High and low MW

compounds and large
volume injections
5880/5890/
6890/7890
Large volume injection

of dirty samples
5880/5890/
6890/7890
Small injection, trace

analysis
5880/5890/
6890/7890

analysis
5880/5890/
6890/7890

analysis
5880/5890/
6890/7890

analysis
5880/5890/
6890/7890
Injection < 1 L
or Purge & Trap/
Headspace
5880/5890/
6890/7890
Split / Splitless, Recessed Gooseneck Liner with wool Large injection of dirty
samples
Single Taper Direct Connect with Side Hole (top)
5880/5890/
6890/7890
Similar to Mfr. No.**
ea
19251-60540
5183-4691
5/pk
5183-4692
25/pk
4 x 78.5 x 6.45
ea
5062-3587
5183-4693
5/pk
5183-4694
25/pk
4 x 78.5 x 6.3
ea
HP 18740-80190
5183-4699
5/pk
5183-4700
25/pk
4 x 78.5 x 6.3
ea
18740-60840
5183-4697
5/pk
5183-4698
25/pk
2048205
Supelco
2 x 78.5 x 6.45 not deactivated HP 18740-80220
5181-8818
ea
5183-4703
5/pk
5183-4704
25/pk
4 x 78.5 x 6.3
ea
210-3003
210-3003-5
5/pk
25/pk
4 x 78.5 x 6.45
ea
5181-3316
5183-4695
5/pk
5183-4696
25/pk
2 x 78.5 x 6.45
ea
5/pk
25/pk
1.5 x 78.5 x 6.45

ea
18740-80200
5183-4709
5/pk
5183-4710
25/pk
4 x 78.5 x 6.45
ea
19251-60540
ea
5183-4647
5/pk
5183-4691
25/pk
5183-4692
4 x 78.5 x 6.3
ea
G1544
5/pk
25/pk
5880/5890/
6890/7890
5880/5890/
6890/7890
4 x 78.5 x 6.3
Split Liner with wool
General use or dirty

samples
5880/5890/
6890/7890
4 x 78.5 x 6.3
Split / Splitless, Double Taper/Gooseneck Liner

analysis of active
compounds
5880/5890/
6890/7890
4 x 78.5 x 6.3
Split / Splitless FocusLiner with wool
Trace analysis of dirty

samples
5880/5890/
6890/7890
4 x 78.5 x 6.3
Split / Splitless, FocusLiner Single Taper

with wool

samples
5880/5890/
6890/7890
4 x 78.5 x 6.3
Split / Splitless, FocusLiner Single Taper with wool

samples
5880/5890/
6890/7890
Double Gooseneck with Bottom Hole
Great recovery and

linearity for trace
analysis of active
compounds
Splitless, Single Taper Liner with wool
Single Taper Direct Connect with Side Hole (bottom)
Units
4 x 78.5 x 6.3
Great recovery and

linearity for trace
analysis of active
compounds
Great recovery and
linearity for trace
analysis of active
compounds
ea
5/pk
G1544-80730
20712
20713
20714
20772
20773
20774
20798
20799
20800
20795
20796
20797
20972
20973
22408
22409
22410
21054
21055
20998
Part No.
AG0-4655
AG0-4656
AG0-4657
AG0-4658
AG0-4647
AG0-4648
AG0-7853
AG0-4649
AG0-4650
AG0-4651
AG0-4652
AG0-7516
AG0-7640
AG0-4653
AG0-4654
AG0-4659
AG0-4660
AG0-4661
AG0-4662
AG0-7850
20756
20771
AG0-7851
AG0-8172 5/pk
AG0-8173 5/pk
AG0-7515 5/pk
AG0-7582 25/pk
AG0-4680 5/pk
AG0-7514 25/pk
20782
20783
22405
22406
22407
20709
20710
Unit
5/pk
25/pk
5/pk
25/pk
5/pk
5/pk
25/pk
5/pk
25/pk
5/pk
25/pk
5/pk
25/pk
5/pk
25/pk
5/pk
25/pk
5/pk
5/pk
5183-4647
5183-4701
5183-4702
5183-4711
5183-4712
5183-4713
21032
21033
20784
20785
20786
21022
21023
20979
20994
20995
20996
4 x 78.5 x 6.3
5/pk
092003
22406
AG0-8175
5/pk
5880/5890/
6890/7890
4 x 78.5 x 6.3
ea
5/pk
G1544-80700
20989
20954
AG0-8430
5/pk

analysis
5880/5890/
6890/7890
4 x 78.5 x 6.45
5/pk
5183-4693
AG0-8499
5/pk

analysis
5880/5890/
6890/7890
4 x 78.5 x 6.3
5/pk
25/pk
5183-4691
5183-4692
AG0-8653 5/pk
AG0-8654 25/pk

analysis of dirty
samples
5880/5890/
6890/7890
2.3 x 78.5 x 6.3
5/pk
210-4022
AG0-8379
5181-3315
5183-4705
5183-4706
210-4004
Price
5/pk
25/pk
ea
5/pk
25/pk
ea
5/pk
25/pk
ea
5/pk
25/pk
ea
5/pk
25/pk
Note: Large injection 2 L. Small injection 2 L *Liner is not deactivated
104
GC Model No.

analysis
Dimensions
ID x L x OD
(mm)
5/pk
** Similar to but not always an exact equivalent to the original manufacturers product.
Phenomenex
GC Accessories
Injection Port Inlet Liners (contd)
Liners for PerkinElmer GC Systems
Liners
PerkinElmer
Units
Part No.
AG0-4681
AG0-7864
Unit
5/pk
25/pk
092092
09209225
AG0-7639
AG0-7865
5/pk
25/pk
3.5 x 100 x 5
5/pk
0330-5181
20740
AG0-4663
5/pk
AutoSystem
4 x 92 x 6.2
5/pk
N610-1052 20833214.5
AG0-4665
5/pk

analysis
8000/Sigma
3 x 100 x 5
5/pk
092091
AG0-8655
5/pk

analysis
AutoSystem &
Clarus 500
4 x 92 x 6.2
5/pk
092095
AG0-8656
5/pk
General use or dirty samples AutoSystem &

Clarus 500
(PSS Injector)
Large Volume Injection (LVI) liner for AutoSystem XL Large injection, trace
AutoSystem &
analysis
(PSS injector), sintered glass
Clarus 500
(PSS Injector)
2 x 86.2 x 4
5/pk
092101
AG0-8657
5/pk
2 x 86.2 x 4
5/pk
092244
AG0-8658
5/pk
Split, Straight Liner

analysis
AutoSystem /
Clarus 500
4 x 92 x 6.2
5/pk
092100
AG0-8659
5/pk

analysis
AutoSystem /
Clarus 500
2 x 92 x 6.2
5/pk
092103
AG0-8660
5/pk
Benefits / Uses
GC Model No.
Split / Splitless Single Taper / Gooseneck Tapered

FocusLiner with wool
General use or dirty samples AutoSystem &

Clarus 500
Split / Splitless Tapered FocusLiner with wool
Dirty samples, trace samples AutoSystem &

Clarus 500
4 x 92 x 6.2
Cup Splitter / Split Liner*
Low activity, great for large

volume injections
8000/Sigma
Split / Splitless Liner*
General use and trace

samples
3 mm ID Split / Splitless
Split / Splitless Single Taper FocusLiner
Split / Splitless FocusLiner with wool
Price
Note: Large injection 2 L. Small injection 2 L *Liner is not deactivated ** Similar to but not always an exact equivalent to the original manufacturers product.
Liners for Shimadzu GC Systems

Liners
Shimadzu
Units
Great recovery and linearity

for trace analysis of active
compounds
17A, 17B, 2010,

2014
3.4 x 95 x 5
092068
Part No.
AG0-4683
Unit
Split/Splitless Single Taper / Gooseneck Tapered

Splitless Straight Liner

analysis
17A, 17B, 2010,

2014
2.6 x 95 x 5
AG0-4667
5/pk
Splitless Liner
Trace analysis
9A/15A/16A
3.4 x 139 x 5
5/pk
25/pk
20749
20750
AG0-4669
5/pk
Split / Splitless, Single Focus Taper Liner
General use or dirty samples
14/15A/16
3.4 x 99 x 5
5/pk
092066
AG0-4682
5/pk
Split / Splitless, Liner with Middle Gooseneck
17A/2014
3.4 x 95 x 5
5/pk
092085
AG0-8661
5/pk
Split / Splitless, FocusLiner with wool

analysis
17A/2014
3.4 x 95 x 5
5/pk
092062
AG0-8662
5/pk
Split / Splitless, Recessed Gooseneck Liner with

quartz wool
17A/2014
3.4 x 95 x 5
5/pk
092061
AG0-8663
5/pk
Split / Splitless, Single Taper FocusLiner
2010
3.4 x 95 x 5
5/pk
092058
AG0-8664
5/pk
Description
Dimensions
ID x L x OD
(mm)
Benefits / Uses
GC Model No.
Price
GC COLUMNS & ACCESSORIES | Inlet Liners
092095
09209525
Description
Dimensions
ID x L x OD
(mm)
4 x 92 x 6.2
5/pk
Note: Large injection 2 L. Small injection 2 L ** Similar to but not always an exact equivalent to the original manufacturers product.
Contact Phenomenex or your local Phenomenex distributor for additional GC products and applications.
Phenomenex
105
GC Accessories
Injection Port Inlet Liners (contd)
Liners for Thermo Scientific GC Systems
Liners
Inlet Liners | GC COLUMNS & ACCESSORIES
Description
Thermo Scientific Systems
Benefits / Uses
Dimensions
ID x L x OD
(mm)
Units
TRACE /
FOCUS
5 x 105 x 8.0
092046
09204625
GC Model No.
Part No.
AG0-4679
AG0-7863
Unit
Split / Splitless Double Taper FocusLiner

with wool
Trace analysis of dirty

samples
Splitless Liner
General use
Mega 4000 /
5000, & 6000
3 x 79.5 x 5.0
5/pk
25/pk
45300400
AG0-4643
5/pk
Splitless Liner
General use
TRACE /
FOCUS
3 x 105 x 8.0
5/pk
25/pk
AG0-4645
5/pk
Split / Splitless / Single Taper Liner / Trace
Trace analysis
TRACE /
FOCUS
5 x 105 x 8.0
TRACE /
FOCUS
5 x 105 x 8.0
ea
5/pk
25/pk
5/pk
AG0-7852
5/pk
092150
AG0-8669
5/pk
45350033
20945
20946
20947
Price
5/pk
25/pk
General use
Splitless, Single Taper Liner

analysis
Mega 4000 /
5000, & 6000
3 x 79.5 x 5.0
5/pk
092041
AG0-8670
5/pk
Splitless, FocusLiner

samples
TRACE /
FOCUS
5 x 105 x 8.0
5/pk
092045
AG0-8671
5/pk
Splitless Single Taper Liner

analysis
TRACE /
FOCUS
3 x 105 x 8.0
5/pk
092141
AG0-8672
5/pk
Split FocusLiner with wool

samples
TRACE /
FOCUS
5 x 105 x 8.0
5/pk
092048
AG0-8673
5/pk
Liners for Varian GC Systems

Liners
Varian
GC Model No.
Dimensions
ID x L x OD
(mm)
Units
4 x 72 x 6.3
092025
Part No.
AG0-4684
Unit
1075/1077
1078/1079
3.4 x 54 x 5
092036
AG0-4685
5/pk

analysis
1075/1077
2 x 75 x 6.3
ea
5/pk
25/pk
not deactivated
01-900109-05
03-949437-09
20721
20722
20723
AG0-4671

analysis
1075/1077
SPI (septum-equipped
programmable injector) Liner (0.25 mm)
Great linearity for 0.25

& 0.32 mm IDs
1093/1094
Great linearity for 0.53

mm ID
1093/1094
16-000830-00
01-900109-05
01-900109-06
03-918332-01
01-900109-07
20904
20905
20906
20775
20776
20777
SPI (septum-equipped
programmable injector) Liner (0.50 mm)
ea
5/pk
25/pk
ea
5/pk
25/pk
not deactivated
ea
5/pk
25/pk
AG0-4673
AG0-4675
AG0-4677
Straight Liner
Trace analysis
1078/1079
0.5 x 54 x 5.0
5/pk
092031
AG0-8665
5/pk
Split / Splitless FocusLiner

samples
1078/1079
3.4 x 54 x 5.0
5/pk
092037
AG0-8666
5/pk
Split / Splitless, Single Taper Liner

analysis
1078/1079
3.4 x 54 x 5.0
5/pk
092038
AG0-8667
5/pk
Splitless, Single Taper Liner

analysis
1078/1079
2 x 54 x 5.0
5/pk
092039
AG0-8668
5/pk
Description
Benefits / Uses
Split / Splitless Single Taper / Gooseneck Tapered Great recovery and

linearity for trace
analysis of active
compounds
Split / Splitless Double Taper / Gooseneck Tapered General use and large
injections
Splitless Liner
Split / Splitless Liner
4 x 75 x 6.3
0.5 x 54 x 4.6
0.8 x 54 x 4.6
20778
20779
20780
Price
5/pk
5/pk
5/pk
5/pk
5/pk
106
Phenomenex
GC Accessories
Sealing O-Rings
Part No.
Description
Sealing Rings for Agilent Inlet Liners
AG0-7326
Sealing ring, Viton Fluorocarbon, rated to < 300C
AG0-7327
Sealing ring, graphite, rated to 450C
Sealing Rings for PerkinElmer Inlet Liners
AG0-8674
Sealing ring for 6.2 mm OD inlet liners
Sealing Rings for Shimadzu Model 2010 Inlet Liners
AG0-8675
Sealing ring, Viton
Thermally stable
Graphite suitable for GC/MS
Septa
Unit
5180-4182
5180-4168
10/pk
10/pk
N9302783
10/pk
036-11203-84
10/pk
Price
* Similiar to but not always an exact equivalent to the original manufacturers product.
Advanced silicone formulations reduce coring, enhance durability and

re-sealing capabilities, and increase septum lifetime. GuideRight
version guides the needle during injection for longer lifetime and less
bent needles.
PhenoGrey-250
General purpose silicone rubber septum for use up to 250C
Septa Sizes by GC Instrument

Manufacturer
Instrument Model
Septa Diameter
Agilent (HP)
PhenoBlue-300
Antek
Thermo Scientific
Finnigan
GOW-MAC
HNU
PerkinElmer
Shimadzu
Tracor
5850, 5880, 5890, 6850, 6890, 7890

5700 series, 5880A
All
7/16 in. (11 mm)

3/8 in. (9.5 mm)
3/8 in. (9.5 mm)
Low-bleed septum heat stable to 350C

Durometer rating of 50-60 for guaranteed 100 injections
minimum at 300C
PhenoRed-400
Low-bleed red septum for use up to 400C
Varian
Designed and conditioned for high sensitivity analysis

Durometer rating of 50 with typical injection life of 150
punctures
Available with or without needle GuideRight Hole
PhenoGreen -400
Long life, high-temperature green septum for use up to 400C

Available with or without needle GuideRight Hole
Silicone Rubber Septa
Part No.
Description
PhenoGrey 3/8 in. (9.5 mm) Diameter
AG0-4686
PhenoGrey-250 Injector Septa
AG0-4687
PhenoBlue 3/8 in. (9.5 mm) Diameter
AG0-4688
PhenoBlue-300 Injector Septa
AG0-4689
PhenoRed 3/8 in. (9.5 mm) Diameter
AG0-4690
PhenoRed-400 Injector Septa
AG0-4691
PhenoGreen 3/8 in. (9.5 mm) Diameter
AG0-8572
PhenoGreen-400 Injector Septa
Injector Septa Plug

High temperature silicone
Phenomenex
Unit
50/pk
100/pk
50/pk
100/pk
50/pk
100/pk
50/pk
Price
9600
All
Portable GC
Sigma series, 900, 9900, 8000, Autosampler
All
540
550, 560
Packed column injectors
SPI
3700 / Vista capillary injectors
Saturn GC/MS
GuideRight Injection Hole Septa
Part No.
Description
PhenoGreen 3/8 in. (9.5 mm) Diameter 400C
AG0-7874
PhenoGreen 400 Injector Septa
PhenoRed 3/8 in. (9.5 mm) Diameter 400C
AG0-7916
PhenoRed 400 Injector Septa
PhenoGreen 7/16 in. (11 mm) Diameter 400C
AG0-7875
PhenoGreen 400 Injector Septa
PhenoRed 7/16 in. (11 mm) Diameter 400C
AG0-7917
PhenoRed 400 Injector Septa
Part No.
Description
PhenoGrey 7/16 in. (11 mm) Diameter
AG0-4692
AG0-4693
PhenoBlue 7/16 in. (11 mm) Diameter
AG0-4694
AG0-4695
PhenoRed 7/16 in. (11 mm) Diameter
AG0-4696
AG0-4697
PhenoGreen 7/16 in. (11 mm) Diameter
AG0-8573
PhenoGreen-400 Injector Septa
Shimadzu Injector Septa Plug
Part No.
Description
AG0-7517
Septa Plug for Shimadzu Injectors
(9A, 14, 15A, 16, 17A, 2010) Rated to 350 C
AG0-8802
Septa BTO Plugs, Rated to 400 C
3/8 in. (9.5 mm)

3/8 in. (9.5 mm)
3/8 in. (9.5 mm)
7/16 in. (11 mm)
plug
11/24 in. (11.5 mm)
3/8 in. (9.5 mm)
3/8 in. (9.5 mm)
7/16 in. (11 mm)
7/16 in. (11 mm) or
11/24 in. (11.5 mm)
Unit
Price
50/pk
50/pk
50/pk
50/pk
Unit
GC COLUMNS & ACCESSORIES | Sealing o-rings / septa
Durometer rating of 40-45 for easy septum puncture for up to

100 injections
Price
50/pk
100/pk
50/pk
100/pk
50/pk
100/pk
50/pk
Unit
50/pk
Price
50/pk
107
GC Accessories
Syringes
Needle Gauge Dimensions
Gauge
26
26s
25
23s
23
22s
22
OD
0.018 in. 0.46 mm
0.019 in. 0.47 mm
0.021 in. 0.51 mm
0.025 in. 0.64 mm
0.025 in. 0.64 mm
0.028 in. 0.72 mm
0.028 in. 0.72 mm
0.0102 in.
0.0050 in.
0.0102 in.
0.0060 in.
0.0132 in.
0.0060 in.
0.0162 in.
ID
0.26 mm
0.13 mm
0.26 mm
0.15 mm
0.34 mm
0.15 mm
0.41 mm
Needle Point Styles

Style
Description
Advantage
AS/Cone
Exclusively for Agilent autosamplers
2, BV (Beveled tip)
Optimal septum penetration for reduced septum coring
3, LD (Blunt tip)
General sample pipetting and HPLC injectors
5 (Conical with side hole)
Side hole for sample filling and dispensing
H (Dome with side hole)
Side hole for sample filling and dispensing. Dome tip minimizes septum coring.
SYRINGES | GC COLUMNS & ACCESSORIES
Needle / Needle Connection Styles

Style
ASN
RN
N
KH
LTN
TLL
F
R
FLL
LL
Description
Autosampler Needle
Removable Needle
Cemented Needle
Knurled Hub
Luer Tip Cemented Needle
PTFE Luer Lock
Fixed Needle
Removable Needle
Fixed Luer Lock
Luer Lock
Manufacturer
Hamilton
Hamilton
Hamilton
Hamilton
Hamilton
Hamilton
SGE
SGE
SGE
SGE
Advantage
For use with autosamplers
Allows freedom to change needle style or replace broken needles
Low dead volume
Able to handle pressure up to 6000 psig
Fixed needle with Luer Tip
Luer Lock with locking hub for use with syringe filters
Economical and more reproducible
Allows freedom to change needle style or replace broken needles
Allows use with syringe filters
Allows use with syringe filters
Autosampler Syringes
Syringes for Agilent GC 7673, 7683, and 6850 Autosampler Systems
Hamilton Syringes
Volume
Needle/Needle
(L)
Connection
Microliter Syringes
5
ASRN
5
ASN
5
ASN
5
ASRN
5
ASN
5
ASRN
5
ASN
10
ASRN
10
ASN
10
ASN
10
ASRN
10
ASN
10
ASN
10
ASRN
10
ASN
Teflon Tip Gastight Syringes
5
ASN
10
ASN
Gauge
Needle
Length
Needle
Style
Model
Mfr. No
Similar to
Mfr. No.*
23s
23s
23s
26s
26s
23s-26s
23s-26s
23s
23s
23s
23s
26s
23s-26s
23s-26s
23s-26s
1.71 in.
1.71 in.
1.71 in.
1.71 in.
1.71 in.
1.71 in.
1.71 in.
1.71 in.
1.71 in.
1.71 in.
1.71 in.
1.71 in.
1.71 in.
1.71 in.
1.71 in.
Agilent
Agilent
Agilent
Agilent
Agilent
Agilent
Agilent
Agilent
Agilent
Agilent
Agilent
Agilent
Agilent
Agilent
Agilent
75
75
75
75
75
75
75
701
701
701
701
701
701
701
701
87957
87987
87990
87958
87989
87959
87993
80357
80387
80390
80358
80389
80393
80359
80391
24793
9301-0892
20170
24792
21230
21211
24593
24795
9301-0713
9301-0725
24794
24599
24596
21213
24600
23s
23s-26s
1.71 in.
1.71 in.
Agilent
Agilent
175
701
80074
80079
24893
n/a
20168
20167
20169
Unit
Part No.
ea
ea
6/pk
ea
6/pk
ea
ea
ea
ea
6/pk
ea
6/pk
ea
ea
6/pk
AS0-8834
AS0-4386
AS0-7641
AS0-8833
AS0-8683
AS0-8831
AS0-8380
AS0-8836
AS0-4387
AS0-4388
AS0-8835
AS0-4389
AS0-8684
AS0-8832
AS0-8685
ea
ea
AS0-8686
AS0-8837
Price
108
Phenomenex
GC Accessories
Autosampler Syringes (contd)
Syringes for Agilent GC 7673, 7683, and 6850 Autosampler Systems (contd)
Gauge
Needle
Length
Needle
Style
Model
Mfr. No
Similar to
Mfr. No.*
23
23
23
26
26
26
23s-26s
23-26
23-26
26
23
23
26
26
23
23s-26s
23s-26s
23-26
42 mm
42 mm
42 mm
42 mm
42 mm
42 mm
42 mm
42 mm
42 mm
42 mm
42 mm
42 mm
42 mm
42 mm
42 mm
42 mm
42 mm
42 mm
Cone
Cone
Cone
Cone
Cone
Cone
Cone
Cone
Cone
Cone
Cone
Cone
Cone
Cone
Cone
Cone
Cone
Cone
5F-HP-0.63C
SK-5F-HP-0.63
5R-HP-0.63C
5F-HP-0.47C
SK-5F-HP-0.47
5R-HP-0.47C
SK-5F-HP-0.63/0.47
5F-HP-0.63*0.47
5R-HP-0.63/0.47C
SK-10F-HP-47
10RHP-0.63
10F-HP-0.63
SK-10F-HP-0.47
10R-HP-0.47C
SK-10F-HP-0.63
10F-HP-0.63/0.47
SK-10F-HP-0.63/0.47
10R-HP-0.63/0.47C
001810
001814
001815
001800
001804
001805
001822
001821
001825
002804
002815
002810
002804
002805
002814
002821
002822
002825
24781
24783
24793
24780
24782
24792
21214
5181-1273
21211
24786
24795
24785
24786
24794
24787
21212
21215
21213
23
23-26
23-26
23-26
42 mm
42 mm
42 mm
42 mm
Cone
Cone
Cone
Cone
HF-HP-GT-0.63
10R-HP-GT-0.63/0.47
10F-HP-GT-0.63/0.47
SK10F-HP-FT-0.63/0.47
002812
002829
002826
002827
24789
21220
5181-1267
5181-3361
21210
Unit
Part No.
ea
6/pk
ea
ea
6/pk
ea
6/pk
ea
ea
6/pk
ea
ea
6/pk
ea
6/pk
ea
6/pk
ea
AS0-8839
AS0-8687
AS0-8843
AS0-8838
AS0-8688
AS0-8842
AS0-8689
AS0-5208
AS0-8840
AS0-7637
AS0-8690
AS0-8691
AS0-7637
AS0-8844
AS0-8692
AS0-8693
AS0-8694
AS0-8841
ea
ea
ea
6/pk
AS0-8695
AS0-8696
AS0-5209
AS0-5210
Price
Syringes for PerkinElmer AutoSystem and Clarus 500 GC Systems

SGE Syringes
Volume
Needle/Needle
(L)
Connection
Microliter Syringes
0.5
R
5
F
Gauge
Needle
Length
Needle
Style
Model
Mfr. No
Similar to
Mfr. No.*
Unit
Part No.
23
23
70 mm
70 mm
Cone
Cone
0.5BR-PE-0.63
5F-PE-0.63
000478
001954
24811
24813
ea
ea
AS0-8697
AS0-8698
Price
Syringes for Varian 8000 Series GC Systems

SGE Syringes
Volume
Needle/Needle
(L)
Connection
Microliter Syringes
10
R
10
F
10
F
Gauge
Needle
Length
Needle
Style
Model
Mfr. No
Similar to
Mfr. No.*
Unit
Part No.
25
26
26
53 mm
50 mm
50 mm
Side Hole
Cone
Bevel
10R-VA8X-II
10F-C/F-5/0.47C
10F-VA8400-5/0.47
002924
002980
002950
24852
24922
21202
ea
ea
ea
AS0-8699
AS0-8700
AS0-8701
GC COLUMNS & ACCESSORIES | SYRINGES
SGE Syringes
Volume
Needle/Needle
(L)
Connection
Microliter Syringes
5
F
5
F
5
R
5
F
5
F
5
R
5
F
5
F
5
R
10
F
10
R
10
F
10
F
10
R
10
F
10
F
10
F
10
R
10
F
10
R
10
F
10
F
Price
Syringes for Shimadzu AOC 9GC Systems

Hamilton Syringes
Volume
Needle/Needle
(L)
Connection
Microliter Syringes
5
RN
5
N
10
RN
SGE Syringes
Volume
Needle/Needle
(L)
Connection
Microliter Syringes
10
R
Gauge
Needle
Length
Needle
Style
Model
Mfr. No
Similar to
Mfr. No.*
Unit
Part No.
26s
26s
26s
2 in. /51 mm
2 in. /51 mm
2 in. /51 mm
2
2
2
75RN
75N
701RN
87930
87900
80330
24617
24938
24530
ea
ea
ea
AS0-8702
AS0-4390
AS0-0100
Gauge
Needle
Length
Needle
Style
Model
Mfr. No
Similar to
Mfr. No.*
Unit
Part No.
23
42 mm
Cone
10R-S-0.63
002898
24845
ea
AS0-8703
Price
Price
*Similar to but not always an exact equivalent to the original manufacturer's product.
Phenomenex
109
GC Accessories
Autosampler Syringes (contd)
Syringes for Thermo Scientific GC Systems
SGE Syringes
Volume
Needle/Needle
(L)
Connection
Microliter Syringes
10
F
10
R
Gauge
Needle
Length
Needle
Style
Model
Mfr. No
Similar to
Mfr. No.*
Unit
Part No.
26
26
80 mm
80 mm
Cone
Cone
10F-C/F-8/0.47C
10R-C/F-8/0-0.47C
002992
002993
24924
24934
ea
ea
AS0-8704
AS0-8705
Price
Syringes for CTC/Leap GC Systems
SYRINGES | GC COLUMNS & ACCESSORIES
Hamilton Syringes
Volume
Needle/Needle
(L)
Connection
Microliter Syringes
1.2
N
10
N
25
N
110
SGE Syringes
Volume
Needle/Needle
(L)
Connection
Microliter Syringes
10
F
10
R
10
F
10
F
10
R
10
F
Gauge
Needle
Length
Needle
Style
Model
Mfr. No
Similar to
Mfr. No.*
Unit
Part No.
26
26s
22s
2 in./51 mm
2 in./51 mm
2 in./51 mm
Cone
2
3
7701.2N
701N
1702N
203185
203269
203075
22755
22762
ea
6/pk
ea
AS0-8706
AS0-8707
AS0-8708
Gauge
Needle
Length
Needle
Style
Model
Mfr. No
Similar to
Mfr. No.*
Unit
Part No.
23
23
26
26
26
50 mm
50 mm
50 mm
50 mm
50 mm
Cone
Cone
Cone
Cone
Cone
10F-C/F-5/0.63
10R-C/F-5/0.63
10F-C/F-5/0.47C
SK10F-C/F-5/0.47C
10R-C/F-5/0.47C
002981
002984
002980
002986
002982
24923
24932
24922
24925
24930
ea
ea
ea
6/pk
ea
AS0-7638
AS0-8709
AS0-8700
AS0-8711
AS0-8712
23
50 mm
Cone
10F-C/T-GT-5/0.63C
002987
22749
ea
AS0-8713
Price

Manual Syringes
Hamilton Syringes
Volume
Needle/Needle
(L)
Connection
Microvolume Syringes
5
N
5
RN
10
N
10
N
10
RN
25
N
10
N
Positive Displacement Syringes
0.5
KH
1.0
KH
1.0
KH
1.0
KH
2.0
KH
5.0
KH
10
N
10
RN
25
N
25
RN
50
N
50
RN
100
N
100
RN
250
N
Price
Gauge
Needle
Length
Needle
Style
Model
Mfr. No
Similar to
Mfr. No.*
Unit
Part No.
26s
26s
26s
26s
26s
22s
26s
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
2
2
2
2
2
2
5
75N
75RN
701N
701N
701RN
702N
701NPT5
87900
87930
80300
80366
80330
80400
80339
24938
24617
20174
20175
24530
24531
24967
ea
ea
ea
6/pk
ea
ea
ea
AS0-4390
AS0-8702
AS0-0077
AS0-0016
AS0-0100
AS0-0078
AS0-0094
25
22
25s
25s
25
24
2.75 in./70 mm
2.75 in./70 mm
2.75 in./70 mm
2.75 in./70 mm
2.75 in./70 mm
2.75 in./70 mm
2
2
2
3
2
2
7000.5
7101
7001
7001
7002
7105
86259
86211
80135
80100
88411
88011
24545
24549
24547
24548
24551
24555
ea
ea
ea
ea
ea
ea
AS0-8715
AS0-8716
AS0-1918
AS0-8717
AS0-8718
AS0-8719
26s
26s
22s
22s
22s
22s
22s
22s
22s
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
2
2
2
2
2
2
2
2
2
1701
1701
1702
1702
1705
1705
1710
1710
1725
80000
80030
80200
80230
80900
80930
81000
81030
81100
24557
24558
24559
24560
24561
24562
24563
24564
24567
ea
ea
ea
ea
ea
ea
ea
ea
ea
AS0-4391
AS0-1898
AS0-8720
AS0-1899
AS0-0061
AS0-1900
AS0-0062
AS0-1901
AS0-0063
Price
Phenomenex
GC Accessories
Manual Syringes (contd)
Hamilton Syringes (continued)
Volume
Needle/Needle
(L)
Connection
Gauge
Teflon Tip Gastight Syringes (continued)
250
RN
22s
500
LTN
22
500
RN
22
1 mL
LTN
22
1 mL
RN
22
1 mL
TLL
2.5 mL
TLL
2.5 mL
RN
22
2.5 mL
N
22
5 mL
TLL
10 mL
TLL
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
2 in./51 mm
Needle
Style
Model
Mfr. No
Similar to
Mfr. No.*
Unit
Part No.
2
2
2
2
2
w/o slots
w/o slots
2
2
w/o slots
w/o slots
1725
1750
1750
1001
1001
1001
1002
1002
1002
1005
1010
81130
81217
81230
81317
81330
81320
81420
81430
81417
81520
81620
24568
24571
24572
24575
24576
24578
24584
24582
24581
20178
20179
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
AS0-1902
AS0-0064
AS0-1903
AS0-0065
AS0-8721
AS0-1907
AS0-1908
AS0-8722
AS0-8723
AS0-8724
AS0-1910
Gauge
Needle
Length
Needle
Style
Model
Mfr. No
Similar to
Mfr. No.*
Unit
Part No.
26
26
26
26
26
26
50 mm
50 mm
50 mm
50 mm
50 mm
50 mm
2
2
2
2
2
2
5F
5R
10F
SK-10F
10R
SK-10R
001000
001050
002000
002030
002050
002080
24700
24701
24702
24715
24703
24716
ea
ea
ea
6/pk
ea
6/pk
AS0-8725
AS0-8726
AS0-8727
AS0-8728
AS0-8729
AS0-8730
23
23
26
23
70 mm
70 mm
70 mm
70 mm
2
2
Cone
2
0.5BR-7BV
1BR-7BV
1BR-7/0.47
5BR-7BV
000311
000506
000570
000803
24771
24775
24776
24779
ea
ea
ea
ea
AS0-8737
AS0-8738
AS0-8739
AS0-8740
26
26
25
25
25
25
25
25
25
25
25
23
23
50 mm
50 mm
50 mm
50 mm
50 mm
50 mm
50 mm
50 mm
50 mm
50 mm
50 mm
50 mm
50 mm
2
2
2
2
2
2
2
2
2
2
2
Bevel
Bevel
10F-GT
10R-GT
25F-GT
50F-GT
50R-GT
100F-GT
100R-GT
250F-GT
250R-GT
500F-GT
500R-GT
1MDR-GT
1MDF-LL-GT
MDR-GT
5MDR-LL-GT
5MDF-LL-GT
10MDR-LL-GT
10MDR-LL-GT
25MDR-LL-GT
25MDR-LL-GT
002200
002250
003200
004200
004250
005200
005250
006200
006250
007200
007250
008100
008025
008500
008760
008762
008960
008962
009462
009463
24725
24726
24727
24729
24730
24734
24735
24738
24739
24742
24743
24750
24752
24757
24759
24760
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
AS0-8741
AS0-8742
AS0-8743
AS0-8744
AS0-8745
AS0-8746
AS0-8747
AS0-8748
AS0-8749
AS0-8750
AS0-8751
AS0-8845
AS0-0120
AS0-8752
AS0-0121
AS0-8753
AS0-0122
AS0-8754
AS0-8755
AS0-8756
Price
Price
GC COLUMNS & ACCESSORIES | SYRINGES
SGE Syringes
Volume
Needle/Needle
(L)
Connection
Microvolume Syringes
5
F
5
R
10
F
10
F
10
R
10
R
Positive Displacement Syringes
0.5
R
1.0
R
1.0
R
5.0
R
10
F
10
R
25
F
50
F
50
R
100
F
100
R
250
F
250
R
500
F
500
R
1 mL
RN
1 mL
LL
2.5 mL
R
5 mL
LL
5 mL
FLL
10 mL
LL
10 mL
FLL
25 mL
LL
25 mL
FLL
Needle
Length

Phenomenex
111
GC Accessories
Moisture, Oxygen, and Hydrocarbon
Gas Traps/Purifiers
Extends column lifetimes
Protects columns from irreversible damage
Improve analytical reliability (identification and quantitation results)
Reliable and affordable high-capacity, high-performance purifiers
Gas traps/purifiers are recommended, even with ultra-high purity
gases. Trace contaminants can cause unstable baselines, high detector noise, ghost peaks and even column damage. Oxygen is
especially detrimental to more-polar columns and can cause oxidation of the stationary phase, column bleed, loss of retention as well
as reduced column lifetimes.
The types of gas traps that are recommended depend on the type
and use of the gas. A summary of common gases, their uses, and
the traps that are recommended are below. If multiple traps are recommended, they are to be installed in the order listed.
Recommended Gas Traps

Use
Carrier Gas
FID, NPD
gas purifiers / oxygen / moisture traps | GC COLUMNS & ACCESSORIES
ECD
TCD
Gas
Recommended Trap(s)
Helium, Hydrogen, or Nitrogen

Air
Make-up
Hydrogen
Make-up
Same as carrier
Moisture, Hydrocarbon, Oxygen

Hydrocarbon
Hydrocarbon
Hydrocarbon
Water, Oxygen
Moisture, Hydrocarbon, Oxygen
Moisture, Oxygen, and Hydrocarbon Gas Traps / Purifiers
Type
Media
Max Pressure
Moisture
Molecular Sieve 13x
Hydrocarbon
Impregnated
carbon filter media
100 psi
250 psi
50 psi
Oxygen
Purity
Capacity
100 cc
Indicating
Yes
10 ppb water
250 cc
Yes
250 cc
Yes
100 cc
No
100 cc
No
200 cc
No
200 cc
No
50 cc
Yes
Call for specific

compounds
1 ppb oxygen
Proprietary
Oxygen / Moisture
Proprietary
250 psi
5 ppb oxygen
250 psi
15 ppb oxygen
and water
Fittings
1
/8 in.
Part No.
AG0-4766
Unit
ea
/8 in.
AG0-4768
ea
/4 in.
AG0-4769
ea
/8 in.
AG0-4770
ea
/4 in.
AG0-4771
ea
/8 in.
AG0-4772
ea
/4 in.
AG0-4773
ea
/8 in.
AG0-4774
ea
/4 in.
AG0-4775
ea
/8 in.
AG0-4776
ea
/4 in.
AG0-4777
ea
50 cc
Yes
150 cc
Yes
150 cc
Yes
5.5 x 2 in.
No
5.5 x 2 in.
No
150 cc
No
150 cc
No
/8 in.
AG0-4792
ea
/4 in.
AG0-4791
ea
/8 in.
AG0-4778
ea
/4 in.
AG0-4779
ea
Price
To get the greatest lifetime out of gas traps, try placing a large capacity non-indicating trap in-line before an indicating trap. Replace the
large capacity trap only when the indicating trap starts to change color. Mark the color transition on the indicating trap with a marker. The
color transition moves only when the large capacity trap is saturated. This gives long term savings by eliminating unnecessary maintenance
and maintains quality gas.

112
For maximum efficiency, flow rates for any trap should not
exceed 3 L/min. Trapping efficiency will drop-off rapidly as
flow rates increase.
Phenomenex
GC Accessories
Merlin Microseal Septum
Improves chromatographic performance and quantitative
results by reducing bleed and ghost peaks
Improves reliability and performance during extended
automated runs
Thousands of injections before seal replacement
Save time changing septa, instrument downtime and
troubleshooting
The Merlin Microseal Septum is a long-life replacement for the
standard septum on the capillary inlet or the purged-packed inlet
of Agilent GC systems.
The Microseal is a patented inlet assembly that provides two distinct sealing mechanisms. The first is a double O-ring seal around
the syringe that ensures gas leak integrity during time of injection.
There is no traditional septum to core or flake, which can cause
bleeding and ghost peaks, and less force is required for the user
to make a manual injection. The second seal is a spring-assisted
duckbill that reliably maintains a high-pressure seal within the
injection port at all times.
O-ring seals needle during injection
Since the syringe does not pierce any septum material, seal lifetime is significantly extended Microseals are typically used for
up to one year or more under normal conditions before septum
replacement. Many laboratories experience lifetimes of 5,000 to
10,000 injections or more. This means you save tremendously
in time changing septa, instrument downtime and troubleshooting. Chromatographic performance will also improve due to less
bleed and fewer ghost peaks, improving quantitation and data
reliability.
Spring
(1) Complete High Pressure Kits include the nut and one or
two Microseal septa. All High Pressure Kits are rated for inlet
pressures up to 100 psi. For long-term operation (>6 months)
with the Microseal septum use the following injection port
limits: (a) Agilent 6890, 5890 Series II - 325C; (b) Agilent 5890A
- 300C. Higher temperatures will result in shorter lifetimes.
(2) The Merlin Microseal Septum should only be used with a
blunt-tipped, 0.63 mm diameter (0.025 in., 23 gauge) syringe
needle (typically used with the Agilent 7673 autosampler).
Sharp-pointed or sharp-edged needles should not be used as
they may slice or pierce the seals.
Instrument Compatibility: The Merlin Microseal systems can

be used manually with all Agilent 5800, 6800, and 7890 series
GCs. However, for autosampler use it is compatible with only
the 7673A and 7673B units. Other inlets or autosampler systems
from Agilent or other manufacturers are not compatible.
Merlin Microseal Septum
Part No.
Description
AG0-5985
Merlin Microseal High Pressure Septum Standard Kit, includes nut and 2 septa
AG0-5986
Merlin Microseal High Pressure Septum Starter Kit, includes nut and 1 septum
Replacement Parts
AG0-5987
Merlin Microseal High Pressure Septum
AG0-5988
Merlin Microseal High Pressure Nut
Unit
ea
ea
Price
GC COLUMNS & ACCESSORIES | MERLIN MICROSEAL SEPTUM
Duckbill opens easily, seals reliably
ea
ea
Syringes for Use With the Merlin Microseal

Part No.
Mfr. No.
Agilent P/N
For Agilent 7673 Autosamplers
AS0-4386
87987
9301-0892
AS0-4387
80387
9301-0713
AS0-4388
80390
9301-0725
Removable Needles
AS0-4401
7786-01
9301-0892
Description*
75ASN (23s/1.71in./HP)
701ASN (23s/1.71in./HP)
701ASN (23s/1.71in./HP)
RN (23s/1.71in./HP)
Capacity (L)
Unit
5
10
10
ea
ea
6/pk
2.5-100 L
6/pk
Price
NOTE: Replacement needles are available in packs of three.

* Values in parentheses denote the following: (gauge/length/point style). s after gauge denotes smaller/reduced internal needle diameter.
Phenomenex
113
GC Accessories
Ferrule Remover
Tool Kit
Ceramic Scoring Wafers

High quality ceramic cutting tool for fused silica
capillary columns
Simple and convenient tools effectively remove

stuck ferrules from fittings
Special spiral-cut rachet design slides in and
grabs ferrule tight
Includes two tools for removing capillary ferrules
from 0.4 to 0.8 mm ID
Ceramic Scoring Wafers / Tool & Cleaning Kits | GC COLUMNS & ACCESSORIES
Ferrule Remover Tool Kit
Part No.
Description
AD0-4725
GC Ferrule Remover Tool Kit
Unit
ea
Price
Ceramic Scoring Wafers
Part No.
Description
AG0-4718
Ceramic Scoring Wafer
Unit
2/pk
Flame Detector Jet

Cleaning Kit
Injection Port
Cleaning Kit
For routine maintenance of Flame Ionization Detectors
Includes 4 stainless steel brush sizes 1/8, 3/16,

1/4 and 3/8 in.
Includes:
Also includes a scraper-pick combo for hard

septum residues and septum removal
-- Set of 3 jet reamers 0.008, 0.08 and 0.02 in.

-- 1 Stainless steel and 1 brass brush
-- 1 Dual-ended pin vise for holding reamers and brushes
Easy to use with twisted wire handles and a

convenient wire ring to keep parts together
Use after the flame jet has been taken apart or while
installed in the GC
Flame Detector Jet Cleaning Kit
Part No.
Description
AD0-4723
GC Flame Detector Jet Cleaning Kit
Price
Unit
ea
Price
Injection Port Cleaning Kit
Part No.
Description
AD0-4724
GC Injection Port Cleaning Kit
Unit
ea
Price
114
Phenomenex
Zebron GC Column Applications
environmental
Semi-volatile Organics:
EPA Method 8270C
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
App ID 14945
Zebron ZB-5 with 5 meter Guardian

30 meter x 0.25 mm x 0.25 m
7HG-G002-11-GGA
Split 10:1 @ 250 C, 1 L
50 C for 2 min to 70 C @ 20 C/min to
195 C @ 16 C/min to 285 C @ 30 C/min to
Detector: MSD; 30-450 amu
11
13
15
17
19
21 min
18-20
69,70
56,57
40,41 4243
8.5
44 45
51
54,55
46 48
53
47 49 50
52
9.5
10.5
84,85
103,104
83
74
59
9 min
80
66,67
77,78 79
68
75
82
63
81
72
64
71
65
73
58 61
76
60
62
11.5
12.5 min
90 92
86
89
87
88
13
14
96 98
97
91 95
94
93
102
101
100
99
15
107
106
105
110,111
108
112
109
16
17
18
19
20
21 min
gc columns & accessories
1 2
36,37
21-22
35
34
29
23
13
39
15
12
16
27 313233
14
38
9 11
17 2425 26 28
8 10
30
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
Phenomenex
N-Nitrosodimethylamine
Pyridine
2-Picoline
N-Nitrosomethylethylamine
Methyl Methanesulfonate
N-Nitrosodiethylamine
Ethyl Methanesulfonate
Phenol
Aniline
Bis(2-chloroethyl)ether
2-Chlorophenol
1,3-Dichlorobenzene
1,4-Dichlorobenzene
Benzyl Alcohol
1,2-Dichlorobenzene
2-Methylphenol
Bis(chloroisopropyl)ether
4-Methylphenol; 3-Methylphenol
N-Nitrosopyrrolidine
Acetophenone
N-Nitrosomorpholine
N-Nitroso-di-n-propylamine
2-Toluidine
Hexachloroethane
Nitrobenzene
N-Nitrosopiperidine
Isophorone
2-Nitrophenol
2,4-Dimethylphenol
Peak No. Sample Analyte

30.
31.
32.
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.
50.
51.
52.
53.
54.
55.
56.
57.
58.
Benzoic Acid
Bis(2-chloroethoxy)methane
2,4-Dichlorophenol
,-Dimethylphenylamine
1,2,4-Trichlorobenzene
Naphthalene
4-Chloroaniline
2,6-Dichlorophenol
Hexachloropropene
Hexachloro-1,3-Butadiene
1,4-Phenylenediamine
N-Nitrosodibutylamine
4-Chloro-3-methylphenol
Safrole
2-Methylnaphthalene
1-Methylnaphthalene
1,2,4,5-Tetrachlorobenzene
Hexachlorocyclopentadiene
2,4,6-Trichlorophenol
Isosafrole (isomer)
2-Chloronaphthalene
2-Nitroaniline
1,4-Naphthoquinone
Dimethyl Phthalate
1,3-Dinitrobenzene
2,6-Dinitrotoluene
Acenaphthylene
3-Nitroaniline

59.
60.
61.
62.
63.
64.
65.
66.
67.
68.
69.
70.
71.
72.
73.
74.
75.
76.
77.
78.
79.
80.
81.
82.
83.
84.
85.
86.
87.
Acenaphthene
2,4-Dinitrophenol
4-Nitrophenol
Dibenzofuran
Pentachlorobenzene
2,4-Dinitrotoluene
1-Naphthalenamine
2-Naphthalenamine
2,3,4,6-Tetrachlorophenol
Diethyl Phthalate
Fluorene
4-Chlorophenyl phenyl ether
2-Methyl-5-nitroaniline
4-Nitroaniline
2-Methyl-4,6-dinitrophenol
Diphenylamine
Azobenzene
1,3,5-Trinitrobenzene
Phenacetin
4-Bromophenyl phenyl ether
Hexachlorobenzene
4-Aminobiphenyl
Pentachlorophenol
Pentachloronitrobenzene
Phenanthrene
Dinoseb
Anthracene
Di-n-butyl Phthalate
4-Nitroquinoline-n-oxide

88.
89.
90.
91.
92.
93.
94.
95.
96.
97.
98.
99.
100.
101.
102.
103.
104.
105.
106.
107.
108.
109.
110.
111.
112.
Methapyrilene
Isodrin
Fluoranthene
Benzidine
Pyrene
Aramite (isomer)
Aramite (isomer)
Dimethylaminoazobenzene
Chlorobenzilate
3,3-Dimethylbenzidine
Benzyl butyl Phthalate
Kepone
2-Acetylaminofluorene
3,3'-Dichlorobenzidine
Benz[a]anthracene
Bis(2-ethylhexyl) Phthalate
Chrysene
Di-n-octyl Phthalate
Benzo[b]fluoranthene
Benzo[k]fluoranthene
Benzo[a]pyrene
3-Methylcholanthrene
Indeno[1,2,3-cd]pyrene
Dibenz[a,h]anthracene
Benzo[g,h,i]perylene
115
128
123
124
133
131
129
130 132

3
15696
15696
135
environmental15696
10
125-127
13
12
11
125-127
100-102
68
42
43,44
80-82
45
34 35,36 39,40
32
37 38
30
46
51 52
33
56 57
47
48
6
5
38
77
4
4
6
10
7
11
8
12
41
See inset

|
116
10
105
128 123
110
108
109
124
119
133
min
10
11
131
123
130 132
124
133
134
136 131
137
129
135 130
132
134
134
11
13
min
12
16
11
15
12
13
14

N-Nitrosodimethylamine
Pyridine
2-Methylpyridine
N-Methyl-N-Nitrosoethanamine
Methyl Methanesulfonate
2-Fluorophenol (surr)
N-Nitrosodiethylamine
Ethyl Methanesulfonate
Phenol-d6 (surr)
Phenol
Aniline
Bis(2-Chloroethyl)ether
2-Chlorophenol
1,3-Dichlorobenzene
1,4-Dichlorobenzene-d4 (IS)
1,4-Dichlorobenzene
Benzoyl Alcohol
1,2-Dichlorobenzene
2-Methylphenol
Bis(2-Chloroisopropyl)ether
4-Methylphenol
3-Methylphenol
N-Nitrosopyrrolidine
Acetophenone
N-Nitroso-di-n-Propylamine
N-Nitrosomorpholine
o-Toluidine
Hexachloroethane
Nitrobenzene-d5 (surr)
Nitrobenzene
N-Nitrosopiperidine
Isophorone
2-Nitrophenol
2,4-Dimethylphenol
Triethylphosphorothioate
135
136 137
15 136 16
137
min
135
114,115
114,115
14
15
16
min
114,115
58
41
100-102
100-102
68
42
43,44 68
21-25
min
129
29
15
16
26,27
26,27
17 18 19
112
100-102
80-82
116
111 113
68
80-82
34 35,36 39,4042 45
96-98
103
117
30
69
43,44 46
34 35,36 39,40 10 45 14 17 18 19
96-98
73,74
91121
85
32
37 38
92,93
11
51 52
30
69 73,74
66104
13
106
91 60
118
85
8789,90
32
37 38
79
12 46
86
92,93
16
51
28 52
66
99
64
53,54
122
110
26,27
9
33 60
50
120 94
72 7580-82
49 79
103
63
7610778 83
86 8789,90 59
28
20 50
53,54 34 35,36 39,40 64 45
96-98 123 104
94 62 99 65
33
71
77
72 4776 78 83
56 57
49
63
10
61
30 31 57 59
696773,74 105
75
95
14 17 18 19
84
38
46
91
85
32
37
65
71
77 51 52
56
92,93
11
88
47
61 62
66
31
70
108
67
95
13
84
48
60
87 112
79
55 88
12
28
10986 89,90 94 119 99
70
64
53,54
48
72 75 76 78 83
49 50
63
59
55 33
20
57
657
71
77
56
6
8
9
4
5
min
62
47
61
31
67
95
84
min
6
7
8
9
10
11
min
5
min
88
70
48
55
20
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
35.
107
116
117
121
118
120 122
123
106
99
10
14
58
42
43,44
10
14
11
13
12
9
13
See inset
29
15
29
1338
111 113
103
104
128
47-104
58
21-25
21-25
124
131
129
130 132
9-46
123
min
41
16
63
65
61 62
67
15
59
91
85
92,93
79
86 8789,90
94
72
75 76 78 83
71
77
95
84
88
70
64
55
96-98
69 73,74
128
66
60
53,54
49 50
31
4
3
61
min
58
16
114,115
29
15
Zebron ZB-5ms
20 meter x 0.18 mm x 0.32 m
7FD-G010-51
16
26,27
Splitless @ 280 C,9 1 L
10 (constant
Helium @ 1.2 mL/min
flow)
14
17 18 19
11
45 C6to 200 C @ 2012 13
C/min to 300 C28
20
@ 25 C/min for 5.5 min
Sample: All analytes are at 15 ppm, all
2
2
internal standards (IS) and 4
surrogates (surr) are 40 ppm.
15
See inset
41
14
125-127
136 137
105-123
Semi-volatile Organics:
21-25
EPA Method 8270
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
134
App ID 15696

36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.
50.
51.
52.
53.
54.
55.
56.
57.
58.
59.
60.
61.
62.
63.
64.
65.
66.
67.
68.
69.
70.
min
Benzoic Acid
Bis(2-Chloroethoxy)methane
2,4-Dichlorophenol
1,2,4-Trichlorobenzene
Phentermine
Naphthalene-d8 (IS)
Naphthalene
4-Chloroaniline
2,6-Dichlorophenol
Hexachloropropene
Hexachlorobutadiene
N-Nitrosodibutylamine
1,4-Diphenylenediamine
4-Chloro-3-methylphenol
Isosafrole
2-Methylnaphthalene
1-Methylnaphthalene
Hexachlorocyclopentadiene
1,2,4,5-Tetrachlorobenzene
Safrole (Isomer)
2-Fluorobiphenyl (surr)
Safrole
2-Chloronaphthalene
2-Nitroaniline
1,4-Naphthoquinone
Dimethyl Phthalate
1,3-Dinitrobenzene
2,6-Dinitrotoluene
Acenaphthylene
3-Nitroaniline
Acenaphthene-d10 (IS)
Acenaphthene
2,4-Dinitrophenol

71.
72.
73.
74.
75.
76.
77.
78.
79.
80.
81.
82.
83.
84.
85.
86.
87.
88.
89.
90.
91.
92.
93.
94.
95.
96.
97.
98.
99.
100.
101.
102.
103.
104.
105.
4-Nitrophenol
Pentachlorobenzene
2,4-Dinitrotoluene
Dibenzofuran
1-Naphthylamine
Tetrachlorophenol
2-Naphthylamine
Diethyl Phthalate
Zinophos (Thionazin)
Fluorene
4-Chlorophenylphenylether
2-Methyl-5-Nitroaniline
4-Nitroaniline
4,6-Dinitro-2-Methylphenol
Diphenylamine
Azobenzene
2,4,6-Tribromophenol (surr)
1,3,5-Trinitrobenzene
Diallate
Phorate
Phenacetin
4-Bromophenylphenylether
Diallate (isomer)
Hexachlorobenzene
Dimethoate
Pentachlorophenol
Pentachloronitrobenzene
4-Aminobiphenyl
Pronamide
Phenanthrene-d10 (surr)
Dinoseb
Disulfoton
Phenanthrene
Anthracene
Methyl Parathion
min
103
104
105
min
121
118
110
120 122
116
107
111 113
123
117
105
121
108
112
106
118
109
122
110
119
120
107
123
108
109
10
10
112

106.
107.
108.
109.
110.
111.
112.
113.
114.
115.
116.
117.
118.
119.
120.
121.
122.
123.
124.
125.
126.
127.
128.
129.
130.
131.
132.
133.
134.
135.
136.
137.
116
117
111 113
106
11
119
11
min
Di-n-butyl Phthalate
Ethyl Parathion
4-Nitroquinoline-n-Oxide
Methapyrilene
Isodrin
Fluoranthene
Benzidine
Pyrene
Aramite
p-Terphenyl-d14 (surr)
Aramite (isomer)
Dimethylaminoazobenzene
Chlorobenzilate
Famfur
3,3'-Dimethylbenzidine
Benzyl butyl Phthalate
Kepone
2-Acetylaminofluorene
3,3'-Dichlorobenzidine
Benz[a]anthracene
Chrysene-d12 (IS)
Bis(2-ethylhexyl) Phthalate
Chrysene
Di-n-octylphthalate
Benzo[b]fluoranthene
Benzo[k]fluoranthene
Benzo[a]pyrene
Perylene-d12 (IS)
3-Methylcholanthrene
Indeno[1,2,3-cd]pyrene
Dibenz[a,h]anthracene
Benzo[g,h,i]perylene
Phenomenex
min
environmental
Polynuclear Aromatic Hydrocarbons:
EPA Method 8100
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:

Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-5ms
10 meter x 0.10 mm x 0.10 m
7CB-G010-02
Split 20:1 @ 320 C, 1 L
100 C to 200 C @ 33 C/min to 320C @ 25 C/min
FID @ 330 C
1. Naphthalene
3. Acenaphthalene
4. Acenaphthene
5. Fluorene
14. Benzo[a]pyrene
6. Phenanthrene
7. Anthracene
8. Fluoranthene
9. Pyrene
Zebron ZB-5ms
30 meter x 0.25 mm x 0.25 m
7HG-G010-11
Split 15:1 @ 310 C, 1 L
170 C to 240 C @ 15 C/min to 275C @ 4 C/min to
Detector: FID @ 330 C
3. Acenaphthalene
4. Acenaphthene
5. Fluorene
14. Benzo[a]pyrene
6. Phenanthrene
7. Anthracene
8. Fluoranthene
9. Pyrene
6 7
5
4
5
7
8
11
10
12
13
14
16
16
15 17
13 14
12
App ID 15806
10
min
PAHs: US and EU Compounds
10
12
14
16
18
min
Polybrominated Diphenyl Ethers (PBDEs)

15 meter x 0.25 mm x 0.10 m
7EG-G015-02
On-Column @ 73 C, 0.5 L
70 C to 160 C at 25 C/min to 350 C at 10 C/min for 5 min
ECD @ 400 C
Sample was 2.5 ppm in Isooctane
1. BDE-25
11. BDE-99
2. BDE-28
12. BDE-85
3. BDE-75
13. BDE-154
4. BDE-49
14. BDE-153
5. BDE-71
15. BDE-138
6. BDE-47
16. BDE-183
7. BDE-66
17. BDE-190
8. BDE-77
18. BDE-203
9. BDE-100
19. BDE-209
10. BDE-119
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-35
30 meter x 0.25 mm x 0.25 m
7HK-G003-11
On-Column @ 83 C, 1 L
80 C for 0.66 min to 250 C @ 20 C/min to 360 C @ 8 C/min for 6 min
MSD @ 360 C; 45-450 amu
All analytes are 10 ppm in dichloromethane
1. Naphthalene
14. Benzo[j]fluoranthene
15. Benzo[a]pyrene
3. Acenaphthylene
16. 3-Methylcholanthrene
4. Acenaphthene
17. Dibenz[a,h]acridine
5. Fluorene
18. Dibenz[a,j]acridine
6. Phenanthrene
7. Anthracene
8. Fluoranthene
9. Pyrene
22. 7H-dibenzo[c,g]carbazole
23. Dibenzo[a,e]pyrene
11. Chrysene
24. Dibenzo[a,i]pyrene
25. Dibenzo[a,h]pyrene
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
11
17
15
App ID 16017
16144
6
18525
7
19
4
3 5
14
11
910
12
13
16
15
18
21
10
15
12 13
14
11
18
17
22
Phenomenex
10
12
14
16
23
24
25
16
App ID 16144
App ID 18525
17
18
20
min
19
10
12
14
16
18
20
22 min
117
ENVIRONMENTAL
Dual Column Analysis

Zebron
MR-1
Injector
Organochlorine Pesticides - Fast:

EPA Method 8081
ECD Detector
Y-Splitter
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
5m
Zebron MultiResidue-1
Z-Guard
30 meter x 0.32 mm x 0.50 m
7HM-G016-17
Splitless @ 250 C, 1 L
100 C for 0.5 min to 220 C @ 35 C/min to 340 C @ 20 C/min
for 2 min
Detector: ECD @ 350 C
Sample: Performed using a 5 m Z-Guard Column and a 'Y' splitter.
1. TCMX (Surr)
13. 4, 4' -DDE
2. 1-Bromo-2-Nitrobenzene (IS) 14. Dieldrin
3. -BHC
15. Endrin
4. -BHC (Lindane)
16. 4, 4' -DDD
5. -BHC
17. Endosulfan II
6. -BHC
18. Endrin Aldehyde
7. Heptachlor
19. 4, 4' -DDT
8. Aldrin
21. Methoxychlor
22. Endrin Ketone
12. Endosulfan I
ECD Detector
Zebron
MR-2
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
30 meter x 0.32 mm x 0.25 m
7HM-G017-11
for 2 min
Sample: Performed using a 5 m Z-Guard Column and a 'Y' splitter.
1. TCMX (Surr)
13. 4, 4' -DDE
2. 1-Bromo-2-Nitrobenzene (IS) 14. Dieldrin
3. -BHC
15. Endrin
4. -BHC (Lindane)
16. 4, 4' -DDD
5. -BHC
17. Endosulfan II
6. Heptachlor
18. 4, 4' -DDT
7. -BHC
19. Endrin Aldehyde
8. Aldrin
21. Methoxychlor
22. Endrin Ketone
12. Endosulfan I
16154
16153
2
3
7
6
9
10
11
14
13
12
8
9 1011 14
1213
App ID 16153
22
17
1516 19 20
18
23
21
10
23
22
16 1718
15
App ID 16154

|
6 7
20
19
21
min
min

Chlorinated Pesticides: EPA Method 8081
Injector
Column: Zebron ZB-XLB-HT Inferno

Part No.: 7HG-G024-11
Zebron
XLB-HT
Column: Zebron ZB-35HT Inferno

ECD Detector
Y-Splitter
5m
Z-Guard
ECD Detector
Zebron
35HT
Conditions for both columns:

Injection: Splitless 15:1 @ 250 C,1 L
Oven Program: 160 C for 1 min to 250 C @ 15 C/min for 1 min to 320 C
@ 10 C/min
Sample: Performed using a 5 m Z-Guard Column and a Y splitter.
Analytes are 25 ppb in isooctane.
1. -BHC
8. -Chlordane
2. -BHC
9. -Chlordane
3. -BHC
10. Endosulfan I
4. -BHC
11. 4,4-DDE
5. Heptachlor
12. Dieldrin
6. Aldrin
13. Endrin
14. 4,4-DDD
15.
16.
17.
18.
19.
20.
Endosulfan II
Endrin aldehyde
4,4-DDT
Endosulfan sulfate
Methoxychlor
Endrin ketone
18786
4
2
18787
11
9
10
1
12
14
15
13
8 9 11 12
10
17 18
10
12
14
13
15
17
20
18
16
3
App ID 18786
19
118
5 4
20
16
14 min
App ID 18787
19
10
12
14
min
Phenomenex
ENVIRONMENTAL
Injector
Zebron
MR-1
ECD Detector
Organophosphorous Pesticides:
EPA Method 8141
Y-Splitter
5m
Z-Guard
ECD Detector
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
30 meter x 0.32 mm x 0.25 m
7HM-G017-11
100 C for 0.5 min to 180 C @ 20 C/min to 240 C @ 6 C/min to
Sample: Analytes are 2 ppm in Dichloromethane. Columns connected using a
5 m Z-Guard Column and a 'Y' splitter.
1. Dichlorvos
17. Dimethoate
33. Chlorfenvinphos
2. Mevinphos
18. Disulfoton
34. Crotoxyphos
3. Trichlorfon
19. Phosphamidon Isomer 35. Stirofos
4. TEPP
20. Dichlofenthion
36. Tokuthion
5. Demeton Isomer
21. Phosphamidon
37. Merphos Oxide (Tribufos)
6. Thionazin
22. Chlorpyrifos Methyl
38. Ethion
7. Ethoprop
23. Ronnel
39. Fensulfothion
8. Sulfotep
24. Aspon
40. Contaminant
9. Naled
25. Methyl Parathion
41. Carbophenothion
10. Phorate
26. Malathion
42. Famfur
11. Dicrotophos
27. Fenitrothion
43. EPN
12. Demeton
28. Chlorpyrifos
44. Phosmet
13. Monocrotophos
29. Trichloronate
45. Leptophos
14. Terbufos
30. Fenthion
46. Azinphos Methyl
15. Diazinon
31. Parathion
47. Azinphos Ethyl
16. Fonofos
32. Merphos
48. Coumaphos
Zebron
MR-2
30 meter x 0.32 mm x 0.50 m
7HM-G016-17
100 C for 0.5 min to 180 C @ 20 C/min to 240 C @ 6 C/min to
Sample: Analytes are 2 ppm in Dichloromethane. Columns connected using a
5 m Z-Guard Column and a 'Y' splitter.
1. Dichlorvos
17. Fonofos
33. Chlorfenvinphos
2. Mevinphos
18. Phosphamidon Isomer 34. Crotoxyphos
3. Trichlorfon
19. Disulfoton
35. Stirofos
4. TEPP
20. Dichlofenthion
36. Tokuthion
5. Demeton Isomer
21. Phosphamidon
37. Merphos Oxide (Tribufos)
6. Thionazin
22. Chlorpyrifos Methyl
38. Ethion
7. Ethoprop
23. Ronnel
39. Fensulfothion
8. Sulfotep
24. Aspon
40. Contaminant
9. Naled
25. Methyl Parathion
41. Carbophenothion
10. Dicrotophos
26. Malathion
42. Famfur
11. Phorate
27. Fenitrothion
43. EPN
12. Monocrotophos
28. Chlorpyrifos
44. Phosmet
13. Demeton
29. Fenthion
45. Leptophos
14. Terbufos
30. Trichloronate
46. Azinphos Methyl
15. Diazinon
31. Parathion
47. Azinphos Ethyl
16. Dimethoate
32. Merphos
48. Coumaphos
45
37
16,17
44
41
42
45
14
43
17
11
24
21 23
13
10 12
34
31
48
36
35
26
16
18
25
46
39
28
22
27
29
41 42
38
30
19
43
40
33
20
App ID 16181
44
6
1
47
37
47
15
2
8
19
24
12
27,28
20
11
21 23
13
29
25
48
40
34
30
38
36
46
39
35
22
26
10
9
32
33
31,32
App ID 16182
14,15
18
16182
16181
10
12
14
16
18
20
min
10
12
14
16
18
20
min
Nitrogen and Phosphorous Pesticides:

EPA Method 507
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Phenomenex
42,43
2
4
46,47
6
12
23,24
10 11 13
49
45
14
22
17
16 18
20
15
21
19
41
29
27 28
26
34
36
51
39
32
25
35
30
33
50
48
44
40
App ID 15445
Zebron ZB-5ms
30 meter x 0.25 mm x 0.25 m
7HG-G010-11
Split 20:1 @ 250 C, 1 L
100 C to 170 C @ 20 C/min to 210 C @ 4 C/min to 300 C @ 20 C/
min for 4 min
Detector: MSD @ 240 C
Sample:
1. Dichlorvos
19. Profluralin
37. Pendimethalin
2. EPTC
20. Terbufos
38. MGK-264 (Isomer)
3. Mevinphos
21. Pronamide (Propyzamide) 39. Merphos
4. Butylate
22. Diazinon (Dimpylate)
40. Stirofos
5. Vernolate
23. Disulfoton
(Tetrachlorovinphos)
6. Pebulate (Tillam)
24. Terbacil
41. Butachlor
7. Tebuthiuron
25. Metribuzin
42. Napropamide
8. Molinate
26. Alachlor
43. Fenamiphos
9. Propachlor
27. Simetryn
44. Tricyclazole
10. Ethoprop
28. Ametryn
45. Oxadiazon
11. Cycloate
29. Prometryn
46. Oxyfluorfen
12. Chlorpropham
30. Terbutryn
47. Carboxin
13. Trifluralin
31. Bromacil
48. Norflurazon
14. Benefin (Benfluralin) 32. Metolachlor
49. Hexazinone
15. Atraton
33. Triadimefon
50. Fenarimol
16. Prometon
34. Diphenamide
51. Fluridone
17. Atrazine
35. MGK-264
18. Propazine
36. Isopropalin
37
38
31
7
11
13
15
17
19
min
119
environmental
Zebron
35HT
Injector
ECD Detector
Y-Splitter
Chlorinated Herbicides: EPA Method 8151
5m
Z-Guard

11
11
19
1
2
12
15
4 6 7 9 10
20
16
17 18
10
12
14
13
App ID 18788
14
19
21
13
16
min
21
14
5
67
9 10
10
12
16
20
15
17
18
12
14
16
min

Haloacetic Acids: EPA Method 552.2


Injection: Splitless 0:1 @ 250 C, 1 L
Oven Program: 50 C for 0.5 min to 100 C @ 25 C/min to 320 C @
12 C/min for 2 min
Sample: Performed using a 5 m Z-Guard Column and a Y splitter.
Analytes are 50 ppb in isooctane
1. Dalapon
12. Contaminant
2. 3,5-Dichlorobenzoic acid
13. Silvex
3. 4-Nitrophenol
14. Chloramben
4. DCAA (Internal Standard)
15. 2,4,5-T
5. Dicamba
16. 2,4-DB
6. MCPP
17. Dinoseb
7. MCPA
18. Bentazon
8. DBOB (Internal Standard)
19. Picloram
9. Dichlorprop
20. DCPA
18789 21. Acifluorfen
10. 2,4-D
App ID 18789
18788

ECD Detector
Zebron
XLB-HT
Zebron
35HT
Injector

ECD Detector
Y-Splitter
5m
Z-Guard
ECD Detector
Zebron
XLB-HT

Injection: Split 5:1 @ 150 C, 1 L
Oven Program: 40 C for 3 min to 160 C @ 10 C/min
Sample: Analytes are 100 ppb in MTBE
1. Chloroacetic acid
7.
2. Bromoacetic acid
8.
3. Dichloroacetic acid
9.
4. Dalapon
10.
5. Trichloroacetic acid
11.
6. 1,2,3-Trichloropropane18784
18782
Bromochloroacetic acid
Bromodichloroacetic acid
Dibromoacetic acid
Chlorodibromoacetic acid
Tribromoacetic acid
11
7
10
10
9
11
6
1
120
App ID 18782
10
12
14
min
App ID 18784
4
7
10
15
20
25
min
Phenomenex
environmental
Pesticides
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
30 meter x 0.25 mm x 0.20 m
7HG-G017-10
80 C for 0.5 min to 150 C @ 10 C/min to 240 C
@ 4 C/min to 320 C @ 15 C/min for 3 min
11
12
Sample: Analytes were 1 ppm in Dichloromethane
15
20
23
65
56,57
42-44
33
40
63
25
13
26
14
46
28
30
29
18
12
14
34
62
74
60,
61
54
31
83,84
75
59
55
88
72
86
76
91
53
16
18
89
82
85
20
min
22
24
min
98
94
107
110
106
92
100
95
101
111,
112 113
5
2
1
109
102
99
93
104 105
103
108
96,
97
114
10
66,
67
47-49
17
10
71
58
45
21,
22
73 77
51,52
50
27
90
78
70
38-39
App ID 16183
19
68,
69
41
16
87
64
3537
32
79-81
24

1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
Phenomenex
Dichlorvos
EPTC
Butylate
3,5-Dichlorobenzoic acid
(methyl ester)
Vernolate
Pebulate
Mevinphos
4-Nitrophenol (methyl ester)
Mevinphos Isomer
Trichlorfon
Dicamba (methyl ester)
MCPP (methyl ester)
Molinate
Tebuthiuron
MCPA (methyl ester)
DEET
Tetraethyl pyrophosphate
(methyl ester)
Demeton
Dichloroprop (methyl ester)
Trifluralin
Thionazin
Cycloate
Benefin
Propachlor
Ethoprop
Chlorpropham
2,4-D (methyl ester)
10
12
14
16
18
20
22
24
26

28.
29.
31.
32.
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.
50.
51.
52.
53.
54.
55.
56.
Sulfotep
Naled
Dicrotophos
Pentachlorophenol
(methyl ester)
Profluralin
Demeton Isomer
Prometon
Atraton
Monocrotophos
Atraton Isomer
Silvex (methyl ester)
Terbufos
Propazine
Diazinon
Pronamide
Atrazine
Simazine
Terbuthylazine
Dioxathion
Fonofos
Dimethoate
2,4,5-T Methyl ester
Disulfoton
Chloramben (methyl ester)
Phosphamidon Isomer
Secbumeton
Dichlofenthion
2,4-DB (methyl ester)
28
30
32
34
min
26

57.
58.
59.
60.
61.
62.
63.
64.
65.
66.
67.
68.
69.
70.
71.
72.
73.
74.
75.
76.
77.
78.
79.
80.
81.
82.
83.
84.
85.
Terbacil
Dinoseb (methyl ester)
Alachlor
Chlorpyrifos methyl
Phosphamidon
Ronnel
Prometryn
Ametryn
Bentazon (methyl ester)
Aspon
Simetryn
Metribuzin
Methyl parathion
Terbutryn
Metolachlor
Malathion
DCPA
Fenitrothion
Chlorpyrifos
Trichloronate
Triadimeton
Pichloram (methyl ester)
Isopropalin
Fenthion
MGK-264 Isomer
Parathion
Merphos
Bromacil
Clofenvinfos Isomer
28
30
32

86.
87.
88.
89.
90.
91.
92.
93.
94.
95.
96.
97.
98.
99.
100.
101.
102.
103.
104.
105.
106.
107.
108.
109.
110.
111.
112.
113.
114.
MGK-624
Pendimethalin
Diphenamide
Clofenvinfos
Butachlor
Crotoxyphos
Stirofos
Tokuthion
Oxadiazon
Merphos Oxide
Napropamide
Fenamiphos
Oxyfluorfen
Acifluorfen
Carboxin
Ethion
Tricyclazole
Fensulfothion
Carbophenothion
Famfur
Norflurazon
Hexazinone
EPN
Phosmet
Leptophos
Azinphos-Methyl
Fenarimol
Azinphos-ethyl
Coumaphos
min
121
environmental
Pesticides
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
30 meter x 0.25 mm x 0.25 m
7HG-G016-11
Sample: Analytes were 1 ppm in Dichloromethane
21
24
25
36,37 41-43
39
44-45
84
80-81
64-66
61
71-74
63
20 23
38
77,78
52
31
29 30
60
18
62
59
32
48
34
28
82
76
49
27
19
85
50
40
22
75
46-47
35
26
79
69
33
86
67
58
68
87
70
51
83
14
16
min
18
22
min
24
17
13-15
57
94,95
12
96
6,7
3
55
56
5
10
1
2
16
88
91 92,
93
101
108
107
97 99 103
100 102
90
54
53
104
106
110,111
109
9 11
98
Peak No.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
122
Sample Analyte
Dichlorvos
DEET
EPTC
3,5-Dichlorobenzoic acid
(methyl ester)
Butylate
4-Nitrophenol
(methyl ester)
Vernolate
Mevinphos
Mevinphos Isomer
Pebulate
Trichlorfon
Dicamba (methyl ester)
Molinate
Tebuthiuron
MCPP (methyl ester)
Tetraethyl pyrophosphate
(methyl ester)
MCPA (methyl ester)
Demeton Isomer
Thionazin
Dichloroprop (methyl ester)
Propachlor
Cycloate
Ethoprop
Trifluralin
Benefin
2,4-D (methyl ester)
Peak No.
27.
28.
29.
30.
31.
32.
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.
50.
51.
52.
53.
54.
10
12
Sample Analyte
Sulfotep
Naled
Chlorpropham
Dicrotophos
Phorate
Monocrotophos
Pentachlorophenol
(methyl ester)
Demeton
Atraton
Profluralin
Prometon
Silvex (methyl ester)
Terbufos
Dimethoate
Simazine
Propazine
Atrazine
Diazinon
Dioxathion
Terbuthylazine
Fonofos
Pronamide
Chloramben (methyl ester)
2,4,5-T Methyl ester
Phosphamidon Isomer
Disulfoton
Secbumeton
Terbacil
14
16
18
Peak No.
55.
56.
57.
58.
59.
60.
61.
62.
63.
64.
65.
66.
67.
68.
69.
70.
71.
72.
73.
74.
75.
76.
77.
78.
79.
80.
81.
82.
83.
20
22
24
26
Sample Analyte
Dinoseb (methyl ester)
Dichlofenthion
2,4-DB (methyl ester)
Phosphamidon
Chlorpyrifos methyl
Alachlor
Bentazon (methyl ester)
Ronnel
Prometryn
Methyl parathion
Ametryn
Simetryn
Aspon
Metribuzin
Terbutryn
Malathion
Fenitrothion
Pichloram (methyl ester)
Metolachlor
Chlorpyrifos
DCPA
Bromacil
Fenthion
Trichloronate
Triadimeton
Isopropalin
Parathion
MGK-624
Merphos
28
30
32
Peak No.
84.
85.
86.
87.
88.
89.
90.
91.
92.
93.
94.
95.
96.
97.
98.
99.
100.
101.
102.
103.
104.
105.
106.
107.
108.
109.
110.
111.
112.
34
112
App ID 16184
105
89
min
Sample Analyte
Pendimethalin
Diphenamide
MGK-264 Isomer
Clofenvinfos
Crotoxyphos
Butachlor
Stirofos
Tokuthion
Napropamide
Fenamiphos
Merphos oxide
Oxadiazon
Oxyfluorfen
Carboxin
Tricyclazole
Acifluorfen
Ethion
Fensulfothion
Carbophenothion
Famfur
Norflurazon
Hexazinone
EPN
Phosmet
Leptophos
Azinphos-Methyl
Fenarimol
Azinphos-ethyl
Coumaphos
Phenomenex
environmental
Volatile Organics: EPA Method 8260B
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Zebron ZB-624
20 meter x 0.18 mm x 1.00 m
7FD-G005-22
Purge and Trap
Constant Flow Helium
35 C to 210 C
MSD; 35-275 amu
72,73
56
54,55
Under 10 min!
57,58
60
64,65
30,31
45
19-21
67
69 71
75
70
66
80
68
32-34
48
53
23-25
3 4
38
22
13
26
15
36
39
41
47
81
61
51
59
52
6
2
50
82
46
40
49
42
35
14
11
76,77
79
44
29
12
5
2
27,28
62,63
78
6
App ID 15866
9,10
74
43
37
16-18
min
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
Phenomenex
Chloromethane
Vinyl chloride
Bromomethane
Chloroethane
Trichlorofluoromethane
Ethanol
Dichlorotrifluoroethane
Acrolein
Trichlorotrifluoroethane
1, 1-Dichloroethene
Acetone
Methyl iodide
Carbon disulfide
Methylene chloride
t-Butanol
trans-1,2-Dichloroethane
Methyl-t-butyl ether
Acrylonitrile
1, 1-Dichloroethane
Vinyl Acetate
Diisopropyl ether
Ethyl-t-butyl ether
2, 2-Dichloropropane
cis-1, 2-Dichloroethene
2-Butanone
Bromochloromethane
Chloroform
Tetrahydrofuran

29.
30.
31.
32.
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.
50.
51.
52.
53.
54.
55.
56.
1, 1, 1-Trichloroethane
1, 1-Dichloropropene
Carbon tetrachloride
1, 2-Dichloroethane-d4
Benzene
1, 2-Dichloroethane
t-Amyl methyl ether
Fluorobenzene
Trichloroethene
Dibromomethane
Bromodichloromethane
2-Chloroethylvinyl ether
cis-1, 3-Dichloropropene
Methyl isobutyl ketone
Toluene-d8
Toluene
trans-1, 3-Dichloropropene
1, 1, 2-Trichloroethane
Tetrachloroethene
2-Hexanone
Dibromochloromethane
Ethylene dibromide
Chlorobenzene
1, 1, 1, 2-Tetrachloroethane
Ethylbenzene
m, p-Xylene

57.
58.
59.
60.
61.
62.
63.
64.
65.
66.
67.
68.
69.
70.
71.
72.
73.
74.
75.
76.
77.
78.
79.
80.
81.
82.
o-Xylene
Styrene
Bromoform
Isopropylbenzene
4-Bromofluorobenzene
1, 1, 2, 2-Tetrachloroethane
Bromobenzene
1, 2, 3-Trichloropropane
n-Propylbenzene
2-Chlorotoluene
1, 3, 5-Trimethylbenzene
4-Chlorotoluene
tert-Butylbenzene
1, 2, 4-Trimethylbenzene
sec-Butylbenzene
1, 3-Dichlorobenzene
4-Isopropyltoluene
n-Butylbenzene
1, 2-Dichlorobenzene-d4
1, 2-Dibromo-3-chloropropane
1, 2, 4-Trichlorobenzene
Hexachlorobutadiene
Naphthalene
1, 2, 3-Trichlorobenzene
123
environmental
Fast H53 Analysis of Diesel Fuel

Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Nonhalogenated Organics Using GC/FID:

EPA Method 8015B
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:

15 meter x 0.32 mm x 0.10 m
7EM-G015-02
On-Column @ 63 C, 0.1 L
60 C to 375 C @ 25 C/min
FID @ 400 C
Diesel Fuel was 200 ppm in Dichloromethane with
Internal Standards at 50 ppm
1. Decane (C10)
Zebron ZB-5
30 meter x 0.53 mm x 1.50 m
7HK-G002-28
Split 20:1 @ 225 C, 1 L
45 C for 3 min to 60 C at 5 C/min to 275 C at 20 C/min
FID @ 350 C
All analytes at 100 g/mL
1. Methanol (solvent)
8. Isobutanol
2. Ethanol
9. 1,4-Dioxane
3. Acetonitrile
10. Methylmethacrylate
4. Diethyl ether
11. 4-Methyl-2-Pentanone [MIBK]
5. Propanenitrile
12. Impurity
6. 2-Butanone [MEK]
13. Ethylmethacrylate
7. 2-Methyl-2-propanenitrile
14. Acrylamide
34
7
13
8
11
14
10
12 min
App ID 14772
App ID 16147
9
12
10 min
Phenols
Aroclor 1232: EPA Method 8082
10
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-5MSi
30 meter x 0.25 mm x 0.25 m
7HG-G018-11
Split 70:1@ 210 C, 1 L
120 C for 1 min to 300 C at 9 C/min hold until last
peak elutes
Sample: 1. Tetrachloro-m-xylene (Surrogate)
2. Decachlorobiphenyl (Surrogate)
Zebron ZB-5MSi
30 meter x 0.25 mm x 0.25 m
7HG-G018-11
Split 5:1@ 240 C, 1 L
60 C to 140 C @ 5 C/min to 280 C @ 10 C/min
MSD @ 230 C; 45-450 amu
1. Phenol
15. Benzoic Acid
2. 2-Chlorophenol
3. 2-Methylphenol
4. 4-Methylphenol
5. 3-Methylphenol
7. 2-Nitrophenol
8. 2-Ethylphenol
23. 4-Nitrophenol
12. 4-Ethylphenol
13. 3-Ethylphenol
27. Dinoseb
13,14,15
124
10
12
14
16
18
20
22
24 min
4,5
12
12 16
1718
24
19
20
21
22
10
12
14
16
18
26
25
23
20
22
App ID 16410
App ID 16416
9,10,11
27
min
Phenomenex
toxicology / DRUGS OF ABUSE

Drugs of Abuse Screen
Carboxy-THC
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Benzoylecgonine
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-Drug-1
10 meter x 0.18 mm x 0.18 m
7CD-G023-08
Split 10:1 @ 280 C, 0.1 L
220 C to 340 C @ 20 C/min
MSD @ 320 C
Analytes are 1.5 ppm in BSTFA
1. Carboxy-THC-d3
2. Carboxy-THC
Zebron ZB-Drug-1
10 meter x 0.18 mm x 0.18 m
7CD-G023-08
Split 10:1 @ 260 C, 1 L
235 C to 320 C @ 25 C/min
MSD @ 230 C
Analytes are 24 ppm in BSTFA
1. Benzoylecgonine-d3
2. Benzoylecgonine
17805
17801
1,2
2.5
2.6
2.7
2.8
2.9
3.0
3.1
3.2
3.3
3.40
3.5
3.6
3.7
3.8
3.9
min
PCP (Phencyclidine)
App ID 17805
2.6
2.7
2.8
2.9
3.0
3.1
3.2
3.3
3.4
3.5
3.6
3.7
3.8
3.9
4.0
min
Expanded Amphetamines and Interferences

Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-Drug-1
10 meter x 0.18 mm x 0.18 m
7CD-G023-08
Split 10:1 @ 260 C,1 L
70 C to 120 C @ 20 C/min to 180 C @
5 C/min to 300 C @ 30 C/min
Detector: MSD @ 320 C; SIM
Sample: Analytes are 20 ppm derivatized with HFBA
1. Norephedrine
9. Pseudoephedrine
2. Norpseudoephedrine
10. MDA-d5
3. Phentermine
11. MDA
4. Amphetamine-d11
12. MDMA-d5
5. Amphetamine
13. MDMA
6. Ephedrine
14. TFMPP
7. Methamphetamine-d14
15. MDEA-d5
8. Methamphetamine
16. MDEA
17800
1,2
Zebron ZB-Drug-1
10 meter x 0.18 mm x 0.18 m
7CD-G023-08
Split 10:1 @ 280 C, 0.5 L
160 C to 340 C @ 20 C/min
MSD @ 230 C
Analytes are 2 ppm in Ethyl acetate
1. Phencyclidine-d5
2. Phencyclidine
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
2.5
App ID 17801
1,2
18039
2,3,4
6
8
2.0
2.2
2.4
Phenomenex
2.6
2.8
3.0
3.2
3.4
3.6
3.8
4.0
4.2
4.4
4.6
min
13
12
14
11
10
2
10
11
12
App ID 18039
App ID 17800
16
15
min
125

Drugs of Abuse Screen (cont'd)
Opiates
Expanded Healthcare Opiates
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-Drug-1
10 meter x 0.18 mm x 0.18 m
7CD-G023-08
Split 10:1 @ 260 C, 1 L
180 C to 340 C @ 20 C/min
MSD @ 320 C
Analytes are 24 ppm derivatized with
1. Hydroxylamine 2. BSTFA
1. Morphine-d3
2. Morphine
3. Codeine-d6
4. Codeine
5. Oxymorphone
6. Hydromorphone-d6
7. Hydromorphone
8. Oxycodone-d6
9. Oxycodone
10. Hydrocodone
18200
17899
7
10
13,14 17
5,6
3
8
1,2
15,16
3.9
4.0
4.1
4.2
4.3
4.4
4.5
4.6
4.7
4.8
4.9
5.0
5.1
5.2
5.3
5.4
App ID 17899
1,2
min
10
9
2.5
3.5
4.5
11
12
5.5
18
6.5
App ID 18200
4
3
7 min
Zebron ZB-Drug-1
10 meter x 0.18 mm x 0.18 m
7CD-G023-08
Split 10:1 @ 260 C,1 L
150 C to 320 C @ 25 C/min for 5 min
MSD @ 320 C; SIM
Analytes are 12 g/mL derivatized with propionic anhydride
(tramadols are 24 g/mL)
1. Meperidine-d4
10. Codeine
2. Meperidine
11. Oxycodone-d6
3. Tramadol
12. Oxycodone
4. O-Desmethyltramadol
13. Hydromorphone-d6
5. Normeperidine-d4
14. Hydromorphone
6. Normeperidine
15. Morphine-d3
7. N-Desmethyltramadol
16. Morphine
8. Hydrocodone
17. Oxymorphone
9. Codeine-d6
18. Norcodeine
6-MAM
Barbiturates
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-Drug-1
10 meter x 0.18 mm x 0.18 m
7CD-G023-08
Split 10:1 @ 260 C, 1 L
245 C to 300 C @ 25 C/min
MSD @ 300 C
Analytes are 1 ppm derivatized with
BSTFA
1. 6-MAM-d3
2. 6-MAM
17802
Zebron ZB-Drug-1
10 meter x 0.18 mm x 0.18 m
7CD-G023-08
Split 10:1 @ 260 C, 1 L
145 C to 300 C @ 25 C/min
MSD @ 230 C
Analytes are 12 ppm derivatized with TMPAH
1. Butalbital-d5
2. Butalbital
3. Amobarbital
4. Pentobarbital
5. Secobarbital
6. Phenobarbital-d5
7. Phenobarbital
17810
4
1,2
3
1, 2
App ID 17802
App ID 17810
2.0
126
2.2
2.4
2.6
2.8
3.0
3.2
3.4
3.6
3.8
4.0
4.2
min
1.4
1.6
1.8
2.0
2.2
2.4
2.6
2.8
3.0
3.2 min
Phenomenex

Blood Alcohols
Column: Zebron ZB-BAC1
Part No.: 7HK-G021-36
Blood Alcohols
Zebron
BAC1
Injector
Column: Zebron ZB-BAC2

Part No.: 7HK-G022-32
FID Detector
Y-Splitter
5m
Z-Guard
FID Detector
Zebron
BAC2
17982
2
5
1
7
8
8
1
min
Drug Screen
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-Drug-1
10 meter x 0.18 mm x 0.18 m
7CD-G023-08
Split 10:1 @ 260 C,1 L
50 C to 150 C @ 15 C/min to 240 C @ 7 C/min to 320 C @ 25 C/min
for 2 min
Sample: Analytes are 25 ppm in Methanol
1. Acetophenone
15. Meprobamate
29. Trimipramine
2. Nicotine
16. Diphenhydramine 30. Chlorcyclizine
3. Benzocaine
17. Lidocaine
31. Cocaine
4. Ibuprofen
18. Hexobarbital
32. Desipramine
5. Allobarbital
19. Doxylamine
33. Codeine
6. Aprobarbital
20. Glutethimide
34. Morphine
7. Butalbital
21. Caffeine
35. Diazepam
8. Amobarbital
22. Chlorpheniramine 36. Hydrocodone
9. Phenacetin
23. Methapyrilene
37. 6-MAM
10. Pentobarbital
24. Phenobarbital
38. Oxymorphone
11. Acetaminophen 25. Procaine
39. Heroin
12. Benzphetamine
26. Methadone
40. Fentanyl
13. Secobarbital
27. Bromopheniramine 41. Ibogaine
14. Phencyclidine
28. Propoxyphene
42. Triazolam
43. LSD
1
6,7
4,5
17808
Zebron ZB-Drug-1
10 meter x 0.18 mm x 0.18 m
7CD-G023-08
Split 20:1 @ 280 C, 0.1 L
200 C to 210 C @ 20 C/min to
320 C @ 30 C for 1 min
Sample: Analytes are 24 ppm derivatized with MTBSTFA
1. Diazepam
7. Temazepam
2. Nordiazepam-d5
8. Clonazepam
3. Nordiazepam
9. Lorazepam
4. Oxazepam-d5
10. Alprazolam
5. Oxazepam
11. -Hydroxyalprazolam-d5
6. Temazepam-d5
12. -Hydroxyalprazolam
2,3
min
Benzodiazepines
App ID 17982
App ID 17983

Injection: Split 0.8:1 @ 150 C, 1 mL
Carrier Gas: Helium @ 80 cm/sec (constant flow)
Oven Program: 40 C Isothermal
Sample: Analytes 0.025 % and internal standards 0.100 % in water
1. Methanol
17983
2. Acetaldehyde
3. Ethanol
2
4. Isopropanol
6
5. Acetone
6. t-Butanol
7. n-Propanol
8. 2-Butanol
18175
17
22,23
41
11,12
8
2.2
2.4
2.6
Phenomenex
2.8
3.2
3.4
10
3.6
3.8
4.2
4.4
4.6
4.8
App ID 17808
14 18,19
5 min
4
3
5
12
9 13
6 7 8 10
11
10
20
21
12
33
30
29
26,27
28
24
35
34 36
31
25
14
43
37
32
16
42
40
39
38
18
20
22
App ID 18175
15,16
min
127
pharmaceuticals
USP Glycerin

Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:

Procedure A - Class 1
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-624
30 meter x 0.53 mm x 3.00 m
7HK-G005-36
Split 10:1 @ 220 C, 0.5 L
100 C to 220 C @ 7.5 C/min for 4 min
FID @ 250 C
1. Diethylene Glycol
2. Glycerin
Zebron ZB-624
30 meter x 0.32 mm x 1.80 m
7HM-G005-31
Headspace 5:1 @ 140 C, 1 mL
40 C for 20 min to 240 C @ 10 C/min
for 20 min
Sample: Sample Prepared as per USP Guidelines
4. Benzene
1
4
3
4
min

Procedure A - Class 2 Mix A
App ID 16348
App ID 15789
10
12
min

Procedure A - Class 2 Mix B
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-624
30 meter x 0.32 mm x 1.80 m
7HM-G005-31
40 C for 20 min to 240 C @ 10 C/min
for 20 min
Sample: Sample Prepared as per USP Guidelines
1. Methanol
8. 1,4-Dioxane
2. Acetonitrile
9. Toluene
3. Dichloromethane
10. Chlorobenzene
4. cis-1,2-Dichloroethene
11. Ethylbenzene
5. THF
12. m-Xylene
6. Cyclohexane
13. p-Xylene
7. Methylcyclohexane
14. o-Xylene
Zebron ZB-624
30 meter x 0.32 mm x 1.80 m
7HM-G005-31
FID @ 250 C
Sample Prepared as per USP Guidelines
1. Hexane
2. Nitromethane
3. Chloroform
4. 1,2-Dimethoxyethane
5. Trichloroethylene
6. Pyridine
7. Methylbutylketone
8. Tetralin
8
9
10
11
14
12
13
2
5
128
10
15
3
2
8
20
25
30 min
App ID 16350
App ID 16349
4
10
20
30
min
Phenomenex
pharmaceuticals
Procedure B - Class 1
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:

Procedure B - Class 2 Mix A
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-WAXPLUS
30 meter x 0.32 mm x 0.25 m
7HM-G013-11
50 C hold 20 min to 165 C @ 6 C/min hold 20 min
FID @ 250 C
Sample prepared as per USP method
4. Benzene
Zebron ZB-WAXPLUS
30 meter x 0.32 mm x 0.25 m
7HM-G013-11
FID @ 250 C
1. Cyclohexane
8. Toluene
9. 1,4-Dioxane
3. THF
10. Ethylbenzene
4. Methanol
11. p-Xylene
5. Dichloromethane
12. m-Xylene
6. cis-1,2-Dichloroethene
13. o-Xylene
7. Acetonitrile
14. Chlorobenzene
12
1
4
12
2, 3
4
1
min
10
11
14
13
7
App ID 16353
App ID 16352
9
4
10
min
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:

Procedure B - Class 2 Mix B

Procedure B - Class 2 Mix C
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-WAXPLUS
30 meter x 0.32 mm x 0.25 m
7HM-G013-11
FID @ 250 C
1. Hexane
5. Methylbutylketone
6. Nitromethane
7. Pyridine
4. Chloroform
8. Tetralin
Zebron ZB-WAXPLUS
30 meter x 0.32 mm x 0.25 m
7HM-G013-11
FID @ 250 C
1. N,N-Dimethylformamide
2. N,N-Dimethylacetamide
7
2
App ID 16355
App ID 16354
6
5
Phenomenex
10
15
20
25
30 min
10
20
2
30
min
129
food, flavors, fragrances

Food Industry FAMEs
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-WAX
30 meter x 0.25 mm x 0.25 m
7HG-G007-11
Split 5:1 @ 220 C, 1 L
60 C for 2 min to 150 C @ 13 C/min to 240 C
@ 2 C/min
Sample: 133-266 ppm in methylene chloride
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
35.
36.
Methyl Butyrate (C4:0)

Methyl Hexanoate (C6:0)
Methyl Octanoate (C8:0))
Methyl Decanoate (C10:0)
Methyl Undecanoate (C:11)
Methyl Laurate (C12:0)
Methyl Tridecanoate (C13:0)
Methyl Myristate (C14:0)
Myristoleic acid methyl ester (C14:1)
Methyl Pentadecanoate ( C15:0)
cis-10-Pentadecenoic acid methyl ester (C15:1)
Methyl Palmitate (C16:0)
Palmitoleic acid methyl ester (C16:1)
Methyl Heptadecanoate (C17:0)
cis-10-Heptadecenoic acid methyl ester (C17:1)
Methyl Stearate (C18:0)
Oleic acid methyl ester (C18:1n9c)
Elaidic acid methyl ester (C18:1n9t)
Linoleic acid methyl ester (C18:2n6c)
Linolelaidic acid methyl ester (C18:2n6t)
g-Linolenic acid methyl ester (C18:3n6)
Linolenic acid methyl ester (C18:3n3)
Methyl Arachidate (C20:0)
cis-11-Eicosenoic acid methyl ester (C20:1)
cis-11-14-Eicosadienoic acid methyl ester (C20:2)
cis-8,11,14-Eicosatrienoic acid methyl ester (C20:3n6)
Methyl Heneicosanoate (C21:0)
Arachidonic acid methyl ester (C20:4n6)
cis-11,14,17-Eicosatrienoic acid methyl ester (C20:3n3)
cis-5,8,11,14,17-Eicosapentaenoic acid methyl ester (C20:5n3)
Methyl Behenate (C22:0)
Erucic acid methyl ester (C22:1)
cis-13,16-Docosadienoic acid methyl ester (C22:2)
Methyl Tricosanoate (C23:0)
Methyl Lignocerate (C24:0)
cis-4,7,10,13,16,19-Docosahexaenoic acid
methyl ester (C22:6n3)
37. Nervonic acid methyl ester (C24:1)
16320
1
12
16
9
17,18
23
10 11
13
10
15
20
19 21 22
20
31
24
27
25 26 28 29 30
32
35
36
33 34
37
30
40
min
14
App ID 16320
Glenfiddich Scotch Whiskey

Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Glenfiddich Scotch Whiskey

Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-WAXPLUS
30 meter x 0.25 mm x 0.25 m
7HG-G013-11
Split 30:1 @ 140 C, 0.2 L
FID @ 280 C
Injection of pure Glenfiddich
1. Acetaldehyde
6. 1-Propanol
2. Ethyl Acetate
7. Isobutanol
3. Acetal
8. Isoamyl alcohol
4. Methanol
9. 2-Methylbutanol
5. Ethanol
Zebron ZB-624
20 meter x 0.18 mm x 1.00 m
7FD-G005-22
Split 40:1 @ 140 C, 0.2 L
35 C for 4 min to 85 C @ 10 C/min to 200 C @ 25 C/min
for 1 min
Sample: Injection of pure Glenfiddich
1. Acetaldehyde
6. Isobutanol
2. Methanol
7. Acetal
3. Ethanol
8. Isoamyl alcohol
4. 1-Propanol
9. 2-Methylbutanol
5. Ethyl Acetate
8,9
App ID 16044
130
App ID 16046
3
4
6
4
1
10
min
5
7
2
2
10
12
14min
Phenomenex

Flavors
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Vitamin E and Sterols

Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-FFAP
30 meter x 0.25 mm x 0.25 m
7HG-G009-11
Split 100:1 @ 250 C, 1 L
MSD @ 270 C
1. Acetone
10. Acetic Acid
2. Ethyl Acetate
11. Decanal
3. Ethanol
12. Propylene Glycol
4. Decane
13. Ethyl Decanoate
5. Ethyl Butyrate
14. Neral
6. Limonene
15. a-Terpineol
7. 2,3-Dimethylpyrazine 16. Neryl Acetate
8. (z)-3-Hexenol
17. Geranial
9. Tetradecane
18. Valeric Acid
Zebron ZB-5
30 meter x 0.25 mm x 0.10 m
7HG-G002-02
for 6 min to 340 C @ 10 C/min for 15.8 min
Sample: 1. Squalene
8. g-Tocomonoenol
2. FFA C24:0
9. Stigmasta-3,5-diene
3. d-Tocopherol
10. Cholesterol
4. d-Tocomonoenol
11. a-Tocopherol
5. Campesta-3,5-diene
12. Campesterol
6. -Tocopherol
13. Stigmasterol
7. Stigmasta-3,5,22-triene
14. b-Sitosterol
19. Decanol
20. Nonanoic Acid
21. Decanoic Acid
22. Vanillin
23. Anthracene
1
6
23
7 8
11
20
17
14
12
21
10
22
18
10
15
20
25
8
4
18
20
8
5
1
9,10
12 13
3
2
4
12
15
15
Phenomenex
20
25
11
30
35
40
13
10
14
App ID 14856
10
min
Zebron ZB-FFAP
60 meter x 0.25 mm x 0.25 m
7KG-G009-11
Split 40:1 @ 220 C, 0.2 L
FID @ 250 C
1. Myristic Acid
10. Linoleic Acid
2. Myristoleic Acid
11. Linolenic Acid
3. Palmitic Acid
12. Arachidic Acid
4. Palmitelaidic Acid
13. Gondonic Acid (C15)
5. Palmitoleic Acid
14. Behenic Acid (C17)
6. Stearic Acid
15. Erucic Acid (C19)
7. Elaidic Acid
8. Oleic Acid
9. Linolelaidic Acid
7,8
5
4
24
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
1
3
22
Unsaturated Free Fatty Acids
Zebron ZB-FFAP
60 meter x 0.25 mm x 0.25 m
7KG-G009-11
Split 40:1 @ 220 C, 0.2 L
FID @ 250 C
1. Methyl Myristate
10. Methyl Linelaidate
2. Methyl Myristoleate
11. Methyl Linolenate
3. Methyl Palmitate
12. Methyl Arachidate
4. Methyl Palmitelaidate
13. Methyl Gondonate
5. Methyl Palmitoleate
14. Methyl Behenate
6. Methyl Stearate
15. Methyl Erucate
7. Methyl Oleate
8. Methyl Elaidate
9. Methyl Linoleate
9 10
5 7
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
11
30 min
Unsaturated Fatty Acids Methyl Esters (FAMEs)
12 13
App ID 16331
16
19
15
45 min
11
14 15
10
15
20
25
App ID 14858
13
14
App ID 14899
30
35
40
min
131

Ylang Ylang Oil
Rosemary Oil
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-1ms
10 meter x 0.10 mm x 0.10 m
7CB-G011-02
Split 120:1 @ 160 C, 0.2 L
60 C to 120 C @ 15 C/min to 160 C @ 5 C/min to 220 C
@ 20 C/min
Sample: Oil was 10 % in dichloromethane
1. p-Methyl anisole 7. Copaene
13. Farnasene
2. Methyl benzoate 8. -Caryophyllene 14. d-Cadinene
3. Linalool
9. Cinnamyl acetate 15. Farnesol
4. Benzyl acetate
10. Humulene
16. Benzyl benzoate
5. Geraniol
11. Germacrene
17. Benzyl salicylate
6. Geranyl acetate
12. a-Amorphene
18. Farnesyl acetate
8,9
Zebron ZB-1ms
10 meter x 0.10 mm x 0.10 m
7CB-G011-02
Split 120:1 @ 160 C, 0.2 L
for 2 min
Sample: Sample was 10 % in dichloromethane
1. Tricyclene
8. Limonene
15. 4-Terpineol
2. -Thujene
9. g-Terpinene 16. Terpineol
3. -Pinene
10. Terpinolene
17. Bornyl Acetate
4. Camphene
11. Linalool
18. Eugenol
5. -Pinene
12. Camphor
19. Copaene
6. -Myrcene 13. Isoborneol
20. Caryophyllene
7. Eucalyptol
14. Borneol
21. -Caryophyllene

11
3
12
7,8
20
14
10
1
2
16
18
12
18
17
15
1
2
10
11
12
13
14
15 min
TMS Sugars
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
11
21
15
13
10
17
19
18
App ID 16070
13
14
App ID 16053
6
3
10
11
12
13
14
min
Cold-Pressed Orange Oil

Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
30 meter x 0.25 mm x 0.25 m
7HG-G016-11
Split 100:1 @ 225 C, 1 L
200 C for 6 min
FID @ 250 C
Sugar mix concentration 176 ppm
1. d-(-)-Arabinose
2. d-Ribose (isomers)
3. d-Xylose
4. d-Mannose
5. d-(+)-Galactose
6. d-Glucose
7. d-(+)-Glucose
Zebron ZB-1ms
10 meter x 0.10 mm x 0.10 m
7CB-G011-02
Split 120:1 @ 160 C, 0.2 L
60 C to 130 C @ 10 C/min to 280 C @ 30 C/min for 3 min
MSD
Sample was 10 % in dichloromethane
10. trans-Limonene oxide
1. -Pinene
11. Citronellal
2. -Phellandrine
12. -Terpineol
3. -Myrcene
13. Decanal
4. Octanal
14. Carvone
5. 3-Carene
15. Neral
6. Limonene
16. Geranial
7. Nonanal
17. -Cubebene
8. Linalool
18. -Cubebene
9. cis-Limonene oxide
19. Dodecanal
20. Valencene
21. Cadinene
22. Nootkatone
3,4
6
1
7,8
13
2.0
132
3.0
4.0
5.0 min
16
10 12
11
14,15
20
21
17 18 19
App ID 16016
App ID 16248
9
2
22
10
min
Phenomenex
Food Safety
Glycols and Benzene in Toothpaste Standard
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Phthalate Esters: EPA Method 606

Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-WAXplus
30 meter x 0.25 mm x 0.25 m
7HG-G013-11
Split 20:1 @ 225 C, 0.5 L
FID @ 250 C
Analytes at 0.5 mg/mL
1. Benzene
2. Acetonitrile
3. 1,2-Propanediol
4. Ethyleneglycol
5. 1,3-Propanediol (IS)
6. Diethyleneglycol
7. Glycerol
8. Triethyleneglycol
Zebron ZB-5ms
30 meter x 0.32 mm x 0.25 m
7HM-G010-11
Split 20:1 @ 300 C, 1 L
FID @ 300 C
1. Dimethyl Phthalate
2. Diethyl Phthalate
3. Di-n-butyl Phthalate
4. Butyl Benzyl Phthalate
5. Bis(2-ethylhexyl) Phthalate
6. Di-n-octyl Phthalate
5
15425
5
4
10
App ID 15425
App ID 16510
8
7
min
Melamine in Baby Formula
10
12
14
16
min
Melamine in Baby Formula

Zebron ZB-XLB-HT Inferno
15 meter x 0.25 mm x 0.25 m
7EG-G024-11
100 C for 0.5 min to 320 C @ 25 C/min
MSD @ 325 C
Analytes are 200 ng / 100 L in BSTFA / Pyridine (1:1)
1. Cyanuric Acid 13C3 (IS)
2. Cyanuric Acid
3. Melamine 13C3 15N3 (IS)
4. Melamine
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-5ms
30 meter x 0.25 mm x 0.25 m
7HG-G010-11
75 C for 1 min to 320 C @ 15 C/min for 2.67 min
MSD @ 320 C
Analytes were derivatized with BSTFA in
pyridine as per FDA Screeing Method
1. Cyanuric Acid
2. Ammelide
3. DACP (IS)
4. Ammeline
5. Melamine
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
18265
3,4
App ID 18488
Complete analysis
in less than half
the time of the
FDA method
1
6
Phenomenex
2
10
4
11
12
13
14
15
16
min
17
18
min
App ID 18265
1,2
7 min
133
industrial chemicals
Polynuclear Aromatic Hydrocarbons (PAHs)
Aromatics
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-5 with 5 meter Guardian

30 meter x 0.25 mm x 0.25 m
7HG-G002-11-GGA
Split 15:1 @ 260 C, 1 L
170 C to 240 C @ 15 C/min to 275 C @ 4 C/min to 320 C
@ 10 C/min for 5 min
Sample: All analytes are 100 g/mL in methylene chloride
1. Naphthalene
10. Chrysene
2. Acenaphthylene
3. Acenaphthene
4. Fluroene
13. Benzo[a]pyrene
5. Phenanthrene
6. Anthracene
7. Fluoranthene
8. Pyrene
Zebron ZB-WAX
30 meter x 0.25 mm x 0.25 m
7HG-G007-11
Split 150:1 @ 250 C,1 L
Helium @ 25 cm/sec @ 40 C
40 C (4 min) to 140 C @ 6 C/min
FID @ 300 C
200 ppm in Methanol
1. p-Xylene
7. sec-Butylbenzene
2. Isopropylbenzene
8. 2-Chlorotoluene
3. o-Xylene
9. 4-Chlorotoluene
4. n-Propylbenzene
10. 1, 3-Dichlorobenzene
5. Chlorobenzene
6. tert-Butylbenzene
1
15
10
9
14
12
6 7
23 4
16
89
13
11
12
10
13
16
19
App ID 1307
App ID 14942
10 11
min
12
16
20 min
BTEX

Styrene Impurites:
ASTM Method D 5135-95
3
1
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-WAX
30 meter x 0.32 mm x 0.50 m
7HM-G007-17
Split 20:1 @ 225 C, 0.2 L
60 C to 75 C @ 15 C/min to 90 C @ 3 C/min (hold 3 min)
FID @ 300 C
1. Pentane
2. Heptane
3. Solvent (methylene chloride)
4. Benzene
5. Decane
6. Toluene
7. Ethylbenzene
8. p-Xylene
9. m-Xylene
10. Dodecane
11. o-Xylene
Zebron ZB-WAXplus
30 meter x 0.25 mm x 0.25 m
7HG-G013-11
Split 100:1@ 250 C,1 L
35 C for 2.5 min to 85 C @ 10 C/min for 1 min
FID @ 225 C
1. Methyl Formate
10. 2-Butanol
2. Acetone
11. Toluene
3. Ethyl Acetate
12. n-Propanol
4. Methyl Ethyl Ketone
13. Ethyl Benzene
5. Methanol
14. p-Xylene
6. 2-Methyl-2-propanol
15. m-Xylene
7. Methylene Chloride
16. 1-Butanol
8. Benzene
17. o-Xylene
9. Ethanol
15815
9
7 8
11
10
13 14
11
15
17
2
4
16
6
10
3
9
12
App ID 15815
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
App ID 14895
1.5
134
2.5
3.5
4.5
5.5
6.5 min
min
Phenomenex
Industrial Solvents
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-624
60 meter x 0.32 mm x 1.80 m
7KM-G005-31
Split 20:1 @ 220 C, 0.6 L
40 C for 8 min to 90 C @ 8 C/min to 210 C @ 15 C
for 8 min
Sample: All analytes were 140 ng on column except methylene

chloride as the solvent
1. Methanol
20. Benzene
2. Pentane
21. Heptane
3. Ethanol
4. Ether
23. 1,4-Dioxane
5. Acetone
24. Pyridine
6. Isopropanol
25. Toluene
7. Acetonitrile
26. Ethylene glycol
8. Methylene chloride
27. Tetrachloroethylene
9. tert-Butanol
28. Formamide
10. MTBE
29. N,N-Dimethylformamide
11. Hexane
30. Chlorobenzene
12. Allyl alcohol
31. Ethylbenzene
13. n-Propanol
32. m-Xylene
14. Methyl ethyl ketone
33. p-Xylene
15. Ethyl acetate
34. 3-Picoline
16. Tetrahydrofuran
35. o-Xylene
17. Chloroform
36. Styrene
18. Cyclohexane
37. Anisole
38. Benzaldehyde
39. N-Methylpyrrolidone
14807
32, 33
35, 36
20
30
31
37
38
29
34
27
25
39
21
24
22
17
App ID 14807
16
19
2
4
5
10
11
26
23
18
14 15
12
28
13
10
15
20
min
Sample:

Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-WAXplus
30 meter x 0.25 mm x 0.25 m
7HG-G013-11
Split 50:1 @ 220 C, 0.2 L
30 C for 1 min to 70 C at 14 C/min to 220 C
15926
52
40
43
48
45,46
53
30,31
6
8
1
2
25
32 35
24
11,12
10
56
17,18
22
51
16
20
19
23
21
13
28 29
27
26
41
34 36
33
39
54
38
50
44
42
37
47
49
Phenomenex
57
App ID 15926
14,15
55
1. Air
2. Pentane
3. Hexane
4. Ethyl ether
5. 2-Methoxy-2-methyl-2propane (MTBE)
6. Heptane
7. Cyclohexane
10. Acetone
11. Formic acid ethyl ester
(Ethyl formate)
12. Acetic acid methyl ester
(Methyl acetate)
13. Tetrahydrofuran (THF)
16. Ethyl acetate
17. Acetic acid-1-methyl
ethyl ester
18. 2-Butanone (MEK)
20. Dichloromethane
21. Isopropanol
22. Benzene
23. Propyl acetate
26. Acetonitrile
27. Methylisobutylketone
(MIBK)
28. Isobutyl acetate

29. Chloroform
30. Toluene
31. n-Propanol
32. Water
33. 1,4-Dioxane
35. Butyl acetate
36. 2-Hexanone (MBK)
37. 2-Methylpropanol
38. Ethylbenzene
39. p-Xylene
40. m-Xylene
41. Butanol
42. Nitromethane
43. Cumene
44. 2-Methoxy ethanol
45. Pyridine
46. o-Xylene
47. 3-Methyl butanol
48. Chlorobenzene
49. n-Pentanol
50. DMF
51. Anisole
52. Tetralin
53. DMSO
54. Ethylene glycol
(1,2-ethanediol)
55. Methylacetamide
56. n-Methylpyrrolidinone
57. Formamide
Solvents
9 min
135
Alcohols
Alcohols
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-624
30 meter x 0.53 mm x 3.00 m
7HK-G005-36
Split 20:1 @ 225 C, 1 L
50 C for 4 min to 130 C @ 14 C/min for 3 min to 200 C
@ 15 C/min for 1.5 min
Sample: All analytes at 16 ng on column except decanol at 32 ng
1. Methanol
12. 3-Pentanol
23. 4-Methylcyclohexanol
2. Ethanol
13. 2-Pentanol
24. Heptanol
3. Isopropanol
14. Pentanol
25. 2-Ethyl-1-Hexanol
4. tert-Butanol
15. 3-Hexanol
26. Octanol
5. Allyl alcohol (2-Propen-1-ol)
16. Cyclopentanol
27. Phenylmethanol
6. Propanol
17. 2-Ethyl-1-Butanol
28. Phenylethanol
7. sec-Butanol
18. Hexanol
29. 2-Phenylethanol
8. Chloroform
19. 4-Heptanol
30. Decanol
9. 2-Methylpropanol
20. Cyclohexanol
31. 3-Phenyl-1-propanol
10. Methallyl alcohol
11. Butanol
7, 8
27
30
21
10
12
14
16
18
19
18
14
20
16
1*
20 min
3
2
10 12
11
22
15
13
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
23 27
28
24
25
10
Zebron ZB-5ms
30 meter x 0.25 mm x 0.50 m
7HG-G010-17
Split 150:1 @ 200 C, 0.2 L
MSD; 20-300 amu
Equal mixture of pure alcohols
1. Methanol
17. n-Hexanol
2. Ethanol
18. Cyclohexanol
3. Isopropanol
19. 4-Heptanol
4. tert-Butanol
5. Allyl alcohol
6. n-Propanol
7. 2-Butanol
23. n-Heptanol
24. 2-Ethylhexanol
25. Benzyl alcohol
10. n-Butanol
26. Phenylethanol
11. 2-Pentanol
27. n-Octanol
12. 3-Pentanol
28. 2-Phenylethanol
13. 1-Pentanol
29. Benzenepropanol
28
14. Cyclopentanol
30. n-Decanol
27
15. 3-Hexanol
26
16. 2-Ethylbutanol
25
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
30
4
11
6
9
21
16
7
8
9
13
17
20
App ID 15427
11,12
22
18
14
10
136
min
19
15
12
29
24
45
11
Zebron ZB-1
30 meter x 0.25 mm x 0.25 m
7HG-G001-11
Split 20:1 @ 250 C,1 L
50 C to 200 C at 6 C/min
FID @ 300 C
200 ppm Hazardous Substance Mix in Methylene Chloride
1. Aniline
2. Benzyl alcohol
3. 2-Methylphenol
9. 2-Nitroaniline
4. 4-Methylphenol
10. 3-Nitroaniline
5. Benzoic acid
11. Dibenzofuran
6. 4-Chloroaniline
12. 4-Nitroaniline
23
40
Hazardous Substances
Alcohols
3334 35 37
36 38 39
32
10
12
14
min
11
10
12
App ID 1266
31
21 26
17
3 4
29 30
26
24
17 1819
20
6
1
25
8*
App ID 14794
22,23
10
11
31
28 29
14
15, 16
App ID 15955
12, 13
Column: Zebron ZB-WAXplus

Injection: Split 40:1 @ 170 C, 0.5 L
Oven Program: 40 C to 75 C @ 6 C/min to 220 C @ 30 C/min for 3 min
Sample: * indicates solvent impurity
1. 1,1-Dichloroethane*
29. 2-Ethyl-1-Hexanol
2. Methanol
16. Pentanol
30. Octanol
3. tert-Butanol
17. 4-Heptanol
31. Decanol
4. Isopropanol
18. Cyclopentanol
32. Phenylethanol
5. Ethanol
19. 2-Ethyl-1-Butanol
33. Undecanol
6. 1,2-Dichloroethylene
20. Hexanol
34. Phenylmethanol
7. Propanol
21. Cyclohexanol
35. 2-Phenylethanol
8. Bromochloromethane* 22. cis-2-Methylcyclohexanol
36. Dodecanol
9. 2-Methylpropanol
23. trans-2-Methylcyclohexanol
37. Tridecanol
10. 3-Pentanol
24. cis-3-Methylcyclohexanol
38. Tetradecanol
11. Allyl alcohol
25. cis-4-Methylcyclohexanol
39. Pentadecanol
(2-Propen-1-ol)
26. Heptanol
40. Hexadecanol
12. 2-Pentanol
13. Butanol
14. 3-Hexanol
22 min
Phenomenex
Phenols
Phenols
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-5ms
30 meter x 0.25 mm x 0.25 m
7HG-G010-11
Split 10:1 @ 250 C,1 L
Sample: Analytes are 20 ppm
1. Phenol
2. 2-Chlorophenol
3. 2-Methylphenol
4. 3-Methylphenol
5. 4-Methylphenol
6. 2-Nitrophenol
15. 4-Nitrophenol
7. 2,4-Dimethylphenol 16. 2,3,4,6-Tetrachlorophenol
8. Benzoic Acid
19. Dinoseb
Zebron ZB-WAXplus
10 meter x 0.10 mm x 0.10 m
7CB-G013-02
Split 15:1 @ 220 C,1 L
60 C to 240 C at 20 C/min for 6 min
MSD
1. 2-Nitrophenol
2. 2-Chlorophenol
3. Phenol
11. Benzoic Acid
4. 2-Methylphenol
5. 4-Methylphenol
7. 3-Methylphenol
5,6
4,5
3,4
7
11
10
13
12
16
19
17
14
13
14
18
15
10
11
1
6
12
8 9
15
10 min
10
11
12
min
Ketones
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-WAX
30 meter x 0.25 mm x 0.25 m
7HG-G007-11
Split 20:1 @ 250 C,1 L
40 C for 6 min to 70C @ 10 C/min to 230 C
Detector: MS @ 280 C
Sample: Analytes are 100-200 ppm in pentane and chloroform
1. Propanal
10. Chloroform
2. Cyclopentane
11. Hexanal
3. Propenal
12. Paraldehyde
4. Butanal
13. Heptanal
5. 2-Methyl-2-Propenal
14. Octanal
6. 2-Methylbutanal
15. Contaminant
7. Ethanol
16. Nonanal
8. Pentanal
17. Decanal
9. 2-Methylpentanal
Zebron ZB-WAX
60 meter x 0.53 mm x 1.00 m
7KK-G007-22
Split 10:1@ 250 C,1 L
50 C to 80 C @ 10 C/min to 230 C @ 25 C/min for 8 min
FID @ 275 C
All compunds are at 100 ppm
1. Solvent Impurity
11. 5-Methyl-2-Hexanone
2. 2-Butanone
12. 3-Heptanone
3. 3-Methyl-2-Butanone
13. 2-Heptanone
4. 2-Pentanone
14. 2-Octanone
5. 3-Pentanone
15. 2-Nonanone
6. 4-Methyl-2-Pentanone 16. 2-Decanone
7. 3-Methyl-2-Pentanone 17. 2-Undecanone
8. 3-Hexanone
18. 2-Dodecanone
9. 2-Methyl-3-Hexanone 19. 2-Tridecanone
10. 2-Hexanone
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Aldehydes
App ID 14955
App ID 15871
17
10
16
15
4,5
16
12
13
17
14
13
18
19
12
13 min
11
4
1
11
9
Phenomenex
14
10
15
12
App ID 14878
14 min
9
8 10
App ID 14852
12
7
6
3
10
11
137
Aliphatic Amines
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Aromatic Amines
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-5
30 meter x 0.25 mm x 0.25 m
7HG-G002-11
Split 15:1 @ 220 C, 1 L
MSD; 50-450 amu
1.58 mg/mL each
1. tert-Butylamine
12. Dibutylamine
2. sec-Butylamine
13. Octylamine
3. Isobutylamine
14. Nonylamine
4. n-Butylamine
15. Dipentylamine
5. Diisopropylamine
16. Tributylamine
6. Triethylamine
17. Decylamine
7. Isopentylamine
18. Contaminant
8. Pentylamine
19. Dihexylamine
9. Hexylamine
10. Cyclohexylamine
21. Contaminant
11. Heptylamine
21
Zebron ZB-5ms
30 meter x 0.25 mm x 0.25 m
7HG-G010-11
Split 100:1@ 220 C,1 L
for 4 min
Sample: Equal mixture of pure components diluted in methanol.
1. Piperidine
2. 2-Methylpiperidine
11. N-Ethylaniline
3. Aniline
4. Benzylamine
13. N,N-Diethylaniline
14. Dibenzylamine
6. N-Methylaniline
7. m-Toluidine
8. o-Toluidine
9. N,N-Dimethylaniline
14
18
19
16
14
4
6
App ID 14792
12
9
13
11
20
11
5
6
10
17
2
10 min
10
11
12 min
Note: Peak 17 is reduced in size because the molecular ion peak is outside the
selected mass scan range.
10
App ID 15429
15
12
13
Organoamines
Industrial Chemicals: Nitrogen Based Compounds
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
30 meter x 0.25 mm x 0.20 m
7HG-G017-10
40 C for 2 min to 260 C @ 15 C/min to 280 C @ 8 C/min for
3 min to 320 C @ 15 C/min for 5 min
Sample: Analytes are 50 ppm in Dichloromethane
1. Pyridine
2. N-Nitrosodimethylamine
3. Aniline
4. N-Nitrosodi-n-propylamine
5. 4-Chloroaniline
6. 2-Nitroaniline
7. 3-Nitroaniline
8. Diphenylamine
9. 4-Nitroaniline
10. Benzidine
11. 3,3-Dichlorobenzidine
16519
Zebron ZB-1
30 meter x 0.53 mm x 3.00 m
7HK-G001-36
Split 10:1 @ 250 C,1 L
FID @ 300 C
100 ppm per analyte in Carbon Disulfide (CS2)
1. Acetonitrile
9. Dimethyl sulfoxide
2. Solvent / Carbon disulfide
10. Hexylamine
3. Diethylamine
11. Dibutylamine
4. 1-Methylpyrrolidine
12. Benzylamine
5. N,N-Dimethylbutylamine
13. Methylbenzylamine
6. Nitropropane
14. Nitrobenzene
7. Pyridine
15. Contaminant
8. Dimethylformamide
16. Tributylamine
16233
2
14
10
16
11
2
13
11
12
6
7
1
2
138
10
12
14
16
18
20 min
10
9
1
4
15
5
6
10
App ID 16233
App ID 16519
9
4
12 min
Phenomenex
petroleum / Fuel
C9 C120 Standard: ASTM Method D 7169

Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Peak
Symmetry (As):
0.99
C9
C10

5 meter x 0.53 mm x 0.09 m
7AK-G026-55
FID @ 450 C
This chromatogram has been baseline subtracted. Sample was ASTM
D2887 calibration mix with POLYWAX 1000 in Carbon Disulfide (CS2).
1. Decane (C10)
7. Dopentacontane (C52)
2. Undecane (C11)
8. Hectane (C100)
3. Dodecane (C12)
6. Pentacontane (C50)
9. Decahectane (C110)
10. Icosahectane (C120)
C11
C120 elutes on oven ramp!
C12
C100
C110
C120
Resolution = 2.55
C40
C44
C52
10
20
Yeates Crude Oil
min
C5 C44: ASTM Method D 2887
C44
C40
C32
C36
C24
C28
C20
C7
C8
C9
C10
C11
C12
C5
C6
19256

10 meter x 0.53 mm x 2.65 m
7CK-G026-35
On-Column @ 38 C,1 L
FID @ 370 C
Sample 0.01 % in (CS2). Chromatogram is baseline subtracted.
1. Pentane (C5)
11. Hexadecane (C16)
2. Hexane (C6)
12. Heptadecane (C17)
3. Heptane (C7)
13. Octadecane (C18)
4. Octane (C8)
14. Eicosane (C20)
5. Nonane (C9)
15. Tetracosane (C24)
6. Decane (C10)
16. Octacosane (C28)
7. Undecane (C11)
17. Dotriacontane (C32)
8. Dodecane (C12)
18. Hexatriacontane (C36)
9. Tetradecane (C14)
10. Pentadecane (C15)
C14
C15
C16
C17
C18
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:

5 meter x 0.53 mm x 0.09 m
7AK-G026-55
On-Column @ 38 C,1 L
FID @ 450 C
Sample is 1 % in (CS2). Chromatogram is baseline subtracted.
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
40
30
App ID 19301
C50
10
Phenomenex
20
30
40
50
min
App ID 19311
App ID 19256
450C
10
20
min
139
petroleum / Fuel
PONA Analysis on Gasoline Fractions
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-1
100 meter x 0.25 mm x 0.50 m
7MG-G001-17
Split 40:1 @ 225 C, 0.2 L
35 C (hold 14 min) to 60 C @ 1.1 C/min (hold 19 min) to 280 C @ 2 C/min (hold 5 min)
FID @ 320 C
Ret. Time (min) Sample Analyte
Ret. Time (min) Sample Analyte
11.72 Pentane
28.15 Heptane
19.32
2,2-Dimethylpentane
35.45
2,3,4-Trimethylpentane
19.55
Methylcyclopentane
35.82
Toluene
19.94
Dimethylpentane
36.05
21.83
1-Methyl-1-Cyclohexene
36.99
2,3-Dimethylhexane
21.94
Benzene
54.12 Ethylbenzene
22.94
Cyclohexane
56.41
m-Xylene
24.00
2-Methylhexane
56.70
p-Xylene
24.18
2,3-Dimethylpentane
61.71
o-Xylene
25.04
3-Methylhexane
81.44
Decane
27.04
100.73
Dodecane
11
15
12
17
8 10
0
18 19
40
20
22
21
20
60
80
100 min
App ID 14804
16
App ID 14800
|
Naphtha
Alkylate
20
40
Fluidized Catalytic Cracking
60
80
100
120 min
Retention Time Markers

8
1
14
22
21
12
140
20
40
60
80
100
min
App ID 14803
App ID 14802
18 20
17 19
20
40
60
80
100
120 min
Phenomenex
petroleum / Fuel
Solvents:
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Aromatic Solvents:
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-WAXplus
30 meter x 0.25 mm x 0.25 m
7HG-G013-11
Split 100:1 @ 250 C, 0.5 L
Helium @ 1.6 mL/min (constant pressure)
35 C hold for 3 min to 95 C @ 20 C/min hold for 8 min
FID @ 260 C
1. Pentane
10. Ethanol
2. Methyl formate
11. 2-Butanol
3. Acetone
12. Toluene
4. Ethyl acetate
13. Propanol
5. Methyl ethyl ketone
14. Ethylbenzene
6. Methanol
15. p-Xylene
16. m-Xylene
8. Methylene chloride
17. 1-Butanol
9. Benzene
18. o-Xylene
1415
12
16
18
12
Zebron ZB-WAX
60 meter x 0.32 mm x 0.50 m
7KM-G007-17
Split 100:1 @ 250 C, 0.1 L
Helium @ 1.7 mL/min (constant pressure)
75 C hold for 10 min to 95 C @ 3 C/min hold for 4 min to 200 C
@ 30 C/min hold for 6 min
Sample: 1. Hexane
11. m-Xylene
21. Pseudocumene
2. Heptane
12. Cumene
22. Cyclohexanone
3. Cyclohexane
13. o-Xylene
23. 1,3-Diethylbenzene
4. Nonane
14. Propylbenzene
5. Benzene
15. p-Ethyltoluene
25. Butylbenzene
6. Toluene
16. m-Ethyltoluene
7. 1,4-Dioxane
17. t-Butylbenzene
27. Phenylacetylene
8. Undecane
18. s-Butylbenzene
28. Durene
9. Ethylbenzene 19. Styrene
29. Phenylacetate
10. p-Xylene
20. Tridecane
27
23
28
9, 10
25
17
11
10
13
29
11 12 13 14 16
15 17
18 19
21
20
22
26
7 min
10
15
20
25
min
Petroleum Oxygenates:
ASTM Method D 4815
Zebron ZB-1
60 meter x 0.25 mm x 1.00 m
7KG-G001-22
Split 40:1 @ 200 C, 0.2 L
40 C for 6 min to 50 C at 5 C/min for 4 min to 175 C
Sample: 1. Methanol
9. Isobutanol
2. Ethanol
10. tert-Butyl ethyl ether
3. Isopropanol
11. tert-Pentanol
4. tert-Butanol
12. Methylcyclopentane
5. 1-Propanol
6. Methy tert-Butyl Ether 14. n-Butyl alcohol
7. sec-Butanol
15. Benzene
8. Isopropyl ether
16. tert-Amyl methyl ether
15
4
2
11
9
Phenomenex
5 min
37
8 min
13
10
11
App ID 14849
12
6
App ID 14821
1
16
14
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Zebron ZB-1
60 meter x 0.32 mm x 3.00 m
7KM-G001-36
Split 10:1 @ 200 C, 5 L
40 C Isothermal
FID @ 200 C
1. Ethane
2. Propane
3. Isobutane
4. Butane
2
Butane Purity

App ID 16141
App ID 16140
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
24
5, 6
15 min
141
petroleum / Fuel
Extended Natural Gas:
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Impurities in Ethylbenzene:
ASTM Method D 5060
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-1
60 meter x 0.32 mm x 1.00 m
7KM-G001-22
Split 50:1 @ 250 C, 500 L
35 C hold for 3 min to 200 C ramp @ 30 C/min
FID @ 250 C
1. Methane
5. Butane
2. Ethane
6. Isopentane
3. Propane
7. Pentane
4. Isobutane
8. Heptane
16146
Zebron ZB-FFAP
50 meter x 0.32 mm x 0.50 m
7JM-G009-17
Split 200:1 @ 160 C, 0.5 L
70 C hold for 8 min to 200 C ramp @ 20 C/min hold for 6 min
FID @ 240 C
1. Aliphatic Hydrocarbons
6. m-Xylene
2. Benzene
7. Cumene
3. Toluene
8. o-Xylene
4. Ethylbenzene
9. Propylbenzene
5. p-Xylene
10. Styrene
4
3
1
7
9
4.5
5.5
6.0
Methyl-t-Butyl Ether (MTBE):

Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
10
8
min
6.5
6
5
App ID 16145
App ID 16146
min
Denatured Bioethanol:
ASTM Method D 5501
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Instrument:
Sample:
Zebron ZB-1
100 meter x 0.25 mm x 0.50 m
7MG-G001-17
Split 50:1 @ 200 C, 1 L
FID @ 250 C
1. Methanol
8. 2-Methyl-2-butene
2. Contaminant
9. Methyl-t-butyl ether
3. 2-Methylbutane
10. tert-Butyl ethyl ether
4. Pentane
11. tert-Amyl methyl ether
5. trans-2-Pentene
12. 2,4,4-Trimethyl-1-pentene
6. t-Butanol
13. 4,4-Dimethyl-2-neopentyl-1-pentene
7. cis-2-Pentene
14. 2,2,4,6,6-Pentamethyl-3-heptene (cis, trans)
Zebron ZB-Bioethanol
15 meter x 0.25 mm x 1.00 m
7EG-G020-22
Split 50:1 @ 300 C, 1 L
Hydrogen @ 25 cm/sec (constant flow)
55 C for 1.7 min to 260 C @ 40 C/min (hold 2.67 min)
FID @ 300 C
Shimadzu GC-2010 with a AOC-20i Automatic Liquid Handler
1. Methanol
2. Ethanol
3. Denaturant
16668
13
3
2
12
56
7
34 8
11
App ID 16245
2
1
142
10
12
14
16
18
20 min
App ID 16668
10
5 min
Analysis!
14
min
Phenomenex
petroleum / Fuel
ASTM Method D 3710 - Fast
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Sulfur Components in Fuel
16212
15
16
18
13
17
Zebron ZB-1MS
30 meter x 0.32 mm x 1.00 m
7HM-G011-22
Split 10:1 @ 270 C, 1 L
SCD @ 320 C
California Phase II Reference Fuel - 30 mg/kg total sulfur
1. Thiophene
2. Dimethyldisulfide
3. 2-Me-thiophene
4. 3-Me-thiophene
5. C2-Thiophenes
6. Diethylsulfide
7. Dibutylsulfide
8. Benzothiophene
9. C1-Benzothiophenes
14
12
3 4
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
Zebron ZB-1
15 meter x 0.53 mm x 0.50 m
7EK-G001-17
Split 50:1 @ 200 C, 0.1 L
Helium @ 7 mL/min (constant pressure)
FID @ 220 C
1. Isopentane
10. p-Xylene
2. Pentane
11. o-Xylene
3. 2-Methylpentane
12. Propylbenzene
4. Hexane
13. Decane
5. 2,4-Dimethylpentane
14. Butylbenzene
6. Heptane
15. Dodecane
7. Toluene
16. Tridecane
8. Octane
17. Tetradecane
9. m-Xylene
18. Pentadecane
16503
9,10
1
11
min
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:

16211
10
15
20min
Free and Total Glycerin in B-100 Biodiesel

Methyl Esters: ASTM Method D 6584
App ID 16503
App ID 16212
3
Free and Total Glycerin in Biodiesel:

Method EN 14105
Column:
Dimensions:
Part No.:
Injection:
Carrier Gas:
Oven Program:

15 meter x 0.32 mm x 0.10 m
7EM-G015-02
FID @ 380 C
A 2.0 m x 0.53 mm guard column was connected to the analytical
column per ASTM method requirements
1. Glycerol
6. Tricarpin (IS 2)
2. Butanetriol (IS 1)
7. Diglycerides
3. Ester
8. 1,3-Diolein
4. Monoglycerides
9. Triglycerides
5. 1-Monooleoyl-rac-glycerol
10. Triolein

15 meter x 0.32 mm x 0.10 m
7EM-G015-02
Helium @ 5.79 cm/sec (constant flow)
@ 7 C/min to 370 C @ 10 C/min (hold 5 min)
Sample: A 5 m x 0.53 mm guard column was connected to the
analytical column
1. Glycerol
4. Tricarpin (IS 2)
2. Butanetriol (IS 1)
5. Diolein
3. Monoolein
6. Triolein
16669
3
6
2
7
10
8
Phenomenex
10
20
App ID 16211
min
6
5
10
20
30
App ID 16669
min
143
HPLC Column Selection
Develosil................................................................................................ 164
Column Selection Tree........................................................................... 146
EnviroSep.............................................................................................. 166
Column Selection by Application.......................................................... 147
EZ:faast.................................................................................................. 278
Column Selection by Manufacturer................................................ 148-150
Gemini / Gemini-NX.......................................................................... 168
Column Selection by Separation Mode................................................. 151
Hamilton................................................................................................ 176
Column Selection by USP Specifications....................................... 152-153
Hypercarb...................................................................................... 179, 317
Column Selection by EP Listing..................................................... 154-156
HyperClone............................................................................................ 177
HPLC Column Protection
Hypersil.............................................................................................. 179
Column Performance Check Standards . ...................................... 365-366
HypersiL BDS..................................................................................... 179
Presaturator Guard Columns................................................................. 355
IB-Sil...................................................................................................... 179
SecurityGuard Cartridge System........................................................... 235
Inertsil.................................................................................................... 179
System Test Kit...................................................................................... 355
Jupiter.................................................................................................. 296
Kinetex................................................................................................. 180
HPLC Columns

Aeris..................................................................................................... 283
Kromasil................................................................................................. 191
Aqua...................................................................................................... 157
LiChrosorb..................................................................................... 192, 209
Asahipak................................................................................................ 158
LiChrospher........................................................................... 192, 208, 209
Axia Packed Columns....................................................................... 336
Luna...................................................................................................... 193
BioSep.................................................................................................. 292
Lux........................................................................................................ 318
Bondclone............................................................................................. 160
Maxsil..................................................................................................... 208
Capcell Pak............................................................................................ 161
Merck KGaA.......................................................................................... 208
Ceramospher and Chiral CD-Ph............................................................ 313
Merck KGaA Chiral................................................................................ 324
Chiral Method Development Kits........................................................... 316
Nucleosil................................................................................................ 210
Chirex................................................................................................... 314
Nucleosil Chiral...................................................................................... 324
Chromolith............................................................................................. 163
Onyx...................................................................................................... 211
Clarity..................................................................................................... 301
Partisil............................................................................................ 215, 263
Columbus.............................................................................................. 163
PartiSphere............................................................................................ 263
Cosmosil................................................................................................ 163
Phenogel............................................................................................. 216
Curosil.................................................................................................... 163
PhenoSphere......................................................................................... 222
144
Phenomenex
HPLC
Essentials for
Improved HPLC Reproducibility:
SecurityGuard,
p. 235
ThermaSphere TS-130,
p. 357
Column Check Standards,
pp. 365-366
PhenoSphere-NEXT............................................................................... 222
Polymer Labs......................................................................................... 223
PolymerX............................................................................................. 224
PolySep-GFC-P..................................................................................... 225
PREP/Preparative.................................................................................. 335
PrimeSphere.......................................................................................... 225
Prodigy................................................................................................... 226
Rezex.................................................................................................... 228
SecurityGuard.................................................................................... 235
SecurityGuard ULTRA............................................................... 239, 276
Selectosil............................................................................................... 240
Shodex................................................................................................... 240
Shodex Chiral ....................................................................................... 325
SphereClone.......................................................................................... 246
Spherex.................................................................................................. 247
Spherisorb............................................................................................. 247
STAR-ION A300..................................................................................... 248
Sumichiral OA Chiral.............................................................................. 326
Synergi................................................................................................. 249
Tosoh Bioscience LLC........................................................................... 260
Ultracarb................................................................................................ 262
Ultrasphere............................................................................................ 262
Ultremex................................................................................................ 262
Ultron ES Chiral..................................................................................... 325
Vydac..................................................................................................... 262
Whatman............................................................................................... 263
ZORBAX................................................................................................ 264
HPLC Accessories and Consumables............................... 353-378
HPLC
LIQUID CHROMOATOGRAPHY
144 - 264
HPLC Column Selection Tree

Sample
MW
Sample
Solubility
Separation
Mode
HexaneSoluble
OrganicSoluble
Methanol
Methanol/H20
Soluble
THF-Soluble
Our Recommended
Column
Normal Phase
Adsorption
Kinetex HILIC
Luna Silica(2)
Onyx Silica
180
193
211
Normal Phase
Bonded
Luna CN, NH2, HILIC
193
Reversed Phase
Bonded
Chiral
Kinetex C18, XB-C18, C8

Synergi Max-RP, Fusion-RP
Luna C8(2), C18(2)
Gemini C18, Gemini-NX C18
Lux
180
249
193
168
318
Gel Permeation
GPC
Phenogel 50 , 100
216
Kinetex C18, XB-C18, C8, PFP, Phenyl-Hexyl

Synergi Polar-RP, Hydro-RP
Luna C8(2), C18(2), Luna PFP(2)
Onyx C8, C18
180
249
193
168
211
Lux
Luna HILIC
Kinetex C18, XB-C18, C8
Synergi Max-RP, Hydro-RP
Luna C8(2), C18(2)
Onyx C8, C18
318
193
180
249
193
168
211
Ion-Exchange
Luna SCX, NH2

PhenoSphere SAX
193
222
HILIC
Kinetex HILIC
Luna HILIC
180
193
Lux
Chirex
318
314
Jupiter Proteo
Gemini C18
Aeris PEPTIDE
296
168
283
Unknown
MW Range
Phenogel Linear (2)

Shodex GPC
220
240
Known
MW Range
Specific Pore: Phenogel

Shodex GPC
216
240
pH 2-7.5
BioSep-SEC-S Series
292
pH > 7.5
PolySep-GFC-P
225
CationExchange
Shodex IEC CM-825
240
AnionExchange
Shodex IEC DEAE

Clarity Oligo-WAX
240
301
pH 29
Aeris WIDEPORE C4, XB-C8, XB-C18

Jupiter 300 C4, C5, C18
283
296
pH > 9
Hamilton PRP-3
176
Hydrophobic
Interaction (HIC)
Shodex HIC
240
Affinity/Bioaffinity
Shodex AFpak
240
MW<5000
Reversed Phase
Non ionic
Chiral
HILIC
AqueousSoluble
Ion Pairing / Reversed Phase

Ionic
HPLC Column Selection Tree | HPLC
Estimate MW of Sample
Chiral
Peptides
OrganicSoluble
Reversed Phase
Gel Permeation
Chromatography
(GPC)
Gel Filtration
Aqueous
GFC/SEC
MW>5000
Ion-Exchange
AqueousSoluble
Reversed Phase
146
Page
Phenomenex
HPLC Column Selection by Application

This table is to aid you in selecting the right column for your application. For application notes or method development assistance
please call your technical representative.
Amino Acids
Phenomenex EZ:faast (GC and LC/MS)
Phenomenex Chirex (chiral)
Phenomenex Lux (chiral)
Page
278
314
318
Anions
Phenomenex STAR-ION A300
Phenomenex PhenoSphere SAX
Phenomenex Luna NH2
Hamilton PRP
Shodex IC
248
318
222
193
176
240
Antibiotics
Phenomenex Gemini / Gemini-NX
Phenomenex Kinetex
Phenomenex Luna
Phenomenex Synergi
168
180
193
249
Biotechnology/Life Sciences
Phenomenex Aeris WIDEPORE
Phenomenex Aeris PEPTIDE
Phenomenex Clarity
Phenomenex Jupiter 300/Jupiter Proteo
Phenomenex Onyx
Phenomenex BioSep-SEC-S
Phenomenex PolySep-GFC-P
Phenomenex Luna SCX
Phenomenex Luna NH2
Shodex GFC, KW, AFpak
193
176
Enantiomers (Chiral)
Phenomenex Chirex
Phenomenex Lux
Macherey-Nagel Nucleodex
Shiseido Chiral CD-Ph
314
318
324
313
Environmental (Carbamates, PAHs, Explosives)

Phenomenex EnviroSep
Phenomenex Kinetex
Phenomenex Luna
Phenomenex Synergi
Phenomenex Zebron (GC)
166
168
180
193
249
61
Foods, Flavors and Fragrances

Phenomenex Rezex
Phenomenex Kinetex
Phenomenex Luna
Phenomenex Onyx
Phenomenex Synergi
Phenomenex
Page
283
301
168
193
249
222
Organic Acids
Phenomenex Synergi Hydro-RP
Phenomenex Rezex
249
228
Peptides/Proteins
Phenomenex Aeris PEPTIDE
Phenomenex Jupiter 300/Jupiter Proteo
Phenomenex Luna SCX, NH2
Phenomenex BioSep-SEC-S
283
283
296
193
292
228
168
180
193
318
211
249
61

Phenomenex Kinetex
Phenomenex Synergi
Phenomenex Luna
Phenomenex Onyx
Phenomenex EnviroSep-PP
168
180
249
193
318
211
166
61
Pharmaceuticals
Phenomenex Kinetex
Phenomenex Synergi
Phenomenex Luna
Phenomenex Onyx
Phenomenex Chirex (chiral)
168
180
249
193
318
211
314
Polymers, Plastics, Rubber

Phenomenex Phenogel
Shodex Asahipak GF
61
216
158
Vitamins
Phenomenex Kinetex
Phenomenex Synergi
Phenomenex Luna
Phenomenex Onyx
168
180
249
193
211
Taxanes
Phenomenex Luna PFP(2)
193
Textiles/Dyes
Phenomenex Kinetex
Phenomenex Synergi
Phenomenex Luna
Phenomenex Phenogel GPC
HPLC | HPLC Column Selection by Application
193
228
240
Cations
Phenomenex Luna SCX
Hamilton PRP

Phenomenex Clarity
Phenomenex Gemini C18
Phenomenex Luna NH2, SCX
Phenomenex Synergi Polar-RP
Pesticides, Herbicides, and Dioxins

283
283
301
296
211
292
225
193
193
240
Carbohydrates
Phenomenex Luna NH2
Phenomenex Rezex
Shodex Sugar
Nucleosides and Nucleotides
168
180
249
193
216
147
HPLC Column Selection by Manufacturer

In recognizing the tremendous difficulty the chromatographer has
in choosing from literally hundreds of columns and to aid in your
selection of alternative materials from different manufacturers, an
HPLC column selection guide is presented below.
This selection is, neither in terms of manufacturers nor in terms of
their products, a complete list, and the accuracy of the data is not
guaranteed.
Column
Phenomenex
Alternative*
Phenomenex Recommended
Alternative**
BioSep-SEC-S
Aeris WIDEPORE
Kinetex XB-C18
Luna
Gemini
Kinetex
Luna 10 m
HyperClone
Kinetex XB-C18
Jupiter 300
Synergi Hydro-RP
BioSep-SEC-S
Gemini-NX C18
Jupiter 300
Synergi Fusion-RP
Clarity Oligo-RP
Luna NH2
BioSep-SEC-S
Jupiter 300
Kinetex C18
Synergi
Luna
Synergi 2.5 m
Luna 10 m PREP
Luna
Luna
Aeris WIDEPORE
Synergi Hydro-RP
BioSep-SEC-S
Gemini C18
Aeris WIDEPORE
Synergi Hydro-RP
Clarity Oligo-MS
Rezex
Luna
Ultracarb ODS (20)
Luna
Jupiter 300
SphereClone
Luna
Luna
Synergi Hydro-RP
Luna Phenyl-Hexyl
Bondclone
Luna
Luna
HyperClone
Rezex
Econexsphere (Inquire)
Luna C18(2)
Jupiter 300
Jupiter 300
Luna
Synergi Hydro-RP
BioSep-SEC-S
Jupiter
Synergi Hydro-RP
Luna
Jupiter 300
EnviroSep PP
Jupiter 300 C18
Jupiter 300
Jupiter 300
Prodigy (3)
Jupiter 300 C18
Jupiter 300 C4
Kinetex
Aeris WIDEPORE
Synergi Max-RP
Luna C18(2)
Synergi Max-RP
Aeris WIDEPORE
Synergi Hydro-RP
Gemini-NX C18
Kinetex
Synergi Fusion-RP
Kinetex Phenyl-Hexyl
Synergi Hydro-RP
Synergi Max-RP
Kinetex
Luna
Rezex
Chirex
Synergi Hydro-RP
Kinetex
Aeris WIDEPORE
Aeris WIDEPORE
Gemini
Synergi Fusion-RP
BioSep-SEC-S
Aeris WIDEPORE
Synergi Fusion-RP
Kinetex
Aeris WIDEPORE
Shodex AFpak
Synergi Hydro-RP
Aeris WIDEPORE
Hamilton PRP-3
Aeris WIDEPORE
Shodex IEC
Aeris WIDEPORE
Luna
Aeris WIDEPORE XB-C18
Aeris WIDEPORE C4
Gemini
Aeris WIDEPORE
HPLC Column Selection by Manufacturer | HPLC
Alltech/Vydac/Jones/Grom (Grace Davison Discovery Sciences)
148
Adsorbosphere
Adsorbosphere HS
Adsorbosphere XL
Adsorbosphere XL 300
Allsphere
Alltima
Alltima HP
Alltima AQ
Alltima Phenyl
Alphabond
Apex
Apollo
Brava
Carbohydrate
Chirobiotic
Econosphere
Denali
Everest
Genesis 300
Genesis
Genesis AQ
Macrosphere GPC
Macrosphere 300
Platinum/Platinum EPS100
Prevail
Prosphere 300
Venture
Vydac 202TP (PAH)
Vydac 228 TP
Vydac 259VHP
Vydac 238TP (C8, C18)
Vydac 300/301VHP
Vydac 201TP
Vydac 201/208SP
Vydac 218TP/218MS/238MS
Vydac 214TP/214MS
Vydac 208TP
Vision HT
Vydac ProZAP
Phenomenex
Alternative*
Alternative**
Rezex
BioSep-SEC-S
Luna C18(2)
Jupiter 300
Rezex
BioSep-SEC-S
Kinetex
Shodex IEC
Aeris WIDEPORE
Shodex IEC
Shodex IEC
Bio-Rad
Bio SEC
Poroshell 300
Poroshell 120 C18
ZORBAX Eclipse-XDB
ZORBAX Eclipse Plus
ZORBAX Rapid Resolution HT
ZORBAX PrepHT
ZORBAX Rx
ZORBAX SB 80
ZORBAX SB 300
ZORBAX SB Aq
ZORBAX GF (BioSeries)
ZORBAX Extend-C18
ZORBAX 300 Extend
ZORBAX Bonus RP
ZORBAX Oligo
ZORBAX Carbohydrate
Column
Aminex
Bio-Sil SEC
Bio-Sil C18
Bio-Sil Rex
Hi-Pore
Macro-Prep
UNOsphere
Chiral Technologies/DAICEL Chemical Industries, Ltd.

CHIRALCEL AY-H
CHIRALCEL OD-H
CHIRALCEL OJ-H
CHIRALCEL OZ-H
CHIRALPAK AD-H
CROWNPAK
Lux Amylose-2
Lux Cellulose-1
Lux Cellulose-3
Lux Cellulose-2
Lux Cellulose-2
Lux Cellulose-2
Lux Cellulose-4
Lux Cellulose-4
Lux Amylose-2
Nucleodex
Dionex (Thermo Fisher Scientific)

Acclaim 120
Acclaim 300
Acclaim PA
Acclaim PA 2
Acclaim OA
Acclaim Surfactant
AminoPac PA
CarboPac (MA, PA)
DNAPac
Ionpac AS series
IonPac CS series
IonPac ICE AS series
OmniPac
ProPac
Luna
Jupiter
Synergi Fusion-RP
Synergi Fusion-RP
Synergi Hydro-RP
Asahipak IEC
STAR-ION A300
Shodex IC series
Rezex ROA
Kinetex
Aeris WIDEPORE
Synergi Hydro-RP
Gemini-NX C18
Synergi Fusion-RP
Gemini
Asahipak IEC/ES
Rezex
Clarity Oligo-WAX
Shodex IC series
Hamilton PRP-X200
Rezex ROA
Luna SCX
Shodex IEC
Synergi Fusion-RP
Nucleosil
Ultracarb ODS (30)
Synergi Fusion-RP
Luna
Synergi 2.5 m
Luna Phenyl-Hexyl
PhenoSphere C1
Luna C8(2), C18(2)
Synergi Fusion-RP
Synergi Hydro-RP
Luna
Synergi Hydro-RP
Synergi Hydro-RP
Gemini
Kinetex
Kinetex C8
Develosil TMS-UG (C1)
Kinetex C8, C18
Asahipak ODS-50
Shodex IEC CM-825
Asahipak ES-502C 7C
Synergi Hydro-RP
Luna 10m PREP C18(2)

Prodigy ODS(2)
Prodigy ODS(3)
Jupiter C5
Luna 10m C18(2)

Luna C18(2)
Luna C18(2)
Aeris WIDEPORE C8
E.S. Industries
Aquasep
Chromegabond
Chromegabond HC
Chromegabond BAS
Chromegabond WR
Epic
FluoroSep-RP Phenyl
FluoroSep-RP Octyl
Gammabond C1
Gammabond C8, C18
Gammabond PVP
Gammabond SCX
Gammabond WCX
Protec-RP
GL Sciences
Inertsil ODS-Prep-100
Inertsil ODS(2)
Inertsil ODS(3)
Inertsil 300 WP300 C8
* Alternative - This category indicates an alternative column which will

likely give a similar selectivity.
** Recommended Alternative - This category indicates an alternative
column which may yield somewhat different selectivity but may also
lead to improved resolution.
Phenomenex

This selection is, neither in terms of manufacturers nor in terms
of their products, a complete list, and the accuracy of the data
is not guaranteed.
Column
Phenomenex
Alternative*
Alternative**
MAC-MOD/Bischoff/ACT/Advanced Materials Technology

ACE
ACE-AQ
ACE-300 A
HALO
HALO Peptide ES-C18
Mac-Mod ACE
Mac-Mod Hydrobond
Mac-Mod ACE-AQ
Mac-Mod ACE-300
ProntoSIL 120
ProntoSIL 300
ProntoSIL Aq 120
ProntoSIL Aq PLUS
ProntoSIL SH 120
ProntoSIL ACE-EPS
ProntoSIL Chiral AX
ProntoSIL C30
Kinetex XB-C18
Kinetex XB-C18
Aeris WIDEPORE
Luna HST
Aeris PEPTIDE XB-C18
Kinetex C18
Synergi Hydro-RP
Synergi Hydro-RP
Aeris WIDEPORE
Kinetex
Aeris WIDEPORE
Develosil RP-Aqueous(C30)
Synergi Fusion-RP
Luna C18(2)
Aqua 200
Chirex
Luna Phenyl-Hexyl
Ultracarb ODS (30)

Develosil ODS-UG
Synergi Hydro-RP
HyperClone
Ultracarb ODS (20)
HyperClone BDS
Synergi Hydro-RP
Kinetex
Aeris WIDEPORE
Luna C18(2)
Luna C18(2)
Gemini-NX C18
Luna C18(2)
Luna C18(2)
Luna C18(2)
Synergi Fusion-RP
Synergi
Jupiter
Restek
Allure
Allure Acidix
Allure Basix
Pinnacle DB
Pinnacle Ultra C18
Pinnacle II
Ultra Aqueous
Ultra II
Viva
Supelco / Sigma-Aldrich Biotechnology LP

Ascentis
Ascentis Express
Ascentis Peptide
Discovery Bio
Discovery HSF5
Discovery HSC18
Discovery C18
Discovery HS PEG
Discovery RP C16 Amide
Discovery (C18, C16)
Hisep
Supelco ABZ, ABZ+
Supelco LC-18-T
Supelco LC-18-S
Supelco LC-F
Supelco LC-PAH
Supelcosil LC
Supelcosil LC-DB
Supelcogel
Supelcogel ODP-50
Supelcosil LC-304/308/318
Supelcosil LC-NH2-NP
Supelcosil LC-PCN
Supelcosil LC-SAX
Supelcosil LC-SCX
Suplex pKb-100
Phenomenex
Synergi
Kinetex
Aeris WIDEPORE
Jupiter 300
Luna PFP(2)
Luna C18(2)
Luna C18(2)
Nucleosil Diol
Synergi Fusion-RP
Synergi Hydro-RP
Capcell Pak MF
Luna C8(2)
Prodigy (3)
Prodigy (3)
Luna PFP(2)
EnviroSep PP
PhenoSphere-NEXT
HyperClone BDS
Rezex
Asahipak ODP-50
Jupiter 300
Selectosil NH2
Luna CN, Capcell UG-CN
PhenoSphere SAX
PhenoSphere SCX
Gemini-NX C18
Luna HST
Aeris PEPTIDE
Aeris WIDEPORE
Kinetex PFP
Kinetex C18
Kinetex C18
Synergi Fusion-RP
Luna C18(2)
Asahipak GS-320
Luna C18(2)
Luna C18(2)
Luna C18(2)
Synergi Polar-RP
Synergi Hydro-RP
Synergi Hydro-RP
Synergi Hydro-RP
Rezex
Luna C18(2)
Aeris WIDEPORE
Luna NH2
Develosil CN-UG
Luna SCX
Luna C8(2)
Phenomenex
Alternative*
Alternative**
Kinetex
Synergi Hydro-RP
Luna
BioSep-SEC-S
Shodex IEC
Jupiter 300
Prodigy (3)
Luna
Luna Phenyl-Hexyl
Synergi Fusion-RP
Luna C18(2)
Luna PFP(2)
Luna
EnviroSep PP
HyperClone
Luna
Synergi Fusion-RP
Synergi Fusion-RP
Synergi Hydro-RP
Luna
Develosil ODS-MG
Kinetex
BioSep-SEC-S
Clarity Oligo-WAX
Aeris WIDEPORE
Luna
Gemini
Synergi Hydro-RP
Synergi Max-RP
Kinetex PFP
Gemini
Kinetex
Synergi Hydro-RP
Synergi Max-RP
Synergi Max-RP
Synergi Hydro-RP
Synergi Hydro-RP
Synergi Max-RP
Thermo Fisher Scientific

Accucore
Aquasil
BetaBasic
BioBasic SEC
BioBasic IEX
BioBasic RP
BETASIL
BetaMax
BETASIL Phenyl-Hexyl
Carbamate
Deltabond
Fluophase
Hypercarb
Hypersil GOLD
Hypersil Green
Hypersil
HyPURITY
HyPURITY ADVANCE
HyPURITY AQUASTAR
Prism RP
Varian / Polymer Labs (Agilent Technologies)

Metacarb
MetaChiral
MetaSil
MetaSil AQ C18 120A, 200
Microsorb
Microsorb 300
PL-Aquagel-OH
PLgel
PL Hi-PLEX
PLRP-S
PLRP-S 300
PlusPore
Polaris C18 Amide, C8 Ether
Pursuit
Pursuit DiPhenyl
Taxsil (1, 2, 3)
Chirex
Prodigy
Aqua C18 125A, 200
Luna
Jupiter 300
PolySep GFC-P
Phenogel
Rezex
PolymerX RP-1
Hamilton PRP-3
Phenogel
Luna
Luna PFP(2)
Rezex
Lux
Luna
Synergi Hydro-RP
Synergi
Aeris WIDEPORE
Shodex OHpak SB-800H
Phenogel
Rezex
Gemini-NX C18
Aeris WIDEPORE
Phenogel
Synergi Fusion-RP
Synergi
Gemini C6-Phenyl
Kinetex PFP
HPLC | HPLC Column Selection by Manufacturer
Gemini-NX C18
Synergi Fusion-RP
Jupiter 300
Kinetex
Gemini-NX C18
Synergi Fusion-RP
Develosil RP-Aqueous
Jupiter 300
Luna C18(2)
Jupiter 300
Synergi Hydro-RP
Synergi Hydro-RP
Gemini-NX C18
Synergi Hydro-RP
Develosil C30
Column

149

This selection is, neither in terms of manufacturers nor in terms of
their products, a complete list, and the accuracy of the data is not
guaranteed.
Column
Phenomenex
Alternative*
Alternative**
Luna HST
Kinetex XB-C18
Synergi Fusion-RP
Shodex IEC
BioSep-SEC-S
EnviroSep CM
PhenoSphere NH2
PrimeSphere
Jupiter 300
EnviroSep ABC
Hamilton PRP-X100
Bondclone
Bondclone Silica
Phenogel
Clarity Oligo-RP
Shodex IEC
Shodex AFpak
BioSep-SEC-S
Aeris WIDEPORE
Aeris WIDEPORE
PhenoSphere
SphereClone
Rezex
Luna
Luna C18(2), C8(2)
Synergi Fusion-RP
Phenogel
Phenogel
PolySep-GFC-P
Gemini-NX C18
Kinetex
Gemini-NX
Gemini-NX
Synergi 2.5 m
Kinetex
Synergi Hydro-RP
BioSep-SEC-S
Jupiter 300
Synergi Hydro-RP
Luna NH2
Luna
Aeris WIDEPORE
Luna NH2
STAR-ION A300
Synergi Hydro-RP
Luna Silica
Phenogel
Synergi Hydro-RP
Clarity Oligo-MS
BioSep-SEC-S
Jupiter 3m C18
Aeris PEPTIDE
Luna
Synergi Hydro-RP
Rezex
Gemini-NX
Synergi Max-RP
Synergi Hydro-RP
Phenogel
Phenogel
Shodex OHpak SB
Synergi Max-RP
Luna
Gemini
Gemini
HPLC Column Selection by Manufacturer | HPLC
Waters
ACQUITY BEH
ACQUITY CSH
Atlantis
BioSuite IEX
BioSuite SEC
BioSuite RPC
Carbamate
Carbohydrate
Deltapak 100A
Deltapak 300A
Envirogel
GST
IC-pak
Bondapak
Porasil
Styragel
Novapak 4 m
OST
Protein-Pak IEC
Protein-Pak Affinity
Protein-Pak SW
PrST
PST
Resolve
Spherisorb
Sugar-pak
SunFire
Symmetry C18, C8
Symmetry Shield C18, C8
Styragel
UltraStyragel
Ultrahydrogel
XBridge
XSelect
XTerra MS
XTerra RP

150
Phenomenex
HPLC Column Selection by Separation Mode

This table is to aid you in selecting the right column from Phenomenex
for the separation mode you desire. For specific application notes
or method development assistance please call your Phenomenex
technical consultant.
Separation Mode
Adsorption Chromatography
Phenomenex Kinetex HILIC
Phenomenex Luna Silica
Phenomenex Onyx Silica
Page
180
193
211
Affinity Chromatography
Shodex AFpak
240
Chiral Chromatography
Phenomenex Chirex
Phenomenex Lux
Hypersil Hypercarb
Macherey-Nagel Nucleosil Chiral, Nucleodex, Resolvosil
Merck ChiraDex
Regis Whelk-O 1
Shinwa Ultron ES
Shiseido Ceramospher and Chiral CD-Ph
Shodex ORpak
Sumika Sumichiral OA
314
318
317
324
324
324
325
313
325
326
292
225
158
240
260
Gel Permeation Chromatography

216
158
240
Hydrophilic Interaction Chromatography

Phenomenex Kinetex Hilic
Phenomenex Luna HILIC
180
193
Hydrophobic Interaction Chromatography

Macherey-Nagel Nucleosil
Shodex HIC
210
240
Ion-Exclusion Chromatography
Phenomenex Rezex
Shodex RSpak, SUGAR
228
240
Ion-Exchange Chromatography
Phenomenex Clarity Oligo WAX
Phenomenex Luna SCX, Luna NH2
Phenomenex Rezex
Hamilton HC, RCX
Macherey-Nagel Nucleosil SAX, SCX
Shiseido Capcell UG-SCX
Shodex Asahipak ES
Shodex IEC, AXpak
Shodex RSpak
Whatman Partisil, PartiSphere SAX, SCX, PAC
301
193
222
228
176
210
161
158
240
240
215, 263
Ion Chromatography
Phenomenex STAR-ION A300
Hamilton PRP
Shodex IC
248
176
240
Ligand Exchange Chromatography

Phenomenex Rezex
Shodex Sugar
Phenomenex
Page
193
158
161
215, 263
Normal Phase Chromatography

Phenomenex Kinetex Hilic
Phenomenex Luna CN, NH2, Silica
Phenomenex Onyx Silica
Phenomenex Prodigy
Agilent Technologies ZORBAX
Merck KGaA LiChrosorb, LiChrospher
Nomura Chemical Develosil
Shodex Asahipak
Whatman Partisil, PartiSphere
180
193
211
226
264
192, 208, 209
210
164
158
215, 263
Partition and Adsorption Chromatography

240
Reversed Phase Chromatography

Phenomenex Aeris
Phenomenex Bondclone
Phenomenex Clarity
Phenomenex EnviroSep
Phenomenex Gemini
Phenomenex Gemini-NX
Phenomenex HyperClone
Phenomenex Jupiter
Phenomenex Kinetex
Phenomenex Luna
Phenomenex Onyx
Phenomenex PhenoSphere-NEXT
Phenomenex PolymerX
Phenomenex Prodigy
Phenomenex SphereClone
Phenomenex Synergi Fusion-RP
Phenomenex Synergi Hydro-RP
Phenomenex Synergi Max-RP
Phenomenex Synergi Polar-RP
Agilent Technologies ZORBAX StableBond, Rx, XDB
Eka Chemicals Kromasil
GL Sciences Inertsil
Hamilton PRP
Hypersil ODS, BDS
Merck KGaA LiChrosorb, LiChrospher, Superspher
Nacalai Tesque Cosmosil
Nomura Chemical Develosil UG series
Shiseido Capcell AG, SG, UG, MG, ACR, AQ
Shodex Asahipak ODP, C8P, C4P
Shodex RSpak
Tosoh Bioscience TSKgel
Waters Spherisorb
Whatman Partisil, PartiSphere
283
160
301
166
168
168
177
296
180
193
211
222
224
226
246
249
249
249
249
264
191
179
176
179
210
192, 208, 209
163
164
161
158
240
260
247
215, 263
HPLC | HPLC Column Selection by Separation Mode
Phenomenex Phenogel
Shodex Asahipak GF
Shodex GPC K, KF, KD, HFIP, HT, UT
Phenomenex Luna SCX

Shodex Asahipak GS
Shiseido Capcell MF
Whatman Partisil
Shodex Sugar
Gel Filtration Chromatography

Phenomenex BioSep SEC/GFC (silica)
Phenomenex PolySep GFC-P (polymer)
Asahipak GF and GS
Shodex GFC OHpak SB, Sugar KS, Protein KW
Tosoh Bioscience TSKgel
Separation Mode
Multi-Mode Chromatography
228
240
151
HPLC Column Selection by USP Listing

For each United States Pharmacopeia (USP) column specification,
you will find listed the most suitable Phenomenex column.
It is widely understood that all HPLC packings are not alike, and no
single column can perform a myriad of desired separations. HPLC
packings differ in hydrophobicity, surface coverage, surface area,
pore size and particle shape.

L1
L2
L3
L4
L5
L6
L7
HPLC Column Selection by USP Listing | HPLC
L8
L9
L10
L11
L12
L13
L14
L15
L16
L17
L18
L19
L20
L21
L22
L23
L24
L25
L26
L27
L28
L29
L30
L31
152
The USP does give chromatographers the flexibility to make adjustments to Monographs. As you can read below, column manufacturers or sources and materials stated in USP Monographs are only
recommendations. Chromatographers can and should change and
adapt the Monographs specifications to yield the most satisfactory
analytical results.
Recommended Phenomenex
Column
Particle Shape
Page
Octadecyl silane chemically bonded to porous silica or ceramic

microparticles, 1.5 to 10 m in diameter, or a monolithic rod.
Gemini-NX C18
Spherical
168
Kinetex XB-C18
Core-shell
180
Kinetex C18
Core-shell
180
Luna C18(2)
Spherical
193
Gemini C18
Spherical
168
Synergi Hydro-RP
Spherical
249
Synergi Fusion-RP
Spherical
249
Onyx C18
Monolith
211
Jupiter C18
Spherical
296
Clarity Oligo-RP
Spherical
301
Clarity Oligo-MS
Core-shell
301
Core-shell
283
Core-shell
283
Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core, 30 to 50 m in diameter.
Porous silica particles, 1.5 to 10 m in diameter, or a monolithic silica rod.
Kinetex Hilic
Core-shell
180
Luna Silica(2)
Spherical
193
Onyx Silica
Monolith
211
Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50 m in diameter.
Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50 m in diameter.
Strong cation-exchange packing: sulfonated fluorocarbon polymer coated on a solid spherical core, 30 to 50 m in diameter.
Core-shell
180
Octyl silane chemically bonded to totally porous silica particles, 1.5 to 10 m in diameter, or a
Kinetex C8
Spherical
193
monolithic silca rod.
Luna C8(2)
Monolith
211
Onyx C8
Core-shell
283
Spherical
193
An essentially monomolecular layer of aminopropyl-silane chemically bonded to totally porous
Luna NH2
silica gel support, 3 to 10 m in diameter.
Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic
Partisil 10 m SCX
Irregular
215, 263
cation-exchange coating, 3 to 10 m in diameter.
Luna 10 m SCX
Spherical
193
Nitrile groups chemically bonded to porous silica particles, 3 to 10 m in diameter.
Luna CN 100
Spherical
193
Capcell CN UG
Spherical
161
Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 m in diameter.
Synergi Polar-RP
Spherical
249
Luna Phenyl-Hexyl
Spherical
193
Gemini C6-Phenyl
Spherical
168
Prodigy PH-3
Spherical
226
Core-shell
180
Strong anion-exchange packing made by chemically bonding a quaternary amine to a solid silica spherical core, 30 to 50 m in diameter.
Trimethylsilane chemically bonded to porous silica particles, 3 to 10 m in diameter.
Develosil TMS-UG (C1) 130
Spherical
164
Silica gel having a chemically bonded, strongly basic quaternary ammonium anion-exchange coating,
PhenoSphere SAX
Spherical
222
5 to 10 m in diameter.
Partisil 10 m SAX
Irregular
215, 263
PartiSphere 5 m SAX
Spherical
263
Hexyl silane chemically bonded to totally porous silica particles, 3 to 10 m in diameter.
PhenoSphere C6
Spherical
222
Dimethyl silane chemically bonded to totally porous silica particles, 5 to 10 m in diameter.
Maxsil RP2 60
Irregular
208
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene
Rezex RHM Monosaccharide
Spherical
228
copolymer in the hydrogen form, 7 to 11 m in diameter.
Rezex ROA
Spherical
228
Amino and cyano groups chemically bonded to porous silica particles, 3 to 10 m in diameter.
Partisil PAC
Irregular
215, 263
Rezex RCM
Spherical
228
copolymer in the calcium form, 9 m in diameter.
Rezex RCU
Spherical
228
Dihydroxypropane groups chemically bonded to porous silica particles, 3 to 10 m in diameter.
Luna HILIC
Spherical
193
Shodex PROTEIN KW-800 series
Spherical
240
TSKgel QC-PAK 200 and 300
Spherical
260
A rigid, spherical styrene-divinylbenzene copolymer, 3 to 10 m in diameter.
PolymerX RP-1
Spherical
224
Phenogel 100
Spherical
216
A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, about 10 m in size.
Rezex ROA
Spherical
228
An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary
Shodex IEC QA-825
Spherical
240
ammonium groups, about 10 m in size.
A semi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups on the matrix surface, 32 to 63 m in diameter.
PolySep-GFC-P2000
Spherical
225
Packing having the capacity to separate compounds with a MW range from 100 to 5000 daltons
(as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers.
Shodex OHpak SB-802.5HQ
Spherical
240
A polymethacrylate resin base, crosslinked with poly-hydroxylated ether (surface contained some residual
carboxyl functional groups) was found suitable.
Butyl silane chemically bonded to totally porous silica particles, 3 to 10 m in diameter.
Jupiter 300 C4
Spherical
296
Aeris WIDEPORE C4
Core-shell
283
Porous silica particles, 30 to 50 m in diameter.
Sepra
Irregular
352
A multifunctional support, which consists of a high purity, 100 , spherical silica substrate that has been bonded with anionic (amine) functionality in addition to a conventional reversed
phase C8 functionality.
Gamma alumina, reversed phase, low carbon percentage by weight, alumina-based polybutadiene spherical particles, 5 m diameter with a pore diameter of 80 .
Ethyl silane chemically bonded to a totally porous silica particle, 3 to 10 m in diameter.
Maxsil RP2 60
Irregular
208
A hydroxide-selective, strong anion-exchange resin-quaternary amine bonded on latex particles attached to a core of 8.5 m macroporous particles having a pore size of 2000 and
consisting of ethylvinylbenzene cross-linked with 55 % divinyl benzene.
Phenomenex
HPLC Column Selection by USP Listing

L32
L38
A chiral ligand-exchange packing- L-proline copper complex covalently bonded to irregularly

shaped silica particles, 5 to 10 m in diameter.
Packing having the capacity to separate dextrans of 4,000 to 500,000 daltons. It is spherical,
silica-based and processed to provide pH stability.
copolymer in the lead form, about 9 m in diameter.
A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular monolayer
bonded phase having a pore size of 150 .
3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5 m aminopropyl silica.
Polymethacrylate gel packing having the capacity to separate proteins by molecular size over a
range of 2,000 to 40,000 daltons.
Methacrylate-based size-exclusion packing for water-soluble samples.
L39
Hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin.
L40
L41
L42
L43
Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 5 m to 20 m in diameter.

Immobilized -acid glycoprotein on spherical silica particles, 5 m in diameter.
Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5 m in diameter.
Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 5 to 10 m in diameter.
L33
L34
L35
L36
L37
L44
L45
L46
L47
L48
L49
L50
L54
L55
L56
L57
L58
L59
L60
L61
L62
L63
L64
L65
L66
L67
L68
L69
L70
L71
L72
L73
L74
L75
Particle Shape
Nucleosil Chiral-1
Spherical
324
BioSep-SEC-S2000
BioSep-SEC-S3000
Rezex RPM Monosaccharide
Spherical
Spherical
Spherical
292
292
228
(BioSep-SEC-S2000
may be used)
Nucleosil Chiral-3
PolySep-GFC-P3000
Shodex OHpak SB-803HQ
PolySep-GFC-P1000
PolySep-GFC-P series
Shodex OHpak SB-802HQ series
Shodex RSpak DM-614
Lux Cellulose-1
Spherical
292
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
324
225
240
225
240
225
240
240
318
Kinetex PFP
Core-shell
Luna PFP(2)
Spherical
A multifunctional support, which consists of a high purity, 60 , spherical silica substrate that has been bonded with a cationic exchanger, sulfonic acid functionality in addition to a
conventional reversed phase C8 functionality.
Beta cyclodextrin bonded to porous silica particles, 5 to 10 m in diameter
Spherical
Nucleodex Beta-PM
Spherical
Polystyrene/divinylbenzene substrate agglomerated with quaternary amine functionalized latex beads, about 9 to 11 m in diameter.
High capacity anion-exchange microporous substrate, fully functionalized with a trimethylamine group, 8 m in diameter.
Sulfonated, cross-linked polystyrene with an outer layer of submicron, porous,anion-exchange microbeads, 15 m in diameter.
A reversed-phase packing made by coating a thin layer of polybutadiene on to spherical porous zirconia particles, 3 to 10 m in diameter.
Multifunction resin with reversed-phase retention and strong anion-exchange functionalities. The resin consists of ethylvinylbenzene, 55 % cross-linked with divinylbenzene copolymer,
3 to 15 m in diameter, and a surface area of not less than 350 m2/g. Substrate is coated with quaternary ammonium functionalized latex particles consisting of styrene cross-linked
with divinylbenzene.
Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 5 to 10 m in diameter.
A strong cation-exchange resin made of porous silica with sulfopropyl groups, 5 to 10 m in diameter.
Weak cation-exchange resin consisting of ethylvinylbenzene, 55 % cross-linked with divinylbenzene copolymer, 3 to 15 m diameter. Substrate is surface grafted with carboxylic acid and/or
phosphoric acid functionalized monomers. Capacity not less than 500 Eq/column.
A size exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads, about 13 m in diameter.
A strong cation-exchange resin made of porous silica coated with polybutadiene-maleic acid copolymer, about 5 m in diameter.
Propyl silane chemically bonded to totally porous silica particles, 3 to 10 m in diameter.
ZORBAX SB-C3
Spherical
A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5 m in diameter,
Ultron ES-OVM
Spherical
with a pore size of 120 angstroms.
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer
Rezex RNM-Carbohydrate
Spherical
in the sodium form, about 6 to 30 m in diameter.
To separate proteins by molecular weight over the range of 5 to 7000 kDa. Spherical 5 to 10 m,
BioSep-SEC-S2000
Spherical
silica-based, and processed to provide hydrophilic characteristics and pH stability.
BioSep-SEC-S3000
Spherical
Spherical, porous silica gel, 10 m or less in diameter, surface has been covalently modified with alkyl amide groups and endcapped.
Hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 13 m microporous particles, pore size less than 10 , and consisting of ethylvinylbenzene
cross-linked with 55 % divinylbenzene with a latex coating composed of 85 nm diameter microbeads bonded with alkanol quaternary ammonium ions (6 %).
C30 silane bonded phase on a fully porous spherical silica, 3 to 15 m in diameter.
Develosil Combi-RP
Spherical
Spherical
Develosil RP-Aqueous-AR
Spherical
Glycopeptide teicoplanin linked through multiple covalent bonds to a 100 spherical silica
Strongly basic anion-exchange resin consisting of 8 % crosslinked styrene divinylibenzene copolymer with a quaternary ammonium group in the chloride form, 45 to 180 m in diameter.
Strongly acidic cation-exchange resin consisting of 8 % sulfonated crosslinked styrene divinylbenzene copolymer with a sulfonic acid group in the hydrogen form, 63 to 250 m
in diameter.
A crown ether coated on a 5 m particle size silica gel substrate. The active site is (S)-18-crown-6-ether.
Porous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group of the polymer,
Asahipak ODP-40
Spherical
2 to 10 m in diameter.
Asahipak ODP-50
Spherical
Spherical, porous silica, 10 m or less in diameter, the surface of which has been covalently modified with alkyl amide groups and not endcapped.
Ethylvinylbenzene/divinylbenzene substrate agglomerated with quaternary amine functionalized 130 nm latex beads, about 6.5 m in diameter.
Cellulose tris (phenyl carbamate) coated on 5 m silica.
A rigid spherical polymethacrylate 4 to 6 m in diameter.
(R)-phenylglycine and 3,5-dinitroaniline urea linkage covalently bonded to silica.
Chirex 3012
Spherical
A rigid spherical polydivinylbenzene particle 5 to 10 m in diameter.
A strong anion-exchange resin consisting of a highly cross-linked core of 7m macroporous particles having a 100 average pore size and linked with 55 % divinylbenzene and an anionexchange layer grafted to the surface, which is functionalized with alkyl quaternary ammonium ions.
A chiral-recognition protein, bovine serum albumin (BSA), chemically bonded to silica particles, about 7m in diameter,
Resolvosil BSA
Spherical
with a pore size of 300
Phenomenex
Page
180
193
313
324
264
325
228
292
292
164
164
164
HPLC | HPLC Column Selection by USP Listing
L51
L52
L53
Column
158
158
314
324
153
HPLC Column Selection by EP Listing

The European Pharmacopeia (Ph. Eur.), or EP, of the Council of
Europe is a pharmacopeia, listing a wide range of active substances
and excipients used to prepare pharmaceutical products in Europe.
It includes general and specific monographs that give quality
standards for all the main medicines used in Europe. All medicines
sold in the 37 Member States of the European Pharmacopeia must
comply with these quality standards so that consumers have a
guarantee for products obtained from pharmacies and other legal
suppliers.
HPLC Column Selection by EP listing | HPLC
Description According to Pharm. Eur. 7.0

4.1.1. Reagents 2010
154
It is widely understood that all HPLC packings are not alike, and no
single column can perform a myriad of desired separations. HPLC
packings differ in hydrophobicity, surface coverage, surface area,
pore size, and particle shape.
For each European Pharmacopeia (Ph. Eur.) or EP description of
the HPLC stationary phase, you will find listed the most suitable
Phenomenex HPLC column. Other possible columns can also be
used for these analyses. Please contact Phenomenex for your
specific LC column needs.
Number
Column
Silica gel -acceptor / -Donor for chiral separations (1-(3,5-dinitrobenzamide)-1,2,3,4tetrahydrophenanthrene).

Silica gel AD for chiral separation.
Silica gel AGP for chiral chromatography. (alpha 1-acid glycoprotein)
Silica gel BC for chiral chromatography. (Beta-Cyclodextrin)
Silica gel for chromatography.
Silica gel for chromatography, alkyl bonded for use with highly aqueous mobile phases.
1160100
Whelk-O 1+2
Silica gel for chromatography, alkyl bonded for use with highly aqueous mobile phases,
endcapped.
1176900
Silica gel for chromatography, amidohexadecylsilyl.

Silica gel for chromatography, aminohexadecylsilyl.
Silica gel for chromatography, aminopropylmethylsilyl.
1170400
1138400
1102400
Silica gel for chromatography, aminopropylsilyl.
1077000
Silica gel for chromatography, Amylose derivative of.

Silica gel for chromatography, butylsilyl. Spheroidal 300 ; pore volume: 0.6 cm3/g; area: 80 m2/g.
Silica gel for chromatography, butylsilyl, endcapped.
1109800
1076200
1170500
Silica gel for chromatography, crown-ether.

Silica gel for chromatography, cyanosilyl.
1178000
1109900
Silica gel for chromatography, di-isobutyloctadecylsilyl.
1140000
Silica gel for chromatography, diisopropylcyanopropylsilyl.

Silica gel for chromatography, dimethyloctadecylsilyl. irregular; area: 300 m2/g.
Silica gel for chromatography, diol dihydroxypropyl, 100 .
Silica gel for chromatography, hexadecylamidylsilyl with hexadecylcarboxamidopropyldimethylsilyl groups.
Silica gel for chromatography, hexadecylamidylsilyl, endcapped with hexadecylcarboxamidopropyldimethylsilyl groups.
Silica gel for chromatography, hexylsilyl.
1168100
1115100
1110000
1162500
Silica gel for chromatography, hexylsilyl, endcapped.
1174400
Silica gel for chromatography, human albumin coated.

Silica gel for chromatography, hydrophilic surface has been modified to provide hydrophilic
characteristics.
Silica gel for chromatography, nitrile cyanopropylsilyl.
1138500
1077200
Silica gel for chromatography, nitrile R1 chemically bonded nitrile groups.
1077400
Silica gel for chromatography, nitrile R2 ultrapure silica (<20 ppm metal) with cyanopropylsilyl
groups.
1119500
Silica gel for chromatography, nitrile, endcapped with cyanopropylsilyl groups.

Silica gel for chromatography, octadecanoylaminopropylsilyl aminopropylsilyl groups which are
acylated with octadecanoyl groups.
1174500
1115200
1171700
1148700
1161300
1076900
1160200
1077300
Page
324
5
Sumichiral OA-7000
Luna Silica(2)
Synergi Hydro-RP
Synergi Fusion-RP
Gemini C18
Gemini-NX C18
Kinetex C18
Kinetex XB-C18
Synergi Hydro-RP
Synergi Fusion-RP
Gemini C18
Gemini-NX C18
Kinetex C18
Kinetex XB-C18
SphereClone NH2 (Amino)

PhenoSphere NH2 (Amino)
SphereClone NH2 (Amino)
PhenoSphere NH2 (Amino)
Lux Amylose-2
Jupiter 300 C4
Aeris WIDEPORE C4
Sumichiral OA-8000
Luna CN (Cyano)
HyperClone CN (Cyano)
PhenoSphere CN (Cyano)
ZORBAX StableBond C18
Kinetex XB-C18
ZORBAX StableBond CN
Bondclone C18
Luna HILIC
1172400
1077100
Indicated Particle Size (m)
3 to 10
3 to 10
3 to 10
3 to 10
10
3 to 10
3 to 10
3 to 10
10
5
5
326
193
249
249
168
168
180
180
249
249
168
168
180
180
247
222
247
222
318
296
283
326
193
177
222
264
180
264
160
193
5
SphereClone C6
PhenoSphere C6
SphereClone C6
PhenoSphere C6
Luna HILIC
Luna CN (Cyano)
Luna CN (Cyano)
Luna CN (Cyano)
Luna CN (Cyano)
3 to 10
3 to 10
3 to 10
3 to 10
3 to 10
3 to 10
3 to 10
3 to 10
247
222
247
222
193
193
177
222
193
177
222
193
177
222
193
Phenomenex

Number
Silica gel for chromatography, octadecylsilyl.
1077500
Silica gel for chromatography, octadecylsilyl R1 ultrapure silica (<20 ppm metals), pore size and
C-load are indicated in the method.
1110100
Silica gel for chromatography, octadecylsilyl R2 ultrapure silica; 150 pore size; 20 % C-load;
optimized for the analysis of PAHs.
Silica gel for chromatography, octadecylsilyl, base-deactivated pretreated before the bonding by
careful washing and hydrolyzing most of the superficial siloxane bridges to minimize the interaction with basic components.
1115300
Silica gel for chromatography, octadecylsilyl, endcapped. To minimize any interaction with basic
compounds its carefully endcapped to cover most of the remaining silanol groups.
1115400
Silica gel for chromatography, octadecylsilyl, endcapped R1ultrapure silica (<20 ppm metal),
19 % C-load. To minimize any interaction with basic compounds its carefully endcapped to cover
most of the remaining silanol groups.
Silica gel for chromatography, octadecylsilyl, endcapped, base-deactivated; pore size 100 ,
C-load:16 %, pretreated before the bonding by careful washing and hydrolysing most of the superficial siloxane bridges. To further minimize any interaction with basic compounds its carefully
endcapped to cover most of the remaining silanol groups.
Silica gel for chromatography, octadecylsilyl, endcapped, base-deactivated R1; pretreated before
the bonding by careful washing and hydrolyzing most of the superficial siloxane bridges. To
further minimize any interaction with basic compounds its carefully endcapped to cover most of
the remaining silanol groups.
1115401
Silica gel for chromatography, octadecylsilyl, monolithic.

Silica gel for chromatography, octadecylsilyl, with polar embedded groups, endcapped; the
particles are based on a mixture of silica chemically modified at the surface by the bonding of
octadecylsilyl groups and silica chemically modified with a reagent providing a surface with
chains having embedded polar groups.
Silica gel for chromatography, octadecylsilyl, with polar incorporated groups, endcapped; the
particles are based on silica, chemically modified with a reagent providing a surface with chains
having polar incorporated groups and terminating octadecyl groups.
Silica gel for chromatography, octylsilyl.
Silica gel for chromatography, octylsilyl R1.

Bonding of octylsilyl and methyl groups (double bonded phase).
1077701
Silica gel for chromatography, octylsilyl R2 ultrapure silica (<20 ppm metal); pore size 100 ;
C-load: 19 %.
Silica gel for chromatography, octylsilyl R3 ultrapure silica, bonding of octasilyl groups and sterically protectedwith branched hydrocarbons at the silanes.
Silica gel for chromatography, octylsilyl, base-deactivated pretreated before the bonding by careful washing and hydrolyzing most of the superficial siloxane bridges to minimize the interaction
with basic components.
1077702
Silica gel for chromatography, octylsilyl, endcapped. To minimize any interaction with basic
1119600
Silica gel for chromatography, octylsilyl, endcapped, base-deactivated pretreated before the
bonding by careful washing and hydrolyzing most of the superficial siloxane bridges to minimize
the interaction with basic components. To further minimize any interaction with basic compounds
its carefully endcapped to cover most of the remaining silanols.
Silica gel for chromatography, octylsilyl, with polar incorporated groups, endcapped; chains having polar incorporated groups and terminatingoctyl groups.
Silica gel for chromatography, palmitamidopropylsilyl, endcapped bonding with palmitamidopropyl groups and endcapped with acetamidopropyl groups.
1148800
Phenomenex
Column
Page
Luna C18(2)
Synergi Hydro-RP
Synergi Fusion-RP
Gemini C18
Gemini-NX C18
HyperClone C18
Kinetex C18
Kinetex XB-C18
SphereClone C18 ODS(1) or (2)
Luna C18(2)
Synergi Hydro-RP
Synergi Fusion-RP
Gemini C18
Gemini-NX C18
Jupiter C18
Kinetex C18
Kinetex XB-C18
EnviroSep-PP
Prodigy ODS-2
Luna C18(2)
Gemini C18
Gemini-NX C18
Kinetex C18
Kinetex XB-C18
Luna C18(2)
Gemini C18
Gemini-NX C18
Kinetex C18
Kinetex XB-C18
Luna C18
Synergi Hydro-RP
3 to 10
3 to 10
1154500
1177900
Luna C18(2)
Prodigy ODS-3
Gemini C18
Gemini-NX C18
Luna C18(2)
Gemini C18
Gemini-NX C18
Kinetex C18
Kinetex XB-C18
Onyx C18
(Synergi Fusion-RP)
3 to 10
193
226
168
168
193
168
168
180
180
211
249
1165100
Synergi Fusion-RP
3 to 10
249
1077700
Luna C8(2)
Prodigy C8
HyperClone C8 (MOS)
Kinetex C8
SphereClone C8
Luna C8(2)
Prodigy C8
HyperClone C8 (MOS)
Kinetex C8
SphereClone C8
3 to 10
193
226
177
180
246
193
226
177
180
246
1077600
1108600
1162600
1155200
1131600
Luna C8(2)
Prodigy C8
HyperClone C8 (BDS)
Kinetex C8
Luna C8(2)
Prodigy C8
HyperClone C8 (BDS)
Kinetex C8
Luna C8(2)
Prodigy C8
Kinetex C8
HyperClone C8 (BDS)
3 to 10
3 to 10
3 to 10
3 to 10
3 to 10
193
249
249
168
168
177
180
180
246
193
249
249
168
168
296
180
180
166
226
193
168
168
180
180
193
168
168
180
180
193
249
HPLC | HPLC Column Selection by EP Listing

264
3 to 10
3 to 10
3 to 10
1152600
3 to 10
1161900
3 to 10
193
226
177
180
193
226
177
180
193
226
180
177
155
HPLC Column Selection by EP listing | HPLC
156

Number
Silica gel for chromatography, phenylhexylsilyl.
1153900
Silica gel for chromatography, phenylhexylsilyl, endcapped. 3 m; To minimize any interaction

with basic compounds its carefully endcapped to cover most of the remaining silanol groups.
1170600
Silica gel for chromatography, phenylsilyl.
1110200
Silica gel for chromatography, phenylsilyl, R1 spherical silica; pore size: 80 ; surface area:
180m2/g; C-load: 5.5 %.
Silica gel for chromatography, phenylsilyl, endcapped. To minimize any interaction with basic
1075700
Silica gel for chromatography, propoxybenzene, endcapped

Silica gel for chromatography, propylsilyl.
Silica gel for chromatography, strong anion-exchange bonding of quaternary ammonium groups;
pH limit of use: 2 to 8.
Silica gel for chromatography, strong cation-exchange bonding of sulfonic acid groups.
Silica gel for chromatography, trimethylsilyl.
1174600
1170700
1077800
Silica for size-exclusion chromatography. 10 m silica with a very hydrophilic surface. Pore size
average: 30 nm; pH stability 2 to 8; exclusion range for proteins: 1 x 103 to 3 x 105.
Silica gel OC for chiral separations.
Silica gel OD for chiral separations.
Organosilica polymer, amorphous, octadecylsilyl. Synthetic, spherical hybrid particles containing
both inorganic (silica) and organic (organosiloxanes) components, chemically modified at the
surface by trifunctionally bonded octadecylsilyl groups.
Organosilica polymer, amorphous, octadecylsilyl, endcapped. Synthetic, spherical hybrid particles
containing both inorganic (silica) and organic (organosiloxanes) components, chemically modified
at the surface by trifunctionally bonded octadecylsilyl groups. To minimize any interaction with
basic compounds, it is carefully endcapped to cover most of the remaining silanol groups.
Organosilica polymer, amorphous, polar embedded, octadecylsilyl, endcapped. Synthetic, spherical hybrid particles containing both inorganic (silica)and organic (organosiloxanes) components,
chemically modified at the surface by the bonding of polar embedded octadecylsilyl groups. To
minimize any interaction with basic compounds, it is carefully endcapped to cover most of the
remaining silanol groups.
Organosilica polymer, amorphous, polar embedded propyl-2-phenylsilyl, endcapped.Synthetic,
spherical hybrid particles containing both inorganic (silica)and organic (organosiloxanes) components, chemically modified at the surface by the bonding of polar embedded propyl-2-phenylsilyl
groups. To minimize any interaction with basic compounds, it is carefully endcapped to cover
most of the remaining silanol groups.
Organosilica polymer for mass spectrometry, amorphous, octadecylsilyl, endcapped. Synthetic,
spherical hybrid particles containing both inorganic (silica)and organic (organosiloxanes) components. To minimize any interactionwith basic compounds, it is carefully endcapped to cover most
of theremaining silanol groups.
Vinyl Polymer for Chromatography, octadecyl. Spherical particles (5 m) of a vinyl alcohol copolymer, bonding of octadecyl groups on the hydroxyl groups.
Vinyl Polymer for Chromatography, octadecylsilyl. Spherical particles (5 m) of a vinyl alcohol
copolymer bonded to an octadecylsilane. C-load: 17 %.
Ion-exclusion resin for chromatography. A resin with sulfonic acid groups attached to a polymer
lattice consisting of polystyrene cross-linked with divinylbenzene.
Cation-exchange resin, strong. Strong cation exchange resin in protonated form with sulfonic acid
groups attached to a polymer lattice consisting of polysterene cross-linked with divinylbenzene.
Cation-exchange resin (Calcium form), strong. Resin in calcium form with sulfonic acid groups
attached to a polymer lattice consisting of polystyrene cross-linked with 8 % divinylbenzene
Cation-exchange resin (Sodium form), strong. Resin in sodium form with sulfonic acid groups
attached to a polymerlattice consisting of polystyrene cross-linked with divinylbenzene.
1077900
1154900
1161400
1115500
1146800
1110300
1144200
1178600
Column
Luna Phenyl-Hexyl
Gemini C6-Phenyl
Luna Phenyl-Hexyl
Gemini C6-Phenyl
Synergi Polar-RP
Luna Phenyl-Hexyl
Gemini C6-Phenyl
Prodigy Phenyl-3 (PH3)
SphereClone Phenyl
ZORBAX StableBond Phenyl
(SphereClone Phenyl)
Synergi Polar-RP
Luna Phenyl-Hexyl
Gemini C6-Phenyl
Prodigy Phenyl-3 (PH3)
Synergi Polar-RP
PhenoSphere SAX
3 to 10
Luna SCX
Develosil TMS-UG (C1)
Capcell Pak C1 UG
PhenoSphere C1
BioSep-SEC-S3000
5 to 10
3 to 10
Lux Cellulose-1
Gemini C18
Gemini-NX C18
3
5 to 10
5
5 to 10
3 to 10
3 to 10
3 to 10
10
5
5
Page
180
193
168
193
168
180
249
193
168
226
246
180
264
246
249
193
168
226
180
249
264
222
193
164
161
222
292
318
168
168
Gemini C18
Gemini-NX C18
168
168
1164900
Gemini C18
Gemini-NX C18
168
168
1155400
Asahipak ODP-50
158
1121600
Asahipak ODP-50
158
1131000
Rezex ROA-Organic Acid

Rezex RHM-Monosaccharide
Rezex RHM-Monosaccharide
Rezex RCM-Monosaccharide
Rezex RCU-USP Sugar Alcohols
1150600
1178100
1156800
1104600
1176100
228
228
228
228
228
228
228
228
Phenomenex
Aqua
A C18 Column with Polar

Endcapping
Use Synergi Hydro-RP, an Improved

Alternative to Aqua 125
See p. 249
Packing Material
AQUA C18
AQUA C18
Particle
Shape/Size
(m)
Pore
Size
()
Pore
Volume
(mL/g)
Surface
Area
(m2/g)
Carbon
Load
%
Calculated Bonded
Phase Coverage
(mole/m2)
End
Capping
Spher. 3, 5, 10, 15
Spher. 5, 10, 15
125
200
1.05
1.15
320
215
15
11
N/A
N/A
Proprietary
Proprietary
125 Aqua C18 Column

Aquas polar endcapping produces a surface chemistry that is well
suited for the analysis of small peptides. This chemistry...
makes it an excellent column for smaller, basic peptides
allows for faster column equilibration in gradient analyses
ensures a surface that can be wetted with aqueous
trifluoroacetic acid (TFA)
200 Aqua C18 Column
While 125 Aqua C18 columns are excellent for small pharmaceutical and peptide analyses, some large pharmaceutical and small-to
medium-sized proteins [up to 55,000 MW] are best separated and
purified on 200 Aqua C18 columns. Very efficient analyses are
obtained because of the rapid mass transfer of the proteins into and
out of the pores of the hydrophilic stationary phase. Recoveries are
typically greater than 90 %.
Increased pore size for enhanced diffusion of

large pharmaceuticals and biomolecules
Reduced surface area for faster analyses
and greater sample throughput
HPLC |
3 m Minibore, Analytical, LC/MS and CombiChem Columns (mm)

Phases
30 x 2.0
75 x 2.0
150 x 2.0
C18 125
00A-4311-B0
00C-4311-B0
00F-4311-B0
5 m Minibore, MidBore and LC/MS Columns (mm)

Phases
50 x 2.0
150 x 2.0
C18 125
C18 200
00B-4299-B0
00F-4299-B0
00F-4331-B0
75 x 4.6
100 x 4.6
150 x 4.6
00C-4311-E0
00D-4311-E0
00F-4311-E0
250 x 2.0
150 x 3.0
250 x 3.0
00G-4299-B0
00F-4299-Y0
00G-4299-Y0
SecurityGuard Cartridges (mm)

4 x 2.0*
4 x 3.0*
/10pk
/10pk
AJ0-7510
AJ0-7511
for ID: 2.03.0 mm
3.28.0 mm

4 x 2.0*
4 x 3.0*
/10pk
/10pk
AJ0-7510
AJ0-7511
AJ0-7510
AJ0-7511
for ID: 2.03.0 mm
5 m Analytical, CombiChem, SemiPrep and Preparative Columns (mm)

Phases
50 x 4.6
75 x 4.6
150 x 4.6
250 x 4.6
C18 125
C18 200
00B-4299-E0
00C-4299-E0
00F-4299-E0
00F-4331-E0
00G-4299-E0
00G-4331-E0
250 x 10
150 x 21.2
250 x 21.2
00G-4299-N0
00F-4299-P0
00G-4299-P0
for ID:
For SecurityGuard Cartridge Holders and Cartridges, see p. 235
Phenomenex
Aqua
3.28.0 mm

4 x 3.0*
10 x 10
15 x 21.2**
/10pk
/3pk
/ea
AJ0-7511
AJ0-7512
AJ0-7843
AJ0-7511
AJ0-7512
AJ0-7843
3.2 8.0 mm
9 16 mm
1829 mm
*SecurityGuard Analytical Cartridges require holder, Part No.: KJ0-4282

SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ0-7220
**PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8223
157
Asahipak
By Showa Denko K.K.

High efficiency polymer-based columns
Wide range of pH stability
Wide variety of separation modes
Shodex manufactures a wide variety of HPLC supports under the
Asahipak name that are all based upon a backbone of vinyl alcohol
copolymers. From this hard gel polymer base, Asahipak columns
are manufactured for reversed phase, normal phase, ion-exchange,

aqueous (GFC) and non-aqueous (GPC) size-exclusion, and multimode applications.
These gels provide reproducible results for a wide variety of applications, especially for pharmaceuticals and biomolecules. Additionally, they are able to perform some separations not usually
possible on other HPLC columns.
Separation Modes and Column Selection Process

Separation
Mode
pH
Range
Molecular
Weight Limit
Appropriate
Column to use:
ODP-40
Reversed Phase
ODP-50
2~12
C8P-50
Hydrophilic
Substances
C4P-50
Normal and
Reversed Phase
(amino column)
2~11
NH2P-50
<3,000
GS-220 HQ
<40,000
GF-310 HQ
2~12
<40,000
GS-320 HQ
2~9
<300,000
GF-510 HQ
2~12
<300,000
GS-520 HQ
<2,000,000
GS-620 HQ
HPLC
2~9
GFC
Asahipak
2~9
GF-710 HQ
<10,000,000
GF-7M HQ
Asahipak
ES-502C
Ion-Exchange
2~12
ES-502N
<3,000
GS-220 HQ
<2,000,000
GS-620 HQ
<40,000
GS-320 HQ
<300,000
GS-520 HQ
2~9
Multi-mode
2~12
ODP-40
Reversed Phase
ODP-50
2~12
C8P-50
Hydrophobic
Substances
C4P-50
GPC
158
2~9
<40,000
GF-310 HQ
<300,000
GF-510 HQ
<10,000,000
GF-710 HQ
<10,000,000
GF-7M HQ
Phenomenex
Asahipak
By Showa Denko K.K.

SEC Columns (GF and GS-HQ Series)
Semi-Standard Type (300 x 7.6 mm)
Particle Size
Eluents
Mfr. No.
Part No.
(m)
Multi-Solvent
GF-310 HQ
CH0-2905
5
Multi-Solvent
GF-510 HQ
CH0-2906
5
Multi-Solvent
GF-710 HQ
CH0-2907
9
Multi-Solvent
GF-7M HQ
CH0-2908
7
Multi-Mode
GS-220 HQ
CH0-2909
6
Multi-Mode
GS-320 HQ
CH0-2910
6
Multi-Mode
GS-520 HQ
CH0-2911
7
Multi-Mode
GS-620 HQ
CH0-2912
7
Exclusive M.W.
(Pullulan)
40,000
300,000
10,000,000
10,000,000
3,000
40,000
300,000
2,000,000
NTP*
(per 500 mm)
>16,000
>16,000
>10,000
>11,000
>16,000
>16,000
>15,000
>15,000
Flow Rate
Normal
0.4 - 0.6
0.4 - 0.6
0.4 - 0.6
0.4 - 0.6
0.4 - 0.6
0.4 - 0.6
0.4 - 0.6
0.4 - 0.6
Flow Rate
Max.
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
pH Range
2-9
2-9
2-9
2-9
2-9
2 - 12
2 - 12
2-9
Price
*Number of Theoretical Plates
Guard Columns (50 x 7.6 mm)

Eluents
Mfr. No.
Multi-Solvent
GF-1G 7B
Multi-Mode
GS-2G 7B
Part No.
CH0-2917
CH0-3909
Ion-Exchange Chromatography Columns

Analytical (100 x 7.6 mm)
Particle Size Ion Exchange
Mfr. No.
Part No.
(m)
Group
ES-502C 7C
CH0-2609
9
-COOH
ES-502N 7C
CH0-2610
9
-N(C2H5)2
For full line of Calibration Standards,

see p. 367
Price
Exchange
NTP*
Capacity (meq/g) (per 100 mm)
0.55
>3,000
0.55
>3,000
Flow Rate
Normal
1.0
1.0
Flow Rate
Max.
1.5
1.5
pH Range
2 - 12
2 - 12
Price
*Number of Theoretical Plates.
Part No.
CH0-5582
CH0-5583
CH0-2628
CH0-3374
CH0-2904
CH0-8962
CH0-8963
Column Size
(mm)
150 x 2.0
10 x 2.0
250 x 4.6
150 x 4.6
10 x 4.6
250 x 3.0
10 x 3.0
Particle Size
(m)
5
5
5
5
5
4
5
Flow Rate
Normal
0.05 - 0.2
0.05 - 0.2
0.5 - 1.0
0.5 - 1.0
0.5 - 1.0
Flow Rate
Max.
0.4
0.4
1.5
1.5
1.5
pH Range
2 - 11
2 - 11
2 - 11
2 - 11
2 - 11
Price
Asahipak
Mfr. No.
NH2P-50 2D
NH2P-50G 2A**
NH2P-50 4E
NH2P-50 4D
NH2P-50G 4A**
NH2P-40 3E
NH2P-50G 3A**
HPLC |
Amino Columns
Analytical
**Guard column
Polymer-Based for Hydrophilic Interaction Chromatography (HILIC)
Reversed-Phase Columns
Analytical
Mfr. No.
0DP-40 4D
0DP-40 4E
ODP-50 2D
ODP-50 4B
ODP-50 4D
ODP-50 4E
ODP-50 6D
C8P-50 4D
C4P-50 4D
C4P-50 4E
Part No.
CH0-7163
CH0-7164
CH0-5584
CH0-5585
CH0-2613
CH0-2614
CH0-2615
CH0-2617
CH0-2619
CH0-2620
Column Size
(mm)
150 x 4.6
250 x 4.6
150 x 2.0
50 x 4.6
150 x 4.6
250 x 4.6
150 x 6.0
150 x 4.6
150 x 4.6
250 x 4.6
Particle Size
(m)
Alkyl Group
4
C18
4
C18
5
C18
5
C18
5
C18
5
C18
5
C18
5
C8
5
C4
5
C4
C%
17
17
17
17
17
17
17
10
6
6
Flow Rate
Normal
0.3 - 0.6
0.3 - 0.6
0.05 - 0.2
0.5 - 0.8
0.5 - 0.8
0.5 - 0.8
0.5 - 1.0
0.5 - 0.8
0.5 - 0.8
0.5 - 0.8
Flow Rate
Max.
pH Range
0.8
2 - 12
0.8
2 - 12
0.4
2 - 12
1.5
2 - 12
1.5
2 - 12
1.5
2 - 12
2.5
2 - 12
1.0
2 - 12
1.0
2 - 12
1.0
2 - 12
Flow Rate
Normal
0.5 - 0.8
0.5 - 1.0
Flow Rate
Max.
1.5
2.5
Price
Guard Columns
Mfr. No.
ODP-50G 4A
ODP-50G 6A
Part No.
CH0-2914
CH0-2913
Column Size
(mm)
10 x 4.6
10 x 6.0
Particle Size
(m)
Alkyl Group
5
C18
5
C18
C%
17
17
pH Range
2 - 12
2 - 12
Price
Other column dimensions and preparative columns available upon request.
Phenomenex
159
Bondclone
If Bondclone analytical columns do not

provide you with at least equivalent
separations to a Bondapak column
of the same phase, particle size and
dimension, return the column with
comparative data within 45 days for a
FULL REFUND.
Guaranteed Replacement to Bondapak

Highly reproducible
Long column life
Mimics performance of Waters Bondapak
Economically priced
Phenomenex Bondclone columns have been developed to provide
chromatographic behavior that mimics that of Waters Bondapak
columns. For comparative applications, please contact your local
Phenomenex representative.
Bondclone Reproducibility
Phenomenex Bondclone material is made in huge batches (several
years supply at a time), so column-to-column reproducibility is
excellent. Bondclone columns are always in stock and available for
immediate shipment.
Table 1. Bondclone Silica Physical Properties

Nominal
Particle Size
10 m
BET
Surface Area
296.0 m2/g
Pore
Volume*
1.1 cc/g
Pore
Size**
148.7
Bondclone
HPLC
*Single point total pore volume.

**Average pore diameter (4V/A by BET).
Data provided by an independent laboratory.
Waters
Description (mm)
Bondapak C18
300 x 3.9
Bondapak C18
150 x 3.9
and Cross-Reference Chart

Phenomenex
Part No.
Part No.
WAT027324
00H-2117-C0
Bondapak C18
Radial-Pak Cartridge
100 x 8
Bondapak Phenyl
300 x 3.9
Bondapak CN
150 x 3.9
Bondapak NH2
300 x 3.9
Porasil Silica
300 x 3.9
WAT086684
00F-2117-C0
00G-2117-E0
WAT085721
00D-2117-L0
WAT027198
00H-3129-C0
WAT086688
00F-3127-C0
00H-3127-C0
WAT084040
00H-3128-C0
WAT027477
00H-2119-C0
Description (mm)
Bondclone C18
300 x 3.9
Bondclone C18
150 x 3.9
Bondclone C18
250 x 4.6
Bondclone C18
100 x 8
(S.S. Column)
Bondclone Phenyl
300 x 3.9
Bondclone CN
150 x 3.9
Bondclone CN
300 x 3.9
Bondclone NH2
300 x 3.9
Bondclone Silica
300 x 3.9
Bondclone phenyl phase uses a different silica than other phases in the Bondclone series.
Price
SecurityGuard
Cartridges (mm)
4 x 3.0
/ 10pk
AJ0-4287
AJ0-4287
AJ0-4287
AJ0-4287
AJ0-4351
AJ0-4305
AJ0-4305
AJ0-4302
AJ0-4348
for ID: 3.2-8.0mm
SecurityGuard Analytical Cartridges require
universal holder Part No.: KJO-4282
Guard Column
Size (mm)
Conventional Guard Column
30 x 3.9
C18
Price
03A-2117-C0
Bondclone is a trademark of Phenomenex, Inc.

Bondapak is a registered trademark of Waters Corporation. Porasil is a trademark of Waters Corporation.
160
Phenomenex
Capcell Pak
By Shiseido Co., Ltd.

Extended pH range from 1-10 provides longer lifetimes with harsh mobile phases
Novel bonding reduces residual silanols which cause peak tailing
Polymer coated capsule type silica
Excellent selectivity for polar compounds
Capcell Pak columns, manufactured by Shiseido Co., Ltd., Japan, distributed worldwide by
Phenomenex, are durable, reproducible columns designed to eliminate secondary retention effects that lead to tailing peaks. A two-step synthesis is used: 1) surface coating of
the base silica gel using a reinforced silicone polymer by vapor deposition, resulting in a
homogenous monolayer, and 2) bonding of alkyl groups to the coated surface to shield any
acidic silanols. This process provides excellent peak shape for acidic, basic and chelating
compounds. Eight types of Capcell Pak columns are available in sizes ranging from analytical to preparative.
Table 1. Performance Overview of Capcell Pak Media

Type
Base Silica Gel
Polymer Coating
pH Range
Separation Quality
Retention of Polar Compounds
AG
SG
UG
MG
Conventional grade
High purity (metal content: <5 ppm)
2-10
2-9
2-10
2-10
Good
Good
Excellent
Excellent (LC-MS)
Fair
Fair
Fair
Strong
MG-II
2-10
Excellent (LC-MS)
Excellent
ACR
1-10
Excellent (LC-MS)
Fair
DD
1.5-10
Excellent (LC-MS)
Excellent
AQ
Mono-layer
Mono-layer
Homogeneous mono-layer
Controlled homogeneous
mono-layer
Reinforced homogeneous
mono-layer
mono-layer
mono-layer
mono-layer
2-9
Excellent (LC-MS)
Excellent
Choose from Several Different Capcell Pak C18 Phases:
HPLC
C18 MG and C18 MG-II

C18 MG employs a silica gel with a relatively large surface area to which a controlled amount
of C18 phase is introduced. This makes it a well-balanced stationary phase in terms of its
retention behavior towards polar and hydrophobic compounds. Especially noteworthy is its
ability to separate polar compounds. The new C18 MG-II offers an even higher level of silanol
shielding, making it excellent for analysis of basic compounds under neutral conditions - a
special advantage to LC/MS users.
| Capcell Pak
C18 UG120
The moderate hydrophobicity of C18 UG120 is recommended for those who wish to perform
their analysis in a relatively short time frame. The nonpolar surface of C18 UG120 is especially
suitable for eluting polar compounds rapidly and sharply.
C18 ACR
C18 ACR shows excellent durability to acidic mobile phases. It is recommended to those who
are not satisfied with the performance of their columns under harsh acidic conditions. The
phase shows similar retention properties to those of UG, but in addition, shows high steric
selectivity that is analogous to polymeric phases.
C18 AQ
The C18 AQ phase was developed to retain highly polar compounds, and is useable in 100 %
aqueous-based mobile phases.
Column Selection By Application

Parameters
General-purpose
Harsh Acidic Conditions
Preparative Applications
Fast Analysis
Acid Resistance
Alkaline Resistance
Column Pressure
Polar Compounds
Phenomenex
Recommended C18 Phase

MG-II, MG
ACR, DD
UG80
MG-II, MG S3, UG120
ACR > DD > MG-II, MG > AG > UG
MG-II, MG > ACR > DD > UG > AG
DD < UG < ACR < AQ < MG-II, MG
AQ > DD > MG-II, MG
161
Capcell Pak
Capcell Pak
| HPLC

UG Series
Part No.
Mfr. No.
C8 UG 120 5 m
CH0-3827
70001
CH0-3831
71503
CH0-3832
71504
Phenyl UG 120 5 m
CH0-3840
73503
CH0-3841
73504
CN UG 120 5 m
CH0-3857
64501
CH0-3858
64503
CH0-3859
64504
C18 UG 120 3 m
CH0-4831
69790
CH0-4832
69791
CH0-4833
69792
CH0-3878
61526
CH0-3880
61530
CH0-3881
61533
C18 UG 120 5 m
CH0-3812
70501
CH0-3813
70503
CH0-3814
70504
CH0-3815
61501
CH0-3816
61503
CH0-3817
61504
CH0-3819
61514
CH0-3820
61521
CH0-3821
61524
CH0-3822
61532
CH0-3823
61534
C18 UG 120 Guard Cartridge
CH0-3895
12412
C1 UG 120 5 m
CH0-3850
63504
NH2 UG 80 5 m
CH0-3864
75003
CH0-3867
62503
CH0-3868
62504
SCX UG 80 5 m
CH0-3882
77003
CH0-3883
77004
C18 UG 300 5 m
CH0-3875
70801
Dimensions (mm)
Unit
Price
35 x 2.0
150 x 4.6
250 x 4.6
ea
ea
ea
150 x 4.6
250 x 4.6
ea
ea
35 x 4.6
150 x 4.6
250 x 4.6
ea
ea
ea
50 x 2.0
75 x 2.0
100 x 2.0
50 x 4.6
100 x 4.6
150 x 4.6
ea
ea
ea
ea
ea
ea
35 x 2.0
150 x 2.0
250 x 2.0
35 x 4.6
150 x 4.6
250 x 4.6
250 x 10
35 x 20
250 x 20
50 x 30
250 x 30
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
10 x 4.0
2/pk
250 x 4.6
ea
150 x 2.0
150 x 4.6
250 x 4.6
ea
ea
ea
150 x 4.6
250 x 4.6
ea
ea
35 x 2.0
ea
SG Series
Part No.
Mfr. No.
C18 SG 120 5 m
CH0-3941
12512
CH0-3942
12513
C18 SG 120 Guard Cartridge
CH0-3893
12411
162
Dimensions (mm)
Unit
35 x 1.0
35 x 2.0
50 x 2.0
75 x 2.0
50 x 4.6
75 x 4.6
35 x 20
50 x 20
ea
ea
ea
ea
ea
ea
ea
ea
35 x 2.0
150 x 2.0
150 x 3.0
250 x 3.0
150 x 4.6
250 x 4.6
250 x 10
50 x 20
250 x 20
ea
ea
ea
ea
ea
ea
ea
ea
ea
10 x 4.0
2/pk
MG-II Series
Part No.
Mfr. No.
Dimensions (mm)
Unit
C18 MG-II 100 5 m - Silanol Shielding (pH 2-10)
CH0-7622
92528
35 x 4.6
ea
CH0-7623
92532
150 x 4.6
ea
CH0-7624
92533
250 x 4.6
ea
CH0-7625
92516
35 x 2.0
ea
CH0-7626
92520
150 x 2.0
ea
CH0-7628
92503
35 x 1.0
ea
C18 MG-II 100 5 m - Silanol Shielding (pH 2-10) Guard Cartridges
CH0-7631
12200
10 x 4.0
2/pk
CH0-7632
12199
10 x 2.0
2/pk
ACR Series
Part No.
Mfr. No
Dimensions (mm)
C18 ACR 80 5 m Acid Resistance (pH 1-10)
CH0-7303
91103
150 x 4.6
CH0-7304
91104
250 x 4.6
Preparative sizes also available for all AG, SG and UG phases.
AG Series
Part No.
Mfr. No.
C18 AG 120 5 m
CH0-3976
12503
CH0-3977
12504
C18 AG 120 Guard Cartridge
CH0-3894
12410
MG Series
Part No.
Mfr. No.
C18 MG 100 3 m
CH0-7137
90841
CH0-7140
90821
CH0-7141
90822
CH0-7142
90826
CH0-7147
90802
CH0-7148
90806
CH0-7151
90862
CH0-7152
90863
C18 MG 100 5 m
CH0-5839
90301
CH0-5840
90303
CH0-5837
90203
CH0-5838
90204
CH0-5834
90103
CH0-5835
90104
CH0-7154
90604
CH0-7155
90702
CH0-7156
90704
C18 MG 100 5 m Guard Cartridges
CH0-7158
12496
Dimensions (mm)
Unit
150 x 4.6
250 x 4.6
ea
ea
10 x 4.0
2/pk
Dimensions (mm)
Unit
150 x 4.6
250 x 4.6
ea
ea
10 x 4.0
2/pk
Price
Price
AQ Series
Part No.
Mfr. No
Dimensions (mm)
C18 AQ 5 m Aqueous, High Polarity (pH 2-9)
CH0-7381
92044
150 x 4.6
CH0-7382
92045
250 x 4.6
CH0-7383
92010
35 x 2.0
CH0-7384
92014
150 x 2.0
C18 AQ 5 m Guard Cartridges
CH0-7391
12170
10 x 2.0
CH0-7392
12180
10 x 4.0
Cartridge Holders
Part No.
Mfr. No.
CH0-3902
EF4010
CH0-3903
EF4020
Description
Holder for 10 mm cartridges
Holder for 20 mm cartridges
Unit
Price
Price
Price
ea
ea
Unit
Price
ea
ea
ea
ea
2/pk
2/pk
Unit
ea
ea
Price
Phenomenex
Chromolith
For Availability and Ordering Information please contact your Phenomenex Technical Consultant.
For Alternative Monolithic Silica Rod Format for Reversed Phase Separations, see Onyx p. 211.
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
Columbus
For new methods, we recommend Gemini-NX HPLC columns, see p. 168
Cosmosil
By Nacalai Tesque
C18-AR-II and PBB phases available
Unusual PYE and NPE phases for isomer separations
Preparative scale columns available
Price
Cosmosil Columns (continued)

Part No.
Mfr. No.
5 m NPE
CH0-3105
379-02
CH0-4169
379-90
Buckyprep
CH0-4170
379-83
CH0-3113
379-77
Description
Size (mm)
5 m NPE
5 m NPE
150 x 4.6
250 x 4.6
Buckyprep Guard Column

Buckyprep
10 x 4.6
250 x 4.6
Chromolith / Columbus / COSMOSIL / CUROSIL
All other Nacalai Tesque/Cosmosil columns are available

on request.
Price
HPLC |
Cosmosil Columns
Part No.
Mfr. No.
Description
Size (mm)
5 m C18 - AR [Only use if specified by your method. Use C18-AR-II for all
new applications]
CH0-3059
378-58
5 m C18-AR Guard Column
10 x 4.6
CH0-3063
378-61
5 m C18-AR
150 x 4.6
CH0-3064
378-62
5 m C18-AR
250 x 4.6
5 m C18 - AR-II
CH0-4158
381-41
5 m C18-AR-II Guard
10 x 4.6
Column
CH0-4163
381-44
5 m C18-AR-II
150 x 4.6
CH0-4164
381-45
5 m C18-AR-II
250 x 4.6
5 m C18 - MS
CH0-3071
379-71
5 m C18-MS
150 x 4.6
CH0-3072
379-72
5 m C18-MS
250 x 4.6
5 m C18 - AR - 300
CH0-3085
379-14
5 m C18-AR-300
250 x 4.6
5 m PYE
CH0-3092
379-03
5 m PYE Guard Column
10 x 4.6
CH0-3467
750-01
5 m PYE
50 x 4.6
CH0-3095
378-37
5 m PYE
150 x 4.6
CH0-3469
379-69
5 m PYE
250 x 4.6
Curosil
For new methods, we recommend Luna PFP columns, see p. 193
Phenomenex
163
Develosil
By Nomura Chemical Co.

RP-Aqueous and RP-Aqueous-AR (C30) work with 100 %
aqueous mobile phases and give added polar compound
retention
Supplied worldwide (except Japan) by Phenomenex, Develosil

HPLC columns provide high efficiency, high resolution separations.
Several different C18 chemistries enable chromatographers to

choose the specific column for their application requirements.
Each phase provides a slightly different selectivity to help fine-tune
difficult separations.
Cyano-UG is extremely pH stable

Combi-RP (C30) columns are specially packed for high
flow rate Combinatorial Chemistry Applications
C18, C8, Phenyl and Cyano-UG Series provide excellent
peak shape for acidic, basic and chelating compounds
Combi-RP (Patented)
Combi-RP is a C30 chemistry engineered to provide a different
selectivity compared to C18 columns with added polar compound
selectivity and 100 % water compatibility.
Under 100 % aqueous conditions, conventional phases such as C18
collapse and fold on the silica gel surface in an effort to escape from
water. Additionally, column lifetime decreases under 100% aqueous
Under 100 % Aqueous Conditions
mobile phase conditions. For these reasons, C18 columns are intended for use with >10 % organic in the mobile phase.
The lifetime of some conventional C18 columns may greatly decrease
when the organic content of the mobile phase is less than 10 %. Develosil Combi-RP is very stable because of the long C-30 ligand, and
can be used from pH 2 to pH 8 under 100 % aqueous conditions.
The Develosil C18(ODS) Column Shows Only One Peak,

Combi-RP C-30 Separates 5 Peaks!
Develosil C18 (ODS)

Initial
Short retention and low

reproducibility
vs.
After 35 hours
Combi-RP C30
Long retention and no

change of retention under
100 % aqueous condition
App ID 13582
Develosil | HPLC
Decreased retention
Combi-RP C30
App ID 13581
Develosil C18 (ODS)
Dimensions: 50 x 4.6 mm
Mobile Phase: A) Buffer (pH 7.0)
B) Acetonitrile
Gradient: C18 column:
10 % B to 100 % B in
5 minutes
Combi-RP:
0 % B to 100 % B in
5 minutes
Flow Rate: 3.0 mL/min
Detection: UV @ 254 nm
Sample: Nucleic Acid Bases
RP-Aqueous and RP-Aqueous-AR (Patented C30 Phases)

For difficult polar compounds
Use AR phase for improved acid resistance
Fructo-Oligosaccharides
Column:
Dimensions:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample volume:
Sample:
Develosil 5 m RP-Aqueous
250 x 4.6 mm
CHO-5690
0.1 M K2HPO4 with KOH, pH 6.0
1.0 mL/min
30 C
UV @ 260 nm
4 L
1. 5'-CTP
6. 5'-GMP
2. 5'-CDP
7. 5'-ATP
3. 5'-CMP
8. 5'-ADP
4. 5'-GTP
9. 5'-AMP
5. 5'-GDP
App ID 13551
Column:
Dimensions:
Part Number:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample volume:
Sample:
164
Develosil 5 m RP-Aqueous
250 x 4.6 mm
CHO-5690
Water
1.0 mL/min
30 C
RI
10 L
1. Glucose
2. Saccharose
3. 1-Kestose
4. Nystose
5. 1F-Fructofuranosylnystose
App ID 13555
Nucleotides
Phenomenex
Develosil
By Nomura Chemical Co.

Develosil Columns
Part No.
Mfr. No.
3 m ODS-UG (C18)
CH0-3506
UG11346050W
CH0-3406
UG11346075W
CH0-3407
UG11346100W
CH0-3408
UG11346150W
5 m ODS-UG (C18)
CH0-3379
UG11546150W
CH0-3380
UG11546250W
CH0-3384
UG11540010W
5 m C8-UG (C8)
CH0-4221
UG12546150W
CH0-4222
UG12546250W
5 m CN-UG (Cyano)
CH0-4245
UG16546150W
CH0-4246
UG16546250W
CH0-4250
UG16540010W
5 m TMS-UG (C1)
CH0-4228
UG14546100W
CH0-4229
UG14546150W
CH0-4230
UG14546250W
CH0-4234
UG14540010W
5 m ODS-MG
CH0-4469
CH0-4470
CH0-4477
MG11546150W
MG11546250W
MG11540010W
Phenomenex
Size (mm)
ODS-UG-3
ODS-UG-3
ODS-UG-3
ODS-UG-3
50 x 4.6
75 x 4.6
100 x 4.6
150 x 4.6
ODS-UG-5
ODS-UG-5
ODS-UG-5 (Guard
Cartridge)
150 x 4.6
250 x 4.6
10 x 4.0
C8-UG-5
C8-UG-5
150 x 4.6
250 x 4.6
CN-UG-5
CN-UG-5
CN-UG-5 (Guard
Cartridge)
150 x 4.6
250 x 4.6
10 x 4.0
TMS-UG-5
TMS-UG-5
TMS-UG-5
TMS-UG-5 (Guard
Cartridge)
100 x 4.6
150 x 4.6
250 x 4.6
10 x 4.0
ODS-MG-5
ODS-MG-5
ODS-MG-5 (Guard
Cartridge)
150 x 4.6
250 x 4.6
10 x 4.0
ODS-HG-3
ODS-HG-3
ODS-HG-3
50 x 4.6
100 x 4.6
150 x 4.6
ODS-HG-5
ODS-HG-5
150 x 4.6
250 x 4.6
Combi-RP-3
Combi-RP-3
35 x 3.0
50 x 4.6
Combi-RP-5
Combi-RP-5
50 x 4.6
50 x 20.0
Price
Develosil Columns (continued)

Part No.
Mfr. No.
3 m RP-Aqueous
CH0-5991
RPAQ310100W
CH0-5992
RPAQ310150W
CH0-5996
RPAQ320050W
CH0-5998
RPAQ320150W
CH0-5999
RPAQ330050W
CH0-6001
RPAQ330150W
CH0-6002
RPAQ346035W
CH0-6003
RPAQ346050W
CH0-6006
RPAQ346150W
5 m RP-Aqueous
CH0-5683
RPAQ520050W
CH0-5685
RPAQ520150W
CH0-5686
RPAQ520250W
CH0-5687
RPAQ546050W
CH0-5689
RPAQ546150W
CH0-5690
RPAQ546250W
CH0-5695
RPAQ5P1250W
CH0-5699
RPAQ5P2050W
CH0-5696
RPAQ5P2250W
CH0-5672
UG17540010W
Description
Size (mm)
RP-AQUEOUS-3
RP-AQUEOUS-3
RP-AQUEOUS-3
RP-AQUEOUS-3
RP-AQUEOUS-3
RP-AQUEOUS-3
RP-AQUEOUS-3
RP-AQUEOUS-3
RP-AQUEOUS-3
100 x 1.0
150 x 1.0
50 x 2.0
150 x 2.0
50 x 3.0
150 x 3.0
35 x 4.6
50 x 4.6
150 x 4.6
RP-AQUEOUS-5
RP-AQUEOUS-5
RP-AQUEOUS-5
RP-AQUEOUS-5
RP-AQUEOUS-5
RP-AQUEOUS-5
RP-AQUEOUS-5
RP-AQUEOUS-5
RP-AQUEOUS-5
RP-AQUEOUS (Guard
Cartridge)
50 x 2.0
150 x 2.0
250 x 2.0
50 x 4.6
150 x 4.6
250 x 4.6
250 x 10.0
50 x 20.0
250 x 20.0
10 x 4.0
3 m RP-Aqueous-AR
CH0-7393
RPAR310050W
RP-AQUEOUS-AR-3
50 x 1.0
CH0-7394
RPAR310150W
RP-AQUEOUS-AR-3
150 x 1.0
CH0-7395
RPAR320150W
RP-AQUEOUS-AR-3
150 x 2.0
CH0-7409
RPAR346050W
RP-AQUEOUS-AR-3
50 x 4.6
CH0-7396
RPAR346100W
RP-AQUEOUS-AR-3
100 x 4.6
CH0-7397
RPAR346150W
RP-AQUEOUS-AR-3
150 x 4.6
5 m RP-Aqueous-AR
CH0-7399
RPAR510150W
RP-AQUEOUS-AR-5
150 x 1.0
CH0-7401
RPAR520150W
RP-AQUEOUS-AR-5
150 x 2.0
CH0-7403
RPAR546150W
RP-AQUEOUS-AR-5
150 x 4.6
CH0-7404
RPAR546250W
RP-AQUEOUS-AR-5
250 x 4.6
CH0-7405
RPAR540010W
RP-AQUEOUS-AR-5
10 x 4.0
5 m 300 ODS-HG (Widepore C18)
CH0-3396
HG31546250W
300ODS-HG-5
250 x 4.6
Guard Cartridges for UG and HG columns
CH0-3417
UG11540010C
ODS-UG-5 (4/pk.)
10 x 4.0
Guard Cartridge Holder for UG, HG, and RP-Aqueous columns
CH0-3419
HO00040010C
Guard Cartridge Holder
10 x 4.0
Price
HPLC | Develosil
3 m ODS-HG (C18)
CH0-3411
HG11346050W
CH0-3413
HG11346100W
CH0-3414
HG11346150W
5 m ODS-HG (C18)
CH0-3387
HG11546150W
CH0-3388
HG11546250W
3 m COMBI-RP
CH0-5893
COMB330035W
CH0-5896
COMB346050W
5 m COMBI-RP
CH0-5902
COMB546050W
CH0-5904
COMB5P2050W
Description
165
EnviroSep
If the results obtained with an EnviroSepPP or EnviroSep-CM analytical column do

not meet your separation requirements
or do not provide you with a least
equivalent separations as compared to
competing HPLC columns of comparable
dimensions, return the column with
full refund.
Columns for Environmental

Analysis
EnviroSep-ABC-GPC sample cleanup column
EnviroSep-CM-Carbamate analysis column (EPA Method 632)
Synergi Hydro-RP-Explosives analysis (EPA Method 8330)
EnviroSep-PP-PAH analysis column (EPA Method 610)
PAH with Surrogate Standards on EnviroSep-PP (EPA Method 610)
EnviroSep-PP
Sample Analysis of Priority Pollutants
Column:
Dimensions:
Part No.:
Flow Rate:
Mobile Phase:
EnviroSep-PP
125 x 4.6 mm
00E-3029-E0
2.0 mL/min
A: Water
B: Acetonitrile. Equilibrate column with (60:40) A/B, run isocratic
under this condition for 2 minutes following injection.
Then run a linear gradient to 100 % B in 25 minutes.
Detection: A: UV @ 254 nm
B: Programmed fluorescence; 0 minutes Ex: 240 nm Em: 425 nm;
16 minutes Ex: 254 nm Em: 395 nm
Sample: 16 component PAH mixture with 3 surrogates, 20 L
Peaks: 1. Nitrobenzene*
10. p-Terphenyl*
2. Naphthalene
3. Acenaphthylene 12. Chrysene
4. Acenaphthene
13. Benzo[e]pyrene*
5. Fluorene
6. Phenanthrene
7. Anthracene
16. Benzo[a]pyrene
8. Fluoranthene
9. Pyrene
19. Indeno(1,2,3-cd)pyrene
*Surrogate Standards
Polymeric bonded phase excellent for PAH

analysis (EPA Method 610)
Besides PAHs, EnviroSep-PP columns can also resolve
phenols, triazines, phthalate esters and pesticides.
Envirosep-PP Applications
App. ID
Benzidines on EnviroSep-PP (EPA 605)*

Phenols on EnviroSep-PP (EPA 604)*
Phthalate Esters on EnviroSep-PP (EPA 606)*
Carbamate/Urea Pesticides on EnviroSep-PP (EPA 632)**
Herbicides**
PAH with Surrogate Standards on EnviroSep-PP (Fluor)**
PAH with Surrogate Standards on EnviroSep-PP (UV)**
Phenols**
Phthalates**
5302
5304
5303
5301
13239
5300
5299
13243
13242
* Dimensions: 125 x 3.2 mm ** Dimensions: 125 x 4.6 mm

1000
A. (UV)
900
800
700
EnviroSep-CM
600
500
Carbamate Analysis
400
14
300
Response (mV)
This column shows excellent resolution and separation times

of carbamate pesticides under easy-to-use mobile phase
conditions.
3
2
200
10
12
11 13 1516
10
15
19
18
17
100
App ID 5299
Envirosep | HPLC
Application Title
0
0
20
25
30 min
Carbamates on EnviroSep-CM (EPA Method 632)
166
10
12
15
1000
B. (Fluorescence)
900
16
800
700
7
600
11
500
400
15
13
14
14
16
12
18
14
13
17
App ID 5300
10
300
Response (mV)
Flow Rate:
Temperature:
Detection:
Sample:
EnviroSep-CM
175 x 3.2 mm
00V-3133-R0
A: Water B: Acetonitrile. Equilibrate column with A/B (82:15), run
a gradient following injection to 50 % B in 14 minutes
1 mL/min
45 C
UV @ 254 nm
Carbamate mixture 15 L
4
1. Oxamyl
10. Propham
2. Methomyl
11. Siduron
12
3. Fenuron
12. Linuron
6
89
4. Monuron
13. Chlorpropham
11
1
3
5. Propoxur
14. Barban
7
6. Carbofuran 15. Neburon
5
7. Carbaryl
2
8. Fluometuron
10
9. Diuron
App ID 5305
Column:
Dimensions:
Part No.:
Mobile Phase:
25
30 min
2
45
200
100
0
0
10
15
20
18 min
Phenomenex
EnviroSep
EnviroSep- ABC
EPA Calibration Standard for GPC Using

EnviroSep-ABC High Efficiency Columns
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Sample:
Fast sample processing (< 30 minutes)

Substantial reduction in solvent consumption
(up to 50 %)
Very high loading capacity, with >85 % lipid
removal as per EPA method requirements
EnviroSep-ABC size exclusion columns separate environmental
samples on the basis of molecular size and provide a rapid, reliable
and automated mechanism for the removal of lipids, polymers,
resins and other high molecular weight compounds that interfere
with GC/MS analysis of organic pollutants.
EnviroSep-ABC + EnviroSep-ABC Guard

350 x 21.2 mm + 60 x 21.2 mm (Guard)
See ordering information
Methylene Chloride
5 mL/min
UV @ 254 nm
Injected 5 mL EPA calibration
standard containing:
1. Corn oil, 125 mg
2. Diethylhexylphthalate, 5 mg
3. Methoxychlor, 1 mg
3
4. Perylene, 0.1 mg
5. Sulfur, 0.4 mg
EnviroSep-ABC
Phenomenex Part No.
03R-3035-P0
00W-3035-P0
03B-3035-K0
00H-3035-K0
Size (mm)
30 x 3.2 (Guard)
30 x 4.6 (Guard)
125 x 2.0
125 x 3.2
125 x 4.6
ABC Lab Part No.

417-250
417-150
417-200
417-151
Description
Preparative Guard
Preparative Column
Analytical Guard
Analytical Column
EnviroSep-ABC GPC Standard Tested Columns*

Phenomenex Part No.
ABC Lab Part No.
Description
00W-3035-P0-GP
Preparative Column
00H-3035-K0-GP
Analytical Column
*GPC tested columns use the EPA standard test mixture (see App ID 5307 above)
and should be used for methods specifying minimum resolution requirements.
For Column Heaters, see p. 357.
Phenomenex
EnviroSep-CM
Part No.
03A-3133-R0
00V-3133-R0
00V-3133-E0
Price
Size (mm)
60 x 21.2
350 x 21.2
50 x 7.8
300 x 7.8
Price
Size (mm)
350 x 21.2
300 x 7.8
Price
20
Size (mm)
30 x 3.2 (Guard)
175 x 3.2
175 x 4.6
30 min
Price
HPLC | Envirosep
EnviroSep-PP
Part No.
03A-3029-R0
03A-3029-E0
00E-3029-B0
00E-3029-R0
00E-3029-E0
10
App ID 5307
Columns for GPC Cleanup
Request Technical Note, TN-2045, for GPC Cleanup

using EnviroSep-ABC
167
Gemini
U.S. Patent No. 7, 563, 367
If Gemini analytical columns do not

provide at least an equivalent separation
as compared to a competing column of
the same particle size, similar phase
and dimensions, return the column with
full refund.
Setting the Standard for pH

Method Development
Rugged reversed phase HPLC columns that offer extended lifetime
at extreme pH conditions and excellent stability for reproducible,
high efficiency separations.
Take full advantage of high and low pH conditions
(pH 1-12) to manipulate selectivity
Expect longer column lifetime with patented TWIN-NX
technology
For analytical and preparative separations of basic and
acidic compounds
Description
NX C18
The most rugged Gemini column, offering 5 times the durability of

previous generation hybrid columns
A low bleed phenyl phase. For UV and MS detection, which offers
an aromatic selectivity complementary to C18 phases
Selectivity, high structural integrity and increased loadability for
preparative and purification applications in pre-packed columns
and bulk media
C6-Phenyl
SFC
Gemini | HPLC
APPROVED
USP
Classification
Phase
C18
L1
L11
L1
TWIN (Two-In-One) Technology
Second-Generation TWIN-NX
Technology
During the final stage of silica manufacturing a unique silica-organic

layer is grafted to create a completely new composite particle.
Since the internal base silica is unaltered by this manufacturing process, the particle retains its mechanical strength and rigidity along
with excellent efficiency, while the silica-organic shell protects the
particle from chemical attack.
TWIN-NX technology uses an improved patented organo-silica

grafting process which incorporates highly stabilizing ethane crosslinking. These organic groups are evenly incorporated into the grafted
layers on the silica surface while maintaining a pure silica core. This
not only provides resistance to high pH attack, but also maintains the
high efficiency and mechanical strength of a silica particle.
OH
OH
OH
R
OH
Si
O
R
Si
Si
Si
O
O
Si
Si
Si
Si
O
O
Si
Si
Si
Si
Si
Si
Si
Si
Si
Si
Si
Si
Si
Si
Si
OH
Si
Si
Si
Si
Si
Si
O
O
O
O
CH
CH
O
Si
O
Si
CH CH S
CH CH Si
Si
O
Si
Si
O
Si
CH
CH
O
O
O
O
O
O
O
O
C
CH
CH
O
Si
O
Si
CH
CH CH Si
CH CH Si
O
Si
O
Si
Si
CH Si
C
CH
O
O
O
O
O
O
O
O
C
CH
CH
CH
Si
O SiCH
Si
O
SiCH
CH
CHSi
O Si
Si
O
Si
CH
CH
O
O
O
O
O
O
O
Si
O
O
Si
Si
O
O
Si
Si
Si
Si
O
O
O
O
Si
Si
O
O
Si
O
O
Si
O
O
Si
O
O
O
Si
Si
Si
Si
O
Si
Si
O
O
O
Si
Si
Si
O
O
Si
Si
O
O
Si
Si
Si
Si
O
O
Si
O
O
O
O
Si
O
O
Si
Si
O
O
O
Si
O
O
O
O
O
O
OH Si
Si
O
O
Si O Si
O
OH
Si
Si
O Si
OH
Si
Si
Si
OH
OH
OH
OH
Si
Si
OH
OH
O
O
Si
O
Si O Si O Si O Si O Si O Si O Si O Si
168
Phenomenex
Gemini
U.S. Patent No. 7, 563, 367
Gemini-NX C18
Gemini-NX C18
pH Stability:
Particle Size:
Phase:
Application:
Strength:
Control selectivity of ionizable compounds for

optimized methods
Consistent performance in both volatile and non-volatile
buffers
USP: L1
LC/MS
Certified
1.0 12.0
3 m, 5 m, and 10 m
C18
Small molecules, basic compounds
Most durable hybrid particle
High sample loading capacity for metabolite identification

and preparative purification
pH stable 1-12 for durability
Packing Material
Particle
Shape/Size
(m)
Pore
Size
()
Surface
Area
(m2/g)
Carbon
Load
%
End
Capping
Gemini-NX C18
Spherical 3, 5, 10
110
375
14
TMS
Step 1
24x High pH Flush Procedures
Gemini-NX Tested for Extreme Durability in

Changing Mobile Phase pH
Column Efficiencies Maintained in High pH Testing for 20 Cycles
110
Step 2
High pH Testing
90
70
1x Neutral Flush Procedure

Mobile Phase: A: Water
B: Acetonitrile
Gradient: 5 % B for 2 min
5 % to 100 % B in 3 min Hold at 100 % B
for 5 min
50
0
Diphenhydramine
10
15
20
Cycles of Exposure
HPLC | Gemini
Step 3
Stable Efficiencies After Exposure to

10,000
Column Volumes of High and Low pH
Buffers
80
60
Step 4
Neutral pH Testing
Tetracaine
Isocratic:
Flow Rate:
Detection:
Samples:
Retention Times of Four Probes Maintained in Neutral pH

Testing for 20 Cycles
6
Water / Acetonitrile (35:65)

1.0 mL/min
UV @ 254 nm
1. Acetophenone
2. Benzene
3. Toluene
4. Acenaphthene
Step 5
Retention in Minutes
SFC
APPROVED
Isocratic: 10 mM Ammonium Bicarbonate pH 10.5 /

Acetonitrile (50:50)
Samples: 1. Tetracaine
2. Diphenhydramine
100
% of Initial Efficiencies
Mobile Phase: A: 10 mM Ammonium Bicarbonate pH 10.5

B: Acetonitrile
Gradient: 5 % to 95 % B in 6 min Hold at 95 % B
for 2 min
Re-equilibrate: 5 % B for 2 min
24x Low pH Flush Procedure

Mobile Phase: A: 0.5 % Formic Acid in Water
B: 0.5 % Formic Acid in Acetonitrile, pH 2.0
Gradient: 5 % to 95 % B in 6 min
Hold at 95 % B for 2 min
Re-equilibrate: 5 % B for 2 min
Step 6
Retention Times Maintained After Exposure

to 10,000 Column Volumes of High and Low
pH Buffers
Neutral pH Flush Repeats

Repeats for 20 Cycles
0
0
10
15
20
Sequence
Acetophenone
Benzene
Phenomenex
Toluene
Acenaphthene
Column Used:
Column: Gemini-NX 5 m C18
Part No.: 00F-4454-E0
169
Gemini
U.S. Patent No. 7, 563, 367

Polar Bases at High pH, pH 10.5
Gemini-NX 5 m C18
Waters XBridge 5 m C18
2
3
}
0
10
10
20
App ID 16641
4,5
10
min
10
App ID 16642
20
min
Y-axis normalized for all chromatograms.
Polar Bases (Beta Blockers) at High pH

Mobile Phase: A: 10 mM Ammonium Bicarbonate pH 10.5
B: Acetonitrile
Gradient: A/B (85:15) to (70:30) in 15 min to (50:50) in
5 min, Hold for 5 min
Temperature: Ambient
SFC
Polar Bases at Low pH, pH 2.7
Gemini-NX 5 m C18
7
6
1 34
2
Waters XBridge 5 m C18
6
7
App ID 16606
1,2 3 4
App ID 16633
Gemini | HPLC
APPROVED
Sample: 1. Bisoprolol Contaminant

2. Sotalol
3. Atenolol
4. Labetalol (Diastereoisomeric Pair)
5. Nadolol (Diastereoisomeric Pair)
6. Pindolol
7. Metoprolol
8. Bisoprolol
9. Propranolol
10. Alprenolol
10
12 min
10
12 min
Y-axis normalized for all chromatograms.
Polar Bases (Antihistamines) in Formic Acid

Mobile Phase: A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Acetonitrile
Gradient: A/B (90:10) to (50:50) in 10 min
Sample: 1.
2.
3.
4.
5.
6.
7.
Pyrilamine
Tripelennamine
Chlorpheniramine
Brompheniramine
Chloropyramine
Diphenhydramine
Loratadine
Comparative chromatograms may not be representative of all applications.
170
Phenomenex
Gemini
U.S. Patent No. 7, 563, 367
Gemini C18
Gemini C18
pH Stability:
Particle Size:
Phase:
Application:
Strength:
Increased loading and retention of basic compounds

Wide pH stability from 1-12
Silica efficiency and mechanical strength
USP: L1
LC/MS
Certified
1.0 12.0
3 m, 5 m, and 10 m
C18
Wide pH stability, high efficiency
Excellent media for process-scale purification
Packing Material
Particle
Shape/Size
(m)
Pore
Size
()
Surface
Area
(m2/g)
Carbon
Load
%
End
Capping
Gemini C18
Spherical 3, 5, 10
110
375
14
TMS
Batch-to-Batch Reproducibility
pH Quick Guide
SFC
Effects of Mobile Phase pH on Acids
Low pH
Batch 4
Batch 3
Batch 2
At pKa
High pH
Below pKa
HPLC | Gemini
App ID 15818
Acids
APPROVED
Above pKa
Batch 1
4
min
Conditions for all separations:

Column: Gemini 5 m C18
Dimension: 150 x 4.6 mm
Part No.: 00F-4435-E0
Mobile Phase: 10 mM Ammonium Bicarbonate,
pH 10.5/Acetonitrile (50:50)
Sample: 1. Pindolol
2. Metoprolol
3. Propranolol
Phenomenex
Effects of Mobile Phase pH on Bases
Low pH
Bases
Below pKa
At pKa
High pH
Above pKa
171
Gemini
U.S. Patent No. 7, 563, 367
Virtually No LC/MS Bleed
Extended Column Lifetime
The increased stability of Gemini columns provide the ultra-low

bleed profile required for high sensitivity LC/MS applications. The
advanced Twin Technology used for Gemini columns limit phase
cleavage and stabilizes the silica surface. When compared against
a background MS profile, Gemini columns demonstrate virtually no
column bleed across a wide mass range.
The Twin Technology engineering of Gemini provide stability and

increased column lifetime. Whether used under isocratic or gradient conditions, Gemini columns out-perform and outlasts pH stable
columns. This is illustrated below.
Total Ion Current
Lifetime and Efficiency Comparison**
9.0e7
Essentially
no bleed!
8.0e7
7.0e7
5.5 times increased column

lifetime and still going!
16000
14000
6.0e7
12000
5.0e7
Efficiency (N)
10000
4.0e7
3.0e7
2000
0.0
1
10

Column: Gemini 5 m C18
Dimension: 150 x 3.0 mm
Part No.: 00F-4435-Y0
Mobile Phase: A: 0.1% HCOOH in Acetonitrile
B: 0.1% HCOOH in Water
Gradient: 5:95 (A/B) linear to 95:5 over 8 min,
Hold for 5 min
Detection: API 3000
Ion Source:ESI/PIM
Scan Rate:13000 m/z/s
Scan Range:60-1000
11
12
min
Gemini C18
Background MS Profile
0
1
10
13
16
19
22
25
28
31
34
37
40
43
46
49
Days
Efficiency
and lifetime comparison based on average of two

columns each run in parallel.
Gemini C18
Waters XTerra MS C18

Columns: Gemini 5 m C18
Waters XTerra 5 m MS C18
Mobile Phase: Acetonitrile/50 mM Methylpyrrolidine
Buffer, pH 11.5 (50:50)
Flow Rate: 1 mL/min
Sample: Diphenhydramine
Chromatographic Comparisons**
Gemini 5 m C18 110
15595
10
12
14 min
2
3
15602
10
12
14 min
Advanced Chromotography Technologies

ACE 5 m C18 100
Tricyclic Antidepressants at Neutral pH
2
3
4
App ID 15602

Mobile Phase: 20 mM Phosphate buffer pH 7.0/Acetonitrile/
Methanol (30:35:35)
Sample: 1. Nortriptyline
2. Imipramine
3. Amitriptyline
4. Clomipramine
App ID 15596
Virtually no silanol
interactions!
Zorbax 5 m Extend-C18 80
15596
App ID 15595
Gemini | HPLC
XTerra Fail Line
Columns fail after 50%

loss in efficiency.
4000
1.0e7
SFC
Gemini Fail Line
6000
2.0e7
APPROVED
8000
10
12
14 min
**The comparative data presented here may not be representative for all applications.
Gemini is a registered trademark of Phenomenex, Inc.
Twin and Two-In-One Technology are trademarks of Phenomenex, Inc.
Waters and XTerra are registered trademarks of Waters Corp.
Zorbax is a registered trademark of Agilent Technologies.
ACE is a registered trademark of Advanced Chromatography Technologies.
Phenomenex is not associated in any way with Waters Corp, Agilent Technologies or ACT.
172
Phenomenex
Gemini
U.S. Patent No. 7, 563, 367
Gemini C6-Phenyl
Gemini C6-Phenyl
pH Stability:
Particle Size:
Phase:
Application:
Strength:
Good selectivity for aromatic compounds

Extremely low UV and MS bleed
Wide pH stability from 1-12
LC/MS
Certified
USP: L1
1.0 12.0
3 m, 5 m, and 10 m
C18
High aromatic selectivity with exceptional peak
shape even in neutral conditions. Extremely low
bleed phenyl column.
Packing Material
Particle
Shape/Size
(m)
Pore
Size
()
Surface
Area
(m2/g)
Carbon
Load
%
End
Capping
Gemini C6-Phenyl
Spherical 3, 5
110
375
12
TMS
Reproducible Phenyl Phase

Aliphatic Acid Application
SFC
Enhanced Performance
for Aromatic Compounds
1
min
Sulfa Drug Application

RESOLUTION
Pursuit 5 m DiPhenyl
Gemini 5 m C6-Phenyl
1.0
9.8
4.0
16.0
Rs1,2
Rs2,3
Phenomenex Gemini 5 m C6-Phenyl

mAU
1 2
120
80
40
HPLC | Gemini

Column: Gemini 5 m C6-Phenyl
Part No.: 00F-4444-E0
Mobile Phase: 20 mM Phosphate buffer, pH 2.5/
Methanol (97:3)
Sample: 1. Tartaric Acid 4. Acetic Acid
2. Malic Acid
5. Citric Acid
3. Lactic Acid
6. Propionic Acid
App ID 15903
APPROVED
Low Bleed Phenyl Phase
10
min
mAU
60
40
20
0
Intensity cps
9.0e7
10
15
min
Popular C18
8.0e7

Mobile Phase: 0.1 % Formic Acid in Water/
Methanol (70:30)
Sample: 1. Sulfathiazole
2. Sulfamerazine
3. Sulfamethoxazole
7.0e7
6.0e7
5.0e7
Gemini C6-Phenyl
4.0e7
3.0e7
2.0e7
Blank
Sulfathiazole
10
12 min

Mobile Phase: A: 0.1 % Formic acid in Water
B: 0.1 % Formic acid in Acetonitrile
Gradient: 5 % B to 95 % B in 10 min, then hold
95 % B for 2 min
MS Detection: ESI + ion mode, M/Z 100-700
Phenomenex
O
S
1.0e7
0.0
15
Varian Pursuit 5 m DiPhenyl
App ID 15908
One of the weaknesses of traditional phenyl phases has been

excessive bleed in UV and MS. Gemini C6-Phenyl columns
demonstrate far lower bleed than many other phenyl columns.
Indeed, in this example the Gemini C6-Phenyl column shows
lower bleed than a popular C18 column.
S
N
H
H2N
Sulfamerazine
O
S
N
H
CH3
H2N
The comparative data presented here may not be representative for all applications.
Pursuit is a registered trademark of Varian, Inc.
173
Gemini
U.S. Patent No. 7, 563, 367
Applications
Local Anesthetics
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:
Caffeine and Metabolites

Column:
Dimensions:
Part No.:
Mobile Phase:
Gemini 5 m C18
150 x 4.6 mm
00F-4435-E0
10 mM NH4HCO3, pH 10.5 / Acetonitrile (50:50)
1 mL/min
25 C
UV @ 210 nm
1. Procaine
2. Lidocaine
3. Tetracaine
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:
15014
14999
Gemini 5 m C18
150 x 4.6 mm
00F-4435-E0
A: 20 mM Ammonium acetate, pH 7.5
B: Acetonitrile
98:2 (A/B) linear to 80:20 over 15 min, Hold for 3 min
1 mL/min
25 C
UV @ 273 nm
1. Xanthine
3. Theophylline
2. Theobromine 4. Caffeine
2
3
4
App ID 14999
SFC
APPROVED
10
12
14 min
10
15
min
Clinical Solution
Benzodiazepines in Plasma
Flavonoids
SPE Conditions
Sample: 0.5 mL Human Plasma + 0.5 mL Water + 0.1 mL std
(2.0 g/mL in Water)
Sorbent: Strata-X 30 mg/1 mL tubes
Part No.: 8B-S100-TAK
Condition: 1 mL Methanol followed by 1 mL Water
Wash: 1 mL Water, 1 mL of 40 % Methanol
Elution: 1 mL Methanol, evaporated to dryness under gentle N2 flow
Reconstitute: with 100 L water
HPLC Conditions
Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Sample:
Gemini 5 m C18
150 x 3.0 mm
00F-4435-Y0
A: 0.1 % Formic acid in Water
80:20 (A/B) linear to 20:80 over 8 min, Hold for 1 min
0.8 mL/min
22 C
UV @ 254 nm
1. Chlordiazepoxide
4. Clonazepam
2. Flurazepam
5. Temazepam
3. Oxazepam
6. Diazepam
150 x 4.6 mm
00F-4444-E0
0.1 % Formic acid in Water/Methanol (45:55)
1 mL/min
UV @ 254 nm
1. Quercetin
2. Kaempferol
3. Isorhamnetin
15895
1
mAU
70
60
50
40
App ID 15895
Gemini | HPLC
App ID 15014
30
2
20
10
0
0
10
12
14
16
18
min
4 5 6
Benzodiazepines spiked
in human plasma
2 g/mL benzodiazepine
standard
Human
plasma blank
174
10
12
App ID 15454, 15455, 15456
3
2
min
Phenomenex
If Gemini analytical columns do not provide at least an equivalent

separation as compared to a competing column of the same
particle size, similar phase and dimensions, return the column
with comparative data within 45 days for a full refund.
Gemini
U.S. Patent No. 7, 563, 367

3 m Microbore, Minibore and Narrow Bore Columns (mm)
Phases
50 x 1.0
20 x 2.0
30 x 2.0
C18
00B-4439-A0
00M-4439-B0
00A-4439-B0
00B-4439-B0
00D-4439-B0
00F-4439-B0
00B-4439-Y0
00D-4439-Y0

150 x 3.0
4 x 2.0*
/10pk
00F-4439-Y0
AJ0-7596
C6-Phenyl
00B-4443-A0
00A-4443-B0
00B-4443-B0
00D-4443-B0
00F-4443-B0
00B-4443-Y0
00D-4443-Y0
00F-4443-Y0
00M-4453-B0
00A-4453-B0
00B-4453-B0
00D-4453-B0
00F-4453-B0
00B-4453-Y0
00D-4453-Y0
00F-4453-Y0
NX C18
3 m Analytical Columns (mm)

Phases
20 x 4.0
C18
C6-Phenyl
NX C18
00M-4439-D0
50 x 2.0
100 x 2.0
150 x 2.0
50 x 3.0
100 x 3.0
30 x 4.6
50 x 4.6
100 x 4.6
150 x 4.6
00A-4439-E0
00A-4443-E0
00B-4439-E0
00B-4443-E0
00D-4439-E0
00D-4443-E0
00F-4439-E0
00F-4443-E0
00B-4453-E0
00D-4453-E0
00F-4453-E0
5 m Minibore and Narrow Bore Columns (mm)

Phases
30 x 2.0
50 x 2.0

250 x 4.6
4 x 3.0*
/10pk
00G-4439-E0
AJ0-7597
00G-4443-E0
AJ0-7915
/10pk
00G-4453-E0
AJ0-8368
for ID: 3.2-8.0 mm
150 x 2.0
250 x 2.0
50 x 3.0
100 x 3.0
150 x 3.0
00G-4435-B0
00B-4435-Y0
00B-4444-Y0
00D-4435-Y0
00F-4435-Y0
00F-4444-Y0
00B-4454-Y0
00D-4454-Y0
00F-4454-Y0
C18
C6-Phenyl
00A-4435-B0
00A-4444-B0
00B-4435-B0
00B-4444-B0
00F-4435-B0
00F-4444-B0
NX C18
00A-4454-B0
00B-4454-B0
00F-4454-B0

Phases
30 x 4.6

50 x 4.6
100 x 4.6
150 x 4.6
250 x 4.6
4 x 3.0*
/10pk
C18
00A-4435-E0
00B-4435-E0 00D-4435-E0 00F-4435-E0 00G-4435-E0
AJ0-7597
C6-Phenyl
00A-4444-E0
00B-4444-E0 00D-4444-E0 00F-4444-E0 00G-4444-E0
AJ0-7915
/10pk
NX C18
00B-4454-E0 00D-4454-E0 00F-4454-E0 00G-4454-E0

AJ0-8368
for ID: 3.2-8.0 mm
5 m Semi-Prep Columns (mm)
Phases
150 x 10
250 x 10
10 x 10
/3pk
C18
00F-4435-N0
00G-4435-N0
AJ0-7598
C6-Phenyl
00G-4444-N0
AJ0-7314
/3pk
NX C18
00F-4454-N0
00G-4454-N0
AJ0-8369
for ID: 9-16 mm
Axia Packed Preparative Columns (mm)
Phases
50 x 21.2
100 x 21.2
150 x 21.2
250 x 21.2
50 x 30
5 m
C18
00B-4435-P0-AX
00D-4435-P0-AX
00F-4435-P0-AX
00G-4435-P0-AX
00B-4435-U0-AX
C6-Phenyl
00D-4444-P0-AX
00F-4444-P0-AX
00G-4444-P0-AX
5 m
NX C18
00B-4454-P0-AX
00D-4454-P0-AX
00F-4454-P0-AX
00G-4454-P0-AX
00B-4454-U0-AX
10 m
C18
00B-4436-P0-AX
00D-4436-P0-AX
00F-4436-P0-AX
00G-4436-P0-AX
00B-4436-U0-AX
10 m
NX C18
00B-4455-P0-AX
00D-4455-P0-AX
00F-4455-P0-AX
00G-4455-P0-AX
250 x 30
aufInquire
Anfrage
00G-4435-U0-AX
00G-4444-U0-AX
aufInquire
Anfrage
00G-4454-U0-AX
aufInquire
Anfrage
00G-4436-U0-AX
aufInquire
Anfrage
00G-4455-U0-AX
50 x 50
aufInquire
Anfrage
00B-4435-V0-AX
aufInquire
Anfrage
00B-4436-V0-AX
aufInquire
Anfrage
00B-4455-V0-AX
For PREP Columns & Bulk Media, see p. 335

For SecurityGuard Holders and Cartridges, see p. 235
For MercuryMS LC/MS Columns, Cartridges, and Cartridge Holders, Inquire.
Phenomenex
100 x 50
aufInquire
Anfrage
00D-4436-V0-AX
aufInquire
Anfrage
00D-4455-V0-AX

250 x 3.0
4 x 2.0*
/10pk
00G-4435-Y0
AJ0-7596
00G-4444-Y0
AJ0-7914
/10pk
00G-4454-Y0
AJ0-8367
for ID: 2.0-3.0 mm
SFC
APPROVED
For Gemini Capillary HPLC Columns,

Guards, and Adapter, contact your
Phenomenex technical consultant or
local distributor.
HPLC | Gemini
Axia Packed Preparative Columns (mm) continued

Phases
100 x 30
150 x 30
5 m
aufInquire
Anfrage
C18
00D-4435-U0-AX
00F-4435-U0-AX
C6-Phenyl
00D-4444-U0-AX
5 m
aufInquire
Anfrage
NX C18
00D-4454-U0-AX
00F-4454-U0-AX
10 m
aufInquire
Anfrage
C18
00D-4436-U0-AX
00F-4436-U0-AX
10 m
aufInquire
Anfrage
NX C18
00D-4455-U0-AX
00F-4455-U0-AX
AJ0-7914
/10pk
AJ0-8367
for ID: 2.0-3.0 mm
75 x 30
00C-4435-U0-AX
00C-4444-U0-AX
00C-4454-U0-AX
150 x 50
aufInquire
Anfrage
00F-4436-V0-AX
aufInquire
Anfrage
00F-4455-V0-AX

15 x 21.2**
15 x 30.0
/ea
/ea
AJ0-7846
AJ0-8308
AJ0-7841
AJ0-8303
/ea
/ea
AJ0-8370
AJ0-8371
/ea
/ea
AJ0-7846
AJ0-8308
/ea
/ea
AJ0-8370
AJ0-8371
for ID: 18-29 mm
30-49 mm
250 x 50
15 x 30.0
/ea
AJ0-8308
AJ0-8303
/ea
AJ0-8371
aufInquire
Anfrage
/ea
00G-4436-V0-AX
AJ0-8308
aufInquire
Anfrage
/ea
00G-4455-V0-AX
AJ0-8371
for ID: 30-49 mm

PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8277
175
Hamilton
By Hamilton Co.
Polymer-based materials
pH stable from 1 to 13
Pressure stable
300 columns for biopolymer separations
Hamilton
HPLC
Columns
Description
PRP-1 5 m
PRP-1 5 m
PRP-1 5 m PEEK
PRP-1 7 m
PRP-1 7 m PEEK
PRP-1 10 m
PRP-1 10 m PEEK
PRP-1 10 m
PRP-1 10 m
PRP-3 10 m 300 for Proteins and Peptides
PRP-3 10 m
PRP-3 10 m 300 PEEK
PRP-X100 10 m IC for Anion Analysis
PRP-X100 10 m
PRP-X100 10 m
PRP-X100 10 m
PRP-X100 10 m PEEK
PRP-X100 10 m PEEK
PRP-X100 10 m
PRP-X200 10 m for Cation Analysis
PRP-X200 10 m
PRP-X300 7 m Ion-Exclusion for Alcohols and Organic Acids
PRP-X400 7 m Ion-Exchange for Glyphosate Analysis
PRP-X400 7 m
PRP-Infinity 4 m Non-porous RP
HC-75 9 m Calcium Form for Carbohydrates
RCX-30 7 m PEEK Anion-Exchange for Complex Sugars
Guards and Starter Kits

Description
Guard Column Cartridge Holder
Guard Column Header Assembly
PRP-1 Guard Column Starter Kit
PRP-1 Guard Column Replacement Cartridges Kit (5/pk)
PRP-1 PEEK Guard Column Starter Kit
PRP-3 Guard Column Starter Kit
PRP-X100 Guard Column Starter Kit
PRP-X100 Guard Column Replacement Cartridges Kit (5/pk)
PRP-X100 PEEK Guard Column Starter Kit
PRP-X200 Guard Column Replacement Cartridges Kit (5/pk)
PRP-X400 PEEK Cartridge (5/pk)
RCX-10 Guard Column Starter Kit
RCX-10 Guard Column Replacement Cartridges Kit (5/pk)
Size(mm)
150 x 2.1
150 x 4.1
150 x 4.6
250 x 4.1
250 x 4.6
150 x 4.1
250 x 4.6
250 x 4.1
250 x 10
150 x 2.1
150 x 4.1
150 x 4.6
150 x 2.1
250 x 2.1
100 x 4.1
150 x 4.1
250 x 4.6
150 x 4.6
250 x 4.1
150 x 4.1
250 x 4.1
250 x 4.1
250 x 2.1
250 x 4.1
30 x 4.1
250 x 4.1
150 x 4.6
Part No.
CH0-4174
CH0-0677
CH0-1594
CH0-1650
CH0-3030
CH0-0678
CH0-4178
CH0-0679
CH0-1599
CH0-4179
CH0-0682
CH0-3031
CH0-4180
CH0-4181
CH0-0685
CH0-0686
CH0-3032
CH0-4182
CH0-0687
CH0-0688
CH0-0689
CH0-1169
CH0-4206
CH0-1175
CH0-1167
CH0-1629
CH0-5702
Mfr. No.
79366
79444
79423
79422
79380
79425
79381
79427
79496
79392
79466
79382
79421
79346
79439
79434
79455
79354
79433
79441
79442
79465
79398
79473
79470
79431
79370
Price
Part No.
CH0-1635
CH0-1636
CH0-0669
CH0-0670
CH0-1637
CH0-1639
CH0-0671
CH0-0672
CH0-4195
CH0-0673
CH0-0674
CH0-4197
CH0-1170
CH0-4201
CH0-4202
CH0-1643
CH0-1644
Mfr. No.
32908
32918
79447
79445
79317
79461
79448
79446
79383
79456
79449
79368
79460
79376
79377
79462
79463
Price
All other Hamilton Columns and Bulk Packings not listed are available.
176
Phenomenex
HyperClone
If HyperClone analytical columns do not

compared to Hypersil columns of the
same phase, particle size and dimensions,
Guaranteed Replacement to Hypersil

Highly reproducible
Long column life
Mimics performance of Thermo Hypersil-Keystone HYPERSIL
Economically priced
Phenomenex HyperClone columns have been developed to provide chromatographic
behavior that mimics that of Thermo HYPERSIL columns. For comparative applications,
please contact your local Phenomenex representative.
Comparisons of physical and chemical characteristics of HyperClone and Hypersil
are listed below. As you can see, HyperClone and Hypersil compare very well for
important specifications such as particle size, pore size and carbon load.
HyperClone
vs.
SEM of Base Silica
Hypersil
HyperClone (BDS silica)
3, 5
130
155
0.6
Hypersil (BDS silica)
Particle Size (m)

Pore Size ()
Surface Area (m2/g)
Pore Volume (mL/g)
HyperClone (regular silica)
Hypersil (regular silica)
3, 5
120
170
0.6
Particle Size (m)

Pore Size ()
Surface Area (m2/g)
Pore Volume (mL/g)
HyperClone
HyperClone
7
11
6.5
10
4
3, 5
130
170
0.6
HPLC
3, 5
120
155
0.6
SEM of Base Silica
Parameter
Hypersil
Carbon Load % Comparison

BDS C8
BDS C18
MOS (C8)
ODS (C18)
CN (CPS)
vs.
HyperClone
7
11
6.5
10
4
Hypersil
Size
HyperClone Reproducibility
Silica Reproducibility
11
Batch Batch
Number Number
Phenomenex
5410-04
15
5410-03
10
Batch Number
5410-02
Batch Number
% Carbon
8
5410-01
9
8.5
5411-05
PD
5411-04
PV x100
50
5411-03
100
10
9.5
5411-02
SA
5411-01
Value
150
% Carbon
10.5
200
Carbon Load Data
12
11.5
All Hypersil information obtained from (then) Thermo Electron Corporaton 2006-2007 catalog.
177
HyperClone
If HyperClone analytical columns do not

compared to Hypersil columns of the
same phase, particle size and dimensions,
VS.
HyperClone
14635
HYPERSIL
Conditions for Both Columns
14636
1
1
5 m BDS C18
400
Non-Polar Basic Compounds

Dimensions:
Mobile Phase:
Flow Rate:
Detection:
Sample:
5 m BDS C18
400
mAU
200
App ID 14635
App ID 14636
200
mAU
150 x 4.6 mm
Methanol/50 mM KH2PO4, pH 3.5 (60:40)
1.0 mL/min
UV @ 254 nm
1. Uracil
2. Pyridine
3. Methylaniline
4. Dimethylaniline
5. Dichloronitroaniline
6. Toluene
0
0
10
14 min
12
10
12
14 min
3 m Minibore and Analytical Columns (mm)

Phases
50 x 2.0
150 x 2.0
ODS (C18)
00F-4356-B0
CN (CPS)
150 x 3.2
100 x 4.6
125 x 4.0
150 x 4.6
00F-4356-R0
00D-4356-E0
00D-4421-E0
00E-4356-D0
00F-4356-E0
00F-4421-E0
BDS C8
BDS C18
00F-4419-R0
00D-4417-E0
00D-4419-E0
00E-4419-D0
00F-4417-E0
00F-4419-E0
00B-4417-B0
00F-4417-B0
00F-4419-B0

4 x 2.0*
4 x 3.0*
/10pk
/10pk
AJ0-4286
AJ0-4287
AJ0-4304
AJ0-4305
/10pk
/10pk
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
for ID: 2.0-3.0 mm
3.2-8.0 mm
HyperClone
HPLC
5 m Minibore and Analytical Columns (mm)

Phases
150 x 2.0
150 x 3.2
Silica
MOS (C8)
ODS (C18) 00F-4361-B0

CN (CPS)
PAH
00F-4361-R0
BDS C8
BDS C18
00F-4420-R0
00F-4420-B0
250 x 3.2
125 x 4.0
250 x 4.0
100 x 4.6
150 x 4.6
250 x 4.6
00G-4361-R0
00E-4359-D0
00E-4361-D0
00G-4361-D0
00D-4361-E0
00D-4427-E0
00F-4358-E0
00F-4359-E0
00F-4361-E0
00F-4422-E0
00G-4358-E0
00G-4359-E0
00G-4361-E0
00G-4422-E0
00G-4427-E0
00E-4420-D0
00G-4420-D0
00D-4420-E0
00F-4418-E0
00F-4420-E0
00G-4418-E0
00G-4420-E0

4 x 2.0*
4 x 3.0*
/10pk
/10pk
AJ0-4347
AJ0-4348
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
AJ0-4304
AJ0-4305
/10pk
/10pk
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
for ID: 2.0-3.0 mm
5 m SemiPrep and Preparative Columns (mm)

Phases
250 x 10
250 x 21.2
ODS (C18)
00G-4361-N0
BDS C18
00G-4420-N0
3.2-8.0 mm

10 x 10
15 x 21.2**
/3pk
/ea
AJ0-7221
AJ0-7839
/3pk
AJ0-7221
00G-4361-P0
for ID: 9-16 mm
18-29 mm

Other dimensions available upon request.
For SecurityGuard Cartridge Holders and Cartridges. see p. 235.
HyperClone is a trademark of Phenomenex.Inc.

Hypersil is a registered trademark of Thermo Hypersil-Keystone.
Phenomenex is not associated with Thermo Fisher Scientific or Thermo Hypersil-Keystone.
Comparative chromatograms may not be representative of all applications.
178
Phenomenex
Hypersil * and Hypersil BDS
Hypersil
5 m Columns (mm)
Phases
250 x 2.0
Silica
C8 (MOS)
C18 (ODS)
CN (CPS)
NH2 (APS-1)
SecurityGuard Analytical Cartridges require universal holder Part No.: KJO-4282
00G-0152-B0
150 x 3.2
150 x 4.6
250 x 4.6
00F-0152-R0
00F-0151-E0
00F-0152-E0
00F-0153-E0
00F-0154-E0
00G-0149-E0
00G-0151-E0
00G-0152-E0
00G-0153-E0
00G-0154-E0
SecurityGuardCartridges (mm)
4 x 2.0
4 x 3.0
/10pk
/10pk
AJ0-4347
AJ0-4348
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
AJ0-4304
AJ0-4305
AJ0-4301
AJ0-4302
for ID: 2.0-3.0 mm
Use HyperClone
Columns as a Guaranteed
Alternative to Hypersil
See p. 177
3.2-8.0 mm
Hypersil BDS
3 m Columns (mm)
Phase
50 x 2.0
C8-BDS
00B-4017-B0
For SecurityGuard Cartridge Holders and

Cartridges, see p. 235.
4 x 2.0
4 x 3.0
/10pk
/10pk
AJ0-4289
AJ0-4290
150 x 4.6
00F-4017-E0
for ID: 2.0-3.0 mm
*Hypersil is a registered trademark of Thermo Hypersil-Keystone.

Phenomenex is not affiliated with Thermo Fisher Scientific or Thermo Hypersil-Keystone.
3.2-8.0 mm
5 m Columns (mm)
Phase
50 x 2.0
C18 - BDS
00B-4022-B0
150 x 3.2
50 x 4.6
100 x 4.6
150 x 4.6
00F-4022-R0
00B-4022-E0
00D-4022-E0
00F-4022-E0
4 x 2.0
4 x 3.0
/10pk
/10pk
AJ0-4286
AJ0-4287
250 x 4.6
00G-4022-E0
for ID: 2.0-3.0 mm
Hypercarb
3.2-8.0 mm
* Hypercarb is a trademark of Thermo Scientific, part of Thermo Fisher Scientific.
HPLC
For Availability and Ordering Information please contact your Phenomenex Technical Consultant. (See p. 317)
Inertsil
Many popular sizes in stock
Guaranteed replacement to Inertsil, see Prodigy p. 226
3 m Columns (mm)
Phase 100
100 x 4.6
150 x 4.6
ODS-3
CH0-5206
CH0-5207
*Inertsil is a registered trademark of GL Sciences Inc., Japan.

Phenomenex is not affiliated with GL Sciences Inc., Japan.
Inertsil prices shown are set by Phenomenex.
5 m Columns (mm)
Phases
Phase 150
ODS-2
C8
Ph (Phenyl)
Phase 100
ODS-3
150 x 3.0
250 x 3.0
100 x 4.6
150 x 4.6
250 x 4.6
00F-3125-Y0
00F-3137-Y0
00G-3125-Y0
00D-3125-E0
00F-3125-E0
00F-3136-E0
00F-3137-E0
00G-3125-E0
00G-3136-E0
00G-3137-E0
CH0-5170
CH0-5171
hypersil / hypersil bds / hypercarb / IB-Sil / inerTsil
IB-Sil

4 x 2.0
4 x 3.0
/10pk
/10pk
AJ0-4286
AJ0-4287
AJ0-4289
AJ0-4290
AJ0-4350
AJ0-4351
/10pk
/10pk
AJ0-4286
AJ0-4287
for ID: 2.0-3.0 mm
3.2-8.0 mm
(1) Part Nos. beginning with CH0- are packed by GL Sciences Inc., Japan and resold by Phenomenex.
(2) Part Nos. beginning with 00_- are packed by Phenomenex using GL Sciences manufactured bulk media.
(3) Inertsil columns above carry the Phenomenex warranty.
Phenomenex
179
Kinetex
If Kinetex core-shell columns do not

the same phase, return the column with
the comparative data within 45 days for
a FULL REFUND.
Ultra-High Performance on Any LC System

Achieve sub-2 m performance within HPLC backpressure limitations

Substitute 3 m and 5 m columns for 2-3x higher efficiency
Obtain higher throughput without sacrificing resolution
Easy method transfer across LC system platforms
Reduce solvent consumption with faster analysis
Reach lower levels of detection and quantitation
Kinetex 1.7 m
Core-Shell Particle
Kinetex 2.6 m
Core-Shell Particle
Reduced diffusion path

maximizes efficiency
Reduced diffusion path

maximizes efficiency
Increased efficiencies
compared to traditional
fully porous sub-2 m
columns. Typical operating
backpressures > 400 bar
Ultra-high performance
on any system with
Kinetex 2.6 m columns
1.25 m Solid Core
1.9 m Solid Core
2010 R&D 100

Award Recipient
0.35 m Porous Shell
0.23 m Porous Shell

2.6 m
1.7 m
For more information see p. 186
HPLC
Packing
Material
kinetex
Kinetex XB-C18
Kinetex C18
Kinetex C8
Kinetex PFP
Kinetex HILIC
Kinetex XB-C18
Kinetex C18
Kinetex C8
Kinetex PFP
Kinetex HILIC
Total
Particle
Size
(m)
Porous
Shell
(m)
Solid
Core
(m)
Pore
Size
()
Effective
Surface
Area
(m2/g)
Effective
Carbon
Load
%
pH
Stability
2.6
2.6
2.6
2.6
2.6
2.6
1.7
1.7
1.7
1.7
1.7
1.7
0.35
0.35
0.35
0.35
0.35
0.35
0.23
0.23
0.23
0.23
0.23
0.23
1.9
1.9
1.9
1.9
1.9
1.9
1.25
1.25
1.25
1.25
1.25
1.25
100
100
100
100
100
100
100
100
100
100
100
100
200
200
200
200
200
200
200
200
200
200
200
200
10
12
8
9
0
11
10
12
8
9
0
11
1.5 - 8.5**
1.5 - 8.5**
1.5 - 8.5**
1.5 - 8.5**
2.0 - 7.5
1.5 - 8.5**
1.5 - 8.5**
1.5 - 8.5**
1.5 - 8.5**
1.5 - 8.5**
2.0 - 7.5
1.5 - 8.5**
Pressure
Stability
1000/600
bar
1000 bar
**Columns are pH stable from 1.5-10 under isocratic conditions. Columns are pH stable 1.5-8.5 under gradient conditions.
2.1 mm ID Kinetex columns are pressure stable up to 1000 bar.
Innovation in Particle Technology

The Kinetex core-shell particle is not fully porous. Using sol-gel processing techniques that incorporate nano structuring technology, a durable, homogenous porous shell is grown on a solid silica core.
This highly optimized process combined with uniform particle size distribution produces a column
that generates extremely high plate counts. When using Kinetex 2.6 m, less column backpressure is
generated, allowing it to be used on any LC system.
SEM of Kinetex Core-Shell Particles
Cross Section of Kinetex Core-Shell Particle
When using Kinetex 1.7 m, increased performance can be achieved, however higher pressure-capable instrumentation is required.
180
Phenomenex
Kinetex
Optimized for Ultra-High

Performance
Faster Mass Transfer
Kinetex Core-Shell
Since the Kinetex particle is not fully porous, analytes spend less
time diffusing into and out of the pores as they travel through the
column. This shorter diffusion path allows for faster mass transfer.
The result is less band broadening for higher peak efficiency comparable to or better than sub-2m fully porous particles.
Band Broadening
High Efficiency over Extended Range of Flow Rates

30
Fully Porous
25
Double the efficiency

of 3 m at high speed
HETP (m)
20
15
Band Broadening
Sub-2 m exceeds
conventional pressure here
10
3 m
Linear Velocity (mm/s)
10
18755
HPLC |
Kinetex 2.6 m Core-Shell

Sub-2 m Fully Porous
3 m Fully Porous
18756
kinetex
Kinetex particles are nearly monodispersed. This extremely narrow particle size distribution results in ultra-high
column efficiency and excellent reproducibility.
Uniform Particle Size Distribution
Batch-to-Batch Reproducibility Overlay

3
Batch 68 Rs (2,3) 2.00
Batch 73 Rs (2,3) 2.13
Batch 75 Rs (2,3) 2.06
Kinetex 2.6 m Core-Shell

Traditional Fully Porous 1.7 m
Traditional Fully Porous 3 m
2000
4
5
1
1000
Batch 68
Batch 73
Batch 75
3
Particle Diameter (m)
min
App ID 18755, 18756, 18757
Particle Count
3000
Conditions same for all batches:

Column: Kinetex 2.6 m C18
Part No.: 00B-4462-E0
Mobile Phase: Water / Acetonitrile (65:35)
Sample: 1. Uracil
2. Hydroxycortisone
3. Cortisone
4. Cortisone acetate
5. 17-Hydroxyprogesterone
Phenomenex
181
Kinetex
Achieve Sub-2 m Performance within HPLC Backpressure Limitations

With the efficiency of a sub-2 m column and typical operating backpressure less than 400 bar, you can achieve the promise of ultra-high
performance
on any LC system.
18165
> 400 bar!
Method cannot transfer to
a non-UHPLC instrument
mV
400
18166
Traditional 1.7 m
mV
400
< 300 bar!

Easier method transfer
to ANY system
Kinetex 2.6 m
N = 272,080 p/m
3
0
0
N = 267,720 p/m
200
App ID 18165
4
2
App ID 18166
200
min
2 min
kinetex
HPLC

Traditional 1.7 m C18
Mobile Phase: Acetonitrile / Water (50:50)
Temperature: 25 C
Instrument: Waters ACQUITY UPLC
Sample: 0.5 L test mixture
1. Acetophenone 3. Toluene
2. Benzene
4. Naphthalene
Unparalleled Levels of Ultra-High Performance

For users of higher pressure capable instruments who want the highest level of efficiency, we introduce the Kinetex 1.7 m columnthe first
sub-2 m core-shell particle available on the market.
18167
18165
Traditional 1.7 m
Kinetex 1.7 m
mV
mV
400
400
N = 272,080 p/m
N = 318,680 p/m
1
1
200
App ID 18167
App ID 18165
200
min
min

Temperature: 25 C
Sample: 1. Acetophenone 3. Toluene
2. Benzene
4. Naphthalene
Kinetex 2.6 m columns, 2.1 mm ID, are pressure rated to 1000 bar use on both HPLC and UHPLC instrumentation.
Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation. Phenomenex is not affiliated with Waters Corporation.
182
Phenomenex
Kinetex
Substitute 3 m and 5 m Columns for 2-3x Higher Efficiency

Replace traditional 3 m or 5 m analytical columns with Kinetex 2.6 m core-shell columns for immediate performance improvements in
efficiency,18628
speed, resolution, and sensitivity. Optimize methods for ultra-high performance and transfer them to any system.
Traditional 3 m
18539
Kinetex 2.6 m
mV
mV
240
240
210
N = 166,502 p/m
210
150
150
90
2
1
30
90
App ID 18628
60
120
App ID 18539
120
N = 295,343 p/m
180
180
60
1
30
0
0
min
min

Traditional 3 m C18
Temperature: 25 C
Backpressure: 380 bar (Kinetex)
250 bar (Traditional 3 m)
Instrument: Agilent 1200SL
Sample: 1. Uracil
3. Toluene
2. Acetophenone 4. Naphthalene
For the ultimate sample throughput demands, Kinetex columns provide the efficiency
needed to significantly reduce run times. In this
18276
separation
18280 of 13 ketones, a 20-fold increase in productivity is accomplished while still maintaining resolution.
> 60 min
Rs10,11 = 3.11
4
1
11
11
7
12
20
Column: Traditional 5 m C18

B: Acetonitrile
Gradient: Time (min)
%B

0
5
4.78
5
51.52
95
Temperature: 45 C
Sample: 1. Acetone
2. 2-Butanone
3. 2-Pentanone
4. Acetophenone
5. 2-Heptanone
6. Butyrophenone
7. 2-Nonanone
40
Time (min)
66
66.01
86.38
%B
95
5
5
8. Hexanophenone
9. Octanophenone
10. 2-Tridecanone
11. Decanophenone
12. 2-Pentadecanone
13. 2-Hexadecanone
60
13
min
App ID 18280
10
Kinetex 2.6 m
10
Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Temperature:
Detection:
Backpressure:
Sample:
Kinetex 2.6 m C18

50 x 4.6 mm
00B-4462-E0
A: Water
B: Acetonitrile
Time (min)
%B

0
5
0.23
5
2.19
95
3.4 mL/min
45 C
UV @ 258 nm
350 bar
1. Acetone
2. 2-Butanone
3. 2-Pentanone
4. Acetophenone
5. 2-Heptanone
6. Butyrophenone
7. 2-Nonanone
Time (min)
2.75
2.76
3.61
12
13
App ID 18276
< 3 min
Resolution maintained
Rs10,11 = 3.12
kinetex
Traditional 5 m
HPLC |
Obtain Higher Throughput without Sacrificing Resolution
min
%B
95
5
5
8. Hexanophenone
9. Octanophenone
10. 2-Tridecanone
11. Decanophenone
12. 2-Pentadecanone
13. 2-Hexadecanone
Phenomenex
183
Kinetex
Easy Method Transfer Across LC System Platforms

UHPLC methods developed with fully porous sub-2 m columns often generate backpressure higher than HPLC system limitations. With Kinetex 2.6 m
core-shell technology, you are no longer restricted from developing high performance LC methods on any system and transferring them anywhere. In these
examples different internal diameters of Kinetex columns are used on various
systems to illustrate the versatility of Kinetex core-shell technology. Please note
the flow rates are scaled to maintain the same linear velocity.
Kinetex 3.0 mm ID
on Shimadzu Prominence UFLCxr
Kinetex 4.6 mm ID
on Agilent 1100
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:
Kinetex 2.6 m C18

50 x 4.6 mm
00B-4462-E0
Acetonitrile / Water (50:50)
2.35 mL/min
Ambient
UV @ 254 nm
1. Uracil
2. Acetophenone
3. Toluene
4. Naphthalene
Kinetex 2.6 m C18

50 x 3.0 mm
00B-4462-Y0
1.0 mL/min
Ambient
UV @ 254 nm
1. Uracil
2. Acetophenone
3. Toluene
18567
4. Naphthalene
4
4
App ID 18565
HPLC
2 min

Part No.: 00B-4462-AN
18568
Sample:
1. Uracil
2. Acetophenone
3. Toluene
4. Naphthalene
Kinetex 2.6 m C18

50 x 2.1 mm
00B-4462-AN
0.49 mL/min
Ambient
UV @ 254 nm
1. Uracil
2. Acetophenone
3. Toluene
4. Naphthalene
2
4
App ID 18566
2 min
Kinetex 2.1 mm ID
on Waters ACQUITY UPLC
Kinetex 2.1 mm ID
on Agilent 1200SL
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:
2 min
App ID 18568
kinetex
App ID 18567
2 min
Agilent is a registered trademark of Agilent Technologies, Inc. Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation. Shimadzu and Prominence are registered trademarks, and UFLC is a trademark
of Shimadzu Corporation. Phenomenex is not affiliated with Agilent Technologies, Shimadzu Corp., or Waters Corp. Comparative separations may not be representative of all applications.
184
Phenomenex
Kinetex
Improve Performance, Save Solvent

When chromatographic column performance improves you can not only decrease your analysis time but also decrease your overall solvent
consumption without compromising your separations. Use Kinetex core-shell technology to dramatically decrease the solvent consumption
in your laboratory and increase sample throughput.
18243
Example Method Consumption
Column: Traditional 5 m C18

110 mL
solvent per run!
6
2
5
100
120 min
20
40
60
80
Less Solvent Consumption with Kinetex Column
Kinetex 2.6 m C18

50 x 2.1 mm
00B-4462-AN
0.6 mL/min
18245
< 4 mL
solvent per run!
5
8
10
min
6. Amoxapine
7. Doxepin
8. Nortriptyline
9. Amitriptyline
10. Clomipramine
kinetex
Sample:
1. Tianeptine
2. Desmethyldoxepin
3. Protriptyline
4. Desipramine
5. Imipramine
HPLC |

Mobile Phase: A: 20 mM Potassium phosphate pH 7
B: Methanol / Acetonitrile (50:50)
A/B (48:52)
Temperature: 40 C
App ID 18245
Column:
Dimensions:
Part No.:
Flow Rate:
10
App ID 18243
Reach Lower Levels of Detection and Quantitation

The combination of the small particle size, narrow particle size distribution, and the significantly shorter diffusion path results in much higher
column efficiencies and increased chromatographic resolution. The increased efficiencies provide an immediate benefit on sensitivity since higher
chromatographic efficiencies translate into significantly narrower and taller peaks, making it easier to detect low level impurities.
ZORBAX 3.5 m SB-C18
18262
mAU
Kinetex 2.6 m C18
18263
mAU
0.8
0.8
0.6
0.6
0.4
0.0
0.2
0.0
6 min

B: Acetonitrile
Gradient: (95:5) A/B for 1.16 min, then to (5:95) A/B
Temperature: 45 C
Instrument: Agilent 1200
App ID 18262
App ID 18263
0.4
0.2
Sample:
1. Pyridine
2. Acetaminophen
3. Pindolol
4. Quinine
5. Acebutolol
6. Chlorpheniramine
7. Triprolidine
8. Prednisolone
6 min
9. Nortriptyline
10. 4-Chlorobenzoic acid
11. 5-Methyl-2-hydroxy benzaldehyde
12. 4-Chlorocinnamic acid
13. Diazepam
14. Diflunisal
15. Niflumic acid
16. Hexanophenone
Zorbax is a registered trademark of Agilent Technologies. Comparative separations may not be representative of all applications.
Phenomenex is not affiliated with Agilent Technologies.
Phenomenex
185
Kinetex
Complementary and Orthogonal Selectivities

To provide alternative and orthogonal selectivity phases, Kinetex columns are available in 6 selectivities: XB-C18, C18, C8, PFP
(Pentafluorophenyl), Phenyl-Hexyl, and HILIC (Hydrophilic Interaction Liquid Chromatography), for resolution of a wide range of compounds
from polar to hydrophobic, aromatic, and isomers.
Kinetex XB-C18
TMS
Si
TMS
HPLC
|
TMS
TMS
TMS
TMS
XBC18
kinetex
Kinetex C8
Kinetex C18
C18
C8
This unique C18 phase yields

increased hydrogen bonding with
hydrophobic selectivity, resulting in
improved peak shape for
basic compounds and increased
retention of acidic compounds
Balanced C18 phase that

provides the highest degree of
hydrophobic selectivity relative
to the other Kinetex phases
Moderate hydrophobic and steric

selectivity is offered, bringing ultrahigh performance to USP L7 and
other octyl silane methods
pH Range:
USP Classification:
Particle Sizes:
Effective Carbon Load:
pH Range:
USP Classification:
Particle Sizes:
1.5 8.5*
L1
2.6 m & 1.7 m
10 %
Kinetex PFP
1.5 8.5*
L1
2.6 m & 1.7 m
12 %
pH Range:
USP Classification:
Particle Sizes:
Kinetex HILIC
ACN H20 ACN
ACN
ACN
TMS
ACN
TMS
H20
Pentafluorophenyl phase offers a

high degree of steric interactions for improved separation of structural isomers,
and the electronegative fluorine groups
can offer increased retention of polar
basic compounds
pH Range:
USP Classification:
Particle Sizes:
1.5 8.5*
L43
2.6 m & 1.7 m
9%
PhenylHexyl
Aromatic and moderate
hydrophobic selectivity result
in the great retention and
separation of aromatic
hydrocarbons
pH Range:
USP Classification:
Particle Sizes:
1.5 8.5*
L11
2.6 m & 1.7 m
11 %
H20
H20
O
Si
ACN H 0 ACN
2
OH
H20
H20
Si
ACN
ACN
H20
HO
Si
PFP
ACN
H20
HO
HO
ACN
ACN
ACN
HO
H20
TMS
ACN
ACN
ACN H 0
2
TMS
1.5 8.5*
L7
2.6 m & 1.7 m
8%
H20
OH
HILIC
Used under HILIC running conditions, this phase provides the
highest polar selectivity for retention and separation of hydrophilic
compounds
pH Range:
USP Classification:
Particle Sizes:
Carbon Load:
2.0 7.5
L3
2.6 m & 1.7 m
*Columns are pH stable from 1.5-10 under isocratic conditions. Columns are pH stable 1.5-8.5 under gradient conditions.
186
Phenomenex
Kinetex
Steroids
Veterinary Drugs
19487
C8
3
2
App ID 19465
XB-C18
6
4
0
19488
min
App ID 19487
19465
min
19466
C18
C18
5
min
18605
Norepinephrine
and Epinephrine
18564
Explosives
18604
C18
PFP
4
5
min
18563
min
C18
4
min

Column: Kinetex 2.6 m HILIC
Kinetex 2.6 m C18
Mobile Phase Acetonitrile / 100 mM
(HILIC): Ammonium formate
pH 3.2 (92:8)
Mobile Phase (C18): 5 mM Ammonium
formate pH 3.2 /
Methanol (97:3)
Temperature: 30 C
Sample: 1. Norepinephrine
2. Epinephrine
App ID 18563
App ID 18604
7
5 6
1
Time (min)
6.01

8
min
%B
20
20
7. Estrone
9. Deoxycorticosterone acetate
Benzodiazepines
App ID 18605
3

Column: Kinetex 2.6 m PFP
Kinetex 2.6 m C18
Dimensions: 100 x 4.6mm
B: Methanol
Gradient: (45:55) A/B to (35:65) A/B
over 6 min
Temperature: 22 C
Sample: 1. HMX
2. RDX
3. 1,3,5-Trinitrobenzene
4. 1,3-Dinitrobenzene
5. Nitrobenzene
6. 2,4,6-Trinitrotoluene
7. 2,4-Dinitrotoluene
min
Phenyl-Hexyl
19904
2.0e6
1.8e6
1.6e6
1.4e6
1.2e6
1.0e6
8.0e5
6.0e5
4.0e5
2.0e5
0.0
19905
1.8e6
1.6e6
1.4e6
1.2e6
1.0e6
8.0e5
6.0e5
4.0e5
2.0e5
0.0
5
7
kinetex

Column: Kinetex 2.6 m XB-C18
Kinetex 2.6 m C18
B: Acetonitrile
%B

0
20

6
60
Temperature: 30 C
Sample: 1. Estriol
2. Hydrocortisone
3. Cortisone
4. Estradiol
5. Cortisone-21-acetate
HILIC
HPLC |

Kinetex 2.6 m C18
Mobile Phase: A: 0.1% Phosphoric acid in Water
B: 0.1% Phosporic acid in Acetonitrile
%B
Time (min)
%B

0
30

9
95

4
50
10
30
7.5
70
Temperature: 25 C
Sample: 1. Naproxen
5. Indomethacin
2. Fenoprofen
6. Meclofenamic acid
3. Ibuprofen
7. Matrix impurity
4. Phenylbutazone
8. Matrix impurity
App ID 19904
1
1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0 min
549 618 686 755 823 892 960 102910981166123513031372
PFP
5+6
7
1+2
4
3
App ID 19905
8
7
Intensity, cps
Intensity, cps
6
4,8
App ID 18564
App ID 19466
App ID 19488
1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0 min

Column: Kinetex 2.6 m Phenyl-Hexyl
Kinetex 2.6 m PFP
Mobile Phase: A: 10 mM Ammonium formate
B: Acetonitrile
%B
0
15
4
55
Temperature: 22 C
Detection: Mass Spectrometer (MS)
Sample: 1. a-Hydroxyalprazolam
2. Oxazepam
3. Lorazepam
4. Clonazepam
5. Nordiazepam
6. Temazepam
7. Diazepam
Phenomenex
187
Kinetex
Applications
Food Testing
Melamine and Cyanuric Acid
Azo Dyes
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Backpressure:
Instrument:
Sample :
Column:
Dimensions:
Part No.:
Mobile Phase:
Kinetex 2.6 m HILIC

50 x 2.1 mm
00B-4461-AN
Acetonitrile / 100 mM Ammonium acetate, pH 5.8 (90:10)
1.0 mL/min
25 C
API 3000 MS
190 bar
Waters ACQUITY UPLC MS/MS
1. Cyanuric acid 128-85.0
(quant ion),128.0-42.0 (qualifier ion)
2. Cyanuric acid-13C3 ISTD 131.1-87.0
3. Melamine 127.1-85 (quant ion),
18723 127.1-68 (qualifier ion)
4. Melamine-13C3.15N3 ISTD 133.2-89.1
Kinetex 2.6 m C18

150 x 4.6 mm
00F-4462-E0
A: 0.1% Phosphoric acid in Water
B: 0.1% Phosporic acid in Acetonitrile
Time (min)
%B
Time (min)

0
25
17.01
15
95
20
17
95
1.8 mL/min
50 C
UV @ 215 nm
380 bar
1. Orange II
6. Sudan I
2. Sudan Orange G
7. Sudan II
3. Fast Garnet GBC
8. Sudan III
4. Dimethyl yellow
9. Sudan IV
5. Sudan Red G
Gradient:
Flow Rate:
Temperature:
Detection:
Backpressure:
Sample:
18589
mV
1,2
4.2e4
3,4
%B
25
25
mAU
80
ESI- ESI+
60
3.0e4
2.2e4
HPLC
0.10
20
1.4e4
kinetex
App ID 18589
App ID 18723
40
0.20
0.30
0.40
min
10
12
14
16 min
Environmental
Carbamate Pesticides: EPA Method 531.1
Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Temperature:
Detection:
Sample :
Polyaromatic Hydrocarbons (PAHs): EPA Method 610

Column:
Dimensions:
Part No.:
Mobile Phase:
Kinetex 2.6 m C18

50 x 2.1 mm
00B-4462-AN
A: 0.1 % Phosphoric acid in Water
B: 0.1 % Phosphoric acid in Acetonitrile
(95:5) A/B to (5:95) A/B over 3 min
1.0 mL/min
40 C
UV @ 210 nm
1. Aldicarb sulfoxide
7. Aldicarb
2. Oxamyl
8. Baygon (Propoxur)
3. Aldicarb sulfone
9. Carbofuran
4. Methomyl
10. Carbaryl
5. 3-OH-Carbofuran
11. 1-Napthol
6. Aldicarb sulfone 12. Methiocarb
related impurity
Kinetex 2.6 m C18

100 x 4.6 mm
00D-4462-E0
A: Water
B: Acetonitrile
Gradient: (30:70) A/B to (0:100) A/B over 10 min
Temperature: 30 C
9. Chrysene
2. Acenaphthylene
3. Fluorene
18552 4. Acenaphthene
5. Phenanthrene
13. Benzo[a]pyrene
6. Anthracene
7. Fluoranthene
8. Pyrene
18610
12
1 2
10 11
12 13
App ID 18610
11
min
1 2 34
1
78
15
14
3
App ID 18552
10
16
min
API 3000 is a trademark of AB SCIEX.

Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation. Phenomenex is not affiliated with AB SCIEX or Waters Corporation.
188
Phenomenex
Kinetex
Applications
Clinical Toxicology
25-OH Vitamin D2 and D3 from Serum
Digoxin and Digitoxin in Plasma
Column:
Dimensions:
Part No.:
Mobile Phase:
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample :
Gradient:
Flow Rate:
Temperature:
Detection:
Sample :
19691
19313
25-OH D3-d6 (IS)
6.0e4
Intensity, cps
Intensity cps
5.0e4
4.0e4
25-OH D3
3.0e4
25-OH D2
1.0e4
3.6
3.7
3.8
3.9
4.0
4.1
4.2
4.3
4.4
4.5
4.6 min
2300
2200
2100
2000
1900
1800
1700
1600
1500
1400
1300
1200
1100
1000
900
800
700
600
500
400
300
200
100
0
2.0
2.5
0.5
1.0
1.5
3.0
3.5
4.0
4.5 min
kinetex
Pharmaceutical
Atenonol Impurity Profile
Forced Degradation of Ranitidine
Column:
Dimensions:
Part No.:
Mobile Phase:
Column:
Dimensions:
Part No.:
Mobile Phase:
Kinetex 2.6 m C18

100 x 4.6 mm
00D-4462-E0
12.5 mM Phosphoric acid in Water, pH 3.0 + 2.0 g
Sodium Octanesulfonate + 0.8 g Tetrabutyl Ammonium
Hydrogen Sulfate / Methanol / THF (80:18:2)
Temperature: 22 C
Sample: 1. Impurity B
5. Impurity D and E
6. Impurity F
2. Impurity A
7. Impurity G
3. Impurity J
8. Impurity H
4. Impurity I
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:
18519
mAU
6
mAU
100
4
3
4
0
0
Phenomenex
7
0
10
Degradation
Products
40
60
4
3
80
App ID 18519
mAU
6
15
min
Degradation Products
20
App ID 18600
2 3
Kinetex 2.6 m C18

150 x 4.6 mm
00F-4462-E0
A: 0.1 % Formic acid in Water
5 % to 20 % B in 7 min 20 % to 95 % in 2 min
1.4 mL/min
30 C
UV @ 230 nm (22 C)
Ranitidine 1 mg/mL in Methanol. Heated at 65 C for 4 days.
18600
5
18519
HPLC |
App ID 19313
2.0e4
Kinetex 2.6 m C18

50 x 2.1 mm
00B-4497-AN
A: 10 mM Ammonium acetate
B: 10 mM Ammonium acetate in Methanol
Time (min)
%B
0
50
2.5
100
2.51
50
5
50
0.4 mL/min
30 C
MS @ 350 C
Concentration 0.25 mg/mL each
1. Digoxin
2. Oleandrin (IS)
3. Digitoxin
App ID 19691
Gradient:
Kinetex 2.6 m C18

50 x 4.6 mm
00B-4462-E0
A: 0.05 % Formic acid
B: 5 mM Ammonium acetate + 0.1 % Formic acid in Methanol
Time (min)
%B
0
8
5
8
205
100
290
100
360
8
1.0 mL/min
35 C
MS (ambient)
1. 25-hydroxyvitamin D3 (25-OH D3)
2. 25-hydroxyvitamin D3-d6 (25-OH D3-d6)
3. 25-hydroxyvitamin D2 (25-OH D2)
10
min
189
If Kinetex core-shell columns do not provide at least an

equivalent separation as compared to a competing column
of the same phase, return the column with the comparative
Kinetex
2.6 m Analytical Columns (mm)
Phases
30 x 4.6
XB-C18
C18
C8
PFP
HILIC
Phenyl-Hexyl
00A-4462-E0
00A-4477-E0
00A-4461-E0
2.6 m MidBore Columns (mm)

Phases
30 x 3.0
XB-C18
C18
C8
PFP
HILIC
Phenyl-Hexyl
00A-4496-Y0
00A-4462-Y0
00A-4497-Y0
00A-4477-Y0
00A-4461-Y0
2.6 m Minibore Columns (mm)

Phases
30 x 2.1
kinetex
HPLC
XB-C18
C18
C8
PFP
HILIC
Phenyl-Hexyl
00A-4496-AN
00A-4462-AN
00A-4497-AN
00A-4477-AN
00A-4461-AN

Phases
30 x 3.0
XB-C18
C18
C8
PFP
HILIC
Phenyl-Hexyl
00A-4498-Y0
00A-4499-Y0

Phases
30 x 2.1
XB-C18
C18
C8
PFP
HILIC
Phenyl-Hexyl
00A-4498-AN
00A-4475-AN
00A-4499-AN
00A-4476-AN
00A-4474-AN
50 x 4.6
75 x 4.6
100 x 4.6
150 x 4.6
00B-4496-E0
00B-4462-E0
00B-4497-E0
00B-4477-E0
00B-4461-E0
00B-4495-E0
00C-4496-E0
00C-4462-E0
00C-4497-E0
00C-4477-E0
00C-4461-E0
00D-4496-E0
00D-4462-E0
00D-4497-E0
00D-4477-E0
00D-4461-E0
00D-4495-E0
00F-4496-E0
00F-4462-E0
00F-4497-E0
00F-4477-E0
00F-4461-E0
00F-4495-E0
50 x 3.0
75 x 3.0
100 x 3.0
150 x 3.0
00B-4496-Y0
00B-4462-Y0
00B-4497-Y0
00B-4477-Y0
00C-4496-Y0
00C-4462-Y0
00C-4497-Y0
00C-4477-Y0
00D-4496-Y0
00D-4462-Y0
00D-4497-Y0
00D-4477-Y0
00F-4496-Y0
00F-4462-Y0
00F-4497-Y0
00F-4477-Y0
00F-4461-Y0
100 x 2.1
150 x 2.1
00B-4496-AN
00B-4462-AN
00B-4497-AN
00B-4477-AN
00B-4461-AN
00B-4495-AN
00D-4496-AN
00D-4462-AN
00D-4497-AN
00D-4477-AN
00D-4461-AN
00D-4495-AN
00F-4496-AN
00F-4462-AN
00F-4497-AN
00F-4477-AN
00F-4461-AN
100 x 3.0
00B-4498-Y0
00B-4475-Y0
00B-4499-Y0
00B-4474-Y0
00D-4498-Y0
00D-4475-Y0
00D-4499-Y0
00D-4476-Y0
AJ0-8775
AJ0-8775
AJ0-8777
AJ0-8780
AJ0-8779
AJ0-8781
for 3.0 mm ID
AJ0-8782
AJ0-8782
AJ0-8784
AJ0-8787
AJ0-8786
AJ0-8788
for 2.1 mm ID
SecurityGuard
ULTRA Cartridges
3/pk
AJ0-8775
AJ0-8775
AJ0-8777
AJ0-8780
AJ0-8779
AJ0-8781
for 3.0 mm ID
100 x 2.1
150 x 2.1
00B-4498-AN
00B-4475-AN
00B-4499-AN
00B-4476-AN
00B-4474-AN
00B-4500-AN
00D-4498-AN
00D-4475-AN
00D-4499-AN
00D-4476-AN
00D-4474-AN
00D-4500-AN
00F-4498-AN
00F-4475-AN
00F-4499-AN
00F-4476-AN
00F-4500-AN
SecurityGuard ULTRA Cartridge System

The SecurityGuard ULTRA cartridge system protects ultra-high performance columns, like Kinetex, from damaging contaminants and
microparticulates.
New High Pressure
Rated Format
SecurityGuard ULTRA cartridges require holder, Part No.: AJ0-9000
SecurityGuard
ULTRA Cartridges
3/pk
50 x 2.1
Extend Kinetex column lifetime
AJ0-8768
AJ0-8768
AJ0-8770
AJ0-8773
AJ0-8772
AJ0-8774
for 4.6 mm ID
SecurityGuard
ULTRA Cartridges
3/pk
SecurityGuard
ULTRA Cartridges
3/pk
50 x 2.1
50 x 3.0
SecurityGuard
ULTRA Cartridges
3/pk
AJ0-8782
AJ0-8782
AJ0-8784
AJ0-8787
AJ0-8786
AJ0-8788
for 2.1 mm ID
SecurityGuard ULTRA Cartridge Holders

Part No.
Description
AJ0-9000 SecurityGuard ULTRA Cartridge Holder
Unit
ea
Price
Simple to use
For a convenient, disposable in-line filter that fits
virtually all UHPLC / HPLC columns 1.0 to 4.6 mm ID,
use KrudKatcher Ultra, found on p. 17.

Fits virtually all manufacturers columns
UHPLC / HPLC Sure-Lok High Pressure PEEK Male Nut Fittings

Part No.
Description
AQ0-8503 Sure-Lok High Pressure PEEK 1-Pc Nut 10-32,
for 1/16 in. Tubing, 12,000 psi (827 bar)
AQ0-8530 Sure-Lok Fitting Tightening Tool, Aluminum
Unit
10/pk
ea
For Ultra-High Performance Stainless

Steel Nut and Ferrule Set, see p. 361
Price
AQ0-8503
AQ0-8530
For Column Heaters, see p. 357
See p. 361 for more information.
190
Phenomenex
Kromasil
High efficiency 3.5 m silica particles available in

C8 and C18 phases identical bonding to 5 m
Kromasil is a spherical, totally porous silica manufactured by Eka

Chemicals. Kromasil is available for analytical, preparative, and process scale chromatography.
Very high loading capacity
Kromasil can withstand pH levels up to 9.0 and has a narrow pore

size distribution.
Excellent chemical and mechanical stability

Popular for scale-up applications
Packing Material
Kromasil C4
Kromasil C8
Kromasil C18
Particle
Shape/Size
(m)
Pore
Size
()
Pore
Volume
(mL/g)
Surface
Area
(m2/g)
Carbon
Load
%
Calculated Bonded
Phase Coverage
(mole/m2)
End
Capping
Spher. 3.5, 5, 7, 10, 13, 16

Spher. 3.5, 5, 7, 10, 13, 16
Spher. 3.5, 5, 7, 10, 13, 16
100
100
100
0.9
0.9
0.9
340
340
340
8
12
19
3.8
3.6
3.1
Yes
Yes
Yes
3.5 m Columns (mm)
Phases
150 x 2.0
C8
C18
00F-4282-B0
50 x 4.6
100 x 4.6
150 x 4.6
00B-4283-E0
00D-4283-E0
00F-4283-E0

4 x 2.0*
4 x 3.0*
/10/pk
/10pk
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
for ID: 2.0-3.0 mm
5 m Columns (mm)
Phases
250 x 2.0
00G-3033-B0
250 x 4.6
00F-3104-E0
00F-3091-E0
00F-3033-E0
00G-3104-E0
00G-3091-E0
00G-3033-E0

4 x 2.0*
4 x 3.0*
/10pk
/10pk
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
for ID: 2.0-3.0 mm
3.2-8.0 mm
Kromasil
C4
C8
C18
150 x 4.6
3.2-8.0 mm
C18
00G-3109-E0
SecurityGuard
Cartridges (mm)
4 x 3.0*
/10pk
AJ0-4287
HPLC
10 m Columns (mm)
Phases
250 x 4.6
for ID: 3.2-8.0 mm

* Kromasil is a registered trademark of Eka Chemicals.
Phenomenex is not affiliated with Eka Chemicals.
Phenomenex
191
LiChrosorb *
Quality-packed columns by Phenomenex

LiChrosorb is a well-established, rugged, irregular silica material,
with high surface area (60 , 500 m2/g). It is very reproducible and
used by chromatographers worldwide.
5 m Columns (mm)
Phases
125 x 4.0
Inquire
00E-0233-D0
RP-8
NH2
250 x 4.0
Inquire
00G-0233-D0
00G-2120-D0
SecurityGuard
250 x 4.6
4 x 3.0
Inquire
/10pk
00G-0233-E0
AJ0-4290
00G-2120-E0
AJ0-4302
150 x 4.6
Inquire
00F-0233-E0
00F-2120-E0
for ID: 3.2-8.0 mm
10 m Columns (mm)
Phases
SecurityGuard
250 x 4.6
4 x 3.0
Inquire
/10pk
00G-0237-E0
AJ0-4290
00G-0238-E0
AJ0-4287
250 x 4.0
Inquire
00G-0237-D0
00G-0238-D0
RP-8
RP-18
for ID: 3.2-8.0 mm
LiChrospher *
HPLC |
Lichrosorb / LiChrospher
Quality-packed by Phenomenex
LiChrospher (the 4 m material is also known as Superspher in
Europe) is a spherical alternative to the well-established LiChrosorb
irregular material. It offers higher efficiencies than the LiChrosorb
material along with the other benefits associated with spherical
silicas.
4 m (Superspher) Columns (mm)
Phases
125 x 2.0
Inquire
RP-8
00E-3043-B0
RP-18
75 x 4.0
Inquire
00C-3043-D0
125 x 4.0
Inquire
00E-3042-D0
00E-3043-D0
250 x 4.0
Inquire
00G-3042-D0
00G-3043-D0

4 x 2.0
4 x 3.0
/10pk
/10pk
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
for ID: 2.0-3.0 mm
5 m Columns (mm)
Phases
Silica 60
RP-8
RP-18
RP-8 endcapped
RP-18 endcapped
CN
RP-Select B
125 x 4.0
Inquire
00E-3047-D0
00E-3049-D0
00E-3050-D0
00E-3051-D0
00E-3052-D0
00E-3053-D0
00E-3156-D0
250 x 4.0
Inquire
00G-3047-D0
00G-3049-D0
00G-3050-D0
00G-3051-D0
00G-3052-D0
00G-3053-D0
00G-3156-D0
150 x 4.6
Inquire
00F-3049-E0
00F-3050-E0
00F-3052-E0
250 x 4.6
Inquire
00G-3047-E0
00G-3049-E0
00G-3050-E0
00G-3051-E0
00G-3052-E0
00G-3053-E0
00G-3156-E0
3.2-8.0 mm

4 x 2.0
4 x 3.0
/10pk
/10pk
AJ0-4347
AJ0-4348
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
AJ0-4304
AJ0-4305
for ID: 2.0-3.0 mm
3.2-8.0 mm
Other column dimensions available upon request.

Other LiChrospher phases available from Phenomenex: NH2, Diol. See p. 208
* LiChrosorb and LiChrospher are registered trademarks of Merck KGaA, Darmstadt, Germany.
192
Phenomenex
Luna
If Luna analytical columns do not provide

at least an equivalent separation as
compared to a competing column of
FULL REFUND.
One of the Worlds Leading

HPLC Columns
Develop or Improve your HPLC Method
Exceptionally rugged USP phases
Easy method scalability with 3, 5, 10, 10-PREP and 15 m media
Extensive batch traceability and reproducibility data supplied with every column
Particle
Shape/Size
(m)
Pore
Size
()
Surface
Area
(m2/g)
Spher. 3, 5,10, 10-PREP, 15

Spher. 5,10
Spher. 5,10
Spher. 3, 5,10, 10-PREP, 15
Spher. 5,10
Spher. 2.5, 3, 5,10, 10-PREP, 15
Spher. 3, 5,10, 10-PREP, 15
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 5,10
Spher. 3, 5
Spher. 3, 5
100
100
100
100
100
100
100
100
100
100
200
100
400
440
440
400
440
400
400
400
400
400
200
400
Packing Material
Luna Silica(2)
Luna C5
Luna C8
Luna C8(2)
Luna C18
Luna C18(2)
Luna Phenyl-Hexyl
Luna CN
Luna NH2
Luna SCX
Luna HILIC
Luna PFP(2)
Carbon
Load
%
Calculated Bonded
Phase Coverage
(mole/m2)
12.5
7.85
14.75
5.50
13.5
5.50
19
3.00
17.5
3.00
17.5
4.00
7.0
3.80
9.5
5.80
Binding Capacity: 0.15 meq/g
5.7
4.30
11.5
2.20
End
Capping
pH
Stability
No
Yes
Yes
Yes
Yes
Yes
Yes
Yes
No
No
No
Yes
2.0 - 7.5
1.5 - 9.0*
1.5 - 9.0*
1.5 - 9.0*
1.5 - 9.0*
1.5 - 9.0*
1.5 - 9.0*
1.5 - 7.0
1.5 - 11
2.0 - 7.0
1.5 - 8.0
1.5 - 8.0
SFC
APPROVED
* pH range is 1.5-10 under isocratic conditions. pH range is 1.5-9 under gradient conditions.
USP Phases for Virtually Every Application

USP Column
Classification
Common Applications
L1
2.5, 3, 5, 10, 10-PREP, 15 m

C18 phase. Excellent efficiency, peak shape and resolution. Slightly lower
carbon load than original Luna C18.
Acetaminophen, Aspirin, Caffeine, Albuterol, Amitriptyline Hydrochloride, Amoxicillin,

Atenolol, Cephalexin, Cephradine capsules, Chloramphenicol, Cortisone Acetate,
Dextromethorphan, Diphenhydramine, Pseudoephedrine, Dopamine, Estradiol,
Guaifenesin, Ibuprofen, Sterile Imipenem, Imipramine, Lidocaine, Lorazepam,
Minoxidil, Naproxen, Phenylephrine Hydrochloride, Phenylpropanolamine, Prednisone
oral solution, Procainamide, Propoxyphene, Reserpine
L3
L7
Silica(2)
3, 5, 10 m
Ultra-pure silica with high column bed stability enhanced by particle shape
uniformity.
Alprazolam, Hydrocodone bitartrate, Hydrocortisone, Fat Soluble Vitamins, Phthalates,

Fatty Acids, Lutein, Lycopene, Estradiol
C8(2)
3, 5, 10, 10-PREP, 15 m
C8 phase for excellent efficiency, peak shape and resolution. Significantly
improved performance over traditional C8 phases due to high surface
coverage.
Doxepine, Doxylamine succinate, Fluoxetine, Glyburide, Ibuprofen Oral Suspension,

Propranolol, Levonorgestrel, Ethinyl estradiol, Melengestrol acetate, Glucosamine
L8
NH2
3, 5, 10 m
Amino phase. Can be used in reversed or normal phase modes.
Stable from pH 1.5 to 11.0 and under 100% aqueous conditions.
High performance silica and bonding techniques produces a rugged, highly
reproducible column.
Simple sugars, Carboplatin, Lactulose concentrate, Levocarnitine tablets
L9
L10
SCX
5, 10 m
A Benzene Sulfonic Acid bonded phase is used to make this Strong Cation
Exchange (SCX) column. Offers great peak shape and resolution.
Cough and cold compounds, Raclopride, Sodium Acetate, Erythromycin
CN
3, 5, 10 m
Cyano phase. Can be used as reversed- or normal-phase material. The use of
Luna base silica results in overall phase reproducibility and performance.
Benzalkonium Chloride, Nortriptyline HCl Capsules, Prednisolone, Tetracaine,

Quinapril tablets
L11
L20
Phenyl-Hexyl
3, 5, 10, 10-PREP, 15 m
A phenyl phase which employs a hexyl alkyl linker as opposed to the traditional propyl chain. Offers great stability as well as alternative selectivity.
Oxacillin, Captopril, Chlorpheniramine, Pseudoephedrine, Methadone Hydrochloride

Oral Concentration
HILIC
3, 5 m
HILIC phase that provides excellent selectivity for polar compounds; and
improved MS sensitivity with low bleed.
Drug metabolites, Water soluble vitamins, Melamine, Cyanuric acid,

Metanephrine, Normetanephrine
L43
PFP(2)
3, 5 m
A pentafluorophenyl phase that provides excellent selectivity for aromatic
compounds from influence of flourine substitution on phenyl ring. Multiple
retention mechanisms. Orthogonal selectivity to traditional C18 phases.
Positional isomers, Geometric isomers, Taxanes, Aflatoxins
Luna
Description
C18(2)
Phase
HPLC
Luna Bonded Phase Selectivity Chart
Try Gemini-NX for even longer column lifetimes (see page 168).
Phenomenex
193
Luna
Luna Silica
Luna Silica(2)
pH Stability:
Particle Size:
Phase:
Application:
A Backbone and Phase Designed for

Long Column Lifetimes
USP: L3
2.0 7.5
3 m, 5 m, 10 m, 10 m-PREP, and 15 m
Unbonded silica
Polar compounds
Luna columns excellent performance is not simply the result of

ultra-pure metal-free silica (99.99% purity). Meticulous care is
given to the quality control of surface smoothness, pore structure
and pore consistency to ensure particles of uniform structure and
enhanced mechanical strength. Either bonded or unbonded, Luna
silica produces highly advanced HPLC columns:
Low percentage of fines from damaged silica leading
to lower backpressures and enhanced column
performance and lifetimes
High column bed stability enhanced by particle
shape uniformity
Compare Surface Smoothness
SFC
APPROVED
Luna silica is extremely smooth and spherical. For bonded phases,

this provides a uniform bonding surface for consistent and even
bonded phase coverage. The likelihood of silica particle shearing
and breakage during bonding and packing is very low; thus, Luna
columns have high efficiencies and long column lifetimes.
Natural Products (Kava Kava)

Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Sample:
Luna 5 m Silica(2)
150 x 4.6 mm
00F-4274-E0
Hexane/Dioxane (85:15)
1.5 mL/min
UV @ 230 nm
1. Dihydrokawain
2. Yangonin
3. Kawain
4. Dihydromethysticin
5. Methysticin
Waters Symmetry
5 m C18
LUNA
HPLC
Recommended for preparative and bulk packing into DAC

systems, see page 344 for more information
Phenomenex Luna
5 m C18
Incredible Silica Smoothness
4
2
App ID 1138
194
10
20
min

5 m SB-C18
Phenomenex
Luna
Luna C18(2), C8(2), C5
Luna C18(2)
Your Starting Point for Reversed

Phase Methods
The Luna column has found a place as one of the worlds top reversed phase columns because it provides a measurable improvement over many HPLC columns for two important chromatographic
properties: resolution and peak shape. The high efficiencies and
bonded phase surface coverage provide for sharp peaks. The result:
pH Stability:
Particle Size:
Phase:
Application:
Strength:
USP: L1
Luna C8(2)
Free exposed silanols virtually eliminated by complete

bonding and endcapping
pH Stability:
Particle Size:
Phase:
Application:
Strength:
Sharp peak shape for good method sensitivity

pH stable from 1.5 to 10.0 for over 10000 hours
C18 Column Comparison**
LC/MS
Certified
1.5-9.0*
2.5 m, 3 m, 5 m, 10 m, 10 m-PREP, and 15 m
C18, endcapped
Small molecules
Wide pH stability provides longer column lifetime and greater method
flexibility
USP: L7
LC/MS
Certified
1.5-9.0*
3 m, 5 m, 10 m, 10 m-PREP, and 15 m
C18, endcapped
Small molecules when less retention and greater speed is desired
Lower silanol activity than C18(2) phase plus wide pH stability for longer
column life and greater method flexibility
Luna 5 m C18(2)
15792
mAU
Salicylic acid
Peak Area: 4033
Luna C5
pH Stability:
Particle Size:
Phase:
Application:
Strength:
600
400
LC/MS
Certified
SFC
APPROVED
1.5-9.0*
5 m, 10 m
C5, endcapped
Small molecules when less retention and greater speed is desired
Greater hydrolytic and pH stability compared to most C4 phases
* pH range is 1.5-10 under isocratic conditions. pH range is 1.5-9 under gradient conditions.
HPLC
200
App ID 15792
Luna
0
0
10
12
14
16
18
min
Waters SunFire 5 m C18
15793
mAU
600
Salicylic acid
Peak Area: 513
400
Pyridine Peak Asymmetry Comparison**
Luna 5 m C18 (2)
App ID 15793
2
200
10
12
14
16
** The comparative data presented here may not be representative for all applications.
Aromatic Acids
Columns: Luna 5 m C18(2) 100
SunFire 5 m C18 100
Mobile Phase: 20 mM Phosphate buffer, pH 2.5 / Acetonitrile (75:25)
Sample: 1. p-Hydrozybenzoic acid 3. Salicylic acid
2. Benzoic acid
4. p-Toluic acid
Phenomenex
Sigma-Aldrich Biotechnology
Supelcosil LC-18-BD
Waters
Spherisorb 5 m ODS (2)
0
0
Sigma-Aldrich Biotechnology
Supelcosil 5 m ABZ
18
min
ZORBAX 5 m SB-C18
G.L. Sciences
Inertsil ODS (2)
Waters
Symmetry 5 m C18
Comparison of 7 different 5 m reversed phase columns. This survey

measures the degree of silanol activity on the surface of each silica. In this
survey, Luna 5 m C18(2) material demonstrates the lowest silanol activity.
195
Luna
Luna C18(2), C8(2), C5 (contd)

Steroids
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Sample:
Polynuclear Aromatic Hydrocarbons: EPA Method 8310

Column:
Dimensions:
Part No.:
Mobile Phase:
Luna 5 m C18(2)
150 x 4.6 mm
00F-4252-E0
0.1 % H3PO4 /Acetonitrile/Methanol (54:35:11)
0.75 mL/min
UV @ 254 nm
1. Hydrocortisone
3. 11-a-Hydroxyprogesterone
2. Corticosterone
4. Cortisone Acetate
5. 11-Ketoprogesterone
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:
1
2
Luna 5 m C18 (2)

250 x 4.6 mm
00G-4252-E0
A: Water
B: Acetonitrile
A/B (25:75) to 100 % B in 25 min
2.0 mL/min
22 C
UV @ 254 nm
1. Naphthalene
5. Anthracene
2. Acenaphthylene
6. Fluoranthene
3. Fluorene
7. Pyrene
4. Phenanthrene
App ID 1185
10
12
14
16 min
App ID 14269
5
4
9. Chrysene
10. Benzo[e]pyrene
13. Benzo[a]pyrene
16. Indeno[1,2,3-c,d]pyrene
Saw Palmetto Berry, p-Bromophenacyl Esters
SFC
HPLC
APPROVED
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:
Luna 3 m C8(2)
150 x 4.6 mm
00F-4248-E0
Acetonitrile/Water (87:13)
1.5 mL/min
25 C
UV @ 254 nm
1. Capric Acid
5. Linoleic Acid
2. Lauric Acid
6. Palmitic Acid
3. Linolenic Acid
7. Oleic Acid
4. Myristic Acid
8. Stearic Acid
Acetaminophen, USP Method

Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Sample:
2
7
Luna 5 m C18(2)
150 x 4.6 mm
00F-4252-E0
Water/Methanol/Acetic Acid (69:28:3)
1.5 mL/min
UV @ 275 nm
1. Acetaminophen
2. Caffeine
3. Benzoic Acid
LUNA
5
3
10
20
App ID 1293
App ID 1072
25 min
10
12
min
Pharmaceutical Preservatives
Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:
Luna 5 m C5
150 x 4.6 mm
00F-4043-E0
A: 0.5 % Acetic acid in water/acetonitrile (80:20)
B: 0.5 % Acetic acid in water/acetonitrile (20:80)
A/B (100:0) to A/B (0:100) in 30 min
1 mL/min
25 C
UV @ 254 nm
1. Propylparaben impurity
2. Benzyl alcohol
3. Phenol
4. Benzoic acid
5. Methylparaben
6. Benzaldehyde
7. Ethylparaben
8. Propylparaben
Adenine Family
Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:
Luna 5 m C18(2)
150 x 4.6 mm
00F-4252-E0
A: 20 mM Ammonium acetate, pH 4.5
B: Acetonitrile
A/B (97:3) to (60:40) in 10 min
1 mL/min
Ambient
UV@ 260 nm
1. ATP
4. Adenine
2. ADP
5. Adenosine
3. AMP
5
4
1
App ID 1141
App ID 15756
196
10
12
min
Phenomenex
Luna
Luna Phenyl-Hexyl
Luna Phenyl-Hexyl
Engineered for Stability

Luna Phenyl-Hexyl columns provide separations not achievable
on C18 or C8 columns; such as increased retention for polar,
aromatic compounds as well as reversals in analyte elution order.
Luna Phenyl-Hexyl columns are a reproducible, extremely stable
phenyl phase. Most phenyl phases use a short propyl (3 carbon)
linker, which limits phase stability. The Phenyl-Hexyl bonded
phase employs a phenyl ring with a hexyl (6 carbon) linker and
is densely bonded to Luna silica surface. Dense bonding and
the hexyl linker reduce bonded phase hydrolysis and increases
chemical stability. The result:
pH Stability:
Particle Size:
Phase:
Application:
Strength:
USP: L11
LC/MS
Certified
1.5-9.0*
3 m, 5 m, 10 m, 10 m-PREP, and 15 m
Phenyl with Hexyl (C6) linker, endcapped
Non-polar compounds
Aromatic selectivity enhanced by higher hydrophobicity due to hexyl linker
* pH range is 1.5-10 under isocratic conditions.

pH range is 1.5-9 under gradient conditions.
Highly reproducible and stable phenyl phase

Dual selectivity of both phenyl phase and a short alkyl
phase (C5 or C8)
Excellent retention of aromatic and polar,
amine compounds
Recommended for US EPA Method 8330B for explosives
analysis
SFC
1.5 to 10 pH stability for over 10000 hours
APPROVED
Chromatographic Comparisons of Phenyl Columns**
Luna 5 m Phenyl-Hexyl
Peaks 7, 8
Some Absorption
HPLC
Waters Spherisorb 5 m Phenyl
10
15
App ID 1201
Luna
App ID 10822
2
1
20 min
Agilent Technologies Zorbax 5 m SB-Phenyl
Peaks 4, 5, 6
Poor Resolution
Peaks 7, 8
Total Absorption
App ID 10823
App ID 10821
Peaks 1, 2
Poor Resolution
Agilent Technologies Zorbax 5 m Phenyl
Antibacterials
Mobile Phase: A: 20 mM KH2PO4, pH 2.5
B: Acetonitrile
Gradient: Start A/B (80:20) to A/B (75:25) in
5 min, go to A/B (55:45) in 15 min
Sample: 1. Carbadox
4. Oxolinic Acid
2. Thiamphenicol
5. Sulfadimethoxine
3. Furazolidone
6. Sulfaquinoxaline
7. Nalidixic Acid
8. Piromidic Acid
Phenomenex
197
Luna
Luna CN (cyano)
Luna CN
Proven Reproducibility
For carboxyl, carbonyl and amine containing compounds, Luna
CN columns offer a unique polar selectivity in reversed phase and
normal phase mode. Luna CN columns provide sharp peaks and
great reproducibility run-to-run, column-to-column and batch-tobatch. The smooth silica allows for a more uniform bonding with
improved resistance to bonded phase hydrolysis to produce one
of the most stable CN phases. The result:
pH Stability:
Particle Size:
Phase:
Application:
USP: L10
1.5-7.0
3 m, 5 m, and 10 m
Cyano, endcapped
Compounds with COOH, CO, NH2, NHR2, or NR2
Strength: Improved reproducibility for more consistent results

run-to-run, column-to column, batch-to-batch
Improved peak shapes

One of the most stable CN columns under reversed phase
or normal phase conditions.
pH stable from 1.5 to 7.0
CN Column Comparsion**
Luna 5 m CN
SFC
APPROVED
Luna 5 m CN
150 x 4.6 mm
A: Hexane, B: Methylene chloride/Methanol(80:20), A/B (80:20)
2.0 mL/min
UV @ 254 nm
1.0 L
Ambient
1. Hydrocortisone
2. Prednisone
3. Cortisone
4. Hydrocortisone Acetate
App ID 3464
Column:
Dimensions:
Mobile Phase:
Flow Rate:
Detection:
Injection:
Temperature:
Sample:
App ID 3471
HPLC
Waters Spherisorb 5 m CN
App ID 15725
Luna 5 m CN
150 x 4.6 mm
A: Hexane, B: Methylene chloride/Methanol(80:20), A/B (95:5)
1.0 mL/min
5 L
UV @ 254 nm
Ambient
1. Dimethyl phthalate
2. Diethyl phthalate
3. Dibutyl phthalate
4. Dioctyl phthalate
Agilent Technologies Zorbax 5 m SB-CN
App ID 15726
LUNA
Column:
Dimensions:
Mobile Phase:
Flow Rate:
Injection:
Detection:
Temperature:
Sample:
App ID 3466
Column-to-Column Reproducibility
Reversed Phase Conditions

Column:
Dimensions:
Mobile Phase:
Flow Rate:
Detection:
Sample:
Luna 5 m CN
150 x 4.6 mm
A: Acetonitrile, B: 20 mM Potassium phosphate pH 4.0, A/B (35:65)
1.0 mL/min
UV @ 210 nm
Tricyclic Antidepressants
1. Doxepin
2. Desipramine
3. Nortriptyline
4. Amitriptyline
5
1
5. Protriptyline
4
App ID 12958
10
Normal Phase Conditions
12
14
16
18

Mobile Phase: A: Hexane, B: Methylene chloride/Methanol (80:20), A/B (99:1))
Sample: Phthalate Esters
1. Di-n-octyl phthalate
2. Bis (2-Ethylhexyl) phthalate
3. Butylbenzyl phthalate
4. Di-n-butyl phthalate
5. Diethyl phthalate
6. Dimethyl phthalate
min
198
Phenomenex
Luna
Luna NH2 (amino)
Luna NH2
USP: L8
pH Stability:
Particle Size:
Phase:
Application:
Strength:
Developed for Ruggedness

Luna NH2 columns retain hydrogen-bonding compounds under
three separation modes: Reversed Phase, Normal Phase, and IonExchange. Luna NH2 columns provide reproducible retention and
selectivity with improved column lifetime. Amino columns can be
problematic as the bonded phase easily hydrolyses off the silica,
shortening retention time over the life of the column. The bonded
phase stability of Luna NH2 columns are illustrated by 1.5 to 11.0
pH stability and 100 % aqueous mobile phase stability. The result:
1.5-11.0
3 m, 5 m, and 10 m
Amino
Compounds with COOH, CO, NH2, NHR2, or NR2
Sugars by reversed phase, steroids by normal
phase, oligonucleotides by ion exchange
Increased bonded phase stability improves

reproducibility and column lifetime
Excellent retention of simple sugars, complex
sugars, sugar alcohols and other hydrogen-bonding
compounds under reversed phase, normal phase,
ion-exchange conditions
12-18mer poly-DT Oligonucleotide
pH stable from 1.5 to 11.0
Column:
Dimensions:
Part No.:
Mobile Phase:
Stable in 100 % aqueous mobile phases
Stability in 100 % Aqueous Mobile Phase
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:
Luna 5 m NH2
250 x 4.6 mm
00G-4378-E0
20 mM Potassium Phosphate Buffer pH 2.7
1.5 mL/min
UV @ 254 nm
Ambient
2.5 L
Continuously flushed at 1.0 mL/min using 100 % 20 mM Potassium
Phosphate Buffer pH 2.7 between injections
4 5
6
SFC
APPROVED
HPLC
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detector:
Temperature:
Injection:
Conditions:
Luna 5 m NH2
150 x 4.6 mm
00F-4378-E0
A: 20 mM Sodium Phosphate, 10 % Acetonitrile, pH 7.0
B: 20 mM Sodium Phosphate, 10 % Acetonitrile,
1 M Sodium Chloride, pH 7.0
A/B (75:25) to A/B (5:95) in 50 minutes
1 mL/min
40 C
UV @ 260 nm
12-18mer poly-DT oligonucleotide standard
App ID 14628
Luna
20
30
40 min
Simple Sugars
Luna 5 m NH2
250 x 4.6 mm
00G-4378-E0
Hexane/Ethanol (85:15)
2 mL/min
22 C
UV @ 240 nm
1. 11-Ketoprogesterone
3. Cortisone Acetate
4. Prednisolone 21-Acetate
5. Cortisone
6. Prednisolone
App ID 14299
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:
Phenomenex
Luna 5 m NH2
250 x 4.6 mm
00G-4378-E0
3 mL/min
40 C
RI
1. Xylose
5. Maltose
2. Fructose
6. Melezitose
3. Glucose
7. Raffinose
4. Sucrose
App ID 14285
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:
Steroids
199
Luna
Luna SCX (strong cation exchange)
Luna SCX
Develop Robust Methods

Luna SCX columns provide excellent resolution and peak shape
of basic, cationic compounds. However, most SCX columns
show poor peak shape and bad resolution causing many chromatographers to ignore this important phase for small molecule
method development, until now. Luna SCX columns contain a
benzene sulfonic acid ligand providing ion-exchange reversed
phase, and aromatic interactions. Such interaction make Luna
SCX columns great as a first dimension of 2D LC applications as
well as improved resolution for small molecules. The result:
Resolving power and sharp peak shape to separate
complex cationic/basic and nitrogen containing
compounds
Column:
Dimensions:
Part No.:
Mobile Phase:
Benzene sulfonic acid ligand provides mixed-mode

interaction improving separation for 2D peptide
applications
Gradient:
Flow Rate:
Temperature:
Detection:
Injection Volume:
Sample:
SFC
APPROVED
Luna 5 m SCX
150 x 4.6 mm
00F-4398-E0
50 mM KH2PO4, pH 2.5/Acetonitrile (35:65)
1 L
1.5 mL/min
UV @ 210 nm
Dissolve 1 part Childrens Tylenol Cold in 10 parts Methanol
1. Acetaminophen
2. Pseudoephedrine
3. Dextromethorphan
4. Chlorpheniramine
Luna 5 m SCX
150 x 4.6 mm
00F-4398-E0
A: 20 mM Potassium Phosphate, 25 % Acetonitrile, pH 2.5
B: 20 mM Potassium Phosphate, 25 % Acetonitrile, 400 mM
Potassium Chloride, pH 2.5
1 mL/min
35 C
UV @ 215 nm
2 L (5 g on column)
Peptide Mixture - Substance P
1. Fragment 5-11 (+1)
2. Fragment 4-11 (+1)
3. Fragment 2-11 (+2)
4. Fragment 1-9 (+3)
5. Intact (+3)
6. (ARG-PHE-TRP-LEU) (+3)
6
5
3
4
App ID 14765
HPLC
Childrens Tylenol Cold Syrup
LUNA
2.0-7.0
5 m and 10 m
Benzene Sulfonic Acid, Strong Cation Exchange
Amine and polyamine containing compounds
Guaranteed to provide sharper peak shape and better
resolution compared to traditional SCX columns
Peptides
5 and 10 m columns and bulk media for analytical

through preparative separations
Column:
Dimensions:
Part No.:
Mobile Phase:
Injection Volume:
Flow Rate:
Detection:
Sample Prep:
Sample:
USP: L9
pH Stability:
Particle Size:
Phase:
Application:
Strength:
10
15
20
25
30
35
40
min
Tryptic Digest of Bovine Cytochrome c
App ID 14555
Column:
Dimensions:
Part No.:
Mobile Phase:
10 min
Gradient:
Flow Rate:
Temperature:
Detection:
Injection Volume:
Sample:
Luna 5 m SCX
150 x 4.6 mm
00F-4398-E0
A: 20 mM Potassium Phosphate, pH 2.5 / 25 % Acetonitrile
B: 20 mM Potassium Phosphate, pH 2.5 /
25 % Acetonitrile / 350 mM Potassium Chloride
100 % A to 100 % B in 50 minutes
1 mL/min
35 C
UV @ 215 nm
50 L (20 g on column)
Bovine Cytochrome c trypsin digest
App ID 14767
SCX Method Development and pH: The standard operating pH range

for Luna SCX columns is 2.0 to 7.0. Most SCX methods are
typically run between pH 2.0 and 5.0 for optimal performance.
This ensures that nitrogen-containing analytes, especially those
with adjacent conjugated system are protonated. Running in
highly acidic (pH < 2.0) or basic (pH > 7.0) mobile phases may
cause this phase to undergo degradation, as is common for all
silica-based SCX phases.
0
200
10
15
20
25
30
35
40
45 min
Phenomenex
Luna
Luna HILIC
Increase MS Sensitivity and Retention for
Polar Compounds
Hydrophilic Interaction Liquid Chromatography (HILIC) is a separation mode. Separation is achieved through the partitioning of
polar solutes from the high concentration, water-miscible, organic
mobile phase into the hydrophilic surface environment. Polar solutes exhibit increased retention and elute in the order of increasing
hydrophilicity.
Finally, reproducible, robust HILIC separations!
Increase laboratory throughput and productivity

ACN
ACN
ACN
ACN
H 2O
ACN
H2O
H2O
H 2O
H 2O
OH
ACN
O
10
12min
Luna 5 m HILIC
150 x 4.6 mm
00F-4450-E0
A: Acetonitrile
B: Water
C: 100 mM Ammonium Acetate, pH 5.8
A/B/C (90:5:5) for 2.5 min to A/B/C (50:45:5) in 7.5 min, hold for
2.5 min. Re-equilibrate @ A/B/C (90:5:5) for 7.5 min
2.0 mL/min
UV @ 260 nm
+
1. p-Aminobenzoic Acid pKa 4.7, H pKa 2.7 logP 0.83
+
2. Nicotinamide H pKa 3.35 logP 0.37
3. Riboflavin pKa 10.2 logP 1.46
4. Nicotinic Acid pKa 4.7, H+pKa3.0 logP 0.36
+
5. Pyridoxine H pKa 5.6, pKa 8.6 logP 0.77
+
6. Thiamine H pKa 5.5 logP 4.6
7. Ascorbic Acid pKa 4.1, 11.2 logP 1.85
8. Cyanocobalamin pKa 1.59 logP 0.90
9. Folic Acid pKa 2.7, 4.1, 8.9 logP 0.02
SFC
APPROVED
H 2O
H 2O
H 2O
Luna
H 2O
ACN
O
H 2O
H 2O
O
H 2O
H2O
O
O
HPLC
H 2O
O
O H 2O
OH
OH
H2O
OH
OH
ACN
OH
Flow Rate:
Detection:
Sample:
ACN
ACN
H 2O
Gradient:
ACN
OH
OH
ACN
ACN
ACN
ACN
ACN
7
1
H 2O
ACN
ACN
ACN
ACN
OH
ACN
to
Column:
Dimensions:
Part No.:
Mobile Phase:
Increase mass spectrometry sensitivity
H 2O
Made for retention of polar compounds
ACN
Vitamin Mix on Luna HILIC

Vitamins provide an excellent platform to demonstrate the benefits
of HILIC. The effect of increased polar compound retention can be
easily seen in this application.
App ID 16430
Luna HILIC columns retain a water-enriched layer on the surface

of the silica. This water layer facilitates the transfer of polar compounds onto the stationary phase for increased retention.
H 2O
H 2O
Ganciclovir & Acyclovir on Luna HILIC

HILIC uses a solvent system that is the reverse of what is common
in reversed phase, thus peak elution order is reversed on the Luna
HILIC column.
Polar / Hydrophilic compounds that are particularly difficult to retain, even on polar-embedded reversed phase columns, will enjoy
maximum retention on Luna HILIC columns.
Luna HILIC
2.1e4
Polar Embedded C18
2.0e4
1.9e4
Column:
Dimensions:
Part No.:
Mobile Phase:
Luna 3 m HILIC
100 x 2.0 mm
00D-4449-B0
Acetonitrile/100 mM
Ammonium Formate,
pH 3.2 (90:10)
Sample: 1. Acyclovir
2. Ganciclovir
1
2.1e4
2.0e4
1.9e4
More Polar Compound

= More Retention!
1.8e4
1.7e4
1.6e4
1.5e4
1.4e4
1.3e4
1.2e4
1.1e4
1.0e4
9000.0
8000.0
Column: Synergi 4 m
Fusion-RP 80
1.5e4
Part No.: 00D-4424-B0
1.4e4
Mobile Phase: Acetonitrile/10 mM
1.3e4
Ammonium
1.2e4
1.1e4
Formate, pH 3.2 (3:97)
1.0e4
9000.0
8000.0
Sample: 1. Acyclovir
7000.0
2. Ganciclovir
1.7e4
1.6e4
6000.0
2
5000.0
App ID 16440
6000.0
5000.0
4000.0
3000.0
2000.0
1000.0
0.2
0.4
0.6
Phenomenex
0.8
1.0
1.2
1.4
1.6
1.8
2.0
2.2
2.4
2.6
2.8
min
4000.0
App ID 16441
7000.0
0.0
1.8e4
3000.0
2000.0
1000.0
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
2.2
2.4
2.6
2.8
min
201
Luna
Luna HILIC (contd)
0.0
Improved MS Sensitivity
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
2.2
2.4
2.6
2.8
3.0
3.2
3.4 min
The increased retention in HILIC allows elution of the analytes outside the suppression region and thus increased detector sensitivity.
In addition, the Luna HILIC column also resolved the compounds with the reverse order of that seen in reversed phase LC.
Nicotine and Metabolites
Luna HILIC
Cotinine
Peak Area
123,700
Increase in sensitivity
Nicotine
Peak Area
82,900
Nornicotine
Peak Area
69,200
App ID 16335
0.5 ng on column
SFC
0.0
APPROVED
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
2.2
2.4
2.6
2.8
3.0
3.2
C18
4.8e4
4.6e4
4.4e4
4.2e4
4.0e4
3.8e4
3.6e4
3.4e4
3.2e4
3.0e4
2.8e4
2.6e4
2.4e4
2.2e4
2.0e4
1.8e4
1.6e4
1.4e4
1.2e4
1.0e4
8000.0
6000.0
4000.0
2000.0
0.0
3.4 min
HPLC
|
LUNA
Luna 5 m HILIC
100 x 2.0 mm
00D-4450-B0
Acetonitrile/100 mM Ammonium
Formate, pH 3.2 (90:10)
Sample: 1. Cotinine (MRM: 177/80)
2. Nicotine (MRM: 163/80)
3. Nornicotine (MRM: 149/80)
Elutes in
ion suppression zone!
Nornicotine
Peak Area
4,281
Nicotine
Peak Area
4,849
2
0.5 ng on column
1
0.2
0.4
0.6
Column:
Dimensions:
Part No.:
Mobile Phase:
Column:
Dimensions:
Part No.:
Mobile Phase:
4.8e4
4.6e4
4.4e4
4.2e4
4.0e4
3.8e4
3.6e4
3.4e4
3.2e4
3.0e4
2.8e4
2.6e4
2.4e4
2.2e4
2.0e4
1.8e4
1.6e4
1.4e4
1.2e4
1.0e4
8000.0
6000.0
4000.0
2000.0
0.0
Cotinine
Peak Area
97,400
Gradient:
Flow Rate:
Detection:
Sample:
App ID 16345
0.8
1.0
1.2
1.4
1.6
1.8
2.0
2.2
2.4
2.6
2.8
3.0
3.2
3.4 min
Gemini 5 m C18
100 x 2.0 mm
00D-4435-B0
A: Water with 0.1 % v/v Formic Acid
B: Acetonitrile with 0.1 % v/v Formic Acid
A/B (97:3) hold 1 min, to (70:30) in 2 min, to (97:3)
in 0.01 min, hold for 2.5 min
0.4 mL/min
Mass Spectrometer (MS)
1. Nornicotine (MRM: 149.1/80.1)
2. Nicotine (MRM: 163.3/105.9)
3. Cotinine (MRM: 177.3/80.1)
2
5
to
4
Unique HILIC Selectivity
6
3
Not all HILIC columns are alike, Luna HILIC columns delivers on the exacting standards you have come to trust from the Luna
0
2
4
6
8
10
min
product line.
1
HILIC Column Comparison**
Luna 5 m HILIC
Waters Atlantis 5 m HILIC

4
0.8
1.0
1.2
1.4
1.6
1.8
2.0
Baseline resolution
2.4
2.6 peaks
2.8 3.0 3.2
of all
2.2
App ID 16337
4
6
3
2
1
10
min
10
min
10
min
Peak 6
(Thiamine-no elution)
to
5
4
3
2
1
5
to
to
to
3.4 min
App ID 16432
0.6

4
Column: As noted
Dimensions: 150 x 4.6 mm 3
Mobile
Phase: Acetonitrile/100
mM6 Ammonium
2
4
Formate, pH 3.2 (90:10)
1Flow Rate: 1.0 mL/min
2
t
Sample: 1. PABA
2. Nicotinamide5
3. Riboflavin
3
4. Nicotinic Acid
5. Pyridoxine
2
4
6
6. Thiamine
4
6
SeQuant 5 m ZIC-HILIC
3
0
10
10
min
10
min
Peak 6
(Thiamine-no elution)
min
2
App ID 16431
0.2 1 0.42
to
to
3
0
10
min
to
** The comparative data presented here may not be representative for all applications.
202
Phenomenex
3
0
10
min
Luna
Luna PFP(2)
Luna PFP(2) Selectivity is
achieved through 4 of the 5
mechanisms of interaction
Hydrogen Bonding
Powerful Selectivity for Reversed Phase

Methods
Luna PFP(2) columns provide remarkable selectivity for highly polar
compounds, complex natural products, isomers, and other closely
related compounds. This is achieved by using a pentafluorophenyl
with a propyl linkage which provides multiple retention mechanisms
different to other reversed phase media.
Dipole-Dipole Interactions
Aromatic and - Interactions
Hydrophobic
Achieve excellent selectivity using four mechanisms of

solute/stationary phase interactions
Extremely discerning for halogenated, aromatic and
conjugated compounds
A typical alkyl phase (C18, C8) achieves selectivity through only 1 mechanism of interaction.
Provides orthogonal selectivity even using traditional

reversed phase mobile phase systems
Positional Isomers of Halogenated Phenols

Halogens can radically increase the polarity of a compound, thus decreasing typical retention characteristics. Luna PFP(2) columns retain,
discriminate, and separate halogens easily.
Luna 3 m PFP(2)
5
Luna 3 m C18(2)
SFC
APPROVED
16297
5
3
1
2
9
4
3
Coelution!
HPLC
9 & 10
Baseline
separation!
8
6
10
10
12
14
min
10
12
14
Luna
App ID 16296
App ID 16297
min
Columns: Luna 3 m PFP(2)

Luna 3 m C18(2)
Part Nos.: 00F-4447-E0
00F-4251-E0
Flow Rate: 1 mL/min
Temperature: 22 C
Sample: 1. 2,3-Dimethylphenol
10. 2,4-Dibromophenol
Phenomenex
203
Luna
Luna PFP(2) (contd)

Positional Isomers of Methylacetophenone
Positional changes on an analyte of interest may effect the compounds dipole moment. This change can be readily noticed by the highly
electronegative fluorine (F) atom and other retention mechanisms.
Luna 3 m PFP(2)
16298
Luna 3 m C18(2)
16299
3
4 mechanisms of
interaction provide good
resolution of isomers!
1 mechanism of
interaction unable
to resolve isomers!
App ID 16299
App ID 16298
SFC
APPROVED
10
12
14
16
18
min
10
12
14
16
18
min

Mobile Phase: Water/ Methanol (50:50)
Flow Rate: 1 mL/min
Temperature: 22 C
Sample: 1. o-Methylacetophenone
2. m-Methylacetophenone
3. p-Methylacetophenone
LUNA
HPLC

Luna 3 m C18(2)
00F-4251-E0
Catechins
The presence of the aromatic benzene ring in Luna PFP(2) columns increase the relative attraction between the stationary phase and
aromatic analytes, leading to increased retention for these types of compounds.
Luna 3 m PFP(2)
Luna 3 m C18(2)
16295
16294
1
1
Baseline
resolution!
7
56
3
10
12

Luna 3 m C18(2)
00F-4251-E0
204
14
0
0
App ID 16295
5 6
3
App ID 16294
10
12
14
min
min

Flow Rate: 1 mL/min
Sample: 1. Gallic acid

2. Epigallo catechin
3. Catechin
4. Epicatechin
5. Epigallocatechin gallate
6. Gallocatechin gallate
7. Epicatechin gallate
8. Catechin gallate
Phenomenex
Luna

FULL REFUND.
2.5 m High Speed Technology (HST) Columns (mm)
Phase
30 x 2.0
50 x 2.0
Luna 2.5 m C18(2)-HST
00A-4446-B0
00B-4446-B0
100 x 2.0
50 x 3.0
100 x 3.0
00D-4446-B0
00B-4446-Y0
00D-4446-Y0
For information about HST Columns, contact your Phenomenex technical consultant or local distributor.
3 m and 5 m Capillary Columns (mm)
Phases
50 x 0.30
150 x 0.30
3 m C8(2)
3 m C18(2)
5 m C8(2)
5 m C18(2)
5 m Phenyl-Hexyl
00B-4248-AC
00B-4251-AC
00B-4252-AC
00B-4257-AC
00F-4248-AC
00F-4251-AC
00F-4249-AC
00F-4252-AC
250 x 0.30
50 x 0.50
150 x 0.50
250 x 0.50
00G-4251-AC
00G-4249-AC
00G-4252-AC
00B-4248-AF
00B-4251-AF
00B-4252-AF
00B-4257-AF
00F-4248-AF
00F-4251-AF
00F-4249-AF
00F-4252-AF
00G-4251-AF
00G-4252-AF
Guard Columns (mm)

20 x 0.30
20 x 0.50
03M-4248-AC
03M-4251-AC
03M-4249-AC
03M-4252-AC
03M-4248-AF
03M-4251-AF
03M-4249-AF
03M-4252-AF
For Fused Silica Capillary Adapter, see p. 360

MercuryMS LC/MS Cartridges (mm)
Qty Pricing
3 m
Luna
Luna
5 m
Luna
Luna
10 x 2.0
1@
@ 270
250 /ea
3@
@ 265
240 /ea
5@
@ 260
225 /ea
10
10@
@ 215
250 /ea
/ea
10 x 4.0
11 @
@ 250
270 /ea
33 @
@ 240
265 /ea
55 @
@ 225
260 /ea
10
10@
@ 215
250/ea
/ea
20 x 2.0
11 @
@ 250
270 /ea
33 @
@ 240
265 /ea
55 @
@ 225
260 /ea
10
10@
@ 215
250/ea
/ea
20 x 4.0
1@
@ 270
250 /ea
3@
@ 265
240 /ea
5@
@ 260
225 /ea
10
10@
@ 215
250 /ea
/ea
Columns (mm)
20 x 2.0
20 x 4.0
Phase
C18(2)
C8(2)
00N-4251-B0-CE
00N-4248-B0-CE
00N-4251-D0-CE
00N-4248-D0-CE
00M-4251-B0-CE
00M-4248-B0-CE
00M-4251-D0-CE
00M-4248-D0-CE
00M-4251-B0
00M-4248-B0
C18(2)
C8(2)
00N-4252-B0-CE
00N-4249-B0-CE
00M-4252-B0-CE
00M-4249-B0-CE
00M-4252-D0-CE
00M-4249-D0-CE
These convenient screening kits allow quick,

easy and economical evaluation of multiple phases. Each kit contains one cartridge
of each available phase and a standard holder.
Available in either 3m or 5m Luna with
2.5m Synergi.
Luna
Standard Cartridge Holders

Part No.
Description
CH0-5846
10 mm standard holder
CH0-5845
MercuryMS Screening Kits
Standard Holder
Direct-Connect Cartridge Holders
Part No.
Description
CH0-7187
10 mm direct-connect holder
CH0-7188
Direct-Connect Holder
00M-4251-D0
00M-4248-D0
HPLC
MercuryMS Cartridge Holders
SFC
APPROVED
Screening Kits
Price
Price
Kit A includes 1 ea of:

Luna 3 m C18(2) Cartridge
Synergi 2.5 m Max-RP Cartridge
Synergi 2.5 m Hydro-RP Cartridge
20 mm Standard Cartridge Holder
Kit B includes 1 ea of:
20 x 2.0 mm Kit
KH0-7333
20 x 4.0 mm Kit
KH0-7334
KH0-7335
KH0-7336
3 m Microbore and Minibore Columns (mm) SecurityGuard Cartridges (mm)

Phases
50 x 1.0
150 x 1.0
30 x 2.0
50 x 2.0
100 x 2.0
150 x 2.0
4 x 2.0*
/10pk
Silica(2)
00A-4162-B0
00B-4162-B0
00D-4162-B0
00F-4162-B0
AJ0-4347
C8(2)
00B-4248-A0
00F-4248-A0
00A-4248-B0
00B-4248-B0
00D-4248-B0
00F-4248-B0
AJ0-4289
C18(2)
00B-4251-A0
00F-4251-A0
00A-4251-B0
00B-4251-B0
00D-4251-B0
00F-4251-B0
AJ0-4286
CN
00A-4254-B0
00B-4254-B0
00D-4254-B0
00F-4254-B0
AJ0-4304
Phenyl-Hexyl
00B-4256-A0
00A-4256-B0
00B-4256-B0
00D-4256-B0
00F-4256-B0
AJ0-4350
NH2
00F-4377-A0
00A-4377-B0
00B-4377-B0
00D-4377-B0
00F-4377-B0
AJ0-4301
HILIC
00B-4449-B0
00D-4449-B0
00F-4449-B0
AJ0-8902
PFP(2)
00A-4447-B0
00B-4447-B0
00D-4447-B0
00F-4447-B0
AJ0-8326
for ID: 2.0-3.0 mm
Phenomenex
205
Luna
3 m Narrow Bore and Analytical Columns (mm)
Phases
30 x 3.0
50 x 3.0
150 x 3.0
Silica(2)
C8(2)
00A-4248-Y0 00B-4248-Y0
C18(2)
00A-4251-Y0 00B-4251-Y0
CN
00B-4254-Y0
Phenyl-Hexyl
00B-4256-Y0
NH2
00B-4377-Y0
HILIC
00B-4449-Y0
PFP(2)
00B-4447-Y0
00F-4162-Y0
00F-4248-Y0
00F-4251-Y0
00F-4254-Y0
00F-4256-Y0
00F-4377-Y0
00F-4449-Y0
00F-4447-Y0
30 x 4.6
50 x 4.6
75 x 4.6
100 x 4.6
150 x 4.6
00A-4248-E0
00A-4251-E0
00A-4254-E0
00A-4256-E0
00B-4162-E0
00B-4248-E0
00B-4251-E0
00B-4254-E0
00B-4256-E0
00B-4377-E0
00B-4447-E0
00C-4248-E0
00C-4251-E0
00C-4254-E0
00C-4256-E0
00D-4162-E0
00D-4248-E0
00D-4251-E0
00D-4254-E0
00D-4256-E0
00D-4377-E0
00D-4449-E0
00D-4447-E0
00F-4162-E0
00F-4248-E0
00F-4251-E0
00F-4254-E0
00F-4256-E0
00F-4377-E0
00F-4449-E0
00F-4447-E0
SecurityGuard
Cartridges (mm)
4 x 2.0*
4 x 3.0*
/10pk
/10pk
AJ0-4347
AJ0-4348
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
AJ0-4304
AJ0-4305
AJ0-4350
AJ0-4351
AJ0-4301
AJ0-4302
AJ0-8328
AJ0-8329
AJ0-8326
AJ0-8327
for ID: 2.0-3.0 mm
5 m Microbore and Minibore Columns (mm)

Phases
50 x 1.0
150 x 1.0
SFC
APPROVED
Silica(2)
C5
C8
C8(2)
C18
C18(2)
CN
Phenyl-Hexyl
NH2
PFP(2)
00B-4249-A0
00B-4252-A0
00B-4257-A0
00F-4249-A0
00F-4252-A0
00F-4257-A0
00F-4378-A0
250 x 1.0
30 x 2.0
00A-4043-B0
00A-4040-B0
00A-4249-B0
00A-4041-B0
00G-4252-A0 00A-4252-B0
00A-4255-B0
00G-4257-A0 00A-4257-B0
00A-4378-B0
00A-4448-B0
50 x 2.0
150 x 2.0
250 x 2.0
00B-4274-B0
00B-4043-B0
00B-4040-B0
00B-4249-B0
00B-4041-B0
00B-4252-B0
00B-4255-B0
00B-4257-B0
00B-4378-B0
00B-4448-B0
00F-4274-B0
00F-4043-B0
00F-4040-B0
00F-4249-B0
00F-4041-B0
00F-4252-B0
00F-4255-B0
00F-4257-B0
00F-4378-B0
00F-4448-B0
00G-4274-B0
00G-4249-B0
00G-4041-B0
00G-4252-B0
00G-4255-B0
00G-4257-B0
00G-4378-B0
3.2-8.0 mm
SecurityGuard
Cartridges (mm)
4 x 2.0*
/10pk
AJ0-4347
AJ0-4292
AJ0-4289
AJ0-4289
AJ0-4286
AJ0-4286
AJ0-4304
AJ0-4350
AJ0-4301
AJ0-8326
LUNA
HPLC
for ID: 2.0-3.0 mm
5 m Narrow Bore and Analytical Columns (mm)

Phases
30 x 3.0
50 x 3.0
Silica(2)
C5
C8
C8(2)
C18
C18(2)
CN
Phenyl-Hexyl
NH2
SCX
HILIC
PFP(2)
00A-4252-Y0
00A-4257-Y0
00B-4249-Y0
00B-4252-Y0
00B-4255-Y0
00B-4257-Y0
00B-4378-Y0
00B-4448-Y0
150 x 3.0
250 x 3.0
30 x 4.6
50 x 4.6
75 x 4.6
00F-4043-Y0
00F-4040-Y0
00F-4249-Y0
00F-4041-Y0
00F-4252-Y0
00F-4255-Y0
00F-4257-Y0
00F-4378-Y0
00F-4450-Y0
00F-4448-Y0
00G-4040-Y0
00G-4249-Y0
00G-4041-Y0
00G-4252-Y0
00G-4255-Y0
00G-4257-Y0
00G-4378-Y0
00A-4040-E0
00A-4249-E0
00A-4041-E0
00A-4252-E0
00A-4255-E0
00A-4257-E0
00A-4378-E0
00A-4448-E0
00B-4274-E0
00B-4043-E0
00B-4040-E0
00B-4249-E0
00B-4041-E0
00B-4252-E0
00B-4255-E0
00B-4257-E0
00B-4378-E0
00B-4398-E0
00B-4448-E0
00C-4040-E0
00C-4249-E0
00C-4041-E0
00C-4252-E0
00C-4255-E0
00C-4257-E0
00C-4378-E0

4 x 2.0*
4 x 3.0*
/10pk
/10pk
AJ0-4347
AJ0-4348
AJ0-4292
AJ0-4293
AJ0-4289
AJ0-4290
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
AJ0-4286
AJ0-4287
AJ0-4304
AJ0-4305
AJ0-4350
AJ0-4351
AJ0-4301
AJ0-4302
AJ0-4307
AJ0-4308
AJ0-8328
AJ0-8329
AJ0-8326
AJ0-8327
for ID: 2.0-3.0 mm
5 m Analytical and Semi-Prep Columns (mm)

Phases
100 x 4.6
150 x 4.6
250 x 4.6
Silica(2)
C5
C8
C8(2)
C18
C18(2)
CN
Phenyl-Hexyl
NH2
SCX
HILIC
PFP(2)
00D-4274-E0
00D-4043-E0
00D-4040-E0
00D-4249-E0
00D-4041-E0
00D-4252-E0
00D-4255-E0
00D-4257-E0
00D-4378-E0
00D-4398-E0
00D-4450-E0
00D-4448-E0
00F-4274-E0
00F-4043-E0
00F-4040-E0
00F-4249-E0
00F-4041-E0
00F-4252-E0
00F-4255-E0
00F-4257-E0
00F-4378-E0
00F-4398-E0
00F-4450-E0
00F-4448-E0
00G-4274-E0
00G-4043-E0
00G-4040-E0
00G-4249-E0
00G-4041-E0
00G-4252-E0
00G-4255-E0
00G-4257-E0
00G-4378-E0
00G-4398-E0
00G-4450-E0
00G-4448-E0
250 x 10
250 x 15
00G-4274-N0
00G-4040-N0
00G-4249-N0
00G-4041-N0
00G-4252-N0 00G-4252-AK
00G-4255-N0
00G-4257-N0
00G-4378-N0
00G-4398-N0
00G-4450-N0
00G-4448-N0
3.2-8.0 mm

4 x 3.0*
10 x 10
/10pk
/3pk
AJ0-4348
AJ0-7223
AJ0-4293
AJO-7372
AJ0-4290
AJ0-7222
AJ0-4290
AJ0-7222
AJ0-4287
AJ0-7221
AJ0-4287
AJ0-7221
AJ0-4305
AJ0-7313
AJ0-4351
AJ0-7314
AJ0-4302
AJ0-7364
AJ0-4308
AJ0-7369
AJ0-8329
AJ0-8902
AJ0-8327
AJ0-8376
for ID: 3.2-8.0 mm
9-16 mm

Ordering Information (Continued)
206
Phenomenex
Luna

FULL REFUND.
Phases
50 x 21.2
100 x 21.2
5 m
Silica(2)
C5
C8(2)
C18(2)
CN
Phenyl-Hexyl
NH2
PFP(2)
HILIC
00B-4274-P0-AX
00B-4249-P0-AX
00B-4252-P0-AX
00B-4255-P0-AX
00B-4257-P0-AX
00B-4448-P0-AX
00B-4450-P0-AX
00D-4274-P0-AX
00D-4249-P0-AX
00D-4252-P0-AX
00D-4257-P0-AX
00D-4378-P0-AX
00D-4448-P0-AX
00D-4450-P0-AX
150 x 21.2
250 x 21.2
50 x 30
100 x 30
250 x 30
aufInquire
Anfrage
00F-4274-P0-AX
00F-4043-P0-AX
00F-4249-P0-AX
00F-4252-P0-AX
00F-4255-P0-AX
00F-4257-P0-AX
00F-4378-P0-AX
00F-4448-P0-AX
00F-4450-P0-AX
00G-4274-P0-AX
00G-4252-P0-AX
00G-4255-P0-AX
00G-4378-P0-AX
00G-4448-P0-AX
00G-4450-P0-AX
00B-4274-U0-AX
00B-4249-U0-AX
00B-4252-U0-AX
00B-4257-U0-AX
00B-4448-U0-AX
00D-4274-U0-AX
00D-4249-U0-AX
00D-4252-U0-AX
00D-4255-U0-AX
00D-4257-U0-AX
00D-4448-U0-AX
00G-4274-U0-AX
00G-4252-U0-AX
00G-4448-U0-AX
00G-4450-U0-AX
50 x 30
250 x 30
aufInquire
Anfrage
50 x 50
aufInquire
Anfrage
250 x 50
aufInquire
Anfrage
Axia Packed Preparative Columns (mm) (continued)

Phases
50 x 21.2
100 x 21.2
250 x 21.2
AJ0-7229
AJ0-7840
AJ0-7839
AJ0-8220
AJ0-7841
AJ0-8162
AJ0-8377
for ID: 18-29 mm
AJ0-8312
AJ0-8302
AJ0-8301
AJ0-8311
AJ0-8303
AJ0-8309
AJ0-8378
30-49 mm

15 x 21.2**
15 x 30 u
/ea
/ea
AJ0-7229
AJ0-7840
AJ0-7839
AJ0-8220
AJ0-7841
AJ0-8162
for ID: 18-29 mm
AJ0-8312
AJ0-8302
AJ0-8301
AJ0-8311
AJ0-8303
AJ0-8309
30-49 mm
SFC
APPROVED
HPLC
10 m
Silica(2)
00G-4091-P0-AX
00G-4091-U0-AX
C5
00D-4092-P0-AX 00G-4092-P0-AX
00G-4092-V0-AX
C8(2)
00B-4250-P0-AX
00G-4250-P0-AX 00B-4250-U0-AX
00G-4250-V0-AX
C18(2)
00B-4253-P0-AX 00D-4253-P0-AX 00G-4253-P0-AX 00B-4253-U0-AX 00G-4253-U0-AX 00B-4253-V0-AX 00G-4253-V0-AX
CN
00G-4300-P0-AX
Phenyl-Hexyl
00G-4285-P0-AX
00G-4285-U0-AX
NH2
00G-4379-P0-AX

15 x 21.2**
15 x 30 u
/ea
/ea
Silica(2)
C5
C8(2)
C18(2)
CN
Phenyl-Hexyl
NH2
SCX
00G-4091-E0
00G-4092-E0
00G-4250-E0
00G-4253-E0
00G-4300-E0
00G-4285-E0
00G-4379-E0
00G-4401-E0
00G-4091-N0
00G-4092-N0
00G-4250-N0
00G-4253-N0
00G-4300-N0
00G-4285-N0
00G-4379-N0
00G-4401-N0

4 x 3.0*
10 x 10
/10 pk
/3 pk
AJ0-4348
AJ0-7223
AJ0-4293
AJ0-7372
AJ0-4290
AJ0-7222
AJ0-4287
AJ0-7221
AJ0-4305
AJ0-7313
AJ0-4351
AJ0-7314
AJ0-4302
AJ0-7364
AJ0-4308
AJ0-7369
for ID: 3.2-8.0 mm
9-16 mm

Luna

Phases
250 x 4.6
250 x 10
15 m Pilot Scale Columns (mm)

Phases
250 x 4.6
Silica(2)
C8(2)
C18(2)
Phenyl-Hexyl
00G-4271-E0
00G-4272-E0
00G-4273-E0
00G-4286-E0
See our latest developments in High-throughout Purifications

starting on page 336
For more dimensions and phases of Axia packed preparative columns,
see pp. 342-343
For Bulk Media, see p. 344
Phenomenex
Method development column kits and method

validation column kits are available. Contact
Phenomenex for details.
Improve analyte sensitivity and reduce baseline
noise with Strata SPE tubes and well plates,
see p. 36 for more information
207
Maxsil
MERCK KGaA, Darmstadt, Germany, Products

The following pages feature a selection of HPLC columns,
packings and accessories. All are designed and manufactured
by Merck KGaA, Darmstadt, Germany, to provide reproducible
results in your critical applications. Reproducibility is guaranteed
because the sorbents, solvents and reagents have been coordinated with one another and have met rigorous standards of
performance.
Merck KGaA HPLC Products

Featured in this Section:
manu-Cart LiChroCART Cartridge Column Assembly
End Fittings for LiChroCART Cartridges
LiChroCART, LiChrospher, LiChrosorb,

Superspher, Purospher
3, 4, 5 and 10 m pre-packed cartridges
Hibar, LiChrosorb and LiChrospher
5 and 10 m pre-packed columns
LiChroCART
MAXSIL / Merck KGaA, Darmstadt, Germany, Products
| HPLC
manu-CART Fittings
LiChroCART Cartridge End-Fittings
Part No.
Mfr. No.
Description
Required End-Fittings For LiChroCART Cartridge Columns
CH0-5286
1.51486.0001 manu-CART end fittings for 2, 3, 4 and 4.6 mm ID LiChroCART HPLC
cartridges stainless steel units for capillary connections 1/16 in. or 0.5 mm OD
LiChrospher
Part No.
Mfr. No.
Description
Stainless Steel Cartridges LiChroCART
CH0-5288 1.50957.0001* LiChrospher 100 RP-18
CH0-5290 1.50823.0001
LiChrospher 100 RP-18
CH0-5291 1.50943.0001
CH0-5293 1.50833.0001
CH0-5294 1.50983.0001
CH0-5299 1.50956.0001* LiChrospher 100 RP-8
CH0-5300 1.50822.0001
CH0-5301 1.50942.0001
CH0-5307 1.50963.0001* LiChrospher 60 RP-select B
CH0-5309 1.50981.0001
LiChrospher 60 RP-select B
CH0-5310 1.50829.0001
CH0-5312 1.50839.0001
CH0-5313 1.50984.0001
CH0-5316 1.50962.0001* LiChrospher 100 RP-18 endcapped
CH0-5317 1.50828.0001
LiChrospher 100 RP-18 endcapped
CH0-5318 1.50734.0001
CH0-5319 1.50838.0001
CH0-5320 1.50995.0001
CH0-5451 1.50960.0001* LiChrospher 100 DIOL
CH0-5329 1.50826.0001
LiChrospher 100 DIOL
CH0-5330 1.50836.0001
LiChrospher 100 DIOL
CH0-5346 1.50834.0001
LiChrospher 100 NH2
*Guard columns
208
Unit
Price
2/pk
Particle Size (m)
Size (mm)
Unit
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4x4
125 x 4
125 x 4
250 x 4
250 x 4
4x4
125 x 4
125 x 4
4x4
125 x 4
125 x 4
250 x 4
250 x 4
4x4
125 x 4
125 x 4
250 x 4
250 x 4
4x4
125 x 4
250 x 4
250 x 4
10/pk
ea
3/pk
ea
3/pk
10/pk
ea
3/pk
10pk
3/pk
ea
ea
3/pk
10/pk
ea
3/pk
ea
3/pk
10/pk
ea
ea
ea
Price
These cartridge columns require

end-fittings. manu-CART end-fittings:
CH0-5286.
Phenomenex

Superspher
Part No.
Mfr. No.
Description
CH0-5368 1.16051.0001
Superspher 100 RP-18
CH0-5369 1.16056.0001
Superspher 100 RP-18
CH0-5371 1.16052.0001
Superspher 60 RP-8
CH0-5372 1.50974.0001
Superspher 60 RP-Select B
CH0-5373 1.50975.0001
CH0-5374 1.50973.0001
CH0-5376 1.16855.0001
Superspher 100 RP-18 endcapped
CH0-5377 1.16858.0001
CH0-5380 1.16857.0001
CH0-5392 1.50197.0001
Particle Size (m)
Size (mm)
Unit
4
4
4
4
4
4
4
4
4
4
125 x 4
250 x 4
125 x 4
75 x 4
125 x 4
250 x 4
125 x 4
250 x 4
250 x 4
125 x 2
ea
ea
ea
3/pk
ea
ea
ea
ea
ea
ea
Particle Size (m)
Size (mm)
Unit
5
5
5
5
5
5
5
4x4
125 x 3
125 x 4
250 x 4
4x4
125 x 4
250 x 4
10/pk
ea
ea
ea
10/pk
ea
ea
Price
Other products available upon request.

These cartridge columns require end-fittings.
manu-CART end-fittings: CH0-5286.
Purospher
Part No.
Mfr. No.
Description
CH0-5422 1.50167.0001*
Purospher RP-18e
CH0-5423 1.50798.0001
Purospher RP-18e
CH0-5425 1.50168.0001
Purospher RP-18e
CH0-5426 1.50169.0001
Purospher RP-18e
CH0-5427 1.50141.0001*
Purospher RP-18
CH0-5428 1.50142.0001
Purospher RP-18
CH0-5429 1.50144.0001
Purospher RP-18
Price
*Guard columns
HPLC | Merck KGaA, Darmstadt, Germany, Products
For Chiral Stationary Phases, see p. 311-326
Hibar Columns
Hibar columns are stainless steel columns that may be connected to virtually any HPLC
instrument using standard 1/16 in. capillary male connection. To prolong the life of the
primary column, we recommend the use of a suitable precolumn (Guard Column).
Columns whose designations include RT are fitted with reducers (R) at both ends;
and are shipped with an individual test chromatogram (T).
LiChrosorb Columns
Part No.
Mfr. No.
Pre-packed Columns Hibar RT
CH0-5396
1.50333.0001
CH0-5397
1.50334.0001
CH0-5398
1.50432.0001
CH0-5399
1.50332.0001
CH0-5400
1.50318.0001
CH0-5408
1.50388.0001
LiChrospher Columns
Part No.
Mfr. No.
Pre-packed Columns Hibar RT
CH0-5409
1.50477.0001
CH0-5410
1.50377.0001
CH0-5414
1.50316.0001
Phenomenex
Description
LiChrosorb RP-18
LiChrosorb RP-18
LiChrosorb RP-8
LiChrosorb RP-8
LiChrosorb RP-8
LiChrosorb Si 60
Description
LiChrospher RP-18
LiChrospher RP-18
LiChrospher Si 100
Particle Size (m)
Size (mm)
Unit
5
10
5
5
10
5
250 x 4
250 x 4
125 x 4
250 x 4
250 x 4
250 x 4
ea
ea
ea
ea
ea
ea
Particle Size (m)
Size (mm)
Unit
5
5
5
125 x 4
250 x 4
250 x 4
ea
ea
ea
Price
Price
209
Nucleosil *
Base deactivated AB, HD and PROTECT I packings

Pore size selection from 50 to 500
Nucleosil is a high surface area spherical silica manufactured by
Macherey-Nagel. This high quality silica has a narrow particle size
distribution making it an efficient packing material for HPLC.
choose the 100 silicas for compounds with MW below 2000 daltons; in general, the 50 and 120 material are suitable for small
molecule analysis. For proteins and other compounds with MW
greater than 2000 daltons, the 300 and larger pore size silicas are
the better choice.
When choosing a Nucleosil column, the compounds molecular

weight (MW) must first be taken into account. Most researchers
3 m 100 Columns (mm)
Phases
50 x 2.0
C18
00B-0319-B0
Nucleosil
| HPLC
5, 7, and 10 m 100 Columns (mm)

Phases
150 x 2.0
150 x 3.2
125 x 4.0
50 x 4.6
100 x 4.6
150 x 4.6
00E-0319-D0
00B-0319-E0
00D-0319-E0
00F-0319-E0
250 x 3.2
125 x 4.0
250 x 4.0
125 x 4.6
150 x 4.6
250 x 4.6

4 x 2.0*
4 x 3.0*
/10pk
/10pk
AJ0-4287
AJ0-4286
for ID: 2.0-3.0 mm
3.2-8.0 mm
250 x 10
5 m Silica
00G-0321-D0
00F-0321-E0 00G-0321-E0
5 m C8
00E-0322-D0 00G-0322-D0
00F-0322-E0 00G-0322-E0
5 m C18
00F-0323-B0 00F-0323-R0 00G-0323-R0 00E-0323-D0 00G-0323-D0 00E-0323-E0 00F-0323-E0 00G-0323-E0 00G-0323-N0
5 m CN
00G-0324-E0
5 m SA
00F-0325-B0
00E-0325-D0
00F-0325-E0 00G-0325-E0
5 m SB
00G-0326-D0
00F-0326-E0 00G-0326-E0
5 m NH2
00G-0327-D0
00G-0327-E0
5 m NO2
00G-0307-E0
7 m Phenyl
00G-0305-E0
10 m C18
00E-0330-D0 00G-0330-D0
00F-0330-E0 00G-0330-E0 00G-0330-N0

10 m CN
00G-0331-D0
00G-0331-E0
4 x 2.0*
4 x 3.0*
10 x 10
/10pk
/10pk
/3pk
AJ0-4347
AJ0-4348
AJ0-7223
AJ0-4289
AJ0-4290
AJ0-7222
AJ0-4286
AJ0-4287
AJ0-7221
AJ0-4304
AJ0-4305
AJ0-7213
AJ0-4307
AJ0-4308
AJ0-7369
AJ0-4310
AJ0-4311
AJ0-7370
AJ0-4301
AJ0-4302
AJ0-7364
AJ0-4350
AJ0-4351
AJ0-7314
AJ0-4286
AJ0-4287
AJ0-7221
AJ0-4304
AJ0-4305
AJ0-7213
for ID: 2.0-3.0 mm

Phases
150 x 3.2
C8
C18
00F-0339-R0
125 x 4.0
100 x 4.6
00E-0339-D0
00D-0335-E0
00D-0339-E0
3.2-8.0 mm
9-16 mm

150 x 4.6
4 x 3.0*
/10pk
00F-0335-E0
AJ0-4290
AJ0-4287
00F-0339-E0
for ID: 3.2-8.0 mm

Phases
250 x 3.2
C18
00G-0341-R0
250 x 4.0
150 x 4.6
250 x 4.6
00G-0341-D0
00F-0341-E0
00G-0341-E0
4 x 3.0*
10 x 10
/10pk
/3pk
AJ0-7221
AJ0-4287
for ID: 3.2-8.0 mm

Phases
250 x 2.0
C18
00G-0277-B0
250 x 4.0
150 x 4.6
250 x 4.6
00G-0277-D0
00F-0277-E0
00G-0277-E0
9-16 mm

4 x 2.0*
4 x 3.0*
/10pk
/10pk
AJ0-4320
AJ0-4321
for ID: 2.0-3.0 mm
3.2-8.0 mm
100 HD, AB, Protect I

Part No.
Mfr. No.
CH0-4752
720193.46
CH0-4753
720192.46
CH0-4756
720294.46
CH0-4757
720280.46
CH0-4759
720936.46
Description
3 m C18 HD
3 m C18 HD
5 m C18 HD
5 m C18 HD
5 m C18 AB
Size (mm)
150 x 4.6
250 x 4.6
150 x 4.6
250 x 4.6
250 x 4.6
Price
For Chiral Stationary Phases, see p. 324

*Nucleosil is a registered trademark of Macherey-Nagel
210
Phenomenex
Onyx
If Onyx analytical columns do not provide

compared to a competing column of the
same monolithic characteristics, similar
phase, and dimensions, return the
column with comparative data within
Finish First with Monolithic

Silica HPLC Columns
Onyx is a silica monolithic HPLC column designed for high speed
analysis. The monolithic nature allows for "dilute-and-shoot" applications saving scientists valuable sample preparation time.
Reduce run times by more than 50 %
Dilute-and-Shoot dirty biological samples
Analytical, capillary, and semi-prep dimensions
Packing Material
Macropore
Size
(m)
Mesopore
Size
()
Pore
Volume
(mL/g)
Surface
Area
(m2/g)
Carbon
Load
%
2
2
2
1.5
1
130
130
130
130
130
1.0
1.0
1.0
1.0
1.0
300
300
300
300
300
0
11
18
18
18
Onyx Silica
Onyx C8
Onyx C18
Onyx C18*
Onyx C18-HD
Calculated Bonded
End
Phase Coverage
Capping
(mole/m2)
0
3.8
3.6
3.6
3.6
No
Yes
Yes
Yes
Yes
Maximum Pressure: 200 Bar; pH Range: 2.0-7.5

*50 x 2.0 mm ID only; enhanced 1.5 m macropre size for higher efficiencies
HPLC | Onyx
Monolithic Technology vs. Particle-Based Technology

Onyx:
Particle-Based Columns:
Monolithic porous silica rod
Individual silica particles
Significantly shorter run times

Cut methods by more than half
High flow resistance

Limits ability to shorten run times
Low backpressures
Less stress on system and
column
Increased backpressure
Limits life of pumps, seals, and column
High flow rates
Reduced throughput
Long run times
Due to high porosity

No inlet bed settling
Increased reliability,
reproducibility, and lifetime
Phenomenex
Bed splitting possible

Shortens column life & lessens
reproducibility
211
Onyx
Dramatically Increase Throughput

and Reduce Analysis Time
Onyx columns can be used in a variety of reversed phase methods - anytime you want the advantage of speed and throughput,
put Onyx to the test!
Option to run from 1 mL/min up to 9 mL/min

Reduce re-equilibration time from sample to sample
Shorten total separation time once target compound
has eluted with flow gradient options
You can also scale existing Onyx methods to the new 50 x 2.0
mm ID to gain advantages in sensitivity and solvent savings.
Versatile Performance!
Antihistamines
Onyx Monolithic C18 50 x 4.6 mm
0.5
100
mAU
80
100
60
80
40
60
20
40
0
20
0
6
1.5
min
1.5
min
Onyx Monolithic C18 50 x 2.0 mm

Over 5x reduction
in solvent usage!
1
1
6
6
0.5
1.5
min
0.5
1.5
min
Extremely Low Backpressure
High Efficiencies
The very high porosity of Onyx columns result in very low backpressures, even at high flow rates. Onyx silica monolithic columns
rarely exceed 100 bar, even at 9 mL/min, while particle-based
columns reach backpressure limits at much lower flow rates.
Onyx 2.0 mm ID columns have a reduced macropore of 1.5 m,

providing excellent effiiciencies.
Typically 60 % less backpressure than particle-based

columns
22
Couple columns together to produce extremely high

plate counts to separate critical pairs
Minimal worry of system shutdowns from high
backpressure
20
18
Plate Height (m)
Onyx | HPLC
0.5
mAU
50
0
0
App ID 16670
Conditions same for both separations:

Mobile Phase: A: 0.1 % Phosphoric Acid in Water
B: 0.1 % Phosphoric Acid in Acetonitrile
Gradient: 10 % to 50 % B in 3.4 min
Flow Rate: 0.56 mL/min for 2.0 mm ID
3.0 mL/min for 4.6 mm ID
Sample: 1. Pyrilamine
2. Tripelennamine
3. Chlorpheniramine
4. Brompheniramine
5. Chloropyramine
6. Diphenhydramine
App ID 16671
mAU
350
300
mAU
250
350
200
300
150
250
100
200
50
150
0
100
16
16
Over 100,000
plates per meter at fast
flow rates!
12
Backpressure vs. Flow Rate
10
250
3 m Particle-Based column
Backpressure (bar)
200
5 m Particle-Based column
150
100
Onyx
50
0
0
Flow Rate (mL/min)
212
Particle-based 5 m Column 50 x 2.0 mm

Onyx Monolithic Column 50 x 2.0 mm
Linear Velocity (mm/sec)

Columns: Particle-based 5 m Column 50 x 2.0 mm ID
Onyx Monolithic Column 50 x 2.0 mm ID
Mobile Phase: Acetonitrile/Water (65:35)
Flow Rate: As noted
Temperature: 30 C
Samples: 1. Uracil
2. Acetophenone
3. Benzene
4. Toluene
5. Naphthalene
Phenomenex
Onyx
Faster Throughput for Bioanalytical Samples

In DMPK/ADME and clinical environments, polar drugs and metabolites must be separated from complex matrices. This often
involves rigorous sample cleanup procedures prior to injection onto
the HPLC.
backpressure no longer a concern, gradient cycle times can be decreased by increasing flow rate during the hold and re-equilibration
step, significantly improving the speed of sample throughput.
In addition, aggressive gradient conditions often employed require

lengthy column re-equilibration times between injections. With
Over 40 hours saved!
Compared to a traditional re-equilibration strategy at 0.6 mL/min for 1.0 min
2nd Injection
3
2
App ID 16638
1.0
Fast Cycle Times!
min
1 mL/min
0.6 mL/min
5,000 Injection
th
3
2
1
HPLC | Onyx
App ID 16637

Column: Onyx Monolithic C18
Part No.: CH0-8373
Gradient: 5 % B to 95 % B in 1.5 min at 0.6 mL/min
Equilibrate: 5 % B for 0.5 min at 1.0 mL/min
Temperature: 45 C
Sample: (100 g/mL) in 1:3 Human plasma: Acetonitrile
1. Atenolol
2. Pindolol
3. Metoprolol
4. Alprenolol
min
1.0
Food and Beverage:

Flow restrictions and overpressures, due to salts,
precipitated proteins, and lipids in the sample matrix, are
highly unlikely
Increase resolving power of very complex food extracts by
column coupling
Analyze very dilute or low-level analytes by a direct, high-flow
injection onto the column
App ID 15589
Multi-Grain Cereal
Phenomenex
Column: Onyx Monolithic C18

(2 x 100 x 4.6 mm columns coupled in series)
Part No.: CH0-7643
Mobile Phase: A: 0.1 % TFA in Water
B: 0.08 % TFA in Acetonitrile
Gradient: 5-70 % B in 15 minutes
Col. Temperature: 30 C
Sample: Multi-grain cereal
min
213
If Onyx analytical columns do not provide at

least an equivalent separation as compared to
a competing column of the same monolithic
characteristics, similar phase, and dimensions,
return the column with comparative data within
Onyx
10 mm ID Onyx Semi-Prep Column

Flow rates from 5 35 mL/min
Loading capacities approaching what is typically
observed on 21.2 mm ID columns for some samples
n High Resolution Monolithic Columns

e
w Onyx HD-C18
Pore structure rapidly disrupts DMSO injection slug

resulting in better mixing & improved binding of analyte
to sorbent
50 % higher performance compared to our standard Onyx

columns
Backpressure 2 times lower than particle packed columns
30 % longer column lifetime compared to some particle
packed columns
Long lifetimes when analyzing dirty samples due

to monolithic nature
Excellent Reproducibility
Refer to technical note, TN-1025, for more information pertaining

to Onyx reproducibility. Call your Phenomenex representative.
Onyx | HPLC
Several parameters, such as peak asymmetry and retention factors, were used to test the reproducibility of Onyx silica monolithic
columns and ensure that every batch meets the quality control
standards of chromatographers worldwide.
Part No.
Description
Capillary Columns
CH0-8388
Onyx Monolithic C18
CH0-7646
Onyx Monolithic C18
CH0-8389
Onyx Monolithic HD-C18
CH0-8390
Onyx Monolithic C18
CH0-8391
CH0-8392
Onyx Monolithic C18 Trapping Column
CH0-8393
Onyx Monolithic C8
Analytical Columns
CH0-8373
Onyx Monolithic C18
CH0-8467
Onyx Monolithic C18
CH0-8464
Onyx Monolithic C18
CH0-8463
Onyx Monolithic C18
CH0-8158
Onyx Monolithic C18
CH0-7643
Onyx Monolithic C18
CH0-7644
Onyx Monolithic C18
CH0-7645
Onyx Monolithic C18
CH0-8611
CH0-8612
CH0-8613
CH0-7647
Onyx Monolithic C8
CH0-7648
Onyx Monolithic Si
SemiPrep Columns
CH0-7878
Onyx Monolithic C18
Guard Cartridge System
KJ0-8468
Onyx Monolithic C18 Guard Cartridge Kit (3 pk cartridges + holder + wrench)
CH0-8469
Onyx Monolithic C18 Guard Cartridges (3/pk)
KJ0-8465
CH0-8466
KJ0-7651
CH0-7649
KJ0-8615
Onyx Monolithic HD-C18 Guard Cartridge Kit
(3 pk cartridges + holder + wrench)
CH0-8616
Onyx Monolithic HD-C18 Guard Cartridge (3/pk)
KJ0-7652
CH0-7650
Method Validation Kit
KH0-7653
Onyx Monolithic C18 Method Validation Kit (3 columns from different batches)
Column Coupler
AQ0-7654
Onyx Column Coupler
Size (mm)
Price
150 x 0.05
150 x 0.1
150 x 0.1
150 x 0.2
150 x 0.2
50 x 0.2
150 x 0.1
50 x 2.0
100 x 2.0
25 x 3.0
50 x 3.0
100 x 3.0
100 x 4.6
50 x 4.6
25 x 4.6
100 x 4.6
50 x 4.6
25 x 4.6
100 x 4.6
100 x 4.6
100 x 10.0
5 x 2.0
5 x 2.0
5 x 3.0
5 x 3.0
5 x 4.6
5 x 4.6
5 x 4.6
5 x 4.6
10 x 4.6
10 x 4.6
For Fused Silica Capillary Adapter, see

p. 360
100 x 4.6
For Onyx Normal and Reversed Phase

Column Check Standards, see p. 365
Product based on monolithic

technology under license from Merck
KGaA, Darmstadt, Germany
214
Phenomenex
Partisil *
Quality-packed by Phenomenex
Irregular silica-based materials manufactured by Whatman
Packing Material
Silica
C8
ODS(1)
ODS(2)
ODS(3)
PAC
SAX
SCX
Particle
Shape/Size
(m)
Pore
Size
()
Surface
Area
(m2/g)
Carbon
Load
%
Calculated Bonded
Phase Coverage
(mole/m2)
End
Capping
Irreg. 5, 10
Irreg. 5, 10
Irreg. 10
Irreg. 10
Irreg. 5, 10
Irreg. 5, 10
Irreg. 10
Irreg. 10
85
85
85
85
85
85
85
85
350
350
350
350
350
350
350
350
0
8.5
5
16
10.5
0.85(N)
0.85(NR+3)
0.40(S)
0
2.33
0.63
2.43
1.45
No
Yes
No
No
Yes
No
No
No
Sympathomimetic Drugs
Column:
Dimensions:
Part No.:
Mobile Phase:
5 m Columns (mm)
Phases
Silica
C8
ODS (3)
PAC
4
5
App ID 5426
2
1
12
16
20
24 min
100 x 4.6
150 x 4.6
250 x 4.6
00D-0119-E0
00D-0120-E0
00F-0119-E0
00F-0120-E0
00G-0118-E0
00G-0120-E0
00G-0121-E0

4 x 3.0
/10pk
AJ0-4348
AJ0-4290
AJ0-4287
HPLC | pARTISIL
Partisil 5 m ODS(3)
250 x 4.6 mm
00G-0120-E0
0.01 M Octanesulfonic acid in 0.2 %
Glacial acetic acid/Methanol (70:30)
Flow Rate: 1 mL/min
Sample: 1. Epinephrine
2. Levonordefrin
3. Isoproterenol
4. Phenylephrine
5. Metaraminol
for ID: 3.2-8.0 mm
10 m Columns (mm)
Phases
250 x 4.6
Silica
C8
ODS (1)
ODS (2)
ODS (3)
SCX
SAX
00G-0122-E0
00G-0123-E0
00G-0124-E0
00G-0125-E0
00G-0126-E0
00G-0127-E0
00G-0128-E0
SecurityGuard
Cartridges (mm)
4 x 3.0
/10pk
AJ0-4348
AJ0-4290
AJ0-4287
AJ0-4287
AJ0-4287
AJ0-4308
AJ0-4311
for ID: 3.2-8.0 mm
*Partisil is a registered trademark of Whatman.
Phenomenex
215
Phenogel GPC/SEC Columns
Organic Size Exclusion/Gel Permeation

for Polymer Analysis
5 and 10 m particle sizes
Narrow bore (4.6 mm ID) solvent-saver to preparative
columns available
Technical Specifications
Material:
Particle Size:
Porosities:
Typical Pressure:
Very good alternative to Polymer Labs PLgel and

Waters Styragel, Ultrastyragel, Styragel HT, and
Styragel HR columns
Highly cross-linked for mechanical and
chemical stability
Maximum Pressure:
Maximum Temperature:
Minimum Efficiency*:
Temperature stable to 140 C

Phenogel is available in seven different pore sizes ranging from 50
to 106 , and a linear bed configuration. Pore size distribution and
pore volume are closely controlled parameters in the manufacturing
process; accounting for the high resolution, tight linear calibration
curves and excellent column-to-column reproducibility.
Typical Flow Rates:
End Fittings:
* Tested in THF
Sample Elution
SDVB
5, 10 m
50 to 106 , and mixed beds
5 m: 350 psi
10 m: 200 psi
650 psi
140 C
5 m: 45,000 p/m**
10 m: 35,000 p/m**
4.6 mm ID: 0.35 mL/min
Valco Compatible
** For 300 x 7.8 mm ID columns
See note on p. 389 regarding pore sizes and exclusion limits
Each standard dimension Phenogel column (300 x 7.8 mm) has an

internal volume of 15 mL that is distributed as follows:
3 mL is occupied by the solid portions of the gel particles
(20 % of total column volume)
6 mL is the interstitial volume or volume between the gel particles

Thus, about 6 mL of solvent must elute through each column before
even the largest molecules can emerge, while the smallest molecules emerge with the total column volume of 12 mL. This constant
distribution of volume makes it possible to predict the amount of
solvent and time necessary to complete any analysis.
SEM Photos of Phenogel Polymer Beads
103
106
Column Selection by Molecular Weight

Sample Type
Small Organics
Resins
High MW Polymers
Molecular
Weight
Phenogel
Column
100 - 3 K
500 - 6 K
1 K - 15 K
1 K - 75 K
5 K - 500 K
10 K - 1,000 K
60 K - 10,000 K
100 - 10,000 K
50
100
500
103
104
105
106
Linear(2)
Column Molecular Weight Calibration Curves
Polystyrene Molecular Weight
phenogel Gpc/sec columns | HPLC
6 mL is the pore volume of the packing material

Elution Volume/Column (mL)
216
Phenomenex
Solvent and Temperature Compatibility
Solvent Switching Considerations
Phenogel columns are packed in tetrahydrofuran (THF)

Columns can also be shipped in solvents such as DMF,
Methylene Chloride, NMP, and o-CP, to help minimize
equilibration time
Although Phenogel columns are rugged and can withstand strong

solvent changes, care should be exercised when switching from
high-swell solvents (A) to low-swell solvents (B, C, and D). Improper
solvent switches can result in a void. Best results are attained when
an intermediate-swell solvent is used, and column lifetime is improved. Contact Phenomenex regarding solvents not listed below.
Solvent Compatibility Table
Column life can be maximized by dedicating certain columns to

certain solvents. This will also minimize solvent switches. If care is
not taken, a void may occur.
Phenogel Pore Size:
N = Not Compatible
Y = Compatible
Phenomenex
103
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
N
Y
104
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
N
Y
105
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
N
Y
106
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
N
Y
Linear &
Mixed
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
N
Y
Suggested
Operating
Temp.
Reduce flow rate to 0.2 mL/min

Backpressure must NEVER exceed 650 psi
Always check solvent miscibility in a beaker or follow
the solvent miscibility table on page 387 before proceeding
with ANY solvent switch.
60 C
60 C
Compare the swell characteristics of solvent 1 (old solvent)

to solvent 2 (new solvent) and use the following guidelines:
60 C
If solvent 1 and solvent 2 belong to the same swell category (see table
below), check the solvent miscibility and proceed with the switch.
100 C
100 C
135 C
60 C
135 C
If solvent 1 and solvent 2 belong to successive swell categories as

indicated by the arrows in the table below, check the miscibility and
proceed with the switch.
If solvent 1 and solvent 2 DO NOT belong to the same OR successive swell
categories, switch to an intermediate solvent FIRST, as indicated by the
arrows in the table.
HPLC | phenogel Gpc/sec columns
()
Mobile Phase Solvent
50
100 500
Acetone
Y
Y
Y
Benzene
Y
Y
Y
Carbon Tetrachloride
Y
Y
Y
Chloroform
Y
Y
Y
30 % HFIP/Chloroform
Y
Y
Y
Diethyl Ether
Y
Y
Y
Dimethylacetamide (DMAC)
Y*
Y
Y
Dimethylformamide (DMF)
Y*
Y
Y
Dioxane
Y
Y
Y
DMSO
Y*
Y
Y
Ethyl Acetate
Y
Y
Y
Hexafluoroisopropanol (HFIP)
Y
Y
Y
Hexane
Y
Y
Y
M-Cresol
Y*
Y
Y
Methyl Ethyl Ketone
Y
Y
Y
Methylene Chloride
Y
Y
Y
0-Chlorophenol
Y*
Y
Y
0-Dichlorobenzene
Y*
Y
Y
Quinolin
Y*
Y
Y
Tetrahydrofuran
Y
Y
Y
Toluene
Y
Y
Y
Trichlorobenzene
Y*
Y
Y
Water
N
N
N
Xylene
Y
Y
Y
*Not recommended on 5 m 50 columns.
217
50 - 106 Columns
Closely Related Hydrocarbons

Column:
Dimensions:
Solvent:
Flow Rate:
Detection:
Injection Volume:
Temperature:
Sample:
High resolution at low cost

Customize your analysis by coupling
different pore-size columns
Wide range of solvent compatibility
Polymethyl Methacrylates (Wide MW Range)
App ID 5432
Phenogel 5 m 105 , 104 , 103 , 500

300 x 7.8 mm
THF
1.0 mL/min
Differential Refractometer
100 L 0.25 % w/v
1. 700,000 MW
2. 107,000 MW
3. 27,000 MW
App ID 5431
Column:
Dimensions:
Solvent:
Flow Rate:
Detection:
Injection Volume:
Sample:
Phenogel 5 m 50 , 100 , 500

300 x 7.8 mm
THF
1.0 mL/min
100 L 0.25 % w/v
Ambient
1. C40 562 MW 4. C20 282 MW
2. C32 450 MW 5. C16 226 MW
3. C24 338 MW 6. C13 184 MW
Column:
Dimensions:
Solvent:
Flow Rate:
Detection:
Injection Volume:
Temperature:
Sample:
Phenolic Resins
Phenogel 5 m 50 , 100 , 500

300 x 7.8 mm
THF
1.0 mL/min
100 L 0.25 % w/v
Ambient
1. dp7 546 MW 5. dp3 194 MW
2. dp6 458 MW 6. dp2 106 MW
3. dp5 370 MW 7. dp1 62 MW
4. dp4 282 MW
Column:
Dimensions:
Solvent:
Flow Rate:
Detection:
Injection Volume:
Temperature:
Sample:
Phenogel 5 m 500 x 2, 103 , 104

300 x 7.8 mm
THF
1.0 mL/min
RI
5 L
25 C
Phenolic Aldehyde Resin, MW 500 to 470 K
Refrac Chromatogram
14.00
4
5
12.00
App ID 5429
3
10.00
8.00
6.00
1
App ID 5433
Rel Inten X10-1
Polyethylene Glycol 330
4.00
2.00
0.00
20.00
21.00
22.00
23.00
24.00
25.00
26.00
27.00
Ret V ol (mL)
Polyvinyl Butyral
Column:
Dimensions:
Solvent:
Flow Rate:
Detection:
Injection Volume:
Temperature:
Sample:
Phenogel 10 m 100
250 x 21.2 mm
00G-0642-P0
Dichloromethane
4.0 mL/min
UV @ 254 nm
Ambient
1. Diethylhexyl Phthalate
2. Biphenyl
3. Naphthalene
4. Coronene
5. Sulfur
App ID 5430
Column:
Dimensions:
Part No:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
218
Phenogel 5 m 500, 103, 104, 105

300 x 7.8 mm
THF
1.0 mL/min
100 L 0.25 % w/v
25 C
300,000 MW
App ID 5438
Organic Compounds
Phenomenex
50 - 106 Columns (cont'd)

Polyethylene Oxide (PEO)
Polystyrenes (Wide MW Range)

Column:
Dimensions:
Mobile Phase:
Flow Rate:
Detection:
Injection Volume:
Temperature:
Sample:
Phenogel 10 m 105, 104, 103

300 x 7.8 mm
DMF (0.1 M LiBr)
1.0 mL/min
100 L 0.125 % w/v
50 C
400,000 MW
Phenogel 10 m 105, 104, 103

300 x 7.8 mm
THF
1.0 mL/min
100 L 0.125 % w/v
Ambient
1. 1,560,000 MW 5. 6,100 MW
2. 260,000 MW 6. 845 MW
3. 94,000 MW 7. 146 MW
4. 30,000 MW
App ID 5435
App ID 5434
Polybutadines (Wide MW Range)

Column:
Dimensions:
Solvent:
Flow Rate:
Detection:
Injection Volume:
Sample:
Phenogel 5 m 50 , 100 in series

300 x 7.8 mm
THF
0.25 mL/min
1. Squalene C30
(2, 6, 10, 15, 19, 23-Hexamethyltetracosane)
2. Phytane C20 (2, 6, 10, 14-Tetramethylhexadane)
3. Farnesne C15 (2, 6, 10-Trimethyldodecane)
Poly-(-Methyl Styrene) (Wide MW Range)

App ID 5436
45
Phenogel 5 m 105, 104, 103, 500

300 x 7.8 mm
THF
1.0 mL/min
100 L 0.25 % w/v
1. 420,000 MW
2. 24,000 MW
3. 2,500 MW
4. 500
MW
50
55
Column:
Dimensions:
Solvent:
Flow Rate:
Detection:
Injection Volume:
Sample:
min
The columns were used in tandem to characterize isoprene chain lengths removed from labeled in vitro
translation products or cell proteins. The isoprenoids were removed by treatment with Raney nickel
and extracted into pentane. The pentane extractable material was hydrogenated over platinum catalyst
and injected onto the column. Fractions were collected at 0.5 minute intervals and radioactivity was
monitored by liquid scintillation. These saturated hydrocarbon chains were characterized by comparing
radioactive peaks to standard retention times.
Phenogel 5 m 105, 104 , 103 , 500

300 x 7.8 mm
THF
1.0 mL/min
100 L 0.25 % w/v
1. 680,000 MW
1
2. 90,000 MW
3. 30,000 MW
2
4. 6,000 MW
3
Chromatogram courtesy of W. Maltese and R. Erdman, Weis Center for Research, Geisinger Clinic.
Phenomenex
Column:
Dimensions:
Mobile Phase:
Flow Rate:
Detection:
Sample:
App ID 5437
Isoprenes from In Vitro Translation on Products
10
20
30
App ID 5439
Column:
Dimensions:
Mobile Phase:
Flow Rate:
Detection:
Injection Volume:
Temperature:
Sample:
40
min
219
Linear Columns
Linear calibration to 10 million daltons
Long column life
Excellent mechanical stability
Excellent for analyzing a wide range
of molecular weights
Cyclic Polymer Characterization
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Injection Volume:
Temperature:
Sample:
Phenogel 10 m Linear(2)
600 x 7.8 mm
00K-3260-K0
THF with 1 % TEA
1.3 mL/min
UV @ 268 nm
40 L 0.2 % w/v
Ambient
Poly-(2-Vinylpyridine) [0.05 % - 0.25 % (w/v)]
Calibration Curve: Linear (2) - Phenogel 5 m 300 x 7.8 mm

108
107
106
MW
105
Crude
104
y = 4.47e + 11
App ID 5441
103
Fractionated
10
11
12
13
14
15
16
17
R = 0.99
102
101
4
10
11 min
18
Elution Vol (mL)
Narrow Bore Columns

An Improved Dimension in
GPC Analysis
Decrease solvent consumption
Retain same elution profile
Reduce solvent disposal costs
Phenogel-NB (NarrowBore) columns are optimized to reduce
solvent consumption. The Phenogel-NB columns have a 4.6 mm
column ID and run at 0.35 mL/min, reducing solvent consumption
and disposal costs up to 65 %!
Loading
With narrow bore GPC/SEC columns, the volume in which the
sample elutes is significantly decreased, thus increasing the effective concentration of the sample. This increase in sensitivity is
exploited in HPLC, but in GPC it leads to overloading effects and
proportionally lower sample loadings must be used.
103
220
104
105
106
107
Phenomenex
If Phenogel analytical columns do not

provide at least equivalent separation
as compared to a competing column
of the similar particle size, phase, and
FULL REFUND.
5 m Columns (mm)
Pore Size
50
100
500
103
104
105
106
MW Range
100-3 K
500-6 K
1K-15 K
1K-75 K
5K-500 K
10K-1,000 K
60K-10,000 K
Mixed Beds
Linear(2)
100-10,000 K
300 x 7.8
600 x 7.8
Guards
50 x 7.8
00H-0441-K0
00H-0442-K0
00H-0443-K0
00H-0444-K0
00H-0445-K0
00H-0446-K0
00H-0447-K0
300 x 7.8
00K-0442-K0
00K-0445-K0
00K-0446-K0
600 x 7.8
03B-2088-K0
03B-2088-K0
03B-2088-K0
03B-2088-K0
03B-2088-K0
03B-2088-K0
03B-2088-K0
50 x 7.8
Other Shipping Solvents:

Methanol, Methylene Chloride, Cyclohexane, Ethyl
Acetate, NMP, DMAC, DMF
Size (mm)
Price
30 x 4.6
47
50 x 4.6
52
300 x 4.6
62
300 x 7.8
73
600 x 7.8
78
00H-3259-K0
00K-3259-K0
03B-2088-K0
NOTE: Phenogel columns are routinely shipped in THF.

Columns can be shipped in Toluene and Chloroform upon
request at no additional charge.
5 m Narrow Bore (NB) Columns (mm)

Pore Size
50
100
500
103
104
MW Range
100-3 K
500-6 K
1K-15 K
1K-75 K
5K-500 K
300 x 4.6
Guards
30 x 4.6
00H-0441-E0
00H-0442-E0
00H-0443-E0
00H-0444-E0
00H-0445-E0
03A-2088-E0
03A-2088-E0
03A-2088-E0
03A-2088-E0
03A-2088-E0
Pore Size
50
100
500
103
104
105
106
MW Range
100-3 K
500-6 K
1K-15 K
1K-75 K
5K-500 K
10K-1,000 K
60K-10,000 K
Mixed Beds
Linear(2)
100-10,000 K
300 x 7.8
600 x 7.8
Guards
50 x 7.8
00H-0641-K0
00H-0642-K0
00H-0643-K0
00H-0644-K0
00H-0645-K0
00H-0646-K0
00H-0647-K0
300 x 7.8
00K-0641-K0
00K-0642-K0
00K-0643-K0
00K-0644-K0
00K-0645-K0
00K-0646-K0
00K-0647-K0
600 x 7.8
03B-2090-K0
03B-2090-K0
03B-2090-K0
03B-2090-K0
03B-2090-K0
03B-2090-K0
03B-2090-K0
50 x 7.8
00H-3260-K0
00K-3260-K0
03B-2090-K0
For full line of Polymer Calibration

Standards, see p. 367
Phenomenex
Manufacturer
Jordi Associates
Polymer Labs
Waters
Columns
Jordi GPC-DVB
PLgel
Styragel
Styragel
Ultrastyragel
Styragel HT
Styragel HR
10 m Columns (mm)
Phenogel Columns are a

Recommended Alternative to:
All other column dimensions available. Phenogel columns are

routinely shipped in THF. However, columns are also available in
commonly used solvents such as Toluene and Chloroform as well
as DMF, NMP, and other solvents. Refer to the chart above for
the additional charge for these shipping solvents. Please specify
shipping solvent when ordering.
221
PhenoSphere
Cost-effective, HPLC columns
Excellent Alternative to:
NEXT generation features more rugged C18, C8, CN

and Phenyl phases for the same low price. See below.
IB-Sil
PrimeSphere
Selectosil
Packing Material
C1
C6
C8
ODS (1)
ODS (2)
CN (1)
NH2
SCX
SAX
Particle
Shape/Size
(m)
Pore
Size
()
Pore
Volume
(mL/g)
Surface
Area
(m2/g)
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5
Spher. 3, 5, 10
Spher. 5, 10
Spher. 5, 10
80
80
80
80
80
80
80
80
80
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
220
220
220
220
220
220
220
220
220
00F-3222-R0
00F-3169-R0
phenosphere / phenosphere - Next | HPLC
50 x 4.6
150 x 4.6
00B-4084-E0
00F-3222-E0
00F-3169-E0
00F-3170-E0
00F-4084-E0
1.08
2.27
3.54
1.74
2.50
2.82
1.58
0.4meq/g
0.6meq/g
No
Yes
Yes
Partial
Yes
Partial
No
No
No
00F-3151-B0

Cartridges, see p. 235

4 x 2.0
4 x 3.0
/10pk
/10pk
AJ0-4286
AJ0-4287
AJ0-4286
AJ0-4287
AJ0-4304
AJ0-4305
AJ0-4301
AJ0-4302
3.2-8.0 mm
150 x 3.2
250 x 3.2
150 x 4.6
250 x 4.6
00F-3172-R0
00F-3151-R0
00G-3172-R0
00G-3151-R0
00F-4085-E0
00F-4086-E0
00F-3149-E0
00F-3172-E0
00F-3174-E0
00F-4087-E0
00F-3151-E0
00F-3223-E0
00G-4085-E0
00G-4086-E0
00G-3149-E0
00G-3148-E0
00G-3172-E0
00G-3174-E0
00G-4087-E0
00G-3151-E0
00G-3223-E0
PhenoSphere - NEXT

4 x 2.0
4 x 3.0
/10pk
/10pk
AJ0-4298
AJ0-4299
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
AJ0-4286
AJ0-4287
AJ0-4304
AJ0-4305
AJ0-4301
AJ0-4302
AJ0-4310
AJ0-4311
AJ0-4307
AJ0-4308
for ID: 2.0-3.0 mm
3.2-8.0 mm
Packing Material
Silica
C8
C18
CN
Phenyl
Particle Shape/
Size
(m)
Pore
Size
()
Surface
Area
(m2/g)
Carbon
Load
%
End
Capping
Spher. 3, 5
Spher. 3, 5
Spher. 3, 5
Spher. 3, 5
Spher. 5
120
120
120
120
120
380
380
380
380
380
10
14
8
11
No
Yes
Yes
Yes
Yes
SecurityGuard Analytical Cartridges require universal holder Part No.: KJ0-4282
5 m LC/MS Columns (mm)

Phases
50 x 2.0
C18
Phenyl
CN
Silica
C8
C18
CN
Phenyl
150 x 2.0
00B-4309-B0
5 m Columns (mm)
Phases
150 x 2.0
222
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
Monomeric
End
Capping
for ID: 2.0-3.0 mm
5 m Columns (mm)
Phases
150 x 2.0
C1
C6
C8
ODS (1)
ODS (2)
CN (1)
NH2
SAX
SCX
4,
6,
6,
7,
11,
4,
2,
6,
4,
Calculated Bonded
Phase Coverage
(mole/m2)
SecurityGuard Analytical Cartridges require holder, Part No.: KJ0-4282
3 m Columns (mm)
Phases
150 x 3.2
ODS (1)
ODS (2)
CN (1)
NH2
Carbon
Load
%
00F-4308-B0
00F-4308-B0

4 x 2.0
4 x 3.0
/10pk
/10pk
AJ0-4286
AJ0-4287
AJ0-4350
AJ0-4351
AJ0-4304
AJ0-4305
for ID: 2.0-3.0 mm
3.2-8.0 mm
150 x 3.2
125 x 4.0
150 x 4.6
250 x 4.6
00F-4308-R0
00E-4307-D0
00E-4308-D0
00F-4306-E0
00F-4307-E0
00F-4308-E0
00F-4310-E0
00G-4306-E0
00G-4307-E0
00G-4308-E0
00G-4310-E0
00G-4309-E0

4 x 2.0
4 x 3.0
/10pk
/10pk
AJ0-4347
AJ0-4348
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
AJ0-4304
AJ0-4305
AJ0-4350
AJ0-4351
for ID: 2.0-3.0 mm
3.2-8.0 mm
Phenomenex
Polymer Labs, PLgel , PLRP-S
Available from Phenomenex in North America only.
PLgel GPC Columns

PLgel is a highly cross-linked spherical polystyrene/divinylbenzene matrix for non-interactive GPC/SEC of polymers.
Use Phenogel GPC Columns as an

Alternative to PLgel
See p. 216
3 m Mixed Columns (mm)
Phase
300 x 7.5
Inquire
1110-6300
3 m Mixed-E (30K)
5 m Mixed Columns (mm)

Phases
MW Range
5 m Mixed-D
5 mmMixed-C
Mixed-C
200-400 K
200-3000 K
5 m Linear Columns (mm)

Pore Size ()
MW Range
50
100
10E3
10E4
10E5
< 1000
< 4000
1 K - 40 K
4 K - 400 K
40 K - 4000 K
300 x 7.5
Inquire
1110-6504
1110-6500
Guards
50 x 7.5
Inquire
1110-1520
1110-1520
300 x 7.5
Inquire
1110-6515
1110-6520
1110-6530
1110-6540
1110-6550
Guards
50 x 7.5
Inquire
1110-1520
1110-1520
1110-1520
1110-1520
1110-1520
The PLRP-S adsorbent is a spherical, rigid, macroporous

polystyrene/divinylbenzene HPLC packing. The homogeneous
aromatic structure and high surface area create an extremely
hydrophobic packing with a pH range of 1 to 13.
PLRP-S Columns
Description
PLRP-S Guard Col (2pk)
PLRP-S 100 5 m
PLRP-S 100 5 m
PLRP-S 100 5 m
PLRP-S 100 8 m
Size (mm)
50 x 3.0
50 x 4.6
150 x 4.6
250 x 4.6
250 x 4.6
Part No.
CH0-0652
CH0-0653
CH0-0654
CH0-0655
CH0-0657
Mfr. No.
1612-1801
1512-1500
1111-3500
1512-5500
1512-5800
Use PolymerX RP-1 Columns as an

Economical Alternative to PLRP-S
See p. 224
HPLC | Polymer Labs, Plgel, plrp-s
PLRP-S Columns
PricePrice
Inquire
Inquire
Inquire
Inquire
Inquire
For a full line of Polymer Calibration Standards, see p. 367
Phenomenex
223
PolymerX
If PolymerX analytical columns do not

provide at least equivalent separation
as compared to your PSDVB column
of similar particle size and dimension,
return the column with comparative
Reversed Phase Polymer

HPLC Columns
Excellent alternative to other polystyrene
divinylbenzene (PSDVB) columns
High chemical strength and stability

pH stable from 0-14
No bonded phase = zero phase bleed
Packing Material
Particle Shape/Size (m)
Pore Size ()
pH Stability
RP-1 (PSDVB)
Spherical 3, 5, 7, 10, 15
100
0 - 14
Typical Results and Operating Parameters of RP Silica

and Polymer Columns
Great for small proteins, peptides and

biological molecules
Parameter
C18 silica
RP-polymer
Acidic silanols
present
absent
pH stability
2-9
0-14
Recovery*
~50-80 %
>95 %
Capacity*
1 mg
10-25 mg
Pressure limit
3500 psi
2500 psi
Temperature limit
60 C
60 C
Column lifetime
longer
*pertains to dimethyltritylated (DMT) synthetic oligomer purification on a 150 x 4.1 mm
column
PolymerX RP-1 consists of microporous (100 ) polystyrene

divinylbenzene (PSDVB), which is inherently similar to reversedphase chemistries, displaying similar selectivity characteristics as
C18-bonded silica columns. Because bare PSDVB shows similar
selectivity to a C18 column, no surface ligands are bonded to the
polymer. In turn, this means no phase collapse under high aqueous conditions, no bonded phase bleed at high temperature, and
no phase hydrolysis.
Chromatographic Comparison** of Polymer Columns
PolymerX 5 m RP-1

Mobile Phase: 50 g tertiary butyl alcohol in
200 mL water
60 mL pH 7.5 phosphate buffer
50 mL tetrabutyl ammonium
hydrogen sulfate
10 mL EDTA disodium solution
diluted with water to 1000 mL
Temperature: 60 C
Injection Volume: 2 L
Sample: 1. 4-Epioxytetracycline
2. Oxytetracycline
3. Tetracycline
1
1
App ID 11124
**Comparative separations may not be representative of all applications.
Sample: 1. Unknown
PolymerX 7 m RP-1
2. Erythromycin related compound N
250 x 4.6 mm
3. Erythromycin C
00G-4327-EO
4. Erythromycin A
A: 1.75 g dibasic potassium
5. Erythromycin B
phosphate in 50 mL water,
adjust to pH 9.0. Add 165 mL
4
5
of tertiary butyl alcohol and
2 3
30 mL acetonitrile. Add water
to a final volume of 1 L
1
B: Acetonitrile
A/B (50:50)
0.8 mL/min
UV @ 215 nm
75 C
20 L
App ID 10406
Flow Rate:
Detection:
Temperature:
Injection Volume:
Stainless Steel Hardware Columns (mm)
Phases
50 x 4.0
150 x 2.0
150 x 4.1
3 m
00B-4338-D0
00F-4338-Z0
5 m
7 m
10 m
3
3
Erythromycins
Column:
Dimensions:
Part No.:
Mobile Phase:
Polymer Labs 5 m PLRP-S
App ID 10409
Polymerx | HPLC
Tetracyclines
150 x 4.6
250 x 4.1
250 x 4.6
250 x 10.0
250 x 21.2
00B-4326-D0 00F-4326-B0 00F-4326-Z0 00F-4326-E0 00G-4326-Z0 00G-4326-E0
00G-4327-E0
00G-4328-Z0 00G-4328-E0 00G-4328-N0 00G-4328-P0
RP-1 SecurityGuardCartridges (mm)

4 x 2.0*
4 x 3.0*
/10pk
/10pk
AJ0-5808
AJ0-5809
for ID: 2.0-3.0 mm
3.2-8.0 mm
10 x 10
/3pk
AJ0-7368
9-16 mm
For PolymerX Column Performance

Check Standards, see p. 365
Bulk media available upon request.
PLRP-S is a trademark of Polymer Laboratories, Ltd.
224
Phenomenex
PolySep -GFC-P
Aqueous GFC Columns for the

Separation of Polymers, Proteins
and Peptides
Dextran
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Sample:
Highly hydrophilic synthetic polymer phase

Suitable for water-soluble polymers
Very low nonspecific interaction with the
separation matrix
Extremely cost-effective
High efficiencies
PolySep-GFC-P4000
300 x 7.8 mm
00H-3144-K0
Water
0.8 mL/min
RI
20 L injected
1. Dextran
2. Dextran
3. Dextran
4. Dextran
5. Dextran
6. Dextran
7. Ethylene Glycol
500
110
70
40
15
6
62.07
Good mechanical strength
23 45
App ID 5452
The PolySep material undergoes rigorous quality control tests, from

the initial stages of testing of the starting monomers to the final
product. There are at least 25 steps of quality assurance during
the entire procedure. The packed column then undergoes at least
five additional tests, including a batch test for the manufactured
materials. Each column is then tested for column efficiency and
peak symmetry and shipped with the QC chromatogram for batch
test as well as column test. This ensures long-lasting columns with
very high efficiencies.
KD
KD
KD
KD
KD
KD
D
10
14
18 min
PolySep-GFC-P Technical Data and Specifications

Phase:
1000
2000
2 x 10
3.5 x 103
20 - 3 K
22,000
3
3000
9 x 10
1 x 104
100 - 10 K
50,000
3
4000
5 x 10
1 x 105
250 - 75 K
32,000
4
5000
2 x 10
3.5 x 105
3K - 400 K
32,000
5
6000
2 x 10
4 x 106
50K - 2 M
32,000
6
1 x 10
2 x 107
100K - 15 M
32,000
20 %
95 %
70 %
70 %
70 %
70 %
20 %
70 %
70 %
70 %
70 %
70 %
3.0 to 12.0
Depends on backpressure, do not exceed 650 psi
Stainless steel or PEEK (Biocompatible hardware available upon request)
4 to 60 C
Maximum allowed 0.5 M with a flow rate not to exceed 0.5 mL/min
For overnight, pump water at 0.2 mL/min, for longer storage use 0.05 % NaN3 in water
A guard column is recommended to improve column life
Linear
7
1 x 107
2 x 107
1 K - 10 M
32,000
70 %
70 %
HPLC | polysep-gfc-p / PRIMESPHERE
Exclusion Limits in Daltons:

PEG
Pullulans
Separation Range (Da)
Typical Efficiency Plates/meter
Maximum Organic Modifier:
Methanol
Acetonitrile
pH Range
Maximum Flow Rate
Column Hardware
Temperature
Maximum Salt
Storage
General
For full line of Polymer Calibration

Stainless Steel Columns (mm)

Phases
Analytical
300 x 7.8
Guards
35 x 7.8
1000
2000
3000
4000
5000
6000
Linear
00H-3141-K0
00H-3142-K0
00H-3143-K0
00H-3144-K0
00H-3145-K0
00H-3146-K0
00H-3147-K0
03Q-3248-K0
03Q-3248-K0
03Q-3248-K0
03Q-3248-K0
03Q-3248-K0
03Q-3248-K0
03Q-3248-K0
For additional GFC Columns, see p. 292
For HPLC Column Chiller/Heater System (8-70 C), see p. 357
PrimeSphere
Phenomenex
225
Prodigy
If Prodigy analytical columns do not

FULL REFUND.
Guaranteed Replacement to Inertsil

Highly reproducible
Long column life
Mimics performance of GL Sciences, Inc. Inertsil
Phenyl PH-3
ODS and C8
A Polar Phenyl-Ethyl Column
Excellent Peak Asymmetry
Polymerically bonded phenyl-ethyl phase
Prodigy Phenyl-3 (PH-3) - a polymerically bonded ethyl-linked

phenyl phase with the lowest hydrophobicity of the phenyl phases.
Low hydrophobicity and no additional polar functionality result in
aromatic interactions being most pronounced with this phase.
Luna Phenyl-Hexyl - a phenyl column with a hexyl linker and

non-polar endcapping results in a phenyl phase with significant
hydrophobicity. Such high hydrophobicity results in a phenyl phase
with strong aromatic and hydrophobic selectivity. (see p. 193)
Aq
Aq
Gemini C6-Phenyl
Prodigy 5 m C8
Peak Asymmetry =1.05
O Si O
O
Luna Phenyl-Hexyl
App ID 5455
Prodigy | HPLC
Gemini C6-Phenyl - a phenyl phase with a C6 linker and the TWIN

particle technology of Gemini results in a wide pH stability from
1-12, extremely low UV and MS bleed phenyl, and high aromatic
selectivity with exceptional peak shape. (see p. 168)
Synergi Polar-RP
Increasing Hydrophobicity
Synergi Polar-RP - an ether-linked phenyl phase with polar

endcapping that has moderate hydrophobicity. By having polar
endcapping and a polar embedded group Synergi Polar-RP has
the most polar selectivity of the phenyl phases. (see p. 249)
Prodigy Phenyl-3
App ID 5456
Additional Phenomenex Solutions for Phenyl Phase Selectivity

The ability to participate in - interactions gives Phenomenex phenyl phases selectivities not available from conventional C8 or C18
columns. Four different phenyl phases are available, each offering
unique phase characteristics:
Polar Stationary Phase Selectivity

Columns: Prodigy PH-3
Conventional C18 column
Mobile Phase: Acetonitrile/Water 45:55 for Prodigy PH-3,
65:35 for conventional C18
Sample: 1. Ethylbenzene
2. Naphthalene
Prodigy 5 m ODS (2)

Peak Asymmetry =1.01

Dimensions:
Mobile Phase:
Flow Rate:
Detection:
Sample:
App ID 10247
Reversal of elution order
Prodigy PH-3
226
vs.
150 x 4.6 mm
1. 0 mL/min
UV @ 254 nm
1. Acetophenone
2. Benzene
3. Toluene
4. Naphthalene
C18
Phenomenex
Prodigy
If Prodigy analytical columns do not

and dimensions, return the column
with comparative data within 45 days
for a FULL REFUND.
3 m ODS-3 Columns (mm)
Phases
100 x 2.0
ODS-3 100
00D-4222-B0
150 x 2.0
100 x 4.0
30 x 4.6
75 x 4.6
100 x 4.6
150 x 4.6
00F-4222-B0
00D-4222-D0
00A-4222-E0
00C-4222-E0
00D-4222-E0
00F-4222-E0
3 m and 5 m ODS-3V Columns (mm)

Phases
Part No.
3 m ODS-3V
00D-4243-E0
3 m ODS-3V
00F-4243-E0
5 m ODS-3V
00F-4241-E0
5 m ODS-3V
00G-4241-E0
Size (mm)
100 x 4.6
150 x 4.6
150 x 4.6
250 x 4.6
Price
100 x 2.0
150 x 2.0
00D-3300-B0
00D-4097-B0
00D-4298-B0
00F-3301-B0
00F-3300-B0
00F-4097-B0
00F-4298-B0
5 m Minibore Columns (mm)

Phases
50 x 2.0
C8 150
ODS-2 150
ODS-3 100
Phenyl-3 (PH-3) 100
00B-3301-B0
00B-4097-B0
00B-4298-B0

4 x 2.0*
4 x 3.0*
/10pk
/10pk
AJ0-4286
AJ0-4287
for ID: 2.0-3.0 mm
3.2-8.0 mm

250 x 2.0
4 x 2.0*
/10pk
AJ0-4289
AJ0-4286
00G-4097-B0
AJ0-4286
AJ0-4350
for ID: 2.0-3.0 mm
5 m MidBore Columns (mm)

Phases
250 x 3.0
100 x 3.2
150 x 3.2
250 x 3.2
00F-3301-Y0
00F-4097-Y0
00F-4298-Y0
00G-3301-Y0
00G-3300-Y0
00G-4097-Y0
00D-4097-R0
00F-3300-R0
00F-4097-R0
00G-3301-R0
00G-3300-R0
00G-4097-R0
5 m and 10 m Analytical Columns (mm)

Phases
30 x 4.6
5 m Silica 150
5 m C8 150
5 m ODS-2 150
5 m Silica 100
5 m ODS-3 100
5 m Phenyl-3 (PH-3) 100
10 m Silica-3 100
10 m ODS-3 100
00A-3301-E0
00A-3300-E0
00A-4097-E0
50 x 4.6
100 x 4.6
150 x 4.6
00B-3301-E0
00B-3300-E0
00B-4097-E0
00D-3301-E0
00D-3300-E0
00D-4098-E0
00D-4097-E0
00F-3301-E0
00F-3300-E0
00F-4097-E0
00F-4298-E0
5 m and 10 m SemiPreparative and Preparative Columns (mm)

Phases
250 x 10
250 x 21.2
5 m ODS-3 100
10 m ODS-3 100
10 m-PREP Columns (mm)

Phases
250 x 4.6
ODS-PREP
00G-4088-E0
00G-4097-N0
00G-4244-N0
250 x 10
00G-4088-N0
00G-4097-P0

250 x 4.6
4 x 3.0*
/10pk
00G-3302-E0
AJ0-4348
00G-3301-E0
AJ0-4290
00G-3300-E0
AJ0-4287
00G-4098-E0
AJ0-4348
00G-4097-E0
AJ0-4287
00G-4298-E0
AJ0-4351
00G-4245-E0
AJ0-4348
00G-4244-E0
AJ0-4287
for ID: 3.2-8.0 mm

10 x 10
15 x 21.2**
/3pk
/ea
AJ0-7221
AJ0-7839
AJ0-7221
AJ0-7839
for ID: 9-16 mm
18-29 mm

4 x 3.0*
10 x 10
/10pk
/3pk
AJ0-4287
AJ0-7221
for ID: 3.2-8.0 mm
9-16 mm

Phenomenex

4 x 2.0*
4 x 3.0*
/10pk
/10pk
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
AJ0-4286
AJ0-4287
AJ0-4350
AJ0-4351
for ID: 2.0-3.0 mm
3.2-8.0 mm
HPLC | Prodigy
C8 150
ODS-2 150
ODS-3 100
Phenyl-3 (PH-3) 100
150 x 3.0

227
Rezex
If Rezex columns do not provide at

least an equivalent separation as
compared to a competing column
of the same particle size, phase and
dimensions, return the column with the
FULL REFUND.
Carbohydrate and Organic Acid Analysis

Excellent resolution and column-to-column reproducibility
Easy, accurate quantitation from sharper peak shapes
Longer column lifetimes and faster run time capability from lower backpressures
Baseline separation of critical sample components due to higher efficiencies
Rezex ion-exclusion HPLC columns achieve reproducible, accurate separations based on
multiple modes of interaction. Available in 4 % and 8 % cross-linked sulfonated styrenedivinylbenzene (SDVB) and multiple ionic forms (calcium, sodium, hydrogen, potassium,
lead, and silver) for a wide range of selectivities. USP L17, L19, L22, L34, and L58 packings available.
Use Rezex for carbohydrate, oligosaccharide, and organic acid separations:
Drug formulation and excipient analysis
Food and beverage quality control testing
Recommended alternative to Bio-Rad Aminex, Supelco SUPELCOGEL, and Waters Sugar-Pak (see p.234)
Rezex
| HPLC
Find the Column For Your Application

Phases Available
Description
Applications
Additional Notes
RCM-Monosaccharide
(L19 packing)*
8 % cross-linked resin
CALCIUM ionic form
Our most commonly used column type

Easy regeneration with calcium nitrate solutions
RHM-Monosaccharide
(L17 packing)*
RAM-Carbohydrate
HYDROGEN ionic form
SILVER ionic form
SILVER ionic form
SODIUM ionic form
LEAD ionic form
SODIUM ionic form
HYDROGEN ionic form
HYDROGEN ionic form
POTASSIUM ionic form
CALCIUM ionic form
Monosaccharides and sugar alcohols from

sweeteners and corn and cane sugars
Di, tri, and tetra saccharides
Monosaccharides in combination with organic acids, fatty acids,
alcohols, ketones, neutral compounds, or inorganic salts
Selectivity complementary to other Rezex column types
High resolution of oligosaccharides up to 18 degrees of polymerization (Dp)
Guard column is recommended to

protect the ionic integrity of the matrix
RSO-Oligosaccharide
RNO-Oligosaccharide
RPM-Monosaccharide
(L34 packing)*
RNM-Carbohydrate
(L58 packing)*
ROA-Organic Acid
(L22 packing)*
RFQ-Fast Acid
RKP-Potassium
RCU-USP Sugar Alcohols
(L19 packing)*
Versatile column, generally run with a mobile phase of

deionized water
High resolution of oligosaccharides

Monosaccharides and sugar alcohol analysis. Cellobiose, glucose, xylose,
arabinose, mannose and other cellulose products
For matrices which contain high concentration of inorganic sodium,
i.e. molasses
Organic acids alone or in combination with carbohydrates, alcohols,
fatty acids, or neutral compounds; Amino sugars
Rapid screening of fruit quality; ethanol, acetic acid, glycerol, and
standard alcohol mixtures
Analysis of glyphosate
For sugar analysis according to the USP procedures
Easily regenerated to the original ionic strength

Selectivity can be altered by changing the pH as well as the
type of dilute mineral acid used as the mobile phase
Analytes are routinely chromatographed under 5 minutes
Sorbitol and mannitol can be resolved using simple isocratic

conditions
* United States Pharmacopeia (USP)
228
Phenomenex
Rezex
Bioethanol Fermentation Monitoring

Easy quantitation of ethanol fermentation broth components
Monitor starches, sugars, organic acids, and ethanol in one run
Reliable lactic acid and acetic acid monitoring
Increase throughput by reducing run times 50 % with 150 mm column length
Monitoring the key reaction components throughout the fermentation process is crucial for maximizing ethanol recovery. Rezex ROA
is uniquely suited for the separation and analysis of simple and
complex sugars, organic acids, and ethanol within a fermentation
broth sample. With results easily obtained through an isocratic run,
Rezex ROA is instrumental in helping you to accurately determine
what critical steps need to be taken to ensure the maximum yield is
achieved during your fermentation run.
Rezex ROA has the ability to achieve excellent baseline separation between Dp3 and Dp4+, which have proven to be a challenge
within the bioethanol industry. It is this great baseline separation
that affords scientists the opportunity to utilize a shorter column
dimension. By using the 150 x 7.8 mm Rezex ROA column, you
are able to decrease the run time by 50 % when compared to the
average run time on a 300 x 7.8 mm column.
Great Resolution of
Dp4+ and Dp3!
Reduce run times by 50% with

150 mm column length
8
3
10
20
10
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
System:
Sample:
Column: 1Rezex ROA-Organic Acid

Part No.: 00H-0138-K0
Mobile Phase: 0.005 N Sulfuric Acid
Detection: RI @ 40 C
3 4
Temperature: 60 C
System: Shimadzu Prominence LC-20A System
Sample: 1. Dp4+
5. Lactic Acid
2. Dp3
6. Glycerol
3. Maltose
7. Acetic Acid
4. Glucose
8. Ethanol
6
2
5
min
20
min
Shorten GC Fuel Quality Testing

Zebron ZB-Bioethanol GC column can shorten your
quality test down to 5 minutes! (See p. 91).
8
App ID 16648
App ID 16650
6
7
10
12
HPLC | Rezex
14 min

150 x 7.8 mm
00F-0138-K0
0.005 N Sulfuric Acid
0.6 mL/min
RI @ 40 C
60 C
Shimadzu Prominence LC-20A System
1. Dp4+
5. Lactic Acid
2. Dp3
6. Glycerol
3. Maltose 7. Acetic Acid
4. Glucose 8. Ethanol
Extend Column Lifetime

Protect the Rezex column from the intrusion of the metal
ions by using Phenex Syringe Filters and SecurityGuard.
The filters and SecurityGuard guard cartridge system work
by trapping metal ions, such as calcium, magnesium, and
iron, which can damage the column and cause it to lose or
change separation efficiency. (See pp. 10 and 235).
6
1
Phenomenex
3
2
229
Rezex
Rezex vs. Bio-Rad Aminex

Phenomenex guarantees satisfaction when using Rezex HPLC columns. As
illustrated below, Rezex offers advantages that enhance chromatographic
results, increase throughput, and simplify quantitation.
Easier, Accurate Quantitation

Due to improved peak shape
Saccharides
Orange Juice

Column: Rezex RCM-Monosaccharide
Aminex HPX-87C
Mobile Phase: Water
Detection: ELSD
Temperature: 80 C
Sample: 1. Melezitose
4. Mannose
2. Maltose
5. Fructose
3. Glucose
6. Ribitol

Aminex HPX-87C
Mobile Phase: Water
Detection: ELSD
Temperature: 80 C
Sample: 1. Sucrose
2. Glucose
3. Fructose
Superior
Peak Shape
Rezex RCM
10
100
4
3
40
0
8
1
Aminex HPX-87C
400
App ID 14976
mAU
| HPLC
60
10
100
80
10
12
14
2
0
20 min
10
12
14
Due to lower backpressures
Baseline Separation of Critical

Sample Components
Aliphatic Acids
Due to improved resolution

Sugars

Column: Rezex ROA-Organic Acid
Aminex HPX-87H
Mobile Phase: 0.005 N H2SO4
Temperature: 40 C
Sample: 1. Oxalic Acid
4. Succinic Acid
2. Citric Acid
5. Formic Acid
3. Tartaric Acid
6. Acetic Acid

Aminex HPX-87C
Mobile Phase: Water
Detection: ELSD
Temperature: 80 C
Sample: 1. Sucrose
4. Fructose
2. Glucose
5. Sorbitol
3. Galactose
Rezex RCM
60
40
20
80
mAU
32 Bar
80
Baseline
Resolution
100
mAU
Rezex ROA
60
40
0
0
10
20 min
Aminex HPX-87H
mAU
80
60
40
20
10
30 min
Aminex HPX-87C
60
2
40
0
20
80
mAU
55 Bar
100
10
100
App ID 14963
120
16 min
App ID 14977
Longer Column Lifetime
120
16 min
Aminex HPX-87C
Difficult
Quantitation
200
App ID 14962
Rezex
200
20 min
6
App ID 14981
40
300
App ID 14982
60
mAU
mAU
mAU
400
80
App ID 14975
100
Rezex RCM
1
500
10
20
App ID 14978
120
30 min
20 min
230
Phenomenex
Rezex

300 x 7.8 mm
00H-0138-K0
0.005 N Sulfuric Acid
0.5 mL/min
UV @ 210 nm
55 C
1. Oxalic
2. Citric
3. Tartaric
4. Succinic
5. Formic
6. Acetic
45
1 3
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
300 x 7.8 mm
00H-0130-K0
Water
0.6 mL/min
Rl
85 C
1. Melezitose
2. Maltose
3. Glucose
4. Mannose
5. Fructose
6. Ribitol
6
4 5
2 3
App ID 5515
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
Saccharides
App ID 5504
Organic Acids
24 min
12
Apple Juice
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
3
1
Rezex RKP-Potassium
300 x 7.8 mm
00H-3252-K0
Water
0.4 mL/min
RI
85 C
1. Maltotriose
2. Maltose
3. Glucose
12
24
36 min
Oligosaccharides
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
HPLC | Rezex
App ID 5505
App ID 5506
300 x 7.8 mm
00H-0130-K0
Water
0.6 mL/min
RI
75 C
1. Sucrose
2. Glucose
3. Fructose
Carbohydrates
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
16 min
20 min
10
Saccharides
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
Rezex RSO-Oligosaccharide
200 x 10 mm
00P-0133-N0
Water
0.3 mL/min
Rl
75 C
Malto-Oligosaccharides as shown
Dp2
Rezex RPM-Monosaccharide
300 x 7.8 mm
00H-0135-K0
Water
0.6 mL/min
RI
75 C
1. Stachyose
2. Maltose
3. Glucose
3 5
4. Xylose
4
5. Galactose 2
6
6. Fructose
Dp3
7.
8.
9.
10.
11.
Meso-Erythritol
Mannitol
Salicin
Xylitol
Sorbitol
8
10
Dp4
11
9
Dp5
Dp9
Dp8 Dp6
Dp7
Dp refers to Degree of polymerization
Phenomenex
Dp1
35
App ID 5508
App ID 5507
Dp10
Dp11
Dp12
Dp13
Dp14
min
10
20
30
40 min
231
Rezex
14987, 14988, 14989
300 x 7.8 mm
00H-0130-K0
Water
0.5 mL/min
ELSD
1
80 C
1. Sucrose
2. Glucose
3. Galactose
4. Fructose
3
15501
1
3
23
2
4
10 min
10 min
10 min
10
12
14
16
min
Carboxylic Acids
300 x 7.8 mm
00H-0136-K0
Water
0.6 mL/min
RI
75 C
1. Stachyose
2. Cellobiose
3. Glucose
4. Fructose
5. Arabinose
6. Ribose
7. Salicin
(1 % solution of each)
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
2
3
4
5

300 x 7.8 mm
00H-0138-K0
Water + 0.5 % Trifluoroacetic Acid
1.0 mL/min
RI
2
40 C
1. Acetylene Carboxylic Acid
2. Maleic Acid
3. Succinic Acid
4. Fumaric Acid
1
7
6
App ID 5510
| HPLC
Rezex
Rezex RPM-Monosaccharide
100 x 7.8 mm
00D-0135-K0
Water
0.6 mL/min
ELSD
1
80 C
1. Mannitol
2. Sorbitol
2
Carbohydrates
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
App ID 15501
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
Mannitol and Sorbitol
App ID 5517
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
App ID 14987, 14988, 14989
Fermentation Broth
Food Softeners
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
25 min
16
24 min
Amino Sugars
300 x 7.8 mm
00H-0130-K0
Water
0.5 mL/min
RI
60 C
1. Glycerol
2. Methoxypolyethylene Glycol
3. Triethylene Glycol
4. Sorbitol
5. Urea
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
1

300 x 7.8 mm
00H-0138-K0
1 % Phosphoric Acid
0.6 mL/min
RI
Ambient
1. Glucose
2. N-Acetylglucosamine
3. N-Acetylgalactosamine
2
1
0.00
232
10.00
20.00
30.00 min
App ID 5514
App ID 5518
10
20
min
Phenomenex
Rezex
Specifications and Operating Recommendations

Part Number
Ionic Form
Standard Dimensions
Matrix
Cross Linking
Particle Size
Min. Efficiency (p/m) based
on last peak
Typical Pressure
(psi @ Max Flow Rate)
Max. Pressure
Max. Flow Rate (mL/min)
Max. Temperature (C)
Typical Mobile Phase
pH Range
Guard Column Part No.
RCM-Monosaccharide
RSO-Oligosaccharide
RNO-Oligosaccharide
RNM-Carbohydrate
RAM-Carbohydrate
00H-0130-K0
Calcium
300 x 7.8 mm
00P-0133-N0
Silver
200 x 10 mm
00H-0136-K0
Sodium
300 x 7.8 mm
00H-0131-K0
Silver
300 x 7.8 mm
8%
8 m
35,000
4%
12 m
N/A
00P-0137-N0
Sodium
200 x 10 mm
Sulfonated Styrene-divinylbenzene
4%
12 m
N/A
8%
8 m
30,000
8%
8 m
35,000
400
200
200
400
400
1,000
300
300
1,000
1,000
1.0 (see pressure)

85
Water
Neutral
03B-0130-K0
0.3
85
Water
Neutral
03R-0133-N0
0.3
85
Water
Neutral
03R-0137-N0
1.0
85
Water
Neutral
03B-0136-K0
1.0
85
Water
Neutral
03B-0131-K0
5 % Silver Nitrate
10 % Methanol, IPA, EtOH, Acetonitrile

5 % Sodium Salts
5 % Sodium Salts
2 % Silver Nitrate
Cleaning, Regeneration and Storage

Organic Modifiers (Max)
Inorganic Modifiers
Avoid
5 % CaSO4, Ca(NO3)2, CaCl2

Acids, Bases, Non-Calcium
Salts/ Metal Ions,
>10 % Organic
Acids, Bases, Non-Silver Salts/

Metal Ions, >5 % Organic
100 % Water
0.6
85
12
0.1 M Ca(NO3)2
0.2
85
4-16
Water
100 % Water
0.2
75
12
0.1 M AgNO3
0.1
85
4-16
Water
100 % Water
0.2
75
12
0.1 M NaNO3
0.2
85
4-16
Water
100 % Water
0.4
75
12
0.1 M NaNO3
0.2
85
4.-16
Water
100 % Water
0.4
75
12
0.1 M AgNO3
0.2
85
4-16
Water
RPM-Monosaccharide
RHM-Monosaccharide
ROA-Organic Acid
RFQ-Fast Acid
RCU-Sugar Alcohols
00H-0135-K0
Lead
300 x 7.8 mm
00H-0132-K0
Hydrogen
300 x 7.8 mm
00D-0223-K0
Hydrogen
100 x 7.8 mm
00G-0130-D0
Calcium
250 x 4.0 mm
8%
8 m
35,000
8%
8 m
35,000
00H-0138-K0
Hydrogen
300 x 7.8 mm
Sulfonated Styrene-divinylbenzene
8%
8 m
50,000 (Acetic Acid)
8%
8 m
30,000
8%
8 m
12,000
400
400
400
400
400
1,000
1,000
1,000
1,000
1,000
1.0
85
Water
Neutral
03B-0135-K0
1.0
85
Water
1-8
03B-0132-K0
1.0
85
0.005 N H2SO4
1-8
03B-0138-K0
1.0
85
0.005 N H2SO4
1-8
03B-0223-K0
0.5
85
Water
Neutral
03A-0130-D0
5 % Lead Nitrate
5 % HNO3, H3PO4
10 % Methanol, IPA, EtOH, Acetonitrite

5 % HNO3, H3PO4
5 % HNO3, H3PO4
Acids, Bases, Non-Lead Salts/

Acids, Bases, Salts/

Acids, Bases, Salts, Metal Ions,

pH > 3, >10 % Organic
Acids, Bases, Salts, Metal Ions,

pH > 3, >10 % Organic
100 % Water
0.6
85
12
0.1 M Pb(NO3)2
0.2
85
4-16
Water
100 % Water
0.6
85
12
0.025 M H2SO4
0.2
85
4-16
Water
100 % Water
0.6
85
12
0.025 M H2SO4
0.2
85
4-16
0.005 N H2SO4
100 % Water
0.6
85
12
0.025 M H2SO4
0.2
85
4-16
0.005 N H2SO4
5 % CaSO4, Ca(NO3)2, CaCl2

Acids, Bases, Non-Calcium
Salts, or Metal Ions,
>10 % Organic
100 % Water
0.2
85
12
0.1 M Ca(NO3)2
0.2
85
4-16
Water
Part Number
Ionic Form
Standard Dimensions
Matrix
Cross Linking
Particle Size
Min. Efficiency (p/m) based
on last peak
Typical Pressure
Max. Pressure
Max. Flow Rate (mL/min)
Max. Temperature (C)
Typical Mobile Phase
pH Range
Guard Column Part No.
Acids, Bases, Non-Silver Salts/

HPLC | Rezex
Cleaning Solvent
Flow Rate(mL/min)
Temperature (C)
Duration (hrs)
Regeneration Solvent
Flow Rate (mL/min)
Temperature (C)
Duration (hrs)
Ship/Storage Solvent
Acids, Bases, Non-Sodium Salts/ Acids, Bases, Non-Sodium Salts/

Metal Ions,
Metal Ions,
>5 % Organic
>10 % Organic
Cleaning, Regeneration and Storage

Organic Modifiers (Max)
Inorganic Modifiers
Avoid
Cleaning Solvent
Flow Rate(mL/min)
Temperature (C)
Duration (hrs)
Regeneration Solvent
Flow Rate (mL/min)
Temperature (C)
Duration (hrs)
Ship/Storage Solvent
Phenomenex
233
Rezex
If Rezex columns do not provide at

least an equivalent separation as
compared to a competing column
of the same particle size, phase and
dimensions, return the column with the
FULL REFUND.
Retention Times for Some Carbohydrates and Sugar Alcohols
Rezex
| HPLC
Counter Ion Analyte
RAM Ag+
Adonitol (Ribitol)
D-Altrose
D-(-)-Arabinose
D-(+)-Cellobiose
D-(+)-Digitoxose
Dulcitol
Meso-Erythritol
D-(-)- Fructose
L-(-)-Fucose
D-(+)-Galactose
Gentiobiose
D-(+)-Glucose
Inositol
Isomaltose
Lactose
Lactulose
D- Lyxose
D- Maltose
Maltotriose
Maltulose
D- Mannitol
D-(+)-Mannose
Melibiose
D-(+)-Melezitose
D-(+)-Raffinose
L-(+)-Rhamnose
D-(-)-Ribose
Salicin
D-Sorbitol
Stachyose
Sucrose
Trehalose
Xylitol
D-(+)-Xylose
* Partial hydrolysis results.
11.54
11.95
13.01
8.86
11.90
11.64
12.31
12.05
12.75
11.87
8.70
11.04
12.59
9.11
9.27
9.75
12.41
9.16
8.27
9.25
11.36
12.04
9.26
8.00
8.10
11.50
14.59
18.51
11.91
7.60
9.03
8.91
12.69
12.06
RCM Ca+2
RNM Na+
RHM H+
RPM Pb+2
14.93
12.71
13.56
8.60
13.82
21.61
15.49
13.65
13.19
11.73
8.40
10.37
13.35
8.74
9.03
10.32
14.06
8.81
8.10
9.47
17.82
12.04
9.04
7.93
8.16
12.18
23.38
18.58
22.45
7.59
8.71
8.72
22.01
11.62
11.10
11.45
12.65
8.49
11.39
11.10
11.78
11.76
12.30
11.47
8.40
10.71
12.14
8.76
8.78
9.23
11.98
8.75
7.94
8.82
10.80
11.54
8.82
7.66
7.76
11.00
14.34
17.36
11.39
7.30
8.65
8.49
12.16
11.68
11.11
10.21
11.24
8.02
12.59
10.71
12.14
10.31
11.65
10.19
7.87
9.62
9.98
8.02
8.32
8.57
10.68
8.18
7.51
8.27
10.59
10.16
8.14
7.54*
7.88*
10.90
11.42
14.98
10.83
7.27
9.24*
8.32
11.78
10.24
20.15
15.82
16.47
11.00
15.32
33.25
19.82
17.71
16.19
14.94
10.53
12.92
18.87
11.28
11.89
13.95
16.66
11.59
11.02
12.40
24.90
16.39
11.97
9.94
10.28
14.47
33.48
26.81
35.97
9.72
11.00
11.01
32.38
13.84
Conditions:
Dimensions:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
300 x 7.8 mm
Water (degassed)
0.6 mL/min
80 C
RI @ 40 C
Column Cross Reference Chart

Phenomenex
Rezex
Bio-Rad
Aminex
Supelco
Supelcogel
Waters
Sugar-Pak
RCM-Monosaccharide
HPX-87C
125-0095
HPX-87H
125-0140
HPX-87P
125-0098
HPX-87N
125-0143
HPX-42A
125-0097
HPX-87H
125-0140
Fast Acid
125-0100
HPX-87K
125-0142
Sugar Alcohols
125-0094
Supelcogel Ca
Sugar-Pak 1
Supelcogel C-610H & H
N/A
Supelcogel Pb
N/A
N/A
N/A
Supelcogel Ag1 & Ag2
N/A
Supelcogel C-610H & H
N/A
N/A
N/A
Supelcogel K
N/A
N/A
N/A
RHM-Monosaccharide
RPM-Monosaccaride
RNM-Carbohydrate
RSO-Oligosaccharide
ROA-Organic Acid
RFQ-Fast Acid
RKP-Potassium
Columns
Guards
Description
RCM-Monosaccharide
RCM-Monosaccharide
RHM-Monosaccharide
RHM-Monosaccharide
RAM-Carbohydrate
RSO-Oligosaccharide
RSO-Oligosaccharide
RNO-Oligosaccharide
RPM-Monosaccharide
(for USP procedure)
RPM-Monosaccharide
RNM-Carbohydrate
ROA-Organic Acid
ROA-Organic Acid
ROA-Organic Acid
ROA-Organic Acid
RKP-Potassium
RFQ-Fast Acid
Part No.
00H-0130-K0
00G-0130-P0
00H-0132-K0
00G-0132-P0
00H-0131-K0
00P-0133-N0
00G-0133-P0
00P-0137-N0
00H-0135-K0
00D-0135-K0
00G-0135-P0
00H-0136-K0
00H-0138-K0
00F-0138-K0
00G-0138-E0
00G-0138-P0
00H-3252-K0
00D-0223-K0
00G-0130-D0
Cross
Linkage
8%
8%
8%
8%
8%
4%
4%
4%
8%
8%
8%
8%
8%
8%
8%
8%
8%
8%
8%
Ionic Form
Calcium
Calcium
Hydrogen
Hydrogen
Silver
Silver
Silver
Sodium
Lead
Lead
Lead
Sodium
Hydrogen
Hydrogen
Hydrogen
Hydrogen
Potassium
Hydrogen
Calcium
Size (mm)
300 x 7.8
250 x 21.2
300 x 7.8
250 x 21.2
300 x 7.8
200 x 10.0
250 x 21.2
200 x 10.0
300 x 7.8
100 x 7.8
250 x 21.2
300 x 7.8
300 x 7.8
150 x 7.8
250 x 4.6
250 x 21.2
300 x 7.8
100 x 7.8
250 x 4.0
Price
Part No.
03B-0130-K0
03B-0132-K0
03R-0133-N0
03R-0137-N0
03B-0135-K0
03B-0135-K0
03B-0136-K0
03B-0138-K0
03B-0138-K0
03B-0223-K0
03A-0130-D0
Size (mm)
50 x 7.8
50 x 7.8
60 x 10.0
60 x 10.0
50 x 7.8
50 x 7.8
50 x 7.8
50 x 7.8
50 x 7.8
50 x 7.8
30 x 4.0
Price

4 x 3.0*
15 x 21.2**
/10pk
ea
AJ0-4493
AJ0-8889
AJ0-4490
AJ0-8888
AJ0-4491
AJ0-8891
AJ0-4492
AJ0-4492
AJ0-8890
AJ0-4490
AJ0-4490
AJ0-4490
AJ0-8888
AJ0-4490
AJ0-4493
for ID: 3.2-8.0 mm

18.0-29.0 mm
*SecurityGuard Analytical Cartridges require universal holder Part No.: KJO-4282

**PREP SecurityGuard Cartridges require holder, Part No. AJ0-8223
For our full line of Column Performance Check Standards, see pp. 365-366
234
Phenomenex
SecurityGuard
U.S. Patent Nos. 5, 863, 428 and 6, 162, 362
Column Protection for Analytical to Prep

Protect HPLC columns and extend lifetime
If the SecurityGuard Cartridge System

does not provide at least an equivalent
performance as compared to a competing guard cartridge system, return
the product with the comparative data
Virtually no change in chromatography

Available in analytical, semi-prep, and preparative sizes
Simple to use
Did you know a common cause of high backpressure, split
peaks, broad peaks, baseline noise, baseline drift and loss of
resolution is contaminants? The fact is all mobile phases contain
some chemical contaminants or microparticulates, from the
sample, solvent, or wear on the polymeric seals of the pump or
injector. These contaminants can clog frits, irreversibly bind to an
HPLC column, degrade performance, and even damage the flow
cell. An easy solution, SecurityGuard is a universal HPLC guard
cartridge system designed to effectively (and inexpensively),
protect your valuable columns, from the damaging effects of
chemical contaminants, without altering your chromatographic
results.
Fingertight connection to
virtually any female inverted
endfitting worldwide
A Universal Guard Cartridge System

How It Works*
The SecurityGuard analytical cartridge holder (patented) directly finger-tightens into virtually any manufacturers column. Contaminants are
retained by an inexpensive, 4 mm, disposable cartridge instead of fouling your expensive analytical column.
Cutaway view showing cartridge
can be easily inspected for contaminants
Universal Fingertight connection to HPLC column

no wrenches required
contaminants
compounds of interest
From injector
Flow
To detector
HPLC column
Increases HPLC Column Lifetime,

Guaranteed!
Simply replace SecurityGuard cartridges instead of your expensive
HPLC columns In this graph, once the expired SecurityGuard cartridge was replaced, the pressure immediately dropped and the column performance was restored allowing for extended column use.
Cartridge Replaced
110
100
90
80
Pressure
70
60
50
40
30
For available Semi-Preparative and PREP sizes, see pp. 237-238

For UHPLC column protection, see SecurityGuard ULTRA p. 239
20
10
0
-10
* Feature applies to analytical-sized guard system only, and does not

apply to SemiPrep or PREP guard cartridges.
Phenomenex
Injections
Accelerated lifetime test using endogenous biomolecule matrix Inertsil ODS-3, 5m,
50 x 4.6mm with SecurityGuard C18 cartridges
235
SecurityGuard
U.S. Patent Nos. 5, 863, 428 and 6, 162, 362

Compare, Virtually No Change
The problem with most guards is that you dont know when to
change them. The only indication you have that its time to replace a
typical guard is noticing a loss of resolution, a decline in efficiency,
or a rise in backpressure. By that time, its usually too late. If your
guard becomes saturated with contaminants, they can break
through and foul your valuable analytical column, equipment, and
your results.
mAU
The SecurityGuard cartridges clever design eliminates this

frustrating guesswork. The direct-view feature gives you the
opportunity to inspect the surface of the cartridges packing
material any time, without disturbing the packing bed. Now you can
easily monitor visual contaminant build-up, and change your guard
before its too late!
-30
10
0
-10
Without SecurityGuard
-20
App ID 10731
See Your Dirt Feature
-40
0
10
15
vs.
mAU
10
CLEAN:
If it looks clean, the cartridge may

be reinserted for further use.
20 min
-10
With SecurityGuard
-30
DIRTY:
-40
If either discoloration or particle build-up is

observed, its time to replace the cartridge.
SecurityGuard | HPLC
App ID 10731
-20
If your contaminants are colorless, replace the cartridge as often as

needed to maintain chromatographic performance.
The visible indicator is a great tool to help gauge when to

change the guard column!
P. Alanen
The SecurityGuard is easy to use and cartridge replacement
is simple.
F. Shakir
10
Columns:
Dimensions:
Guard Cartridge:
Mobile Phase:
Flow Rate:
Detection:
Sample:
15
20 min
As specified within App ID

150 x 4.6 mm
SecurityGuard C18 (ODS) 4 x 3.0 mm ID
0.1 M Ammonium acetate/Acetonitrile/Methanol (35:25:40)
0.6 mL/min
UV @ 245 nm
1. Clonazepam
4. Bromazepam
2. Oxazepam
5. Temazepam
3. Diazepam
6. Medazepam
We didnt see any change in retention time or difference

in the peaks. The SecurityGuard has increased the life
of the column.
B. Dietz
*Kit KJ0-4282 Includes:
Analytical Holder Kit

and Accessories
PEEK Ferrules (Qty. 3)
For 2.0 and 3.0 mm ID cartridges, use with 2.0 to 8.0 mm

ID columns
Analytical Kit
Part No.
Description
KJ0-4282
SecurityGuard Kit* (includes holder)
Replacement Parts and Accessories
Part No.
Description
AJ0-4283
PEEK Ferrules
AJ0-4285
Stacking Rings
AQ0-1389
PEEK Fingertight Fittings
AJ0-4284
SecurityGuard Wrenches
236
Price
Unit
3/pk
2/pk
10/pk
2/pk
Price
Cartridge Holder
(Qty. 1)
Stacking Rings
(to stack 2 cartridges
for extra protection) (Qty. 2)
Wrenches (Qty. 2)
PEEK
Fingertight Fittings (Qty. 2)
Phenomenex
SecurityGuard
Semi-Preparative Holder
For 10.0 mm ID cartridges, use with 9 to 16 mm ID columns
SemiPrep Guard Cartridge Holder
Part No.
Description
AJ0-7220
Holder for 10.0 mm ID cartridges
Accessories
Part No.
Nut and Ferrule
AQ0-3018
Description
10-32 Threaded Male Nut and Ferrule

Set for 1/16 in. OD capillary tubing
Sure-Lok Fingertight Fittings
AQ0-1388
PEEK Sure-Lok Male Nut
AQ0-1389
PEEK Sure-Lok Male Nut
Sure-Lok Couplers
AQ0-1392
AQ0-1393
Column Sealing Plugs
AQ0-0217
Column Sealing Plug, 10-32 Thread size
Unit
ea
Unit
Price
Price
Holder
Cartridge
(10 x 10 mm ID)
ea
ea
10/pk
ea
10/pk
10/pk
For Semi-Preparative and Preparative Cartridges, see p. 238

Prep Guard Cartridge Holder
Part No.
Description
AJ0-8223
Holder Kit for 21.2 mm ID cartridges,
includes column coupler
Unit
ea
Price
Cartridge
Holder
(15 x 21.2 mm ID)
HPLC | SecurityGuard
Preparative Holder (Two Sizes)

Prep Guard Cartridge Holder
Part No.
Description
AJ0-8277
Holder Kit for 30.0 mm ID cartridges,
includes column coupler
Replacement Parts and Accessories
Part No.
Description
AQ0-8374
PREP Coupler, SS w / PEEK Ferrule Inserts,
10-32 Threads, 1/16 in. OD x 0.020 in. ID
AQ0-8375
Replacement Ferrule Inserts, for PREP
Coupler, PEEK, 0.020 in. ID
AQ0-8222
PREP Replacement O-Rings, Kalrez
For 15 x 21.2 mm SG Holder, Size 2-021
AQ0-8318
PREP Replacement O-Rings, Kalrez
For 15 x 30 mm SG Holder, Size 2-025
AT0-0465
Capillary S.S. Tubing, 0.020 in. ID x 0.062
in. (1/16 in.) OD x 10 cm length
AT0-0466
Capillary S.S. Tubing, 0.020 in. ID x 0.062
in. (1/16 in.) OD x 20 cm length
Phenomenex
Unit
ea
Price
Cartridge
Unit
ea
Price
(15 x 30.0 mm ID)
Holder
10/pk
2/pk
2/pk
5/pk
5/pk
237
If the SecurityGuard Cartridge System does not

provide at least an equivalent performance as
compared to a competing guard cartridge system, return the product with the comparative
SecurityGuard
Cartridges and Holders

Step 1: Choose column ID
Step 2: Match column phase
2.0 - 3.0
Choose your HPLC Column Internal Diameter, ID (mm):

3.2 - 8.0
9.0 - 16.0
18.0 - 29.0
Analytical
4.0 x 2.0
SemiPrep
4.0 x 3.0
Use Cartridges (mm):

10 x 10.0
30.0 - 49.0
PREP
15 x 21.2
15 x 30.0
SecurityGuard | HPLC
Material
Description
pH Stability
Cartridges for General Purpose/Pharmaceutical
/10pk
/10pk
/3pk
ea
ea
C18
(ODS, Octadecyl)
1.5 - 10
AJ0-4286
AJ0-4287
AJ0-7221
AJ0-7839
AJ0-8301
C12
(Dodecyl)
1.5 - 10
AJ0-6073
AJ0-6074
AJ0-7275
AJ0-7842
AJ0-8304
C8
(MOS, Octyl)
1.5 - 10
AJ0-4289
AJ0-4290
AJ0-7222
AJ0-7840
AJ0-8302
C5
(Pentyl)
1.5 - 10
AJ0-4292
AJ0-4293
AJ0-7372
C1
(TMS)
2-9
AJ0-4298
AJ0-4299
AJ0-7373
Silica
AJ0-4347
AJ0-4348
AJ0-7223
AJ0-7229
AJ0-8312
HILIC
HILIC
1.5 - 8
AJ0-8328
AJ0-8329
AJ0-8902
NH2
(Amino, Aminopropyl)
1.5 - 11
AJ0-4301
AJ0-4302
AJ0-7364
AJ0-8162
AJ0-8309
CN
(Cyano, Cyanopropyl)
2 - 7.5
AJ0-4304
AJ0-4305
AJ0-7313
AJ0-8220
AJ0-8311
Phenyl
(Phenylhexyl)
1.5 - 10
AJ0-4350
AJ0-4351
AJ0-7314
AJ0-7841
AJ0-8303
PFP(2)
Pentafluorophenyl
1.5 - 8
AJ0-8326
AJ0-8327
AJ0-8376
AJ0-8377
AJ0-8378
SCX
(SA, Strong Cation Exchanger)
2.5 - 7.5
AJ0-4307
AJ0-4308
AJ0-7369
AJ0-8595
AJ0-8596
SAX
(SB, Strong Anion Exchanger)
2.5 - 7.5
AJ0-4310
AJ0-4311
AJ0-7370
RP-1
(Reversed Phase - Polymer)
0 - 14
AJ0-5808
AJ0-5809
AJ0-7368
AJ0-8358
Polar-RP
(Ether-linked Phenyl)
1.5 - 7
AJ0-6075
AJ0-6076
AJ0-7276
AJ0-7845
AJ0-8307
Fusion-RP
(C18 Polar Embedded)
1.5 - 10
AJ0-7556
AJ0-7557
AJ0-7558
AJ0-7844
AJ0-8306
AQ C18
(Polar Endcapped C18)
1.5 - 7.5
AJ0-7510
AJ0-7511
AJ0-7512
AJ0-7843
AJ0-8305
Gemini-NX
(C18 Twin-NX Technology)
1 - 12
AJ0-8367
AJ0-8368
AJ0-8369
AJ0-8370
AJ0-8371
Gemini C18
(C18 Twin Technology)
1 - 12
AJ0-7596
AJ0-7597
AJ0-7598
AJ0-7846
AJ0-8308
Gemini C6-Phenyl
(C6-Phenyl Twin Technology)
1 - 12
AJ0-7914
AJ0-7915
Oligo-RP
(C18 Twin Technology)
1 - 12
AJ0-8134
AJ0-8135
AJ0-8136
AJ0-8210
AJ0-8310
Oligo-WAX
(WA, Weak Anion Exchanger)
1.5 - 11
AJ0-8324
AJ0-8325
AJ0-8639
AJ0-8420
Cartridges for Protein and Polypeptide Reversed Phase
/10pk
/10pk
/3pk
ea
ea
For use with silica columns for separation of proteins & peptides, such as Jupiter (Phenomenex); Vydac 218TP, 214TP (Alltech Associates, Inc.); Nucleosil 300 C18, C4; Hypersil 300
, and other widepore or 300 brands.
Widepore C18
(ODS, Octadecyl)
1.5 - 10
AJ0-4320
AJ0-4321
AJ0-7224
AJ0-7230
AJ0-8313
Widepore C5
(Pentyl)
1.5 - 10
AJ0-4326
AJ0-4327
AJ0-7371
Widepore C4
(Butyl)
1.5 - 10
AJ0-4329
AJ0-4330
AJ0-7225
AJ0-7231
AJ0-8314
Cartridges for Silica GFC
/10pk
/3pk
ea
(Aqueous SEC) For use with silica GFC columns, such as BioSep (Phenomenex); ZORBAX GF-Series; Bio-Sil (Bio-Rad).
GFC-2000
2 - 7.5
AJ0-4487
AJ0-7365
AJ0-8588
GFC-3000
2 - 7.5
AJ0-4488
AJ0-7366
AJ0-8589
GFC-4000
2 - 7.5
AJ0-4489
AJ0-7367
AJ0-8590
Cartridges for Chiral

/10pk
/10pk
/3pk
ea
ea
For use with chiral columns, such as Lux Cellulose-1, -2, -3, -4, & Amylose-2 (Phenomenex); CHIRALCEL OD-H, CHIRALCEL OJ-H& Chiralpak Ad-H (DAICEL Chemical Industries,
Ltd.)
Lux Cellulose-1
Cellulose tris (3, 5-dimethyl2-9
AJ0-8402
AJ0-8403
AJ0-8404
AJ0-8405
AJ0-8406
phenylcarbamate)
Lux Cellulose-2
Cellulose tris (3-chloro-42-9
AJ0-8398
AJ0-8366
AJ0-8399
AJ0-8400
AJ0-8401
methylphenylcarbamate)
Lux Cellulose-3
Cellulose tris (4-methylbenzoate) 2 - 9
AJ0-8621
AJ0-8622
AJ0-8623
AJ0-8624
AJ0-8625
Lux Cellulose-4
Cellulose tris (4-chloro-3-methyl- 2 - 9
AJ0-8626
AJ0-8627
AJ0-8628
AJ0-8629
AJ0-8630
phenylcarbamate)
Lux Amylose-2
Amylose tris (5-chloro-2-methyl- 2 - 9
AJ0-8471
AJ0-8470
AJ0-8472
AJ0-8473
AJ0-8474
phenylcarbamate)
/10pk
ea
Cartridges for Carbohydrate/Organic Acid
For organic acid and carbohydrate analysis, such as Rezex (Phenomenex); Aminex (Bio-Rad); Interaction; Sugar-Pak (Waters).
Carbo-H+
1-8
AJ0-4490
AJ0-8888
Carbo-Ag+*
Neutral
AJ0-4491
AJ0-8891
Carbo-Pb+2
Neutral
AJ0-4492
AJ0-8890
Carbo-Ca+2
Neutral
AJ0-4493
AJ0-8889
Guard Cartridge Holders (one-time purchase only)

/kit
/holder
/kit
/kit
*For use with saccharide and oligosaccharide columns in Ag+ form.
KJ0-4282
AJ0-7220
AJ0-8223
AJ0-8277
Traditional SecurityGuard cartridges are pressure rated to
3000 psi (207 bar). For UHPLC and all applications at higher
pressures, use SecurityGuard ULTRA, see p. 239.
238
Phenomenex
SecurityGuard ULTRA
UHPLC Column Protection

Extends HPLC, core-shell and sub-2m column lifetime
Cartridge
Holder
Virtually no change in chromatography

Fits virtually all manufacturers columns 2.1 to 4.6 mm ID
Simple to use
Holder with cartridge, assembled
Universal Fit
Use SecurityGuard ULTRA with virtually all UHPLC columns 2.1 to
4.6mm ID. The extremely low dead volume of this unique product
minimizes sample peak dispersion. It will efficiently remove microparticulates and chemical contaminants from the flow stream
without contributing to system backpressure or dead volume
(<0.3L).
SecurityGuard ULTRA Increases Column Lifetime,

Guaranteed!
When contaminates and particulates build up at the head of your
column, system backpressures can increase dramatically. By simply replacing the SecurityGuard ULTRA cartridge, instead of your
column, you are able to regain normal operating conditions and
reclaim original column performance.
Accelerated lifetime test using endogenous biological matrix on
Kinetex 2.6 m C18 50 x 4.6 mm ID column
SecurityGuard ULTRA Performance
SecurityGuard ULTRA Cartridge Holder

Part No.
Description
AJ0-9000
SecurityGuard ULTRA Cartridge Holder
Unit
ea
Price
SecurityGuard ULTRA Cartridges

Material
C18
C8
PFP
HILIC
Phenyl
Description
pH Stability
(ODS, Octadecyl)
(MOS, Octyl)
(Pentafluorophenyl)
(HILIC)
(Phenyl-Hexyl)
1.5 8.5*
1.5 8.5*
1.5 8.5*
2.0 7.5
1.5 8.5*
2.1
/3pk
AJ0-8782
AJ0-8784
AJ0-8787
AJ0-8786
AJ0-8788
Column ID (mm)
3.0
4.6
/3pk
/3pk
AJ0-8775
AJ0-8768
AJ0-8777
AJ0-8770
AJ0-8780
AJ0-8773
AJ0-8779
AJ0-8772
AJ0-8781
AJ0-8774
*pH stable 1.58.5 under gradient conditions. pH stable 1.510 under isocratic conditions.
300
Initial SecurityGuard ULTRA installation onto Kinetex columns and

later cartridge replacement requires 3 wrenches, which must be
purchased separately: one 3/8 in. wrench (AQ0-8959; fits Kinetex
column end-fitting), and two 5/16 in. wrenches (AQ0-8903; fits
ULTRA cartridge and holder). See p. 373
280
260
Cartridge Replacement
Cartridge Replacement
240
220
200
180
UHPLC / HPLC Sure-Lok High Pressure PEEK

Male Nut Fittings
With SecurityGuard ULTRA
160
140
Part No.
Description
AQ0-8503 Sure-Lok High Pressure PEEK 1-Pc Nut,
10-32, for 1/16 in. Tubing, Pressure Rated
to 12,000 psi (827 bar)
120
100
Injection No.
Protects with No Loss of Column Performance!

SecurityGuard ULTRAs unique design minimizes sample peak
dispersion to maintain column performance without altering your
chromatography results.
19860
19859
80
App ID 19859 App ID 19860
40
20
0
0.0
0.2
0.4
0.6
0.8
1.0

Sample:
Guard Cartridge: SecurityGuard Ultra C18 (ODS) 2.1 mm ID
Part No.: AJ0-8768
Phenomenex
1.2
Sure-Lok Fitting Tightening Tool, Aluminum,

for AQO-8503
Price
ea
WITHOUT SecurityGuard ULTRA

WITH SecurityGuard ULTRA
60
AQ0-8530
Unit
10/pk
HPLC | SecurityGuard ultra
No Guard
Backpressure (Bar)
n
e
w
If SecurityGuard ULTRA cartridge protection

system does not perform as well or better
than your current guard cartridge system
of similiar phase and dimensions, return
the product with comparative data within
1.4
1. Uracil
2. Acetophenone
3. Toluene
4. Naphthalene
A connectivity kit for UHPLC is available, see p. 362
UHPLC / HPLC High Pressure Stainless Steel

Male Nut Fittings
min
Part No.
Description
AQ0-8506 Nut and Ferrule Set, SS, 10-32, for 1/16 in.
Tubing, Pressure Rated to 28,000 psi (1,930 bar)
Unit
10/pk
Price
239
Selectosil
Shodex
By Showa Denko K.K.

High efficiency polymer columns
Separation Modes
Excellent mechanical and chemical stability
AFC
GPC
GFC
HIC
IC
IEC
IEX
LEX
NPC
P&A
RPC
SEC
Wide application range

High temperature GPC applications
Nomenclature of Packing Materials
Selectosil / shodex | HPLC
PHM
PMM
PS
PVA
S-DVB
polyhydroxymethacrylate
polymethylmethacrylate
polystyrene
polyvinyl alcohol
styrene-divinylbenzene co-polymer
Guide for Shodex Column Selection

Solubility
Molecular
Weight
Separation
Mode
over 2000
SEC
GPC KF-802.5-807, KD-802.5-807, KF-602.5-607
241, 242
SEC
GPC KF-801-802.5, KD-801-802.5, LF-804, KF-601-602.5
241, 242
RPC
RSpak DE-413, 413L, DM-614
245
SEC
OHpak SB-803-806HQ, SUGAR KS-803-806, PROTEIN KW-802.5-804
243
IEC
IEC QA-825, DEAE-825, SP-825, CM-825
245
AXpak WA-624
245
Waterinsoluble
Column
Page
under 2000
Sample
over 2000
HIC
HIC PH-814
245
RPC
RSpak RP18-415
245
AFpak
245
SEC
SB-802-802.5HQ, SUGAR KS-801-802
243
LEC
SUGAR SC1011, SP0810
245
IEX
RSpak KC-811, SUGAR SH1011, SUGAR SH1821
244, 245
IEC
AXpak WA-624
245
AFFINITY
Watersoluble
IC SI-90 4E, SI-50 4E, IC I-524A, Y-521, YK-421, T-521
244
RPC
RSpak DE-613, 413, DS-613
245
NPC
SUGAR SZ5532, RSpak DC-613
245
AFFINITY
AFpak
245
CHIRAL
ORpak, CDB, CDC HQ
325
IC
under 2000
240
affinity chromatography
gel permeation chromatography (organic-soluble SEC)
gel filtration chromatography (water-soluble SEC)
hydrophobic interaction chromatography
ion chromatography
ion-exchange chromatography
ion-exclusion chromatography
ligand-exchange chromatography
normal phase chromatography
partition and adsorption chromatography
reversed phase chromatography
size-exclusion chromatography (includes GPC & GFC)
Phenomenex
Shodex
By Showa Denko K.K.
Organic GPC Columns

columns packed in other solvents, such as quinoline, o-dichlorobenzene, ethyl acetate, tetrachloroethane and dimethylacetamide are available upon request.
Shodex has a wide variety of columns for GPC (or SEC) using
organic solvents. The columns are packed with porous S-DVB
gels specially developed for GPC use. Five types of GPC
columns packed in different solvents are available. Custom
In-column Solvent
THF (tetrahydrofuran)
Chloroform
DMF (dimethylformamide)
THF
HFIP (hexafluoroisopropanol)
Toluene
Toluene
THF
THF
Applications
General purpose GPC
General purpose GPC
Polar compounds such as melamine resin, phenolic resin, polyurethane and polyvinylpyrrolidone
Wide linear molecular weight
Engineering plastics such as polyamide (Nylon) and polybutylterephthalate at ordinary temperature
High temperature GPC up to 140 C
Ultra high temperature GPC up to 210 C. Polymer samples in which ultra-high molecular weight portion is included
Semi-micro GPC
Semi-micro GPC for organic polymer analysis
Calibration Curves for GPC

Mixed Bed Columns
Calibration Curves for GPC

KF-800 Series
App ID 5519
Molecular Weight (Polystyrene)
App ID 10766
Molecular Weight (Polystyrene)
102
6 7 8 9 10 11 12
Elution Volume
(ml) (mL)
Elution volume
PEEK Resin
Column:
Dimensions:
Eluent:
Flow Rate:
Detector:
Temperature:
Sample:
8 x 300 mm
THF
1.0 mL/min
UV @ 254 nm
40 C
HPLC | Shodex
6M
03
103
12
Phenol Resin
Dimensions:
Eluent:
Flow Rate:
Detector:
Temperature:
04
-8
-80
6
8
10
Elution Volume
(ml)volume (mL)
Elution
-8
KF
KF
102
104
KF
0.05 %
0.12 %
0.25 %
0.15 %
0.15 %
7L
KF KF 802
-8 -80 .5
01
2
06
105
Shodex GPC KF-802

8 x 300 mm
THF
1.0 mL/min
Shodex UV @ 254 nm
Ambient
1. PS
42,800
2. PS
2,800
3. PS
300
4. n-Propylbenzene 120
5. Benzene
78
-80
6L KF
-80
-80
-8
KF
04
5L
-8
106
-80
05
KF
103
-8
KF
KF
07
KF
105
104
-8
0
KF
10
107
-8
KF
KF
KF
Column:
Dimensions:
Eluent:
Flow Rate:
Detector:
Temperature:
Sample:
Column: Shodex GPC KF-800L series

and KF-806M
Dimensions: 8 x 300 mm
Column: Shodex GPC KF-800 series

107
Polystyrene Standards
App ID 10768
Series Name
GPC KF-800 series
GPC K-800 series
GPC KD-800 series
GPC LF-804
GPC HFIP-800 series
GPC HT-800 series
GPC UT-800 series
GPC KF-600 series
GPC KF-400HQ series
10 min
Low Density Polyethylene

Shodex GPC K-806M x 2
8 x 300 mm
CHCl3/Cl2CHCOOH=90/10
1.0 mL/min
Shodex RI
Ambient
PEEK 0.2 %, 50 L
Column: Shodex GPC UT-806M x 4,

HT-806M x 2
Eluent: 0.1 % BHT in o-Dichlorobenzene
Detector: Shodex RI
Temperature: 140 C
Sample: 1. LDPE
LF-804
(linear column)
UT - 806M X4
10
Phenomenex
20
min
30
40 min
App ID 5525
App ID 14173
KF-804L
(mixed-bed column)
App ID 5521
20
UT - 806M X2
10
15
20
25 min
241
Shodex
By Showa Denko K.K.
Organic GPC Columns (contd)

Standard Columns
Column Type / Part No.
THF
DMF
GPC KF-801
GPC KF-802
GPC KF-802.5
GPC KF-803
GPC KF-804
GPC KF-805
GPC KF-806
GPC KF-807
ID x Length
(mm)
8 x 300
8 x 300
8 x 300
8 x 300
8 x 300
8 x 300
8 x 300
8 x 300
GPC KD-801
GPC KD-802
GPC KD-802.5
GPC KD-803
GPC KD-804
GPC KD-805
GPC KD-806
GPC KD-807
Plate Number
>16,000
>16,000
>16,000
>16,000
>16,000
>10,000
>10,000
>5,000
Exclusion Limit
1.5 x 103 (KD-801, 2.5 x 103)
5 x 103
2 x 104
7 x 104
4 x 105
4 x 106
(2 x 107)
(2 x 108)
Note: 803, 804, 805, 806 and 807 are available packed in HFIP.
Mixed bed columns are specially designed to have wide linear

molecular weight ranges. These mixed bed columns are highly
recommended for correcting non-linear sections of molecular
weight calibration curves.`
Shodex | HPLC
Mixed Bed Columns

Column Type / Part No.
THF
DMF
GPC KF-803L
GPC KF-804L
GPC KF-805L
GPC KF-806M
GPC KF-807L
ID x Length
(mm)
8 x 300
8 x 300
8 x 300
8 x 300
8 x 300
8 x 300
GPC KD-806M
High Temperature GPC Columns

Column Type/Part No. ID x Length
Toluene
(mm)
GPC HT-803
8 x 300
GPC HT-804
8 x 300
GPC HT-805
8 x 300
GPC HT-806
8 x 300
Exclusion
Limit
7 x 104
4 x 105
4 x 105
(2 x 107)
Usable
Temperature (C)
100-140
100-140
100-140
100-140
Plate Number
>16,000
>16,000
>10,000
>10,000
>12,000
> 5,000
Exclusion Limit
7 x 104
4 x 105
4 x 106
(2 x 107)
(2 x 107)
(2 x 108)
Price
Note: Exclusion Limits in parentheses,( ), are estimated values.
Wide Linear Working Range

ID x Length
Column Type/Part No. (mm)
GPC LF-804
8 x 300
Downsized GPC Columns

Column Type/Part No.
THF
GPC KF-601
GPC KF-602
GPC KF-602.5
GPC KF-603
GPC KF-604
GPC KF-605
GPC KF-606
GPC KF-606M
GPC KF-607
GPC KF-G
ID x Length
(mm)
6.0 x 150
6.0 x 150
6.0 x 150
6.0 x 150
6.0 x 150
6.0 x 150
6.0 x 150
6.0 x 150
6.0 x 150
4.6 x 10
Plate
Number
> 17,000
Exclusion Limit
7 x 104
Particle Size
(m)
3
3
3
3
3
10
10
10
18
8
Price
Pore Size
()
20
60
80
100
200
500
1,000
>1,000
Plate Number
>17,000
>17,000
>17,000
>17,000
>16,000
>7,000
>7,000
>8,000
>5,000
Exclusion Limit
(Polystyrene)
1,500
5,000
20,000
70,000
400,000
4,000,000
(20,000,000)
(20,000,000)
(200,000,000)
Guard Column
Price
Notes: For all column types. Recommended flow rate: 0.4 to 0.6 mL/min; Max. usable flow rate: 0.8 mL/min; Max. usable temperature 45 C.
242
Phenomenex
Shodex
By Showa Denko K.K.
GFC (Aqueous GPC) Columns

Shodex has a wide variety of columns for GFC. Three types of
GFC columns packed with different gel materials are available.
Packing Material
PHM gel
Sulfonated PS gel
Porous silica gel
Applications
Used for general purpose GFC of water-soluble polymers, proteins and enzymes
Mono, di, tri, oligo and polysaccharides, starches and celluloses
GFC of proteins, glycoproteins and peptides
Hyaluronic Acid
Calibration Curves for OHpak SB-800HQ Series
HQ
06
4 5 6 7 8
Elution Volume (mL)
6
7
8
9
10
11
12
13
10 11 12 min
App ID 10771
Shodex SUGAR KS-802 x 2

8 x 300 mm (600 mm total length)
Water
0.3 mL/min
RI
80 C
20 L
Dietary Fiber
1. Glycerol
1
10
15
20
Aqueous GPC Columns
Column Type/
ID x Length
Part No.
(mm)
OHpak SB-802HQ
8 x 300
OHpak SB-802.5HQ
8 x 300
OHpak SB-803HQ
8 x 300
OHpak SB-804HQ
8 x 300
OHpak SB-805HQ
8 x 300
OHpak SB-806HQ
8 x 300
OHpak SB-806MHQ
8 x 300
SUGAR KS-801 (Na+)
8 x 300
SUGAR KS-802 (Na+)
8 x 300
SUGAR KS-803 (Na+)
8 x 300
SUGAR KS-804 (Na+)
8 x 300
SUGAR KS-805 (Na+)
8 x 300
SUGAR KS-806 (Na+)
8 x 300
PROTEIN KW-802.5
8 x 300
PROTEIN KW-803
8 x 300
PROTEIN KW-804
8 x 300
25
min
Plate Number
>10,000
>15,000
>15,000
>15,000
>10,000
>10,000
>10,000
>15,000
>15,000
>15,000
>15,000
>8,000
>8,000
>20,000
>20,000
>10,000
Exclusion Limit
4 x 103
1 x 104
1 x 105
1 x 106
4 x 106
(2 x 107 )
(2 x 107)
1 x 103
1 x 104
5 x 104
4 x 105
5 x 106
(5 x 107)
5 x 104
1.5 x 105
6 x 105
Price
HPLC | Shodex
Dietary Fiber
Column:
Dimensions:
Eluent:
Flow Rate:
Detector:
Temperature:
Injection Volume:
Sample:
App ID 10769
Q
MH
806
HQ
Shodex OHpak SB-805 HQ x 2

8 x 300 mm (600 mm total length)
0.1 M NaNO3
1.0 mL/min
RI
30 C
Hyaluronic Acid
SB-
02
HQ
.5
HQ
02
03
-8
-8
-8
SB
HQ
04
HQ B-8
S
05
-8
SB
SB
Molecular Weight (Pullulan)
Column:
Dimensions:
Eluent:
Flow Rate:
Detector:
Temperature:
Sample:
-8
SB

8 x 300 mm
Water
107
1. P-800
2. P-400
3. P-200
106
4. P-100
5. P-50
6. P-20
105
7. P-10
8. P-5
9. P-3
104
10. P-1
11. Maltotriose
12. Maltose
103
13. Glucose
SB
Column:
Dimensions:
Eluent:
Sample:
App ID 5527
Series Name
OHpak SB-800HQ
SUGAR KS-800
PROTEIN KW-800
Note: Exclusion Limits in parentheses,( ), are estimated values.
Calibration Standards
Calibration Standards
Standard Type/Part No.
STANDARD P-82
STANDARD SM-105
Material
Pullulan
PS
Content
0.2 g x 8 grades
0.5 g x 10 grades
MW Range
5,000 - 800,000
1,300 - 3,000,000
Applications
GFC (aqueous GPC)
GPC with THF, chloroform, toluene, etc.
Price
For full line of Polymer Calibration Standards, see p. 367
Phenomenex
243
Shodex
By Showa Denko K.K.
Columns for Organic Acids

KC-811 enables an effective organic acids separation using a mixed mode of IEX, SEC
and P&A. Organic acids also can be separated by RPC using RSpak DE-613.
RSpak
Column Type*/
ID x Length
Part No.
(mm)
RSpak KC-811
8 x 300
Plate Number
>15,000
Packing Material
S-DVB gel
Counter Ion
H+
Price
*Note: RSpak KC-811 was formerly known as Ionpak KC-811.
Ion Chromatography
Columns
Fluoride well-resolved from water dip
Shodex offers an innovative IC column for the suppressor method

that improves both the separation speed and resolution of anions
in most matrices. With high theoretical plates (>5000/m for Sulfate),
the column easily and efficiently separates organic and inorganic
anions such as EPA Method 300 analytes, acetate, formate, methacrylate and oxalate. High loading and exceptional resistance to
loading combine with features such as improved separation of the
fluoride peak from the water dip. The patent-pending PVA gel was
also specifically engineered to optimize the elution behavior of the
carbonate ion so as not to interfere with the quantitation of EPA
Method 300 anions.
Anions
Organic Acids and Inorganic Anions
Great alternative to Dionex IonPac AS4, AS4A

and AS14 columns
High efficiency, general purpose IC column
Column: SI-90 4E
Dimensions: 4.0 x 250 mm
Eluent: 1.8 mM Na2CO3
1.7 mM NaHCO3
Sample: 20 L
1. Fluoride 2 mg/L
2. Chloride 3 mg/L
3. Nitrite
5 mg/L
4. Bromide 10 mg/L
Column:
Dimensions:
Eluent:
Flow Rate:
Temperature:
Sample:
5. Nitrate
10 mg/L
6. Phosphate 15 mg/L
7. Sulfate 15 mg/L
SI-50 4E
4.0 x 250 mm
3.2 mM Na2CO3 / NaHCO3
0.7 mL/min
Ambient
20 L
1. Fluoride
2 mg/L
2. Acetate
10 mg/L
3. Formate
2 mg/L
4. Methacrylate 10 mg/L
5. Chloride
3 mg/L
6. Nitrite
5 mg/L
7. Bromide
8. Nitrate
9. Phosphate
10. Sulfate
11. Oxalate
10 mg/L
10 mg/L
15 mg/L
15 mg/L
15 mg/L
IC Columns
Column Type/
ID x Length
Part No.
(mm)
IC SI-90 4E
4.0 x 250
IC SI-90 G
4.6 x 10
IC SI-50 4E*
4.0 x 250
IC I-524A
4.6 x 100
IC Y-521
4.6 x 150
IC Y-G
4.6 x 10
IC YK-421
4.6 x 125
IC YK-G
4.6 x 10
IC T-521
4.6 x 150
IC T-G
4.6 x 10
Plate Number
>5,000 (SO4)
(Guard)
>14,000
>2,000
>3,000
(Guard)
>2,500
(Guard)
>3,000
(Guard)
Packing Material
PVA
PVA
PHM gel
Hydrophilic Polymer
S-DVB gel
App ID 14176
App ID 14175
Shodex | HPLC
Improved, high-speed separation of EPA

Method 300 analytes
Functional Group
Quaternary ammonium
Quaternary ammonium
Quaternary ammonium
Carboxyl Coated Silica
Sulfo (H+)
Applications
Inorganic anions and organic acids
(General purpose)
Inorganic anions and organic acids
Inorganic anions
Cations (general purpose)
Simultaneous separation of monovalent and divalent cations
Transition metal ions (packed in PEEK column)
Price
*Use IC SI-90G guard.
244
Phenomenex
Shodex
By Showa Denko K.K.
Columns for Proteins and Nucleic Acids

Ion-Exchange Columns
IEC series columns are suited for the analysis of proteins and nucleic acids.
AXpak WA-624 is suited for the analysis of nucleic acids.
IEC Series Columns
ID x Length
(mm)
8 x 75
8 x 75
8 x 75
8 x 75
6 x 150

IEC QA-825
IEC DEAE-825
IEC SP-825
IEC CM-825
AXpak WA-624
Plate
Number
>2,000
>2,000
>2,000
>2,000
>1,500
Packing Material
PHM gel
PHM gel
PHM gel
PHM gel
PHM gel
Functional Group
Quaternary ammonium (strong anion)
Diethylaminoethyl (weak anion)
Sulfopropyl (strong cation)
Carboxymethyl (weak cation)
Diethylaminoethyl (weak anion)
Price
Affinity Columns
ID x Length
(mm)
8 x 50
8 x 50
8 x 50
8 x 50
8 x 50
8 x 50
8 x 50
Other Columns
HIC PH-814
RSpak NN-614
ID x Length (mm)
8 x 75
6 x 150
Ligand
Concanavalin A
Cibacron Blue
Heparin
LCA (Lentil lectin)
Protein A
Protein G
Wheat germ agglutinin
Plate Number
>2,000
>5,000
Ligand Load/g
15 mg
40 mol
5 mg
7 mg
8 mg
5 mg
14 mg
Packing Material
PHM gel
PHM gel
Compound Capacity/
Column
BSA 20 mg
Lysozyme 4 mg
IgG human 20 mg
IgG human 10 mg
Functional Group
Phenyl
Applications
Glycoproteins, polysaccharides
Albumin, NAD dependent enzymes
Lipoproteins, blood coagulation factors
Human IgG, immune complexes
IgG immune complex
Separation Mode
HIC
P&A
Applications
Proteins
Cystine and cysteine
Price
Price
HPLC | Shodex

AFpak ACA-894
AFpak ACB-894
AFpak AHR-894
AFpak ALC-894
AFpak APA-894
AFpak APG-894
AFpak AWG-894
Columns for Sugar Analysis

Sugar Columns
Column Type/
ID x Length
Part No.
(mm)
SUGAR SH1011 8 x 300
SUGAR SH1821 8 x 300
SUGAR SC1011
8 x 300
SUGAR SC1821
8 x 300
SUGAR SP0810
8 x 300
SUGAR SC1211
6 x 250
SUGAR SZ5532
6 x 150
SUGAR KS-801
8 x 300
Plate
Number
>15,000
>15,000
>12,000
>12,000
>10,000
>5,000
>5,000
>15,000
Exclusion
Limit
1,000
10,000
1,000
10,000
1,000
1,000
Packing
Material
S-DVB gel
S-DVB gel
S-DVB gel
S-DVB gel
S-DVB gel
S-DVB gel
S-DVB gel
S-DVB gel
Counter
Ion
H+
H+
Ca2+
Ca2+
Pb2+
Ca2+
Zn2+
Na+
Separation
Mode
SEC + IEX
SEC + IEX
SEC + LEC
SEC + LEC
SEC + LEC
P&A + LEC
P&A + LEC
SEC + LEC
Price
Aside from the columns listed here, there are

other columns that can be used for sugar separations. Disaccharides of similar molecular
weight can be separated by NPC using RSpak
DC-613.
SUGAR KS-800 series and OHpak SB-800 HQ
series can also be used for sugar separations
by SEC.
Polymer-Based Reversed Phase Columns

RSpak
Applications
RP18
DS
500 column well-suited for protein and peptide analysis.

Suited for analysis of hydrophilic substances that cannot be retained by ODS
columns, such as medicines and food additives.
Suited for wide applications because its characteristics are similar to those of ODS
columns.
Suited for analysis of amino acids and polypeptides.
Suited for analysis of complex samples that are composed of neutral and basic
substances. Generally, neutral substances are separated by reversed phase mode,
acid substances by ion-exclusion mode and basic substances by reversed phase
mode and ion-exchange mode.
DE
DM
NN
Phenomenex
RSpak Columns
RSpak RP18-415
RSpak DS-613
RSpak DE-613
RSpak DE-413
RSpak DE-413L
RSpak DE-G (DE-613P)
RSpak DM-614
RSpak DM-G (DM-614P)
RSpak DC-613
Plate Number
>3,000
>6,000
>7,000
>11,000
>17,000
(guard column)
>4,000
(guard column)
>5,000
ID x Length (mm)
4.6 x 150
6.0 x 150
6.0 x 150
4.6 x 150
4.6 x 250
4.6 x 10
6.0 x 150
4.6 x 10
6.0 x 150
Price
245
SphereClone
If SphereClone analytical columns do not

as compared to Spherisorb columns
of the same phase, particle size and
FULL REFUND.
Guaranteed Replacement to Spherisorb

Highly reproducible
Long column life
Phenomenex SphereClone columns have been developed to

provide chromatographic behavior that mimics that of Waters
Sherisorb columns. The following page illustrates an example.
For additional comparative applications, please contact your local
Mimics performance of Waters Spherisorb

Economically priced
vs.
SphereClone
SEM of Base Silica
Spherisorb
SEM of Base Silica
SphereClone
vs.
Spherisorb
3, 5, 10 m
Particle Size
3, 5, 10 m
80
Pore Size
80
200 m /g
Surface Area
200 m2/g
Carbon Load
Sphereclone | HPLC
Surface
15,000x Magnification
Silica
6%
C6
6%
6%
C8
6%
7%
ODS(1)
6.2 %
12 %
ODS(2)
12 %
2%
NH2
2%
Surface
Physical Mass
Distribution & Shape
Physical Mass
Distribution & Shape
vs.
SphereClone 5 m Silica
Waters Spherisorb 5 m Silica
QC Data for Silica
SphereClone
Reproducibility
Batch
Dp number
Particle Diameter
Value
Pore Diameter ()
Standard Deviation
Surface Area (m2/g)

Pore Volume (mL/g)
246
5 m Particle Size Data
Dp volume
STD
Batch
Phenomenex
SphereClone
3 m Columns (mm)
Phases
150 x 2.0
Silica
C8
ODS(1)
ODS(2)
NH2
00F-4134-B0
5 m Columns (mm)
Phases
150 x 3.2
Silica
C6
C8
ODS(1)
ODS(2)
NH2
SAX
00F-4143-R0
00F-4144-R0
00F-4149-R0
10 m Columns (mm)
Phases
ODS(1)
ODS(2)
SAX
100 x 3.2
150 x 3.2
50 x 4.6
100 x 4.6
150 x 4.6
00D-4133-R0
00F-4134-R0
00F-4135-R0
00F-4137-R0
00B-4135-E0
00D-4130-E0
00D-4133-E0
00D-4134-E0
00D-4135-E0
00F-4130-E0
00F-4134-E0
00F-4135-E0
00F-4137-E0
250 x 3.2
150 x 4.6
250 x 4.6
00G-4142-R0
00G-4143-R0
00G-4144-R0
00G-4147-R0
00F-4139-E0
00F-4141-E0
00F-4142-E0
00F-4143-E0
00F-4144-E0
00F-4147-E0
00F-4149-E0
00G-4139-E0
00G-4141-E0
00G-4142-E0
00G-4143-E0
00G-4144-E0
00G-4147-E0
00G-4149-E0

4 x 2.0
4 x 3.0
/10pk
/10pk
AJ0-4347
AJ0-4348
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
AJ0-4286
AJ0-4287
AJ0-4301
AJ0-4302
AJ0-4310
AJ0-4311
for ID: 2.0-3.0 mm
3.2-8.0 mm

4 x 3.0
/10pk
AJ0-4287
AJ0-4287
AJ0-4311
for ID: 3.2-8.0 mm
250 x 4.6
00G-4155-E0
00G-4156-E0
00G-4160-E0

4 x 2.0
4 x 3.0
/10pk
/10pk
AJ0-4347
AJ0-4348
AJ0-4289
AJ0-4290
AJ0-4286
AJ0-4287
AJ0-4286
AJ0-4287
AJ0-4301
AJ0-4302
for ID: 2.0-3.0 mm 3.2-8.0 mm

SphereClone is a trademark of Phenomenex.
+
Spherisorb columns used for comparison studies were purchased from manufacturer.
Spherisorb is a registered trademark of Waters. Phenomenex has no affiliation with Waters.
Spherisorb
Expertly packed by Phenomenex

Phenomenex packs Spherisorb material to high standards of
quality. We have been packing Spherisorb columns for over 25
years and frequently get requests from customers who have
to use Spherisorb and want Phenomenex-packed Spherisorb
instead.
Use SphereClone Columns

as an Alternative to Spherisorb
See p. 246
HPLC | Sphereclone / Spherex / Spherisorb
Spherex
Ordering Information SecurityGuard Analytical Cartridges require universal holder Part No.: KJ0-4282
5 m Columns (mm)
Phase
C8
ODS(1)
ODS(2)
150 x 4.6
00F-0104-E0
00F-0105-E0
00F-0106-E0

250 x 4.6
4 x 3.0
/10pk
00G-0104-E0
AJ0-4290
00G-0105-E0
AJ0-4287
00G-0106-E0
AJ0-4287
for ID: 3.2-8.0 mm
Spherisorb is a registered trademark of Waters Corp.

Phenomenex is not affiliated with Waters Corp.
Phenomenex
247
Star-Ion A300
If STAR-ION A300 does not produce

at least equivalent separation as compared to a competing column of a similar
type*, simply send us comparative data
with the STAR-ION A300 column within
45 days for a full refund.
Suppressed Mode Anion Analysis

for EPA Method 300
Excellent separation of inorganic anions
and some common organic anions
High resolution and peak symmetry
An alternative to Dionex IonPac AS4A
Material Specifications
Material Type
Mode of IC
Max. Temperature
Max. Pressure
PSDVB with quaternary amine functionality

Suppressed (optimized)
45 C
1000 psi without guard column
1200 psi with guard column
Solvent Limitations
No organic solvents are recommended

for use with STAR-ION
EPA Method 300
Halogen Anions
Column:
Dimensions:
Part No.:
Flow Rate:
Detection:
Pressure:
Injection Volume:
Eluent:
Sample:
STAR-ION A300
100 x 4.6 mm
00D-4090-E0-BV
2.0 mL/min
1.7 mM NaHCO3/ 1.8 mM Na2CO3
Suppressed Conductivity
20 L
1. Fluoride
2 mg/L
2. Chloride 20 mg/L
3. Nitrite
2 mg/L
4. Bromide
2 mg/L
5. Nitrate
10 mg/L
6. Phosphate 2 mg/L
7. Sulfate
60 mg/L
Volts
STAR-ION A300
100 x 4.6 mm
00D-4090-E0-BV
1.5 mL/min
620 psi
20 L
3.6 mM Na2CO3
1. Fluoride 10 ppm
2. Chloride 20 ppm
3. Bromide 20 ppm
4. Bromate 20 ppm
5. Chlorate 20 ppm
6. Iodide 20 ppm
0.10
0.05
2
0.08
7
0.04
0.06
0.03
0.02
App ID 5604
0.04
5
3
0.00
0.02
5
6
0.01
App ID 5613
Star-Ion | HPLC
Column:
Dimensions:
Part No.:
Flow Rate:
Eluent:
Detection:
Injection Volume:
Sample:
0.00
0
10
min
Suppressed Mode Anion Analysis for EPA Method 300
Part No.
Description
00D-4090-E0-BV
STAR-ION A300 Anion column (PEEK)
AQ0-3111
STAR-ION Guard cartridge holder with 2 PEEK long-nut fittings
KH0-3112
STAR-ION Anion guard cartridge
AL0-3420
STAR-ION A300 Test Mix
AQ0-3351
PEEK in. - 28 to 10-32 Adapter to connect STAR-ION A300
analytical column to Dionex IC systems
(use 2 fittings, one for each end of column)
AQ0-1388
Replacement PEEK long-nut fitting
AT0-1107
PEEK capillary tubing 1/16 in. OD x 0.010 in. D x 5 ft. L
AT0-1110
Polymer tubing cutter
10
Dimensions (mm)
100 x 4.6
10 x 4.6
Unit
ea
ea
3/pk
ea
ea
min
Price
ea
ea
ea
* Suppressed mode ion columns.

IonPac AS4A and Dionex are trademarks of Dionex Corp. Phenomenex has no affiliation with Dionex.
248
Phenomenex
Synergi
Full Range Selectivity for Reversed Phase Separation

Many different mechanisms of retention are utilized within reversed phase chromatography in order to retain and
separate target analytes. Whether your compounds are hydrophobic or polar, Synergi columns provide you with a full
range of selectivity, ensuring separation of the most challenging and complex mixtures.
Synergi Polar-RP
Phenyl Ether-Linked
Synergi Fusion-RP
C18 Polar Embedded
For polar and aromatic mixtures
Balanced non-polar and polar performance

Embedded polar group
complements C18 ligand with
balanced polar selectivity
Ether linkage increases aromaticity

of the phenyl group and also provides -
interactions with conjugated compounds
TMS endcapping
ensures sharp peaks
Polar endcapping provides added

retention for polar compounds
Aq
Aq
Aq
Aq
TMS
SFC
TMS
Ultra-pure Silica
Ultra-pure Silica
Synergi Max-RP
C12 TMS Endcapped
Strong non-polar and polar retention
Excellent for basic compounds at neutral pH
Polar endcapping provides added

retention for polar compounds
HPLC | Synergi
Synergi Hydro-RP
C18 Polar Endcapped
Aq
APPROVED
High density ligands and extensive

endcapping ensure sharp peaks
Aq
TMS
Ultra-pure Silica
TMS
Ultra-pure Silica
Packing Material
Particle
Shape/Size
(m)
Pore
Size
()
Pore
Volume
(mL/g)
Surface
Area
(m2/g)
Carbon
Load
%
Calculated
Bonded Phase
Coverage
(mole/m2)
End
Capping
Synergi Max-RP
Synergi Hydro-RP
Synergi Polar-RP
Synergi Fusion-RP
Synergi Max-RP
Synergi Hydro-RP
Synergi Polar-RP
Synergi Fusion-RP
Spher. 2.5
Spher. 2.5
Spher. 2.5
Spher. 2.5
Spher. 4, 10
Spher. 4, 10
Spher. 4, 10
Spher. 4, 10
100
100
100
100
80
80
80
80
1.05
1.05
1.05
1.05
400
400
400
400
475
475
475
475
17
19
11
12
17
19
11
12
3.21
2.45
3.15
N/A
TMS
Hydrophilic
Hydrophilic
TMS
TMS
Hydrophilic
Hydrophilic
TMS
Phenomenex
249
Synergi
If Synergi analytical columns do not

similar particle size, similar phase and
FULL REFUND.
Synergi Polar-RP
An Ether-linked Phenyl Column with
Polar Endcapping
Synergi Polar-RP
USP: L11
LC/MS
Certified
pH Stability:
Particle Size:
Phase:
Application:
1.5 7.0
2.5 m, 4 m, and 10 m
Ether-linked phenyl with polar endcapping
For extreme retention of polar and aromatic
compounds
Strength: Improved peak shape for acidic and basic
analytes and aromatic selectivity with
methanol containing mobile phases
Ether-Linked Phenyl with Polar Endcapping

Synergi Polar-RP
Sample Challenge:
SFC
APPROVED
You need greater separation between polar and aromatic

compounds with only slight differences chemically or structurally.
Selectivity Solution:
Increased resolution of polar compounds with Synergi Polar-RP

compared to traditional C18 phases
Improve peak symmetry of polar compounds with Synergi Polar-RP compared to other phenyl phases
Waters 5 m
XTerra RP18*
12525
Waters 5 m
SymmetryShield RPC18*
Famotidine Peak
GL Sciences
Inertsil 5 m
Phenyl PH-3*
Columns:
Dimensions:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Injection:
Sample:
App ID 12527
Columns:
Waters 5 m
Symmetry C18*
Synergi 4 m Polar-RP
ZORBAX 5 m SB-Phenyl*
12526
12485
Improved Selectivity!
Synergi
| HPLC
The slightest variations in polarity and aromaticity are exploited

by Synergi Polar-RP in order to achieve the greatest separation
between polar and/or aromatic compounds.
Dimensions:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:
ZORBAX 5 m SB-Phenyl
Inertsil 5 m Phenyl PH-3
150 x 4.6 mm
20 mM Potassium phosphate pH 7.0/Acetonitrile (80:20)
1.0 mL/ min
UV @ 254 nm
25 C
Famotidine
Waters 5 m SymmetryShield RPC18
Waters 5 m Symmetry C18
Waters 5 m XTerra RP18
150 x 4.6 mm
20 mM Potassium phosphate pH 3 / Methanol (50:50)
1.0 mL/ min
UV @ 230 nm
Ambient
2 L
1. Metaproterenol (0.4 g)
4. Alprenolol (0.3 g)
2. Pindolol (0.6 g)
5. Propranolol (0.04 g)
3. Metoprolol (0.15 g)
6. Ethylparaben (0.4 g)
*See p. 253 for disclaimer information. Comparative separations may not be representative of all applications.
250
Phenomenex
Synergi
Synergi Polar-RP (cont'd)

The selectivity of Synergi Polar-RP can provide differences in peak elution
order for confirmation or better separation
Synergi Polar-RP is highly reproducible
Alternative selectivity
App ID 14236
Reproducible Performance
Synergi 4 m Hydro-RP
SFC

Mobile Phase: A: 20 mM KH2PO4 + 0.1 % Hexane Sulfonate, pH 3.0
B: Acetonitrile
Gradient: A/B (97:3) for 3 minutes, then to
A/B (50:50) in 15 minutes
Flow Rate: 1.5 mL/ min
Temperature: 22 C
Sample: 1. Pantothenic acid
2. Pyridoxine
3. p-Aminobenzoic acid
4. Thiamine
5. Riboflavin

Column: Synergi 4 m Polar-RP
Part No.: 00F-4336-E0
Mobile Phase: Methanol/20 mM Potassium
Phosphate pH 6.5 (35:65)
Injection: 1 L
Temperature: 22 C
Sample: 1. Phenylephrine (1g)
2. Phenylpropanolamine (1g)
3. Pseudoephedrine (1g)
4. Methylparaben (1g)
HPLC | Synergi
App ID 14235
App ID 12471
APPROVED
100 % buffer mobile phase stability allows for separation of extremely polar compounds, like nucleic acid bases, on Synergi Polar-RP
Resolution under
100 % buffer conditions
App ID 12487
Nucleic Acid Bases
Phenomenex
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Injection:
Temperature:
Sample:
150 x 4.6 mm
00F-4336-E0
20 mM Potassium phosphate pH 2.7
2.0 mL/ min
UV @ 254 nm
2.5 L
22 C
1. Thymidine triphosphate (TTP) (1.25 g)
2. Thymidine diphosphate (TDP) (1.25 g)
3. Thymidine monophosphate (TMP) (1.25 g)
4. Thymidine (1.25 g)
251
Synergi

FULL REFUND.
Synergi Fusion-RP
A Polar Embedded C18 Column
C18
C18
C18
Synergi Fusion-RP
C18
LC/MS
Certified
USP: L1
pH Stability:
Particle Size:
Phase:
Application:
1.5 9.0*
2.5 m, 4 m, and 10 m
Polar embedded C18
For a balanced retention of polar, basic compounds
and moderate retention of hydrophobics over a broad
pH range
Strength: Analysis of polar, basic compounds with little or no
MS phase bleed
Polar
Group
C18 with Polar Embedding

Synergi Fusion-RP
* pH range is 1.5-10.0 under isocratic conditions. pH range is 1.5-9 under gradient conditions.
NO-NITROGEN Polar Infused C18 Column
Sample Challenge:
SFC
APPROVED
| HPLC
Offering a balanced combination of hydrophobic and polar

selectivity, Synergi Fusion-RP will allow you to separate
compounds exhibiting polar and hydrophobic characteristics.
Balanced Polar and Hydrophobic Retention Allows for Superior Selectivity
14839
Longer Polar
Retention
1
2
Antihistamines
Synergi 4 m
Fusion-RP
Less Hydrophobic
Retention
3
4
10
12
2
2
3
4
10
14
min
Columns: Synergi 4 m Fusion-RP

Typical C18
Mobile Phase: 20 mm Potassium Phosphate,
pH 2.5 / Acetonitrile (75:25)
Sample: 1. Maleic acid
2. Chlorpheniramine
3. Triprolidine
4. Diphenhydramine
min
Waters 5 m
SymmetryShield RPC18
3
4
min
Supelco Discovery
5 m RP-AmideC16
2&3
1
12
4
6
min
Typical C18
14
Synergi 4 m
Fusion-RP
App ID 14839
Hydrophobic Basic Compounds
App ID 14840
Synergi
840
You need greater separation of compounds that exhibit

moderately polar and hydrophobic characteristics.
min
Columns: Synergi 4 m Fusion-RP

Waters 5 m SymmetryShield RPC18
Supelco Discovery 5 m RP-AmideC16
Mobile Phase: 20 mm Potassium Phosphate, pH 2.5 /
Methanol (70:30)
Sample: 1. Phenylephrine
2. Phenylpropanolamine
3. Pseudoephedrine
252
Phenomenex
Synergi
Synergi Fusion-RP (contd)
14891
100 % aqueous stable for added method flexibility
Synergi Fusion-RP has negligible MS bleed compared to other

polar modified C18 columns

App ID 14891
At time=0
x107
TIC: Total Ion Current
Waters 5 m
SymmetryShield RPC18
0.5
0.4
0.2
Synergi 4 m
Fusion-RP
0.3
0.6
10
12
1 4 min
After 24 hours of 100 %

Aqueous Conditions
2
10
0.1
x106
min

Mobile Phase: A: 0.1 % CH3COOH in Water
B: 0.1 % CH3COOH in Methanol
Gradient: 95:5 (A/B) linear to 5:95 over 8 min, hold
for 5 min
Detection: Bruker-Daltonics Esquire 2000 IT
Ion Source: ESI
Scan Rate: 13000 m/z/s
Scan Range: 50-1000
Column:
Dimensions:
Mobile Phase:
Flow Rate:
Detection:
Injection:
Temperature:
Sample:
12
1 4 min
Synergi 4 m Fusion-RP
150 x 4.6 mm
20 mM Potassium phosphate buffer pH 2.5
1.0 mL/ min
UV @ 254 nm
1 L
30 C
1. Thiourea
2. Adenine
3. Guanosine-5-monophosphate
4. Thymine
SFC
APPROVED
Achieve reproducibility and long column lifetimes even at pH extremes with Synergi Fusion-RP
pH 10.0
9
11000
10000
7
9000
8000
7000
6000
tR of toluene (min.)
K of toluene
5000
symmetry of toluene
symmetry of pyridine
4000
N of toluene (plates/column)
3000
2000
1
0
14000
12000
1000
0
0
1000
2000
Column Volumes
3000
4000
13000
12000
11000
10000
9000
HPLC | Synergi
pH 1.5
9
8000
5
tR of toluene (min.)
7000
K of toluene
6000
symmetry of toluene
symmetry of pyridine
5000
N of toluene (plates/column)
3000
4000
2000
1000
0
0
1000
2000
3000
4000
5000
6000
7000
8000
9000
10000
Column Volumes
pH testing was done under isocratic conditions with phosphate buffer. Formic acid and ammonium formate were also used as test buffers.
*Comparative separations may not be representative of all applications.

Luna and Phenomenex are registered trademarks of Phenomenex, Inc.
Synergi is a registered trademark of Phenomenex, Inc.
Discovery is a registered trademark of Sigma-Aldrich Biotechnology. Phenomenex has no affiliation
with Sigma-Aldrich. Columns used for comparison studies were manufactured by and purchased from
Sigma-Aldrich.
Nucleosil is a registered trademark of Macherey Nagel. Phenomenex has no affiliation with Macherey
Nagel. Columns used for comparison studies were manufactured by and purchased from Macherey
Nagel.
Inertsil is a registered trademark of GL Sciences Inc.. Phenomenex has no affiliation with GL Sciences
Inc. Columns used for comparison studies were manufactured by and purchased from GL Sciences Inc.
SymmetryShield is a trademark of Waters Corp. XTerra and Symmetry are registered trademarks of
Waters Corp. Phenomenex has no affiliation with Waters Corp. Columns used for comparison studies
were manufactured by and purchased from Waters Corp.
Zorbax SB and Zorbax Eclipse XDB are registered trademarks of Agilent Technologies. Phenomenex
has no affiliation with Agilent Technologies, Inc. Columns used for comparison studies were
manufactured by and purchased from Agilent Technologies.
Phenomenex
253
Synergi

FULL REFUND.
Synergi Hydro-RP
A Polar Endcapped C18 Column
Synergi Hydro-RP
USP: L1
pH Stability:
Particle Size:
Phase:
Application:
1.5 7.5
2.5 m, 4 m, and 10 m
C18 with polar endcapping
For extreme retention of non-polar and extremely
polar alkyl compounds
Strength: Resolution of highly polar compounds under 100 %
buffer mobile phase conditions
C18 with Polar Endcapping

Synergi Hydro-RP
Sample Challenge:
SFC
APPROVED
Your sample contains multiple analytes with only slight variations

in hydrophobicity.
Synergi
| HPLC
The extreme hydrophobic selectivity offered by Synergi Hydro-RP

is needed to amplify the small differences in selectivity and get
greater separation.
Extreme hydrophobic retention relative to other hydrophobic

selectivity phases
Additional polar selectivity provides separation where traditional C18

columns cannot
Synergi 4 m Hydro-RP 80
App ID 14425

Mobile Phase: Acetonitrile/20 mM
Potassium phosphate
pH 7.0 (65:35)
Sample: 1. Butylbenzene
2. Amylbenzene
254
The chart was obtained by plotting

hydrophobic retention (k for
butylbenzene vs. methylene selectivity
(log k for amylbenzene vs the number
of methyl groups) under the stated
conditions. A column with high
hydrophobicity will better resolve two
analytes which subtly differ in their
overall hydrophobicity than a column
with lower hydrophobic selectivity.
App ID 14424
Luna 5 m C18(2) 100

Mobile Phase: 20 mM Potassium phosphate,
pH 7.0 /Methanol (60:40)
Flow Rate: 1 mL/ min
Temperature: 22 C
Sample: 1. Phenylephrine
2. Phenylpropanolamine
3. Pseudoephedrine
4. Methylparaben
Phenomenex
Synergi
Synergi Hydro-RP (contd)

Synergi Hydro-RP is stable in 100 % aqueous mobile phase, providing improved retention of extremely polar compounds
Aqueous stable for over

7000 mL of buffer
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Injection:
Sample:
150 x 4.6 mm
00F-4375-E0
20 mM Potassium phosphate, pH 2.5
1.0 mL/min
35 C
UV @ 210 nm
5 L
1. Norepinephrine (0.8 mg/mL)
2. Epinephrine (0.5 mg/mL)
3. Normetanephrine (0.6 mg/mL)
4.Dopamine (0.4 mg/mL)
5 L-DOPA (0.3 mg/mL)
SFC
Synergi Hydro-RP is able to separate very polar compounds, like organic acids and catecholamines, under 100 % aqueous conditions
APPROVED
Polar retention under

100% aqueous conditions
10
Organic Acids
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:
Phenomenex
250 x 4.6 mm
00G-4375-E0
20 mM Potassium
phosphate, pH 2.9
0.7 mL/min
22 C
UV @ 220 nm
1. Oxalic acid
2. Tartaric acid
3. Glycolic acid
4. Formic acid
5. Pyruvic acid
6. Malonic acid
7. Acetic acid
8 Maleic acid
9. Citric acid
12
14
App ID 14217
App ID 14270
HPLC | Synergi
Polar retention under

100% aqueous conditions
min
Catecholamines
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:
150 x 4.6 mm
00F-4375-E0
20 mM Potassium phosphate, pH 2.5
1 mL/min
22 C
UV @ 210 nm
1. Norepinephrine
2. Epinephrine
3. 6-Hydroxydopamine
4. Normetanephrine
5. Dopamine
6. L-DOPA
7. Epinine
255
Synergi

FULL REFUND.
Synergi Max-RP
A Reversed Phase C12 Column
C12
C12
Synergi Max-RP
C12
C12
LC/MS
Certified
pH Stability:
Particle Size:
Phase:
Application:
1.5 9.0*
2.5 m, 4 m, and 10 m
Reversed phase C12
For hydrophobic, non-polar compounds over a
wide pH range, with little or no MS phase bleed
Strength: Sharp peak shape for basic compounds
at neutral pH
C12 with TMS Endcapping

Synergi Max-RP
* pH range is 1.5-10.0 under isocratic conditions. pH range is 1.5-9 under gradient conditions.
Sample Challenge:
SFC
APPROVED
You need to retain compounds based on hydrophobic selectivity

exclusively, but cannot accomplish peak separation with C18
column.
Synergi
| HPLC
The C12 ligands on Synergi Max-RP give a hydrophobic

selectivity that may separate peaks where C18 columns cannot.
Silanol Activity at Low pH: C12 vs. C18 Phases
Hydrophobic Retention:
Synergi Max-RP (C12) Performs Like a C18*
0.100
10.000
9.110
9.000
alpha of benzylamine/phenol
8.280
8.000
k butylbenzene
7.000
6.370
6.740
0.095
0.088
0.090
8.570
6.930
6.000
5.000
4.000
0.079
0.080
0.070
0.059
0.060
0.050
0.060
0.048
0.040
0.030
3.000
0.020
2.000
0.010
1.000
0.000
0.000
Synergi 4 m Max-RP
XTerra 5 m MS C18
ZORBAX 5 m SB C18 Nucleosil 5 m C18
Luna 5 m C18(2)
XTerra 5 m MS C18 ZORBAX 5 m SB C18 ZORBAX 5 m XDB C18 Nucleosil 5 m C18
Synergi 4 m Max-RP ZORBAX 5 m XDB C18 Luna 5 m C18(2)
Columns: Waters XTerra 5 m MS C18

Agilent Technologies ZORBAX 5 m SB C18
Macherey Nagel Nucleosil 5 m C18
Synergi 4 m Max-RP
Agilent Technologies ZORBAX 5 m XDB C18
Luna 5 m C18(2)
Injection: 1 L
Sample: Butylbenzene
Columns: Waters XTerra 5 m MS C18

Agilent Technologies ZORBAX 5 m SB C18
Macherey Nagel Nucleosil 5 m C18
Synergi 4 m Max-RP
Agilent Technologies ZORBAX 5 m XDB C18
Luna 5 m C18(2)
Mobile Phase: Methanol/20 mM
Potassium phosphate,
pH 2.5 (30:70)
Injection: 5 L
Sample: 1. Benzylamine
2. Phenol
256
Phenomenex
Synergi
Synergi Max-RP (cont'd)

Sharper Peaks
The Synergi Max-RP C12 ligands are densely bound to silica surface, significantly decreasing the number of active silanol
groups, which cause peak tailing
3.5 m Eclipse XDB-C18
App ID 12519
App ID 10896
Synergi 4 m Max-RP
Peak Asymmetry = 1.25
WatersXTerra MS 5 m C18
App ID 12476
Synergi 4 m Max-RP
Peak Asymmetry = 1.58
Columns: Synergi 4 m Max-RP

Waters XTerra MS 5 m C18
Mobile Phase: 20 mM Potassium phosphate pH 7 / Acetonitrile /
Methanol (50:25:25)
Injection: 1 L
Temperature: 30 C
Sample: Methanol extract from Chlortrimeton Allergy Pills
1. Chlorpheniramine
SFC
APPROVED
HPLC | Synergi
Columns: Synergi 4 m Max-RP

Agilent Technologies ZORBAX 3.5 m Eclipse XDB-C18
Mobile Phase: A: Water with 0.1 % Formic acid
B: Acetonitrile with 0.1 % Formic acid
Gradient: A/B (95:5) to 100 % B in 5 min
Injection: 5 L
Temperature: 30 C
Sample: 1. Thiourea (0.25 g)
2. Codeine (1 g)
3. Chlorpheniramine (2 g)
4. Propranolol (6 g)
5. Desipramine (0.5 g)
6. Ibuprofen (6 g)
Achieve Reproducibility and Long Column Lifetimes Even at pH Extremes with Synergi Max-RP
Stability @ pH 1.5
Stability @ pH 10.0
Over 18,000 Column
Volumes of Mobile Phase!
Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Injection:
Temperature:
Sample:
Phenomenex
Synergi 4 m Max-RP
30 x 2.0 mm
00A-4337-B0
A: Water with 0.1 % TFA
B: Acetonitrile with 0.1 % TFA
A/B (95:5) to
A/B (5:95) in 3 min
1.0 mL/ min
UV @ 254 nm
1 L
30 C
Precipitated porcine serum
(2:1 Acetonitrile:serum)
containing:
1. Oxazepam (50 ng)
2. Temazepam (50 ng)
3. Nordiazepam (50 ng)
4. Diazepam (50 ng)
App ID 12482
App ID 12481
Over 18,000 Column

Volumes of Mobile Phase!
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Detection:
Injection:
Temperature:
Sample:
Synergi 4 m Max-RP
30 x 2.0 mm
00A-4337-B0
Water with 0.1 % Ammonium hydroxide, pH 10 /
Acetonitrile with 0.1 % Ammonium hydroxide,
pH 10 (50:50)
0.5 mL/ min
UV @ 254 nm
5 L
30 C
1. Pyridine
2. Phenol
3. Toluene
257
Synergi
If Synergi analytical columns do

not provide at least an equivalent
separation as compared to a competing
column of similar particle size, similar
phase and dimensions, return the
column with comparative data within
Fast LC Solutions
2.5 m High Speed Technology (HST) Columns (mm)
Phases
30 x 2.0
50 x 2.0
Max-RP
Hydro-RP
Polar-RP
Fusion-RP
00A-4372-B0
00A-4387-B0
00A-4371-B0
00A-4423-B0
00B-4372-B0
00B-4387-B0
00B-4371-B0
00B-4423-B0
100 x 2.0
50 x 3.0
100 x 3.0
00D-4372-B0
00D-4387-B0
00D-4371-B0
00D-4423-B0
00B-4372-Y0
00B-4387-Y0
00B-4371-Y0
00B-4423-Y0
00D-4372-Y0
00D-4387-Y0
00D-4371-Y0
00D-4423-Y0
50 x 4.6
00B-4372-E0
00B-4387-E0
00B-4371-E0
00B-4423-E0
For information about HST Columns, contact your Phenomenex technical consultant or local distributor.
SFC
APPROVED
2.5 m MercuryMS LC/MS Cartridges (mm)
Phases
10 x 2.0
10 x 4.0

1 @ 250 /ea

1 @ 250 /ea

3 @ 240 /ea

3 @ 240 /ea
Qty Pricing

5 @ 225 /ea

5 @ 225 /ea
10 @ 215 /ea
10 @ 215 /ea
Max-RP
00N-4372-B0-CE
00N-4372-D0-CE
Hydro-RP
00N-4387-B0-CE
00N-4387-D0-CE
Polar-RP
00N-4371-D0-CE
Fusion-RP
00N-4423-B0-CE
00N-4423-D0-CE
20 x 2.0
1 @ 250 /ea
3 @ 240 /ea
5 @ 225 /ea
10 @ 215 /ea
00M-4372-B0-CE
00M-4387-B0-CE
00M-4371-B0-CE
00M-4423-B0-CE
Columns (mm)
20 x 2.0
20 x 4.0
00M-4372-B0
00M-4387-B0
00M-4371-B0
00M-4423-B0
00M-4372-D0
00M-4423-D0
MercuryMS Screening Kits

These convenient screening kits allow quick,
easy and economical evaluation of multiple
phases. Each kit contains one cartridge of
each available phase and a standard holder.
Available in either 3 m or 5 m Luna with
2.5 m Synergi.
Direct-Connect Holder
Synergi
| HPLC
MercuryMS Cartridge Holders
20 x 4.0
1 @ 250 /ea
3 @ 240 /ea
5 @ 225 /ea
10 @ 215 /ea
00M-4372-D0-CE
00M-4387-D0-CE
00M-4371-D0-CE
00M-4423-D0-CE
Standard Holder
Screening Kits
Direct-Connect Cartridge Holders
Part No.
Description
CH0-7187
CH0-7188
Price
Standard Cartridge Holders

Part No.
Description
CH0-5846
CH0-5845
Kit A includes 1 ea of:

Kit B includes 1 ea of:
Price
Capillary Columns
4 m Synergi Capillary Columns (mm)
Phases
50 x 0.30
150 x 0.30
Max-RP
Hydro-RP
Fusion-RP
00B-4337-AC
00B-4375-AC
00B-4424-AC
00F-4337-AC
00F-4375-AC
00F-4424-AC
50 x 0.50
150 x 0.50
250 x 0.50
Guard Columns (mm)

20 x 0.30
00B-4375-AF
00B-4424-AF
00F-4375-AF
00F-4424-AF
00G-4337-AF
00G-4375-AF
00G-4424-AF
03M-4375-AC
03M-4424-AC
20 x 2.0 mm Kit
KH0-7333
20 x 4.0 mm Kit
KH0-7334
KH0-7335
KH0-7336
For Fused Silica Capillary Adapter, see p. 360
258
Phenomenex
Synergi

FULL REFUND.
HPLC Columns
4 m Microbore and Minibore Columns (mm)
Phases
50 x 1.0
150 x 1.0
Max-RP
Hydro-RP
Polar-RP
Fusion-RP
00B-4337-A0
00B-4375-A0
00B-4336-A0
00B-4424-A0
00F-4337-A0
00F-4375-A0
00F-4336-A0
00F-4424-A0
4 m Narrow Bore Columns (mm)

Phases
30 x 3.0
Max-RP
Hydro-RP
Polar-RP
Fusion-RP
00A-4337-Y0
00A-4375-Y0
00A-4336-Y0
00A-4424-Y0

Phases
Max-RP
Hydro-RP
Polar-RP
Fusion-RP
250 x 1.0
30 x 2.0
50 x 2.0
75 x 2.0
150 x 2.0
00G-4375-A0
00A-4337-B0
00A-4375-B0
00A-4336-B0
00A-4424-B0
00B-4337-B0
00B-4375-B0
00B-4336-B0
00B-4424-B0
00C-4337-B0
00C-4375-B0
00C-4336-B0
00C-4424-B0
00F-4337-B0
00F-4375-B0
00F-4336-B0
00F-4424-B0
50 x 3.0
150 x 3.0
250 x 3.0
00B-4337-Y0
00B-4375-Y0
00B-4336-Y0
00B-4424-Y0
00F-4337-Y0
00F-4375-Y0
00F-4336-Y0
00F-4424-Y0
00G-4337-Y0
00G-4375-Y0
00G-4336-Y0
00G-4424-Y0
30 x 4.6
50 x 4.6
75 x 4.6
150 x 4.6
00A-4337-E0
00A-4375-E0
00A-4336-E0
00A-4424-E0
00B-4337-E0
00B-4375-E0
00B-4336-E0
00B-4424-E0
00C-4337-E0
00C-4375-E0
00C-4336-E0
00C-4424-E0
00F-4337-E0
00F-4375-E0
00F-4336-E0
00F-4424-E0

4 x 3.0*
/10pk
00G-4337-E0
AJ0-6074
00G-4375-E0
AJ0-7511
00G-4336-E0
AJ0-6076
00G-4424-E0
AJ0-7557
for ID: 3.2-8.0 mm
250 x 4.6
150 x 21.2
250 x 21.2
00F-4337-P0-AX
00F-4375-P0-AX
00F-4336-P0-AX
00F-4424-P0-AX
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
00G-4337-P0-AX
00G-4375-P0-AX
00G-4336-P0-AX
00G-4424-P0-AX
Axia Packed Preparative Columns (mm) continued

Phases
50 x 30
75 x 30
100 x 30
4 m
Max-RP
00B-4337-U0-AX 00C-4337-U0-AX 00D-4337-U0-AX
Hydro-RP
00B-4375-U0-AX 00C-4375-U0-AX 00D-4375-U0-AX
Polar-RP
00B-4336-U0-AX 00C-4336-U0-AX 00D-4336-U0-AX
Fusion-RP
00D-4424-U0-AX
10 m
Max-RP
00B-4350-U0-AX
00D-4350-U0-AX
Hydro-RP
00B-4376-U0-AX
Polar-RP
00B-4351-U0-AX
Fusion-RP
00D-4425-U0-AX
00G-4350-P0-AX
00G-4376-P0-AX
00G-4351-P0-AX
00G-4425-P0-AX
250 x 30
aufInquire
Anfrage
00G-4337-U0-AX
00G-4375-U0-AX
00G-4336-U0-AX
00G-4424-U0-AX
aufInquire
Anfrage
00G-4350-U0-AX
00G-4376-U0-AX
00G-4351-U0-AX
SecurityGuard
Cartridges (mm)
15 x 21.2**
/ea
AJ0-7842
AJ0-7843
AJ0-7845
AJ0-7844
/ea
AJ0-7842
AJ0-7843
AJ0-7845
AJ0-7844
for ID: 18-29 mm
SecurityGuard
Cartridges (mm)
15 x 30.0
/ea
AJ0-8304
AJ0-8305
AJ0-8307
AJ0-8306
/ea
AJ0-8304
AJ0-8305
AJ0-8307
AJ0-8306
for ID: 30-49 mm
Pilot Scale Columns and Bulk Material

SecurityGuard
Cartridges (mm
4 x 3.0*
10 x 10
/10pk
/3pk
AJ0-6074
AJ0-7275
AJ0-7511
AJ0-7512
AJ0-6076
AJ0-7276
AJ0-7557
AJ0-7558
for ID: 3.2-8.0 mm
9-16 mm
10 m Analytical and Semi-Prep Columns (mm)

Phases
250 x 4.6
250 x 10
Phenomenex
00G-4350-N0
00G-4376-N0
00G-4351-N0
00G-4425-N0
1 kg
aufInquire
Anfrage
04K-4350
04K-4376
04K-4351
04K-4425
5 kg
aufInquire
Anfrage
04L-4350
04L-4376
04L-4351
04L-4425

Phases
250 x 10
Max-RP
Hydro-RP
Polar-RP
Fusion-RP
00G-4337-N0
00G-4375-N0
00G-4336-N0
00G-4424-N0
SecurityGuard
Cartridges (mm)
10 x 10
/3pk
AJ0-7275
AJ0-7512
AJ0-7276
AJ0-7558
for ID: 9-16 mm

HPLC | Synergi

Phases
50 x 21.2
100 x 21.2
4 m
Max-RP
00B-4337-P0-AX 00D-4337-P0-AX
Hydro-RP
00B-4375-P0-AX 00D-4375-P0-AX
Polar-RP
00B-4336-P0-AX 00D-4336-P0-AX
Fusion-RP
00B-4424-P0-AX 00D-4424-P0-AX
10 m
Max-RP
00D-4350-P0-AX
Hydro-RP
Polar-RP
Fusion-RP
10 m Bulk Packings
Phases
100 g
aufInquire
Anfrage
Max-RP
04G-4350
Hydro-RP
04G-4376
Polar-RP
04G-4351
Fusion-RP
04G-4425
SFC
APPROVED
00G-4350-E0
00G-4376-E0
00G-4351-E0
00G-4425-E0
for ID: 2.0-3.0 mm

4 x 2.0*
/10pk
AJ0-6073
AJ0-7510
AJ0-6075
AJ0-7556
for ID: 2.0-3.0 mm
Preparative Columns
Max-RP
Hydro-RP
Polar-RP
Fusion-RP

250 x 2.0
4 x 2.0*
/10pk
00G-4337-B0
AJ0-6073
00G-4375-B0
AJ0-7510
00G-4336-B0
AJ0-6075
00G-4424-B0
AJ0-7556
For more dimensions and phases of Axia packed

preparative columns, see pp. 342-343, or contact
your Phenomenex Technical Consultant
Synergi Bulk Media

Beyond our largest preparative column dimensions,
Synergi phases are available in bulk quantities for
HPLC purification at the process, pilot, and commercial
scale. These medias offer a complementary selectivity
to the standard C18, C8, or Silica phases traditionally
employed in larger scale HPLC. Additionally, due to
the diverse chemical properties of each of the Synergi
phases, dramatic differences in chromatographic
parameters such as retention time, selectivity, and
resolution are often observed. For those challenging
purifications where chromatography still makes the
most sense, the Synergi family offers an excellent
alternative to evaluate! Get your Synergi preparative
scout column(s) and evaluate these phases today!
Larger quantities of bulk

media available upon request.
259
Tosoh Bioscience llc

Distributed by Phenomenex in the USA, Canada, and Puerto Rico only.
TSKgel Normal
Phase Columns
Normal Phase Amide-80 Series
Mfr.
Part No.
No. Description
ID (mm)
Stainless Steel Columns
CH0-7989 19694 Amide-80
2.0
CH0-7991 19696 Amide-80
2.0
CH0-7992 19697 Amide-80
2.0
CH0-7993 19532 Amide-80
4.6
CH0-7994 19533 Amide-80
4.6
CH0-7995 13071 Amide-80
4.6
CH0-7996 14459 Amide-80
7.8
CH0-7997 14460 Amide-80
21.5
Guard Columns
CH0-7998 19021 Amide-80 Guard column
4.6
CH0-7999 14461 Amide-80 Guard column
21.5
CH0-8000 42140 Amide-80 Guard cartridge, 2.0
3/pk
CH0-8123 19308 Guard cartridge holder
(For all 2 mm ID columns)
Separate hydrophilic compounds such as peptides, carbohydrates,

and small polar molecules for drug discovery. The TSKgel Amide-80
column offers an excellent alternative to amino bonded stationary
phases and consists of 5 or 10 m silica particles with 80 pores
in a stainless steel format.
Length Particle
(cm) Size (m)
5.0
15.0
25.0
5.0
10.0
25.0
30.0
30.0
5
5
5
5
5
5
10
10
1.0
7.5
1.0
5
10
5
Price
Tosoh Bioscience LLC | HPLC
Size Exclusion ChromatographySW- and PW-Types

Properties and Molecular Weight Separation Ranges for TSKgel
SW-Type Packings
Data generated using the following conditions:
Molecular Weight of Sample (Da)
TSKgel Column
Particle
Size
(m)
Pore
Size
()
Globular
proteins
Dextrans
Polyethylene glycols
and oxides
SuperSW2000
G2000SWXL/BioAssist G2SWXL
QC-PAK TSK 200
G2000SW
4
5
5
10, 13
125
125
125
125
5,0001.5 x 105
5,0001.5 x 105
5,0001.5 x 105
5,0001 x 105
1,0003 x 104
1,0003 x 104
1,0003 x 104
1,0003 x 104
50015,000
50015,000
50015,000
50015,000
SuperSW3000
QC-PAK TSK 300
G3000SW
4
5
5
10
250
250
250
250
1 x 1045 x 105
1 x 1045 x 105
1 x 1045 x 105
1 x 1045 x 105
2,0007 x 104
2,0007 x 104
2,0007 x 104
2,0007 x 104
1,0003.5 x 104
1,0003.5 x 104
1,0003.5 x 104
1,0003.5 x 104
G4000SW
8
13, 17
450
450
2 x 1047 x 106
2 x 1047 x 106
4,0005 x 105
4,0005 x 105
2,0002.5 x 105
2,0002.5 x 105
Columns: Two 4 m, 4.6 mm x 30 cm TSKgel

SuperSW columns in series; two 5 m, 7.8 mm
x 30 cm TSKgel SWxl columns in series; two
10 m, 7.5 mm x 60 cm TSKgel SW columns in
series
Mobile Phases: Globular proteins: 0.3M NaCl
in 0.1 M (0.05 M for SWxl columns) phosphate
buffer, pH 7; Dextrans, polyethylene glycols and
oxides (PEOs): distilled water
PW-Type Packings
Molecular Weight of Sample (Da)
TSKgel Column
G1000PW
G2000PW
G2500PWXL
G2500PW
G3000PWXL
G3000PW
G4000PWXL
G4000PW
G5000PWXL
G5000PW
G6000PWXL/BioAssist G6PWXL
G6000PW
GMPWXL
GMPW
G-Oligo-PW
G-DNA-PW
260
Data generated using the following conditions:
Particle
Size
(m)
Pore
Size
()
Globular
proteins
Dextrans
Polyethylene glycols
and oxides
10
10, 17, 20
6
10, 17, 20
6
10, 17, 20
10
17, 22
10
17, 20, 22
13
17, 25
13
17
6
10
<100
125
<200
up to 1,000
up to 2,000
up to 3,000
<2,000
<5,000
<8,000
200
up to 5 x 104
up to 6 x 104
5008 x 105
500
2,0003 x 104
1,0007 x 105
1 x 1041.5 x 106
1000
4,0001 x 106
5 x 1042.5 x 106
<1 x 107
>1000
4 x 10 8 x 10
5 x 10 5 x 10
<2 x10
<1001000
5008 x 106
<5 x 107
<2 x 108
125
>1000
up to 3,000
4 x 1048 x 106
<5 x 107
<3,000
<2 x 108
Columns: TSKgel PW columns, 7.5 mm x 60

cm; TSKgel PWxl, G-Oligo-PW and G-DNA-PW,
7.8 mm x 30 cm
Mobile Phases: Polyethylene glycols and
oxides: distilled water; Dextrans and proteins:
0.2 M phosphate buffer, pH 6.8
Flow Rates: 1.0 mL/min
Note * Larger particle sizes of each group are for 21.5mm x 60cm
semi-preparative and 55 mm or 108 mm x 60 cm preparative
columns.
** Maximum separation range determined from estimated exclusion
limits.
Phenomenex
Tosoh Bioscience llc

Distributed by Phenomenex in the USA, Canada, and Puerto Rico only.
TSKgel SW- and PW-Type (cont'd)

SW-Type (silica-based)
Part No.
CH0-8001
CH0-7439
CH0-1251
CH0-1252
CH0-1253
CH0-8002
CH0-8003
CH0-0708
CH0-0712
CH0-0716
CH0-8004
CH0-0713
CH0-8005
PEEK Columns
CH0-8126
CH0-8006
Mfr. No.
Description
ID
(mm)
Length
(cm)
Particle
Size
(m)
Min. Number
Theoretical
Plates
Flow Rate (mL/min)

Range
Max.
Maximum
Pressure Drop
(kg/ cm2)
18674
18675
08540
08541
08542
16215
16049
05788
05789
05790
05102
05103
05104
SuperSW2000
SuperSW3000
G2000SWXL
G3000SWXL
G4000SWXL
QC-PAK GFC 200
QC-PAK GFC 300
G2000SW
G3000SW
G4000SW
G2000SW
G3000SW
G4000SW
4.6
4.6
7.8
7.8
7.8
7.8
7.8
7.5
7.5
7.5
7.5
7.5
7.5
30
30
30
30
30
15
15
30
30
30
60
60
60
4
4
5
5
8
5
5
10
10
13
10
10
13
30,000
30,000
20,000
20,000
16,000
10,000
10,000
10,000
10,000
8,000
20,000
20,000
16,000
0.10.35
0.10.35
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.4
0.4
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
120
120
70
70
35
40
40
20
25
15
40
50
30
20027
20026
BioAssist G2SWXL
BioAssist G3SWXL
7.8
7.8
30
30
5
5
20,000
20,000
0.51.0
0.51.0
1.2
1.2
70
70
Length
(cm)
Particle
Size
(m)
Min. Number
Theoretical
Plates
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
30
60
60
60
60
60
60
60
6
10
6
6
10
10
13
13
10
10
10
10
17
17
17
17
10
10
10
17
17
17
17
14,000
10,000
14,000
14,000
10,000
10,000
7,000
7,000
5,000
5,000
5,000
5,000
3,000
3,000
3,000
3,000
10,000
10,000
10,000
6,000
6,000
6,000
6,000
Price
PW-Type (polymer-based)
Phenomenex
Mfr. No.
Description
08031
08032
08020
08021
08022
08023
08024
08025
05760
05761
08028
05762
05763
05764
05765
08026
05105
08029
05106
05107
05108
05109
08027
G-Oligo-PW
G-DNA-PW
G2500PWXL
G3000PWXL
G4000PWXL
G5000PWXL
G6000PWXL
GMPWXL
G1000PW
G2000PW
G2500PW
G3000PW
G4000PW
G5000PW
G6000PW
GMPW
G2000PW
G2500PW
G3000PW
G4000PW
G5000PW
G6000PW
GMPW
7.8
7.8
7.8
7.8
7.8
7.8
7.8
7.8
7.5
7.5
7.5
7.5
7.5
7.5
7.5
7.5
7.5
7.5
7.5
7.5
7.5
7.5
7.5
Flow Rate (mL/min)

Range
Max.
0.50.8
0.20.5
0.50.8
0.50.8
0.30.8
0.30.8
0.30.8
0.30.8
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
0.51.0
1.0
0.6
1.0
1.0
1.0
1.0
1.0
1.0
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
1.2
Maximum
Pressure Drop
(kg/ cm2)
40
20
40
40
20
20
20
20
20
20
20
20
10
10
10
10
40
40
40
20
20
20
20
Price
HPLC | Tosoh Bioscience LLC
Part No.
CH0-8007
CH0-8008
CH0-0748
CH0-0752
CH0-0756
CH0-0760
CH0-0764
CH0-0767
CH0-0739
CH0-0742
CH0-0745
CH0-0749
CH0-0753
CH0-0757
CH0-0761
CH0-8009
CH0-8010
CH0-8011
CH0-0750
CH0-8012
CH0-8013
CH0-8014
CH0-8015
ID
(mm)
261
Ultracarb
High efficiencies
ODS(20) contains 22% carbon load
Excellent peak shape for basic compounds
ODS(30) contains 31% carbon load
The Ultracarb ODS(20) contains a 22 % carbon load and gives

excellent separations of free fatty acids, triglycerides, fat-soluble
vitamins, and other lipophilic compounds.
The Ultracarb ODS(30) contains one of the highest commercially

available carbon loadings, at 31 %. For separating basic compounds, making sample quantification simple and reliable.
Ultracarb C8 was introduced to offer a high carbon load material

with somewhat different selectivity than the two Ultracarb ODS
phases.
Minibore and MidBore Columns (mm)

Phases
150 x 2.0
3 m ODS (20)
5 m ODS (20)
5 m ODS (30)
00F-0351-B0
150 x 3.2
250 x 3.2
00F-0206-R0
00F-0351-R0
00G-0206-R0

4 x 2.0
4 x 3.0
/10pk
/10pk
AJ0-4286
AJ0-4287
AJO-4286
AJ0-4287
AJO-4286
AJ0-4287
for ID: 2.0-3.0 mm
3.2-8.0 mm
Analytical Columns (mm)

Phases
50 x 4.6
Ultracarb / ultrEmex / vydac | HPLC
3 m ODS (20)
5 m C8
5 m ODS (20)
5 m ODS (30)
7 m ODS (30)
00B-0205-E0
00B-0351-E0
100 x 4.6
150 x 4.6
250 x 4.6
00D-0205-E0
00D-0206-E0
00D-0351-E0
00F-0205-E0
00F-2134-E0
00F-0206-E0
00F-0351-E0
00G-0205-E0
00G-2134-E0
00G-0206-E0
00G-0351-E0*
00G-0352-E0
SecurityGuard
Cartridges (mm)
4 x 3.0
/10pk
AJ0-4287
AJ0-4290
AJ0-4287
AJ0-4287
AJ0-4287
for ID: 3.2-8.0 mm
*IMPORTANT: Phenomenex highly recommends

the use of 150 mm column length, as opposed
to the traditional 250 mm column length, when
the 5 m ODS (30) phase is desired. In those
cases when the additional retention and resolution of a
250 mm column is desired, please be aware that column
backpressure with Ultracarb 5 m ODS (30) can be 50 to
100 % higher than that experienced with standard ODS
columns. This relatively high backpressure is a function
of the hydrophobicity of the 5 m ODS(30) phase; higher
backpressure is completely natural with this phase
and will have no ill consequence for the column.
Ultremex
If you do not find Ultremex to be a

direct alternative to Ultrasphere with
the same phase, particle size, and
FULL REFUND.
Ultremex is a GUARANTEED alternative

to Ultrasphere at a significant cost savings
Ultremex is designed to closely mimic
the performance of Ultrasphere
Long column life

Substantial savings per column
Highly reproducible
Ordering Information and Cross-Reference Chart
Ultrasphere
Description (mm)
3m
C8
75 x 4.6
C18
75 x 4.6
5m
C8
150 x 4.6
C8
250 x 4.6
C18
150 x 2.0
C18
150 x 4.6
C18
250 x 4.6
C18 IP 250 x 4.6
Part No.
Phenomenex Ultremex
Part No.
Description (mm)
244228
244254
00C-0038-E0
00C-0039-E0
75 x 4.6
75 x 4.6
235333
235332
237390
235330
235329
235335
00F-0047-E0
00G-0047-E0
00F-0048-B0
00F-0048-E0
00G-0048-E0
00G-0048-E0
150 x 4.6
250 x 4.6
150 x 2.0
150 x 4.6
250 x 4.6
250 x 4.6
VYDAC
Price
Are you still using Ultrasphere Guards?

Upgrade to SecurityGuard
Ultrasphere Guards
Phase
Ultrasphere Octyl (C8)
Ultrasphere ODS (C18)
Ultrasphere ODS Ion Pair (IP)
Ultrasphere Si (Silica)
Ultrasphere Cyano (CN)
Part No.
243532
243533
235329
244011
244072
Phenomenex SecurityGuard Replacement*

Part No.
Price: 10/pk
AJ0-4290
AJ0-4287
AJ0-4287
AJ0-4348
AJ0-4305
Guard Holder
Part No.
Holder Assembly, XL Columns 237060
Guard Holder
KJ0-4282
Price: kit
*Protects 3.2-8.0 mm ID columns
By Alltech Associates, Inc.

262
Phenomenex
Whatman *
Standard and Void-Sealing HPLC Columns

Spherical and irregular silicas
Void sealing hardware features:
Reusable end fittings, disposible column
Built-in void compensator
Packing Material
Partisil Silica
Partisil C8
Partisil ODS
Partisil ODS-2
Partisil ODS-3
Partisil PAC
Partisil SAX
Partisil SCX
PartiSphere Silica
PartiSphere C8
PartiSphere C18
PartiSphere SAX
PartiSphere SCX
PartiSphere WAX
PartiSphere WCX
Particle
Shape/Size
(m)
Pore
Size
()
Surface
Area
(m2/g)
Carbon
Load
%
Calculated Bonded
Phase Coverage
(mole/m2)
End
Capping
Irreg. 5, 10
Irreg. 5, 10
Irreg. 10
Irreg. 5
Irreg. 5, 10
Irreg. 10
Irreg. 10
Irreg. 5, 10
Spher. 5
Spher. 5
Spher. 5
Spher. 5
Spher. 5
Spher. 5
Spher. 5
85
85
85
85
85
85
85
85
120
120
120
120
120
120
120
350
350
350
350
350
350
350
350
160
160
160
160
160
160
160
0
8.5
5
16
10.5
0.85(N)
0.85(N+R3)
0.40(S)
0
6
10
0.85(N+R3)
0.40(S)
0.50(N)
0.7
0
2.33
0.63
2.43
1.45
0
3.44
3.01
No
Yes
No
No
Yes
No
No
No
No
Yes
Yes
No
No
No
No
For Phenomenex packed Partisil

HPLC columns, see p. 215
Column Type
Analytical
RAC II
Analytical
RAC II
Analytical
RAC II
Analytical
Analytical
Analytical
Analytical
Analytical
Size (mm)
250 x 4.6
100 x 4.6
250 x 4.6
100 x 4.6
250 x 4.6
100 x 4.6
250 x 4.6
250 x 4.6
250 x 4.6
250 x 4.6
250 x 4.6
Price
Standard Semi-Preparative and Preparative Columns

Part No.
Mfr. No.
Description
CH0-2217
4230-225
Partisil 10 m ODS-3
Column Type
Magnum 9
Size (mm)
500 x 9.4
Price
WVS1 Columns*
Part No.
CH0-2223
CH0-2240
CH0-2246
CH0-2248
CH0-2252
CH0-2253
CH0-2256
CH0-2257
Mfr. No.
4681-1502
4682-1505
4621-0502
4621-0503
4621-0505
4621-1505
4621-0507
4621-1507
Description
Partisil 5 m ODS-3
Partisil 10 m SAX
PartiSphere 5 m C18
PartiSphere 5 m C8
PartiSphere 5 m SAX
PartiSphere 5 m SAX
PartiSphere 5 m SCX
PartiSphere 5 m SCX
Size (mm)
250 x 4.6
250 x 4.6
125 x 4.6
125 x 4.6
125 x 4.6
250 x 4.6
125 x 4.6
250 x 4.6
Price
HPLC | Whatman
Whatman Standard HPLC (WCS2) Columns
Part No.
Mfr. No.
Description
CH0-2136
4215-001
Partisil 5 m Silica
CH0-2142
4222-225
Partisil 5 m ODS-3
CH0-2143
4238-001
Partisil 5 m ODS-3
CH0-2145
4222-232
Partisil 5 m C8
CH0-2146
4239-001
Partisil 5 m C8
CH0-2154
4222-228
Partisil 5 m SCX
CH0-2159
4223-001
Partisil 10 m ODS
CH0-2161
4228-001
Partisil 10 m ODS-3
CH0-2165
4225-001
Partisil 10 m PAC
CH0-2168
4226-001
Partisil 10 m SAX
CH0-2170
4227-001
Partisil 10 m SCX
All other Whatman columns

and accessories not listed are
available upon request.
WVS1 Hardware
Part No.
CH0-2299
Mfr. No.
4631-1001
Description
WVS Column End-fittings 1 pair, 4.6 mm
Price
1
WVS = Whatman Void Sealing.
2
WCS = Whatman Compression Screw.
*Replacement column only, end-fittings sold separately.
*Partisil and PartiSphere are registered trademarks of Whatman Inc.
Phenomenex
263
If any Phenomenex-packed Zorbax column does not

provide equal or better results than other manufactured
columns using Zorbax material of 7 m particle size with
the same phase and dimensions, return the column with
comparative data within 45 days for a full refund.
Zorbax
Packed by Phenomenex
High efficiency, guaranteed quality
Packing
Material
Zorbax ODS
Zorbax C8
Particle
Shape/Size
(m)
Pore
Size
()
Spher. 3, 5, 7
Spher. 3, 5, 7
70
70
Surface Carbon
Area Load
(m2/g) %
300
300
Calculated
Bonded Phase
Coverage
(mole/m2)
End
Capping
3.47
2.99
Yes
Yes
20, Monomeric
12, Monomeric

Phases
150 x 4.6
C8
ODS
250 x 4.6
00F-0087-E0
00F-0088-E0
00G-0087-E0
00G-0088-E0

4 x 3.0
/10pk
AJ0-4290
AJ0-4287
for ID: 3.2-8.0 mm
Zorbax
zorbax | HPLC
Manufactured by Agilent Technologies

Eclipse XDB Columns
Column
Analytical
Analytical
Rapid Resolution
Rapid Resolution
Solvent Saver Plus
Narrow-Bore
Narrow-Bore LC/MS
Guard Cartridge1 (4pk)
m
5
5
3.5
3.5
3.5
5
5
5
StableBond 80 Columns
Column
Analytical
Analytical
Rapid Resolution
Rapid Resolution
Solvent Saver
Solvent Saver Plus
Narrow-Bore
Narrow-Bore LC/MS
Semi-Preparative
Preparative
m
5
5
3.5
3.5
5
3.5
5
5
5
7
5
Size (mm)
250 x 4.6
150 x 4.6
150 x 4.6
75 x 4.6
150 x 3.0
150 x 2.1
50 x 2.1
12.5 x 4.6
Size (mm)
250 x 4.6
150 x 4.6
150 x 4.6
75 x 4.6
250 x 3.0
150 x 3.0
150 x 2.1
50 x 2.1
250 x 9.4
250 x 21.2
12.5 x 4.6
XDB-C18 (L1)
990967-902
993967-902
963967-902
966967-902
963954-302
993700-902
960967-902
820950-925
XDB-C8 (L7)
990967-906
993967-906
963967-906
960967-906
820950-926
SB-C18 (L1)
880975-902
883975-902
863953-902
866953-902
883700-922
860975-902
Size (mm)
250 x 4.6
150 x 4.6
150 x 4.6
75 x 4.6
250 x 3.0
150 x 2.1
250 x 21.2
12.5 x 4.6
Rx-C18 (L1)
880967-902
883967-902
863967-902
866967-902
820950-914
Traditional Reversed-Phase Columns

Column
m
Analytical
5
Analytical
5
5
Size (mm)
250 x 4.6
150 x 4.6
12.5 x 4.6
ODS C18 (L1)

880952-702
883952-702
820950-902
Reusable Hardware Kits

Kit
Guard Hardware Kit*
m
5
5
5
5
Size (mm)
250 x 4.6
150 x 4.6
250 x 9.4
12.5 x 4.6
Part No.
820999-901
Rx-SIL (L3)
880975-901
883975-901
Rx-C8 (L7)
880967-901
883967-901
863953-906
866953-906
880975-306
883700-906
877250-106
820950-913
C8 (L7)
880952-706
883952-706
820950-906
SIL (L3)
880952-701
883952-701
SB-CN (L10)
880975-905
883975-905
863953-905
866953-905
883700-905
860975-905
880975-205
820950-916
SB-Phenyl (L11)
880975-912
883975-912
863953-912
866953-912
863954-312
Price
Guard cartridges require a reusable

hardware kit, Part No. 820999-901
(see ordering information below).
2
Non-endcapped
Price
All hardware components are available

separately as replacement parts.
Phenyl (L11)
880952-712
CN (L10)
880952-705
883952-705
820950-905
CN (L10)
884950-507
884950-526
820950-905
NH2 (L8)
880952-708
883952-708
820950-908
TMS (L13)
880952-710
Price
Price
Price
* Guard Hardware Kit includes guard column holder (1), guard endfitting (1), column connector (1).
Upgrade for old Part No. 820888-901. Compatible with all Zorbax guard cartridges.
264
SB-C8 (L7)
880975-906
883975-906
863953-906
866953-906
880975-306
863954-306
877250-106
Rx 80 Reversed-Phase HPLC Columns

Column
m
Analytical
5
Analytical
5
Rapid Resolution
3.5
Rapid Resolution
3.5
Solvent Saver
5
Narrow-Bore
5
Preparative
7
Guard Cartridge1(4pk)
5
Normal Phase Columns

Column
Analytical
Analytical
Semi-Preparative
StableBond 300 (Wide Pore)

columns available
Price
ZORBAX is a registered trademark of Agilent Technologies, Inc.

Phenomenex is not affiliated with Agilent Technologies Inc.
Phenomenex
Harness the Power of Core-Shell Technology

on your UHPLC Systems
UHPLC
UHPLC
UHPLC Columns

Core-Shell Technology: The Key to Better UHPLC Performance.......... 266
Kinetex 1.7 m and 2.6 m Core-Shell Technology Columns............... 267
Aeris Core-Shell Technology for RP-LC of Proteins & Peptides............ 272
Clarity Core-Shell Columns for Synthetic DNA/RNA............................. 275
SecurityGuard ULTRA Column Protection............................................. 276
265 - 276
UHPLC
The Key to Better UHPLC
Performance is Core-Shell
Technology
Core-shell particle technology was precision engineered to provide
increased efficiencies and improved performance compared to traditional fully porous sub-2 m particles on UHPLC systems.
The precise architecture of core-shell particles provides dramatic leaps in

performance in two important ways:
The thin, porous layer, or shell, decreases the diffusion

path length, thus reducing the time it takes for molecules to
diffuse into and out of the particle.
Core-Shell Particle
Uniform sizing and shape of the particles along with tight

packing specifications reduces losses in efficiency and performance due to band broadening.
Core-Shell Particles
Band Broadening
Band Broadening
Fully Porous Particles
core-shell technology
UHPLC
Fully Porous Particle
Band Broadening
Band Broadening
The result is...

Kinetex Core-Shell Columns for Small Molecules, page 267
2.6 m core-shell particles that can perform like sub-2 m columns at lower backpressures, allowing for faster flow rates and
higher throughput
1.7 m core-shell particles that can provide higher efficiencies compared to fully porous sub-2 m columns on UHPLC systems
Aeris Core-Shell Columns for Proteins and Peptides, page 272

3.6 m core-shell particles that can perform like sub-2 m columns on UHPLC systems at a fraction of the backpressure,
allowing for longer (and coupled) columns to be used for improved resolution
1.7 m core-shell particles that can provide higher peak capacities and improved resolution compared to fully porous sub-2 m
columns on UHPLC systems
Clarity Oligo-MS Core-Shell Columns for Synthetic DNA/RNA, page 275

2.6 m core-shell particles that can deliver increased efficiencies at reduced backpressures for method flexibility
1.7 m core-shell particles that can provide higher efficiencies compared to fully porous sub-2 m columns on UHPLC systems
266
Phenomenex
Kinetex

a FULL REFUND.
Use Kinetex 1.7 m Columns

for up to 20% Higher
Efficiencies than Fully Porous
Sub-2 m Columns*
1.7 m Core-Shell Particle

1.25 m Solid Core
0.23 m Porous Shell
Kinetex core-shell 1.7 m and 2.6 m core-shell particles were

engineered to provide increased efficiencies and improved performance compared to traditional fully porous sub-2 m particles on
UHPLC systems.
1.7 m
Increase resolution, throughput, and sensitivity

Save time and money
2.6 m Core-Shell Particle
1.7 m and 2.6 m particles are directly scalable
1.9 m Solid Core
Available in C18, XB-C18, C8, PFP, HILIC, and Phenyl-Hexyl

phases
0.35 m Porous Shell
1.7 m Kinetex core-shell columns are the FIRST and ONLY

sub-2 m core-shell particles on the market, and produce up to
20 % higher efficiencies than fully porous particles, taking UHPLC
to the next level.
2.6 m
Efficiency Test Mix

18167
Kinetex 1.7m C18

N = 318,680 p/m
mV
17 % Higher Efficiency
than the traditional fully
porous sub-2m column
400
Waters ACQUITY BEH 1.7m C18
18165
N = 272,080 p/m
mV
400
1
200
min

Columns: Kinetex 1.7m C18
ACQUITY UPLC BEH 1.7m C18
Injection Volume: 0.2 L/min
4
3
0
0
kinetex
App ID 18165
3
App ID 18167
UHPLC |
200
min
Temperature: 25 C
Detection: UV @ 254nm (ambient)
Sample: 1. Acetophenone
2. Benzene
3. Toluene
4. Naphthalene
*Columns made with Kinetex 1.7 m core-shell particles demonstrate exceptional hydrodynamic and mass-transfer properties. Depending on the application and conditions, their performance has been shown to surpass that of columns made with fully porous sub-2 m
particles by more than 20 % [1]. Furthermore, the advantage in performance of core-shell particles over fully porous sub-2 m particles
increases significantly at high flow rates [2].
1. Fekete et al., J. Pharm. Biomed. Analysis 54 (2011) 482
2. Gritti et al., J. Chromatogr. A (2010) 5069
Waters, ACQUITY, and UPLC are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Phenomenex is in no way
affiliated with Waters Corporation. Comparative separations may not be representative of all applications.
Phenomenex
267
Kinetex
Kinetex 2.6 m Columns

Provide Sub-2m Efficiencies
at Significantly Lower
Backpressures
Ultra-High
Backpressure
Not Required
Using a 2.6 m Kinetex core-shell column on your UHPLC system

provides sub-2 m efficiencies with significantly lower backpressure, so you can run higher flow rates, use longer length columns
for increased resolving power, and easily transfer your methods to
HPLC systems.
18165
mV
Traditional FullyPorous Sub-2 m C18 Column

1
400
N = 272,080 p/m
> 400 bar!
Method cannot transfer to
a non-UHPLC instrument
18166
mV
Kinetex Core-Shell 2.6 m C18 Column

N = 267,720 p/m
400
< 300 bar!

Easier method transfer
to ANY system
4
3
App ID 18165
kinetex | uHPLC
200
App ID 18166
200
0
0

Temperature: 25 C
2 min
2 min
Sample: 0.5 L test mixture
1. Acetophenone 3. Toluene
2. Benzene
4. Naphthalene
Core-shell technology is at the forefront of UHPLC technology and is challenging system manufacturers to develop the next generation
UHPLC systems capable of taking full advantage of the performance offered.1
1. F. Gritti, C.A. Sanchez, T. Farkas, G. Guiochon, J.Chromatogr. A 1217 (2010) 3000-3012
Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation. Phenomenex is in no way affiliated with Waters Corporation.
268
Phenomenex
Kinetex
Improved UHPLC Performance over

Fully Porous Sub-2 m Particles
Delivering breakthrough UHPLC performance, Kinetex 1.7 m and
2.6 m core-shell columns produce increased efficiencies over
traditional sub-2 m columns on the market, yielding remarkable
Kinetex 1.7m XB-C18
19799
AU
0.014
0.008
0.012
19% Higher Peak Capacity

than the traditional fully
porous sub-2m column
0.01
Peak Capacity = 231
AU
0.014
0.012
19800
Peak Capacity = 276
chromatographic resolution, higher peak capacities, and greater

sensitivity, so you can get the most out of every analysis.
0.01
3
0.008
0.004
0.004
App ID 19799
0.006
0.002
0
-0.002
App ID 19800
3
0.006
0.002
0
-0.002
-0.004
-0.004
0.8
1.2
1.4
1.6
1.8
2.2
2.4 min
0.8

Column: Kinetex 1.7m XB-C18
ACQUITY UPLC BEH 1.7m C18
Mobile Phase: A: 6.9mM Ammonium Formate pH 4.0 in 25% Acetonitrile
B: Acetonitrile
Gradient: Time (min) % B
0
0
5
65
Flow Rate:
Temperature:
Detection:
Instrument:
Sample:
1.2
1.4
1.6
1.8
2.2
min
2.4
0.5 mL/min
Ambient
UV @ 254 nm (ambient)
Waters ACQUITY UPLC
1. Estriol
2. 17B Estradiol
3. Estrone
Higher peak capacity than ACQUITY and higher flow rates make it even better

19861
Peak Capacity: 43.5 @ 7700 psi (531 bar)
9.0e5
9 10
11
1.1e6
3.0e5
1
1.0e5
5.0e4
0.0
4
6
15
0.2
8
0.4
0.6
12
0.8
13
1.0
3
1.2
Intensity, cps
5.0e5
2.0e5
9.0e5
14
4.0e5
11
1.0e6
App ID 19862
Intensity, cps
1.2e6
26% Increase in
Peak Capacity
8.0e5
6.0e5
1.3e6
1.0e6
7.0e5
Peak Capacity: 34.4 @ 7700 psi (531 bar)
1.4e6
8.0e5
7.0e5
6.0e5
1.4
1.6
1.8
2.0
2.2
min
5.0e5
4.0e5
4
3.0e5
2.0e5
1.0e5

Mobile Phase: A: 0.1% Formic acid in Acetonitrile
B: 0.1% Formic acid (aq)
%B
0.00
95
0.25
95
1.80
10
1.90
10
1.91
95
2.50
95
Temperature: 40 C
Detection: MS
10
14
App ID 19861
1.1e6
0.0
uHPLC | kinetex
Kinetex 2.6 m C18
19862
6
5
15
0.2
0.4
0.6
Instrument: Waters ACQUITY Binary UPLC

API 5000 QQQ
Flow Rate: Kinetex: 1.4 mL/min
Traditional: 0.8 mL/min
Sample: 1. Haloperidol
2. Diltiazem
3. Terfenadine
4. Cimetidine
5. Acetaminophen
6. Sulfathiazole
7. Pindolol
8. Quinidine
12
0.8
1.0
13
1.2
1.4
1.6
1.8
2.0
2.2
min
9. Acebutolol
10. Chlorpheniramine
11. Triprolidine
12. Prednisolone
13. Nortriptyline
14. 2-hydroxy-5-methyl benzaldehyde
15. Hexanophenone
Data generated by Quotient Bioresearch, U.K.
It Doesnt Stop Here!

ACQUITY and UPLC are registered trademarks, and BEH Technology is a trademark of Waters Corporation.
API 5000 is a trademark of AB SCIEX. Phenomenex is in no way affiliated with Waters Corporation.
Phenomenex
34 % increase in peak capacity and a 3.5 xs increase in sensitivity

was achieved!
Request Technical Note TN-1104 and learn how.
269
Kinetex
Six Selectivities. Take Your Pick

Choose from a wide range of phases for greater flexibility in UHPLC
method development. Kinetex columns come in a variety of selectivities to cover a full spectrum of applications ranging from acids and
bases, to isomers and extremely polar compounds.
ACN H20 ACN
ACN
ACN
ACN H 0
2
TMS
TMS
TMS
Si
TMS
Increased retention
for polar basic
compounds
TMS
Protective isobutyl
side chains
Increased retention
of polar acidic
compounds
PFP
C8
Endcapped C8 phase
H20
H20
ACN H 0 ACN
2
OH
H20
H20
Si
ACN
ACN
H20
H20
HO
H0
HO
ACN
HO
H20
ACN
ACN
ACN
Si
HO
Si
Endcapped C18 phase
TMS
TMS
XBC18
C18
TMS
ACN
ACN
ACN
TMS
TMS
H20
OH
PhenylHexyl
HILIC
Pentafluorophenyl phase Unbonded silica phase Endcapped Phenyl Phase

Unique aromatic
and polar selectivity
Less hydrophobic
than a C18 phase
Increased retention
of polar compounds
Increased retention of
aromatic hydrocarbons
kinetex | uHPLC
Packing
Material
Kinetex XB-C18
Kinetex C18
Kinetex C8
Kinetex PFP
Kinetex HILIC
Kinetex XB-C18
Kinetex C18
Kinetex C8
Kinetex PFP
Kinetex HILIC
Total Particle Size

(m)
Porous Shell
(m)
Solid Core
(m)
Pore Size
(A)
Effective Surface Area

(m2/g)
Effective Carbon Load

%
pH
Stability
1.7
1.7
1.7
1.7
1.7
1.7
2.6
2.6
2.6
2.6
2.6
2.6
0.23
0.23
0.23
0.23
0.23
0.23
0.35
0.35
0.35
0.35
0.35
0.35
1.25
1.25
1.25
1.25
1.25
1.25
1.9
1.9
1.9
1.9
1.9
1.9
100
100
100
100
100
100
100
100
100
100
100
100
200
200
200
200
200
200
200
200
200
200
200
200
10
12
8
9
0
11
10
12
8
9
0
11
1.5 - 8.5**
1.5 - 8.5**
1.5 - 8.5**
1.5 - 8.5**
2.0 - 7.5
1.5 - 8.5**
1.5 - 8.5**
1.5 - 8.5**
1.5 - 8.5**
1.5 - 8.5**
2.0 - 7.5
1.5 - 8.5**
Pressure
Stability
1000
bar
1000/600
bar
**Columns are pH stable from 1.5-10 under isocratic conditions. Columns are pH stable 1.5-8.5 under gradient conditions.
2.1 mm ID Kinetex columns are pressure stable up to 1000 bar.
When using Kinetex 1.7 m, increased performance can be achieved, however higher pressure-capable instrumentation is required.
270
Phenomenex
Kinetex
2010 R&D 100

Award Recipient
Phases
30 x 2.1
XB-C18
C18
C8
PFP
HILIC
Phenyl-Hexyl
00A-4498-AN
00A-4475-AN
00A-4499-AN
00A-4476-AN
00A-4474-AN

Phases
30 x 3.0
XB-C18
C18
C8
PFP
HILIC
Phenyl-Hexyl
00A-4498-Y0
00A-4499-Y0

Phases
30 x 2.1
XB-C18
C18
C8
PFP
HILIC
Phenyl-Hexyl
00A-4496-AN
00A-4462-AN
00A-4497-AN
00A-4477-AN
00A-4461-AN

Phases
30 x 3.0
00A-4496-Y0
00A-4462-Y0
00A-4497-Y0
00A-4477-Y0
00A-4461-Y0
XB-C18
C18
C8
PFP
HILIC
Phenyl-Hexyl
00A-4462-E0
00A-4477-E0
00A-4461-E0
150 x 2.1
00B-4498-AN
00B-4475-AN
00B-4499-AN
00B-4476-AN
00B-4474-AN
00B-4500-AN
00D-4498-AN
00D-4475-AN
00D-4499-AN
00D-4476-AN
00D-4474-AN
00D-4500-AN
00F-4498-AN
00F-4475-AN
00F-4499-AN
00F-4476-AN
00F-4500-AN
50 x 3.0
100 x 3.0
SecurityGuard
Ultra Cartridges
3/pk
00B-4498-Y0
00B-4475-Y0
00B-4499-Y0
00B-4474-Y0
00D-4498-Y0
00D-4475-Y0
00D-4499-Y0
00D-4476-Y0
AJ0-8782
AJ0-8782
AJ0-8784
AJ0-8787
AJ0-8786
AJ0-8788
for 2.1 mm ID
AJ0-8775
AJ0-8775
AJ0-8777
AJ0-8780
AJ0-8779
AJ0-8781
for 3.0 mm ID
50 x 2.1
100 x 2.1
150 x 2.1
00B-4496-AN
00B-4462-AN
00B-4497-AN
00B-4477-AN
00B-4461-AN
00B-4495-AN
00D-4496-AN
00D-4462-AN
00D-4497-AN
00D-4477-AN
00D-4461-AN
00D-4495-AN
00F-4496-AN
00F-4462-AN
00F-4497-AN
00F-4477-AN
00F-4461-AN
SecurityGuard
Ultra Cartridges
3/pk
AJ0-8782
AJ0-8782
AJ0-8784
AJ0-8787
AJ0-8786
AJ0-8788
for 2.1 mm ID
50 x 3.0
75 x 3.0
100 x 3.0
150 x 3.0
00B-4496-Y0
00B-4462-Y0
00B-4497-Y0
00B-4477-Y0
00C-4496-Y0
00C-4462-Y0
00C-4497-Y0
00C-4477-Y0
00D-4496-Y0
00D-4462-Y0
00D-4497-Y0
00D-4477-Y0
00F-4496-Y0
00F-4462-Y0
00F-4497-Y0
00F-4477-Y0
00F-4461-Y0
50 x 4.6
75 x 4.6
100 x 4.6
150 x 4.6
00B-4496-E0
00B-4462-E0
00B-4497-E0
00B-4477-E0
00B-4461-E0
00B-4495-E0
00C-4496-E0
00C-4462-E0
00C-4497-E0
00C-4477-E0
00C-4461-E0
00D-4496-E0
00D-4462-E0
00D-4497-E0
00D-4477-E0
00D-4461-E0
00D-4495-E0
00F-4496-E0
00F-4462-E0
00F-4497-E0
00F-4477-E0
00F-4461-E0
00F-4495-E0
SecurityGuard
Ultra Cartridges
3/pk
AJ0-8775
AJ0-8775
AJ0-8777
AJ0-8780
AJ0-8779
AJ0-8781
for 3.0 mm ID
SecurityGuard
Ultra Cartridges
3/pk
kinetex
2.6 m Analytical Columns (mm)

Phases
30 x 4.6
100 x 2.1
HPLC |
XB-C18
C18
C8
PFP
HILIC
Phenyl-Hexyl
50 x 2.1
SecurityGuard
Ultra Cartridges
3/pk

a FULL REFUND.
AJ0-8768
AJ0-8768
AJ0-8770
AJ0-8773
AJ0-8772
AJ0-8774
for 4.6 mm ID
SecurityGuard ULTRA cartridges require holder, Part No. AJ0-9000, see p. 239.
Phenomenex
271
Aeris
If Aeris core-shell columns do not provide

same phase, return the column with the
FULL REFUND.
Precision Engineered CoreShell Particles for Ultra-High

Resolution BioSeparations
Aeris is a specialized line of reversed phase core-shell UHPLC columns, built exclusively for the ultra-high performance separation
and analysis of proteins and peptides.
For more in-depth information about Aeris core-shell columns and

to select the right column for your application, please visit p. 284
Core-shell particle technology provides striking increases in peak

capacity and resolution at lower backpressures, giving chromatographers the ability to use longer (or coupled) columns for BioSeparations. Additionally, optimizing the pore size and shell thickness
for intact proteins or smaller peptide fragments leads to maximum
separation power.
Aeris PEPTIDE
Aeris PEPTIDE Columns for Peptide and

Peptide Mapping Separations
Aeris PEPTIDE particles are designed with small pores, inert XBC18 surface chemistry, and two different particle sizes (3.6m and
1.7m) to meet the resolution demands of chromatographers performing complex peptide and peptide map separations on HPLC
and/or UHPLC systems.
3.6 m
Recommended for:
Synthetic peptide impurity analysis
1.7 m
Peptide mapping
Identifying protein modifications
Glycosylation, Substitution, and Truncation
Aeris | uHPLC
Analyzing post-translational modifications

Deamidation, Oxidation, and Deletions
3.6---3.2 solid core
Aeris WIDEPORE Columns for Intact

Protein and Polypeptide Separations
Aeris WIDEPORE columns are packed with 3.6m core-shell
particles that are specially engineered with a thin porous shell,
large pores, and sterically protected XB surface chemistry resulting in low backpressures, fast rates of diffusion, and excellent
selectivity, generating exceptional chromatographic resolution on
UHPLC systems.
Small pore optimized for peptides

and for peptide mapping
Aeris WIDEPORE
3.6 m
Recommended for:
Protein structural characterization
Stability indicating assays

Post-translational modification identification
PEGylated proteins, antibodies, biogenerics, etc.
Impurity profiling
Large pore optimized for intact

proteins and polypeptides
Peptide mapping
272
Phenomenex
Aeris
Maximize UHPLC Resolving Power with Unique 3.6m Core-Shell Particle

3.6m core-shell technology combined with inert surface chemistries and tight packing specifications results in Aeris WIDEPORE
columns delivering exceptional resolving power at significantly
lower backpressures. Chromatographers now have the ability to
generate higher quality data than typically produced by columns

packed with fully porous particles for every protein analysis.
Performance Equivalent to Sub-2m Particle at Low Backpressure
Waters ACQUITY BEH 300 1.7m C18
19912
Aeris WIDEPORE 3.6m XB-C18
AppID 19838
mAU
mAU
1
80
UHPLC Results at
/3 the Backpressure
80
420bar
60
20
0
16
18
20
22
24
26

Column: ACQUITY BEH 300 1.7m C18
Mobile Phase: A: Water with 0.1% TFA
B: Acetonitrile with 0.1% TFA
28
20
0
16
min
Gradient:
Flow Rate:
Temperature:
Injection Volume:
Instrument:
140bar
40
App ID 19912
40
App ID 19838
60
18
20
22
24
26
28
min

Sample: Human Epidermal Growth Factor (EGF)
A/B (97:3) to A/B (35:65) over 45min

0.3mL/min
40C
10L
Agilent 1200
Increase Efficiency on UHPLC Systems with Sub-2m Core-Shell Particles

size results in extremely high efficiencies that scientists can use to
pull apart critical peaks.
Ultra-High Resolution Achieved with 1.7m Core-Shell Technology
Aeris PEPTIDE 3.6m XB-C18
19897
3.6m
19894
uHPLC | aeris
Aeris PEPTIDE 1.7m core-shell columns are an excellent solution

for ultra-high resolution peptide and peptide mapping separations.
Core-shell particle technology combined with a sub-2m particle
1.7m
AU
0.30
Increased Resolution
and Sensitivity
AU
0.20
App ID 19897
App ID 19894
0.10
0.10
0
0

Column: Aeris PEPTIDE 3.6m XB-C18
Part Nos.: 00F-4057-AN
00F-4056-AN
3.6---3.210
solid core
12
min
3.6---2.62solid core

Gradient: A/B (97:3) for 1.5min to A/B (60:40) over 11min to
A/B (5/95) for 1min
Temperature:
Injection Volume:
Instrument:
Detection:
Sample:
8 solid core 10
1.7---1.25
12
min
40C
5L
Agilent 1200SL
UV @ 214nm (ambient)
Alpha-Casein Tryptic Digest
Waters and ACQUITY are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Phenomenex is not affiliated with Waters Corporation. Study was performed using new columns and, to the
extent possible, identical experimental conditions were applied. Comparative separations may not be representative of all applications.
Phenomenex
273
Aeris

FULL REFUND.
Greater Method Flexibility with Specialty

Surface Chemistries
Aeris WIDEPORE columns are available in three surface chemistries
(XB-C18, XB-C8, C4) to satisfy applications of all types, ranging from
sticky, intact proteins to complex protein digests.
Aeris PEPTIDE columns utilize the XB-C18 chemistry, as it is
excellent for peptides and peptide mapping applications.
The unique, sterically protected XB surface ligands are designed by

bonding bulky isobutyl chains aside the alkyl chains, and then fully
endcapping the surface to cover any remaining exposed silanols.
Packing Material
Total Particle Size

(m)
Porous Shell
(m)
Core Size
(m)
Aeris WIDEPORE
Aeris PEPTIDE
Aeris PEPTIDE
3.6
1.7
3.6
0.2
0.22
0.5
3.2
1.25
2.6
pH
Temp
Stability Stability
Pressure
Stability
1.5 - 9
1.5 - 9
1.5 - 9
600bar
1000bar
600bar
90C
90C
90C
Protective isobutyl
side chains
TMS
Si
TMS
TMS
Aeris | uHPLC
XBC18
TMS
TMS
XBC8
TMS
C4
Aeris PEPTIDE 1.7 m Minibore Columns (mm)
Phases
50 x 2.1
100 x 2.1
XB-C18
00B-4506-AN
00D-4506-AN

Phases
50 x 2.1
100 x 2.1
XB-C18
00B-4507-AN
00D-4507-AN
XB-C18
00D-4507-E0
150 x 2.1
00F-4506-AN
150 x 2.1
250 x 2.1
00F-4507-AN
00G-4507-AN
Aeris PEPTIDE 3.6 m Analytical Columns (mm)

Phases
100 x 4.6
150 x 4.6
274
Si
00F-4507-E0
250 x 4.6
Aeris WIDEPORE 3.6 m Minibore Columns (mm)

Phases
50 x 2.1
100 x 2.1
150 x 2.1
250 x 2.1
XB-C18
00B-4482-AN
00D-4482-AN
00F-4482-AN
00G-4482-AN
XB-C8
00B-4481-AN
00D-4481-AN
00F-4481-AN
00G-4481-AN
C4
00B-4486-AN
00D-4486-AN
00F-4486-AN
00G-4486-AN
Aeris WIDEPORE 3.6 m Analytical Columns (mm)

Phases
100 x 4.6
150 x 4.6
250 x 4.6
XB-C18
00D-4482-E0
00F-4482-E0
00G-4482-E0
XB-C8
00D-4481-E0
00F-4481-E0
00G-4481-E0
C4
00D-4486-E0
00F-4486-E0
00G-4486-E0
00G-4507-E0
Phenomenex
Clarity
Core-Shell Columns for Synthetic DNA/RNA

Evaluate Clarity BioSolutions in
your lab for 45 days, if you are not
completely satisfied return it for a
full refund.
Clarity Oligo-MS UHPLC Columns

Rapid and Efficient LC/MS Separation for
QC and Characterization
Core-shell particle technology provides improved speed,
resolution, and sensitivity
Clarity Oligo-MS, C18 columns have been engineered for the MS
characterization of synthetic DNA and RNA samples. This media
is based on core-shell technology which generates extremely high
efficiencies due to the innovative particle design. This increase in
efficiency improves the resolution between critical oligo sequences,
gives higher sensitivity for easer MS quantitation, and allows for a
decrease in column length for higher throughput.
Core-Shell Technology for Synthetic DNA/RNA Analysis
Clarity Oligo-MS media is not fully porous like traditional particles
used for the analysis of oligonucleotides. It is a core-shell particle
technology which uses a sol-gel processing technique to grow a
homogeneous porous shell onto a solid core. This highly optimized
process combined with uniform particle size distribution produces
a column that generates extremely high plate counts.
Achieve Baseline Resolution of N-1 and N+1 Oligo

from Target
The high plate counts generated by the Clarity Oligo-MS material
produce extremely high efficiencies and thus excellent resolution between oligonucleotides of similar length and structure.
Scientists can achieve baseline resolution between synthetic
oligonucleotides with just one base difference allowing easier
quantitation.
Poly dT Standard (12-18mer)
Column:
Dimensions:
Part No.:
Mobile Phase:
Clarity 2.6 m Oligo-MS C18

50 x 2.0 mm
00B-4479-BO
A: 100 mM HFIP / 4 mM TEA / 2 % Methanol
B: 100 mM HFIP / 4 mM TEA / 98 % Methanol
A/B (95:5) to A/B (80:20) in 10 min
0.5 mL/min
50 C
UV @ 260 nm (22 C)
20 L
Poly dT (12-18)
Gradient:
Flow Rate:
Temperature:
Detection:
Injection Volume:
18836 Sample:
mAU
80
1.7 m
Core-Shell Particle
60
1.25 m Solid Core
0.23 m Porous Shell
40
App ID 18836
Boost performance of existing sub-2 m methods
20
0
0
1.7 m
10
12
min
Deliver increased efficiency at reduced backpressures

for UHPLC performance on any system
1.9 m Solid Core
0.35 m Porous Shell
Column:
Dimensions:
Part No.:
Mobile Phase:
2.6 m
Gradient:
Flow Rate:
Temperature:
Detection:
Injection Volume:
Sample:
18837
mAU
50 x 2.1
100 x 2.1
1.7 m Oligo-MS C18
00B-4480-AN
00D-4480-AN
60
2.6 m Oligo-MS C18
00B-4479-AN
00D-4479-AN
40
50 x 4.6
100 x 4.6
00B-4479-E0
00D-4479-E0

Phase

50 x 2.0 mm
00B-4479-BO
A/B (95:5) to A/B (80:20) in 10 min
0.5 mL/min
50 C
UV @ 260 nm (22 C)
20 L
Crude DNA 30mer
App ID 18837
Clarity Oligo-MS HPLC Columns

Minibore Columns (mm)
Phases
Rapid Separation of Complex Oligo Samples

Due to the high resolving power of Clarity Oligo-MS columns,
high-throughput methods for the separation of complex synthetic
mixtures can be developed. Using short (50 mm length) columns,
impurities are separated from the peak of interest in less than 12
minutes.
Crude DNA 30mer
clarity oligo-ms
2.6 m
Core-Shell Particle
uHPLC |
20
0
2.6 m Oligo-MS C18
Phenomenex
10
12 min
275
SecurityGuard ULTRA
If SecurityGuard Ultra cartridge

protection system does not perform as
well or better than your current guard
cartridge system of similiar phase and
dimensions, return the product with the
FULL REFUND

System for Any Sub-2 m
Column
Increases column lifetime of virtually all manufacturers
UHPLC, sub-2 m, and core-shell columns
Holder with cartridge
Offers more reproducible chromatography

For pressures up to 20,000 psi (1,378 bar)
Protects with No Loss of Column Performance!

With SecurityGuard Ultra, contaminants and microparticulates
are trapped within the guard cartridge instead of in your expensive
UHPLC column. The extremely low dead volume (<0.3 L) of this
unique guard design minimizes sample peak dispersion to maintain
column performance without altering your chromatography results.
Cartridge
Holder
1.7m Kinetex column with and without the SecurityGuard ULTRA cartridge system
19860
19859
With SecurityGuard Ultra
Without SecurityGuard Ultra

4
4
80
80
60
60
SecurityGuard ultra
20
App ID 19859
App ID 19860
0.0
40
uHPLC
40
20
0.2
0.4
0.6
0.8
1.0
1.2
1.4
min

Guard Cartridge: SecurityGuard Ultra C18 (ODS) 2.1 mm ID
Part No.: AJ0-8768
Parameters
With
SG ULTRA
Without
SG ULTRA
Difference
Selectivity
Efficiency (Plates/Meter)
Backpressure
1.36
237,220
360
1.35
246,080
348
-0.99%
-3.60%
3.45%
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
min
Sample: 1. Uracil
2. Acetophenone
3. Toluene
4. Naphthalene
For more about SecurityGuard Ultra and cartridge holder ordering information, see p. 239.
For Kinetex SecurityGuard Ultra cartridges, see p. 271
276
Phenomenex
BioSeparations
Bioseparations
Amino Acid Analysis

EZ:faast.................................................................................................. 278
Proteins/Peptides
Aeris....................................................................................................... 283

BioSep................................................................................................... 292
Jupiter.................................................................................................... 296
Synthetic DNA/RNA
Clarity..................................................................................................... 301
277 - 310
EZ:faast Amino Acid Analysis
U.S. Patent No. 6, 770, 246
Amino Acid Analysis

in 15 Minutes
Easy-to-use kit contains everything needed for sample
clean up, derivatization, and analysis of 384 samples
Effective Sample Clean Up
Kits compatible with GC/FID, GC/MS, & LC/MS

chromatographic systems
EZ:faast eliminates nearly all potential contaminants. The SPE and

liquid/liquid extraction steps remove a majority of the interfering
components. Additionally with the GC Kit, discrimination for nonvolatile contaminants then occurs at the GC injection port. These
discriminatory steps help produce chromatograms without interfering peaks from complex matrixes such as plasma and urine.
Simple & fast sample prep, derivatization, analysis

for both physiological (free) amino acids and protein
hydrolysates
BIOSEPARATIONS | amino acid analysis |
EZ:faast
Analyze amino acids from the most complex matrixes

such as blood, plasma, foodstuffs, and fermentation broth
No plasma protein or urea removal required for
physiological samples
Great sample purity and improved reproducibility
Method can be optimized for sulfur-containing and
aromatic amino acids, along with numerous other amino
acid derivatives
Without
De-proteinization
EZ: faast method
Performance Characteristics
Precision
EZ:faast allows for the quantitation of amino acids in various sample matrices with good precision. RSD values are listed for most
applications shown in this section of the catalog. These values are
better than 5 % for most amino acids and sample types, except for
ASN, GLN, HIS, ILE, MET, TRP and TYR with % RSD<12. RSD %
values include variation due to both sample preparation and analysis, other methods usually only consider analysis.
Accuracy
Recovery of EZ:faast varies between 89-107 %. An example of %
recovery for spiked amino acids in specific matrixes is shown below. Clover honey was used to demonstrate accuracy. Five samples were taken from a well-homogenized honey and tested for %
recovery (accuracy), each sample was spiked with 200 nmols/ mL
of five different amino acids. All data reported in nmol/mL.
ALA
ASN
PHE
LYS
TYR
Standard
Added
Honey +
Standard
Standard
Recovered
% Recovery
(Accuracy)
199
209
197
188
200
280.11
360.48
248.46
239.50
208.11
201.74
187.42
185.76
180.05
182.33
101.4
89.7
94.3
95.8
91.2
Five spiked samples showed good recovery (89.7 - 101.4 %) for

all amino acids analyzed. For another detailed accuracy example,
please refer to Technical Note (TN-8001) Asparagine Analysis in
Food Products.
Sensitivity
Limits of detection (LOD) are shown for each analytical technique
(GC/FID, GC/MS and LC/MS) in the lists of amino acids amenable
for analysis by EZ:faast. LODs were determined for the amino
acids included in the standard mixtures provided with the kit for
calibration purposes.
278
Table 1: Common Protein Removal Protocols vs. EZ:faast
Comparative data showing amino acid concentrations in mol/L from three common
deproteinized plasma samples (SSA = sulfosalicylic acid; TCA = trichloroacetic acid;
ORG = acetonitrile:ethanol 2:1) with a plasma sample analyzed by the GC/FID EZ:faast
method. The comparative data (mean values and ranges for 12 measurements) show
no significant differences between samples prepared by common protein removal
procedures or by the EZ:faast method.
GLY
ALA
ABA
LEU
ILE
MET
PRO
ASP
290
421
23
165
74
30
209
18
(286-293)
(415-427)
(22-24)
(162-168)
(72-75)
(29-30)
(207-211)
(17-19)
SSA
(Recommended for
OPA-derivatized
samples)
288
422
23
164
70
32
207
16
(282-293)
(417-427)
(20-26)
(162-166)
(69-72)
(31-33)
(204-210)
(15-17)
259
380
22
162
71
31
212
16
TCA
ORG
(Recommended for
PITC-derivatized
samples)
(238-280)
(357-402)
(21-22)
(158-165)
(69-72)
(30-31)
(208-215)
(14-17)
261
393
22
163
73
30
206
19
(251-270)
(365-421)
(21-23)
(155-170)
(72-73)
(29-30)
(197-214)
(18-20)
Derivative Stability
EZ:faast amino acid derivatives are stable at room temperature for
more than 24 hours. Samples prepared during the day can be left
on the autosampler tray at room temperature for analysis during the
night or the next day. Prepared samples can be stored for a couple
of days refrigerated or frozen for longer periods of time. The stability
of amino acid derivatives prepared with standard solutions passed
through the EZ:faast sample preparation procedure was tested at
different moments in time over a 19 hour period starting shortly after sample preparation. The first injection was used for instrument
calibration. Average % RSD for 22 amino acids was 2.9. Glycine
and Isoleucine showed minimal variation in response with 0.69 and
0.7 % RSD, respectively. HYP with RSD 11.07 % is the least stable
amino acid derivative.
Robustness
Method of analysis for amino acids based on the EZ:faast procedure is robust. Results are unaffected by most deviations from the
preferred sample preparation and analysis protocol. The following
parameters have been evaluated for their effect on method robustness: GC instrument settings, maximum loading capacity of sorbent
tips, sample pH, sample loading speed during SPE clean up, and
reaction times allowed for derivatization. The only critical parameters to monitor are speed of sample load during the SPE step and
minimum reaction time allowed for derivatization (total 3 min).
Phenomenex
U.S. Patent No. 6, 770, 246
Analyze Over 60 Amino Acids

with Several Kit Options
Based on Sample Type
Free (Physiological) Amino Acids
The EZ:faast kits for free amino acids provide rapid clean up, derivatization, and analysis of amino acids from complex mixtures,
while yielding a full amino acid profile in 15 minutes. The sample
preparation cleans up amino acids from complex matrixes like
blood, urine, cheese, cell cultures, and wine. Samples are precolumn derivatized to form stable amino acid derivatives.
Amino Acids Analyzed by GC

LOD* (nmol/mL) S/N 3:1
Chemical Name
Alanine
-Alanine
Alliin
-Aminoadipic acid
4-Aminobenzoic acid
-Aminobutyric acid
-Aminoisobutyric acid
-Amino-n-butyric acid
-Amino-n-butyric acid
-Aminopimelic acid
Arginino-succinic acid
Asparagine
Aspartame
Aspartic acid
Bicin
Abbreviation
FID
MS
ALA
-ALA
0.1
AAA
PABA
ABA
-AiB
ABA
GABA
APA
ARG-SUC
ASN
0.2
1
4
0.2
0.2
ASP
0.47
0.4
2.5
0.87
0.1
* LODs were determined for amino acids included in standard mixtures

provided with the kit
Note: several amino acids coelute under the chromatographic conditions
specified in the user manual
Phenomenex
LOD* (nmol/mL) S/N 3:1

Chemical Name
Carboxymethyl-cysteine
Chloro-phenylalanine
Cystathionine
Cysteine
Cystine
2,4-Diamino-n-butyric acid
Diaminopimelic
3,4-Dihydroxyphenylalanine
Dopamine
Ethanolamine
Ethionine
Fluoro-alanine
Glutamic acid
Glutamine
Glycine
Glycine-glycine (dipeptide)
Glycine-proline (dipeptide)
Histamine
Histidine
Homocysteine
Homocystine
Homophenylalanine
Homoserine
Hydroxylysine (2 isomers)
3-Hydroxyproline
4-Hydroxyproline
Isoleucine
allo-Isoleucine
Leucine
Lysine
Lysine-alanine (dipeptide)
Methionine
Methionine Sulfone
Methionine Sulfoxide
3-Methyl-cysteine
Naphthyl-alanine
3-Nitrotyrosine
Norleucine
Norvaline
Ornithine
Phenylalanine
Phenyl Glycine
Pipecolic Acid
Proline
Proline-hydroxyproline
(dipeptide)
Sarcosine
Seleno Cystine
Seleno Methionine
Serine
Serotonin
Theanine
Thioproline
Threonine
Threonine-aspartic acid (dipeptide)
Tryptophan
Tyramine
Tyrosine
Valine
Abbreviation
Cl-PHE
CTH
CYS
C-C
DABA
DAPA
DOPA
DA
FID
MS
10
10
2
8
2
0.2
10
0.1
0.2
10
2
0.65
0.65
0.65
1
0.2
0.2
0.1
0.1
0.2
0.87
0.2
1
0.47
0.2
0.2
1
0.87
0.1
10
0.1
0.2
ETH
GLU
GLN
GLY
GLY-GLY
GPR
HA
HIS
HCYS
HC-CH
HPHE
HSER
HLY
3HYP
4HYP
ILE
aILE
LEU
LYS
LYS-ALA
MET
NLE
NORV
ORN
PHE
PHE-GLY
HPRO
PRO
PHP
SAR
Se-C-C
Se-MET
SER
SRO
THE
TPR
THR
THR-ASP
TRP
0.43
2
0.1
0.2
0.43
0.1
TYR
VAL
0.4
0.6
0.2
0.2
EZ:faast | amino acid analysis | BIOSEPARATIONS
Protein Hydrolysates
Within 15 minutes, hydrolyzed protein or peptide samples are prepared and derivatized for rapid sequencing. Sample preparation
ensures non-hydrolyzed protein removal for more accurate analysis. Derivatized samples will not degrade rapidly.
Amino Acids Analyzed by GC (cont'd)
279
U.S. Patent No. 6, 770, 246
Analyze Over 60 Amino Acids

with Several Kit Options (contd)
EZ:faast
Based on Chromatographic Instrument

Gas Chromatography (GC)
The GC kit options are compatible with a NPD, FID, or MS detectors. The analysis time on the GC column is a quick 8 minutes and
provides excellent resolution of all amino acids in the profile. The
derivatization procedure used makes the amino acids less polar
and therefore more volatile so they can be analyzed via GC. Due to
the fact that Arginine and Citrulline are still very polar after derivatization, they get stuck in the GC liner and cant be analyzed on the
GC. However, they can be analyzed with the LC kits. The complete
cycle time using the GC kit options is 15 minutes.
Liquid Chromatography (HPLC)
LC/MS kit options are available for labs without GC systems or to
analyze Arginine or Citrulline. The sample preparation and derivatization is still just 7 minutes, but the analysis is slightly longer at
12 minutes. The derivatization procedure offers a slightly different
benefit for LC than for GC. Through derivatization, the molecular
weight of the amino acids is significantly increased moving the
scanning range and giving a much more favorable signal to noise
ratio and minimizing interferences. The LC kit options give a complete amino acid profile, starting from raw sample, in 19 minutes.
Amino Acids Analyzed by LC/MS

Chemical Name
Adrenaline
Alanine
-Alanine
Alanyl-alanine (dipeptide)
-Aminoadipic acid
4-Aminobenzoic acid
-Aminobutyric acid
-Aminobutyric acid
4-Aminobutyric acid
-Aminoisobutyric acid
Aminopimelic acid
Anserine
Arginine
Arginino-succinic acid
Asparagine
Aspartame
Aspartame acid
Aspartic acid
Abbreviation
LOD* (pmol/mL)
S/N=3
ALA
bALA
ALA-ALA
AAA
PABA
ABA
bABA
GABA
bAiBA
APA
ANS
ARG
20
ASN
10
ASP-PHE
ASP
1
5
5
5
2
2
* LODs were determined for amino acids included in the standard

mixtures provided with the kit
** extended gradient time required
*** alternative chromatographic conditions required
280
Amino Acids Analyzed by LC/MS (cont'd)

Chemical Name
Carboxymethyl-cysteine
Carnosine
Citrulline
Cystathionine
Cysteine
Cysteine-Homocysteine (dipeptide)
Cystine
2,4-Diaminobutyric acid
Dimethylarginine***
Dimethylarginine***
Dopamine
Ethanolamine
Ethionine
Glutamic acid
Glutamine
-Glutamyl-e-lysine (dipeptide)
Glycine
Glycine-proline (dipeptide)
Histamine
Histidine
Homoarginine
Homocysteine
Homocystine**
Homophenylalanine
Homoserine
-Hydroxylysine
4-Hydroxyproline
3-Hydroxy-tyrosine**
Isoleucine
allo-Isoleucine
Leucine
Lysine
Lysine-alanine (dipeptide)
Lysinoalanine
Methionine
Methionine-d3
Methionine sulfone
Methionine sulfoxide
Methionine sulfoximine
1-Methyl-histidine
3-Methyl-histidine
Norleucine
Ornithine
Phenylalanine
Pipecolic acid
Proline
Proline-hydroxyproline (dipeptide)
S-Pyridylethyl-cysteine
Pyroglutamic acid
Sarcosine
Seleno-cystine**
Seleno-methionine
Serine
Theanine
Thiaproline
Threonine
Tryptophan
Tyrosine
Valine
Abbreviation
CIT
CTH
CYS
C-HC
C-C
DABA
SDMA
ADMA
DA
ETH
GLU
GLN
GLU-LYS
GLY
GPR
HA
HIS
HARG
HCYS
HC-CH
HPHE
HSER
HLY
HYP
DOPA
ILE
aILE
LEU
LYS
LYS-ALA
LAL
MET
Met-d3
1MHIS
3MHIS
NLEU
ORN
PHE
HPRO
PRO
PHP
pGLU
SAR
Se-C
Se-MET
SER
THE
TPR
THR
TRP
TYR
VAL
LOD* (pmol/mL)
S/N=3
1
5
5
1
1
NA
NA
100
5
5
2
0.1
1
NA
5
5
1
1
1
2
10
5
10
10
2
10
1
Phenomenex
U.S. Patent No. 6, 770, 246
Medical and Clinical

Toxicology Solutions
Biotechnology and
Pharmaceutical Solutions
Analyze complex blood, urine, and plasma samples
For the analysis of amino acids in fermentation broths,

cell cultures, serum, and protein hydrolysates
Monitor metabolic diseases such as

Phenylketonuria (PKU)
High-throughput screening of biological lab samples
Rapid 7- minute clean up of proteins, salts, and
other contaminants
Elimination of problems with urea or other contaminant
interference during analysis
On-line analysis speed with analytical precision for

monitoring amino acid depletion in fermentation broths
and cell cultures
Easy method of determining composition and
concentration of proteins
Identify the modification and mis-incorporation of amino
acids in proteins
PKU (Phenylketonuria) Screening

Kit:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
EZ:faast | amino acid analysis | BIOSEPARATIONS
Rapid 7- minute clean up of proteins, salts, and other

contaminants
Mammalian Cell Culture
Kit: EZ:faast GC/FID
Free (Physiological) Amino Acid Kit
Part No.: KG0-7165
Injection: Split 1:15 @ 250 C, 2 L
Carrier Gas: Helium 1.5 mL/ minute, constant flow @ 110 C
Oven Program: 30 C/ min from 110 C to 320 C, hold at 320 C for 1 minute
Sample: Derivatized amino acids in mammalian cell culture (0.1 mL). Norvaline
is the internal standard added at a concentration of 200 mol/L.
3. Alanine (248, 2.1)*
13. 4-Hydroxyproline (45, 0.9)*
4. Glycine (304, 2.9)*
14. Glutamic Acid (538, 5.6)*
5. Valine (685, 3.8)*
15. Phenylalanine (50, 2.3)*
6. Norvaline (IS)
16. Glutamine (105, 10.5)*
7. Leucine (149, 2.2)*
17. Ornithine (93, 3.8)*
8. Isoleucine (138, 8.6)*
18. Glycine-proline (dipeptide) (57, 5.6)*
9. Threonine (891, 3.6)*
19. Lysine (134, 5.5)*
10. Serine (124, 4.8)*
20. Histidine (168, 10.5)*
11. Proline (1220, 2.3)*
21. Tyrosine (45, 9.2)*
12. Asparagine (630, 2.6)* 22. Tryptophan (462, 11.5)*
EZ:faast GC/FID Free (Physiological) Amino Acid Kit

KG0-7165
Split 1:15 @ 250 C, 2 L
Helium 2 mL/ minute constant flow @ 110 C
30 C/ min from 110 C to 320 C, hold at 320 C for 1 minute
FID @ 320 C
1. Aspartic Acid (10, 4.3)*
7. Lysine (52.5, 5.4)*
2. Methionine (12.5, 9.6)*
8. Histidine (20, 10.3)*
3. 4-Hydroxyproline (22.5, 3.5)*
9. Norvaline
4. Glutamic Acid (337.5, 2.8)*
10. Tyrosine (35, 11.1)*
11. Tryptophan (7.5, 11.6)*
6. Ornithine (32.5, 3.6)*
* Detection Limit (nmol/mL), RSD (n=30) %
14911
5
200
pA
App ID 14911
100
4
12 3
10
11
0
1
14910
min
11
300
200
5
22
9
10
100
3 4
12
14
8
15
13
16
17
18
19 20
21
App ID 14910
pA
0
1
Phenomenex
min
281
Evaluate EZ:faast in your lab for 45

days, if you are not completely satisfied
return it for a FULL REFUND.
U.S. Patent No. 6, 770, 246
Food and Beverage Solutions

Analyze complex wine, beer, juice, foodstuffs,
fermentation broths, cell culture, and protein hydrolysate
matrices
Quantitative tool and stability assessment for amino acids
added to food products
Analyze the amino acid composition of foods and
beverages
A tool for quick QC testing of amino acid content of a
finished product
Free Amino Acids in Beer

Kit:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
EZ:faast
Rapid 7- minute clean up of proteins, salts, and other

contaminants
Theanine Analysis in Tea
Column:
Part No.:
Injection:
Carrier Gas:
Oven Program:
Detector:
Sample:
EZ:faast GC/FID Free (Physiological) Amino Acid Kit

KG0-7165
Split 1:15 @ 250 C, 2 L
Helium 1.1 mL/ minute constant flow @ 110 C
30 C/ min from 110 C to 320 C, hold at 320 C for 1 minute
FID @ 250 C
Derivatized amino acids in beer. Norvaline is the internal
standard at 200 mol/L
1. Alanine (803, 2.5)*
10. Aspartic Acid (37, 2.6)*
2. Glycine (262, 3.4)*
11. Glutamic Acid (75, 5.3)*
3. Valine (203, 3.4)*
4. Norvaline (IS)
13. Ornithine (76, 3.3)*
5. Leucine (49, 2.6)*
14. Histidine (100, 10.8)*
6. Isoleucine (23, 7.9)*
15. Tyrosine (207, 10.5)*
7. Serine (754, 5.6)*
16. Tryptophan (119, 11.3)*
8. Proline (3908, 2.8)*
9. Asparagine (35, 10.1)*
EZ:faast GC/MS Free (Physiological) Amino Acid Kit

KG0-7166
Split 1:15 @ 250 C, 2 L
Helium 1.1 mL/ minute constant flow @ 110 C
32 C/ min from 110 C to 320 C
MS @ 45-450 m/z
1. Alanine
10. Glutamic Acid
2. Valine
11. Phenylalanine
3. Norvaline (IS)
12. Theanine & Glutamine
4. Leucine
13. Tyrosine
5. Isoleucine
14. Tryptophan
6. Serine
7. Proline
8. Asparagine
9. Aspartic Acid
14623
8
16002
12
12
56
10
3
13
11
4
14
16
9
1
1.5
min
10
11
8
45 67
2
2.5
3.5
13
4
4.5
14
5.5
App ID 16002
15
App ID 14623
min
Amino Acid Analysis Kits
Each kit includes: ZB-AAA GC column, or AAA LC column, sample prep and derivatization reagents, sample prep vials, AA
standards, SPE pipette tips, vial rack, autosampler vials with inserts with MS kits, and microdispenser.
Part No.
KG0-7165
KG0-7166
KG0-7167
KG0-7168
KH0-7337
KH0-7338
KH0-7339
KH0-7340
AG0-7184
AG0-7263
AL0-7500
AL0-7501
282
Description
GC/FID Free (Physiological) Amino Acid Analysis Kit
GC/MS Free (Physiological) Amino Acid Analysis Kit
GC/FID Protein Hydrolysate Kit
GC/MS Protein Hydrolysate Kit
LC/MS Free (Physiological) Amino Acids Kit with 250 x 2.0 mm column
LC/MS Free (Physiological) Amino Acids Kit with 250 x 3.0 mm column
LC/MS Protein Hydrolysates Kit with 250 x 2.0 mm column
LC/MS Protein Hydrolysates Kit with 250 x 3.0 mm column
GC Free (Physiological) Amino Acid Standards (SD1, 2, 3) 2 mL/vial x 2
GC Protein Hydrolysate Standard (SD) 2 mL/vial x 2
LC/MS Free (Physiological) Amino Acid Standards (SD1, 2, 3) 2 mL/vial x 2
LC/MS Protein Hydrolysate Standard (SD)2 mL/vial x 2
Unit
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
Price
Phenomenex
6.5
min
Aeris

FULL REFUND.
Core-Shell HPLC/UHPLC Columns

for Proteins and Peptides
Ultra-High Resolution and Performance
Introducing Aeris, a specialized line of reversed phase core-shell
HPLC / UHPLC columns, built exclusively for the ultra-high performance separation and analysis of proteins and peptides.
These columns can provide improved resolving power, selectivity,

throughput, sensitivity, column lifetime, and method flexibility compared to other fully porous and core-shell columns typically used
for bioseparations.
Aeris PEPTIDE
3.6 m
3.6 m
1.7 m
Large pore optimized for intact

proteins3.6---3.2
and
polypeptides
solid core
1.7---1.25
solid core
3.6---3.2
solid core
Small pore optimized for peptides

and 3.6---2.6
for peptide
mapping 1.7---1.25 solid core
solid core
The precise architecture of Aeris core-shell particles provides dramatic leaps in performance in two
important ways:
The thin, porous layer, or shell, decreases the diffusion

path length, thus reducing the time it takes for biomolecules
to adsorb/desorb into and out of the particle.
Aeris Core-Shell Particle
Uniform sizing and shape of the particles along with tight

packing specifications reduces losses in efficiency and performance due to band broadening.
Aeris Core-Shell Particles
aeris | proteins/peptides | BIOSEPARATIONS
Aeris WIDEPORE
Band Broadening
Band Broadening
Fully Porous Particles
Fully Porous Particle
Band Broadening
Band Broadening
The result is:

3.6m core-shell particles that can perform like sub-2m
columns on both HPLC and UHPLC systems at a fraction of
the pressure
Phenomenex
1.7m core-shell particles that can provide higher peak

capacities compared to fully porous sub-2m columns on
UHPLC systems
283
Aeris
Selecting the Optimal Aeris

Column for Your Applications
PROTEIN
OR
PEPTIDE
Aeris core-shell columns are designed for the separation of complex protein and peptide mixtures. Chromatographers can easily
narrow down the column(s) that has a high probability of success
for their separation by selecting from a variety of phase, pore size,
and particle size options.
> 10kDa
< 10kDa
Aeris PEPTIDE
Recommended for the separation of low molecular weight
peptides and for peptide mapping.
UHPLC
or
HPLC
SYSTEMS
BIOSEPARATIONS | proteins/peptides | aeris
XB-C18 chemistry best suited for resolving peptides

1.7m and 3.6m particles for method development
flexibility
Small pore optimized for peptide diffusion
Aeris WIDEPORE
Hydrophilic
Proteins
Recommended for the separation of intact proteins and

polypeptides.
XB-C18, XB-C8, and C4 phases for alternate
selectivities
Aeris WIDEPORE
3.6m XB-C18
3.6m particle for system flexibility

Large pore optimized for fast protein adsorption/
desorption
Hydrophobic
Proteins
HPLC
SYSTEM
UHPLC
SYSTEM
Aeris PEPTIDE
3.6m XB-C18
Aeris PEPTIDE
1.7m XB-C18
Aeris PEPTIDE
3.6m XB-C18
1.7 m or 3.6 m on your
UHPLC system?
Go to p. 287 to decide!
Aeris WIDEPORE
3.6m XB-C8
Very
Hydrophobic
Proteins
Aeris WIDEPORE
3.6m C4
Packing Material
Total Particle Size

(m)
Porous Shell
(m)
Core Size
(m)
pH
Stability
Temp
Stability
Pressure
Stability
Aeris WIDEPORE
Aeris PEPTIDE
Aeris PEPTIDE
3.6
1.7
3.6
0.2
0.22
0.5
3.2
1.25
2.6
1.5 - 9
1.5 - 9
1.5 - 9
90C
90C
90C
600bar
1000bar
600bar
Aeris WIDEPORE XB-C18 and Aeris PEPTIDE XB-C18 make a

perfect pair for peptide mapping. See p. 287 for more details.
284
Phenomenex
Aeris
Improve Resolution on ANY System

by Leveraging Low Backpressure
Aeris PEPTIDE and Aeris WIDEPORE 3.6m columns can perform
like sub-2m columns at a fraction of the backpressure. This allows
chromatographers to utilize the resolving power of longer length
(or coupled) columns without exceeding the pressure limits of their
HPLC system. Scientists analyzing proteins and peptides can now
have ultra-high resolution on HPLC or UHPLC systems.
Sub-2m Performance at a Fraction of the Backpressure
AppID 19826
19818
mAU
mAU
30
UHPLC Results at
1/4 the Backpressure
485bar
Peak width: 0.041min
No. of peaks: 50
40
30
App ID 19818
10
0
App ID 19826
20
20
10
0
-10
-10
0
10
15
min
20

Column: Aeris WIDEPORE 3.6m XB-C18
ACQUITY BEH 300 1.7m C18
Gradient: A/B (65:35) for 3min to A/B (35:65) over 30min
Flow Rate:
Temperature:
Injection Volume:
Instrument:
Detection:
Sample:
Increase Column Length to Improve Resolving Power
10
App ID 19855

mAU
mAU
min
20
0.3mL/min
40C
20L
Agilent 1200
BSA (Bovine Serum Albumin) Tryptic Digest
19843
15
150 x 2.1mm
142bar
40
250 x 2.1mm
120bar
Peak width: 0.046min
No. of peaks: 49
40
220bar
40
30
30
10
App ID 19843
10
App ID 19855
20
20
-10
-10
5
10
15
20
25 min
App ID 19854
App ID 19856
mAU
10
mAU
10
4
2
-2
-4
App ID 19854
10
15
20
25
30
min
Longer Column =
Increased Resolution
4
2
0
-2
-4
App ID 19856

Temperature:
Dimensions: as noted
Injection Volume:
Instrument:
Detection:
Sample:
Flow Rate: 0.3mL/min
40C
25L
Agilent 1200
BSA (Bovine Serum Albumin) Digest
Waters and ACQUITY are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Phenomenex is not affiliated with Waters Corporation. Studies were performed using new columns and, to the
Phenomenex
285
Aeris
Long Column Lifetimes

Under Extreme Method Conditions
Low Column Bleed

for Amplified Mass Spec (MS) Sensitivity
Aeris columns provide temperature stability up to 90C, and

pH stability from 1.5 - 9, giving ample flexibility for method
development and excellent column lifetime.
Aeris columns show no significant phase bleed under LC/MS

conditions, making them very suitable for protein and peptide
analysis. Chemists can be assured accurate, dependable, and
consistent results, time and time again.
19832
Over 1,000 Injections at 90C
Virtually no LC/MS Bleed

19831
mAU
20
Column Generates Little to

No MS Interference
7.0e7
6.0e7
Intensity, cps
App ID 19832
App ID 19833
No Loss in
Performance
40
5.0e7
4.0e7
3.0e7
2.0e7
App ID 19831
8.0e7
1.0e7
10
Column:
Dimensions:
Part No.:
Mobile Phase:

50 x 4.6mm
00B-4282-E0
A: Water with 0.1% TFA
Gradient: A/B (97:3) for 3min, then to
A/B (35:65) over 20min
15
Flow Rate:
Temperature:
Injection Volume:
Detection:
Sample:
min
0.0
0
Column:
Dimensions:
Part No.:
Mobile Phase:

Flow Rate:
50 x 2.1mm
Temperature:
00B-4282-AN
Detection:
A: Water with 0.1% Formic Acid
B: Acetonitrile with 0.1% Formic Acid
Gradient: A/B (95:5) for 2.5min, to A/B (5:95)
Sample:
hold for 0.5min, then re-equilibrate
1.5mL/min
90C
10L
Apomyoglobin Digest
min
0.5mL/min
25C
MS (API 4000)
Positive Ion Mode
Q1 scan from 75 to 800amu
Blank
Minimize Adsorption and Maximize Recoveries for Accurate Results

Aeris phase chemistries and bonding technology create a highly inert surface, leading to greatly reduced irreversible adsorption, higher
recoveries, and sharper, narrower peaks, providing high quality and accurate results for each consecutive analysis.
Maximize Recoveries of Hydrophobic Proteins
AppID 19838
Aeris WIDEPORE 3.6m C4
mAU
Agilent ZORBAX 300SB 3.5m C3
19839
mAU
80
80
60
Protein
Adsorbed
60
App ID 19838
40
20
App ID 19839
40
20
-20
16
18
20
22
24
26
28

Column: Aeris WIDEPORE 3.6m C4
ZORBAX 300SB 3.5m C3
Gradient: A/B (97:3) to A/B (35:65) over 45min
min
16
Flow Rate:
Temperature:
Injection Volume:
Instrument:
Detection:
Sample:
18
20
22
24
26
28
min
0.3mL/min
40C
20L
Agilent 1200
Human Epidermal Growth Factor
Agilent and ZORBAX are registered trademarks of Agilent Technologies, Inc. Phenomenex is not affiliated with Agilent Technologies, Inc. Studies were performed using new columns and, to the extent possible, identical
experimental conditions were applied. Comparative separations may not be representative of all applications
286
Phenomenex
Aeris
Aeris PEPTIDE Columns

for Peptide and Peptide
Mapping Separations
1.7m Core-Shell Particle

1.25m Solid Core
0.22m Porous Shell
Based on core-shell particle technology, Aeris PEPTIDE particles

are designed with small pores, inert XB-C18 surface chemistry, and
two different particle sizes (3.6m and 1.7m) to meet the resolution demands of chromatographers performing complex peptide
and peptide map separations on HPLC and/or UHPLC systems.
1.7m
Aeris PEPTIDE columns are built for

the following:
Synthetic peptide impurity analysis

2.6m Solid Core
Identifying protein modifications

Glycosylation, Substitution, and Truncation
0.5m Porous Shell
Analyzing post-translational modifications

Deamidation, Oxidation, and Deletions
3.6m
Select the Most Suitable Aeris

PEPTIDE Column to Achieve Your
Separation Goals
The family of Aeris PEPTIDE XB-C18 columns is designed to provide versatility for the development of peptide separation methods.
Depending on your resolution, throughput goals, and pressure
capabilities of your system, you can choose from two particle
sizes with unique performance attributes, as well as several column
lengths to select the most suitable column for seamless method
development and excellent results. 3.6---3.2 solid core
Resolution
Aeris PEPTIDE
1.7m
150 mm length
Aeris PEPTIDE
1.7m
100 mm length
Aeris PEPTIDE
1.7m
50 mm length
Aeris PEPTIDE
3.6m
50 mm length
Peptide mapping
Aeris PEPTIDE
3.6m
250 mm length
Aeris PEPTIDE
3.6m
150 mm length
Aeris PEPTIDE
3.6m
100 mm length
UHPLC system required
HPLC or UHPLC compatible
run time
Phenomenex
287
Aeris
Ultra-High Resolving Power on HPLC and UHPLC Systems

with Aeris PEPTIDE 3.6m Columns
The Aeris PEPTIDE 3.6m core shell column was designed with
one purpose in mind: to maximize the separation of large numbers
of peptides on any HPLC or UHPLC system. Because core shell
particles remove the backpressure constraints of HPLC or UHPLC
systems, chromatographers can achieve the ultra-high performance

of similar length sub-2m columns at a fraction of the backpressure.
UHPLC Performance at HPLC Compatible Backpressures
19930
19928

mAU
mAU
100
Resolves an Additional
Pair of Peptides
100
80
80
60
60
20
0
25
30
35
40
App ID 19930
40
40
App ID 19928
465bar
125bar
20
0
min
25

ACQUITY BEH 1.7m C18
30
Flow Rate:
Temperature:
Instrument:
Detection:
Sample:
35
min
40
0.3mL/min
40C
Agilent 1200SL
Human IgG Tryptic Digest
Utilize Long Columns to Maximize Separation Power

For applications like peptide separations and peptide mapping
where resolution is the primary goal, the lower backpressure of
Aeris PEPTIDE 3.6m core-shell columns allow one to use longer
columns for higher resolving power resulting in increased separation of closely eluting peptides. Use longer (or coupled) 3.6m
columns on UHPLC and HPLC systems to resolve critical peaks.
Minimal Increase in
Backpressure

19880
19882
140bar
150 x 4.6mm
mAU

200bar
250 x 4.6mm
mAU
30
10
10
11
12
min

Dimensions: as noted
Mobile Phase: A: Water with 0.1% Formic Acid
B: Acetonitrile with 0.1% Formic Acid
App ID 19882
App ID 19880
20
20
12
Temperature:
Injection Volume:
Instrument:
Detection:
Sample:
14
16
18
20
22 min
40C
25L
Agilent 1200
Bovine Serum Albumin (BSA) Tryptic Digest
Waters and ACQUITY are registered trademarks, and BEH Techonology is a trademark of Waters Corporation. Phenomenex is not affiliated with Waters Corporation. Studies were performed using new columns and, to the
288
Phenomenex
Aeris
Aeris WIDEPORE Columns for

Intact Protein and Polypeptide
Separations

0.2m Porous Shell
3.2m Solid Core
Aeris WIDEPORE columns are packed with 3.6m core-shell particles that are specially engineered with a thin porous shell, large
pores, and sterically protected XB surface chemistry to address the
inherent separation challenges of proteins and peptides. This unique
mix of features results in low backpressures, fast rates of diffusion,
and excellent selectivity, generating exceptional chromatographic
resolution on both HPLC and UHPLC systems.
3.6m
Recommended for:
Protein structural characterization
Post-translational modification identification
PEGylated proteins, antibodies, biogenerics, etc.
Impurity profiling
Peptide mapping
3.6---2.6 soli
Easy Method Development with Three Selectivities

Aeris WIDEPORE 3.6m Core-Shell Stationary Phases
TMS
Si
TMS
TMS
Si
TMS
TMS
TMS
XBC18
XBC8
C4
XB-C18
Maximum hydrophobicity
recommended for:
XB-C8
Moderate hydrophobicity
recommended for:
C4
Low hydrophobicity
recommended for:
Proteins
Large proteins
Very large proteins
Hydrophilic proteins
Moderately hydrophobic proteins
Very hydrophobic proteins
PEGylated proteins
Monoclonal antibodies
Membrane proteins
High temperature separations
Glycosylated proteins
Least retentive
Alternative selectivity for peptide

mapping
High temperature separations
Phenomenex
Stability indicating assays
289
Aeris
Maximize HPLC and UHPLC Resolving Power with Unique 3.6m Core-Shell Particle
3.6m core-shell technology combined with inert surface chemistries and tight packing specifications results in Aeris WIDEPORE
columns delivering exceptional resolving power at significantly
lower backpressures. Chromatographers now have the ability to
generate higher quality data than typically produced by columns

packed with fully porous particles for every protein analysis on
HPLC or UHPLC systems.
Performance Equivalent to Sub-2m Particle at Low Backpressure
19912

AppID 19838
mAU
80
80
60
App ID 19912
40
20
140bar
App ID 19838
420bar
60
UHPLC Results at
1/3 the Backpressure
mAU
40
20
0
16
18
20
22
24
26
28
16
min

Column: ACQUITY BEH 300 1.7m C18
Gradient: A/B (97:3) to A/B (35:65) over 45min
18
Flow Rate:
Temperature:
Injection Volume:
Instrument:
Detection:
Sample:
20
22
24
26
28
min
0.3mL/min
40C
10L
Agilent 1200
Human Epidermal Growth Factor (EGF)
Achieve UHPLC Performance on HPLC Systems by Replacing 3m and 5m Columns

The innovative structure of 3.6m Aeris core-shell particles was
specially designed to provide sub-2m performance at backpressures similar to fully porous 3m and 5m particles. Aeris columns
can deliver increased resolution for existing protein and peptide
separations performed on fully porous 3m and 5m columns,
using the same HPLC system!

Now you can have UHPLC performance on your HPLC system and
experience better performance and method flexibility than ever
before.
Upgrade Existing Methods on 3m and 5m Fully Porous Columns to Aeris Core-Shell Technology
Agilent ZORBAX 300SB 3.5m C18
19836
AppID 19837
mAU
mAU
20
150bar
15
15
10
App ID 19837
10
0
-5
-10
16
18
20
22
24
26
28
min
Dramatically Improved
Resolving Power
150bar
App ID 19836
20
0
-5
-10

ZORBAX 300SB 3.5m C18
16
18
Flow Rate:
Temperature:
Injection Volume:
Instrument:
Detection:
Sample:
20
22
24
26
28
min
1.5mL/min
40C
20L
Agilent 1200SL
Degraded Myoglobin
Agilent and ZORBAX are registered trademarks of Agilent Technologies, Inc. Phenomenex is not affiliated with Waters Coroation nor Agilent Technologies, Inc. Studies were performed using new columns and, to the extent
possible, identical experimental conditions were applied. Comparative separations may not be representative of all applications.
290
Phenomenex
Aeris

FULL REFUND.
Bundle Aeris PEPTIDE with

Aeris WIDEPORE for Detailed
Peptide Maps

00F-4482-E0
Temperature: 40C
Instrument: Agilent 1200SL
Sample: Alpha-1 Acid Glycoprotein Tryptic Digest
Aeris PEPTIDE 3.6m XB-C18 and Aeris WIDEPORE 3.6m XBC18 are a must-have pair for chromatographers who analyze
complex peptide mixtures. Because each has a unique pore size
and surface area, they exhibit different selectivity. Protein chemists
can take advantage of this diversity to achieve the critical resolution
of target peptides in various regions of the map, thus simplifying
their method development.
80
80
60
60
40
40
App ID 19929
mAU
20
App ID 19925
19925
19929
mAU
20
0
15
16
17
18
19
20
21
22
23
min
15
16
17
18
19
20
21
22
23
min
Utilize Differences in Small and Large Pore

Size Selectivity for Better Resolution
Phases
50 x 2.1
100 x 2.1
XB-C18
00B-4506-AN
00D-4506-AN

Phases
50 x 2.1
100 x 2.1
XB-C18
00B-4507-AN
00D-4507-AN
Phenomenex
00D-4507-E0
00F-4506-AN
150 x 2.1
250 x 2.1
00F-4507-AN
00G-4507-AN
Aeris PEPTIDE 3.6 m Analytical Columns (mm)

Phases
100 x 4.6
150 x 4.6
XB-C18
150 x 2.1
00F-4507-E0
250 x 4.6
Aeris WIDEPORE 3.6 m Minibore Columns (mm)

Phases
50 x 2.1
100 x 2.1
150 x 2.1
250 x 2.1
XB-C18
00B-4482-AN
00D-4482-AN
00F-4482-AN
00G-4482-AN
XB-C8
00B-4481-AN
00D-4481-AN
00F-4481-AN
00G-4481-AN
C4
00B-4486-AN
00D-4486-AN
00F-4486-AN
00G-4486-AN
Aeris WIDEPORE 3.6 m Analytical Columns (mm)

Phases
100 x 4.6
150 x 4.6
250 x 4.6
XB-C18
00D-4482-E0
00F-4482-E0
00G-4482-E0
XB-C8
00D-4481-E0
00F-4481-E0
00G-4481-E0
C4
00D-4486-E0
00F-4486-E0
00G-4486-E0
00G-4507-E0
291
BioSep -SEC-S
If BioSep analytical columns do not

provide you with at least an equivalent
separation as any other GFC column of
similar porosity, type and dimensions,
Gel Filtration Chromatography is used to analyze and/or characterize

proteins, peptides and other biomolecules; including antibodies,
immunoglobulins, protein complexes, protein aggregates, and
desalting. BioSep GFC columns offer many important benefits for
your separation needs.
Hydrophilically bonded rigid spherical silica gel has nominal

adsorbtion and high sample recoveries
18897
Batch-to-batch and column-to-column reproducibility is imperative.

Each batch of BioSep media is carefully monitored and QC tested
to ensure proper size, shape and pore characteristics, ensuring the
same high quality separation time and time again.
mAU
Narrow defined pore size distribution produces exceptional

separations for SEC
Optimized packing specifications provide high resolution,

maximized efficiencies, and exceptional peak asymmetry
S-2000 200 - 75,000
102
103
App ID 18897
2
1
0
S-4000 15,000 - 500,000

S-3000 5,000 - 100,000
104
105
Molecular Weight (Daltons)
50
S-4000 15,000 - 1,500,000

S-3000 5,000 - 700,000
S-2000 1,000 - 300,000
Native
3 Separate
Batches Overlaid
100
S-4000 5,000 - 700,000

S-3000 1,000 - 150,000
S-2000 500 - 100,000
Under
0.5 % SDS
150
Molecular Weight Separation Range for Proteins on BioSep-SEC-S

3 BioSep phase options to separate samples of varying molecular
weight (MW) ranges: 2000, 3000, 4000, as described below:
Under
6 M GnHCI
200
App ID 18927
BIOSEPARATIONS | proteins/peptides | BioSep-SEC-S
Aqueous Size Exclusion (SEC)/

Gel Filtration (GFC) for Protein
and Peptide Analysis
106
107
High Efficiencies for Greater Resolution

Due to a tight particle size distribution and a specialized packing
process and specifications, you will see greater baseline separation
between your analytes.
10
12
14
min
Highly Inert Material for Better Recovery

and Quantitation
BioSep columns experience a nominal amount of non-specific interactions which provides an extremely inert media demonstrating
18966
distinct
advantages for accurate quantitation of your proteins and
aggregates.
Human Serum under Different Mobile Phases
Efficiency
(minimum number theoretical plates on 300 x 7.8 mm column)
SEC-s2000
SEC-s3000
SEC-s4000
30,000
30,000
25,000
Plates
2. Mobile phase containing Arginine
Plates
App ID 18966
Plates
1. Mobile phase containing NaCl
3. Mobile phase without Arginine
10
12
14
min
Equal recovery of proteins and aggregates under different mobile phase conditions is
indicative of a highly inert column.
292
Phenomenex
BioSep -SEC-S
Low MW Proteins and Peptides on

BioSep-SEC-s2000
Medium MW Proteins on
BioSep-SEC-s3000
BioSep-SEC-s2000 columns are used for peptide therapeutics,

small proteins, PEGylated peptides and small PEGylated proteins, as well as biogeneric aggregate applications.
BioSep-SEC-s3000 columns are great for medium to large

MW proteins, serum proteins, immunoglobulins, and aggregate
applications.
Low MW Protein and Peptide Mixture
Protein Mixture
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
18945
Sample:
Column:
Dimensions:
18928
Part No.:
Mobile Phase:
12. Bradykinin 1-5
573 Da
11. Bradykinin 1-7
760 Da
10. Angiotensin 1-7
899 Da
9. Angiotensin II
1046 Da
8. Bradykinin
1060 Da
7. Substance P 1-9
1104 Da
6. Angiotensin I
1297 Da
5. Glucagon
3482 Da
4. Insulin
5700 Da
3. Apotinin
6500 Da
2. Cytochrome c
12400 Da
1. Myoglobin
17800 Da
10
min
TSKgel is a registered trademark of Tosoh Corporation. Phenomenex is in no way affiliated with Tosoh
Corporation. Comparative separations may not be representative of all applications.
Protein Separation of 50-300,000 MW on BioSep-SEC-s2000

vs. TSKgel G2000SWXL
Columns: BioSep-SEC-s2000
TSKgel G2000SWXL
18892 Phase: 10mM Tris pH 7.4,
Mobile
150mM Sodium Chloride
Detection: UV @ 214nm
Sample: 1. Hu IgA 300 kDa
2. b-Amylase 200 kDa
3. BSA 66 kDa
4. Ovalbumin 45 kDa
mAU
10
12
min
High MW Protein Mixture

Column:
Dimensions:
Part No.:
Mobile Phase:
18936
2
400
1
4
BioSep-SEC-s4000
300 x 7.8mm
00H-2147-K0
100mM Sodium Phosphate pH 7.0,
1mL/min
Ambient
UV @ 214nm
1. HMW impurity
2. lgM 900 kDa
3. Thyroglobulin 669 kDa
4. lgA 300 kDa
6. BSA 66 kDa
7. Ribonuclease A 13.7 kDa
8. Uridine 244 Da
Co-migration
of peaks 1 & 2
3
3
TSKgel
G2000SWXL
App ID 18892
1,2
200
100
BioSep-SEC-s4000 is capable of resolving large MW proteins

and PEGylated IgG applications.
BioSep-SEC-s2000
All four proteins

are resolved
Large MW Proteins on
BioSep-SEC-s4000
Flow Rate:
Temperature:
Detection:
Sample:
300
App ID 18928
358 Da
5
3
App ID 18936
307 Da
13. Leu-Leu-Leu
App ID 18945
14. L-Glutathione
Sample: 1. Thyroglobulin 669 kDa

2. lgG 156 kDa
3. BSA 66 kDa
4. Ovalbumin 45 kDa
5. Myoglobin 16.9 kDa
6. Uridine 244 Da
BioSep-SEC-s3000
300 x 7.8mm
00H-2146-K0
Flow Rate: 1mL/min
1
Detection: UV @ 280nm
BioSep-SEC-S | proteins/peptides | BIOSEPARATIONS
BioSep-SEC-s2000
300 x 7.8mm
00H-2145-K0
45% Acetonitrile, 0.1% TFA
1mL/min
Ambient
UV @ 214nm

0
0
Phenomenex
10
15
min
10
12
14
min
293
BioSep -SEC-S
18906
Human
IgG2k Aggregates
100
Flow Rate:
Temperature:
Detection:
Sample:
80
40
20
1
5
2
10
12
14 min
2
10
14
18965
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:
Murine IgG1k Aggregates
BioSep-SEC-s3000
300 x 7.8mm
00H-2146-K0
1mL/min
Ambient
UV @ 214nm
Human Serum
Human Serum Albumin (HSA)
mAu
25
Column:
Dimensions:
Part No.:
Mobile Phase:
20
Flow Rate:
Temperature:
Detection:
Sample:
15
Human Serum Proteins
10
BioSep-SEC-s3000
300 x 7.8mm
00H-2146-K0
50mM Sodium Phosphate
pH 6.8,
1mL/min
Ambient
UV @ 220nm
1. HMW aggregates
2. IgG1 kappa dimer 1
3. IgG1 kappa dimer 2
4. IgG Monomer
5. Low MW impurity
6. Void Volume Peak
App ID 18937
Human Serum and HSA
Human Serum Albumin
3
5
2
0
0
10
12
14
min
Medium MW Protein Mixture

Column:
Dimensions:
Part No.:
Mobile Phase:
18931
Flow Rate:
Temperature:
Detection:
Sample:
10
12
Column:
Dimensions:
Part No.:
Mobile Phase:
18904 Flow Rate:
Temperature:
mAU
Detection:
Sample:
100
BioSep-SEC-s2000
300 x 7.8mm
00H-2145-K0
100mM Sodium Phosphate pH 6.8
1mL/min
Ambient
UV @ 220nm
1. 2 PEG Modified Complex
2. PEGylated b-Lactoglobulin
3. b-Lactoglobulin
4. PEG Reagent
80
2
60
10
12
14
min
3
20
App ID 18904
App ID 18931
40
min
PEGylated b-Lactoglobulin A (N-Terminal PEG 20 kDa)
BioSep-SEC-s3000
300 x 7.8mm
00H-2146-K0
1mL/min
7
Ambient
5
UV @ 214nm
4
1. HMW impurity
2. Thyroglobulin 670 kDa
3
3. IgA 300 kDa
6
5. IgG 150 kDa
6. Transferrin 80 kDa
7. Ovalbumin 45 kDa
8
8. b-Lactoglobulin A 35 kDa
9. Uridine 224 Da
1
294
min
18937
App ID 18965
BIOSEPARATIONS | proteins/peptides | BioSep-SEC-S
60
Column: BioSep-SEC-s2000
Part No.: 00H-2145-E0
Mobile Phase: mAU
300mM
Sodium Chloride
25
20 @ 220nm
Detection: UV
Sample: Recombinant Human Erythropoietin
1. HMW impurity
15
2. EPO dimer
3. EPO monomer
4.
10 LMW impurity
App ID 19137
120
BioSep-SEC-s3000
300 x 7.8mm
00H-2146-K0
100mM Sodium Phosphate
pH 6.8
1mL/min
Ambient
UV @ 220nm
1. IgG aggregate peak
2. IgG trimer peak
3. IgG dimer peak #1
4. IgG dimer peak #2
5. IgG Monomer
6. IgG low MW fragment
App ID 18906
140
Column:
Dimensions:
Part No.:
Mobile Phase:
Recombinant Human Erythropoietin (HuEPO)
Overnight
60 min
0 min
Intact
2
10
12
14 min
Phenomenex
BioSep -SEC-S
If BioSep analytical columns do not

separation as any other GFC column of
similar porosity, type and dimensions,
Global support and availability in over 65 countries

3 batches available for validation
Large inventory for immediate shipment
Technical Data and Specifications
Cleaning Procedure
Storage
Column Protection
BioSep SEC-s2000
BioSep SEC-s3000
BioSep SEC-s4000
Silica
5
145
Silica
5
290
Silica
5
500
1,000 - 300,000
200 - 75,000
500 - 100,000
2.5 - 7.5
1,500
800
30,000
5,000 - 700,000
5,000 - 100,000
1,000 - 150,000
2.5 - 7.5
1,500
800
30,000
15,000 - 1,500,000
15,000 - 500,000
5,000 - 700,000
2.5 - 7.5
1,500
700
25,000
This is a function of pressure. Columns can withstand up to 1,500 psi, but avoid sudden pressure changes.
Standard: 316 stainless steel column with stainless steel frits. Titanium frits available.
50 C
1M
0.5% SDS, 6 M Guanidine HCI, or 8 M urea
After exposure to denaturants, wash with water overnight.
Up to 100% CH3CN. Start with 100% H2O, linear gradient to 100% CH3CN over 50 min.
Up to 90% CH3CN, 10% DMSO or 500mM b-mercaptoethanol.
General protein removal: wash with 30mL of 0.1 M NaH2P04, pH 3.0.
Hydrophobic protein removal: use acetonitrile gradient.
Strongly adsorbed proteins: wash with 30mL of 0.5% SDS or 6 M Guanidine thiocyanate or 10% DMSO.
Overnight storage: run mobile phase at 0.2mL/minute. Prolonged storage: use 0.05% NaN3 in H2O or 10% methanol in H20.
Use of a SecurityGuard is recommended to prolong column lifetime.
Cross Reference Chart

Phenomenex
BioSep Phases
SEC-s2000
SEC-s3000
SEC-s4000
Bio-Rad
Bio-Sil SEC 125
Waters
BioSuite
BioSuite 125
Zorbax
GF-250
PROTEIN KW-803
Sepax
SRT-100*
SRT-150
SRT-300
Bio-Sil SEC 250
BioSuite 250
GF-450
PROTEIN KW-804
SRT -500**
Bio-Sil SEC 400
BioSuite 450**
Shodex
PROTEIN KW-802.5
TSKgel
G2000SW
G2000SWXL
G3000SW
G3000SWXL
G4000SW
G4000SWXL
Stainless Steel Columns (mm):

Phases
Narrow Bore
300 x 4.6
300 x 7.8
600 x 7.8
Preparative
300 x 21.2
BioSep-SEC-s2000
BioSep-SEC-s3000
BioSep-SEC-s4000
00H-2145-E0
00H-2146-E0
00H-2147-E0
00H-2145-K0
00H-2146-K0
00H-2147-K0
00K-2145-K0
00K-2146-K0
00K-2147-K0
00H-2145-P0
00H-2146-P0
00H-2147-P0
Stainless Steel Guard Columns (mm)

Phases
Narrow Bore
30 x 4.6
03A-2145-E0
03A-2146-E0
03A-2147-E0
Analytical
Express
35 x 7.8
03Q-2145-K0
03Q-2146-K0
03Q-2147-K0
For Column Heaters, see p. 357.
Phenomenex
** Only up to 1,500,000 MW
* Only above 1,000 MW
*SecurityGuard Analytical cartridges require holder, Part No.: KJ0-4282

** PREP SecurityGuard Cartridges require holder, Part No.: AJ0-8223
BioSep-SEC-s2000
BioSep-SEC-s3000
BioSep-SEC-s4000
BioSep-SEC-S | proteins/peptides | BIOSEPARATIONS
Resin Type
Particle Size (m)
Pore Size ()
Exclusion Range in Daltons for Proteins:
Native
0.5 % SDS
6 M GnHCI
pH Range
Maximum Backpressure (psi)
Typical Backpressure (psi)
Efficiency
(minimum number theoretical plates 300 x 7.8mm)
Maximum Flow Rate
Column Hardware
Maximum Temp.
Maximum Salt Conc.
Denaturants
Regeneration
Max. Organic Modifier
Analytical
75 x 7.8
03C-2145-K0
03C-2146-K0
03C-2147-K0

4 x 3.0*
15 x 21.2**
/10pk
ea
AJ0-4487
AJ0-8588
AJ0-4488
AJ0-8589
AJ0-4489
AJ0-8590
for ID: 4.6-7.8mm for ID: 21.2mm
Aqueous SEC 1 Column Check Standard
(for BioSep-SEC-S and other protein SEC columns)
Part No.: AL0-3042
Price:
Unit quantity: Dry; reconstituted to 2mL
Contains: Bovine thyroglobulin; Human gamma globulin
(contains IgA and IgG); Ovalbumin; Myoglobin;
Uridine (reconstitute with 1mL of 100mM
Sodium phosphate pH 6.8)
Diluent: 100mM Sodium phosphate pH 6.8
Storage: Add 0.1% NaN3 to the solution and refrigerate
Test Conditions
Mobile phase:
Flow rate:
Injection volume:
Detection:
100mM Sodium phosphate, pH 6.8

1.0mL/min for a 300 x 7.8mm column
10L
UV @ 280nm
295
Jupiter
If Jupiter analytical columns do not

separation as compared to a column
of similar phase, particle size and
dimension, return the column with
FULL REFUND.
RP-HPLC for Protein/Peptide

Analysis and Purification
The Jupiter HPLC column portfolio, including Jupiter 300 and Jupiter
Proteo, offers optimized reversed phase solutions for protein and
peptide characterization and purification. With these columns, one
can identify, purify, and analyze almost any protein.
Jupiter 300 300 columns designed to analyze and purify

intact proteins
It is tough to compete with Jupiter standards. Each column has

consistent specifications and thus consistent performance.
pH 1.5-10 stability gives robust, method development
opportunities
For separation of intact proteins > 10,000 MW

Available with C18, C5, and C4 bonded phases
BIOSEPARATIONS | proteins/peptides | jupiter
Engineered for Robustness,

Reproducibility, and Quality
Over 25 individual quality control tests performed on every

batch of Jupiter material
1.5 10 pH stability for method ruggedness and easy

protein removal
Every column reproducibility aspect is specified, tested, and

reported in Materials Validation Document (MVD)
Direct scale up to preparative and bulk materials
Jupiter Proteo 90 columns engineered for increased

peak capacity and resolution of peptide maps as well as peptide
separations
For separation of intact proteins and peptides < 10,000 MW
Available with novel C12 bonded phase for excellent
selectivity
pH 1.5 10 Stability
A wide pH range means opportunity for method development, in
addition to longer column life. Jupiter columns are stable for over
2500 hours at pH extremes. Jupiter 300 and Jupiter Proteo provide
excellent separations using various MS compatible buffers and
provide good resolution down to 0.01% TFA.
Identify post-translational modifications

Capillary columns available for increased sensitivity
Stability of Jupiter 300 C18 at pH 1.5 and 10
Packing
Material
C4
C5
C18
Proteo
Particle
Shape/Size
(m)
Pore
Size
()
Surface
Area
(m2/g)
Carbon
Load
%
Calculated Bonded
Phase Coverage
(mole/m2)
End
Capping
Spher. 5, 10, 15
Spher. 5, 10, 15
Spher. 3, 5, 10, 15
Spher. 4, 10
300
300
300
90
170
170
170
475
5.0
5.5
13.3
15.0
6.30
5.30
5.50
Yes
Yes
Yes
Yes
Dependable Solutions for Protein/Peptide

Researchers
Hours of Exposure
Test Conditions: Column flushed
in 20 mM Na2HPO4 (pH 10.0)
in Water/Acetonitrile (50:50)
Hours of Exposure
Test Conditions: Column flushed
in 0.1 % TFA (pH 1.5) in
Water/Acetonitrile (50:50)
Jupiter HPLC columns are used throughout the life science industry in a variety of departments. Phenomenex offers support
and solutions in all areas of protein research and manufacturing,
especially in characterization, purification, and proteomics/biomarker discovery.
Type of Work
Protein Characterization
Protein/ Peptide Purification

Proteomics/ Biomarker
Discovery
Key Applications
Identify post-translational modifications
Analyze intact antibodies
Study PEGylated proteins
Separate target compound from impurities
Purify antibodies
Separate protein components from one another
Easy, direct scale-up to preparative and process scales
Perform peptide mapping for differential proteomics
Identify low level proteins utilizing capillary columns
Excellent resin for 2nd dimension of 2D-HPLC
(see pp. 298-299 for more information)
296
Phenomenex
Jupiter
Quality Proven
A Materials Validation Document (MVD) accompanies every Jupiter
column. Each certificate documents the rigorous testing procedures
performed on each batch of Jupiter material to ensure column-tocolumn and batch-to-batch reproducibility.
Silica physical
tests and
specifications
Sem analysis
Scanning Electron
Microscopy (SEM)
photos show surface
smoothness and
particle consistency as
well as a visual representation of particle
size distribution.
Capillary-LC columns packed with Jupiter 300 and Jupiter Proteo materials provide the same characteristics as their respective
analytical-sized columns, but with added sensitivity for microliter
sample volumes. The high purity silica combined with proprietary
bonding techniques produce dense bonded phase coverage to
shield against silanol interaction. With few secondary chromatographic effects, Jupiter capillary-LC columns yield terrific peak
shape and are compatible with reduced TFA concentration and
other mass spec compatible mobile phase modifiers.
High sensitivity for low volume and mass limited samples
Consistent performance under LC/MS compatible and
universal conditions
Diagnostic
chromatography
tests
High efficiency 0.30 and 0.50 mm ID glass-lined columns
Monitoring chromatographic specifications

for silanol activity,
hydrogen bonding capacity, hydrophobicity
and peptide standards.
-Chymotrypsin Tryptic Digest on Jupiter Proteo

Column:
Dimensions:
Part No.:
Mobile Phase:
pH stability
Every batch goes

through 1.5 and
10.0 pH testing
before release, the
results of which are
reported on each
MVD.
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:
Jupiter 4 m Proteo 90
150 x 0.50 mm
00F-4396-AF
A: 0.01 % TFA in Water
A/B (95:5) for 5 min then
A/B (55:45) in 45 min
Switch to A/B (95:5) in 1 sec then hold for 10 min
15 L/min
40 C
UV @ 210 nm
- Chymotrypsin tryptic digest
App ID 14336
Reproducibility Assured
Batch-to-batch and column-to-column is critical to HPLC column
performance. Through great advances in silica, bonding, and material characterization technology, Jupiter columns set a new benchmark in reproducibility.
Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
10
20
30
40 min
Batch 6
Batch 5
Batch 4
Batch 3
Batch 2
Batch 1
App ID 16658
Flow Rate:
Detection:
Sample:
Jupiter 5 m C18 300

250 x 4.6 mm
00G-4053-E0
A/B (75:25) to A/B (45:55)
in 15 min
1.0 mL/min
UV @ 220 nm
1. Yeast Cytochrome c
2. Equine Cytochrome c
3. Bovine Cytochrome c
jupiter | proteins/peptides | BIOSEPARATIONS
Pore size, particle

size and distribution,
metal content, surface
area, carbon load
and surface coverage
specifications and
results are all reported.
Capillary Formats for

Increased Sensitivity
Phenomenex
297
Jupiter
Jupiter 300 has proven its performance to biochromatographers

worldwide as a leading 300 column in the market.
300 column for analysis and purification of proteins
> 10,000 MW
Highly efficient columns with long lifetime due to
super-smooth, high-mechanical-strength 300 silica
Large Proteins on Jupiter 300 C4

Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Temperature:
Detection:
Inj. Volume:
Sample:
Excellent column for LC/MS due to low phase bleed and

exceptional performance at low buffer concentrations
Jupiter 300 5 m C4 300

150 x 4.6 mm
00F-4167-E0
1 mL/min
22 C
UV @ 280 nm
25 L
1. Bovine Serum Albumin
2. Glutamic Dehydrogenase
3. -Galactosidase
4. Ovalbumin
3
1
App ID 14761
Jupiter 300 for Intact Protein

Separation and Purification
Jupiter 300 C4 This low hydrophobicity phase is less likely to cause

irreversible adsorption of sticky proteins and allows for the use
of shallow gradients along with lower concentrations of organic
solvent.
For proteins >10,000 Da
For highly hydrophobic proteins
Jupiter 300 C5 This bonded phase imparts greater pH stability compared to the traditional C4 phase. One can expect longer column
lifetimes and more stable, reproducible retention times because of
the bonded phases increased stability to hydrolysis.
Separation on Jupiter 300 C18

Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Sample:

For highly hydrophobic proteins
More retentive than C4, offering slightly different selectivity
Jupiter 300 5 m C18 300

150 x 2.0 mm
00F-4053-B0
A: 0.1 %TFA/ 95 % Water / 5 % Acetonitrile
B: 0.085 % TFA/ 95 % Acetonitrile/ 5 % Water
0.2 mL/min
mAU
UV @ 220 nm
800
1. Aprotinin
2. Ribonuclease
600
3. Lysozyme
1
4. Lactalbumin
400
5. Leptin
App ID 14937
Selecting the Appropriate 300 Phase
200
0
Jupiter 300 C18 Excellent for polar as well as non-polar proteins.

Its the most retentive of Jupiter 300 phases, allowing one to separate proteins with slight differences in hydrophobicity.
For hydrophilic proteins
Most retentive phase
10
12
14
16
18 min
Sharp Peaks and High Yields

Ultra-pure silica and dense bonded phase coverage provide sharp
peaks for your sample by decreasing the number of non-specific
interactions. Sharpened peak symmetry allows for easier quantitation, improved resolution, and separation of complex mixtures. High
yields result in more material for downstream analysis, decreased
number of purification runs, and lower production costs.
Sharp Peaks for Easy Quantitation
Gradient:
Flow Rate:
Detection:
Sample:
Jupiter 300 5 m C4 300

50 x 4.6 mm
00B-4167-E0
A: 0.1% TFA in Water
B: 0.1% TFA in Acetonitrile
A/B (100:0) to A/B (80:20) in 1 min
(20 % B/ min), then to A/B (65:35) in
1.5 min (10 % B/ min), then to A/B
(53.5:46.5) in 1.5 min (7.67 % B/ min),
hold for 2 min (constant B)
1 mL/min
UV @ 220 nm
1. Alkaline Phosphatase
2. Cyanocobalamin
3. RNase
4. Insulin
5. Transferrin
6. Trypsin Inhibitor
App ID 5378
Column:
Dimensions:
Part No.:
Mobile Phase:
2.5
298
7.5
min
Phenomenex
Jupiter
Protein/Peptide Purification
Achieving adequate separation of proteins is one of the biggest
challenges in chromatographic purification. Reversed phase HPLC
offers a very high speed, high efficiency, and high resolution solution for protein purification. In addition, scale-up from analytical to
prep to process is easy and convenient with the various particle
sizes and column dimensions available.
Purify proteins from their degradation products
Successfully purify target proteins and peptide therapeutics
Easily scale up from lab-scale to process-scale purification

Column: Jupiter 4 m Proteo 90
Part No.: 00G-4396-E0
B: 0.085 % TFA in 95:5 Acetonitrile / Water
Gradient: A/B (80:20) to A/B (20:80) in 15 minutes
Detection: UV @ 220 nm (ambient)
Sample: Human Insulin
Jupiter uses identical bonding and base silica technology in both

analytical and preparative materials. Accordingly, Jupiter 300 material used in analytical separation is available in a 10 m and 15 m
(Jupiter Proteo is available in 10 m) version so you can easily scale
up with minimal changes to the separation.
Large loading capacity for higher sample recovery
Easy material cleaning and regeneration
mAU
Resistance to silica sheering and fine formation at high

packing pressures and flow rates
App ID 14914
400
15 min @ 60 C
200
0
2
60 min @ 60 C
200
Virgin Bulk Media
After Packing
App ID 14917
App ID 14915
200
10 min
10 min
HPLC separations on Jupiter Proteo at different time points of insulin incubated under basic conditions
at 60 C. There was an increase of numerous degradation products with extended exposure at elevated
temperature that Jupiter Proteo was able to separate.
Separation of Insulin Genetic Variants

Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Sample:
Jupiter 300 5 m C18 300

250 x 4.6 mm
00G-4053-E0
A/B (70:30) to A/B (68:32) in 20 min
1.0 mL/min
UV @ 210 nm
1. Bovine Insulin
2. Human Insulin
3. Porcine Insulin
mAU
Alltech Associates Vydac

5 m C18 300
mAU
App ID 5372
0
2.5
5.0
7.5
10.0
12.5
15.0
17.5 min
Bovine, human, and porcine insulin were purified away from each other due to the strong
resolving power of Jupiter 300 C18.
400
200
Phenomenex
Separate Key
Components 2
400
600
200

Column: Jupiter 300 5 m C18 300
Vydac 5 m C18 300 (238EV52-Everest)
Mobile Phase: A: 0.1 %TFA/ 95 % Water/ 5 % Acetonitrile
B: 0.085 % TFA/ 95 % Acetonitrile/ 5 % Water
Gradient: A/B (80:20) to A/B (15:85) in 15 minutes
Sample: 1. Aprotinin
4. Fibrinogen
2. Ribonuclease
5. Leptin
3. Acid Glycoprotein
Jupiter 300 5 m C18 300
800
400
Purify Key Proteins from One Another
App ID 14925
App ID 14924
mAU
Jupiter
10 m C18 300
mAU
30 min @ 60 C
1000
Jupiter
10 m C18 300
10 min
mAU
jupiter | proteins/peptides | BIOSEPARATIONS
Easy Scale-Up with Prep Columns

and Bulk Material
Insulin Purified from its Degradants
5
200
0
0
10
12
14
16 min
10
12
14
16 min
Jupiter has excellent resolving power as seen in the above example. Resolution is extremely important,
especially when impurities can differ by only a few amino acids.
Vydac is a registered trademark of Alltech Associates, Inc. Phenomenex is in no way affiliated with Alltech
Associates. Comparative separations may not be representative of all applications.
299
If Jupiter analytical columns do not provide you with at least

an equivalent separation as compared to a column of similar
phase, particle size and dimension, return the column with
comparative data within 45 days for a FULL REFUND.
Jupiter
4 m & 5 m Capillary Columns (mm)
Phases
50 x 0.30
150 x 0.30
50 x 0.50
150 x 0.50
250 x 0.50
00G-4167-AC
00G-4053-AC
00G-4396-AC
00B-4167-AF
00B-4053-AF
00B-4396-AF
00F-4167-AF
00F-4053-AF
00F-4396-AF
00G-4053-AF
00G-4396-AF
3 m, 4 m & 5 m Microbore and Minibore Columns (mm)

Phases
50 x 1.0
150 x 1.0
250 x 1.0
50 x 2.0
150 x 2.0
00B-4167-B0
00B-4052-B0
00B-4053-B0
00B-4396-B0
00F-4167-B0
00F-4052-B0
00F-4053-B0
00F-4396-B0
00B-4263-B0
00F-4263-B0
5 m C4 300
5 m C18 300
4 m Proteo 90
5 m C4 300
5 m C5 300
5 m C18 300
4 m Proteo 90
Capillary Guard Columns (mm)

20 x 0.30
20 x 0.50
250 x 0.30
3 m C18 300
00B-4167-AC
00B-4053-AC
00B-4396-AC
00B-4167-A0
00B-4052-A0
00B-4053-A0
00B-4396-A0
00F-4167-AC
00F-4053-AC
00F-4396-AC
00F-4167-A0
00F-4052-A0
00F-4053-A0
00F-4396-A0
00G-4167-A0
00G-4052-A0
00G-4053-A0
00G-4396-A0
03M-4167-AF
03M-4053-AF

250 x 2.0
4 x 2.0*
/10pk
00G-4167-B0
AJ0-4329
00G-4052-B0
AJ0-4326
00G-4053-B0
AJ0-4320
00G-4396-B0
AJO-6073
/10pk
AJ0-4320
for ID: 2.0-3.0 mm
3 m, 4 m & 5 m Analytical, Semi-Prep, and Preparative Columns (mm)

Phases
50 x 4.6
150 x 4.6
250 x 4.6
250 x 10
5 m C4 300
5 m C5 300
5 m C18 300
4 m Proteo 90
3 m C18 300
00B-4167-E0
00B-4052-E0
00B-4053-E0
00B-4396-E0
00F-4167-E0
00F-4052-E0
00F-4053-E0
00F-4396-E0
00G-4167-E0
00G-4052-E0
00G-4053-E0
00G-4396-E0
00F-4263-E0
00G-4263-E0
00G-4167-N0
00G-4052-N0
00G-4053-N0
00G-4396-N0
250 x 21.2
00G-4167-P0
00G-4052-P0
00G-4053-P0
00G-4396-P0

4 x 3.0*
10 x 10
15 x 21.2**
/10pk
/3pk
/ea
AJ0-4330
AJ0-7225
AJ0-7231
AJ0-4327
AJ0-7371
AJ0-4321
AJ0-7224
AJ0-7230
AJ0-6074
AJ0-7275
AJ0-7842
/10pk
AJ0-4321
for ID: 3.2-8.0 mm
10 m Analytical, Semi-Prep, and Preparative Columns (mm)

Phases
250 x 4.6
250 x 10
250 x 21.2
C4 300
C5 300
C18 300
Proteo 90
00G-4168-E0
00G-4054-E0
00G-4055-E0
00G-4397-E0
00G-4168-N0 00G-4168-P0
00G-4054-N0 00G-4054-P0
00G-4055-N0 00G-4055-P0
00G-4397-N0
250 x 30
aufInquire
Anfrage
00G-4168-U0
00G-4055-U0
250 x 50
aufInquire
Anfrage
00G-4168-V0
00G-4054-V0
00G-4055-V0
4 x 3.0*
/10pk
AJ0-4330
AJ0-4327
AJ0-4321
AJ0-6074
C4 300
C5 300
C18 300
00G-4169-E0
00G-4056-E0
00G-4057-E0
00G-4169-N0 00G-4169-P0
00G-4056-N0 00G-4056-P0
00G-4057-N0 00G-4057-P0
250 x 30
aufInquire
Anfrage
00G-4169-U0
00G-4057-U0
250 x 50
aufInquire
Anfrage
00G-4169-V0
00G-4056-V0
00G-4057-V0
4 x 3.0*
/10pk
AJ0-4330
AJ0-4327
AJ0-4321
9-16 mm
18-29 mm
18-29 mm

10 x 10
15 x 21.2**
/3pk
/ea
AJ0-7225
AJ0-7231
AJ0-7371
AJ0-7224
AJ0-7230
for ID: 3.2-8.0 mm
For Jupiter Proteo Axia Packed

Preparative columns, see pp. 342-343
9-16 mm

10 x 10
15 x 21.2**
/3pk
/ea
AJ0-7225
AJ0-7231
AJ0-7371
AJ0-7224
AJ0-7230
AJ0-7275
AJ0-7842
for ID: 3.2-8.0 mm
15 m Analytical, Semi-Prep, and Preparative Columns (mm)

Phases
250 x 4.6
250 x 10
250 x 21.2
300
03M-4167-AC
03M-4053-AC
03M-4396-AC
Bulk Material
10 m Bulk Packings
Phase
100 g
aufInquire
Anfrage
C4 300
04G-4168
C5 300
04G-4054
C18 300
04G-4055
Proteo 90
04G-4397
1 kg
aufInquire
Anfrage
04K-4168
04K-4054
04K-4055
04K-4397
5 kg
aufInquire
Anfrage
04L-4168
04L-4054
04L-4055
04L-4397
10 kg
aufInquire
Anfrage
04M-4168
04M-4054
04M-4055
04M-4397
50 kg
aufInquire
Anfrage
04N-4168
04N-4054
04N-4055
04N-4397
100 kg
aufInquire
Anfrage
04P-4168
04P-4054
04P-4055
04P-4397
15 m Bulk Packings
Phases
100 g
aufInquire
Anfrage
C4 300
04G-4169
C5 300
04G-4056
C18 300
04G-4057
1 kg
aufInquire
Anfrage
04K-4169
04K-4056
04K-4057
5 kg
aufInquire
Anfrage
04L-4169
04L-4056
04L-4057
10 kg
aufInquire
Anfrage
04M-4169
04M-4056
04M-4057
50 kg
aufInquire
Anfrage
04N-4169
04N-4056
04N-4057
100 kg
aufInquire
Anfrage
04P-4169
04P-4056
04P-4057
9-16 mm
18-29 mm
15 x 30.0
/ea
AJ0-8314
AJ0-8313
AJ0-8304
30-49 mm
15 x 30.0
/ea
AJ0-8314
AJ0-8313
30-49 mm

For Fused Silica Capillary Adapter,

see p. 360
For Column Chiller/Heater System

(8-70 C), see p. 357
Effectively desalt acidic, basic, and neutral

peptides with Strata-X. See p. 39 for more
information.
For SecurityGuard Cartridge Holders

and Cartridges, see p. 235
Phenomenex
Clarity BioSolutions for Synthetic DNA/RNA
U.S. Patent No. 7, 119, 145
Optimized Oligo Purification

and Analysis
RPC, HPLC, prep LC, desalting, and extraction solutions
DNA, RNA/RNAi, longmers, dye-labeled, and modified
oligonucleotides
High efficiency LC/MS protocols for characterization/QC
Personalized technical support and customer service
Clarity Products
Clarity QSP Cartridges

Clarity Oligo-RP
HPLC Columns
Clarity Oligo-WAX
HPLC Columns
Clarity RP-Desalting Tubes
Clarity Oligo-MS
HPLC Columns
Clarity OTX
Extraction Plates
Particle
Support
Bonded
Phase
Particle
Shape/Size
(m)
Pore
Size
()
Surface
Area
(m2/g)
Polymer (PSDVB)
TWIN
(silica, organic composite)
Silica
Hydrophilic polymer coating

C18
35
3, 5, 10
500
110
300
375
Crosslinked polyamine (WAX)
10
360
160
Silica
Core-Shell
C18
C18
55
1.7, 2.6
140
100
Polymer (surface
modified PSDVB)
Mixed-mode anion exchanger
33
85
300
200*
(*effective)
800
clarity | synthetic DNA/RNA | BIOSEPARATIONS
Each product in the Clarity BioSolutions portfolio has been

designed to efficiently and effectively purify or characterize synthetic
oligonucleotides used in biological research, therapeutic development
and biochemical manufacturing. Purification solutions include reversed
phase HPLC (RP-HPLC), ion-exchange LC (IEX-LC), reversed phase
cartridge (RPC), and desalting, while characterization solutions include
high efficiency reversed phase (RP-LC/MS) columns.
Clarity BioSolutions Product Selection

Purification
Clarity QSP
Primary Use
Purities
Recoveries
Synthesis Scale
Load
Oligo Types
Clarity Oligo-RP
Clarity Oligo-WAX
High-throughput, trityl-on RP-HPLC purification of failEconomical, high loading

RPC purification
ure from target sequences IEX-LC prep-scale purification
>90 %
>90 %
>90 %
~90 %
~70 %
>90 %
Up to 50 mole
Up to 50 mole
Up to 50 mole
Clarity RP-Desalting
Quick removal of salt

& excess reagent
~70 %
~70 %
Up to 1 mole
DNA, RNA/RNAi, Thioates, Dye-labeled, Modified
Characterization / Analysis
Primary Use
Oligo Length
Recommended
Mobile Phase
Phenomenex
Clarity Oligo-RP
Clarity Oligo-MS
Clarity OTX
RP-LC/MS analysis with

optimized selectivity and
sensitivity
60 mer
TEA / HFIP
Rapid, high efficiency RPLC/MS analysis for QC and

characterization
60 mer
tea/hfip/MeOH
Extraction of oligo therapeutics from biological samples

for LC/MS bioanalysis
40 mer
n/a
301
U.S. Patent No. 7, 119, 145
Clarity OTX Extraction Kits

Rapid Cleanup of Oligo Therapeutics
from Biological Samples
Designed for Throughput

In just 4 steps and 15 minutes, scientists can
extract therapeutic oligos and their metabolites
from biological samples. This is accomplished by
eliminating the need for liquid-liquid extraction and
providing a 96-well plate format which is
amenable to parallel processing.
> 80 % typical extraction recoveries

No liquid-liquid extraction (LLE) required
MeOH
Suitable for a majority of therapeutic oligos, tissues,

and fluids
STEP 1
Effective Recovery
The Clarity OTX extraction solution was designed to effectively
isolate a wide range of therapeutic oligonucleotides from fluids
and tissues. It utilizes a mixed-mode solid phase extraction
sorbent in conjunction with carefully formulated buffers to
consistently deliver greater than 80 % recoveries.
Equil.
Buffer
Optimized for LC/MS bioanalysis
BIOSEPARATIONS | synthetic DNA/RNA | clarity OTX
2010 R&D 100

Award Recipient
Preparation of SPE sorbent to

selectively retain the oligo of
interest and its metabolites.
Sample Preparation:
Add an equal volume of Lysis-Loading buffer to biological fluid matrix
STEP 2
Vortex briefly
Extraction Protocol
Load Sample
Salts, sugars, large proteins and

genomic DNA flow through the
cartridge. The oligo of interest,
proteins, and lipids bind to the
sorbent via a mixed-mode, weak
anionic interaction.
Condition: 1 mL Methanol (Vacuum ~2 Hg)

Equilibrate: 1 mL Equilibration buffer (Vacuum ~3 Hg)
Load sample: 0.4 mL - 3 mL volume (Vacuum ~3 Hg)
Vacuum: ~10 Hg for ~10 seconds to completely evacuate solution through cartridge
Wash: 6 mL Wash buffer (2 mL x 3) (Vacuum 3-4 Hg)
Vacuum: 10-15 Hg for ~1 minute
Elute: 1 mL Elution buffer (Vacuum ~3 Hg)
LC/MS Prep: Dry down or lyophilize and reconstitute in 100 L water or aqueous buffer
18868
UV Recovery Data
Standard 19mer phosphorothioate (10 g)

Area: 2315
40
App ID 18868
Plasma extracted 19mer phosphorothioate (10 g)

Area: 2078
Recovery: 90 %
80
The Wash Buffer is formulated

to strip off lipids and remaining
proteins from the sorbent,
while not disturbing the oligo
therapeutics and its metabolites.
Wash
Buffer
STEP 3
mAU
0
0
10
15
20
min
MS Recovery Data
App ID 18869
The addition of the Elution

Buffer releases the target oligo
therapeutic and its metabolites.
The elution volume can be
dried down or lyophilized and
reconstituted prior to LC/MS
anlaysis.
The above illustrates the recovery of a 19mer thioate from 200 L of human plasma. The
UV data shows that 90 % recovery is achieved with the Clarity OTX extraction protocol.
The MS data further demonstrates that plasma contaminants are effectively removed and
complete isolation and recovery of the target is achieved.
302
Elution
Buffer
STEP 4
Oligo & metabolites

Salts
Sugars
Genomic DNA
Lipids
Proteins
Request a FREE copy of the Clarity OTX Users Guide for more
detailed information on the extraction protocol.
Phenomenex
U.S. Patent No. 7, 119, 145
Clarity OTX (cont'd)

Flexible Formats
To test proof of concept or for low
sample volumes, Clarity OTX is
available as a starter kit, which
includes 50 solid phase extraction
cartridges and all the buffers (lysisloading, equilibration, wash, and
elution) required for the extraction
protocol.
Detect Low Dosage Levels

Due to the typical 85% and greater recoveries of the parent oligonucleotide therapeutic and its metabolites and the elimination
of interfering compounds, detection in low sensitivity ranges is
possible when using a sensitive MS.
19mer
Oligo
mAU
100
Internal
Std.
10 g oligo in 1 g liver tissue
50
2
For labs that must process large volumes

of biological samples, 96-well plates, 1 L
quantities of lysis-loading buffer, and the
formulations for the other three buffers
are available.
min
min
min
100 g oligo in 1 g liver tissue
100
2
mAU
100 g oligo and 100 g internal

standard
100
50
2
App ID 18872
50
UV chromatograms of oligonucleotide extracted from liver tissue using Clarity OTX.

The 19mer extracted phosphorothioate oligonucleotide was spiked with 10 g of a
oligonucleotide internal standard before analysis. The top two chromatograms represent
different levels of the incubated P-S oligo. The bottom chromatogram is an external
standard of equal amounts of the 19mer oligo and internal standard. Note the high recovery
of the oligonucleotide and low level of plasma contaminants from the incubated samples.
Eliminate MS Interfering Contaminants

The Clarity OTX extraction protocol effectively removes cell debris
such as proteins, genomic DNA, and lipids which significantly mask
the oligo therapeutics of interest. By removing these contaminants,
MS noise is considerably reduced.
Excellent Linearity
Significant effort was made to develop an extraction solution that
would provide good linearity and reliable quantitative results.
Liver Tissue Linearity Curve
y = 171677x + 2E+06
= 0.998
1.60E+07
Peak Area
1.20E+07
Clarity OTX
Part No.
Description
KS0-8494
Clarity OTX
Starter Kit
Includes:
100 mg/3 mL cartridges (x50)
Lysis-loading buffer (60 mL)
Equilibration buffer (250 mL)
Wash buffer (350 mL)
Elution buffer (60 mL)
8E-S103-EGA Clarity OTX Well Plate 100 mg/ well
8B-S103-EBJ Clarity OTX Cartridge 100 mg/3 mL
AL0-8498
Clarity OTX
1L
Lysis-Loading Buffer
1L
AL0-8579
Clarity OTX
Lysis-Loading Buffer
V2.0
Unit
ea
Price
clarity otx | synthetic DNA/RNA | BIOSEPARATIONS
mAU
1/box
50/box
ea
ea
8.00E+06
4.00E+06
0.00E+00
20
40
60
g/g Liver
80
100
120
From low to high concentrations of ng/mL, excellent linearity is achieved on the MS by

extracting oligo therapeutics and their metabolites using the Clarity OTX methodology.
Linearity for a 19mer P-S oligonucleotide in 1 g of liver tissue, based on MS peak area,
was evaluated at four different oligo concentrations in liver tissue from 100 g to 10 g.
High recovery and good linearity is seen across physiological relevant concentrations for
this initial study.
Phenomenex
303
U.S. Patent No. 7, 119, 145
Clarity QSP Cartridges and 96-Well Plates

High-throughput, RPC Purification
> 90 % typical purities & recoveries for RNA & DNA
For oligos 10 100 mer
Simple 3-step process for trityl-on oligos
Amenable to parallel processing
BIOSEPARATIONS | synthetic DNA/RNA | clarity qsp
Cost-effective solution for high purity

The Quick, Simple, Pure (QSP) Protocol
Following the easy, step-by-step QSP protocol anyone can
deliver high purity RNA and DNA. The process includes brief
sample preparation followed by 3 simple steps to isolate the
oligo of interest from impurities and failure sequences. The
QSP sorbent and loading buffers have been engineered to
work synergistically with crude synthetic mixtures to produce
greater than 90 % recoveries and purities in less than 20
minutes.
Dual-Component System
Two components, loading buffer and SPE
cartridge or 96-well plate, are required for
Clarity QSP purification. Various loading
2008 R&D 100
Award Recipient
buffers have been formulated specifically for
DNA and RNA chemistries so that one-step
loading in synthetic cocktails is permissible and no ion-pairing
reagents are required. Multiple SPE formats are available to suit
a wide range of synthesis scales and automation requirements.
96-well plates are of a standard footprint and should fit most
commercial vacuum manifolds and liquid handling robots.
Loading Buffers
DNA: for all DNA and RNA-TOM chemistries
RNA-TBDMS: for RNA-TBDMS and 2 modified RNA chemistries
Pre-treatment: Trityl-on oligo sample preparation. Mix equal volume of loading

buffer with cleavage/deprotection solution
STEP 1
SPE Formats
60 mg/ 3 mL cartridges: < 200 nmole scale
Load crude oligo cocktail

All trityl-off impurities flow
directly through; no wash
required.
150 mg/ 3 mL cartridges: < 1 mole scale

5 g/ 60 mL cartridges: 5 25 mole scale
Loading of Crude Oligo
DCA
STEP 2
50 mg/ 96-well plate: 200 nmole scale per well
Detritylate
Less than 2 %
depurination observed.
A faint orange band
will appear at top half
of cartridge indicating
DMT retention.
96-well plate
Detritylate
Organic
Mixture
STEP 3
Negligible Depurination
Significant effort was made during
the development of Clarity QSP to
minimize the causes of depurination. The lower acid concentrations
and limited exposure times within
the protocol generate less than 2%
depurination.
Elute target oligo

pH buffered solutions used to
maintain safe pH for oligo;
select elution buffer based on
downstream requirements.
Full Length Trityl-On Oligo
Impurity
N-1 Sequence
Detritylated Failure Sequences
Trityl Group
Full Length Target Oligo
Elution of Target Oligo
304
Request a FREE copy of Technical Note TN-0008, Avoiding

Depurination During Trityl-on Purification for more information.
Phenomenex
U.S. Patent No. 7, 119, 145
Clarity QSP (cont'd)

High Purity, High Yield DNA and RNA
Clarity QSP is a next generation trityl-on purification solution that was specifically designed to complement contemporary synthetic
processes and consistently deliver high purities and recoveries for all types of synthetic DNA and RNA sequences. Complete discrimination between full-length trityl-on sequences from impurities is guaranteed. The final elution step delivers concentrated, full-length
sequences in a stable media suitable for in vivo applications and downstream analysis conducive for MS, NMR, CE, and HPLC.
53nt DNA Purification
High-Throughput DNA Purification
Sequence: ACAGTCGTACAGTCATATATTACTATTCAGTGTCTACTGCAGTCGTTATCTAT
Synthesis Scale: 200 nmole
Format: 50 mg / 1 mL
Sequence: GTGGATCTGCGCACTTCAGGCTCCTGGGCT
Synthesis Scale: 200 nmole
Format: 96-Well Plate (50 mg / well)
16406
25
mAU
25
20
15
20
15
10
10
0
2
10
12
14
16
18
min
mAU
25
mAU
25
15
10
15
10
0
10
12
14
16
18
min
mAU
20
15
10
5
App ID 16405
25
20
App ID 16406
20
mAU
25
20
15
10
5
0
0
2
10
12
14
16
18
min
1. Crude Trityl-on
2. Load fraction
3. Detritylated final elution
10
16
18
min
OD260
Load Fraction
Detritylated
Final Elution
Recovery
Purity
(Peak area)
6.51
29.6
89 %
93 %
39.7
14
1. Crude Trityl-on
2. Load fraction
OD260
Crude Trityl-on
12
Crude Trityl-on
28.3
Load Fraction
Detritylated
Final Elution
Recovery
Purity
(Peak area)
5.3
20.8
90.3 %
92 %
Crude 27nt RNA Purification (TBDMS Chemistry)

Sequence: Proprietary
Synthesis Scale: 1 mole
Format: 150 mg / 3 mL
clarity QSP | synthetic DNA/RNA | BIOSEPARATIONS
mAU
16408
mAU
40
30
20
10
0
mAU
40
30
20
10
mAU
40
App ID 16408
30
20
10
0
2
10
1. Crude Trityl-on
2. Load fraction
Crude Trityl-on
33.4
12
14
16
18
min
OD260
Load Fraction
Detritylated
Final Elution
Recovery
Purity
(Peak area)
9.22
22.9
94 %
84 %
Clarity QSP Well Plates & Cartridges
Part No.
Description
Formats
8E-S102-DGB Clarity QSP Well Plate
8B-S102-UBJ Clarity QSP Cartridge
8B-S102-SBJ Clarity QSP Cartridge
8B-S042-LFF Clarity QSP Cartridge
Buffers*
AL0-8279
Clarity QSP DNA Loading Buffer
AL0-8280
Clarity QSP DNA Loading Buffer
AL0-8281
Clarity QSP RNA-TBDMS
Loading Buffer
AL0-8282
Clarity QSP RNA-TBDMS
Loading Buffer
Unit
50 mg/well
60 mg/3 mL
150 mg/3 mL
5 g/60 mL
Price
1/box
50/box
50/box
16/box
100 mL
1L
100 mL
ea
ea
ea
1L
ea
* RNA-TOM loading buffer available upon request
For more information on the Clarity QSP protocol,

depurination, or applications, please request a copy
of the Clarity QSP User's Manual.
Phenomenex
Request Technical Note TN-0015

Comparing Performance of
High-Throughput, Trityl-on RNA/
DNA Purification Products to see
the benefits of using Clarity QSP
over other trityl-on solutions.
305

Quick, Simple Removal of Salt and
Reagent
70 % typical purity by removing salt and excess
reagent
80 % typical recovery of target oligo
BIOSEPARATIONS | synthetic DNA/RNA | clarity rp-desalting
For cleanup of trityl-off DNA and RNA sequences

Removes salt prior to MS analysis
Economical and disposable format
Clarity QSP, Oligo-WAX, and Oligo-RP can be used to yield
highly purified target oligonucleotides (> 85 % purity) from a
synthesis mixture. For simple desalting and reagent removal of
a trityl-off synthetic oligonucleotide, Clarity RP-Desalting tubes
can be used. Clarity RP-Desalting tubes are a poly-functional
silica-based C18 sorbent that provides a high capacity, fast and
effective desalting process.
Crude DNA Desalting

Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Sample:
Desalting of Dye-Labeled DNA

Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Sample:
Clarity 3 m Oligo-RP C18

50 x 4.6 mm
00B-4441-E0
A: 50 mM TEAA, pH 7.5 / 5 % Acetonitrile
B: Methanol
A/B (90:10) to A/B (40:60) in 20 min
1 mL/ min
UV @ 260 nm
25nt DNA oligonucleotide

50 x 4.6 mm
00B-4441-E0
A: 50 mM TEAA / 5 % Acetonitrile
B: Methanol
A/B (90:10) to A/B (40:60) in 20 min
1 mL/ min
UV @ 260 nm
40nt DNA
16022
mAU
200
Crude
150
100
50
15991
mAU
mAU
80
Crude
60
2.5
7.5
10
12.5
15
10
17.5
min
17.5
min
17.5
min
Load
40
20
0
0
mAU
80
-5
60
mAU
Load
40
2.5
7.5
10
12.5
15
10
20
Wash
0
mAU
80
60
Wash
20
mAU
80
Final Purity: 71 %
Final Recovery: 94 %
60
40
20
Final Elution
App ID 15991
2.5
7.5
10
12.5
15
500
Final Elution
400
300
200
100
0
0
2.5
7.5
10
12.5
15
17.5
min
App ID 16022
40
0
0
2.5
7.5
10
12.5
15
17.5
A quencher-labeled sample of DNA (25nt) with the sequence FAMTTGACTTAGACTTAGACTTAGTTT was desalted using Clarity RP-Desalting tubes in the 200 mg/3 mL format.
Collection fractions were then analyzed for purity and recovery using the above protocol.
min
For more information on the Clarity products please

contact your Phenomenex technical consultant.
306
Phase
200 mg/3 mL*

50/box
500 mg/3 mL**

50/box
C18
8B-S041-FBJ
8B-S041-HBJ
* For 200 mole synthesis

** For 1 mole synthesis
Phenomenex
Clarity Oligo-MS HPLC Columns

Rapid and Efficient LC/MS Separation for
QC and Characterization
Core-shell particle technology provides improved speed,
resolution, and sensitivity
2.6 m particles deliver increased efficiency at reduced
backpressures
Easily transfer quantitative LC/MS methods to any system
with 2.6 m particles
Clarity Oligo-MS, C18 columns have been engineered for the MS

characterization of synthetic DNA and RNA samples. This media
is based on core-shell technology which generates extremely high
efficiencies due to the innovative particle design. This increase in
efficiency improves the resolution between critical oligo sequences,
gives higher sensitivity for easier MS quantitation, and allows for a
decrease in column length for higher throughput.
Poly dT Standard (12-18mer)

Column:
Dimensions:
Part No.:
Mobile Phase:

50 x 2.0 mm
00B-4479-BO
A/B (95:5) to A/B (80:20) in 10 min
0.5 mL/min
50 C
UV @ 260 nm (22 C)
20 L
Poly dT (12-18)
Gradient:
Flow Rate:
Temperature:
Detection:
Injection Volume:
18836 Sample:
mAU
80
60
40
App ID 18836
Core-Shell Technology for Synthetic DNA/RNA Analysis

Clarity Oligo-MS media is not fully porous like traditional particles
used for the analysis of oligonucleotides. It is a core-shell particle
technology which uses a sol-gel processing technique to grow a
homogeneous porous shell onto a solid core. This highly optimized
process combined with uniform particle size distribution produces
a column that generates extremely high plate counts.
20
0
1.7 m
Core-Shell Particle
10
12
min
clarity oligo-ms | synthetic DNA/RNA | BIOSEPARATIONS
1.7 m particles boost performance of existing sub-2 m

methods
Achieve Baseline Resolution of N-1 and N+1 Oligo

from Target
The high plate counts generated by the Clarity Oligo-MS material
produce extremely high efficiencies and thus excellent resolution between oligonucleotides of similar length and structure.
Scientists can achieve baseline resolution between synthetic
oligonucleotides with just one base difference allowing easier
quantitation.
Reduced diffusion path maximizes efficiency

Increased efficiencies compared to traditional
fully porous sub-2 m columns. Typical operating
backpressures > 400 bar
1.25 m Solid Core
0.23 m Porous Shell
Rapid Separation of Complex Oligo Samples

Due to the high resolving power of Clarity Oligo-MS columns,
high-throughput methods for the separation of complex synthetic
mixtures can be developed. Using short (50 mm length) columns,
impurities are separated from the peak of interest in less than 12
minutes.
Crude DNA 30mer
1.7 m
Column:
Dimensions:
Part No.:
Mobile Phase:
2.6 m
Core-Shell Particle
Reduced diffusion path maximizes efficiency
Ultra-high performance on any system with
Clarity Oligo-MS 2.6 m columns
Gradient:
Flow Rate:
Temperature:
Detection:
Injection Volume:
Sample:

50 x 2.0 mm
00B-4479-BO
A/B (95:5) to A/B (80:20) in 10 min
0.5 mL/min
50 C
UV @ 260 nm (22 C)
20 L
Crude DNA 30mer
18837
mAU
0.35 m Porous Shell

1.9 m Solid Core
60
App ID 18837
40
2.6 m
20
Phenomenex
0
0
10
12 min
307
Clarity Oligo-RP HPLC Columns

Reversed Phase LC for Purification
and Characterization
Easily separate N-1 failure sequences from target oligo
with > 90 % purities
Trityl-off purification of DNA, RNA, Thioates, and
modified/labeled oligonucleotides
Purify oligos up to 60 mer in length

Excellent column for reversed phase HPLC quality control
(QC) testing
Clarity Oligo-RP has been specifically designed for the reversed
phase purification of oligonucleotides with balanced hydrophobicity
and polar selectivity. The media is based on composite particle
TWIN technology. This technology gives improved selectivity
and efficiency for oligonucleotides when compared to other
hybrid, polymer, and silica particles found in the marketplace. It is
available in 3, 5, and 10 m particle sized beads and in a variety of
dimensions.
Preparative Purification on Oligo-RP
Reversed phase separation of oligonucleotides has advantages over
other modes of separations such as ion-exchange. The Oligo-RP
phase allows high loadability and delivers high recovery and purity,
eliminating the need for extra purification steps. This is achieved
through an ion-pair separation of the trityl-off oligonucleotide from
failure products and other impurities.
DNA Purification
(A) Preparative (B) Analytical QC
Gradient:
Flow Rate:
Detection:
Sample:
16021

50 x 4.6 mm
00B-4441-E0
A: 50 mM TEAA pH 7.5
B: Methanol
10 % to 45 % B in 30 minutes
1 mL/ min
UV @ 260 nm
1. 40nt DNA with sequence
CTTCTGAACAGTTGATCTATGCACTTCAGACTTATGATCA (2.5 g)
2. 39nt DNA with sequence
TTCTGAACAGTTGATCTATGCACTTCAGACTTATGATCA (2.5 g)
40nt
mAU
39nt
100
80
60
40
20
10
15
min
Fingerprint of 40mer DNA

Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Sample:
(A)
App ID 15947
800
600
400
200
15970

50 x 4.6 mm
00B-4441-E0
A: 50 mM TEAA pH 7.5 / 5 % Acetonitrile
B: Methanol
20 % to 25 % B in 20 minutes; hold at 5 minutes @ 25 % B
1 mL/ min
UV @ 260 nm
40nt DNA with sequence
5-CTC CTG GGC AGT GGA TCT GCG CACTTC AGG CTC CTG GGC A-3
mAU
39
2.5
7.5
10
12.5
15
17.5
min
A 200 g (1 mole) 20mer DNA sample was loaded onto a 10 mm ID Clarity

Oligo-RP column. Impurities were separated from the target sequence.
140
38
800
700
37
35 36
600
31
32 33
34
500
(B)
20
400
App ID 15970
15948
mAU
160
300
App ID 15948
120
100
80
60
40
20
0
200
100
0
0
2.5
7.5
10
12.5
15
17.5
10
20
30
min
min
A Clarity Oligo-RP analytical column was used to verify the purity of

the preparative purification. A purity of 92 % with a yield of 85 % was
determined.
308
20
Clarity Oligo-RP successfully separates a 40mer from a 39mer DNA

oligonucleotide due to its excellent efficiency and resolving power.
15947
1000
Column:
Dimensions:
Part No.:
Mobile Phase:
Column: Clarity 3 m Oligo-RP C18

Dimensions: (A) 50 x 10.0 mm
(B) 50 x 4.6 mm
Part No.: (A) 00B-4441-N0
(B) 00B-4441-E0
Mobile Phase: A: 50 mM TEAA pH 7.5/ 5 % Acetonitrile
B: Methanol
Gradient: 10 % to 60 % B in 20 minutes
Flow Rate: (A) 4.7 mL/ min
(B) 1.0 mL/ min
Sample: 20nt DNA
mAU
DNA Purification of Failure N-1 from Target N Sequence
App ID 16021
BIOSEPARATIONS | synthetic DNA/RNA | clarity oligo-RP
Preparative dimensions and particle sizes for loads

> 5 mole
Separate N-1 Failure Sequences from Target N Sequence

The Oligo-RP sorbent is specifically designed to accommodate all
possible interactive features of nucleosides with matching modes of
reactivity to its own. The sorbent possesses hydrophobic, dipolar,
-, and hydrogen bond donor/acceptor sites; this combination of
interaction along with an ion-pairing reagent elicits a high degree of
differential selectivity between nucleic acids. Thus it can recognize
even the slightest changes in nucleotide sequence, such as a
difference of one base (N and N-1) or substitution of one base for
another.
Due to the high efficiency of the sorbent and ion-pairing interactions,

a fingerprint of a crude 40mer DNA on Clarity Oligo-RP is produced
illustrating baseline resolution of impurities from the final product.
Phenomenex
Clarity Oligo-WAX LC Columns

High Purity, High Loadability Preparative
Ion-Exchange Purification
High loading capacity due to very high density ligand
Increase productivity by running at higher flow rates and
pressures
Clarity Oligo-WAX LC columns were designed with the synthetic
DNA/RNA preparative chromatographer in mind. Oligo-WAX is an
advantageous combination of purity, capacity, mechanical strength,
cost, and efficiency.
Tailored for Preparative Purification
The majority of synthetic oligo preparative purifications are performed using a strong anion exchanger bonded to a 10 or 15 m
polymer backbone. Polymer backbones are amenable to clean in
place protocols and strong anion exchangers have a wide effective pH range. To date, these technologies have been satisfactory
for prep purifications and will continue to be. However, due to the
fact that Clarity Oligo-WAX is a cross-linked weak anion exchanger
bonded to a 10 m high purity silica, this technology offers advantages such as high loading capacity, excellent peak efficiency, and
a robust backbone that aren't available with typically used purification products.
DNA Purification of N-1 Sequence from Target N Sequence

Column:
Dimensions:
Part No.:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Sample:
16398
Clarity 10 m Oligo-WAX
150 x 4.6 mm
00F-4451-E0
A: Water
B: Acetonitrile
C: 100 mM Tris, pH 8
D: 2 M Sodium chloride
A/B/C/D (70:10:20:0) to (10:10:20:60) in 20 min
2.2 mL/min
UV-Vis Abs.-Diode Array (ambient)
Depurinated A & G and 20mer DNA
mAU
19nt
20
20nt
clarity oligo-wax | synthetic DNA/RNA | BIOSEPARATIONS
Columns amenable to HPLC and FPLC systems
Purify Failure Sequences and Contaminants from Target

Sequence
Ion-exchange is an excellent separation mode for purifying
contaminants and failure sequences from target sequences.
Clarity Oligo-WAX, due to its increased efficiency compared
to other ion-exchange columns, has the ability to recognize
minute charge differences in nucleotide sequences such as
failure sequences or base substitutions.
App ID 16398
Excellent efficiency column results in > 90 % purities due to

good fractionation of closely eluting compounds
0
0
10
12
14
16
18
min
CE Purity Analysis of Ion-Exchange Purification
GE Healthcare SOURCE 15Q

150 x 10 mm (self-packed)
Final Purity = 88.8 %, N-1 = 2.0 % Final amount = 205.9 ODs

Recovery of full-length product = 28.9 % Conductivity = 200 S/cm
Clarity Oligo-WAX 10 m
150 x 10 mm (pre-packed)
Final Purity = 95.1 %, N-1 = 1.3 % Final amount = 188.9 ODs

Recovery of full-length product = 28.4 % Conductivity = 151 S/cm
Two purification runs were performed on each column with fractional QC being taken after each run. Passing fractions from the two purification runs
were combined into one pooled lot for each column. That pooled lot was then divided equally and run through a Clarity desalting tube. Final ODs
and QC were taken after desalting, including ESI, CE, and conductivity. The purity and resolution of Clarity Oligo-WAX was considerably better than
SOURCE 15Q. Though SOURCE had a slightly higher recovery of full length oligo, it was not a wide enough margin to offset the purity advantage.

Phenomenex
Data courtesy of a large, Iowa-based oligo manufacturer.

309
Clarity Oligo-MS, Oligo-RP, and Oligo-WAX LC Columns

Ordering Information sds
Minibore Columns (mm)
Phase
50 x 2.0
100 x 2.0
150 x 2.0
00D-4441-B0
100 x 2.1
00F-4441-B0
5 m Oligo-RP C18
Phase
00B-4441-B0
50 x 2.1
1.7 m Oligo-MS C18
00B-4480-AN
00D-4480-AN
2.6 m Oligo-MS C18
00B-4479-AN
00D-4479-AN
BIOSEPARATIONS | synthetic DNA/RNA | claritY
3 m Oligo-RP C18

Phase
50 x 4.6
100 x 4.6
00F-4442-B0
150 x 4.6
2.6 m Oligo-MS C18
00B-4479-E0
00D-4479-E0
3 m Oligo-RP C18
00B-4441-E0
5 m Oligo-RP C18
00B-4442-E0
00D-4441-E0
00F-4445-E0
00D-4451-E0
00F-4451-E0
10 m Oligo-RP C18
10 m Oligo-WAX
Semi-Prep Columns (mm)

Phase
50 x 10.0
3 m Oligo-RP C18
00B-4441-N0
100 x 10.0
5 m Oligo-RP C18
00B-4442-N0
00D-4442-N0
10 m Oligo-RP C18
10 m Oligo-WAX
Prep and Axia-Packed Preparative Columns (mm)

Phase
100 x 21.2
150 x 21.2
5 m Oligo-RP C18 00D-4442-P0-AX
10 m Oligo-RP C18
00F-4445-P0-AX
10 m Oligo-WAX
00F-4451-P0-AX
00F-4441-E0
00F-4442-E0
SecurityGuard
Cartridges (mm)
4 x 2.0*
/10pk
AJ0-8134
/10pk
AJ0-8134
for ID: 2.0-3.0 mm
250 x 4.6
00G-4442-E0
150 x 10.0
250 x 10.0
00F-4442-N0
00G-4442-N0
00F-4445-N0
00G-4445-N0
00F-4451-N0
00G-4451-N0
250 x 21.2
00G-4442-P0-AX
00G-4445-P0-AX
00G-4451-P0-AX
Bulk material available upon request.
150 x 30
aufInquire
Anfrage
00F-4445-U0-AX
auf Anfrage
00F-4451-U0-AX
Evaluate Clarity BioSolutions in

your lab for 45 days, if you are not
completely satisfied return it for a
full refund.
SecurityGuard
Cartridges (mm)
4 x 3.0*
/10pk
AJ0-8135
/10pk
AJ0-8135
/10pk
AJ0-8135
/10pk
AJO-8324
for ID: 3.2-8.0 mm
SecurityGuard
Cartridges (mm)
10 x 10
/3pk
AJ0-8136
/3pk
AJ0-8136
/3pk
AJ0-8136
/3pk
AJ0-8325
for ID: 9-16 mm
250 x 50
aufInquire
Anfrage
00G-4445-V0-AX

15 x 21.2**
15 x 30.0
/ea
/ea
AJ0-8210
AJ0-8310
/ea
/ea
AJ0-8210
AJ0-8310
/ea
/ea
AJO-8639
AJO-8420
for ID: 18-29 mm
30-49 mm
*SecurityGuard Analytical Cartridges require universal holder Part No.: KJO-4282

KrudKatcher Ultra In-line Filter

Disposable in-line filter fits virtually all UHPLC / HPLC
columns 1.0 to 4.6 mm ID. Extremely low dead
volume minimizes sample peak dispersion.
Pressure rated to 1375 bar (20,000 psi).
(see p. 17 for more information)
KrudKatcher Ultra In-line Filter
Part No.
Description
AF0-8497
KrudKatcher Ultra In-Line Filter,
0.5 m Depth Filter x 0.004 in. ID
Unit
3/pk
Price
KrudKatcher Ultra requires 5/16 in. wrench. Wrench not provided. See p. 373
310
Phenomenex
Chiral LC
CHIRAL LC
Chiral LC
Chiral LC Column Types.................................................................... 312

Ceramospher and Chiral CD-Ph............................................................ 313
Chiral Column Protection Systems........................................................ 317
Chirex..................................................................................................... 314
Hypercarb.............................................................................................. 317
Lux......................................................................................................... 318
Merck KGaA, Darmstadt, Germany, Products
ChiraDex (b)...................................................................................... 324

Whelk-O 1......................................................................................... 324
Nucleosil
Nucleosil Chiral................................................................................. 324
Nucleodex b...................................................................................... 324
Resolvosil BSA-7.............................................................................. 324
Shodex ORpak...................................................................................... 325
Sumichiral OA........................................................................................ 326
Ultron ES................................................................................................ 325
311 - 326
Chiral LC Column Types

LC Chiral Stationary Phase (CSP) Classification Diagram
Type
Description
Chemistry
Mechanism
Brands
Low molecular weight chiral selectors

Ionic or covalent bonding
Attractive interactions
Hydrogen bonding
Charge transfer (- interaction)
Dipole stacking
Chirex
Nucleosil Chiral
Sumichiral OA
Whelk-O-1
Cellulose and amylose derivatives

Poly methacrylates
Poly acrylamides
Attractive interactives
Insertion complexes
Lux Cellulose and Amylose
Brush (Pirkle)
II
Helical Polymers
III
Cavity
Cyclodextrins, Crown ether
Inclusion complexes
IV
Ligand Exchange
Amino acid-metal complex
Diastereomeric metal complex
Protein
a-acid glycoprotein
Bovine Serum Albumin
Hydrophobic interactions
Polar interactions
Antibiotics
Glycopeptides
Hydrogen bonding
Charge transfer (- interaction)
Inclusion complexation
Ionic interactions
Peptide bonding
VI
Macrocyclic
Other Types
Carbon-Based (Hypercarb) and Ceramic-based (Ceramospher)
Type I
Chiral CD-Ph
ChirDex b
Nucleodex b
Sumichiral OA
Chirex
Nucleosil Chiral
Sumichiral OA
ORpak
Resolvosil BSA
Ultron ES
None
Type II
Silica
chiral lc column types
Chiral moiety
Achiral Spacer
A
Natural:
OH
OH
OH
O HO
HO
OH
O
O HO
OH
chiral lc
Silica
Example: linear poly (1 - 4 - - D -glucose)
Example: Chirex 3001
Type III
Type IV
-Cyclodextrin
-Cyclodextrin
-Cyclodextrin
OH
H
O
H
O
H
O
O
HO
HO
HO
OH
HO
O
O
H
O
H
O
HO
HO
O
H
HO
OH
HO
O
O
H
HO
HO
HO
O
OH
OH
OH
OH
X
C
Silica
HO
H
O
HO
O
O
H
O
OH
OH
HO
X
C
HO
OH
HO
HO
HO
Silica
OH
HO
O
H
HO
O
H
H
O
O
HO
HO
HO
H
O
HO
O
HO
HO
HO
HO
OH
O
O
OH
OH
Me
R1
R2
Schematic showing the reversible inclusion complex formation
Type V
312
Phenomenex
Ceramospher and Chiral CD-Ph
Ceramospher
Chiral Columns
Chiral CD-Ph Columns

Made of precisely classified high-purity silica, this versatile
chiral stationary phase is modified with phenylcarbamated
-cyclodextrin.
High-efficiency, pressure-stable
ceramic-based materials
Suitable for separation of basic, neutral,

and amphoteric optical isomers
Choice of normal or aqueous mobile

phase conditions
Usable with both aqueous and non-aqueous

mobile phases
Enantioselectivity for acidic, basic and

neutral chiral compounds
High durability
High loadability combined with long

column lifetimes
High sample loadability
RU-1. Used for normal phase separations of a wide variety of

chiral compounds, typical mobile phases include: 1 % isopropanol-amine or dimethylamine in alcohol (methanol or ethanol)
for basic compounds, and 1 % acetic acid in alcohol for acidic
compounds. Retention can be controlled by adding small percentages of hexane to these eluents.
Shiseido Ceramospher RU-1

250 x 4.6 mm x 2 columns
CH0-3905
Triethylamine/Methanol (1:100)
1.0 mL/min
50 C
UV @ 254 nm
=2.22
O
NHCH3
Cl
Laudanosine
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
10
20
30 min
Shiseido Ceramospher RU-2

250 x 4.6 mm
CH0-3907
Diethylamine/Water/Acetonitrile (0.1:10:90)
0.5 mL/min
50 C
UV @ 254 nm
|
chiral lc
RU-2. Treating the base material with a hydrophobic agent imparts excellent stability under aqueous conditions. Water is typically combined with methanol or acetonitrile in the mobile phase.
When the content of water in the eluent is 30 % or higher, the
retention mechanism appears to be based on typical reversed
phase partitioning; when water makes up 5 % or less of the eluent, separations appear to be based on normal phase partitioning. Because of the long lifetime expected, this material is well
suited for preparative use.
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Ceramospher and Chiral CD-PH
Ceramospher Phases
Ketamine
App ID 5237
Based on 5 m 40 spherical sodium magnesium silicate particles, Ceramospher phases RU-1 and RU-2 are novel materials
for chiral HPLC separations. Enantiomer resolution is accomplished on an ion-exchange adduct of the base clay material in
combination with an optically-active metal complex (based on
ruthenium).
=1.60
CH3O
N
CH3
CH3O
CH2
App ID 5236
Call Phenomenex for a complete listing of

Ceramospher chiral applications.
OCH3
OCH3
Atropine
Column:
Dimensions:
Part No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Injection Vol.:
Phenomenex
10
15
20
25 min

250 x 4.6 mm
CH0-5862
0.5 mol/L NaClO4/CH3CN (70:30)
0.5 mL/min
25 C
UV @ 220 nm
10 L
Price
CH 3
*
2OH
OCOCHCH
App ID 13576
Ceramospher and Chiral CD-Ph Chiral Columns
Part No.
Mfr. No.
Dimensions (mm)
RU-1
CH0-3904
50503
150 x 4.6
CH0-3905
50504
250 x 4.6
RU-2
CH0-3906
50603
150 x 4.6
CH0-3907
50604
250 x 4.6
Chiral CD-Ph
CH0-5862
80054
250 x 4.6
12
16
20
24
28
32 min
313
Chirex
High enantioselectivity
Fast run times
Rugged, long-lived columns
Easy scale-up to preparative
Allow direct/indirect resolution of enantiomeric amines,
amino acids, hydroxy acids, alcohols, carboxylic acids,
ketones, ethers, and esters
Chiral separations are extremely important to the pharmaceutical
and biotechnology industries, as well as most other areas of natural products chemistry. Optically active therapeutic drugs require
selective and sensitive techniques. Government regulations also
continue to spur and require the development of rapid, accurate
and reproducible methods for the analysis and purification of
enantiomeric compounds.
Phenomenex meets these challenges with Chirex brand HPLC columns. Chirex is available in 11 different stationary phases. These
chemically rugged, versatile columns are used for the direct and
indirect resolution of enantiomeric amines, alcohols, carboxylic acids, hydroxy acids, amino acids, ketones, lactones, ethers, esters,
and other biologically active compounds.
The challenge is to provide selective yet versatile HPLC columns for

both trace analysis and the purification of bulk drug.
Hundreds of applications demonstrate the performance
of Chirex phases for a multitude of phamaceutical and
agrochemical compounds. For a complete list, please
contact your Phenomenex technical consultant
chirex
Which Chirex Stationary Phase?

Stationary phase selection depends on presence/absence of
chemical groupings in the chiral molecule.
Chirex Column Selection Guide

Presence of Chemical Groupings in Chiral Molecule
Recommended Columns:
chiral lc
Class
Group 1
Group 2
Group 3
Group 4
Group 5
Group 6
Group 7
Group 8
Group 9
Group 10
Aromatic N
Y
Y
Y
Y
Y
Y
Y
Y
Y
COOH OH
Other Comment
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
First Choice
Aromatic -amino acids, -hydroxy acids 3126

3022 or 3020
3014 or 3020
3005
3001 or 3014
3001
Aliphatic -amino acids, -hydroxy acids 3126
and their derivatives
3126
3014
Asymmetric other than carbon.
3014
Chiral center at N,S,P,B, etc
Second Choice
Third Choice
3005 or 3001
3014
3022
3010
3005
3005
3011 or 3012
3018
3018
3001
3020 or 3022
3019 or 3020
3010
3019 or 3020
3010
3001
3001
3005
For more information on Chirex chiral stationary phase

descriptions, contact your Phenomenex technical representative.
Chirex is a trademark of Chirex, Inc. licensed to Phenomenex.
314
Phenomenex
Chirex
Chiral HPLC of Amino Acids

Pirkle-concept and Ligand Exchange type columns
High enantioselectivity
Chirex HPLC columns are an excellent choice for underivatized and

derivatized amino acids.
Excellent efficiency
Separations of Amino Acid Derivatives
Separations of Underivatized Free Amino Acids
Separation
Factor ()
App ID No
14004
14080
14082
14038
14046
14027
14049
14019
13785
13790
14034
14085
14066
13750
14047
14022
14079
14081
13745
14035
14009
14083
14018
14024
14457
14456
13789
14029
14041
13740
13748
14031
14011
14016
14043
13787
13737
13743
14006
chiral lc
Separation
Factor ()
1.66
2.26
1.62
1.67
1.19
2.15
1.10
1.42
1.23
1.36
1.80
2.47
2.77
1.22
1.11
1.71
1.78
1.69
1.32
1.70
1.56
1.36
1.83
1.42
1.59
1.18
1.42
1.95
1.38
1.44
1.78
1.77
2.50
1.17
1.20
1.15
1.11
1.34
1.91
Compound
Chirex Phase
Alanine
3126
Alanylglycine
3126
Alanylglycyl-glycine
3126
Alloisoleucine
3126
Allothreonine
3126
Arginine
3126
Asparagine
3126
Aspartic acid
3126
Baclofen
3126
p-Boronophenylalanine
3126
2-amino-n-Butyric acid
3126
Cystine
3126
2,6-Diaminopimelic acid
3126
3-(3,4-Dihydroxyphenyl)-alanine (DOPA)
3126
Glutamic acid
3126
Glutamine
3126
Glycylalanine
3126
Glycylvaline
3126
Histidine
3126
Isoleucine
3126
Leucine
3126
Leucylglycyl-glycine
3126
Lysine
3126
Methionine
3126
3126
a-Methyl Leucine
3126
a-Methyl Tryptophan
Naphthylglycine
3126
Norvaline
3126
Ornithine
3126
Phenylalanine
3126
Phenylglycine
3126
Pipecolic acid
3126
Proline
3126
Serine
3126
Threonine
3126
dl-Threo-3-phenylserine
3126
Tryptophan
3126
Tyrosine
3126
Valine
3126
Chirex
Compound
Chirex Phase
App ID No.
t-BOC-Derivatives (Butyloxycarbonyl)
t-BOC-Leucine
3012
1.09
14064
t-BOC-Phenylalanine
3012
1.09
13784
t-BOC-Valine
3012
1.10
14063
N-FMOC Derivatives (9-Fluorenylmethyloxycarbonyl)
N-FMOC-Leucine
3011
1.20
13800
N-FMOC-Phenylalanine
3011
1.10
13796
N-FMOC-Valine
3011
1.12
13798
Z-Derivatives (Benzyloxycarbonyl)
Z-Alanine
3011
1.16
13729
Z-Asparagine
3010
1.12
13760
Z-Leucine
3011
1.17
13731
Z-Norvaline
3011
1.13
13755
Z-Phenylalanine
3012
1.08
13762
Z-Serine
3011
1.09
13758
Z-Valine
3011
1.13
13753
N-Acetyl Derivatives
N-Acetylalanine
3126
1.17
14052
N-Acetylleucine
3126
1.39
14058
N-Acetylmethionine
3126
1.27
13728
N-Acetylvaline
3126
1.50
14055
N-Formyl Derivatives
N-Formylvaline
3126
1.37
13721
N-Formylmethionine
3126
1.25
13722
N-Benzoyl Derivatives
N-Benzoylglutamic acid
3012
1.14
13782
N-Benzoylleucine
3012
1.11
14460
N-Benzoylphenylalanine
3012
1.17
13730
N-Benzoylphenylglycine
3012
1.13
14461
N-Benzoylvaline
3012
1.19
13778
N-Dansyl Derivatives (5-5-Dimethyl-aminonaphthalene-1-sulfonyl derivative)
N-Dansylnorvaline
3011
1.24
13766
N-Dansylphenylalanine
3011
1.27
13771
N-Dansylthreonine
3012
1.18
13734
N-Dansyltryptophan
3010
1.15
13774
N-Dansylvaline
3011
1.28
13763
PTH Derivatives (Phenylthiohydantoin)
PTH-Valine
3014
1.12
13921
Alpha ()= Separation Factor = k2/k1
Separation potential of some other amino acid derivatives: (Recommended columns: Chirex 3010, 3011, 3012, 3014)
CBZ-Derivatives (carbobenzoxy; benzyloxycarbonyl); IC-Derivatives (phenylisocyanate);
Dabsyl Derivatives (4-4-dimethylaminoazobenzene-4'-sulfonyl)
Z-Alanine
Baclofen
Column:
Dimensions:
Part No.:
Mobile Phase:
Chirex 3011
250 x 4.0 mm
00G-3011-D0
0.01 M Ammonium Acetate in
Methanol
13729
Detector: UV @ 254 nm
Column:
Dimensions:
Part No.:
Mobile Phase:
Chirex 3126
150 x 4.6 mm
00F-3126-EO
2 mM Copper (II) sulfate in water /
Isopropanol (85:15)
Phenomenex
=1.23
App ID 13729
=1.16
10
15 min
App ID 13785
13785
10 min
315
Chirex
5 m Starter Columns (mm)
Phase Chirex Phase Description Bond Type Linkage Type
3001
3010
3011
3014
3018
3020
3022
3005
3126
(R)-PGLY and DNB

(S)-VAL and DNAn
(S)-LEU and DNAn
(S)-VAL and (R)-NEA
(S)-PRO and (R)-NEA
(S)-LEU and (R)-NEA
(S)-ICA and (R)-NEA
(R)-NGLY and DNB
(D)-Penicillamine
Preparative Columns and Bulk Media are available in 15

and 30 m particle sizes. Call for information on pricing
and availability. Detailed notes on Care and Use, as well as
performance testing, are provided with each column.
50 x 4.6
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
Ion-Metal
Amide
Urea
Urea
Urea
Urea
Urea
Urea
Amide
Lig Exchange
00B-3001-E0
00B-3010-E0
00B-3011-E0
00B-3014-E0
00B-3018-E0
00B-3020-E0
00B-3022-E0
00B-3005-E0
00B-3126-E0
Covalent
Urea
00B-3012-E0
For Chiral Column Performance Check Standards, see p. 366.
For In-line Filters specifically designed to protect your chiral

column investment, see p. 317
For HPLC Column Chiller/Heater System (8-70 C), see p. 357.
3012
(R)-PGLY and DNAn
chiral lc
Chirex / Chiral Method Development kits
5 m Analytical and Guard Columns (mm)

Phase
Chirex Phase Description
Amide
Urea
Urea
Urea
Urea
Urea
Urea
Urea
Urea
3005
(R)-NGLY and DNB
Covalent
Amide
00F-3020-B0
3126
(D)-Penicillamine
Ion-Metal
Lig Ex
00F-3126-B0
00G-3001-E0
00G-3010-E0
00G-3011-E0
00G-3012-E0
00G-3014-E0
00G-3018-E0
00G-3019-E0
00G-3020-E0
00G-3022-E0
03A-3001-E0
03A-3010-E0
03A-3011-E0
03A-3012-E0
03A-3014-E0
03A-3018-E0
03A-3019-E0
03A-3020-E0
03A-3022-E0
00G-3005-B0
00F-3005-E0
00G-3005-E0
03A-3005-E0
00G-3126-B0
00F-3126-E0
00G-3126-E0
03A-3126-E0
Kit B. For -Amino Acids, -Hydroxy

Acids, Carboxylic Acids, Aromatic
Amines, Alcohols and Amino Alcohols
Chiral Method Development

Kits
Kit A. For Pharmaceuticals,
Agrochemicals
The broad range of Pirkle-concept (Type I, or brush type) columns
carefully chosen for this Kit will enable the chiral chromatographer
to quickly and easily survey stationary phase utility and separation
conditions for a wide variety of enantiomeric compounds. Used
with normal phase solvent systems, these chiral columns are highly
efficient tools particularly well-suited for enantiomeric separations
of pharmaceuticals and agrochemicals.
This Kit contains a collection of chiral columns for method development of enantiomeric -amino acids, -hydroxy acids, dipeptides, and aromatic amines, alcohols and amino alcohols. The kit
includes four Pirkle-concept columns and one ligand-exchange
type column. The Pirkle-concept columns are chosen for their high
utility/versatility in separating derivatized amino acids, whereas the
ligand-exchange column confers excellent enantioselectivity for
underivatized -amino acids, and -hydroxy acids.
Kit B. For -Amino Acids, -Hydroxy Acids,

Carboxylic Acids, Aromatic Amines, Alcohols and
Amino Alcohols
Kit A. For Pharmaceuticals, Agrochemicals

This kit contains five columns, 50 x 4.6 mm:
Column 5:
00F-3001-E0
00F-3011-E0
00F-3012-E0
00F-3020-E0
00F-3022-E0
00G-3001-B0
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
Column 3:
Column 4:
Guards
30 x 4.6
150 x 2.0
(R)-PGLY and DNB

(S)-VAL and DNAn
(S)-LEU and DNAn
(R)-PGLY and DNAn
(S)-VAL and (R)-NEA
(S)-PRO and (R)-NEA
(S)-LEU and (S)-NEA
(S)-LEU and (R)-NEA
(S)-ICA and (R)-NEA
Column 2:
250 x 4.6
Linkage Type
3001
3010
3011
3012
3014
3018
3019
3020
3022
Column 1:
Analytical
250 x 2.0
150 x 4.6
Bond Type
Chirex 3001: 3,5-dinitrobenzoic acid derivative of

(R)-phenylglycine
Chirex 3005: 3,5-dinitrobenzoic acid derivative of
(R)-1-naphthylphenylglycine
Chirex 3010: 3,5-dinitroaniline derivative of (S)-valine
Chirex 3014: (R)-1-(-naphthyl)ethylamine derivative
of (S)-valine
Chirex 3020: (R)-1-(-naphthyl)ethylamine derivative of
(S)-tert-leucine
This kit contains five columns, 50 x 4.6 mm:
Column 1:*
Column 2:*
Column 3:*
Column 4:
Column 5:
Chirex 3010: 3,5-dinitroaniline derivative of (S)-valine

Chirex 3011: 3,5-dinitroaniline derivative of (S)-tert-leucine
Chirex 3012: 3,5-dinitroaniline derivative of (R) phenylglycine
Chirex 3014: (R)-1-(-naphthyl)ethylamine derivative of
(S)-valine
Chirex 3126: Ligand-Exchange type based on (N,S) dioctyl(D)-penicillamine complexed with copper(II)
* Especially useful for derivatized amino acid analysis (see p. 315)
Chiral Method Development Kits

Part No.
Description
KH0-1892
Chiral Method Development (Kit A)
(If columns purchased separately price= 2.575)
316
Unit
ea
Price
Chiral Method Development Kits

Part No.
Description
KH0-1893
Chiral Method Development (Kit B)
Unit
ea
Price
(If columns purchased separately price= 2.575)
Phenomenex
Chiral Column Protection Systems

The following in-line filters are recommended to protect your valuable
Chiral HPLC column investment from damaging microparticulates.
Select the appropriate in-line filter by matching to your columns
diameter as indicated.
Chiral Column Protection Systems
Part No.
In-Line Filter Description
AF0-8497
HPLC KrudKatcher Ultra Column In-Line Filter,
0.5 m Porosity x 0.004 in. ID
Comment
Direct connect
Column Diameter Range

1 - 4.6 mm ID
Unit
3/pk
AF0-8420
HPLC SemiPrep Column In-Line Filter,

2.0 m Porosity x 10 mm dia., Biocompatible
Requires column coupler

AQ0-1393
6.0 - 16.0 mm ID
ea
AF0-7866
HPLC PREP Column In-Line Filter, S.S.,

Requires column coupler

AQ0-8374
18.0 - 30.0 mm ID
ea
Price
chiral column protection / Hypercarb / chiral LC
If you need assistance selecting the appropriate In-Line Filter,

please contact your Phenomenex Technical Consultant.
For Column Couplers, see p. 363
Hypercarb
|
chiral lc
Packing Material*
Particle
Shape/Size*
(m)
Pore
Size*
()
Pore
Volume
(mL/g)
Surface
Area*
(m2/g)
Carbon
Load*
%
Calculated Bonded
Phase Coverage
(mole/m2)
End
Capping*
Hypercarb
Spher. 3, 5,7
250
0.7
120
100
n/a
* Specifications above taken from 2006-2007 (then) Thermo Electron Corporation catalog.
5 m Columns (mm)
Part No.
Mfr. No.
CH0-3301
35005-104630
CH0-3302
35005-103030
CH0-3303
35005-102130
Description
Hypercarb
Hypercarb
Hypercarb`
Size (mm)
100 x 4.6
100 x 3.0
100 x 2.1
Price
7 m Columns (mm)
Part No.
Mfr. No.
Description
CH0-3305
35007-104630 Hypercarb
CH0-3309
35007-054630 Hypercarb PCB
Size (mm)
100 x 4.6
50 x 4.6
Price
* Hypercarb is a trademark of Thermo Hypersil-Keystone.

Phenomenex is not associated with Thermo Fisher Scientific or Thermo Hypersil-Keystone.
For a complete list of applications contact your

For additional chiral separations, see Lux columns on

pp. 318-323.
Phenomenex
317
Lux
If Lux analytical columns ( 4.6 mm ID)

do not provide at least an equivalent
or better separation as compared to a
competing column of the same particle
size, similar phase and dimensions,
Five Distinct Polysaccharide

Phases to Resolve 92 % of
Your Enantiomers*
Lux columns offer a wide and complementary range of enantioselectivity for even the most difficult chiral separation projects.
SIMPLIFIED CHIRAL SEPARATIONS
High efficiency and loading capacity

Stable in normal phase, polar organic, SFC, and reversed
phase conditions
3 m and 5 m packed columns and 20 m bulk media for
scale up
C-2
C-1
Technical Specifications
Particle Size
pH Stability
3, 5, 20 m
2-9
Maximum Pressure
Temperature Range
Shipping Solvent
300 bar
0-50 C
n-Hexane/2-propanol (9:1, v/v)
H
H
C
H
Please inquire for availability
Cellulose-O-CONH
chiral lc
C-1
lux
Lux chiral columns are made with high quality silica, and undergo a
specialized coating and packing process that ensures high efficiencies while maintaining essential enantiorecognition abilities.
C-1
H
H
C-3
C-3
Cellulose tris(3,5-dimethylphenylcarbamate)
H
H
H
H
Lux Cellulose-1
C-2
C-2
Cellulose-O-CONH
H
Cl
Cl
Cl
Cl
C-4
A-2
C-4
Cellulose-O-CONH
Cellulose-O-CONH
Cellulose-O-CONH
SFC
APPROVED
H
H
C
C
Cellulose-O-CONH
Cellulose-O
Cellulose-O
Cellulose-O-CONH
Lux Cellulose-2
Lux Cellulose-3
Cellulose tris(3-chloro-4-methylphenylcarbamate)
Cellulose tris(4-methylbenzoate)
C-2
C-3
H
H
C-3
C-4
A-2
C-4
A-2
H
H
H
H
Cl
C
Cl
Cl
Cl
Cl
Cellulose-O-CONH
Cellulose-O
Cellulose-O
Cellulose-O-CONH
C
H
Cellulose-O-CONH
Amylose
-O-CONH
Amylose -O-CONH
Lux Cellulose-4
Lux Amylose-2
Amylose tris(5-chloro-2-methylphenylcarbamate)
* Based on 233 compounds screened on all five Lux phases
318
Cellulose-O-CONH
Amy los e-O-CONH
Phenomenex
Lux
Achieve Optimal Resolution by Screening all Five

Complementary Lux Chiral Columns
Utilizing differences in selectivity can help develop methods more
efficiently by offering broad and contrasting chiral recognition abilities. Lux chiral selectors provide a variety of selectivities that give
you the opportunity to screen for the ideal chiral separation.
Rabeprazole
Lux 5 m Amylose-2
19733
App ID 19733
a = 0.00
0
19729
10
12

Mobile Phase: 0.1 % Diethylamine in 10 mM Ammonium bicarbonate/
0.1 % Diethylamine in Methanol
(80:20)
14 min
H
N
Lux 5 m Cellulose-1
O
S
App ID 19729
a = 1.30
19730
0
10
12
14
N
O
O
min
lux
Lux 5 m Cellulose-2
a = 1.19
App ID 19730
chiral lc
19731
0
10
12
14
min
Lux 5 m Cellulose-3
a = 0.00
SFC
App ID 19731
APPROVED
10
12
14
min
19732
Optimal Resolution
Based on a five phase screen under reversed phase

conditions, the optimal chiral stationary phase for
resolving Rabeprazole is Lux Cellulose-4.
Lux 5 m Cellulose-4
App ID 19732
a = 1.53
10
12
14
min
Phenomenex
319
Lux
C-2
Lux Chiral Stationary Phases

C-3
C-4
A-2
H
H
The Lux line of cellulose and amylose-based

chiral stationary
H
H
C
H
phases
includes five complementary selectivities.
Cl
H
Lux Amylose-2:
Chlorinated Amylose Chiral Selector
Cl
Cl
C
H
O
This first-to-market chlorinated amylose
phenylcarbamate derivative offers complex chiral recognition components that greatly
Cellulose-O-CONH
increase the chances of achievingCellulose-O
chiral resolution.
Cellulose-O-CONH
Amylose -O-CONH
2-Phenylcyclohexanone
Lux 5 m Amylose-2
18098
mAU
*CHIRALPAK 5 m AD-H
18097
mAU
a = 1.24
100
1 2
a = 1.04
100
App ID 18097
App ID 18098

Mobile Phase: 0.1 % Diethylamine in Hexane /
0.1 % Diethylamine in Isopropanol
(90:10)
Flow Rate: 1 mL/min
0
0
10
12
min
10
12
min
C-2
lux
C-1
chiral lc
Lux Cellulose-1:
Dimethyl Cellulose Chiral Selector
This universally trusted cellulose phenylcarbamate derivative is absolutely

essential to any chiral screen. Guaranteed alternative to Chiralcel OD-H.
Expect equivalent or better preformance.
Cellulose-O-CONH
Cellulose-O-
Metoprolol
mAU
mAU
200
200
a = 1.38
0
0
*CHIRALCEL 5m OD-H
17423
App ID 17423
APPROVED
Lux 5m Cellulose-1
17422
App ID 17422
SFC
a = 1.16

Mobile Phase: 0.1 % Diethylamine in Acetonitrile /
(95:5)
Flow Rate: 1 mL/min
0
0
min
OH
O
H
N
CH3
CH3
H3CO
320
Phenomenex
Lux
C-2
C-1
Lux Cellulose-2:
Chlorinated Cellulose Carbamate Phase
Cl
H
Cellulose-O-CONH
Cellulose-O-CONH
Cellulos
Tolperisone
Lux 5 m Cellulose-2
17323
*CHIRALCEL 5 m OD-H
17322
mAU
600
App ID 17323
1
400
a = 1.69
App ID 17322
mAU
600
400
a = 1.07
200
0
0
0
This first-to-market halogenated cellulose phenylcarbamate derivative offers unique chiral recognition abilities that complement
the rest of the Lux family of columns.
200
C-3
min

(90:10)
Flow Rate: 1 mL/min
min
O
N
CH 3
H 3C
C-2
C-1
C-3
H
H
Cl
Cellulose-O-CONH
chiral lc
=1.17
Cellulose-O-CONH
Cellulose-O
Disopyramide
Lux 5 m Cellulose-3
*CHIRALCEL 5 m OJ-H
a = 1.17
Rs = 2.03
App ID 19157
App ID 19156

0.1 % Diethylamine in Ethanol
(90:10)
Flow Rate: 1 mL/min
N
4
min
Rs=2.03
a = 1.18
Rs = 2.30
H
This cellulose methylbenzoate
derivative offers distinct and
Rs=2.30
complementary
chiral
recognition
abilities.
19157
=1.18
19156
H
H
lux
Lux Cellulose-3:
Cellulose Ester Phase
Cellulose
SFC
APPROVED
min
NH2
Phenomenex
321
Lux
C-2
C-1
C-3
C-4
A-2
H
H
H
H
Lux Cellulose-4:
Chlorinated Cellulose Carbamate Phase
H
Cl
Cl
This chlorinated cellulose phenylcarbamate derivative offers unique

chiral recognition abilities.
Cellulose-O-CONH
19153
Cellulose-O-CONH
Cellulose-O
Cellulose-O-CONH
= 1.17
Lux 5 m Cellulose-4
= 1.00
*CHIRALPAK 5 m AD-H

(90:10)
Flow Rate: 1 mL/min
a = 1.17
min
App ID 19155
App ID 19153
a = 1.00
0
min
N
H 3C
CH 3
chiral lc
lux
Chiral Screening in HPLC and SFC

Have a sample and need a separation method? We can help!
Reliable methods and fast turnarounds are our specialty. Depending on compound type, we
screen multiple chiral stationary phases under different conditions and return a detailed report of
the recommended separation method so you can begin work right away.
We provide the following services:

SFC
APPROVED
Chiral Screening
Method Optimization Services
Preparative and Process Scale-Up
Normal Phase
Fast Turnaround
Media Screening
Reversed Phase
Easy Method Transfer
Small Scale Purification
Polar Organic
Continued Support
DAC Packing Assistance
SFC
For more information or to begin a project today,

please contact your local Phenomenex representative
or email us at: phenologix@phenomenex.com
You can also visit us online: www.phenomenex.com/phenologix
322
Amy los e-
19155
Tolperisone
Phenomenex
Lux
If Lux analytical columns ( 4.6 mm ID)

do not provide at least an equivalent
or better separation as compared to a
size, similar phase and dimensions,
3m Analytical Columns (mm)
Phases
50 x 2.0
150 x 2.0
50 x 4.6
100 x 4.6
150 x 4.6
250 x 4.6
Cellulose-1
Cellulose-2
Cellulose-3
Cellulose-4
00B-4458-B0
00B-4456-B0
00B-4492-B0
00B-4490-B0
00F-4458-B0
00F-4456-B0
00F-4492-B0
00F-4490-B0
00B-4458-E0
00B-4456-E0
00B-4492-E0
00B-4490-E0
00D-4458-E0
00D-4456-E0
00D-4492-E0
00D-4490-E0
00F-4458-E0
00F-4456-E0
00F-4492-E0
00F-4490-E0
00G-4458-E0
00G-4456-E0
00G-4492-E0
00G-4490-E0
Amylose-2
00B-4471-B0
00F-4471-B0
00B-4471-E0
00D-4471-E0
00F-4471-E0
00G-4471-E0
for ID:
5m Analytical Columns (mm)

Phases
50 x 2.0
Cellulose-1
Cellulose-2
Cellulose-3
Cellulose-4
Amylose-2
00B-4459-B0
00B-4457-B0
00B-4493-B0
00B-4491-B0
00B-4472-B0
50 x 4.6
100 x 4.6
150 x 4.6
00B-4459-E0
00B-4457-E0
00B-4493-E0
00B-4491-E0
00B-4472-E0
00D-4459-E0
00D-4457-E0
00D-4493-E0
00D-4491-E0
00D-4472-E0
00F-4459-E0
00F-4457-E0
00F-4493-E0
00F-4491-E0
00F-4472-E0
SecurityGuard
Cartridges (mm)
5m Semi-Prep Columns (mm)
00G-4459-N0
00G-4457-N0
00G-4493-N0
00G-4491-N0
00G-4472-N0
for ID:
5m Axia Packed Preparative Columns (mm)

Phases
150 x 21.2
250 x 21.2
aufInquire
Anfrage
aufInquire
Anfrage
Cellulose-1
00F-4459-P0-AX
00G-4459-P0-AX
Cellulose-2
00F-4457-P0-AX
00G-4457-P0-AX
Cellulose-3
00F-4493-P0-AX
00G-4493-P0-AX
Cellulose-4
00F-4491-P0-AX
00G-4491-P0-AX
Amylose-2
00F-4472-P0-AX
00G-4472-P0-AX
10 x 10.0
/3pk
AJ0-8404
AJ0-8399
AJ0-8623
AJ0-8628
AJ0-8472
9 16 mm
250 x 30
aufInquire
Anfrage
00G-4459-U0-AX
00G-4457-U0-AX
00G-4493-U0-AX
00G-4491-U0-AX
00G-4472-U0-AX
*SecurityGuard Analytical Cartridges require holder, Part No. : KJ0-4282

20m Bulk Media

Phases
Cellulose-1
Cellulose-2
Cellulose-3
Cellulose-4
Screening Kit
AJ0-8470
3.28.0 mm

4 x 2.0*
4 x 3.0*
/10pk
/10pk
AJ0-8402
AJ0-8403
AJ0-8398
AJ0-8366
AJ0-8621
AJ0-8622
AJ0-8626
AJ0-8627
AJ0-8471
AJ0-8470
2.0 3.0 mm
3.28.0 mm
1 kg
aufInquire
Anfrage
04K-4473
04K-4464
04K-4504
04K-4503
Please inquire for 20m Lux Amylose-2 media.
250 x 50
aufInquire
Anfrage
00G-4459-V0-AX
00G-4457-V0-AX
00G-4493-V0-AX
00G-4491-V0-AX
00G-4472-V0-AX
for ID:

15 x 21.2**
15 x 30.0+
/ea
/ea
AJ0-8405
AJ0-8406
AJ0-8400
AJ0-8401
AJ0-8624
AJ0-8625
AJ0-8629
AJ0-8630
AJ0-8473
AJ0-8474
1829 mm
3049 mm
SFC
APPROVED
**PREP SecurityGuard Cartridges require holder, Part No. : AJ0-8223
PREP SecurityGuard Cartridges require holder, Part No. : AJ0-8277
3m Method Screening Kits

(save 10 % when purchased as a kit)
50 x 4.6 mm
aufInquire
Anfrage
KH0-8483
100 g
aufInquire
Anfrage
04G-4473
04G-4464
04G-4504
04G-4503
AJ0-8471
2.03.0 mm
chiral lc
00F-4459-N0
00F-4457-N0
00F-4493-N0
00F-4491-N0
00F-4472-N0
250 x 10.0
AJ0-8403
AJ0-8366
AJ0-8622
AJ0-8627
Cellulose-1
Cellulose-2
Cellulose-3
Cellulose-4
Amylose-2
150 x 10.0
00G-4459-E0
00G-4457-E0
00G-4493-E0
00G-4491-E0
00G-4472-E0
for ID:
AJ0-8402
AJ0-8398
AJ0-8621
AJ0-8626
lux
Phases
250 x 4.6

4 x 2.0*
4 x 3.0*
/10pk
/10pk
250 x 4.6 mm
aufInquire
Anfrage
KH0-8482
Column Performance Check Standard

Part No.
AL0-8412
Description
Chiral Test Mix No. 5 (Lux)
Unit
ea
Price
Kit contains one column of each phase.
*CHIRALPAK, CHIRALCEL, AD-H, OD-H, and OJ-H are registered trademarks of DAICEL Chemical Industries, Ltd.
Columns used for comparison were manufactured by DAICEL Chemical Industries, Ltd.
Phenomenex
323

Includes bonded cyclodextrins (ChiraDex) and Whelk-O phases from Regis.
Chiral Columns
Part No.
Mfr. No.
Description
Particle Size (m)
Stainless Steel LiChroCART ChiraDex/Whelk-O 1
CH0-5443
1.51333.0001*
ChiraDex ()
5
CH0-5444
1.50117.0001*
ChiraDex ()
5
CH0-7906**
(R,R) Whelk-0 1, 100
5
CH0-7905**
(S,S) Whelk-0 1, 100
5
Size (mm)
Unit
250 x 4
4x4
250 x 4.6
250 x 4.6
ea
10/pk
ea
ea
Price
*ChiraDex stainless steel LiChroCART cartridges supplied in methanol/water (40:60);

also recommended for storage.
Cartridges require manu-CART fittings: CH0-5286.
**Whelk-O columns supplied in n-Hexane.
Nucleosil Chiral
The purity and narrow particle and pore size distributions of

Nucleosil silicas provide the base materials for some of the highest quality HPLC chiral stationary phases. The variety of chemistries are designed to promote chiral separations through diverse
mechanisms directly, quickly and without derivatization (see table
Stationary Phase Type
Chemistry
Mechanism
Applications
Chiral 1
ligand exchange
L-hydroxyproline-Cu++
N-methyl--amino acids, -alkyl-amino acids, -amino alcohols
diastereomeric metal complex
-amino acids, -hydroxy acids,
Chiral 2
brush (Pirkle)
D-dinitro-benzoyl
phenylglycine
Chiral 3
Nucleodex -OH
Nucleodex -PM
phenylglycine
L-dinitro-benzoyl
cavity
native -cyclodextrin
permethylated--cyclodextrin
inclusion complexes
carboxylic acids, and esters, amides,

imides, geometric and positional isomers
Resolvosil BSA-7
protein
bovine serum albumin
hydrophobic interactions
polar interactions steric effects
wide variety of low MW

pharmacologically active compounds
attractive interactions
hydrogen bonding
charge transfer (- interaction)
dipole stacking
organic-soluble pharmaceuticals
and agrochemicals
Ethynylestradiol
Oxazepam
Column:
Dimensions:
Part No.:
Mobile Phase:
Column:
Dimensions:
Part No.:
Mobile Phase:
Nucleodex -PM
200 x 4.0 mm
CH0-3027
Methanol/0.1 % TEAA
pH 4.0 (70:30)
Resolvosil BSA-7
150 x 4.0 mm
CH0-1998
1 % 1-Propanol in
0.1 M Phosphate buffer, pH 6.8
4
Nucleosil Chiral Columns
Part No.
Mfr. No.
CH0-1994
720081.40
CH0-7047
721455.40
CH0-1996
720088.40
CH0-1997
720350.40
CH0-3025
720124.40
CH0-7048
721460.40
CH0-3027
720125.40
CH0-7049
721462.40
CH0-1998
720046.40
CH0-5812
721359
324
12
App ID 5425
App ID 5424
chiral lc
Merck / nucleosil chiral
Column
below). Macherey-Nagels chiral stationary phases are highly suited

for resolving a wide range of enantiomeric pairs, including many
important pharmaceutical, pesticide and biochemical compounds.
Phenomenex offers the complete line of Macherey-Nagel chiral
columns.
min
Column Description
Nucleosil Chiral-1 L-hydroxyproline-Cu++ complex
Nucleosil Chiral-1 guard column (requires adapter)
Nucleosil Chiral-2 D-dinitrobenzoyl phenylglycine, Pirkle-type
Nucleosil Chiral-3 L-dinitrobenzoyl phenylglycine, Pirkle-type
Nucleodex -OH Cyclodextrin
Nucleodex -OH guard column (requires adapter)
Nucleodex -PM Cyclodextrin
Nucleodex -PM guard column (requires adapter)
Resolvosil BSA-7 300 pore, Protein-based
Guard column adapter, 8 mm
Size (mm)
250 x 4.0
8 x 4.0
250 x 4.0
250 x 4.0
200 x 4.0
8 x 4.0
200 x 4.0
8 x 4.0
150 x 4.0
10 min
Price
Phenomenex
Shodex Chiral
By Showa Denko K.K.

Wide range of applications
Basic Structure of CD Bonded Phase
Durable polymer matrix enables wide pH stability

Chemically modified cyclodextrins improve
enantioselectivity
Cyclodextrin phases for the direct separation of enantiomers have
become increasingly popular due to their wide applicability. Showa
Denko has taken these useful phases a few steps further by modifying
the cyclodextrins with carboxymethyl (CM) functionality and bonding
them to a chemically and physically stable polymer backbone.
Modification of the cyclodextrins improves enantioselectivity for
many compounds compared to native cyclodextrins. Utilization of
durable polymethacrylate polymer as the anchor for this stationary
phase enables use of highly alkaline and acidic mobile phases, not
possible with silica-based CD phases. Separation of enantiomers of
amino acids and their derivatives, amino alcohols, carboxylic acids,
amines, alcohols and others are routinely performed with these
columns.
Comparison of Retention Behavior Between

Modified and Unmodified CD Bonded Phases
Bonded Phase
Met - NA*
Rs
CD
2.0
CM-CD
2.2
* Met-NA: Methionine 2-naphthylamide
** POPA: 2-Phenoxypropionic acid
2.4
3.3
POPA**
Rs
1.1
1.2
0.5
1.4
ID x Length (mm)
4.6 x 150
Ligand
-Cyclodextrin
derivatives
ORpak CDC-453HQ
4.6 x 150
-Cyclodextrin
derivatives
Price
Shodex Chiral Columns

ORpak CDB-453HQ
Shodex / ULTron es
chiral lc
For In-line Filters specifically designed to protect your chiral

column investment, see p. 317
Ultron ES
Manufactured by Shinwa Chemical Industries, Ltd.

Two complementary protein-based chiral
stationary phases
Easy to use with reversed-phase mobile phases
Racemic separation without derivatization
pH range from 3.0 to 7.5
Ultron ES Chiral Columns
Column
m
Analytical
5
Analytical & Guard
5
Phenomenex
Size (mm)
150 x 4.6
150 x 4.6
ES-OVM
702111651
702111651A
Price
ES-Pepsin
822111651
822111631A
Price
325
Sumichiral OA
By Sumika Chemical Analysis Service, Ltd.

High chromatographic efficiency
Rapid recovery from overload
Wide range of applications in both normal
and reversed phase*
Crown Ether column available
Sumichiral OA columns, include Pirkle-type, cavity type
(cyclodextrin and crown ether), as well as ligand-exchange-type
columns for the separation of enantiomers by HPLC. A wide
diversity of optically active compounds of biological importance
can be resolved directly with these columns, including many
pharmaceuticals, biochemicals and pesticides. A variety of chiral
selectors bonded to 5 m silica gives you a choice of selectivity
for virtually all your chiral applications. If you have an interest in
any Sumichiral OA column not listed here, please call one of our
technical representatives for more information.
chiral lc
Sumichiral OA Chiral
Sumichiral OA Chiral Columns (mm)
Phases
Description
Type
OA-2000
OA-3100
OA-3200
OA-3300
OA-4000
OA-4100
OA-4400
OA-4500
OA-4600
OA-4700
OA-4800
OA-4900
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
Covalent
(R)-phenylglycine and 3,5-dinitrobenzoic acid

(S)-valine and 3,5-dinitroanaline
(S)-tert-leucine and 3,5-dinitroanaline
(R)-phenylglycine and 3,5-dinitroanaline
(S)-valine and (S)-1-(-naphthyl)-ethylamine
(S)-valine and (R)-1-(-naphthyl)-ethylamine
(S)-proline and (S)-1-(-naphthyl)-ethylamine
(S)-proline and (R)-1-(-naphthyl)-ethylamine
(S)-tert-leucine and (S)-1-(-naphthyl)-ethylamine
(S)-tert-leucine and (R)-1-(-naphthyl)-ethylamine
(S)-indoline-2-carboxylic acid and (S)-1-(-naphthyl)ethylamine
(S)-indoline-2-carboxylic acid and (R)-1-(-naphthyl)ethylamine
OA-2500 (R)-1-naphthylglycine and 3,5-dinitrobenzoic acid

OA-2500-I (R)-1-naphthylglycine and 3,5-dinitrobenzoic acid
OA-5000
OA-5500
OA-6100
OA-7000
(D)-penicillamine
chiral Schiff base
(L)-tartaric acid-mono-(L)-valine-(S)-1-(-naphthyl)ethylamide
-Cyclodextrin (derivatized)
OA-8000
Crown ether
Covalent
Ionic
150 x 4.6
Ligand Exchange
Ligand Exchange
Ligand Exchange
Cavity
CH0-1859
CH0-1861
CH0-1863
150 x 4.6
Cavity
CH0-7050
250 x 4.6
CH0-1752
CH0-1770
CH0-1776
CH0-1782
CH0-1788
CH0-1794
CH0-1800
CH0-1806
CH0-1812
CH0-1818
CH0-1824
CH0-1830
250 x 4.6
CH0-1836
CH0-1848
250 x 4.6
CH0-5649
*For a list of applications, please contact your Phenomenex representative
Protect your valuable column investment with the disposable

KrudKatcher pre-column filter, see p. 17
For In-line Filters specifically designed to protect your chiral column
investment, see p. 317
For Chiral Column Performance Check Standards, see p. 366
326
Phenomenex
Supercritical Fluid
Chromatography (SFC)
supercritical fluid
chromatography (SFC)
SFC Media
Chiral: Lux SFC Media........................................................................... 329

Achiral: Kinetex, Luna and Synergi SFC Media..................................... 331
Preparative Media.................................................................................. 333
295
327-333
- 302
Supercritical Fluid Chromatography (SFC)

Supercritical Fluid
Chromatography (SFC)
SFC is recognized by scientists worldwide as a clean, green, and
efficient tool for analysis and purification. With recent advancements and accessibility of instrumentation, improved column hardware, and the wide variety of surface chemistries available, SFC
has enjoyed an ever-increasing range of applications in many industries:
Pharmaceutical
Nutraceutical
Petrochemical
Natural Products
Food & Beverage
Environmental
SFC
APPROVED
Expanding the Range of

Selectivity for SFC
Selecting a column is one of the most critical parameters during
SFC method development. Having a variety of complementary and
orthogonal selectivities to choose from can mean the difference
between partial or no separation and achieving an optimal
separation that can be validated and scaled-up in your lab.
Academic
and more
Phenomenex offers a large collection of packed SFC analytical and

preparative columns that have earned their reputations for reliability,
high efficiency, reproducibility, and long lifetimes.
Supercritical Fluid Chromatography | SFC
Complete SFC Product Offering

Phenomenex offers solutions for your SFC needs.
Over 20 selectivities for use in SFC
Chiral and achiral phases available
Multiple particle sizes ranging from 1.7 m thru 20 m*
Scalable packed column dimensions (2.0 mm 50.0 mm ID)
Chiral columns (pp. 329-330)
Achiral columns (pp. 331-332)
*Not all media available in a full range of particle sizes, please inquire.
SFC
APPROVED
328
Phenomenex

Chiral SFC Media
Exceptional Stability and Separating Power under SFC Conditions
Multiple complementary polysaccharide stationary phases
High efficiency and loading capacity
Pressure stability up to 300 bar
3 m, 5 m packed columns and 20 m bulk media for scale up
Lux Amylose-2
Lux Cellulose-3
Halogenated amylose chiral selector for superior

enantiorecognition
A-2
C-1
Cellulose ester derivative for broad range of

enantioselectivity
C-2
C-3
C-4
H
H
H
H
Cl
Cl
Cl
A m y l o s e-O-CONH
Cellulose-O-CONH
Cellulose-O-CONH
Cellulose-O
Cellulose-O-CONH
Lux Cellulose-1
Lux Cellulose-4
Guaranteed replacement for *CHIRALCEL OD-H

at a sensible price
C-2
C-1
H
H
H
H
C
H
C-4 C-3
Halogenated chiral selector for enhanced

recognition and complementary selectivity
C-3 C-2
C-4
H Cl C
H
H
Cl
Cl
CCl
A-2
SFC | Supercritical Fluid Chromatography
Cl
Cellulose-O-CONH
Cellulose-O-CONH
Cellulose-O
Cellulose-O-CONH
Cellulose-O-CONH
Cellulose-O
Amylose
-O-CONH
Cellulose-O-CONH
Lux Cellulose-2
C-2
H
H
SFC
Chlorinated CSP provides unique

chiral separation abilities
C-1
C-3
C-4
H
H
Cl
APPROVED
Cl
Cellulose-O-CONH
Cellulose-O-CONH
Cellulose-O
Cellulose-O-CONH
* CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd.
Phenomenex
329

Extreme Stability and Separating Power under SFC Conditions
Never fear crushed media or loss in efficiency again. With a pressure stability up to 300 bar (4350psi), you
can feel confident about running at high operating pressures (if necessary). Lux media is SFC approved and
versatile enough to satisfy all of your chiral separation needs.
Bifonazole
18855

1
2
0
19098
App ID 18855
Lux 5m Cellulose-1
a = 1.08
10
12
14
16
18
20
22

Mobile Phase: 0.1% Diethylamine in Methanol / Carbon Dioxide
(15:85)
Temperature: 35C
Detection: Diode Array Detector
24 min
N
2
10
12
14
16
18
20
22
24 min
Smaller Particles for Higher Efficiency

Scaling down to a 3 m particle gives you exceptional efficiencies and significantly reduced runtimes without
compromising enantioselectivity.
18107
Bendroflumethiazide
mAU
Lux 5 m Cellulose-2
200
App ID 18207
App ID 19098
*CHIRALCEL 5m OD-H
a = 1.02
0
18206
SFC
APPROVED
8
1
mAU
Column:
Dimensions:
Part No.:
Mobile Phase:
300
App ID 18208
Optimized for speed
200
Lux 5 m Cellulose-2
250 x 4.6 mm
00G-4457-E0
0.1 % Diethylamine with 0.1 % Trifluoroacetic acid in
Methanol / Carbon Dioxide (30:70)
Flow Rate: 2 mL/min
min
Lux 3 m Cellulose-2
400
Column:
Dimensions:
Part No.:
Mobile Phase:
100
Lux 3 m Cellulose-2
50 x 4.6 mm
00B-4456-E0
0.1 % Diethylamine with 0.1 % Trifluoroacetic acid in
Methanol / Carbon Dioxide (30:70)
Flow Rate: 4 mL/min
0
0
0.1
0.2
0.3
0.4
0.5
min
*CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd. Comparative separations may not be representative of all applications.
330
Phenomenex

Achiral SFC Media
Core-shell* and fully porous media
High surface area for increased loading
Easy scale-up from lab to pilot plant
Unique polar and non-polar selectivities for screening
Columns interchangeable between SFC and HPLC modes
Synergi Polar-RP
O
O
OH
O
O
OH
O
OH
OH
OH
OH
OH
Luna HILIC
OH
O-
Aq
Cross-linked Diol
Kinetex PFP
F
O
Si
Aq
Ether-Linked Phenyl
Luna Si(2)
Si
Kinetex HILIC
HO
OH
Si
Aq Si Aq Aq
Aq
Si
HO
F
F
F
HO
F
Si
Si
HO
Si
Aq
i Aq
Aq S
OH
F
F
Porous
Core-Shell
Porous
Pentafluorophenyl (PFP)
Core-Shell
Silica, Unbonded
Luna PFP(2)
Luna CN
Luna NH2
NH2
NH2
NH2
NH2
CN CH2
CN CH2
Si
Si
Si
Amino
CN CH2
Si
Si
Si
SFC
Si
APPROVED
Cyano
Additional Non-Polar Phases Available

C18/C8/C4
Phenyl-Hexyl
TWIN Technology C18
Fusion-RP
TWIN Technology C6-Phenyl
Hydro-RP and more
Phenomenex
*For more information on core-shell Kinetex media,

please see p. 180
**Bulk media available, please inquire
331

Media Selectivity is Critical for Success
Utilizing unique differences in surface chemistries will ensure that you achieve a successful
separation for any given project, as in the example below. Once the ideal column phase is
identified, you have the ability to optimize for additional improvements in performance:
Changing retention
Altering selectivity
Increasing efficiency
Reversing elution orders
These optimization steps can easily be achieved by adjusting a few simple parameters. For
instance, you can try different modifiers and/or additives, change the percent concentration of
your modifier, or you can simply change your pressure, temperature, and/or flow rate.
Unique Selectivity
More Possibilities
Optimization
Complementary Phases Offer Reversed Elution Order!

Stationary Phase Effects on Selectivity
Synergi 4 m Polar-RP 80
16625
Luna 5 m HILIC (Cross-linked Diol) 200
16623
1,2
min
Luna 5 m NH2 100
16624
App ID 16623
App ID 16625
min
Luna 5 m Silica(2) 100
16622
APPROVED
min
App ID 16622
App ID 16624
1
2
SFC
2
1
min
Conditions same for all columns:

Mobile Phase: A: Methanol
B: Carbon Dioxide (CO2)
Gradient: A/B (5:95) to (50:50) in 5.5 min, hold 2 min
Temperature: 40 C
Pressure: 2900 psi (200 bar)
Sample: 1. Uracil
2. Hydrocortisone
3. Sulfanilamide
4. Ethyl-2-methyl-3-indoleacetate
The comparative data presented here may not be representative for all applications.
332
Phenomenex

Achiral Material Characteristics
Packing Material
Porous
Luna Silica(2)
Luna HILIC (cross-linked diol)
Luna PFP(2)
Luna CN
Luna NH2
Synergi Polar-RP
Particle
Size
(m)
Pore
Size
()
Surface
Area
(m2/g)
Carbon
Load
%
End
Capping
pH
Stability
3, 5,10,15
3, 5
3, 5
3, 5, 10
3, 5, 10
2.5, 4, 10
100
200
100
100
100
80
400
200
400
400
400
475
0
5.7
11.5
7.0
9.5
11
No
No
Yes
Yes
No
proprietary
2.0 - 7.5
1.5 - 8.0
1.5 - 8.0
1.5 - 7.0
1.5 - 11.0
1.5 - 7.0
1.7, 2.6
1.7, 2.6
100
100
200
200
0
9
Yes
Yes
2.0 - 7.5
1.5 - 8.5
Packing Material
Core-Shell
Kinetex HILIC
Kinetex PFP
Chiral Material Characteristics

Packing Material
Porous
Particle
Size
(m)
Pressure
Stability
(bar)
pH
Stability
Lux Cellulose
Lux Amylose
3, 5, 20*
3, 5, 20*
300
300
2.0 - 9.0
2.0 - 9.0
* Please inquire
Many non-polar phases available as well!
250 x 4.6 mm
aufInquire
Anfrage
00G-4274-E0
00G-4450-E0
00G-4448-E0
00G-4255-E0
00G-4378-E0
00G-4336-E0
250 x 10 mm
aufInquire
Anfrage
00G-4274-N0
00G-4450-N0
00G-4448-N0
00G-4255-N0
00G-4378-N0
00G-4336-N0
250 x 21.2 mm
aufInquire
Anfrage
00G-4274-P0-AX
00G-4450-P0-AX
00G-4448-P0-AX
00G-4255-P0-AX
00G-4378-P0-AX
00G-4336-P0-AX
250 x 30 mm
aufInquire
Anfrage
00G-4274-U0-AX
00G-4450-U0-AX
00G-4448-U0-AX
00G-4255-U0-AX
00G-4378-U0-AX
00G-4336-U0-AX
250 x 50 mm
aufInquire
Anfrage
00G-4274-V0-AX
00G-4450-V0-AX
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
250 x 10 mm
250 x 21.2 mm
aufInquire
Anfrage
00G-4459-P0-AX
00G-4457-P0-AX
00G-4493-P0-AX
00G-4491-P0-AX
00G-4472-P0-AX
250 x 30 mm
aufInquire
Anfrage
00G-4459-U0-AX
00G-4457-U0-AX
00G-4493-U0-AX
00G-4491-U0-AX
00G-4472-U0-AX
250 x 50 mm
aufInquire
Anfrage
00G-4459-V0-AX
00G-4457-V0-AX
00G-4493-V0-AX
00G-4491-V0-AX
00G-4472-V0-AX
*Available in 3 m.
Phase
Core-Shell Kinetex Technology
Kinetex 2.6 m HILIC
Kinetex 2.6 m PFP
100 x 4.6 mm*
150 x 4.6 mm
00D-4461-E0
00D-4477-E0
00F-4461-E0
00F-4477-E0
150 x 4.6 mm*
250 x 4.6 mm
Supercritical Fluid Chromatography (SFC) Columns
Phase
150 x 4.6 mm*
Achiral Columns
aufInquire
Anfrage
Luna 5 m Silica
00F-4274-E0
Luna 5 m HILIC (cross-linked diol)
00F-4450-E0
Luna 5 m PFP
00F-4448-E0
Luna 5 m CN
00F-4255-E0
Luna 5 m NH2
00F-4378-E0
00F-4336-E0
*Available in 3 m .
Phase
Chiral Columns
Lux 5 m Cellulose-1
Lux 5 m Cellulose-2
Lux 5 m Cellulose-3
Lux 5 m Cellulose-4
Lux 5 m Amylose-2
00F-4459-E0
00F-4457-E0
00F-4493-E0
00F-4491-E0
00F-4472-E0
00G-4459-E0
00G-4457-E0
00G-4493-E0
00G-4491-E0
00G-4472-E0
00G-4459-N0
00G-4457-N0
00G-4493-N0
00G-4491-N0
00G-4472-N0
SFC
APPROVED
*Available in 3 m. Additional phases and dimensions available upon request.
Bulk SFC media is available. Please contact your Phenomenex

representative for more information.
For SFC PREP SecurityGuard Cartridge Holders

for 21.2 and 30 mm ID, use Part Nos. AJ0-8617
and AJ0-8618, respectively.
For all other SecurityGuard Cartridge Holders and
Phenomenex
333
Ingenuity, Im
a
gin
30
atio
ng
brati 30 Yea
e
l
e
r
f
so
n, and H
ea
r
We played.
Its true what youve heard. We really do look for every excuse to play! What other
separations company gives their sales teams cooking lessons, holds their own
annual PhenOlympics games, or runs around office hallways in giant HPLC particle
costumes for fun? Between the hallway bowling tournaments, and the practical
jokes that would make you go bonkers in delight, you might wonder who our biggest instigators are. Well, dont be surprised to learn
that its the senior management themselves! Perhaps
this is how we earned our reputation for being a bit
mad. A bit silly. But a LOT different. And we love it.
We raced.
Thousands of kilometers for good causes, around tracks in go-carts, over river
rapids, and down steep snowy mountains. We race until our feet are sore and our
cheeks hurt from smiling. And thats only a taste of our adventures. Deep sea fishing, camping, canoeing, sailing, cycling,
triathalons, mud runs there is so much
fun to be had, and we try to do it all. Work
hard, play hard. Its in our blood.
Can we ask you something? See page 408.

334
Phenomenex
Prep Columns
and
Bulk Media
Prep Columns
and Bulk Media
Axia Packed Preparative HPLC Columns........................................... 336-343
Process Chromatography................................................................... 344-352

Bulk Media...................................................................................... 344-352
Columns, Scout and Preparative.................................................... 347-350
Sepra Bulk Sorbents.............................................................................. 352
335 - 352
Axia Packed Preparative HPLC
If Axia packed columns do not provide

LONGER LIFETIME when used with
SecurityGuard PREP, as compared to a
size, phase and dimensions, send in your
comparative data and the column within
U.S. Patent No. 7, 674, 383
Axia
Preparative Chromatography Redefined

Axia patented technology is an advanced column packing and
hardware design that eliminates media bed collapse as a source
of premature failure in chiral and achiral preparative columns.
Axia Hydraulic Piston Compression
First ever integrated packing piston in a column allows:

The piston compresses the media bed and pressure
is never released (no chance of media loss or
recompression)
The optimum slurry amount and packing pressure are
automated for reproducible packing control
Uniform packing density and consistent peak shape
every time
A complete departure from conventional slurry packing
methods means:
Longer lifetime with patented technology
View the packing process @ www.phenomenex.com/info/Axia
Improved column-to-column reproducibility

Recover higher compound purity with analytical-like
efficiency
PREP COLUMNS & bulk media
AXIA
Packing Piston
The packing piston head is

integrated into the column
and locked by the piston
retainers, so the pressure is
never released.
Media packed under

ideal pressure
Media is never allowed

to relax, eliminating
voids and dramatically
improving reproducibility,
column-to-column.
SEM of Inlet Frits After Packing

Axia Packed Media
Traditional Packed Media
SFC
APPROVED
Media is largely intact at frit

surface after packing
336
Inlet frit shows crushed media

particles or fines at frit surface
after packing
Phenomenex
U.S. Patent No. 7, 674, 383
Expect Better Performance.

Expect an Excellent Axia Column.
Every Time.
2006 R&D 100

Award Recipient
Consistent Quality. Column-to-Column. Batch-to-Batch

Reproducible
Column-to-Column Efficiency
Average Efficiency (N) with Synergi
4 m Hydro-RP 100 x 21.2 mm
Average Peak Asymmetry with Gemini

5 m C18 50 x 21.2 mm
62
120,000
% RSD
2.5
% RSD
61
1.4
100,000
9.2
1.3
80,000
60,000
13 % Improved Avg.
Peak Shape
9 % Increase in Packing
Density and More Uniformly
Packed Bed
60
59
Density (%)
11.8
27% Improved Avg.

Efficiency
Peak Asymmetry
Efficiency (N)
Density Comparison of
Packed Beds
Reproducible
Column-to-Column Peak Asymmetry
1.2
5.2
58
57
56
55
1.1
54
40,000
Conventional
Slurry Packing
Axia Packed
Hydraulic Piston
Compression Packing
53
Conventional
Slurry Packing
Conventional
Axia Packed
Hydraulic Piston
Compression Packing
Slurry Packing
Axia Packed
Hydraulic Piston
Compression Packing
Compare Lifetime
Axia Packed
Leading Competitive Preparative Column
Luna 5 m C18(2) Axia Packed

mAumAu
Waters XTerra 5 m Prep MS C18 OBD
11
22
mAumAu
33
3 3
2 2
1 1
vs.
500500
0 0
3 3
First Run
4 4
minmin
800th Run
Conditions for all except where noted:
Columns: Luna 5 m C18(2) Axia Packed
Waters XTerra 5 m Prep MS C18 OBD
Dimension: 50 x 21.2 mm (Luna)
50 x 19 mm (XTerra)
Mobile Phase: A: 0.5 % TFA in Water B: 0.5 % TFA in Acetonitrile
Gradient: Linear 95:5 (A/B) to 5:95 (A/B) over 7 min, hold 3 min
App ID 15933
App ID 15972
500500
2 2
AXIA
A completely automated packing system offers feedback control and infinite tuning of packing density to specific media characteristics
such as mechanical strength and porosity. An optimum higher bed density can be consistently reproduced column-to-column. This directly
translates into consistent efficiency and peak asymmetry measurements and decreases the column variability seen in traditionally packed
preparative columns.
0 0
2 2
4 4
3 3
minmin
120th Run
First Run
Six columns tested. Best lifetime chosen for comparison.
Flow Rate: 30 mL/min (Luna)

24 mL/min (XTerra)
Sample: 1. Triprolidine 1.6 mg
2. Methacycline 16 mg
3. Amitriptyline 5.25 mg
SFC
APPROVED
Same linear velocity
Visit www.phenomenex.com/info/Axia to view the technology in action.
Phenomenex
337
U.S. Patent No. 7, 674, 383
Seamless Scalability: 2 Options to Increase Sample Load

Option 1: Increase Column Length
Increase sample load without increasing your flow rate by using
a longer column. With Axia technology, each preparative
column is optimized for:
Analytical-like efficiency
Long column lifetime
High sample load with high-surface area media such as
Gemini, Gemini-NX, Luna, or Synergi
As a result, load generally increases as a direct proportion
to column length. In this example the sample load tripled by
increasing column length.
32 mg
on 100 x 21.2 mm
Axia Packed Column
Gradient: 10 min on 100 mm

length column
25 min on 250 mm
length column
5 % to 95 % Acetonitrile,
0.5 % TFA
Flow Rate: 30 mL/min
96 mg
on 250 x 21.2 mm
Axia Packed Column
min
App ID 18078
App ID 18077
3x load
10
20
min
Option 2: Increase Column ID
AXIA
Increased throughput without sacrificing purity
Columns: Luna 5 m C18(2)

Gradient: 5 min, 5 % to 95 % Water/Acetonitrile, 0.5 % TFA
Flow Rate: 1.5 mL/min on 4.6 mm
30 mL/min on 21.2 mm
60 mL/min on 30 mm
150 mL/min on 50 mm
Sample: 1. Propranolol
2. Diphenhydramine
High efficiency from analytical to preparative
50 x 30 mm
2.4 mg SF=40
Plates/Meter 87120
Asymmetry 1.16
Plates/Meter 83235
Asymmetry 0.97
min
50 x 21.2 mm
1.2 mg SF=20
SFC
SF=Scaling Factor
338
min
Plates/Meter 88280
Asymmetry 1.01
App ID 18063
50 x 50 mm
7.3 mg SF=118
Plates/Meter 84138
Asymmetry 1.17
APPROVED
min
App ID 18065
App ID 18064
50 x 4.6 mm
64 g SF=1
App ID 18062
Reproducible performance across all column

diameters
For maximizing load without increasing the run time, consider

scaling up to a larger column ID. Axia packed columns
provide the three important benefits you need.
Same Efficiency as
Analytical Column
min
Phenomenex
U.S. Patent No. 7, 674, 383
Selectivity Options
Stationary Phase Selectivity
With high surface areas, Phenomenex mediaGemini-NX and Gemini (375 m2/g), Luna (400 m2/g) and Synergi (475 m2/g)maximize
loading capabilities. Use the selection tree below to select the best media for your targeted purification.
Column Selection Tree
Achiral
Yes
Yes
Aromatic?
Gemini C6-Phenyl
(Basic mobile phase pH
recommended)
Chiral
Lux Cellulose-1
Lux Cellulose-2
Lux Cellulose-3
Lux Cellulose-4
Lux Amylose-2
Basic Compounds? No
No
Yes
Aromatic?
No
Synergi Polar-RP
Gemini-NX C18
Gemini C18
Yes
Polar?
Luna Phenyl-Hexyl
(Basic mobile phase

pH recommended)
Gemini-NX C18
Luna C8(2) or C18(2)
Synergi Max-RP
Synergi Polar-RP
Gemini, Lux and Luna are registered trademarks of Phenomenex, Inc.
Synergi Hydro-RP
Synergi is a registered trademark of Phenomenex, Inc.
No
pH-LC Selectivity
In reversed phase chromatography, compounds retain better when neutral. With the advent of pH stable (1-12) media such as GeminiNX C18, Gemini C18 and C6-Phenyl, improving retention and resolution of basic compounds at high pH is now possible without
compromising column lifetime. Under these conditions you can easily double or triple the loading compared to your current low pH
purifications.
Increase Sample Load
pH Selectivity
30 mg load
2500
Low pH
Phenomenex
20000
2500
1500
7.5
min
min
0
3000
1000
min
min
-500
2500 0
500
2.5
1
30 mg load
7.5
1
4
min
2000
0
1500
-500
1000 0
3000
500
pH 10.0
2500
0
2000
-500
3000
1500
High pH
4
1
5
2
2500
1000
2000
500
App ID 17726
App ID 15928
2.5
Increase pH3 for

1
Basic Compounds
500
10000
2000
3
1
1000
min
1500
0
3000
1
1000
-500
2500
2
4
min
Baseline Resolution
for Higher Purity
min
min
500
2000
2
0
1500
Gemini 10 m C18
Axia Packed
2
-500
1000
3000
500
2500
0
60 mg load
2000
3000
1500
2000
500
2
1500
0
2.5
7.5
min
SFC
APPROVED
2500
1000
Column: Gemini 5m C18

Axia Packed
Part No.: 00B-4435-P0-AX
Mobile Phase: A: 50 mM
Ammonium
Bicarbonate, pH 10.0
B: Acetonitrile
Gradient: 95:5 to 5:95 (A/B)
over 5 minutes
2. Diphenhydramine
1000
0
500
App ID 17727
0
2000
-500
1500
App ID 15973
10000
20000
500
2500
Column: Luna 5 m C18(2)

Axia Packed
Mobile Phase: A: 0.5 % TFA in
Water, pH 2.0
B: Acetonitrile
Gradient: 95:5 to 5:95 (A/B)
over 4 minutes
2. Diphenhydramine
App ID 17725
1000
Luna 5 m C18(2)
Axia Packed
High pH
Columns: Gemini 10 m C18
Axia Packed
Mobile Phase: A: 95:5 50 mM
Ammonium Bicarbonate,
pH 10.0 / Acetonitrile
B: 5:95 50 mM
Ammonium Bicarbonate,
pH 10.0 / Acetonitrile
Gradient: 7 min 100 % A to
20000
2
100 % B over
7 min, equilibrate
10000
for 3 min
3
1 Injection Volume: 500 L
0
Flow
Rate: 30
mL/min min
2.5
5
7.5
0
pH 2.0
1500
AXIA
Column: Luna 5 m C18(2)

Axia Packed
20000
B: 0.5 % TFA in
Acetonitrile
Gradient: Linear 95:5 (A/B) to
10000
5:95 (A/B)
over 7 min, hold 3 min
0
0
Low pH
2000
min
min
339
U.S. Patent No. 7, 674, 383
Chiral Media Packed in Axia Technology

Resolve 92% of Your Enantiomers with Lux Chiral Preparative Columns*
Five Distinct Polysaccharide Phases to Screen:
Lux Cellulose-1: Dimethyl Cellulose Chiral Selector Cellulose tris (3, 5-dimethylphenylcarbamate)
Lux Cellulose-2: Chlorinated Cellulose Carbamate Phase Cellulose tris (3-chloro-4-methylphenylcarbamate)
Lux Cellulose-3: Cellulose Ester Chiral Selector Cellulose tris (4-methylbenzoate)
Lux Cellulose-4: Chlorinated Cellulose Carbamate Phase Cellulose tris (4-chloro-3-methylphenylcarbamate)
Lux Amylose-2: Chlorinated Amylose Chiral Selector Amylose tris450(5-chloro-2-methylphenylcarbamate)
* based on screening 233 compounds on the five Lux phases
Availability in 3 m and 5 m packed columns as well as 20 m bulk media for process scale purification
All Lux columns are pressure stable up to 300 bar and pH stable 2-9
400
450
350
400
300
350
250
300
200
250
150
200
Higher Purity Preparative Separations
100
150
50
100
With award-winning Axia technology, analytical-like efficiency is achieved

in a preparative column format.
0
50
High Efficiency trans-Stilbene Oxide from Analytical to Preparative
450
400
150
100
50
0
420
320
300
AXIA
240
10
12
14
16
18
min
10
12
14
16
18
min
10
12
14
16
18
min
10
12
14
16
18
min
21.2 mm ID
N = 77,180
a = 1.22
300
240
180
240
2
2
180
120
120
60
120
0
0
280
240
17780
10
12
14
Conditions for all1 separations except where noted:

Column: Lux 5 m Cellulose-2
Dimensions: 1) 250 x 4.6 mm
2) 250 x 21.2 mm
3) 250 x 50 mm
2
Mobile Phase: Hexane/Isopropanol
(90:10)
Flow Rates: 1) 0.5 mL/min
2) 20 mL/min
3) 50 mL/min
2
4
10
12
Injections:
1)6 2 L 8
2) 10 L
1
3) 30 L
16
18
min
280 0
280
240
Same Efficiency as
Analytical Column
240
200
200
180
50 mm ID
N = 75,156
a = 1.19
180
120
120
80
App ID 18285
80
40
40
0
14
16
18
min
10
12
14
16
18
min
10
12
14
16
18
min
17781
200
Direct
Scale Up of Methylcarbamol on Lux Cellulose-1
180
1
120
Increase ID
80
40
100 x 4.6 mm
5 g
a = 1.9
10
12
Conditions for all separations except where noted:

Column: Lux 5 m Cellulose-1
Dimensions: 1) 100 x 4.6 mm
2) 100 x 21.2 mm
3) 250 x 21.2 mm
Mobile Phase: Methanol/Isopropanol (90:10)
Flow Rates: 1) 1.0 mL/min
2) 20 mL/min
3) 20 mL/min
Detection: 1) UV @ 220 nm
2) UV @ 254 nm
3) UV @ 254 nm
Injections: 1) 5 g in 2 L
2) 32 mg in 640 L
3) 80 mg in 1600 L
14
16
18
min
min
App ID 17781
100 x 21.2 mm
32 mg
a = 1.8
17782
0
11
No Resolution Lost with

Increased Sample Load
min
2.5x Load
Increase
22
App ID 17782
Increase
Length
App ID 17780
60
0
480
340
1
1
320
300
2
App ID 18283
200
SFC
App ID 18284
4.6 mm ID
N = 70,664
a = 1.19
250
APPROVED
420
320
300
60
420
480
350
180
480 0
250 x 21.2 mm
80 mg
a = 1.8
0
min
Phenomenex
U.S. Patent No. 7, 674, 383
Axia: SFC Approved
Seamless Scale Up from Laboratory, to

Pilot Plant and Production.
Complete SFC Screening
Increase column ID for higher loading and greater purification.

Axia packed 21.2 and 30 mm diameter columns provide same
purification capability and performance as the 4.6 mm analytical
screening columns.
From analytical to Axia packed preparative achiral columns,

Luna, Gemini-NX, Gemini, Synergi, and Lux chiral columns offer
complementary selectivities, high efficiency, and pressure stability
up to 300 bar (4300 psi) for SFC separations.
Baseline Separation of Enantiomers

18865
OH
UV-VIS
Lux Cellulose-1 offers great

peak shape at 220 nm
OH
N
Terfenadine
C(CH 3 ) 3
C(CH 3 ) 3
10
20 min
App ID 18865
Polarimeter
5x Load
Increase
18866
UV-VIS

Columns: Lux 5 m Cellulose-1
Mobile Phase: Methanol with 0.1 % DEA/
Carbon Dioxide (25:75)
Column Temperature: 35 C
Polarimeter: ALP-PDR-Chiral
Sample: Terfenadine with ethanol
dissolution solvent
20 min
250 x 4.6 mm
2.5 mL/min
UV @ 254 nm
1.5 mg in 50 L
App ID 18867
Closer stacked injections

can not be used due to the
impurities eluting after the
major enantiomers
Dimensions:
Flow Rate:
Detection:
Load:
250 x 21.2 mm
50 mL/min
UV @ 220 nm
105 mg in 3.5 mL
7.5 cycles
per hr/
787 mg per hr
Dimensions:
Flow Rate:
Detection:
Load:
High loading capacity media

along with stacking injections
allow for increased yields
70x Load
Increase
18867
OH
Polarimeter
OH
Overloading study with

increased analytical load
showing impurities eluting
after major enantiomers only
detected at 254 nm
App ID 18866
10
250 x 4.6 mm
2.5 mL/min
UV @ 220 nm
300 g in 10 L
AXIA
Dimensions:
Flow Rate:
Detection:
Load:
SFC
APPROVED
For more SFC applications, see pp. 295-302
Phenomenex
341

U.S. Patent No. 7, 674, 383
AXIA
Axia Packed Columns
SFC
APPROVED
342
Phases
50 x 21.2 mm
Gemini 5 m C18
Gemini 10 m C18
Gemini-NX 5 m C18
Gemini-NX 10 m C18
Luna 5 m C18(2)
Luna 10 m C18(2)
Luna 5 m C8(2)
Luna 10 m C8(2)
Luna 5 m CN
Luna 5 m PFP(2)
Luna 5 m HILIC
Luna 5 m Silica(2)
Synergi 4 m Max-RP
75 x 21.2 mm
Gemini 5 m C18
Gemini-NX 5 m C18
Luna 5 m C18(2)
100 x 21.2 mm
Gemini 5 m C18
Gemini 10 m C18
Gemini-NX 5 m C18
Gemini-NX 10 m C18
Jupiter 10 m C18
Jupiter 10 m C4
Jupiter 10 m Proteo
Luna 5 m C18(2)
Luna 10 m C18(2)
Luna 5 m C8(2)
Luna 10 m C5
Luna 5 m PFP(2)
Luna 5 m NH2
Luna 5 m HILIC
Luna 5 m Silica (2)
Lux 5 m Amylose-2
Lux 5 m Cellulose-1
Lux 5 m Cellulose-2
Synergi 4 m Max-RP
Synergi 10 m Max-RP
150 x 21.2 mm
Gemini 5 m C18
Gemini 10 m C18
Gemini-NX 5 m C18
Gemini-NX 10 m C18
Luna 5 m C18(2)
Luna 10 m C18(2)
Luna 5 m C8(2)
Luna 10 m C8(2)
Luna 5 m CN
Luna 5 m NH2
Luna 5 m PFP(2)
Luna 5 m HILIC
Luna 5 m Silica(2)
Part No.
Price
00B-4435-P0-AX
00B-4436-P0-AX
00B-4454-P0-AX
00B-4455-P0-AX
00B-4252-P0-AX
00B-4253-P0-AX
00B-4249-P0-AX
00B-4250-P0-AX
00B-4255-P0-AX
00B-4448-P0-AX
00B-4257-P0-AX
00B-4450-P0-AX
00B-4274-P0-AX
00B-4424-P0-AX
00B-4337-P0-AX
00B-4375-P0-AX
00B-4336-P0-AX
00C-4435-P0-AX
00C-4454-P0-AX
00C-4252-P0-AX
00D-4435-P0-AX
00D-4436-P0-AX
00D-4444-P0-AX
00D-4454-P0-AX
00D-4455-P0-AX
00D-4055-P0-AX
00D-4168-P0-AX
00D-4397-P0-AX
00D-4252-P0-AX
00D-4253-P0-AX
00D-4249-P0-AX
00D-4092-P0-AX
00D-4448-P0-AX
00D-4257-P0-AX
00D-4378-P0-AX
00D-4450-P0-AX
00D-4274-P0-AX
00D-4472-P0-AX
00D-4459-P0-AX
00D-4457-P0-AX
00D-4424-P0-AX
00D-4337-P0-AX
00D-4350-P0-AX
00D-4375-P0-AX
00D-4336-P0-AX
00F-4435-P0-AX
00F-4436-P0-AX
00F-4444-P0-AX
00F-4454-P0-AX
00F-4455-P0-AX
00F-4252-P0-AX
00F-4253-P0-AX
00F-4249-P0-AX
00F-4250-P0-AX
00F-4255-P0-AX
00F-4378-P0-AX
00F-4448-P0-AX
00F-4257-P0-AX
00F-4450-P0-AX
00F-4274-P0-AX
auf Anfrage
Inquire
auf Anfrage
Inquire
auf Anfrage
Inquire
Phases
150 x 21.2 mm (contd)
Lux 5 m Amylose-2
Lux 5 m Cellulose-1
Lux 5 m Cellulose-2
Lux 5 m Cellulose-3
Lux 5 m Cellulose-4
Synergi 4 m Max-RP
250 x 21.2 mm
Gemini 5 m C18
Gemini 10 m C18
Gemini-NX 5 m C18
Gemini-NX 10 m C18
Jupiter 10 m C4
Jupiter 4 m Proteo
Jupiter 10 m Proteo
Luna 5 m C18(2)
Luna 10 m C18(2)
Luna 5 m C8(2)
Luna 10 m C8(2)
Luna 10 m C5
Luna 5 m CN
Luna 5 m NH2
Luna 5 m PFP(2)
Luna 5 m Silica(2)
Luna 10 m Silica(2)
Lux 5 m Amylose-2
Lux 5 m Cellulose-1
Lux 5 m Cellulose-2
Lux 5 m Cellulose-3
Lux 5 m Cellulose-4
Synergi 4 m Max-RP
Part No.
Price
00F-4472-P0-AX
00F-4459-P0-AX
00F-4457-P0-AX
00F-4493-P0-AX
00F-4491-P0-AX
00F-4337-P0-AX
00F-4375-P0-AX
00F-4336-P0-AX
00F-4424-P0-AX
auf Anfrage
Inquire
auf Anfrage
Inquire
auf Anfrage
Inquire
auf Anfrage
Inquire
auf Anfrage
Inquire
00G-4435-P0-AX
00G-4436-P0-AX
00G-4444-P0-AX
00G-4454-P0-AX
00G-4455-P0-AX
00G-4168-P0-AX
00G-4396-P0-AX
00G-4397-P0-AX
00G-4252-P0-AX
00G-4253-P0-AX
00G-4249-P0-AX
00G-4250-P0-AX
00G-4092-P0-AX
00G-4255-P0-AX
00G-4378-P0-AX
00G-4448-P0-AX
00G-4257-P0-AX
00G-4274-P0-AX
00G-4091-P0-AX
00G-4472-P0-AX
00G-4459-P0-AX
00G-4457-P0-AX
00G-4493-P0-AX
00G-4491-P0-AX
00G-4337-P0-AX
00G-4375-P0-AX
00G-4336-P0-AX
00G-4424-P0-AX
auf Anfrage
Inquire
auf Anfrage
Inquire
auf Anfrage
Inquire
auf Anfrage
Inquire
auf Anfrage
Inquire
SecurityGuard PREP System

(Highly recommended for extending column lifetime)
Protect your Axia Packed column and prolong its lifetime with SecurityGuard,
the advanced HPLC guard cartridge system.
Get full protection with minimal impact on your chromatographic results.
Contaminants are retained by an inexpensive, 15 x 21.2 or 15 x 30 mm ID
disposable cartridge. See p. 238
SecurityGuard PREP
SecurityGuard PREP System
Part No.
Description
AJ0-8223 SecurityGuard PREP HPLC Guard Cartridge Holder Kit,
21.2 mm ID, includes column coupler
AJ0-8277 SecurityGuard PREP HPLC Guard Cartridge Holder Kit,
30.0 mm ID, includes column coupler
Unit
ea
Price
ea
Phenomenex

U.S. Patent No. 7, 674, 383
Axia Packed Columns (contd)

Ordering Information (contd)
Part No.
Price
00B-4435-U0-AX
00B-4436-U0-AX
00B-4454-U0-AX
00B-4455-U0-AX
00B-4252-U0-AX
00B-4253-U0-AX
00B-4249-U0-AX
00B-4250-U0-AX
00B-4257-U0-AX
00B-4448-U0-AX
00B-4274-U0-AX
00B-4337-U0-AX
00B-4350-U0-AX
00B-4375-U0-AX
00B-4376-U0-AX
00B-4336-U0-AX
00B-4351-U0-AX
00C-4435-U0-AX
00C-4444-U0-AX
00C-4454-U0-AX
00C-4252-U0-AX
00C-4253-U0-AX
00C-4249-U0-AX
00C-4257-U0-AX
00C-4337-U0-AX
00C-4375-U0-AX
00C-4336-U0-AX
For Axia Reducing Adapter, see p. 360

For Clarity Oligo-RP and Oligo-WAX Axia packed columns, see p. 310
For PREP SecurityGuard Cartridges, see p. 238
For PREP Column In-Line Filter, see p. 18
For SFC information see p. 328
For additional sizes not displayed, please visit the individual
product pages or contact your Phenomenex technical
consultant or local distributor.
Phenomenex
Price
00F-4435-U0-AX
00F-4436-U0-AX
00F-4454-U0-AX
00F-4455-U0-AX
00F-4252-U0-AX
00F-4253-U0-AX
00F-4378-U0-AX
00F-4448-U0-AX
00F-4472-U0-AX
00F-4459-U0-AX
00F-4457-U0-AX
00F-4336-U0-AX
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00G-4435-U0-AX
00G-4436-U0-AX
00G-4454-U0-AX
00G-4455-U0-AX
00G-4055-U0-AX
00G-4168-U0-AX
00G-4396-U0-AX
00G-4397-U0-AX
00G-4252-U0-AX
00G-4253-U0-AX
00G-4249-U0-AX
00G-4092-U0-AX
00G-4255-U0-AX
00G-4448-U0-AX
00G-4450-U0-AX
00G-4274-U0-AX
00G-4472-U0-AX
00G-4459-U0-AX
00G-4457-U0-AX
00G-4493-U0-AX
00G-4491-U0-AX
00G-4336-U0-AX
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00B-4435-V0-AX
00B-4436-V0-AX
00B-4455-V0-AX
00B-4252-V0-AX
00B-4253-V0-AX
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00D-4436-V0-AX
00D-4455-V0-AX
00D-4253-V0-AX
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00F-4436-V0-AX
00F-4455-V0-AX
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00G-4436-V0-AX
00G-4455-V0-AX
00G-4253-V0-AX
00G-4273-V0-AX
00G-4250-V0-AX
00G-4272-V0-AX
00G-4092-V0-AX
00G-4472-V0-AX
00G-4459-V0-AX
00G-4457-V0-AX
00G-4493-V0-AX
00G-4491-V0-AX
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Part No.
00D-4435-U0-AX
00D-4436-U0-AX
00D-4444-U0-AX
00D-4454-U0-AX
00D-4455-U0-AX
00D-4055-U0-AX
00D-4397-U0-AX
00D-4252-U0-AX
00D-4253-U0-AX
00D-4249-U0-AX
00D-4255-U0-AX
00D-4378-U0-AX
00D-4448-U0-AX
00D-4257-U0-AX
00D-4274-U0-AX
00D-4472-U0-AX
00D-4459-U0-AX
00D-4457-U0-AX
00D-4337-U0-AX
00D-4350-U0-AX
00D-4375-U0-AX
00D-4336-U0-AX
00D-4424-U0-AX
00D-4425-U0-AX
Phases
150 x 30 mm
Gemini 5 m C18
Gemini 10 m C18
Gemini-NX 5 m C18
Gemini-NX 10 m C18
Luna 5 m C18(2)
Luna 10 m C18(2)
Luna 5 m NH2
Luna 5 m PFP(2)
Lux 5 m Amylose-2
Lux 5 m Cellulose-1
Lux 5 m Cellulose-2
250 x 30 mm
Gemini 5 m C18
Gemini 10 m C18
Gemini-NX 5 m C18
Gemini-NX 10 m C18
Jupiter 10 m C18
Jupiter 10 m C4
Jupiter 4 m Proteo
Jupiter 10 m Proteo
Luna 5 m C18(2)
Luna 10 m C18(2)
Luna 5 m C8(2)
Luna 10 m C5
Luna 5 m CN
Luna 5 m PFP(2)
Luna 5 m HILIC
Luna 5 m Silica(2)
Lux 5 m Amylose-2
Lux 5 m Cellulose-1
Lux 5 m Cellulose-2
Lux 5 m Cellulose-3
Lux 5 m Cellulose-4
50 x 50 mm
Gemini 5 m C18
Gemini 10 m C18
Gemini-NX 10 m C18
Luna 5 m C18(2)
Luna 10 m C18(2)
100 x 50 mm
Gemini 10 m C18
Gemini-NX 10 m C18
Luna 10 m C18(2)
150 x 50 mm
Gemini 10 m C18
Gemini-NX 10 m C18
250 x 50 mm
Gemini 10 m C18
Gemini-NX 10 m C18
Luna 10 m C18(2)
Luna 15 m C18(2)
Luna 10 m C8(2)
Luna 15 m C8(2)
Luna 10 m C5
Lux 5 m Amylose-2
Lux 5 m Cellulose-1
Lux 5 m Cellulose-2
Lux 5 m Cellulose-3
Lux 5 m Cellulose-4
AXIA
Phases
50 x 30 mm
Gemini 5 m C18
Gemini 10 m C18
Gemini-NX 5 m C18
Gemini-NX 10 m C18
Luna 5 m C18(2)
Luna 10 m C18(2)
Luna 5 m C8(2)
Luna 10 m C8(2)
Luna 5 m PFP(2)
Luna 5 m Silica(2)
Synergi 4 m Max-RP
Synergi 10 m Max-RP
75 x 30 mm
Gemini 5 m C18
Gemini-NX 5 m C18
Luna 5 m C18(2)
Luna 10 m C18(2)
Luna 5 m C8(2)
Synergi 4 m Max-RP
100 x 30 mm
Gemini 5 m C18
Gemini 10 m C18
Gemini-NX 5 m C18
Gemini-NX 10 m C18
Jupiter 10 m C18
Jupiter 10 m Proteo
Luna 5 m C18(2)
Luna 10 m C18(2)
Luna 5 m C8(2)
Luna 5 m CN
Luna 5 m NH2
Luna 5 m PFP(2)
Luna 5 m Silica(2)
Lux 5 m Amylose-2
Lux 5 m Cellulose-1
Lux 5 m Cellulose-2
Synergi 4 m Max-RP
Synergi 10 m Max-RP
SFC
APPROVED
343
Process Chromatography
Bulk HPLC Media
Mechanically Strong Media
Grams to Multi-Kilogram, Phenomenex can deliver
Media free of crushed or cracked silica and silica fines
Over 20 different media available
Backpressures that remain stable
Long lifetime and excellent reproducibility
Consistent particle size distribution so performance is

maintained
Quick, Direct Scale-up from Analytical

Methods
Longer column lifetimes (frits stay unclogged)
Scaling up is easier when using an HPLC media that provides near

identical performance across all particle sizes and with increases
in column diameter. Any mobile phase conditions developed on a
Gemini,
Jupiter, Luna or Synergi analytical column can be easily
mAU
transferred to a 10 m or 15 m preparative column with equivalent
600
resolution, selectivity, and proportional mass loading. Lux analytical
400
columns
also easily scale to 20 m preparative columns.
200
0
Equivalent
Performance
Across
All Particle Sizes
0
2
4
6
min
Withstand Multiple Repacking in Dynamic Axial Compression

(DAC) Systems
Dynamic Axial Compression (DAC) systems apply high mechanical
stress on the packing media. This, along with high flow rates and
backpressures can crack or sheer low mechanical strength silica
particles, creating silica fines, which will rapidly degrade column
efficiency and clog frits. Gemini, Jupiter, Luna, Synergi and Lux
media provide exceptional strength over multiple DAC packings
without sacrificing performance as well as easily withstanding high
mechanical stress.
mAU
600
Luna 5 m C18(2)
400
200
0
PROCESS CHROMATOGRAPHY
min
mAU
400
Luna 10 m C18(2)
200
0
0
min
mAU
400
Luna 15 m C18(2)
200
0
0
Scaling Up with Luna 10 m C18(2)
SFC
344
8 min
0.93 mg Berberine (1)

1.82 mg Indoprofen (2)

150 x 21.2 mm
flow rate = 20 mL/min
10 min
150 x 10 mm
flow rate = 4.7 mL/min
150 x 4.6 mm
flow rate = 1.0 mL/min
APPROVED
min
8 min

Phenomenex
2500
2000
1500
1000
500
Chemically Stable Media
Increase Sample Load with pH

0
-500
Chemical stability at pH levels outside the normal constraints of

2-7 is a critical factor in todays process environments for several
reasons:
Allows greater loading capacity
2500
In reversed phase chromatography, compounds retain better when

neutral. With the advent of pH stable (1-12) process medias such as
Gemini C18, improving retention and resolution of your basic compounds at high pH is now possible without compromising column
lifetime. Under these conditions you can easily double or triple the
loading compared to your current low pH purifications.
1
min
min
3000
Allows optimization of sample solubility
2500
2000
2000
1500
pH adjustment to optimize recovery of API
1500
1000
Clean-in-Place (CIP) processes by means of a caustic wash
1000
500
500
0
-500
min
Increase pH for basic compounds
Luna 30 mg load
pH 2
2500
Gemini 30 mg load
pH 10
3000
Improved
Resolution!
2500
2000
2000
-500
Column:
Dimensions:
2500
Part No.:
2000
Mobile Phase:
1500
0
-500
1000
500
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:
0
Luna 5 m C18(2) Axia Packed

1
50 x 21.2 mm
00B-4252-P0-AX
A: 0.5 % TFA in Water, pH 2.0
B: Acetonitrile
95:5 to 5:95 (A/B) over 42minutes
30 mL/min
Ambient
UV @ 254 nm
1. Propranolol
2
3
4
5
2. Diphenhydramine
min
High pH
Column:
Dimensions:
Part No.:
Mobile Phase:
3000
2500
2000
1500
1000
500
0
min
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:2
1
min
min
Low pH
500
500
1500
-500
3000
1000
1000
App ID 17726
App ID 17725
500
2000
1500
2
3000
2500
1500
1000
Gemini 60 mg load
pH 10
2x load
App ID 17727
-500
Gemini 5 m C18
Axia Packed
1
50 x 21.2 mm
00B-4435-P0-AX
A: 50 mM Ammonium
Bicarbonate, pH 10.0
2
B: Acetonitrile
95:5 to 5:95 (A/B) over 5 minutes
30 mL/min
Ambient
UV @ 254 nm
1. Propranolol
3
4
5
min
2. Diphenhydramine
3000
Luna 5 m C18(2) 100 , pH Stable (1.5-10)
Gemini 5 m C18 110 , pH Stable (1-12)
2500
2000
16000
1500
500
0
pH 10.0
3
12000
10000
pH 11.5
8000
min
25
tR Retention Time (min)
14000
Efficiency (N)
1000
20
15
10
SFC
APPROVED
10 14 18 22 26 30 34 38 42 46 50 54 58 62 66 70 74 78 82 86 90 94 98
Hours of Exposure
Days of Exposure
Test Conditions: Column stability tested under highly basic conditions

Continuous flush in 20 mM Na2HPO4 (pH 10.0) in Water/Acetonitrile, 50:50.
Test Conditions: Flushed with non recycled buffer 1:1 Acetonitrile 50 mM

Methylpyrrolidine (pH 11.5), 1 mL/min.
KEY:
KEY:
Efficiency, Plates for Toluene
tR of Toluene (min)
Symmetry of Toluene
Symmetry of Pyridine
8000
Extended Media Lifetime even Under Caustic Washes
k of Toluene
tR of Toluene
tR Diphenhydramine
tR Propranolol
tR Nortriptyline
Phenomenex
5
345
Excellent Performance at Low pH
Luna 5 m C18(2) 100 , pH Stable (1.5-10)
Gemini 5 m C18 110 , pH Stable (1-12)

Efficiency (N)
14000
pH 1.5
12000
10
10000
9
8
pH 1.5
tR, Retention Time (min)
7
6
5
4
3
2
1
Hours of Exposure
0
0
10
15
20
25
30
Days of Exposure
Test Conditions: Column stability tested under highly acidic conditions.

Continuous flush in 0.1 % TFA (pH 1.5) in Water/Acetonitrile, 50:50.
Test Conditions: Continuous flush in 0.1 % TFA, (pH 1.5) at room temperature.
KEY:
KEY:
Efficiency, Plates for Toluene
tR of Toluene (min)
Symmetry of Toluene
Symmetry of Pyridine
8000
We engineer and manufacture all of our media with your needs

as a guideline.
Our state-of-the-art facility gives us the capability
3
to provide some of the most consistent media available on the
market. With very high loadability, excellent mechanical strength,
extended 1chemical stability, and batch-to-batch reproducibility,
it is no wonder why more and more people turn to Phenomenex
media every day. 500
1000
1500
2000
2500
A Certificate of Analysis showing the test results for the
batch of material is provided with each Gemini media order.
This data ensures batch-to-batch reproducibility.
tR Tolune
tR Ethylparaben
tR Diflunisal
1.90
2.70
500
1000
1500
2000
1.70
2500
2.20
1.50
1.70
1.30
1.10
1.20
0.90
0.70
0.70
2.50
0.20
0.50
5293-55
5293-53
5293-51
5293-49
5293-47
5293-45
5293-43
5293-41
5293-39
5293-37
5293-35
5293-33
5293-31
5293-29
5293-27
5293-25
5293-23
5293-21
5293-19
5293-17
5293-15
5293-13
5293-11
5293-9
5293-7
5293-5
2.00
5293-3
5293-1
Gemini 10 m C18 Specification Ranges
3.00
tR Naproxen
Batch-to-Batch5 Reproducibility
With over 15 years of proven reproducibility, you can be
confident in your choice to develop methods on Luna. The
following 3graph shows consistency in both inertness and
hydrophobicity across 40 batches of Luna 10 m C18(2).
Metal Test and Inertness
Controlled Manufacturing Process
Luna 10 m C18(2) Batch Number
1.50
1.00
0.50
Hydrogen Bonding
Hydrophobic Index
Metal Test
Inertness
0.00
Gemini 10 m C18 Batch Number

Metal Sensitivity
Silanol Activity
Methylene Selectivity
Metal Sensitivity
Silanol Activity
Methylene Selectivity
5521-75
5521-73
5521-70
5521-66
5521-64
5521-62
5521-60
5521-58
5521-56
5521-54
5521-52
5521-50
5521-48
5521-46
5521-43
5521-39
5521-36
5521-33
5521-29
5521-27
5521-25
5521-23
SFC
APPROVED
k of Toluene
Hydrogen Bonding and Hydrophobic Index
8000
Exceptional Chemical Stability for Low Leachates

The dense bonded phase density of Luna and Synergi provide
revolutionary pH 1.510 stability, with Gemini offering an
extended pH range of 1.0-12.0. The wide pH range of these
media provides flexibility in method development allowing for
improvements in resolution and greater mass loading of basic
compounds (pKa > 9) at high pH.
Please see Sorbent Characteristics chart pp. 388-389 for exact pH limits of each phase.
346
Phenomenex
Less Media Needed for Column Packing
Packing Density for Common Prep LC Medias
Packing Media
Packing Density (g/cm3)
Media Required to Pack a 250 x 50 mm Column (g)
0.358
0.433
0.448
0.503
0.508
0.540
0.544
0.566
0.580
0.580
0.647
0.666
176
212
220
247
250
265
267
278
285
285
317
327
Jupiter 300 10 m C4 300

Jupiter 300 10 m C18 300
Luna 10 m Silica(2) 100
YMC 10 m ODS-A 300
Kromasil 10 m Silica 100
Synergi 10 m Polar-RP 80
Vydac 10 m C4 300
Luna 10 m C18(2) 100
Gemini 10 m 110
Synergi 10 m Max-RP 80
LiChrospher 12 m RP-18 100
Kromasil 10 m C18 100
The packing density of each media was determined by packing 100 g into a 50 mm ID DAC column and measuring the resulting bed height. The packing density was
calculated as the ratio of sorbent weight over packed bed volume.
Calculated amount of media (g) required for packing a column with the dimensions L x ID (mm) by axial compression: (ID/20)2 x (3.14)(L/10)(packing density)
Luna vs. Kromasil - Bulk HPLC Media
Kromasil
10 m C18
5kg
20kg
20kg
20kg
20kg
= 85 kg
15 %
Media
Savings
20kg
20kg
20kg
20kg
20kg
= 100 kg
Luna
10 m C18(2)
Jupiter 300 vs. Vydac - Bulk HPLC Media
Jupiter
10 m 300 C4
20kg
20kg
14kg
= 54 kg
34 %
Media
Savings
2kg
Vydac
10 m 300 C4
20kg
20kg
20kg
20kg
150 L Column Volume
= 82 kg
150 L Column Volume
SFC
APPROVED
Phenomenex
347
Prep LC Columns and
Bulk HPLC Media
Maintain or Increase Yield with

Less Media
Higher silica surface area equals greater mass loading. With
400m2/g surface area, Luna has one of the highest surface areas
among popular Prep LC media. Even greater mass loading is possible with the 475m2/g surface area of Synergi 80 media. Both
Synergi and Luna are unique in that they offer high mass loading
with low-density, rugged silica; requiring less media to pack a given
volume. Thus while less media is needed to pack a given dimension
compared to other common prep sorbents, mass loading remains
high with peak resolution and purity maintained. Especially for early
eluting target compounds, Luna has been shown to provide greater
mass loading compared to some common prep media. This allows
for increased loading on less media, and more yield per run.
Maintain or increase yield with less media

Dramatically reduce cost of Prep/Process-LC
purifications
Withstand multiple axial compression packings
with high mechanical strength media
Choose the Correct Media for your Application

Sorbent
Pore
Size
()
Surface
Area
(m2/g)
pH
Stability
Si (Silica)
Luna Silica(2)
100
400
2.0 - 7.5
C18
Luna C18(2)
100
400
1.5 - 10
Synergi Hydro-RP
C18 with Polar
Endcapping
Jupiter 300 C18
80
475
1.5 - 7.5
300
170
1.5 - 10
10 m (bulk),
15 m (bulk)
Hydrophilic Proteins, Oligonucleotides (>30 mer)
C12
Gemini C18
Synergi Max-RP
110
80
375
475
1-12
1.5 - 10
10 m (bulk)
10 m (bulk)
C8
Jupiter Proteo
Luna C8(2)
90
100
475
400
1.5 - 10
1.5 - 10
C5
Luna C5
Jupiter 300 C5
100
300
440
170
1.5 - 10
1.5 - 10
C4
Jupiter 300 C4
300
170
1.5 - 10
Phenyl
Luna Phenyl-Hexyl
100
440
1.5 - 10
80
475
1.5 7.0
CN (Cyano)
Synergi Polar-RP
(Ether-Linked Phenyl)
Luna CN
10 m (bulk)
10 m (bulk)
10-PREP (bulk)
15 m (bulk)
10 m (bulk)
10 m (bulk),
15 m (bulk)
10 m (bulk),
15 m (bulk)
10 m (bulk)
10-PREP (bulk)
15 m (bulk)
10 m (bulk)
Basic compounds, increased retention & loadability at high pH

Pharmaceuticals, Nutraceuticals, Agrochemical, Vitamins, AminoAcids, Basic Compounds, General Reversed-Phase Applications
Peptides, Oligonucleotides (<30 mer), siRNA
Pharmaceuticals, Estrogens, Basic Compounds, General ReversedPhase Applications
100
400
1.5 7.0
10 m (bulk)
NH2 (Amino)
Luna NH2
100
400
1.5 11
10 m (bulk)
SCX (Strong Cation

Exchange)
Luna SCX
100
400
2 7.0
10 m (bulk)
Lux Cellulose-1
1000
29
20 m
Enhanced enantioselectivity for aromatic, conjugated and other chiral

compounds
Lux Cellulose-2
1000
29
20 m

compounds
Lux Cellulose-3
1000
29
20 m
Lux Cellulose-4
1000
29
20 m

compounds
compounds
Lux Amylose-2
1000
29
20 m
Bonded Phase
Particle Size (m)

("bulk" indicates bulk
media available)
Applications
Achiral Media
10 m (bulk)
10-PREP (bulk)
15 m (bulk)
10 m (bulk)
10-PREP (bulk)
15 m (bulk)
10 m (bulk)
Small Organic Molecules, Nutraceuticals, Fat Soluble Vitamins,

Tocopherols
Pharmaceuticals, Nutraceuticals, Agrochemical, Vitamins, Basic
Compounds, General Reversed-Phase Applications
Very Polar Compounds, Pharmaceuticals, Vitamins, Antibiotics
Polar Compounds
Hydrophobic Proteins
Hydrophobic Proteins
Polar and Aromatic Compounds, Antibiotics, Lipids, Phenols,
Sweeteners
Polar and Aromatic Compounds, Antibiotics, Phenols, Sweeteners
Pharmaceuticals, Esters, Steroids, Phthalates, Compounds with
COOH, CO, NH2, NHR2 or NR2 groups
Sugars, Sugar Alcohols, Anionic Compounds, Steroids, Vitamins,
Nucleosides, Oligonucleotides
Amine and polyamine containing compounds, Nucleotides, Peptides
Chiral Media
SFC
APPROVED
348
cellulose tris(3,5dimethylphenyl
carbamate)
cellulose tris(3chloro-4-methyl
phenylcarbamate)
cellulose tris(4-methyl
benzoate)
cellulose tris(4chloro-3-methyl
phenylcarbamate)
amylose tris(5chloro-2-methyl
phenylcarbamate)

compounds
Phenomenex
Screening for Best Selectivity
The extensive range of high surface area stationary phases we
offer in bulk are suitable for the purification of a wide range of
compounds of small or large molecular weight by process HPLC.
To expedite the process of identifying the best combination of
stationary phase and mobile phase additive providing the best
selectivity for highest load of the sample at hand, analytical size
scout columns are tested on Phenomenex column selectors.
Mobile Phase Screening
In the example below, several phases were screened for favorable

selectivity characteristics for a decapeptide of medium polarity.
Optimization of mobile phase buffers and organic content is now

possible to ensure the best separation.
13.156
12.921
min
12.081
20
min
20
10
min
20
1.11
10
12.513
Synergi Fusion-RP
Polar Embedded C18
15
10
TEAP
15
10
1.12
HCI
15
10
Luna
C5
0
min
20
20
min
15
20
min
Methanol
15
20
min
1.12
0
15
20
min
10
45 to 65 % B in 30 min; flow rate

1.0 mL/min; 30 L of 1 mg/mL crude
Luna C18(2) demonstrated the best selectivity amongst the alkyl

phases screened, but our unique polar embedded phase, Synergi
Fusion-RP provides the best overall resolution in combination with
TFA as mobile phase additive.
In this example, Synergi Fusion-RP in TFA provides the best overall

separation. With a thorough column screening approach completed, it
becomes a simple task to calculate loading capacity and scale up to
the dimensions required for your purification.
Purification Partnership
Phenomenex Technical Support and Purification Partnership
commitment provides all the support and expertise you would
expect of a premium vendor: high quality products and service.

10
15
min
20
15
10
NH4OAc
15
10
Luna
C8
0
13.265
min
20
13.145
15
10
1.12
12.683
Synergi Max-RP
C12
TFA
1.09
14.157
13.275
Luna
C18(2)
13.026
Sorbent Screening
Our strong commitment to you and your application extends to:

Fast effective method development instruction to
optimize API resolution
Industry expertise for modeling scale-up from analytical
to preparative column packing instrument expertise
SFC
APPROVED
A detailed future media inventory plan based upon your

predicted manufacture of API
Continuing support and contact throughout purification
Additional training and workshops designed to suit your
requirements
Phenomenex
349
Scout and Axia-packed Preparative Columns
Achiral Columns
Luna 10 m Semi-Prep and Axia Preparative Columns (mm)
Phases
250 x 4.6
250 x 10
250 x 15
250 x 21.2
Silica(2)
C5
C8(2)
C18(2)
CN
Phenyl-Hexyl
00G-4091-E0
00G-4092-E0
00G-4250-E0
00G-4253-E0
00G-4300-E0
00G-4285-E0
00G-4091-N0
00G-4091-P0-AX
00G-4092-N0
00G-4092-P0-AX
00G-4250-N0 00G-4250-AK 00G-4250-P0-AX
00G-4253-N0 00G-4253-AK 00G-4253-P0-AX
00G-4300-N0
00G-4300-P0-AX
00G-4285-N0
00G-4285-P0-AX
250 x 30
250 x 50
aufInquire
Anfrage
aufInquire
Anfrage
00G-4091-U0-AX 00G-4091 V0-AX
00G-4250-U0-AX 00G-4250-V0-AX
00G-4253-U0-AX 00G-4253-V0-AX
00G-4285-U0-AX

4 x 3.0*
10 x 10
15 x 21.2**
/10pk
/3pk
/ea
AJ0-4348
AJ0-7223
AJ0-7229
AJ0-4293
AJO-7372
AJ0-4290
AJ0-7222
AJ0-7840
AJ0-4287
AJ0-7221
AJ0-7839
AJ0-4305
AJ0-7313
AJ0-8220
AJ0-4351
AJ0-7314
AJ0-7841
for ID:3.2-8.0 mm
Luna 10 m-PREP Semi-Prep and Axia Preparative Columns (mm)

Phases
250 x 4.6
250 x 10
250 x 21.2
PREP C18(2)
00G-4324-E0
00G-4324-N0
250 x 30
aufInquire
Anfrage
00G-4324-P0-AX 00G-4324-U0-AX
4 x 3.0*
/10pk
AJ0-4287
9-16 mm
Gemini 10 m Semi-Prep and Axia Preparative Columns (mm)

Phases
250 x 4.6
250 x 10
250 x 21.2
250 x 30
250 x 50
Inquire
aufInquire
Anfrage
aufInquire
Anfrage
C18
00G-4436-E0
00G-4436-N0 00G-4436-P0-AX 00G-4436-U0-AX 00G-4436-V0-AX
18-29 mm
18-29 mm
4 x 3.0*
/10pk
AJ0-7597
250 x 21.2
C4 300
C5 300
C18 300
Proteo 90
00G-4168-E0
00G-4054-E0
00G-4055-E0
00G-4397-E0
00G-4168-N0
00G-4054-N0
00G-4055-N0
00G-4397-N0
00G-4397-AK
00G-4168-P0-AX
00G-4397-P0-AX
250 x 30
aufInquire
Anfrage
00G-4168-U0-AX
00G-4397-U0-AX
30-49 mm
4 x 3.0*
/10pk
AJ0-4330
AJ0-4327
AJ0-4321
AJ0-6074
9-16 mm
Max-RP
Polar-RP
Hydro-RP
Fusion-RP
00G-4350-E0
00G-4351-E0
00G-4376-E0
00G-4425-E0
00G-4350-N0
00G-4351-N0
00G-4376-N0
00G-4425-N0
00G-4376-AK
00G-4350-P0-AX
00G-4351-P0-AX
00G-4376-P0-AX
00G-4425-P0-AX
18-29 mm
9-16 mm
15 x 30
/ea
AJ0-8308
30-49 mm

10 x 10
15 x 21.2**
/3pk
/ea
AJ0-7225
AJ0-7231
AJ0-7371
AJ0-7224
AJ0-7230
AJ0-7275
AJ0-7842
for ID: 3.2-8.0 mm
Synergi 10 m Analytical, Semi-Prep, Axia Preparative and Pilot Scale Columns (mm)
Phases
250 x 4.6
250 x 10
250 x 15
250 x 21.2
30-49 mm

10 x 10
15 x 21.2**
/3pk
/ea
AJ0-7598
AJ0-7846
for ID: 3.2-8.0 mm
Jupiter 10 m Semi-Prep and Axia Preparative Columns (mm)

Phases
250 x 4.6
250 x 10
250 x 15
30-49 mm
SecurityGuard
SecurityGuard
Cartridges
Cartridges
(mm) (mm)
4 x 3.0*
15 x 21.2**
15 x 30
/10pk
/ea
/ea
AJ0-4348
AJ0-7229
AJ0-8312
AJ0-4290
AJ0-7840
AJ0-8302
AJ0-4287
AJ0-7839
AJ0-8301
for ID: 3.2-8.0 mm
250 x 50
aufInquire
Anfrage
00G-4272-V0-AX
00G-4273-V0-AX
18-29 mm

10 x 10.0 15 x 21.2**
15 x 30
/3pk
/ea
/ea
AJ0-7221
AJ0-7839
AJ0-8301
for ID: 3.2-8.0 mm
Luna 15 m Axia Preparative and Pilot Scale Columns (mm)

Phases
250 x 4.6
250 x 21.2
250 x 30
aufInquire
Anfrage
Silica(2)
00G-4271-E0
00G-4271-P0-AX 00G-4271-U0-AX
C8(2)
00G-4272-E0
00G-4272-P0-AX
C18(2)
00G-4273-E0
00G-4273-P0-AX 00G-4273-U0-AX
9-16 mm
15 x 30
/ea
AJ0-8312
AJ0-8302
AJ0-8301
AJ0-8311
AJ0-8303
18-29 mm
15 x 30
/ea
AJ0-8314
AJ0-8313
AJ0-8304
30-49 mm

10 x 10
15 x 21.2 **
/3pk
/ea
AJ0-7275
AJ0-7842
AJ0-7276
AJ0-7845
AJ0-7512
AJ0-7843
AJ0-7558
AJ0-7844
250 x 30
aufInquire
Anfrage
00G-4350-U0-AX
00G-4351-U0-AX
00G-4376-U0-AX
00G-4425-U0-AX
4 x 3.0*
/10pk
AJ0-6074
AJ0-6076
AJ0-7511
AJ0-7557
for ID: 3.2-8.0 mm
Chiral Columns
9-16 mm
18-29 mm
15 x 30
/ea
AJ0-8304
AJ0-8307
AJ0-8305
AJ0-8306
30-49 mm
SFC
APPROVED
Lux 20 m Analytical and Axia Preparative Columns (mm)

Phases
150 x 4.6
250 x 4.6
250 x 21.2
aufInquire
Anfrage
Lux Cellulose-1
00F-4473-E0
00G-4473-E0
00G-4473-P0-AX
Lux Cellulose-2
00F-4464-E0
00G-4464-E0
00G-4464-P0-AX
Lux Cellulose-3
00F-4504-E0
00G-4504-E0
00G-4504-P0-AX
Lux Cellulose-4
00F-4503-E0
00G-4503-E0
00G-4503-P0-AX
250 x 30
aufInquire
Anfrage
00G-4473-U0-AX
00G-4464-U0-AX
250 x 50
aufInquire
Anfrage
00G-4473-V0-AX
00G-4464-V0-AX
00G-4504-V0-AX
00G-4503-V0-AX

4 x 3.0*
15 x 21.2**
15 x 30
/10pk
/ea
/ea
AJ0-8403
AJ0-8405
AJ0-8406
AJ0-8366
AJ0-8400
AJ0-8401
AJ0-8622
AJ0-8624
AJ0-8625
AJ0-8627
AJ0-8629
AJ0-8630
for ID: 3.2-8.0 mm
For additional sizes not listed, please visit the individual

product pages or contact your Phenomenex technical consultant
or local distributor.
350
18-29 mm
30-49 mm

Phenomenex
Bulk HPLC Media
Achiral Media
Gemini (110 )
Phases
10 m
C18
1 kg
aufInquire
Anfrage
04K-4436
5 kg
aufInquire
Anfrage
04L-4436
10 kg
aufInquire
Anfrage
04M-4436
5 kg
aufInquire
Anfrage
04L-4091
04L-4250
04L-4253
04L-4300
04L-4379
04L-4401
04L-4285
aufInquire
Anfrage
04L-4322
04L-4323
04L-4324
04L-4325
aufInquire
Anfrage
04L-4271
04L-4272
04L-4273
04L-4286
10 kg
aufInquire
Anfrage
04M-4091
04M-4250
04M-4253
04M-4300
04M-4379
04M-4401
04M-4285
aufInquire
Anfrage
04M-4322
04M-4323
04M-4324
04M-4325
aufInquire
Anfrage
04M-4271
04M-4272
04M-4273
04M-4286
50 kg
aufInquire
Anfrage
04N-4091
04N-4250
04N-4253
04N-4300
04N-4379
04N-4401
04N-4285
aufInquire
Anfrage
04N-4322
04N-4323
04N-4324
04N-4325
aufInquire
Anfrage
04N-4271
04N-4272
04N-4273
04N-4286
100 kg
aufInquire
Anfrage
04P-4091
04P-4250
04P-4253
04P-4300
04P-4379
04P-4401
04P-4285
aufInquire
Anfrage
04P-4322
04P-4323
04P-4324
04P-4325
aufInquire
Anfrage
04P-4271
04P-4272
04P-4273
04P-4286
Jupiter (300 and 90 )

Phases
100 g
10 m
aufInquire
Anfrage
300 C4
04G-4168
300 C5
04G-4054
300 C18
04G-4055
Proteo 90
04G-4397
15 m
aufInquire
Anfrage
300 C4
04G-4169
300 C5
04G-4056
300 C18
04G-4057
1 kg
aufInquire
Anfrage
04K-4168
04K-4054
04K-4055
04K-4397
aufInquire
Anfrage
04K-4169
04K-4056
04K-4057
5 kg
aufInquire
Anfrage
04L-4168
04L-4054
04L-4055
04L-4397
aufInquire
Anfrage
04L-4169
04L-4056
04L-4057
10 kg
aufInquire
Anfrage
04M-4168
04M-4054
04M-4055
04M-4397
aufInquire
Anfrage
04M-4169
04M-4056
04M-4057
50 kg
aufInquire
Anfrage
04N-4168
04N-4054
04N-4055
04N-4397
aufInquire
Anfrage
04N-4169
04N-4056
04N-4057
100 kg
aufInquire
Anfrage
04P-4168
04P-4054
04P-4055
04P-4397
aufInquire
Anfrage
04P-4169
04P-4056
04P-4057
1 kg
aufInquire
Anfrage
04K-4350
04K-4376
04K-4351
04K-4425
5 kg
aufInquire
Anfrage
04L-4350
04L-4376
04L-4351
04L-4425
10 kg
aufInquire
Anfrage
04M-4350
04M-4376
04M-4351
04M-4425
50 kg
aufInquire
Anfrage
04N-4350
04N-4376
04N-4351
04N-4425
100 kg
aufInquire
Anfrage
04P-4350
04P-4376
04P-4351
04P-4425
100 g
aufInquire
Anfrage
04G-4473
04G-4464
04G-4504
04G-4503
1 kg
aufInquire
Anfrage
04K-4473
04K-4464
04K-4504
04K-4503
Synergi (80 )
Phases
10 m
Max-RP
Hydro-RP
Polar-RP
Fusion-RP
100 g
aufInquire
Anfrage
04G-4350
04G-4376
04G-4351
04G-4425
1 kg
aufInquire
Anfrage
04K-4091
04K-4250
04K-4253
04K-4300
04K-4379
04K-4401
04K-4285
aufInquire
Anfrage
04K-4322
04K-4323
04K-4324
04K-4325
aufInquire
Anfrage
04K-4271
04K-4272
04K-4273
04K-4286
100 g
aufInquire
Anfrage
04G-4091
04G-4250
04G-4253
04G-4300
04G-4379
04G-4401
04G-4285
aufInquire
Anfrage
04G-4322
04G-4323
04G-4324
04G-4325
aufInquire
Anfrage
04G-4271
04G-4272
04G-4273
04G-4286
Luna (100 )
Phases
10 m
Silica(2)
C8(2)
C18(2)
CN
NH2
SCX
Phenyl-Hexyl
10 m-PREP
Silica(2)
C8(2)
C18(2)
Phenyl-Hexyl
15 m
Silica(2)
C8(2)
C18(2)
Phenyl-Hexyl
100 g
aufInquire
Anfrage
04G-4436
Chiral Media
Lux (1000 )
Phases
20 m
Lux Cellulose-1
Lux Cellulose-2
Lux Cellulose-3
Lux Cellulose-4
10 g
aufInquire
Anfrage
04D-4473
04D-4464
04D-4504
04D-4503
Contact your Phenomenex technical consultant or local distributor

for additional bulk packings and quantities not listed.
SFC
APPROVED
For additional brand information, please visit the individual

product pages or contact your Phenomenex technical
consultant or local distributor
For Sepra bulk sorbents, see p. 352
Phenomenex
351
If Sepra Bulk products do not perform
within 45 days for a full refund.
Sepra Bulk Sorbents

Provides reproducible recoveries from capture to purification
Removes contaminants and eliminates matrix effects
Offers controlled selectivity for target analytes
Results in high-throughput sample purification
Phenomenex offers a wide mix of bulk media including an array of large particle media for todays chemists who
need effective capture and concentrating resins.
Sepra media offers purification of proteins, peptides, nucleic acids, antibodies, tryptic digests, nucleotides,
viruses, and small molecular weight pharmaceuticals in a low pressure environment. It is an excellent economical alternative to high pressure RPC while still offering high resolution and loading capacity.
PROCESS CHROMATOGRAPHY | SEPRA BULK SORBENTS
Capture and Concentrate Resins

Media Base Material
Silica
Particle
Size
(m)
Pore Size
()
Surface
Area
(m2/g)
Carbon
Load
(%)
pH Stability
Florisil
SCX
SAX
WCX
Silica
Polymer SDB-L
50
50
50
50
50
50
170 (60/100 mesh)
50
50
55
50
95
65
135
65
65
65
65
80
65
65
70
65
255
500
300
500
500
500
500
300
500
500
500
500
500
17
15
10
10
10
5
0
9
6
8
0
2-9
2-9
2-9
2-9
2-9
2-9
2-9
2-9
2-9
2-9
2-9
1-14
ZT
ZT-SCX
ZT-WCX
ZT-WAX
30
30
30
30
85
85
85
85
800
800
800
800
1-14
1-14
1-14
1-14
ZTL
ZTL-SCX
ZTL-WAX
115
115
115
330
330
330
500
500
500
1-14
1-14
1-14
Brand
Phase
Sepra
C18-E
C18-T
C8
Phenyl
CN
NH2
Polymer
Sepra ZT
Large Particle Polymer Sepra ZTL
Sepra Bulk Sorbents
Brand
Sepra
SFC
APPROVED
Phase
100 g
Price
1 kg
Price
C18-E
C18-T
C8
Phenyl
CN
NH2
Florisil
SCX
SAX
WCX
Silica
SDB-L
04G-4348
04G-4405
04G-4406
04G-4407
04G-4409
04G-4408
04G-4411
04G-4413
04G-4414
04G-SO27
04G-4410
04G-4412
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
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aufInquire
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aufInquire
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aufInquire
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aufInquire
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04K-4348
04K-4405
04K-4406
04K-4407
04K-4409
04K-4408
04K-4411
04K-4413
04K-4414
04K-SO27
04K-4410
04K-4412
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
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aufInquire
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aufInquire
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aufInquire
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aufInquire
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ZT
ZT-SCX
ZT-WCX
ZT-WAX
04G-4426
04G-4466
04G-4478
04G-4463
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
04K-4426
04K-4466
04K-4478
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
ZTL
ZTL-SCX
ZTL-WAX
04G-4470
04G-4467
04G-4494
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
04K-4470
04K-4467
04K-4494
aufInquire
Anfrage
aufInquire
Anfrage
aufInquire
Anfrage
Sepra ZT
Sepra ZTL
Interested in MSPD for your analysis?

Please contact us for technique and
accessory information.
Florisil is a registered trademark of U.S. Silica Co.
* Larger quantities available upon request
352
Phenomenex
Accessories and
Consumables
General Laboratory Consumables

Autosampler Vials and Accessories .................................................. 21
Centrifugal Filters . .................................................................................. 12
Filter Membranes . .................................................................................. 15
Filtration System...................................................................................... 13
Syringe Filters................................................................................... 10-12
GC Accessories

Autosampler Vials and Accessories.................................................... 21
Capillary Unions and Splitters ............................................................... 99
Capillary Column Mini-Unions................................................................. 99
Ceramic Scoring Wafer (Capillary Cutter) ............................................ 114
Column Test Standards ......................................................................... 98
Cool-Lock Nut...................................................................................... 101
Ferrules . ...................................................................................... 102-103

Ferrule Remover Tool Kit ...................................................................... 114
FID Flame Detector Jet Cleaning Kit ................................................... 114
Gas Purifiers and Traps ........................................................................ 112
GC Fittings . ......................................................................................... 101
Injector Inlet Base Seals..................................................................... 100
Injector Inlet Septa . ............................................................................. 107
Injection Port Cleaning Kit .................................................................... 114
Injection Port Inlet Liners . ......................................................... 104-106
Installation Nuts .................................................................................... 101
Merlin Microseal Septum....................................................................... 113
Polyimide Resin ..................................................................................... 99
Sealing Rings......................................................................................... 107
Septa............................................................................................. 107, 113
Syringes ....................................................................................... 108-111
Test Mixes................................................................................................ 98
Vials and Accessories ......................................................................... 21
(Continued on next page)
ACCESSORIES
and Consumables
353 - 378
HPLC Accessories
Adapters................................................................................................ 360
Inlet Filters.............................................................................................. 16
Automated Fluidic Systems................................................................... 358
In-line Filters...................................................................................... 17-18
Autosampler Vials and Accessories.................................................... 21
Mixing Tee, PEEK................................................................................... 364
Backpressure Regulator........................................................................ 364
Mobile Phase Accessories........................................................... 13-16
Calibration Standards Kits..................................................................... 367
Mobile Phase Pickup Adapter................................................................. 14
Mobile Phase Reservoir and Reagent Bottles......................................... 14
Column Chiller / Heater......................................................................... 357
Mobile Phase Recycler.......................................................................... 356
Column Protection............................................................ 235, 355, 386
Polymer Calibration Standards.............................................................. 367
Column Heater..................................................................................... 357
Reducing Adapters................................................................................ 360
Column Sealing Plugs........................................................................... 363
Replacement Valve Caps......................................................................... 14
Column Selectors.................................................................................. 358
Rheodyne Fitting Wrench.............................................................. 373, 378
Column Test Standards.................................................................. 365-366
Sample Injectors............................................................................. 375-378
Connectivity Kit for UHPLC................................................................... 362
SecurityGuard Column Protection................................................. 235, 355
Couplers................................................................................................ 363
Solvent Reservoir and Reagent Bottles................................................... 14
Degasser................................................................................................ 359
Solvent Recycler.................................................................................... 356
Equipment.................................................................................... 356-359
Standards..................................................................................... 365-366
Filters.................................................................................................. 9-18
Sure-Fit Connector................................................................................ 364
Filter Cap................................................................................................. 14
Sure-Lok Coupler.................................................................................. 363
Filter Membranes..................................................................................... 15
Syringes........................................................................................ 368-370
Filtration Products.............................................................................. 9-18
Syringe Filters................................................................................... 10-12
Filtration System...................................................................................... 13
System Test Kit ..................................................................................... 355
Fingertight Fittings................................................................................. 363
Tubing .......................................................................................... 371-374
Fittings.......................................................................... 360-364, 371, 377
UHPLC Fittings............................................................................... 361-362
Fitting Tightening Tool........................................................................... 361
Unions............................................................................................ 361, 364
Flangeless Fittings................................................................................. 364
Valve Caps............................................................................................... 14
Fluid Processors.................................................................................... 358
Valves ........................................................................................... 375-378
Frits........................................................................................................ 374
Vials........................................................................................................ 21
Fused Silica Capillary Adapter............................................................... 360
Wrenches/Spanners............................................................................. 373
354
Phenomenex
Column Protection
Guard Columns
System Test Kit
Phenomenex recommends the use of SecurityGuard to protect all

your valuable HPLC columns from chemical garbage and damaging microparticulates. See page 235.
Analytical
For analytical separations the SecurityGuard innovative design provides a universal fit to virtually any HPLC column endfitting. Learn
more about this unique cartridge system and the many benefits
SecurityGuard gives you. See page 235. For ultra high pressure
applications, use SecurityGuard ULTRA. See page 239.
Diagnose hardware problems rapidly and easily

Avoid unnecessary and costly system repairs
Convenient benchmark testing of HPLC systems
using a C18 column standard
Test system setup and hardware connections
Quickly isolate method development problems
Kit Contents:
Phenomenex 5 m C18, 50 x 4.6 mm ID HPLC column
Five vials of Isocratic Test Mix containing the following
components in methanol/water (available separately as Part
No. CH0-1685):
Methylparaben
Ethylparaben
Propylparaben
Use with 2.0 to 8.0 mm ID Columns

Two cartridge sizes: 4.0 x 2.0 mm ID and 4.0 x 3.0 mm ID
SemiPrep
Butylparaben
Five vials of Gradient Test Mix containing the following
components in methanol/water (available separately as Part
No. CH0-1686):
Uracil
Ethylparaben
Phenol
Propylparaben
Methylparaben
Butylparaben
Use with 9 to 16 mm ID Columns

Cartridge size: 10 x 10 mm ID
PREP
System Test Kit
Part No.
Description
CH0-1684
HPLC System Test Kit, Reversed Phase,
includes: C18 column, isocratic and
gradient test mixes (5 each)
Isocratic Test Mix (5 vials)
Gradient Test Mix (5 vials)
Price
5/pk
5/pk
CH0-1685
CH0-1686
Unit
ea

Cartridge size: 15 x 21.2 mm ID
To protect your analytical column when an alkaline mobile phase

must be used, first pass the mobile phase through a silica presaturator column to saturate it with silica. Protect C18 columns
from acidic phases with a C18 presaturator column.
Presaturator Guard Columns
Part No.
Description
03A-0353-E0
Prepacked Silica Presaturator Column
03A-0355-E0
Prepacked C18 Presaturator Column
30 mm ID Guard Holder
Phenomenex

Cartridge size: 15 x 30.0 mm ID
Accessories and consumables
Presaturator Guard Columns

21.2 mm ID Guard Holder
COLUMN PROTECTION
10 mm ID Guard Holder
Price
For our line of HPLC Column Performance Check

Standards, see pp. 365-366
355
Equipment
Mobile Phase Recycler
SolventTrak
Saves up to 90 % of isocratic HPLC solvents
Peak detection algorithm accurately defines peaks and
diverts them automatically
Returns reliably clean solvent to mobile phase reservoir
SolventTrak Saves...
How Does SolventTrak Work?
Solvent purchases
The signal from any HPLC detector is connected to SolventTrak,

which monitors and continuously displays the voltage level on a
large, highly readable LCD panel located on the front of the instrument. The signal, if desired, can be connected directly to a chromatography data system or integrator.
Disposal costs
Preparation time
With the high cost of solvent and solvent disposal, a routine analytical HPLC system can recover the cost of SolventTrak in less than
45 days.
With a solvent recycler on board, youll save immediately and significantly in both time (watching and refilling solvent reservoirs) and
money (solvent purchase costs). And because the recycler dramatically cuts back on the total amount of hazardous wastes produced,
youll save money again in disposal costs, while conserving resources and protecting the environment.
SolventTrak peak detection sensitivity may be adjusted by touchsensitive controls which change familiar peak width and peak
threshold parameters, similar to those found on most integrators
and data systems.
Column
Data System/Integrator
Sample
Injector
What Does SolventTrak Do?
Equipment
Why is SolventTrak Reliably Accurate?

SolventTrak automatically detects eluting peaks and diverts them
to waste while sending the clean, uncontaminated solvent back to
the reservoir for recycling. Traditional recycling systems use a fixed
voltage level (or threshold above baseline) which must be exceeded
before peaks can be detected. If the baseline changes, new settings must be applied or contaminants may be recycled instead of
sent to waste. Conversely, pure solvent may be sent to waste rather
than being recycled
SolventTrak incorporates a unique integration algorithm to
accurately detect peaks in the eluent so that contaminants
are eliminated even if chromatography system conditions
cause the baseline to drift up or down
Solvents recycled with SolventTrak are cleaner and can be
used longer
30
Traditional recyclers
fail to detect small peaks

20
that are below the set
threshold level (i.e., 1 %)
1 % Threshold
SolventTrak
0
6
10
12
14
16
HPLC Pump
Waste
Mobile Phase
How Does SolventTrak Further Enhance

Purity?
When a peak is identified, an additional delay time may be set to
allow for volume between the detector and the valve. This further
enhances the accuracy and purity of the recycling. A LCD indicator
and a switchable audible alarm alert the operator that a peak has
been detected.
A reliable and inert electrically actuated solvent diverter valve automatically switches mobile phase to waste when peaks are detected.
The valve may also be intentionally switched via TTL or contact
closure by actuating that command on the front panel keypad if
and when required.
Accessories and Replacements Parts

100-312
SolventTrak Recycler CleanUp Kit, includes
Diverter Valve, Fittings, Mounting Bracket
100-314
SolventTrak Recycler Diverter Valve
Replacement (No Fittings or Bracket)
100-315
SolventTrak Recycler Fittings Kit, includes
10 ft. x 0.030 in. PTFE Tubing, Fittings, Ferrule
100-317
SolventTrak Recycler Mounting Bracket,
includes Screws
mV
1000
with its intelligent algorithm

accurately detects all peaks and
sweeps each entire peak to waste.
Solvent
Diverter
Valve
SolventTrak Mobile Phase Recycler
Part No.
Description
100-300
SolventTrak Mobile Phase Recycler
for Isocratic HPLC
Each SolventTrak is calibrated and certified to a NIST

traceable standard and arrives with a Certificate of
Validation
10
LCD
SolventTrak
SolventTrak, available through Phenomenex, is a solvent conservation system designed to recycle uncontaminated solvents used in
isocratic HPLC processes. In most systems, a high percentage of
the mobile phase can be recycled and re-used which saves money
by reducing solvent consumption and disposal costs.
Solvent Trak
LC Detector
18
min
Unit
ea
Price
ea
ea
ea
ea
Please specify Line Cord if other than North America

(Australia, Germany, Italy and UK are available).
SolventTrak is a trademark of Axxiom Chromatography, Inc.
356
Phenomenex
Equipment
Single-Column Heater
25C to 90C
Column Chiller/Heater
best seller
8C to 70C
ThermaSphere TS-130
ThermaSphere TS-430
Maintains the temperature of your HPLC column (and guard, if any)

at a precise degree set by user, thus improving reproducibility and
chromatographic results.
Improves reproducibility and chromatographic results
Essential for improving virtually all types (modes) of HPLC

separations.
Improves reproducibility and chromatographic results
Improves baseline and overall detector performance

Improves peak efficiency
Capacity to hold four (4) 30 cm columns up to 1 inch
outer diameter (up to 21.2 mm ID)
Improves baseline and overall detector performance

Open Position
for Column Access
Reduces analyte identification errors

Improves peak efficiency and analyte quantitation
(especially at low levels)
Improves the ruggedness of the separation (within-lab and
lab-to-lab)
Closed Position
Column heater in stand (optional), showing front control/display panel
Specifications
Specifications
Auto-Off Timer:
Injection Counter:
ThermaSphere TS-130 Column Heater
Part No.
Description
EH0-7057 ThermaSphere TS-130 HPLC Column Heater 25-90 C, 95
to 265 VAC, 50/60 Hz
EH0-7058 Stand for ThermaSphere TS-130 HPLC Column Heater
Electrical
Chiller/Heater:
Power consumption:
Electrical:
Peltier, 100 watts

230 watts AC
115 VAC, 50/60 Hz, 2.0 amp
230 VAC, 50/60 Hz, 1.0 amp
Dimensions
Chamber
Price
1. The ThermaSphere TS-130 is warranted for one year parts and

labor. Each unit is individually calibrated and comes with a
Certificate of Performance. No adjustment or re-calibration is
ever necessary. CE approved system, UL and CSA approved
power supplies.
2. Please specify Line Cord if other than North America (Australia,
Germany, Italy and U.K. are available)
If you are not completely satisfied with the
performance of your new TS-130 column heater,
simply return the unit within 60 days of purchase for
a full refund.
Phenomenex
8.0 C to 70.0 C
0.1 C
0.2 C
0.2 C
Yes
00:01 min/sec to 99:59 hrs/min
1 second
Yes (with Auto-off)
Overall Chassis
Valve hole diameter:
15.625 in. (39.69 cm) H x 6.385 in. (16.28 cm) W x 5.125 in.
(13.02 cm) D
25.5 in. (64.77 cm) H x 8.75 in. (22.23 cm) W x 3.25 in. (33.66 cm) D
1.625 in. (4.13 cm)
ThermaSphere Chiller/Heater Column Temperature Controllers
Part No.
Description
EH0-5720 ThermaSphere TS-430 Chiller/Heater HPLC Column
Temperature Controller, 8-70 C, 115 VAC, 50/60 Hz, 2.0 amp
EH0-5721 ThermaSphere TS-430 Chiller/Heater HPLC Column
Temperature Controller, 8-70 C, 230 VAC, 50/60 Hz, 1.0 amp
Over-temperature Alarm:
Temp. range:
Readability:
Accuracy:
Stability:
Convection fan:
Timer range:
Readability:
Audible alarm:
Temperature Range:
Temperature Stability:
Accuracy:
Power:
Fits up to one 30 cm length column, or 25 cm column with

guard column. Multiple inlet and outlet slots allow the shortest
length of tubing to be used with any length column.
From 25 to 90 C in 0.1 C increments.
0.1 C Calibration two-point, electronic, factory set.
0.5 C over the entire range.
12 volt DC universal power supply takes voltage inputs from 95
to 265 VAC, 50/60 Hz. CE approved.
Audible with automatic heater shutoff if column temperature
exceeds 10 C of target temperature.
Count down timer with audible alarm turns off heater, settable
to 30 days in days, hours, minutes and seconds.
Trigger on external switch closure.
Equipment
Column Size Accommodated:
Price
1. The TS-430 is warranted for 1 year parts and labor.

2. Please specify Line Cord if other than North America
(Australia, Germany, Italy and UK are available).
357
Equipment
Column Selectors
Fluid Processors
Synergi
Synergi
Quickly screen up to 6 HPLC columns

Develop, optimize and validate methods faster
Two-Position
Save time, boost productivity
Automates on-line sample enrichment, stripping, and

backflushing for decreased total analysis time
Speeds and automates routine work
Automates two-column selection
Easy, unattended operation
Automates multi-dimensional chromatography for

simultaneous sample clean-up and analysis
A few of the many applications you

can automate include:
Methods development and
column selectivity
Automates sample injection
Validation studies
Chiral analysis
2-D chromatography
Multi-user labs
The Synergi Column Selector can hold up to 6 analytical or
semi-prep columns (3-25 mm OD/5-35 cm L)
Equipment
Synergi Column Selectors
Part No.
Description
AV0-6080 Synergi 6-Column Selector
AV0-7241 Synergi Low-Profile 6-Column Selector
AV0-7039 Synergi Valve Repair Kit for 6-Column Selectors
Unit
ea
ea
ea
Price
All units supplied with 10-32 threaded PEEK nuts and ferrules
for all ports, 3.5 m (12 ft) of 1/16 in. OD PEEK tubing, power line
cord (please specify) and RS-232 interface cable. The selectors
are stainless steel and include 4 line BCD control, with port
for RS-232 communication (except the Low-Profile model),
Universal power supply 100-240 VAC, 50-60 Hz. Serial cable and
software not included. In order to control the Column Selector
via the ChemStation software, Agilent 1100 Systems require
the additional purchase (from Phenomenex or Agilent) of a BCD
Board (Agilent P/N G1351-68701) and External Contact Cable
(Agilent P/N G1103-61611).
Installation Kit
Part No.
Description
AV0-7136 Synergi Column Selector Installation Kit
The kit includes the following items:
Part No.
AT0-1260
AT0-1110
AQ0-1389
AQ0-1673
Description
PEEK Tubing 5 ft. x 1/16 in. OD x 0.007 in. ID, yellow
PEEK Tubing Cutter
Sure-Lok High Pressure Fitting, PEEK, 10-32 Threads
PEEK Zero Dead-Volume Union, 0.010 in.
thru-hole, with 2 fingertight fittings
Synergi 2-Position Fluid Processors
Part No.
Description
1 Line Level Logic Control
AV0-6082
2-Position, 6-Port Fluid Processor, SS
AV0-6083
2-Position, 10-Port Fluid Processor, SS
AV0-6084
2-Position, 6-Port Fluid Processor, PEEK
AV0-6085
2-Position, 10-Port Fluid Processor, PEEK
Synergi Valve Repair Kits
AV0-7041
Synergi Valve Repair Kit
for 2-position, 6-Port Fluid Processors
AV0-7042
for 2-position, 10-Port Fluid Processors
Unit
Price
ea
ea
ea
ea
ea
ea
Note: Please specify power line cord when ordering (see table at lower left).
Ten-Position
Accelerates and simplifies methods development
Automates selection of up to 10 samples for
precise injection
Eliminates the need to disconnect and reconnect fittings
Unit
ea
ea
ea
10/pk
ea
Price
Kit
Quantity
2
1
2
2
Synergi systems are warranted for one year parts and labor.
With the purchase of your new Synergi Column Selector, we

highly recommend you also get the installation kit, AV0-7136.
Frees up valuable research time
Synergi 10-Position Fluid Processors
Part No.
Description
4 Line BCD Control
AV0-6086
10-Position Fluid Processor, Stainless Steel
Synergi Valve Repair Kits
AV0-7045
for 10-position Fluid Processor
Unit
Price
ea
ea
Note: Please specify power line cord when ordering (see table at lower left).
Power Line Cords

(specify Part No., included at no charge)
North America
Schuko (most of Europe)
United Kingdom
358
AV0-6091
AV0-6092
AV0-6094
These kits contain all the parts necessary to refurbish the

corresponding Synergi valve. It is highly recommended that one
or more Valve Repair Kits be ordered at the same time as the
purchase of your Synergi system.
Phenomenex
Equipment
Solvent Degasser
best seller
Degassex
Improves chromatographic accuracy and precision
Degasses up to 4
individual solvents at once
Metal-free
(biocompatible)
Improves flow rate stability

Reduces detector noise level
Stabilizes baseline
Vacuum Degassing vs. Traditional Helium Degassing
The presence of dissolved oxygen in solvents has an adverse effect on the flow rate stability, noise level, and baseline stability of
HPLC systems. Degassing of mobile phases is an essential element
of reliable system performance. Degassing has traditionally been
performed by helium sparging or ultrasonication; both of these
methods are inefficient and time consuming, compared to vacuum
degassing.
Improved 4-Channel System
The Degassex DG-4400 is placed in line between the solvent reservoir and the pump(s). Solvent is drawn through the unit by the HPLC
pump and is degassed by vacuum filtration of dissolved gases
across a Teflon membrane. The efficiency exceeds that of all other
methods tested. The Degassex contains four solvent input channels, allowing degassing of up to four individual mobile phases. It
can be used with one HPLC system, or with multiple HPLC systems
in the same vicinity, or connected in series with one system for even
greater degassing efficiency.
Dissolved Oxygen (ppm)

Post Degassing
Improved
4-Channel System
4
3
2
1
0
(mL/min)
Water exposed to air at room temperature.

Degassex single-channel connection
10
Degassex DG-4400
Unit
ea
EH0-5847 4-Channel Degassex DG-4400
Solvent Degasser, 100 to 240 VAC, 50/60 Hz
Includes standard fittings, tubing and accessories:
4 ea of 1 m L x 1/8 in. OD x 1/16 in. ID PFA Teflon
Tubing
8 ea of PFA Teflon Fitting in.-28 threads for
1/8in. OD Tubing
8 ea of Tefzel Ferrule for 1/8 in. OD Tubing
1 Priming Syringe
1 Teflon Tube Adapter (connecting joint) for
Syringe
AQ0-5976 Waters Pump Connection Kit, includes:
kit
4 ea Flangeless Ferrule for 1/8 in. OD tubing,
Tefzel, Yellow
4 ea in.-28 Flangeless Nut for 1/8 in. OD tubing,
Teflon
4 ea Adapter in.-28 Female to 5/16 in.-24
Female, PEEK
4 ea Flangeless Ferrule for 3/16 in. OD tubing,
Tefzel
4 ea 5/16 in.-24 Peek Nut for 3/16 in. Flangeless
Fittings
1 ea FEP Teflon Tubing, 1/16 in. ID x 1/8 in. OD x
10ft. L
Accessories and Replacement Parts
AT0-2955 Standard Tubing 5 ft. L x 1/8 in. OD x 1/16 in. ID,
ea
PFA Teflon
AQ0-5849 Standard Flangeless Fitting and Ferrule
10/pk
for 1/8 in. OD tubing, in.-28 threads, PFA Teflon
AH0-5850 Priming Syringe
ea
AH0-5851 Teflon Tube Adapter (connecting joint) for Syringe
ea
AT0-5852 Waters Pump Tubing 10 ft. L x 3/16 in. OD x
ea
0.125 in. ID FEP Teflon
AQ0-5853 PEEK Flangeless Fitting 5/16 in.-24 threads and
10/pk
Tefzel Ferrule for 3/16 in. OD Tubing
AQ0-5854 Adapter 5/16 in.-24 Female and in.-28 Male adapter
ea
Price
Maximum degassing efficiency:

Maximum flow rate:
Pressure drop:
Flow path construction:
Dead volume:
Dimensions:
Weight:
Power:
4
CPU control and self-diagnostics
(with error output for quality system validation)
0.6 ppm at outlet for each channel
10 mL/min for each channel
0.06 kg/cm2 at 10 mL/min flow rate
Teflon and PEEK (metal-free)
approx. 7 mL per channel
71 W x 135 H x 310 D (mm)
5.1 kg
100 - 240 VAC (50/60 Hz)
Typical Degassing Performance, Dissolved Oxygen vs. Flow Rate
Number of channels:
System control:
Equipment
General Information
The metal-free Degassex DG-4400 is one of the most compact units
on the market and exhibits a very small pressure drop across the
inlet and outlet valves. With an integral universal power supply, the
unit will operate from a line voltage source of 100 to 240 VAC, 50 or
60 Hz, without selecting the line voltage. Degassex is an excellent
choice for effective and reliable in-line degassing of all your HPLC
solvents at a very economical price.
Specifications
Warranty includes one year parts and labor.
Phenomenex
359
Fittings
Fused Silica Capillary Adapter
Two reducing adapters for Phenomenex 50 mm ID Prep columns

allow smaller 1/16 in. ID system tubing to be used with the larger
1/8 in. ID column inlet end fittings, forming a positive leak-free seal
with zero dead volume. The smaller line from your system goes
directly into the adapter and the sample goes directly into the
column, without the short pieces of connecting tubing required if
a reducing union was used instead. Once the fitting is installed,
only one wrench is required to remove and reinstall it. Each end
of the column requires a fitting.
Fused Silica
Capillary Adapter
Fused Silica Capillary Adapter
Part No.
Description
AQ0-7833 Fused silica capillary connector for HPLC,
400 x 0.05 mm, self-assembled, includes fittings
Reducing Adapters
Unit
ea
Price
AQ0-7553 Reducing
Adapter for 50 mm
ID Axia Preparative
HPLC/SFC Hardware
Fused silica capillary adapter - connects capillary-LC columns

with Valco (Phenomenex) end-fittings directly to detectors that
require fused silica connections (can be cut to fit).
AQ0-7555 Reducing
Adapter for 50 mm
ID Traditional (Non-Axia)
HPLC/SFC Hardware
For other micro fittings, unions, and adapters, please contact

your Phenomenex technical consultant or distributor.
in.-28 to 10-32
Standard Adapter
Reducing Adapters
Part No.
Description
Complete Assembly
AQ0-7553
Reducing Adapter, 1/8 in. to 1/16 in. for 50 mm
ID Axia Preparative HPLC/SFC Hardware,
0.5 mm ID x 5/16 in. x 24 threads
AQ0-7555
Reducing Adapter, 1/8 in. to 1/16 in. for 50 mm
ID Traditional (Non-Axia) HPLC/SFC Hardware
Replacement Parts
AQ0-7554
1/8 in. Fittings for 50 mm ID Rounded Hardware,
2 Nuts and 2 Ferrules (Non-Axia Columns)
AQ0-3018
10-32 Threaded Male Nut and Ferrule Set for 1/16
in. OD capillary tubing
Make connections between different pieces

of liquid handling equipment
Made of sturdy and inert PEEK
Pressure rated to 1500 psi
Fittings
Simple to use fingertight design
Standard Adapter
Part No.
Description
AQ0-3351
in.-28 to 10-32 Standard Adapter, PEEK
Unit
ea
Unit
Price
2/pk
2/pk
2/pk
ea
Price
Maximum Temperature Ratings (C)

Material
PEEK
Delrin
Teflon (PFA)
Polyethylene (UHMW)
Polypropylene
KEL-F
Tefzel
Tubing
Fitting
100
N/A
80
N/A
N/A
N/A
80
150
60
80
50
40
80
80
For "Selecting the Right Tubing and Fittings", see p. 371
360
Phenomenex
Fittings
Ultra-High Performance LC Fittings
UHPLC / HPLC Sure-Lok High Pressure
PEEK Male Nut Fittings
Ultra-High Performance LC/HPLC

Stainless Steel Nut and Ferrule Set
Pressure rated to 12,000 psi (827 bar)
For the ultra-high pressure connections use this specially-designed

10-32 stainless steel nut and ferrule set. The metal ferrule cuts a
ring near the end of the tube to swage the fitting to the tube, and will
provide a maximum operational limit of 28,000 psi (1,930 bar). Seating (swaging) the fitting usually takes only about a turn beyond
the point where the ferrule first starts to grab the tubing.
Stable up to temperatures of 200C

Made of a proprietary PEEK blend, these ultra-high performance
polymeric fittings are perfect for all but the most extreme high-pressure applications, and best for ion- and bio-chromatography. High
pressure nuts have a knurled surface designed to provide sufficient
sealing force on the ferrule without wrenches. For 1/16 in. diameter
tubing, there are two design types. The convenient one-piece design is pressure rated on S.S. tubing to 12,000 psi (827 bar) and
stable up to temperatures of 200 C. The second type is engineered
as a 3-piece unit, with a ferrule and stainless steel gripping ring, that
will provide leak-free connections up to 19,000 psi (1,310 bar), on
S.S. tubing. Upper pressure limits of these fittings when used with
polymeric tubing (such as PEEK) depends on the pressure rating
of the tubing. Phenomenex PEEK tubing is rated to 7,000 psi (482
bar). For higher pressure-rated fittings use the stainless steel nut
and ferrule set (AQ0-8506).
AQ0-8503
Pressure rated up to
12,000 psi (827 bar)
AQ0-8504 shown
with AQ0-8505
Pressure rated to
19,000 psi (1,310 bar)
Unit
2/pk
Price
Unit
2/pk
Price
10/pk
Important: To achieve the maximum pressure rating, 45 lbs

of torque is required.
Ultra-High Performance LC/HPLC

Stainless Steel Zero Dead-Volume Union
Stainless steel construction
10/pk
FITTINGS
For 1/16 in. OD tubing, with 10-32 threading
10/pk
0.010 in. thru hole, 20 nL swept volume
10/pk
Includes 2 fittings (nuts and ferrules)
Sure-Lok High Pressure PEEK Nuts
Part No.
Description
AQ0-8502
Sure-Lok High Pressure PEEK 1-Pc Nut, 10-32,
for 1/16 in. Tubing, 12,000 psi (827 bar)
AQ0-8503
Sure-Lok High Pressure PEEK 1-Pc Nut, 10-32,
for 1/16 in. Tubing, 12,000 psi (827 bar)**
AQ0-8504
Sure-Lok High Pressure PEEK Nut, 10-32,
for 1/16 in. Tubing, 19,000 psi (1,310 bar) *,**
AQ0-8505
Sure-Lok PEEK Ferrule Assembly (2-pc),
for High Pressure 2-Pc Nut (AQ0-8504)
Nut and Ferrule Set (Stainless Steel)
Part No.
Description
AQ0-8521
Nut and Ferrule Set, SS, 10-32, for 1/16 in. Tubing,
28,000 psi (1,930 bar)
AQ0-8506
Nut and Ferrule Set, SS, 10-32, for 1/16 in. Tubing,
28,000 psi (1,930 bar)
** Sure-Lok fitting tightening tool is required for AQ0-8503 and AQ0-8504
Recommended for PEEKsil tubing applications.
Sure-Lok Fitting Tightening Tool

Use this handy tool to tighten any standard, short- or long-style
knurl-headed (high pressure) male nut like the ones above. The
tool can also be used with many of the low-pressure nuts commonly used in the lab.
AQ0-8530
Sure-Lok Fitting
Tightening Tool
AQ0-8502 or
AQ0-8503
Sure-Lok HIGH
Pressure PEEK
1-pc Fitting
(12,000 psi/827 bar)
AF0-8497
KrudKatcher
Ultra
Column In-Line
Filter
Sure-Lok Fitting Tightening Tool
Part No.
Description
AQ0-8530
Sure-Lok Fitting Tightening Tool, Aluminum
Phenomenex
Zero Dead-Volume Union (Stainless Steel)
Part No.
Description
AQ0-8507
Zero Dead-Volume Union, SS, with Fittings,
10-32, for 1/16 in. Tubing, 28,000 psi (1,930 bar)
Unit
ea
Price
* Ferrule assembly (AQ0-8505) must be ordered separately.
HPLC Column
Unit
ea
Price
361
Fittings
n
e
w
Connectivity Kit for UHPLC

Optimize UHPLC system connections for both routine and
critical applications
Increase method efficiency, resolution, and detection
Minimize dead volume between injector and detector
Make connections quickly and reliably every time, on any
analytical system
Improve results for sensitive and demanding applications
The connections made throughout the system are critical to
maximizing the benefit from your UHPLC setup. The fittings and
tubing used in this kit are carefully chosen to minimize dead volume
and reduce band broadening. Combined with your Kinetex column
and SecurityGuard Ultra column protection system, the kit will
provide reliable connections and quality performance every time.
Connectivity Kit for UHPLC
Part No.
Description
AQ0-8892 UHPLC Connections Kit, Includes: PEEKsil Tubing, Fittings and Tool*
Price
Kit Quantity
2/pk
ea
10/pk
ea
Unit
5/pk
5/pk
10/pk
ea
Price
For more information on Part No. AT0-8896 and AT0-8897 see p. 372.
For more information on Part No. AQ0-8503 and AQ0-8530 see p. 361.
Fittings
*UHPLC Connections Kit AQ0-8892 includes the following components:

PEEKsil Tubing 0.100 mm ID x 1/16 in. OD x 20 cm L, Red
PEEKsil Tubing 0.100 mm ID x 1/16 in. OD x 10 cm L, Red
Sure-Lok High Pressure PEEK 1-Pc Nut, 10-32, for 1/16 in. Tubing
Sure-Lok Fitting Tightening Tool, Aluminum
Accessories and Replacement Parts
Part No.
Description
AT0-8896 PEEKsil Tubing 0.100 mm ID x 1/16 in. OD x 20 cm L, Red
AQ0-8503 Sure-Lok High Pressure PEEK 1-Pc Nut, 10-32, for 1/16 in. Tubing
Unit
ea
CAUTION: PEEKsil tubing is pressure-rated to

10,000 psi (827 bar)
For more in-depth tips and hints on optimizing

your UHPLC, visit www.phenomenex.com
362
Phenomenex
Fittings
Sure-Lok Fingertight Male
Nut Fittings
Analytical Column Couplers
Fingertight to 5,000 psi (345 bar)
Universal and reusable
Compatible with all 10-32 HPLC fittings
Solvent resistant material
Polymer construction compatible with nearly all HPLC

and GPC solvents
Low dead-volume connection
Sure-Lok Coupler
Compatible with all 10-32 internal-threaded fittings

Applications:
Filter to column
Column to column
Precolumn to column
Column to detector
Sure-Lok Fingertight Male Nut (PEEK)

Sure-Lok Fingertight Male Nuts
Part No.
Description
AQ0-1388
PEEK Sure-Lok Fingertight Male Nut
AQ0-1389
PEEK Sure-Lok Fingertight Male Nut
Unit
ea
10/pk
Price
Sure-Lok Coupler (PEEK)
Sure-Lok Couplers contain two Sure-Lok male nuts at either end of

a 5 cm long 1/16 in. tubing. The PEEK biocompatible coupler has all
parts composed of PEEK, including the 0.010 in. ID tubing. Fingertight to 5,000 psi (345 bar).
Sure-Lok Couplers
Part No.
Description
AQ0-1392
AQ0-1393
Nut and Ferrule Plugs

Wrench tight to 10,000 psi (690 bar)
Unit
ea
10/pk
Price
Unit
ea
Price
Unit
ea
Price
Column Coupler
Don't let resolution be a limiting factor!
Couple several columns together
Nut and Ferrule
Maintain separation efficiency

No influence on backpressure
Unit
ea
FITTINGS
Nut and Ferrule
Part No.
Description
AQ0-3018
10-32 Threaded Male Nut and Ferrule Set for
1/16 in. OD capillary tubing
Price
Seal column for storage

Column Coupler
Part No.
Description
AQ0-7654
Onyx Column Coupler
10-32 threads fit most columns
PREP Column Coupler
Part No.
Description
AQ0-0217
Column Sealing Plug, 10-32 thread size
Phenomenex
Unit
10/pk
Price
PREP Column Coupler
Part No.
Description
AQ0-8374
PREP Coupler, Stainless Steel with PEEK
Ferrule Inserts, 1/16 in. OD x 0.020 in. ID
AQ0-8375
Replacement Ferrule Inserts for PREP Coupler,
PEEK 0.020 in. ID
10/pk
363
Fittings
Sure-Fit Connector
14 in.-28 Flangeless Fittings
Self-adjusting to any port depth regardless

of manufacturer, with virtually no dead volume
For use with 1/16 in. or 1/8 in. polymeric tubing
Available in PEEK for biocompatibility
Replaces Cheminert and Omnifit fittings
Fingertight to 6000 psi (414 bar)
Pressure rated to 1400 psi (97 bar)
Easy 2-piece design
No twisting or bending of tubing
Unit
Price
2/pk
2/pk
ea
ea
2/pk
2/pk
Unit
10/pk
Price
10/pk
ea
ea
ea
Backpressure Regulator
10/pk
Adjustable with preset pressure ratings
10/pk
Consistent backpressure at various flow rates

Flow-through, low-volume design (146 mL)
These connectors are also available in kits for use with specific
instruments. Call your Phenomenex Technical Consultant.
Fittings
Sure-Fit Connectors
Part No.
Description
Sure-Fit Single-Ended Connector
AQ0-5725 Stainless Steel Connectors, 0.007 in. ID x 10 cm L
AQ0-7051 PEEK Connectors, 0.007 in. ID x 10 cm L
AQ0-7052 PEEK Connectors, 0.007 in. ID x 20 cm L
Sure-Fit Double-Ended Connector
AQ0-5981 Sure-Fit Connector replacement ferrules
(for stainless steel version)
AQ0-7053 Sure-Fit Connector replacement ferrules
(for PEEK version)
Flangeless Fittings
AQ0-2949 Flangeless Nut and Ferrule for 1/16 in. tubing,
in.-28 threads, red Delrin
AQ0-2950 Flangeless Nut and Ferrule for 1/8 in. tubing,
in.-28 threads, green Delrin
AT0-2951 Teflon Tubing, 5 ft. L x 1/16 in. OD x 0.010 in. ID
10-32 PEEK Mixing Tee
Backpressure Regulators
Part No.
Description
AQ0-0222
40 psi Backpressure Regulator
AQ0-0223
AQ0-0224
Unit
ea
ea
ea
Price
PEEK Zero
Dead-Volume Union
Use with 1/16 in. OD polymeric or metal tubing

Mixing tee for pre- or post-column derivatization
Chemically inert and fully biocompatible
Simple-to-use fingertight design
Zero dead-volume connection, 0.010 in. thru-hole
Pressure rated to 4000 psi (276 bar)
Wrench/fingertight fittings
PEEK Mixing Tee
Part No.
Description
AQ0-2002
PEEK Tee, 0.020 in. thru-hole*
Unit
ea
*Fittings not included, use PEEK Sure-Lok fingertights part no. AQ0-1389, see p.363
Price
PEEK Zero Dead-Volume Union
Part No.
Description
AQ0-1673
PEEK Zero Dead-Volume Union, 0.010 in. thruhole, with 2 fingertight fittings
Unit
ea
Price
For UHPLC Stainless Steel Zero Dead-Volume Union, see p. 361
364
Phenomenex
Standards
HPLC Column Check Standards
* For Ultracarb C8, ODS(20), ODS(30) inject 1.5 L for all column
sizes.
Reversed Phase 2
App ID 15640
Unit quantity:
Contains:
Diluent:
Test Conditions
Mobile Phase:
Flow Rate:
Injection Volume:
Detection:
Price:
2 mL
Meta-xylene; Nitrobenzene
Hexane/Acetonitrile (99:1)
(For Onyx C8, C18, and HD-C18)
Price:
Unit quantity: 2 mL
Contains: Thiourea 10 g/mL; Progesterone 100
g/mL; Anthracene 10 g/mL
Diluent: Acetonitrile/Water (60:40)
Test Conditions
Flow Rate: 2.0 mL/min*
Injection Volume: 1.0 L
Storage
Conditions: Refrigerate @ 4 C
Onyx Monolithic Normal Phase
(For PolySep GFC-P and other aqueous-soluble analysis

columns)
Part No.: AL0-3043
Price:
2 mL
Ethylene Glycol
Water
Water
0.8 mL/min
3.0 L
RI
(For Onyx Si (Silica))
Part No.: AL0-7835
Price:
Unit quantity: 2 mL
Contains: Toluene 21.75 g/mL; Nitrobenzene
150 g/mL; 2-Nitroanisol 0.18 g/mL
Diluent: n-heptane/Dioxane (95:5)
Test Conditions
Mobile Phase: n-heptane/Dioxane (95:5)
Storage
App ID 14759
App ID 15639
Aqueous SEC 2
Unit quantity:
Contains:
Diluent:
Test Conditions
Mobile Phase:
Flow Rate:
Injection Volume:
Detection:
Hexane/Acetonitrile (99:1)
1.0 mL/min
1.0 L
UV @ 254 nm
Price:
Unit quantity: Dry; Reconstituted to 2 mL

Contains: Bovine thyroglobulin; Human gamma
globulin (contains IgA and IgG);
Ovalbumin; Myoglobin; Uridine
(reconstitute with 1 mL of 100 mM
Sodium Phosphate pH 6.8)
Diluent: 100 mM Sodium phosphate, pH 6.8
Storage: Add 0.1 % NaN3 to the solution and
refrigerate
Test Conditions
Mobile Phase: 100 mM Sodium phosphate, pH 6.8
Flow Rate: 1.0 mL/min for a 300 x 7.8 mm column
STAR-ION A300
Part No.: AL0-3420
Test Conditions
Mobile Phase:
Flow Rate:
Injection Volume:
Detection:
* For a 100 x 4.6 mm column
HILIC Phase
Price:
Unit quantity: 2 mL
Contains: Fluoride (5 mg/mL); Chloride (10 mg/
mL); Nitrite (20 mg/mL); Bromide (20
mg/mL); Nitrate (20 mg/mL); Phosphate
(30 mg/mL); Sulfate (20 mg/mL)
Diluent: 1.7 mM NaHC03/1.8 mM Na2C03
1.7 mM NaHC03/1.8 mM Na2C03
1.5 mL/min for a 100 x 4.6 mm column
20 L
(For Luna HILIC; Kinetex HILIC )
Part No.: AL0-8317
Price:
Unit quantity: 2 mL
Contains: Toluene; Uracil; Cytosine
Diluent: Acetonitrile
(containing toluene) / Water, no buffer
(85:15)
Test Conditions
Mobile Phase: Acetonitrile/100 mM Ammonium
Formate, pH 3.2 (90:10)
PolymerX RP-1
Part No.: AL0-7260
Price:
Unit quantity: 2 mL
Contains: Cytosine 13 mg/mL; Uracil 13 mg/mL;
Uridine 33 mg/mL
Diluent: Water
Test Conditions
Mobile Phase: 0.05 M Citric Acid, pH 4.2
Storage
Onyx Monolithic Reversed Phase
Phenomenex
(For Si, NH2, N02, Diol, Alumina, PAC and Luna CN)
Part No.: AL0-3033
(For BioSep-SEC-S and other protein SEC columns)
Part No.: AL0-3042
* Columns with dimensions of 50 x 2.0 mm, 30 x 2.0 or

1.0 mm, the mobile phase ratio should be 50:50. Some 50 x
2.0 mm columns use 65:35.
Normal Phase
Price:
Unit quantity: 2 mL
Contains: Uracil; Acetophenone; Toluene;
Naphthalene
Test Conditions
For Jupiter C18, all Luna (except CN), Aqua, Synergi, Gemini,
Prodigy, Clarity Oligo-RP and Columbus columns
Mobile Phase: Acetonitrile/Water (65:35)*
Flow Rate: 1.0 mL/min; 0.75 mL/min for 3 m
particles
Test Conditions
For Jupiter C4 and C5 columns
Part No.: AL0-7836
Unit quantity: 2 mL
Contains: Uracil; Acetophenone; Toluene;
Naphthalene; Acenaphthalene (2.5
mg/mL)
Diluent: Acetonitrile/Water (50:50)
Test Conditions
(For Prodigy C8, ODS(2), ODS(3); Luna C5, C8, C18, PFP(2),
Phenyl-Hexyl; Jupiter C4, C5, C18; Jupiter Proteo;
Columbus C8, C18; Aqua; PhenoSphere-NEXT C8, C18;
Synergi; Gemini C18, C6-Phenyl; Gemini-NX C18; Clarity
Oligo-RP; Kinetex C8, C18, XB-C18, PFP, Phenyl-Hexyl; 4.6
mm ID Aeris WIDEPORE XB-C18, XB-C8, XB-C8, C4; Aeris
PEPTIDE XB-C18)
Part No.: AL0-3045
Price:
Aqueous SEC 1
HPLC Column Check Standards
App ID 14754
best seller
Part No.: AL0-8931
App ID 14752
Unit quantity: 2 mL
Contains: Uracil; Benzamide; Benzophenone;
Biphenyl (refer to product insert for
specific details)
Test Conditions
Mobile Phase: Acetonitrile/Water (percentages
depend on phase)
Injection Volume: 1.0 L for 3 and 5 m particles*
1.5 L for 10 m particles
(For 2.1 mm ID Aeris WIDEPORE XB-C18, XB-C8, C4)
App ID 14753
Price:
Aeris Narrow ID
App ID 14760
Part No.: AL0-3034
App ID 19812
(For C1, C18, CN and Phenyl)
App ID 14743
Reversed Phase 1
App ID 16399
App ID 14744
We recommend using check standards to verify performance of

all new columns and periodically over their lifetime. Standards for
all Phenomenex brand name columns are available. Standards are
grouped by column type (e.g. normal phase standard for Si, -NH2,
-Diol, -NO2, alumina and PAC).
* For a 5 m 250 x 4.6 mm column
Flow rates and Injection volumes are

for 250 x 4.6 mm size columns, unless
otherwise noted.
HPLC Column Check Standards ordering

information continues on next page
365
Standards
Price:
Chiral Test Mix No. 2

(Applicable to the following Chirex columns)
Part No.: AL0-3047
Price:
Price:

(Applicable to the following Lux columns)
Part No.: AL0-8412
Price:
Description
Cellulose Tris (3,5-Dimethylphenylcarbamate)
Lux Cellulose-2 Cellulose Tris (3-Chloro4-methylphenylcarbamate)
Lux Cellulose-3 Cellulose Tris (4-Methyl-benzoate)
Lux Cellulose-4 Cellulose Tris (4-Chloro3-methylphenylcarbamate)
Lux Amylose-2 Amylose Tris (5-Chloro2-methylphenylcarbamate)
Unit quantity: 2 mL
Contains: Trans-Stilbene oxide, 0.5 mg/mL,
CAS [1439-07-2]
Diluent: Hexane/Isopropanol (90:10)
Test Conditions
Mobile Phase: Hexane/Isopropanol (90:10)
Chirex Phase
3014
3017
3018
3019
Phase Description
Bond Type
(S)-Valine and (R)-1--NEA Covalent
(S)-Proline and (S)-1--NEA Covalent
(S)-Proline and (R)-1--NEA Covalent
(S)-tert-Leucine and (S)-1Covalent
-NEA
3020
(S)-tert-Leucine and (R)Covalent
1--NEA
3022
(S)-Indoline carboxylic acid Covalent
and (R)-1--NEA
(NEA = Naphthylethylamine)
Unit quantity: 2 mL
Contains: (+/-)-N-(3,5-Dinitrobenzoyl)-methylbenzylamine CAS
[69632-32-2] and [69632-31-1]
Diluent: Hexane/Isopropanol/Ethanol
(70:20:10)
Test Conditions
Mobile Phase: Hexane/Isopropanol/Ethanol
(70:20:10)
For GC Column Performance Check

For Polymer Calibration Standards,
see p. 367
Flow rates and Injection volumes are
for 250 x 4.6 mm size columns, unless
otherwise noted.
Carbohydrate Mix 1
(For Rezex RNM, RAM & other carbohydrate analysis
columns)
Part No.: AL0-3035

Unit quantity:
Contains:
Diluent:
Test Conditions
Mobile Phase:
Flow Rate:
Temperature:
Injection Volume:
Detection:
2 mL
Maltotriose Hydrate; Maltose; Ribitol
Water
Water
85 C
5.0 L
RI
Carbohydrate Mix 2
(For Rezex RPM & other carbohydrate analysis columns)
Part No.: AL0-3036

Unit quantity:
Contains:
Diluent:
Test Conditions
Mobile Phase:
Flow Rate:
Temperature:
Injection Volume:
Detection:
(For Rezex RCM, RCU & other carbohydrate analysis columns)
Part No.: AL0-3037
Price:
Unit quantity: 2 mL
Contains: Melezitose; Maltose; Glucose; Mannose;
Fructose; Ribitol
Diluent: Water
Test Conditions
Mobile Phase: Water
Flow Rate: 0.6 mL/min for a 300 x 7.8 mm column
Temperature: 85 C
Detection: RI
Oligosaccharide Standard
(For Rezex RSO, RNO & other oligosaccharide analysis
columns)
Part No.: AL0-3038
Price:
2 mL
Light corn syrup
Water
Water
0.3 mL/min for a 200 x 10 mm column
85 C
5.0 L
RI
Organic Acid Standard

(For Rezex ROA & other organic acid analysis)
Part No.: AL0-3039
Price:
Unit quantity: 2 mL
Contains: Oxalic acid; Citric acid; Tartaric acid;
Succinic acid; Formic acid; Acetic acid
Diluent: 5 mM Sulfuric Acid
Test Conditions*
Mobile Phase: 0.005N H2S04
Temperature: 85 C
Price:
App ID 14750
Price:
Cation-Exchange
(For SCX, SA, CM)
Part No.: AL0-3040

Unit quantity:
Contains:
Diluent:
Test Conditions
Mobile Phase:
Flow Rate:
Injection Volume:
Detection:
Price:
2 mL
Uracil; Cytosine
Water
0.15 M (NH4)2 HPO4 , pH 6.0
1.0 mL/min
1.0 L
UV @ 254 nm
Price:
2 mL
Melezitose; Glucose; Fructose; Ribitol
Water
Water
85 C
1.0 L
RI
App ID 14751
App ID 14745

Part No.: AL0-3048
Carbohydrate Mix 3
Unit quantity:
Contains:
Diluent:
Test Conditions
Mobile Phase:
Flow Rate:
Temperature:
Injection Volume:
Detection:
Phase
Lux Cellulose-1
App ID 14746
App ID 14757
Phase Description
Bond Type
(S)-Valine and DNAn
Covalent
(S)-Leucine and DNAn
Covalent
(R)-Phenylglycine and DNAn Covalent
(DNAn = 3,5-Dinitroaniline
Unit quantity: 2 mL
Contains: N-dansyl-DL-valine
(cyclohexylammonium salt);
CAS[84540-67-0]
Diluent: 10 mM ammonium acetate in
methanol
Test Conditions
Mobile Phase: 10 mM ammonium acetate in
methanol
| STANDARDS
Chirex Phase
3010
3011
3012
366
Part No.: AL0-3049
Chirex Phase Phase Description

Bond Type
3126
N,S-dioctyl-(D)Ionic
Penicillamine
Unit quantity: 2 mL
Contains: DL-Aspartic Acid CAS [617-45-8]
Diluent: 2 mM Copper sulfate pentahydrate
in water/Isopropanol (95:5)
Test Conditions
Mobile Phase: 2 mM Copper sulfate
pentahydrate in
water/Isopropanol (95:5)
App ID 17476
App ID 14756
Chirex Phase Phase Description

Bond Type
3001
(R)-Phenylglycine-DNB
Covalent
3005
(R)-Naphthylglycine and DNB Covalent
Unit quantity: 2 mL
Contains: (+/-)-2,2,2-trifluoro-1-(9-anthryl)
ethanol CAS [60646-30-2] and
[53531-34-3]
Diluent: Hexane/Ethanol (95:5)
Test Conditions
Mobile Phase: Hexane/Ethanol (95:5)
App ID 14747
Part No.: AL0-3046
App ID 14748
App ID 14749
App ID 14758
App ID 14755
HPLC Column Check Standards (contd)
Anion-Exchange
(For SAX, SB, DEAE, PEI)
Part No.: AL0-3041
Price:
Unit quantity: 2 mL
Contains: Uridine, UMP (refer to product insert for
specific details)
Diluent: Water
Test Conditions
Mobile Phase: 0.15 M (NH4)2 HPO4 , pH 6.0
Phenomenex
Standards
Polymer Calibration
Standards Kits
Aqueous and organic standards
Low, medium, high, and ultra-high
molecular weights available
Narrow, medium and broad molecular
weight distribution
Organic SEC (GPC) Calibration Kits

All calibration kits contain standards that are uniformly separated
logarithmically to give equally distributed data points on a
calibration curve over the Mp (peak molecular weight) range
stated.
Polystyrenes
Part No.
MW
AL0-2761
Low MW Narrow MWD Kit
AL0-2762
Medium MW Narrow MWD Kit
AL0-2763
High MWD Narrow MWD Kit
Kit Description
8 Standards
6 Standards
6 Standards
Mp Range
100-10,000
10,000-100,000
120,000-1,850,000
Unit
250 mg/standard
250 mg/standard
250 mg/standard
Price
For GPC columns, see p. 216
For HPLC Column Check Standards, see pp. 365-366

For GC Column Check Standards, see p. 98
STANDARDS
Aqueous SEC (GFC) Calibration Kits

All calibration kits contain standards that are uniformly separated
logarithmically to give equally distributed data points on a
calibration curve over the Mp (peak molecular weight) range
stated.
Kit Description
7 Standards
8 Standards
Mp Range
200-50,000
7,200-1,580,000
Unit
250 mg/standard
1.0 g/standard
Price
Polyethylene Glycols
Part No.
MW
AL0-2774
Narrow MWD Kit
Kit Description
10 Standards
Mp Range
100-22,500
Unit
1.0 g/standard
Price
Polystyrene Sulfonates
Part No.
MW
AL0-4423
Broad MWD Kit
Kit Description
10 Standards
Mp Range
1,000-2,000,000
Unit
250 mg/standard
Price
Polyethylene Oxides
Part No.
MW
AL0-2775
Narrow MWD Kit
Kit Description
6 Standards
Mp Range
20,000-1,000,000
Unit
250 mg/standard
Price
Phenomenex
Dextrans (Polysaccharides)
Part No.
MW
AL0-2771
Branched, Kit
AL0-2772
Branched, Broad MWD Kit
367
Syringes
Hamilton HPLC Injector Syringes
Hamilton Gastight Syringes
For Rheodyne, Altex and Valco (VISF-2) Injectors
best seller
Needle Dimensions: 22, 22s Gauge (.028 in. OD); Point Style #3; 2 in. Long
Rheodyne, Altex and Valco (VISF-2) Injector Syringes
Part No.
Mfr. No.
Model No.
Capacity (L)
AS0-3353
87943
65 RNR
5
AS0-0022
80365
701 SNR
10
AS0-0023
80465
702 SNR
25
AS0-0024
80565
705 SNR
50
AS0-0025
80665
710 SNR
100
AS0-0026
80765
725 SNR
250
AS0-0027
80865
750 SNR
500
Unit
ea
ea
ea
ea
ea
ea
ea
Price
1000 Series Gastight Syringes

The Hamilton 1000 Series GASTIGHT syringes with Teflon Luer lock tip are available
in volumes from 1.0 to 100 mL (Luer lock needle not included).
Part No.
Mfr. No.
Model No.
Capacity (mL)
Unit
Price
AS0-1907
81320
1001 TLL
1.0
ea
AS0-1908
81420
1002 TLL
2.5
ea
AS0-1909
81520
1005 TLL
5.0
ea
AS0-1910
81620
1010 TLL
10.0
ea
Luer Lock Needles with Fluorocarbon Hub, 2 in. long, #2 pt style
AS0-1914
90126
KF 726
26 gauge
6/pk
AS0-1915
90122
KF 722
22 gauge
6/pk
AS0-1916
90116
KF 716
16 gauge
6/pk
| Syringes
For Waters U6K Injector
Needle Dimensions: 25s Gauge; Point Style #3; 1.967 in. Long
Waters U6K Injector Syringes
Part No.
Mfr. No.
AS0-0028
84815
AS0-0029
84816
AS0-0030
84817
AS0-0031
84818
Model No.
801 RNW
802 RNW
805 RNW
810 RNW
Capacity (L)
10
25
50
100
Unit
ea
ea
ea
ea
Price
Needle point #2 (22 bevel) is used for GC and

most applications which require the puncturing
of a septum.
Needle point style #3 (90 ) is appropriate for
HPLC applications.
1700 Series Gastight Syringes

The Hamilton 1700 Series GASTIGHT syringes are available in volumes from 10-500 L.
The 1700 series GASTIGHT syringe with removable needle in either point style #2
(22 bevel) or #3 (90) is a universally popular style for most GC and HPLC applications.
Capacity
Part No.
Mfr. No.
Description*
(L)
Unit
Price
Needle Point Style #2
AS0-1898
80030
1701RN (26s/2 in./2)
10
ea
AS0-1899
80230
1702RN (22s/2 in./2)
25
ea
AS0-1900
80930
1705RN (22s/2 in./2)
50
ea
AS0-1901
81030
1710RN (22s/2 in./2)
100
ea
AS0-1902
81130
1725RN (22s/2 in./2)
250
ea
AS0-1903
81230
1750RN (22s/2 in./2)
500
ea
Needle Point Style #3
AS0-4380
80065
1701RN (22s/2 in./3)
10
ea
AS0-4381
80265
1702RN (22s/2 in./3)
25
ea
AS0-4382
80965
1705RN (22s/2 in./3)
50
ea
AS0-4383
81065
1710RN (22s/2 in./3)
100
ea
AS0-4384
81165
1725RN (22s/2 in./3)
250
ea
AS0-4385
81265
1750RN (22/2 in./3)
500
ea
The Hamilton 1700 Series syringes are also available with Teflon Luer lock tip, from 50 to
500 L sizes.
* RN = Removable Needle. Values in parentheses denote the following: (Gauge/Length/Point Style).
"s" after Gauge denotes smaller/reduced internal needle diameter.
Cemented Needle
Removable Needle
Syringes continued on next page.
368
Phenomenex
Syringes
Hamilton 10 mL Gastight
Priming Syringe
VICI Series A-2 Gas Syringes
For Waters HPLC Pumps

(Models 6000, 6000A, 501, 510, 610 and 610E)
Pressure-Lok design offers gas-tight injections
Push-button valve and smaller volumes

Inject without carrier backflushing to sharpen peaks
and improve separations
Leak-tight to 250 psi - liquids and gases
No plunger "blow out" at elevated pressures
The A-2 features a push-button valve, which allows sample storage
up to 250 psi in syringes as small as 25 L. This is useful with small
liquid samples containing low-boiling components that would be
lost through evaporation using ordinary syringes.
Hamilton 10 mL Gastight Priming Syringe
Part No.
Mfr. No.
Model No. Capacity (mL)
AS0-1906
81610
1010W
10
Unit
ea
Vol. Range
2.5-100 L
25-100 L
25-100 L
250 L-10 mL
250 L-10 mL
2.5-100 L
2.5-100 L
250 L-10 mL
250 L-10 mL
250 L-10 mL
2.5-100 L
Price
Unit
6/pk
6/pk
6/pk
6/pk
6/pk
6/pk
6/pk
6/pk
6/pk
6/pk
6/pk
Price
VICI Precision Sampling Syringes
Part No.
AS0-4739 050023 Series A-2 Syringe
Capacity (L)
25
50
100
250
500
1000 (1 mL)
2000 (2 mL)
5000 (5 mL)
Unit
ea
ea
ea
ea
ea
ea
ea
ea
Notes: (1) All other VICI Precision Sampling products not listed are available.
(2) Removable needle: 0.028 in. x 0.005 in. x 2.00 in., bevel, open end on 25, 50 and
100 L (p/n 943050, 3/pk); 0.029 in. x 0.012 in. x 2.00 in., bevel, open end on all others
(p/n 943051, 3/pk).
for Rheodyne Injectors

for Waters U6K Injectors
3
for Agilent 7673 Autosampler
* RN = Removable Needle. Values in parentheses denote the following: (Gauge/Length/Point Style).
"s" after Gauge denotes smaller/reduced internal needle diameter.

Use for all syringe filter applications
Luer-lock outlet makes connection easy
Capacities ranging from 3 to 20 mL
Made of ultra-clean, high-purity plastics
Part No.
Description
Capacity (mL)*
3
5
10
20
Unit
100/pk
100/pk
100/pk
100/pk
Price
Syringes
Removable Needles
Part No.
Mfr. No.
Description*
AS0-1904
7758-04 RN (26/2 in./2)
AS0-4392
7758-02 RN (26/2 in./2)
AS0-4393
7758-03 RN (22s/2 in./2)
AS0-4395
7779-01 RN (22/2 in./2)
AS0-4398
7779-03 RN (22s/2 in./2)
AS0-4396
7770-02 RN (22/2 in./3)
AS0-4394
7770-01 RN (22s/2 in./3)1
AS0-4397
7780-04 RN (22/2 in./3)1
AS0-4400
7780-03 RN (22s/2 in./3)1
AS0-4402
8648-01 RN (25s/1.97 in./3)2
AS0-4401
7786-01 RN (23s/1.71 in./AS)3
The positive rear stop (in 100 L and larger sizes) prevents plunger
blowout at elevated pressures, protecting against sample loss or
operator injury. Like the Series A gas syringe, the A-2 has all the
standard Pressure-Lok features such as a PTFE plunger tip, PTFEsealed needle and ultra-smooth bore. Replacement components
are available for easy repair.
Price
*Choose larger volume syringe to reduce force on syringe filter membrane. 10 mL syringe is recommended.
For Syringe Filters, see pp. 10-12
Phenomenex
Syringes continued on next page.
369
Syringes
SGE Syringes
SGE Gastight Luer Lock Syringes
For Valco, Rheodyne, Waters, PerkinElmer,

and CTC/Leap
The syringes are fitted with a special dome-tipped removable needle to prevent needle seal damage in the valve and are of a specific
length to avoid rotor damage. A low-cost 10 L syringe, with fixed
needle and replaceable plunger, is available for all valves.
The Gastight (GT) range of gas- and liquid-tight syringes has all
the excellent features necessary to perform exacting tasks in GC,
HPLC, and other applications, particularly where inertness and
accuracy are of paramount importance. Replaceable plungers are
fitted with PTFE tips which have been machined to provide a precision fit into the glass barrel.
Syringe capacities from 1 to 100 mL also have PTFE coated plunger
stems. In addition, 1 to 100 mL syringes can be purchased with a
PTFE center male Luer to accept Luer Lock needles. Luer Lock
needles are not provided and must be ordered separately. Needles
are provided in packages (see below).
| Syringes
SGE Syringes
Part No.
Syringes for Rheodyne/Valco (2 in. Fixed Needle)
AS0-0142 002301 10F-LC
AS0-0143 003300 25F-LC
AS0-0144 004300 50F-LC
AS0-0145 005300 100F-LC
AS0-0146 006300 250F-LC
AS0-0147 007300 500F-LC
Syringes for Rheodyne/Valco (2 in. Removable Needle)
AS0-4370 002313 10R-GT-LC(SS)
AS0-4371 003312 25R-GT-LC(SS)
AS0-4373 005312 100R-GT-LC(SS)
AS0-4374 006312 250R-GT-LC(SS)
AS0-4375 007312 500R-GT-LC(SS)
AS0-4376 037250 Replacement Needles
N10-LC, #2/0.028 in. OD/2 in.
AS0-4377 038250 Replacement Needles
N25/500-LC, #2/0.028 in. OD/2 in.
Syringes for PerkinElmer (Fixed Needle)
AS0-7636 001957 SF-PE-GT-0.63
Syringes for CTC/Leap (Fixed Needle)
AS0-7638 002981 10F-C/F-S/O.63
Capacity
(L)
Unit
10
25
50
100
250
500
ea
ea
ea
ea
ea
ea
10
25
100
250
500
ea
ea
ea
ea
ea
5/pk
5/pk
ea
10
ea
Price
SGE Luer Lock Syringes
Part No.
Mfr. No.
Description
Capacity (mL)
AS0-0120
008025
1MF-LL-GT
1
AS0-0121
008760
5MDR-LL-GT
5
AS0-0122
008960
10MDR-LL-GT
10
Luer Lock and Luer Tip Needles
AS0-2016
039802
Needles 50 mm length, 23 gauge,
20 bevel tip (style #2)
AS0-2017
039803
Needles 50 mm length, 23 gauge,
side hole with dome tip (style #5)
Unit
ea
ea
ea
Price
5/pk
2/pk
For GC Syringes, see pp. 108-111
370
Phenomenex
Tubing
Tubing for HPLC
Selecting the Right Tubing and Fittings
Every chromatographer knows selecting and working with tubing
and fittings can be confusing at times. You must become knowledgeable with the plumbing of your HPLC system in order to do any
practical HPLC work. The primer below offers general guidelines as
it applies to Phenomenex products. For more detail on the subject
we recommend reading in Troubleshooting LC Systems by John
Dolan and Upchurch Scientifics guidebook to HPLC fittings.
Tubing
Most HPLC systems are plumbed with 1/16 in. OD tubing. The choice
of tubing material is based on its chemical resistivity. Stainless steel
should be avoided if the mobile phase contains high levels of strong
acids or halogenated salts. PEEK is useful with most solvents, but
should be avoided when 100 % THF, chlorinated solvents, or high
concentrations of acids are used. Titanium is virtually inert to most
chemicals and biocompatible too.
Tubing lengths and IDs should always be kept to a minimum to
minimize extra-column dead volume and band broadening. The
most common IDs used analytically are 0.007 and 0.010 in. For
Semi-prep and preparative separations, 0.020 to 0.040 in. ID tubings are typically chosen. Low pressure inlet tubing, often made of
Teflon or PEEK, is either 1/16 or 1/8 in. OD and at least 0.031 in. ID to
avoid cavitation. Inlet solvent filters of 2 to 10 m porosity are commonly used, with the same considerations above given to chemical
resistivity of the frit material.
Fittings
The tubing and fittings that make the connections from one component of the HPLC to the others must be minimized with respect
to dead volume, mixing and band broadening. All Phenomenex column hardware are female / inverted (internal type) and utilize 10-32
type fittings to attach 1/16 in. tubing of any kind (PEEK, stainless steel
or titanium). 1/4 in.-28 type fittings are also commonly used in HPLC
for other system components. A male nut and ferrule (sometimes it
is one piece, as with our convenient fingertight fittings) is used to
swage or tighten the tubing onto the fitting. You can use ferrules
interchangeably, but once the ferrule is swaged to a particular type
of fitting, it should be used with that type of fitting only.
Not only is the shape of the swaged ferrule different among different compression fittings, but the length of the tubing nib from the
ferrule to the end (the seating depth) will also differ, causing leakage
or dead volume and degraded chromatography. The exception to
this is Phenomenex, Valco, Parker, Swagelok and Upchurch fittings
which are all interchangeable at not only the initial assembly but
in subsequent assemblies too. And of course, fingertights may be
used (and repeatably reused) with any fitting of the same thread
size.
Remember to take care to seat the tubing all the way down into
the fitting, then tighten until leak-free (usually 1/4 turn) but not overtighten. Flangeless fittings (see p. 364) are generally used at the low
pressure ends of the HPLC system, for connecting inlet tubing and
filters, or for attaching fraction collectors or solvent recyclers.
Polymeric
Fingertight
Male Nut
Tubing Diameter
Inlet/Outlet Low Pressure Tubing:
1/16 in. OD x 0.030 in. ID
32 threads per inch

No. 10 or 0.190 in. diameter
Ferrule
1/8 in. OD x 0.062 in. ID
1/16 in. OD x 0.010 in. ID
tubing
High Pressure Tubing:
Male Nut
Tubing Material
Compatibility
Stainless Steel (Type 316)
AVOID high concentrations of acids or

halogenated salts
PEEK (biocompatible)
AVOID 100 % THF, chlorinated solvents, high

concentrations of acids
Titanium (biocompatible)
Compatible with nearly all chemicals
/4 in. OD Standard Analytical
Seating depth of the nib

is the critical dimension
10-32 Threaded
Male Nut and
Ferrule
(250 x 4.6 mm ID)
Female/Inverted
(internal) Column
End-fitting
Maximum Temperature Ratings (C)
Tubing Applications
Tubing ID (inch)
Column IDs (mm)
Typical Flow Rates (mL/min)
0.002
0.005
0.007
0.010
0.020
0.040
0.30 (fused silica)

1.0 (stainless steel)
2.0 - 4.6
3.2 - 7.8
10.0 - 21.2
21.2 - 100.0
0.001 - 0.02
0.02 - 0.1
0.2 - 2.0
0.5 - 5.0
2.0 - 50.0
10.0 - 200.0
Phenomenex
1/16 in. OD Tubing

(0.010 in. ID)
Tubing Compatibility
Material
PEEK
Delrin
Teflon (PFA)
Polyethylene (UHMW)
Polypropylene
KEL-F
Tefzel
Tubing
Fitting
100
N/A
80
N/A
N/A
N/A
80
150
60
80
50
40
80
80
371
Tubing
Capillary PEEK
HPLC Tubing
n
e
w
PEEKsil Tubing
for UHPLC / HPLC
Chemically inert and biocompatible
Minimizes extra-column effects and band broadening
Precision-cut, ultra-square tube ends
Easily bendable and cuttable
Exceptionally smooth inner surfaces
PEEK HPLC tubing is an excellent alternative to stainless steel

tubing for most HPLC applications. PEEK (polyetheretherketone) is
chemically inert to virtually all HPLC solvents (only 100 % methylene
chloride, THF, concentrated nitric acid or concentrated sulfuric acid
will affect PEEK), and is 100 % biocompatible. PEEK tubing can be
used with stainless steel nuts and ferrules or polymeric fittings such
as the Phenomenex fingertight Sure-Lok fittings (see p. 363).
PEEKsil is polymer-sheathed fused silica tubing. The PEEK portion

is mechanically strong and has ideal sealing characteristics when
used with conventional metal or PEEK ferrule systems. Capable
of withstanding high pressures up to 10,000 psi (689 bar), the
exceptionally smooth inner surfaces are free of the imperfections
common in steel tubing, which lessens the possibility of path
blockages, ultimately providing lower band broadening. The
precision-cut, ultra-square and smooth tube ends enable optimal
low volume connections to be made, which will improve overall
chromatographic performance. For higher efficiencies and improved
resolution, PEEKsil tubing is recommended to help optimize your
UHPLC system. For critical UHPLC connections a convenient
fittings and tubing kit Part No.: AQ0-8892 is available (see p. 362).
This PEEK HPLC tubing is pressure tested to 7,000 psi and rated at
5,000 psi for continuous use (for standard 0.010 in. ID tubing). PEEK
withstands extremely high temperatures and is rated for continuous
use up to 100 C.
PEEK tubing is color coded for easy identification. All colors are
permanent and there is no leaching.
A PEEK TUBING KIT is available that contains all the PEEK tubing
normally required to replumb a complete HPLC system, including
5 ft. each of: 0.005 in. x 1/16 in., 0.007 in. x 1/16 in., 0.010 in. x 1/16 in.,
0.080 in. x 1/8 in. tubing, and a Polymer tubing cutter.
Polymer
Outer Sheath
OD ID
PEEKsil tubing showing the
PEEKsil tubing showing the

precision
ground
ground
and square
cut endand square
enabling
a zero
dad volume
cut end
enabling
a zero dead
connection.
Fused Silica Lining

Polyimide
volume connection.
PEEKsil Tubing for UHPLC/HPLC
Part No.
Description
Related Accessory Items
AQ0-8503 Sure-Lok High Pressure PEEK 1-Pc Nut,
10-32, for 1/16 in. Tubing
Price
10/
pk
ea
PEEKsil is compatible with most organic solvents. Effective pH

range from 0 to 10.
| Tubing
372
Unit
5/pk
5/pk
Standard PEEK
Straight PEEK Tubing
Length
OD
ID
Part No.
(feet)
(inch)
(inch)
AT0-1107
5
1/16
0.010
1/16
AT0-1260
5
0.007
1/16
AT0-1259
5
0.005
Part No.
Description
PEEK Tubing Kit
AT0-1964 PEEK Tubing Kit, includes one each of:
AT0-1259 (5 ft. x 1/16 in. x 0.005 in.)
AT0-1260 (5 ft. x 1/16 in. x 0.007 in.)
AT0-1107 (5 ft. x 1/16 in. x 0.010 in.)
AT0-1265 (5 ft. x 1/8 in. x 0.080 in.)
AT0-1110 (Polymer Tubing Cutter)
Part No.
Description
Polymer Tubing Cutter
AT0-1110 Polymer Tubing Cutter
For more information on Part Nos. AQ0-8503

and AQ0-8530, see p. 361
See also our UHPLC connections kit on p. 362
Capillary Stainless Steel Tubing

Ready to use
Color
blue
yellow
red
Unit
ea
ea
ea
Unit
Price
Precut and polished ends

Price
ea
Unit
ea
Passivated and solvent rinsed
Price
Capillary Stainless Steel Tubing
Part No.
0.005 in. ID x 0.062 in. (1/16 in.) OD
AT0-2996
AT0-2997
AT0-2998
0.010 in. ID x 0.062 in. (1/16 in.) OD
AT0-0456
AT0-0457
AT0-0458
AT0-0460
AT0-0461
0.020 in. ID x 0.062 in. (1/16 in.) OD
AT0-0465
AT0-0466
AT0-0469
Length
Unit
5 cm
10 cm
20 cm
5/pk
5/pk
5/pk
5 cm
10 cm
20 cm
50 cm
1m
5/pk
5/pk
5/pk
2/pk
2/pk
10 cm
20 cm
1m
5/pk
5/pk
2/pk
Price
Phenomenex
Tubing
Flex-Coil Capillary PEEK Tubing
Tubing Cutters
Flex-Coil tubing is made from biocompatible, chemically inert PEEK

(polyetheretherketone) material. The flexible construction of this
tubing allows it to expand and contract, making installation of tubing and system configuration quick and easy. The Flex-Coil design
also helps to prevent crimping and deforming of your tubing.
Terry Tools are compact tubing cutters for either 1/16 or 1/8 in. OD
glass-lined tubing or stainless steel tubing. The specially-hardened
cutting wheels make clean, right-angle cuts with minimal burring or
chipping of the tubing. Zero dead-volume connections, essential
in most GC and MS and all HPLC plumbing applications, can be
easily achieved. No additional reaming or deburring is required and
nuts and ferrules slip on easily. Extra cutting wheels are available.
Order fittings separately.

Phenomenex recommends
Sure-Lok fingertight
fittings, see p. 363
Flex-Coil PEEK
Flexible Coil PEEK Tubing
Length
OD
Part No.
(cm)
(inch)
1/16
AT0-2003
10
1/16
AT0-2004
20
1/16
AT0-2005
40
1/16
AT0-2006
10
1/16
AT0-2007
20
1/16
AT0-2008
40
Terry Tool
Stainless Steel Tubing Cutter
ID
(inch)
0.010
0.010
0.010
0.005
0.005
0.005
Volume
(L)
5
10
20
1
3
5
Color
blue
blue
blue
red
red
red
Unit
ea
ea
ea
ea
ea
ea
Price
The Polymer Tubing Cutter
makes clean, square cuts
on both 1/16 and 1/8 in. OD
polymeric tubing, especially
PEEK tubing
Teflon (PTFE) Tubing

Pressure rated to 500 psi (35 kg/cm2)
ea
Wall Thickness
(inch)
0.026
0.026
0.015
0.015
0.030
0.030
0.060
0.060
Length
(feet)
5
10
5
10
5
10
5
10
Price
Wrenches/Spanners
Phenomenex offers three of the most popular sizes of wrenches
(spanners) used in UHPLC/HPLC.
Real Rheodyne Fitting Wrench
Part No.
AV0-4219
6810
The Real Rheodyne Fitting Wrench
*For additional information, see p. 378
Unit
ea
Wrenches/Spanner
Part No.
Description
AQ0-8903
Wrench, Open End, 1/4 x 5/16 in.
AQ0-8959
AQ0-8904
Price
Unit
ea
ea
ea
ID
(inch)
0.010
0.010
1/32 (0.031)
1/32 (0.031)
1/16 (0.062)
1/16 (0.062)
1/8 (0.125)
1/8 (0.125)
Rheodyne Fitting Wrench
Phenomenex
Price
Teflon Tubing
OD
Part No.
(inch)
1/16
AT0-2951
1/16
AT0-2952
1/16
AT0-2953
1/16
AT0-2954
1/8
AT0-2955
1/8
AT0-2956
1/4
AT0-8609
1/4
AT0-8610
Unit
ea
ea
3/pk
tubing
Tubing Cutters
Part No.
Description
AQ0-1305
1/16 in. Terry-Tool tubing cutter
AQ0-1306
1/8 in. Terry-Tool tubing cutter
AQ0-1307
Replacement cutting wheels for both
AQ0-1305 and AQ0-1306
AT0-1110
Resistant to virtually all corrosive chemicals

and organic solvents
Price
SecurityGuard ULTRA installation onto Kinetex columns and

later cartridge replacement requires 3 wrenches, which must be
purchased separately: one 3/8 in. wrench (AQ0-8959; fits Kinetex
column end-fitting), and two 5/16 in. wrenches (AQ0-8903); fits
ULTRA cartridge and holder).
See SecurityGuard ULTRA p. 239
373
Tubing
Empty HPLC Column
Assemblies
Frits
316 stainless steel
1/16 and 1/32 inch thicknesses
Stainless Steel
0.5 and 2.0 m pore sizes
Thoroughly washed, ultrasonically cleaned

and passivated
Highly polished mirror-finish tubing
Female/inverted Type 316 stainless steel end-fittings,
4 stainless steel frits and 2 column plugs included
Wide selection of column lengths and diameters
To ensure proper frit selection, match the frit pore size with the
packing material particle size (Table 1). Problems may occur if
a large pore frit is used with a small particle size material. This
combination can lead to the packing material escaping or
clogging the frit, resulting in shortened column life or a damaged
flow cell.
Table 2 will assist you in choosing the proper size (diameter,
thickness, and porosity) frit in relation to the material particle size
and column internal diameter.
| Tubing
Table 1. Frit Pore Size Selection Guide
Stainless Steel Empty HPLC Column Assemblies
Part No.
ID (mm)
OD (inches)
1/8
AQ0-3019
1.0
1/8
AQ0-3020
1.0
1/8
AQ0-3021
1.0
AQ0-0182
2.0
AQ0-0183
2.0
AQ0-0185
4.0
AQ0-7262
4.6
AQ0-0186
4.6
AQ0-0187
4.6
AQ0-0188
4.6
AQ0-0189
4.6
3/8
AQ0-0195
7.8
AQ0-0197
10.0
AQ0-7849
21.2
1
AQ0-7499
21.2
1
AQ0-0200
21.2
1
Length (mm)
100
150
250
150
250
250
30
50
100
150
250
300
250
100
150
250
When particle size of your material is:
Frit pore size should be:
2-4 m
0.5 m
5-20 m
2 m
Price
Table 2. Frit Size Selection Guide

Material
Particle
Size (m)
2-4
5 - 20
2-4
5 - 20
5 - 20
5 - 20
5 - 20
Column
Internal Diameter
(mm)
Frit
Diameter
(inches)
Frit Thickness
(inches)
Frit
Pore Size
(m)
1.0
1.0
2.0-5.0
2.0-5.0
6.0-8.0
9.0-10.0
11.0-23.0
1/8
1/8
1/4
1/4
3/8
1/2
1
1/32
1/32
1/32
1/32
1/32
1/32
1/16
0.5
2.0
0.5
2.0
2.0
2.0
2.0
Column End-Fittings
HPLC Column End-Fittings**
Part No.
Size (Column-Capillary)
1
AQ0-1874
/8 in. - 1/16 in. (for 1.0 mm ID)
Unit
ea
AQ0-1875
/4 in. - 1/16 in. (for 2.1 to 4.6 mm ID)
ea
AQ0-1876
/8 in. - 1/16 in. (for 7.8 mm ID)
ea
AQ0-1877
/2 in. - 1/16 in. (for 10.0 mm ID)
ea
AQ0-1878
1 in. - 1/16 in. (for 21.2 mm ID)
ea
Price
Frits
Diameter Thickness Pore Size
Part No.
(inches)
(inches)
(m)
1/8
1/32
AQ0-0537
2.0
1/4
1/32
AQ0-0538
0.5
1/4
1/32
AQ0-0539
2.0
3/8
1/32
AQ0-0541
2.0
1/2
1/32
AQ0-0543
2.0
1/16
AQ0-8166*
1
2.0
* Only for use with Column Assembly Part No. AQ0-0200.
Unit
10/pk
10/pk
10/pk
10/pk
10/pk
2/pk
Price
All Phenomenex Valco-compatible end-fittings

listed are female/inverted (internal threaded) and
compatible with 10-32 threaded male nuts.
For Fittings, see pp. 360-364
**Includes male and female end fittings with ferrule and column
sealing plug. Purchase frits separately.
374
Phenomenex
Valves
Sample Injector
Rheodyne 7725
Sample loading by syringe through built-in needle port
Continuous flow during switching (no interrupt)
A front-end pressure screw for easy seal adjustment
sample loop
Wide port angles for improved access to fittings

Pressure rated to 7000 psi (490 kg/cm2)
to column
from pump
needle port
5 L to 5 mL removable sample loops
stator
stator face
rotor seal
needle
2 L internal sample loop accessory

Internal position switch (7725i version only)
The Model 7725 and 7725i are Rheodynes most advanced
sample injectors for analytical HPLC. They can inject from 2 L to
5 mL with high accuracy and precision. These versatile injectors
can use either of two methods for loading the sample loop.
The partial-filling method uses the syringe to measure the sample
volume. lt lets you vary volume easily without having to change
the loop, and it does not waste any sample. Any amount up to
half the loop volume can be loaded. The amount injected into the
column is the exact volume dispensed from the syringe. Precision is about 1 %.
The complete-filling method uses the loop to measure the volume. lt lets you load the loop without having to read syringe calibrations carefully. Excess sample is required to completely flush
mobile phase from the loop. The amount injected is the volume of
the loop. Precision is typically 0.1 %. Ten interchangeable loops
ranging from 5 L to 5 mL are available.
Description
Sample Injector1
Sample Injector, with Position
Sensing Switch1
Price
Sample Loops2
AV0-2349
7755-020
5 L Sample Loop
AV0-2350
7755-021
10 L Sample Loop
AV0-2351
7755-022
20 L Sample Loop
AV0-2352
7755-023
50 L Sample Loop
AV0-2353
7755-024
100 L Sample Loop
AV0-2354
7755-025
200 L Sample Loop
AV0-2355
7755-026
500 L Sample Loop
AV0-2356
7755-027
1 mL Sample Loop
AV0-2357
7755-028
2 mL Sample Loop
AV0-2358
7755-029
5 mL Sample Loop
Spare Replacement Parts for Model 7725 Injector
AV0-3500
7725-999
Complete RheBuild Kit for
valves 7725, 7725i, 7726
(see p.378 for description)
AV0-0169
7125-047
Vespel Rotor Seal
AV0-2416
7125-079
Tefzel Rotor Seal
AV0-2362
7725-026
Stator Face Assembly
AV0-0171
7125-054
Needle Port Cleaner
AV0-0180
7215
#22-Gauge Needle
AV0-2365
6000-263
Nut 10pk
AV0-2366
6000-264
Long Nut 10pk
AV0-2367
6000-265
Extra Long Nut 10pk
AV0-2368
6000-110
Ferrule 5pk
The 7725 and 7725i have a 20 L stainless steel loop installed.

They are supplied with instructions, fittings for all ports, needle port cleaner, two vent tubes, two hex wrenches, mounting
screws, and a #22-gauge needle with Luer hub. Maximum operating temperature is 80 C. The 7725i has an internal position
sensing switch with six-foot lead wires. Installation of a 2 L internal loop requires partial disassembly of the injector.
7725 Sample Injectors
Part No.
Mfr. No.
AV0-2346
7725
AV0-2347
7725i
RIGHT: In INJECT, sample is displaced

from the loop to the column by mobile
phase from the pump. The needle port is
automatically isolated from pressure.
The patented needle port connects the tip of the needle directly
to the sample loop, so there is no connecting passage that can
trap sample. This assures syringe accuracy, results in zero sample
waste, and makes it unnecessary to flush after every injection to
avoid cross contamination (except in trace analysis).
LEFT: In LOAD, mobile phase flows

from the pump to the column via
a rotor seal groove (not shown).
Meanwhile sample is dispensed into
the loop, displacing atmosphericpressure mobile phase out a vent line
(not shown).
valves
Flow switching occurs at a flat interface between a polymeric rotor seal and a ceramic stator face. This combination provides long
seal life. In a clean system the 7725 typically can make more than
30,000 injections before requiring replacement of the rotor seal.
The diagram illustrates the simple operation: Insert a syringe into
the needle port, dispense the desired amount of sample into the
loop, then turn the handle to the INJECT position. The grooves
in the rotor seal now redirect the flow. Sample passes out the
beginning of the loop, without flowing through its entire length.
The loop is completely flushed by keeping the handle in the INJECT position during the analysis. After removing the syringe and
returning to LOAD, you are ready for the next injection.
syringe
Syringes for Rheodyne sample injectors are listed on pp. 368, 370.
Designed for the Model 7725 Injectors new, wide-angle ports.
These stainless steel loops are not interchangeable with loops for
the Model 7125 Injector.
Phenomenex
375
Valves
Sample Injector
Sample Injectors
Rheodyne Injector Model 9725

Totally Metal-Free (PEEK)
Rheodyne 3725 and 3725-038 Preparative

For preparative HPLC columns, 1 to 10 cm ID
Inert flow passages of Tefzel, PEEK, and

alumina-ceramic (pH range 0 to 14)
Not affected by buffers, acids, bases or halide salts
For low-pressure soft gel chromatography and HPLC
Uses Rheodyne RheFlex fittings for high-pressure
PEEK tubing (See p. 377 for RheFlex fittings information)
Versatile... All techniques and applications!
Complete fill 5 L to 5 mL sample loops using excess
sample
Partial-fill 0.1 L to 5 mL with zero sample waste
Valve will operate to 5000 psi
Loops will operate to 5000 psi depending on ID and
solvent
Preparative Sample Injectors
Part No.
AV0-2053
3725
PEEK Preparative Sample Injector
AV0-2054
3725i
PEEK Preparative Sample Injector,
with Position Sensing Switch
AV0-2055 3725-038 Stainless Steel Preparative Sample Injector
AV0-2056 3725i-038 Stainless Steel Preparative Sample Injector,
with Position Sensing Switch
AV0-3432 3725-999 Complete RheBuild Kit
for valves 3725, 2715i, 3725-038,
3725i-038 (see p. 378 for description)
Use with 1 to 2 mm ID micro, 3 to 6 mm analytical or

milligram-scale prep columns
Price
| Valves
Switching Valve/Injector
PEEK Sample Injectors
Part No.
AV0-1074
9725
PEEK Sample Injector
AV0-4642
9725i
PEEK Sample Injector, with Position Sensing Switch
AV0-1086 9125-076 Suction Needle Adapter
AV0-2375 9125-123 Needle Port Tube Assembly
AV0-3433 9725-999 Complete RheBuild Kit for valves 9725, 9725i
(see p. 378 for description)
Rheodyne Valve Model 7000

Price
Enables sample clean-up and trace sample enrichment

Enables column programming and backflushing
Enables dual-column selection
Field-changeable switching patterns
Although PEEK material is highly resistant to most chemicals,

PEEK is not recommended for applications requiring high
concentrations of THF (Tetrahydrofuran), methylene chloride,
nitric acid or sulfuric acid.
Sample Injector
Rheodyne 8125 Low-Dispersion
Suitable for both microbore and analytical HPLC
Accurately inject as little as 0.1 L of sample
Improve peak resolution
Low-Dispersion Sample Injector
Part No.
AV0-0181
8125
Low-Dispersion Sample Injector
AV0-3431 8125-999 Complete RheBuild Kit for valve 8125
376
Permits column switching and selection in various

configurations
Price
Switching Valves
Part No.
AV0-2376
7000
Switching Valve/Injector
AV0-2378
7010
Sample Injection Valve
AV0-3430 7010-999 Complete RheBuild Kit for valves 7010, 7000
AV0-1073
7012
Loop Filler Port
AV0-1092
9010
PEEK Switching Valve/Injector
AV0-2381
9013
PEEK Needle Port
Price
Phenomenex
Valves
RheFlex Fittings
Sample Injector Loops
The nut and ferrule are made of PEEK, an exceptionally strong and
inert polymer. The fitting holds with finger tightening on a variety of
plastic and metal tubing. The nut and ferrule can both be reused
many times. The fittings are sold as a nut and ferrule set. Multicolored knobs are available to aid identification of connections.
Stainless steel external loops are supplied with unattached fittings

so the tube can be completely bottomed in the injector port before
the ferrule is swaged on. RheFlex PEEK loops do not require this
precaution, because the ferrule can slide and reposition itself along
the tube when the fitting is reinserted into a port.
Knob
Nut
Ferrule Assembly
RheFlex Fittings
Part No.
AV0-2383 6000-054 RheFlex Std. Fitting Set (5 nuts and 5 ferrules)
AV0-2384 6000-055 RheFlex Short Fitting Set (5 nuts and 5 ferrules)
AV0-2385 6000-066 RheFlex 33 mm Fitting Set (1 nut and 1 ferrule)
AV0-2386 6000-051 RheFlex Ferrules (5 ferrules)
AV0-5810 6000-283 ChromTRAC Fittings Identification Kit,
multi-colored mix
Unit
ea
ea
ea
ea
20/pk
Price
Unit
Price
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
1. Loops for the 7725 Valve are listed with the valve on p. 375.
2. Loops not listed for other valves are available. Call your
Phenomenex Technical Consultant.
ea
ea
ea
ea
ea
ea
ea
ea
ea
ea
Valves
Sample Injector Loops
Part No.
Mfr. No.
Description
Stainless Steel Loops for 7125 and 7010 Valves
AV0-2390
7020
5 L, 0.007 in. ID
AV0-2391
7021
10 L, 0.012 in. ID
AV0-2392
7022
20 L, 0.020 in. ID
AV0-2393
7023
50 L, 0.020 in. ID
AV0-2394
7024
100 L, 0.020 in. ID
AV0-2395
7025
200 L, 0.030 in. ID
AV0-2396
7026
500 L, 0.030 in. ID
AV0-2397
7027
1 mL, 0.030 in. ID
AV0-2398
7028
2 mL, 0.040 in. ID
AV0-2399
7029
5 mL, 0.040 in. ID
Loops for 8125 Low Dispersion Injector ( Stainless Steel)
AV0-2937
8020
5 L, 0.008 in. ID
AV0-2938
8021
10 L, 0.008 in. ID
AV0-2939
8022
20 L, 0.010 in. ID
AV0-2940
8023
50 L, 0.012 in. ID
PEEK (for all valves)
AV0-1076
9055-020
5 L, 0.007 in. ID
AV0-1077
9055-021
10 L, 0.010 in. ID
AV0-1078
9055-022
20 L, 0.010 in. ID
AV0-1079
9055-023
50 L, 0.020 in. ID
AV0-1080
9055-024
100 L, 0.020 in. ID
AV0-1081
9055-025
200 L, 0.020 in. ID
AV0-1082
9055-026
500 L, 0.030 in. ID
AV0-1083
9055-027
1 mL, 0.030 in. ID
AV0-1084
9055-028
2 mL, 0.030 in. ID
AV0-1085
9055-029
5 mL, 0.030 in. ID
Rheodyne MX Series II automated fluidic valves work with

your instrument to provide productivity enchancing solutions
for complex fluid switching and sample injection applications.
Ask your Phenomenex Technical Consultant for more information.
Phenomenex
377
Valves
RheBuild Kits
Valve Stators
Each kit contains all the parts necessary to refurbish the corresponding valve. For front-loading injection
valves, the kit includes: rotor seal,
stator face assembly, isolation seal,
needle guide, needle port cleaner,
2 hex keys and repair instructions.
Type 70's Switching Valves and
Model 7010 Injector Valve do not
include Stator Face Assemblies.
Refer to the specific valve on the
previous pages for ordering information.
Valve Stators
Part No.
AV0-0172
7125-067 Stator Face Assembly for Valve Model 7125
AV0-4719
7725-010 Stator for Valve Models 7725, 7725i
AV0-0175
7010-040 Stator for Valve Models 7000, 7010, 7125,
7030, 7040
AV0-2422
8125-098 Stator for Valve Model 8125
AV0-2423
9125-043 Peek Stator & Support Ring for 9010/9125
Rheodyne Fitting Wrench

Slotted Wrench for Rheodyne Valves
Fits around tubing to tighten any 1/4 or 5/16 in. nut
Unit
ea
ea
ea
Price
ea
ea
Rotor Seals
Access hard-to-reach areas

Saves time and effort
| valves
Real Rheodyne Fitting Wrench
Part No.
AV0-4219
6810
The Real Rheodyne Fitting Wrench
Unit
ea
Price
Syringe and Injector

Accessories
The #22-gauge needle (Mfr. No.
7215) has a Kel-F luer hub that
fits any luer tip syringe.
Model 7160 and 7160-010
mounting bracket (shown below)
accommodate all Rheodyne high
pressure injectors and valves.
Unit
Price
ea
ea
ea
ea
ea
ea
ea
ea
All other Rheodyne valves and accessories not listed

are available.
Syringe and Injector Accessories
Part No.
AV0-0180
7215
#22-Gauge Needle with CTFE Luer Hub
AV0-0170
7125-008 Needle Guide
AV0-0171
7125-054 Needle Port Cleaner
AV0-2425
7160-010 Valve Angle Bracket
AV0-2426
7160
Mounting Panel
Unit
ea
ea
ea
ea
ea
Price
Syringes for Rheodyne sample injectors are listed on pp. 368, 370.
378
Rotor Seals
Part No.
Mfr. No.
Description
Vespel (pH Range 0 to 10)
AV0-2412
7010-039 Rotor Seal for Valve
Models 7000, 7010, 7040
AV0-0169
Models 7125, 7725, 7725i
AV0-2414
8125-038 Rotor Seal for Valve Model 8125
Tefzel (pH Range 0 to 14)
AV0-2415
Models 7000, 7010, 7040
AV0-2416
Models 7125, 7725, 7725i
AV0-2417
Model 8125
AV0-2418
Model 9010
AV0-2419
Models 9725 and 9125
For Column Selectors, see p. 358

For Stainless Steel and PEEK Capillary Tubing
used with Rheodyne valves, see p. 372
Phenomenex
Appendices
Abbreviations............................................................................................... 380
Chromatogram Index...........................................................................390-400
Chromatographic Parameters..................................................................... 384
GC Column Installation Instructions.....................................................381-382
GC Column MS Certification - Zebron........................................................ 382
HPLC Calculations...................................................................................... 383
HPLC Column Protection............................................................................ 386
HPLC Material Sorbent Characteristics...............................................388-389
Product Index.......................................................................................401-405
Reversed Phase Method Development................................................384-385
Probes for Column Characterization...................................................... 384
Solvent Miscibility Table.............................................................................. 387
Terms and Conditions of Sale..................................................................... 407
Trademarks.................................................................................................. 406
APPENDICES
379 - 408
Abbreviations
Appendices
ABBREVIATIONS
ACN
AGP
alpha
amu
a
aq. sol.
AUFS
BET
380
BHT
BSA
BuOAc
C
C1
C18
C2
C3
C4
C5
C6
C8
CC
CCl4
CH3COOH
CHCl3
CI
CH2Cl2
CLP
cm
CM
CMC
CN
COOH
CSP
CTA
CTAB
CuAc
CuSO4
CV
D
Da
Dabsyl
Dansyl
DC
DCM
DEAE
DEAM
df
dia
DMAC
DMF
DMSO
DNPH
dp
ECD
ECDV
EDTA
ELSD
em
EPA
ESI
ETFE
EtOAc
EtOH
ex
F
FID
FLR
FMOC
FPLC
FTIR
g
GC
GFC
GLP
GMP
GnHCl
GPC
H
Angstrom
acetonitrile (methyl cyanide)
alpha-1-acid glycoprotein
alpha (separation factor)
atomic mass unit
alpha (separation factor)
aqueous solution
absorption units full scale
Brunner, Emmett and Teller method of surface
analysis
butylhydroxytoluene
bovine serum albumin
butyl acetate
Celsius
methyl silane phase (trimethyl silyl)
octadecyl silane phase
ethyl silane phase
propyl silane phase
butyl silane phase
pentyl silane phase
hexyl silane phase
octyl silane phase
chiral chromatography
carbon tetrachloride
acetic acid
chloroform
chemical ionization
dichloromethane (methylene chloride)
Contract Lab Program
centimeter
carboxymethyl
critical micelle concentration
cyano
carboxylic acid
chiral stationary phase
cellulose triacetate
cetyltrimethylammonium bromide
copper acetate
copper sulfate
coefficient of variation
depth
Dalton
4-N,N-dimethylaminoazobenzene-4-sulfonyl chloride
5-N,N-dimethylaminonaphthylene-1-sulfonyl chloride
direct current
dichloromethane (methylene chloride)
diethylaminoethyl
diethylaminomethyl
film thickness dimension (GC)
diameter
dimethylacetamide
dimethylformamide
dimethylsulfoxide
dinitrophenylhydrazine
degree of polymerization
electrochemical detection
extra column dead-volume
ethylenediamine tetraacetic acid
evaporative light scattering detector
emission (wavelength)
Environmental Protection Agency
electrospray ionization
Tefzel, ethylene tetrafluoroethylene copolymer
ethyl acetate
ethanol
excitation (wavelength)
Fahrenheit
Flame Ionization Detector
fluorescence
9-fluorenylmethylchlorformate
fast protein liquid chromatography
Fourier-transform infrared
gram
gas chromatography
gel filtration chromatography
good laboratory practice
good manufacturing practice
guanidine hydrochloride
gel permeation chromatography
height
H2SO4
H3PO4
HAC
HCl
HETP
hex
HF
HFIP
HIC
HILIC
HPCE
HPLC
Hz
IC
ID
IEC
IEX
in.
IPA
IPC
IR
ISO
ISRP
IUPAC
I/O
k
kDa
kg
kg/cm2
KH2PO4
L
lbs
LC
LCD
LC/MS
LEC
LED
g
L
m
M
mole/m2
m2/g
MB
MC
MDEA
MECC
MeCN
MeOH
meq/g
mg/mL
min
mL/g
mL/min
mm
mM
MOS
Mp
MS
MS-DOS
MSDS
msec
MTBE
mV
MW
MWD
MW/Mn
N
Na2PO4
NaCO3
NaHCO3
NaN3
NaOAc
NF
NH2
NH4Ac
NIOSH
NIST
nm
NMP
sulfuric acid
phosphoric acid
hydroxyapatite chromatography
hydrochloric acid
height equivalent to a theoretical plate
hexagonal (6-sided) nut
hydrofluoric acid
hexafluoroisopropanol
hydrophobic interaction chromatography
hydrophilic interaction liquid chromatography
high-performance capillary electrophoresis
high performance liquid chromatography
Hertz
ion chromatography
internal diameter
ion-exchange chromatography
ion-exclusion chromatography
inch
isopropanol (isopropyl alcohol)
ion pair chromatography
infrared
International Standards Organization
internal surface reversed phase
International Union of Pure and Applied Chemistry
input/output
capacity factor
kilo Daltons
kilogram
kilogram per centimeter squared
potassium dihydrogen phosphate
length
pounds
liquid chromatography
liquid crystal display
liquid chromatography/mass spectroscopy
ligand-exchange chromatography
light-emitting diode
microgram
microliter
micrometer
micromole
micromoles per meter squared
meters squared per gram
megabyte
methylene chloride (dichloromethane)
methyl-diethylamine
micellar electrokinetic capillary chromatography
methyl cyanide (acetonitrile)
methanol
milliequivalent per gram
milligram per milliliter
minute
milliliter per gram
milliliter per minute
millimeter
millimolar
monooctyl silane
peak molecular weight
mass spectrometry
Microsoft Disk Operating System
material data safety sheet
milli-seconds
methyl tert-butyl ether
milli-volt
molecular weight
molecular weight distribution
molecular weight per molecular number
efficiency
sodium phosphate
sodium carbonate
sodium bicarbonate
sodium azide
sodium acetate
National Formulary
amino
sodium acetate
National Institute of Occupational Safety and Health
National Inst. of Standards & Technology
nanometer
N-methyl pyrrolidone
NO2
NP
o-CP
OD
ODS
OH
PAC
PAH
PCTFE
PEEK
PEG
PEI
PFA
PFP
PFPA
pH
PHM
PITC
pKa
PM
PMMA
PMP
PO43ppb
ppm
PRP
PSF
psi
PTC
PTFE
PTH
PVA
PVC
PVDF
P&A
p/m
QA
QC
RAM
RI
ROM
RP
Rs
RS232
RSD
SAS
SAX
SCX
SDS
SEC
SFC
SFE
Si
sol.
SPE
SS
ST
TEA
TEAA
temp.
TFA
THF
TLC
TMS
USP
UV
V
VA
VAC
v/v
W
w
WAX
WCX
w/v
XLPE
ZDV
nitro
normal phase
o-chlorophenol
outer diameter
octadecylsilane
hydroxyl, diol or glycerol phase
polar amino cyano
polyaromatic hydrocarbon
Kel-F
polyetherether ketone
polyethylene glycol
polyethyleneimine
Teflon, (perfluoroalkoxy monomer copolymerized)
pentafluorophenyl
pentafluoropropionic acid
parts hydrogen (measure of acidity)
polyhydroxymethacrylate
phenylisothiocyanate (Edmans reagent)
dissociation constant of an acid
permethylated
polymethyl methylacrylate (acrylic)
polymethylpentene
phosphate ion
parts per billion
parts per million
polymeric reversed phase
polysulfone
pounds per square inch
phenylthiocarbamyl
Teflon [poly(tetrafluoroethylene)]
phenylthiohydantoin
polyvinyl alcohol
polyvinyl chloride
Kynar, polyvinylidene difluoride
partition and adsorption chromatography
plates per meter (N)
quality assurance
quality control
random access memory
refractive index
read only memory
reversed phase
resolution
registered standard for I/O serial interface
relative standard deviation
short alkyl silyl (C1)
strong anion-exchange
strong cation-exchange
sodium dodecyl sulfate
size exclusion chromatography
supercritical fluid chromatography
supercritical fluid extraction
silica
solution
solid phase extraction
stainless steel
standard taper
triethylamine
tetraethyl ammonium acetate
temperature
trifluoroacetic acid
tetrahydrofuran
thin-layer chromatography
trimethyl chlorosilane
United States Pharmacopoeia
ultraviolet
Volt
vanillic acid
volts alternating current
volume per volume
width
watts
weak anion-exchange
weak cation-exchange
weight per volume
cross-linked high-density polyethylene
zero dead-volume
Phenomenex
GC Column Installation Instructions

The following is a brief reminder of the general precautions required
in handling and installing any organic-coated fused silica capillary
column. Consult your GC manual for more details.
Fused silica capillary columns become brittle if the polyimide coating applied during manufacture is damaged. Avoid temperatures
above 370 C, and excessive bending, twisting, and abrasion of
columns, which will damage this protective coating. Remember,
even if the column does not break immediately, when the protective
coating is damaged the column may break spontaneously later.
Warning: It is advisable to wear safety glasses.

Warning: Cyano columns are susceptible to oxidation and
hydrolysis. Care must be taken to avoid leaks, water,
strong acids and high temperatures.
All foreign material including debris from the septa or ferrules must
be kept out of the column.
The stationary phase, which coats the inside of the column, must
also be protected. The ends of the column will be sealed or protected by a septum when you receive the column. Once the ends
are open in preparation for installation, the column should be installed in a chromatograph as soon as practical and a flow of dry,
oxygen-free carrier gas maintained until the column is removed and
resealed.
Figure 1: Proper and Improper Cut Capillary End
Installing the Column
Figure 2: Cutting Fused Silica Tubing
GC COLUMN INSTALLATION INSTRUCTIONS

|
Figure 3: Column Hanging Diagrams
Appendices
A. Instrument and Capillary Column Preparation

1. Turn off all heated zones and allow them to cool.
2. Make sure you have carrier gas of sufficient purity; replace
carrier gas purifiers, if appropriate.
3. Clean and deactivate injector and detector sleeves as
necessary.
4. Replace seals and septum, and deactivate liner if necessary.
5. Inspect the column for damage.
6. Cut a centimeter or two off an end of the column. Use a
sapphire scribe or a ceramic scoring-wafer to score the
tubing before breaking it.
7. While pointing the end of the column down, install a nut
and ferrule on it. Make sure the ferrule is the right size and
pointed in the correct direction.
8. Cut an additional centimeter or two from the end of the
column to remove ferrule fragments. Check the end. A
20-power magnifying glass is recommended. If the break is
not clean and the end square, cut the column again.
9. Mount the column in the GC oven without damaging the
column coating. It should not have sharp bends or touch the
walls of the oven.
10. Insert the column into the injector exactly the correct distance
specified in the instrument manual. Use correction fluid to
mark the exact insertion distance.
11. Tighten the ferrule nut until the column resists movement.
One-quarter turn past finger tight is about right. Do not
connect the column to the detector at this time.
12. Adjust the head pressure to obtain the flow rate listed on the
test chromatogram.
13. Check the inlet connections for leaks.
14. Confirm gas flow through the column by observing bubbles
when the column outlet end is immersed in a vial of nontoxic
solvent such as acetone.
15. Set gas flow rates for the detector including the make
up rate.
Warning: Avoid sharp bends when installing columns.
Continued on next page
Phenomenex
381
Appendices
382
B. Conditioning and Testing the Capillary Column

16. Purge the column with carrier gas for approximately 15 minutes. Further conditioning may be desirable.
17. Insert the outlet end of the column into the detector exactly
the distance prescribed in the instrument manual, repeating steps 7 through 9. Use correction fluid to mark the exact
insertion distance.
18. Set gas-flow rates to instrument specifications. Warning!
Some detectors may be damaged by heating without proper
gas flow.
19. Check the system for leaks. It is preferable to use a thermalconductivity-type leak detector. Do not use soaps or liquidbased leak detectors with capillary columns. Never heat the
column without checking thoroughly for leaks first.
20. Set injector and detector temperatures. Turn the detector on
when steady state temperatures are achieved.
21. Increase the oven temperature to the maximum continuous operating temperature for the column. Warning! Do not
exceed the maximum operating temperature of the column.
Maintain that temperature until a flat baseline is observed. If
this takes more than half an hour, it could indicate a problem.
22. Inject a detectable unretained sample such as methane to
determine dead volume time and linear gas velocity. Adjust
gas pressure to obtain proper values for your analytical
method.
23. Set oven to starting temperature. Inject another sample of a
detectable unretained substance. Reset the carrier gas velocity to desired value.
24. Check the performance of the GC and the column by injecting a known sample or performance test mix. If all peaks tail,
it could indicate loose fittings, improper column installation,
or broken liner. See the Section on Troubleshooting Installation Problems.
25. Calibrate the instrument.
26. Inject a sample, ensuring that the vaporized sample volume
does not exceed the inlet sleeves buffer volume
27. For short-term standby operation of the GC instrument
continue carrier gas flow at 100-200 C. Long term standby
conditions require that the column be removed from the instrument, flame-sealed or end-capped with septa, and stored
away from light in its original box.
Troubleshooting Installation Problems

More often than not, GC column problems are traceable to something improperly done during installation. For a more complete
treatment of the subject, ask for your FREE guidebook GC Troubleshooting.
High Performance Operation Recommendation Before Use:
This column should be conditioned for at least 4 hours at its
maximum isothermal temperature OR at 20 C above the
maximum temperature of the method, whichever is less.
Zebron GC Columns MS Certification

Simply because GC-MS is one of the most important and rapidly
growing techniques, many manufacturers have designated these
columns MS Certified in order to draw attention to them. Designed
to bleed less at higher temperatures, these columns can analyze a
broad range of compounds at lower levels than ever before.
Now anyone doing trace analysis can directly transfer their

method to a true low bleed, high-temperature column without
virtually any selectivity changes. Zebron is the clear choice.
To provide such columns, some manufacturers high-grade or

select the best-performing capillary columns from batches of their
standard columns and label these MS grade. Other manufacturers
modify the chemistry of the polymer backbone (with, e.g.,
silphenylene) in order to make lower bleed at higher temperature
limits possible. This last approach can change selectivity, however,
which then leads to problems when the method is upgraded from a
traditional to the new MS-certified column.
Column Bleed Comparison Test by MS Detection
Phenomenex MS-certified Zebron columns, however, are neither

hand-selected nor will they change selectivity. Instead, they offer
excellent thermal performance with identical selectivity compared
to traditional non-MS columns. Every column is manufactured
to provide very high levels of batch-to-batch and column-tocolumn reproducibility, along side some of the most exacting bleed
specifications in the industry your assurance of a quality column
that will perform for demanding applications.

Injection: 225 C with a constant flow of 1.1 mL/min of helium
Oven Program: 240 C for 10 min to 280 C at 40 /min for 10 min to
320 C at 40 /min for 10 min to 340 C at 40 C/min
for 10 min to 360 C at 40 C/min for 10 min
Detector: MS, Scan range: 36-450 amu
360 C
4500
Thousands
GC COLUMN INSTALLATION INSTRUCTIONS / MS CERTIFICATION
GC Column Installation Instructions
4000
3500
3000
1 Zebron ZB-5
2500
340 C
Equivalent column from leading competitors:
2000
2 Varian FactorFour , VF-5ms
3 J&W DB-5ms
4 Agilent HP-5ms
5 Restek Rtx-5Sil MS
3
2
1
320 C
280 C
240 C
0
10
20
30
40
50
pA
1500
1000
500
0
60 min
Testing conditions were similar for all columns. All columns were new and obtained from the original
manufacturer or its authorized channels. Data provided by Phenomenex in-house evaluations and
represents bleed values obtained after 2 hours of conditioning.
Phenomenex
Hplc Calculations
Column Efficiency
In general, N = Number of Theoretical Plates, a is a constant
depending on method used, tR = retention time of peak, and
W = the peak width at a given peak height.
N=a
tR
(W)
Adjusting Flow Rate for Different Column IDs

When scaling up from analytical to preparative mode or when scaling down from analytical to microbore LC, it is often desirable to
keep retention times constant. The flow rate can be adjusted so that
the columns operate at the same linear velocity.
When switching from a column with a radius (0.5 x ID) of r1 to another with a radius of r2, the flow rate must be altered by a factor
of X, where:
Method
X = (r2/r1)2
Peak Width Peak Height

Peak Width at 4.4 % Peak Height (5s method)
Tangential (ca. 13.5 %)
5.54
25
16
The peak width at height is the most commonly used method

for calculating HPLC column efficiency.
For example, when scaling up from a 250 x 4.6 mm column to a 250

x 10 mm ID column, the flow rate must be increased by a factor of
4.73 in the 10 mm column to generate the same linear velocity as
that of the 4.6 mm ID column, as derived below:
X = (5.0/2.3)2 = 4.73
Inject
The general formula which will convert flow rate from any given
column dimension to any other is as follows:
Peak width at
half height (W1/2)
is actual width of
peak at half-height
W1/2
F2 = F1 x (L2/L1) x (r2/r1)2
Tangent lines
are used in
determining
Peak width
at base (W)
10 % peak
height
Retention time for:

unretained peak
t0
tR1
Column Efficiency
NW1/2 = 5.54
peak 1
tR2
(W / )
tR
A = peak from @ 10 % to dropline

B = dropline to peak tail @ 10 %
peak 2
Peak Asymmetry
AS =
1 2
B
A
Peak Asymmetry
AS = B/A at 10 % peak height
Capacity Factor
(also known as Retention Factor or Relative Retention)
k = (tR-t0)/t0
L = length of the column, in mm

r = radius of the column, in mm
F = flow rate, in mL/min
1 designates the first, or reference, column
2 designates the second column
Effect of Different Conditions on Sample Retention

Change in Separation
Flow rate
Column volume
Increase in percentage
of strong solvent
New strong solvent
pH value
Column packing
(e.g., cyano vs. C18)
Increase temperature
New mobile phase additives
Effect on Retention Time:

t0
Run Time
Band Spacing
F
Vm
None
1/F
Vm
Decrease
None
None
Small change
None
None
Little
Changes
Changes
Changes
Changes
Changes
Changes
None
None
Decrease
Changes
Small change
Changes
Applications of Liquid Chromatography
Where tR is the elution time of retained component, and t0 is the

elution time of the unretained sample.
Separation Factor
(also known as Selectivity)
HPLC CALCULATIONS
The Capacity Factor, k, of a sample component is a measure of the

degree to which that component is retained by the column relative
to an unretained component (such as uracil).
Where:

Appendices
The selectivity parameter, , is a measure of the spacing between

two peaks and is expressed as:
a=k2/k1
Resolution
Rs, defined as the amount of separation between two adjacent
peaks, is given by:
k
Rs = N a-1
4

k+1
( )( )
where k is the average value for the two peaks.
Technical information found in this Appendices

can also be viewed on our website. Please visit
www.phenomenex.com/chromtips.
Phenomenex
(From: D.L. Saunders, in Chromatography, 3rd ed, E. Heftmann, Ed., p. 81, Van Nostrand Reinhold:
New York, 1975. With permission.)
383
Chromatographic Parameters
Parameters
Unit
Symbols
Kirkland et al.*
ASTME E-19**
Chromatographia**
Retention time of an unretained solute
t0
t'M
tm
Retention time, measured from the start
tR
tR
tm + s
Reduced retention time
t'R = tR - t0
t'R = tR - tM
ts = tm + s - tm
Band width
yt
wb
Capacity factor (Retention factor)
Selectivity factor
Resolution
k=
t'R
tO
= k2 = t'R2
k1
t'R1
Rs=2
Number of theoretical plates
N = 16
Column length
cm
Height equivalent of a theoretical plate (plate height)
cm
H=
cm s-1
u=
Linear velocity of the mobile phase
k=
rji =
t'R2 - t'R1
w2 + w1
( )
tR
Rji=2
N
L
to
t'Rj
H=
u=
r=
tm
t"S
t'S
t'i
ts
k=
tM
n = 16
L
t'R
tRj - tR1
ytj + yti
( )
tR
y1
L
n
L
tm
Rs=2
n = 16
t"m+s - t'm+s
w"b + w'b
( )
tm+s
wb
h=
u=
L
n
L
tm
Appendices
| CHROMATOGRAPHIC PARAMETERS / reversed phase method development
*Modern Practice of Liquid Chromatography, Ed. J.J. Kirkland, Wiley, New York (1971).
**B. Versino and F. Geib, Supplement in: Chromatographia 3 (1970).
384
Reversed Phase Method Development

Amounts of Sample That Can Be Separated
Column Type
Capillary (Fused Silica)

Microbore
Analytical
Semi-Prep
Preparative
Approx.Dead Typical Flow

Typical and (Max.)
Typical and (Max.)
ID (mm) Volume (mL)* Rate (mL) Injection Masses (mg) Injection Volumes (L)**
0.32
1.0
4.6
10.0
20.0
0.0075
0.07
1.5
7.3
29.2
0.001 - 0.02
0.02 - 0.1
0.5 - 2.0
5.0 - 20
10 - 200
0.001 (0.01)
0.01 (0.1)
0.1 (2.5)
1.0 (25)
5.0 (500)
1 (10)
5 (25)
10 (200)
50 (1000)
200 (5000)
*The column Dead Volume (Vo) may be estimated from:

Column Dead Volume (mL) = Vo = 0.487 x d2 x L
Where: L = column length (cm); 15 cm (150 mm) used for calculation.
d = column ID (cm, not mm)
**The maximum allowable Sample Injection Volume (Vi) can be estimated as
Vr
follows: Maximum Injection Volume =Vi =
2N
Where: Vr = the retention volume of the first peak (mL)
N = number of theoretical plates per column
Column Loading Capacity

Retention time and peak width are independent of the amount of
sample injected up to a point called the column capacity (). Above
this point, retention times (k) decrease and peak widths increase.
When retention decreases by 10% of its normal value, the column
capacity has been exceeded. Increases in peak width can cause
overlap with adjacent peaks, reducing the purity of collected fractions. Analytical scale
columns have capaci10 %
ties on the order of 1
mg, while preparative
scale columns can separate tens of milligrams
or even grams depending on the diameter of
the column.
Probes for Column

Characterization
The following tests are not 100 % accurate for
column characterization and it should be noted
that there will be exceptions where a column
gives a false value caused by other interaction
mechanisms with the stationary phase and analyte
probe.
Hydrophobicity
Polarity
H-bonding
Aromatic Selectivity
Silanol Activity
Tested by k butylbenzene
Tested by k caffeine
Tested by (k caffeine/k phenol)
An estimate of ligand selectivity by - interaction
Tested by (k benzylamine/k phenol)
Viscosity of Solvent Mixtures

as a Function of Composition
Phenomenex

Factors Controlling Resolution
The equation below shows how resolution is affected by the
controllable factors: Selectivity, Efficiency and Retention:
Rs = N
4
k
( a-1
a )( k+1 )
Goal
Factor
Selectivity Factor
= k2 / k1
(alpha) = k1 / k2. For closely spaced peaks, the alpha value is close to 1.0, so small
changes in alpha have large effects on the resulting resolution.
Control
LOG
NEFF
Improve selectivity () by altering the composition of the mobile phase or stationary

phase. pH and temperature are two other potential variables to control, if appropriate.
RS = 1.5
RS = 2.0
10 m
van Deemter Plot

Efficiency Factor
N = -8 ln(f) (tr / wf)2
Since resolution is a function of the square root of N, large changes in N are required to
make small changes in resolution. Increasing efficiency is often an overrated method
of improving resolution.
5 m
C
H=A+
Improve efficiency (N) by increasing column length, decreasing particle size of column
packing, or decreasing flow rate. Minimize extra-column dead volume.
This is a function of k, the average retention factor for 2 adjacent bands. When k
is small (0-1), this factor severely limits resolution. Larger values of k will improve
resolution, yet increase associated retention times. Above k = 20, the amount of
improvement is small.
B
20
30
flow velocity
RS
Improve k by changing the eluent strength.
Retention factor = (tr-to) / to (previously known as capacity factor)

Fractional height of peak, e.g., 0.5, 0.1
Width of peak at fractional height f
Elution time of the retained component
Elution time of an unretained component (one that elutes in the void volume)
Height equivalent to a theoretical plate (HETP) = L , or as defined by the terms of the van Deemter plot above
N
Eddy diffusion
Longitudinal diffusion
Mass transfer kinetics of the analyte between mobile and stationary phase
Linear velocity
Appendices
=
=
=
=
=
=
=
=
=
=
Where: k

f

Wf

tr

to

H

A

B

C

u
2 - 10 (optimum)
Retention Factor
k = (tr-t0) / t0
10
B
+ Cu
u
A

Phenomenex
385
HPLC Column Protection

Upon Receipt Of The Column
Verify the column you received is the column you ordered
Storing The Column

Column storage conditions affect column lifetime
Check the column for physical damage that may have occurred during
shipping
Never store columns containing buffers or ion-pairing reagents
Test the column immediately to verify quality and performance
Flush with at least five column volumes of mobile phase without buffer
to remove any buffers or salts
All columns are shipped in the testing solvent, unless otherwise specified
Storage Conditions for Silica-Based HPLC Columns
Each Phenomenex manufactured HPLC column is individually

packed and tested to ensure outstanding column quality. Every
column is supplied with its Test Chromatogram and a Specification Sheet that indicates testing conditions, operating parameters,
column serial number and identity.
The warranty period begins upon receipt of the column. Testing is
especially important if the column is to be placed in storage. Test
the column using the same conditions in the test chromatogram.
Use the formulas on p. 383 to determine column efficiency and
peak asymmetry.
Chromatographic performance depends on the entire system, not
just the column. Columns are QC tested using optimum conditions
to minimize band-spreading from Extra Column Effects. See pp.
365-366 for HPLC Column Performance Check Standards. Most
variations from the Phenomenex test data are due to extra column
effects created by your systems design (e.g., injector, flow cell,
connecting tubing, etc.). If you have any questions regarding your
test results or the column quality, or if there are signs of damage,
contact your local distributor or Phenomenex immediately.
Mobile Phase Considerations
Use only HPLC grade solvents
Make sure solvents are miscible (see Table p. 387)

Always check sample solubility
Stationary Phase Considerations
Maintain pH between 2.0 and 8.0**
Use presaturator columns and guard columns
Avoid aldehydes and ketones with amino columns
**Consult Phenomenex for columns that have extended pH ranges.
Backpressure and Flow Rates

Keep backpressures below 3500 psi (245 bar), unless otherwise specified
Avoid any sudden pressure changes
If high backpressure is observed reverse flush the column
(do not try this on other manufacturers columns)
Appendices
HPLC COLUMN PROTECTION
If possible, use the mobile phase as the diluent (sample solvent)
Use a backpressure regulator if you are experiencing out-gassing problems

in the detector cell
Columns can be operated at any flow rate that is consistent with
the backpressure limitations described below. Flow rates should be
optimized to provide the highest efficiency for your sample.
Typical Column Flow Rates & Backpressures

3
5
5
5
5
5
10
10
*column length
386
Typical Flow Rate

(mL/min)
4.6
1.0
2.0
3.0
4.6
10.0
4.6
21.2
0.5
0.05
0.2
0.5
1.0
5.0
2.0
20.0
65 % Acetonitrile/
35 % Water
Isopropanol or Hexane
Methanol*
0.05 % NaN3 in Water or
10 % Methanol
80 % Acetonitrile/
20 % Water
*Flush column with 50 mL water prior to storage solvent
Column Cleaning Procedures

Due to interactions between the stationary phase and sample
components, HPLC columns may occasionally require cleaning or
regeneration. The following conditions apply to Phenomenex silicabased columns with the exception of chiral columns.
Flow rates should be 1/5 - 1/2 of the typical flow rate
To estimate the column volume, use the following equation:
V = column volume in mL
V=r L
2
r = column radius in cm
UNBONDED SILICA COLUMNS (Si)

Rinse with 10 column volumes each of:
Hexane
Methylene Chloride
Isopropanol
Methylene Chloride
Mobile Phase
Water Removal: Flush column with 30 mL
2.5 % 2,2-dimethoxy propane and 2.5 %
glacial acetic acid in hexane.
Degas and filter all mobile phases prior to use
Internal
Diameter(mm)
Storage Solvent
Reversed Phase
C18, C12, C8, C4, C2, C1, Phenyl, PFP
Normal Phase
Silica, CN, NH2, PAC, Diol, Alumina
Ion-Exchange
SAX, SCX, WAX, WCX
Size Exclusion
Diol
HILIC
Luna HILIC
L = column length in cm
Use only highest purity chemicals and reagents
Particle
Size (m)
Column Type
Typical Pressure (psi)

150 mm*
250 mm*
985
750
750
732
710
750
355
170
1640
1250
1250
1226
1180
1250
590
280
REVERSED PHASE COLUMNS (C18, C12, C8,

C4, C2, C1, PHENYL, PFP, CN, NH2)
95 % Water/5 % Acetonitrile
(for buffer removal)
THF
95 % Acetonitrile/5 % Water
Mobile Phase
REVERSED PHASE PROTEIN/PEPTIDE
COLUMNS (C18, C8, C5, C4, Phenyl)
Rinse with 20 column volumes of mobile
phase with buffer removed.
Run gradient (2x):
(A) 0.1 % aqueous TFA in water
(B) 0.1 % TFA in Acetonitrile/Isopropanol (1:2)
25 % B to 100% B for 30 minutes
Equilibrate with 10 column volumes of
mobile phase.
Bonded Normal Phase Columns
(CN, NH2, DIOL, PAC)
Chloroform
Isopropanol
Methylene Chloride
Mobile Phase
Exception: Recommended for cleaning Luna
Amino when used in reversed phase mode:
1. Wash with at least 30 column volumes of
Sodium Hydroxide pH 11.0
2. Flush with at least 30 column volumes of
water (HPLC grade)
3. Re-equilibrate to mobile phase conditions.
GFC/SEC COLUMNS FOR PROTEINS

BIOSEP-SEC-S (300 x 7.8 mm size columns)
Rinse with 5 column volumes of:
0.1 M Phosphate buffer pH 3.0.
For strongly retained proteins:
Run 100 % Water to 100 % Acetonitrile to
100 % Water over 60 minutes OR wash with
5 column volumes of SDS or 6 M Guanidine
Thiocyanate or 10 % DMSO.
ION-EXCHANGE COLUMNS
(SAX, SCX, NH2, WAX, WCX)
500 mM Phosphate Buffer pH 7
10 % Acetic Acid (Aq)
5 column volumes of Water
10 column volumes of Phosphate Buffer pH 7
10 column volumes of Methanol
For protein removal, follow the above
procedure with this exception:
Substitute 10 column volumes of Methanol
with 10 column volumes of 5 M Urea or 5 M
Guanidine Thiocyanate.
HILIC

(for buffer removal)
95 % 100 mM Ammonium Acetate,

pH 5.8 / 5% Acetonitrile
Mobile Phase
HPLC columns running

water-free, flammable organic
solvents (e.g., normal phase,
chiral, GPC) can generate
static electricity and should
be properly grounded to
avoid a potentially dangerous
electrical discharge.
Phenomenex
Solvent Miscibility Table

Solvent Miscibility Table
Boiling
Point
(C)
(% w/w)
Relative
Polarity
R-H
Alkanes
Ar - H
R-O-R
Aromatics
Ethers
R-X
Alkyl halides
R - COOR
R - NH2
Esters
Aldehydes
and ketones
Amines
R - OH
Alcohols
R - COHN2
R - COOH
H - OH
Amides
Carboxylic acids
Water
R - CO - R
Representative Solvent
Compounds
Petroleum ethers,
ligroin, hexanes
Toluene, benzene
Diethyl ether
Tetrachloromethane,
chloroform
Ethyl acetate
Acetone, methyl ethyl ketone
Pyridine, triethylamine
Methanol, ethanol,
isopropanol, butanol
Dimethylformamide
Ethanoic acid
Water
Appendices
Polar
Group
Increasing Polarity
Nonpolar
Compound
Formula
SOLVENT MISCIBILITY TABLE / Solvent Polarity Chart
Solvent Polarity Chart

Phenomenex
387
a selection of
HPLC Material Sorbent Characteristics

This selection is, neither in terms of manufacturers nor in terms of their products, a complete list and the accuracy of data is not guaranteed.
Phenomenex Sorbents
Appendices
Packing Material

Aeris WIDEPORE C4
Aqua C18
Aqua C18
Bondclone Silica
Bondclone C18
Clarity Oligo-RP
Clarity Oligo-WAX
Clarity Oligo-MS
Columbus C8
Columbus C18
Gemini C18
Gemini C6-Phenyl
Gemini-NX C18
HyperClone BDS C8
HyperClone BDS C18
HyperClone MOS (C8)
HyperClone ODS (C18)
HyperClone CN (CPS)
IB-Sil C18
IB-Sil C8
IB-Sil CN
Jupiter C4
Jupiter C5
Jupiter C18
Jupiter Proteo
Kinetex C18
Kinetex XB-C18
Kinetex PFP
Kinetex C8
Kinetex HILIC
Luna PFP(2)
Luna Phenyl-Hexyl
Luna Silica(2)
Luna C5
Luna C8
Luna C8(2)
Luna C18
Luna C18(2)-HST
Luna C18(2)
Luna CN
Luna HILIC
Luna NH2
Luna SCX
Maxsil Silica
Maxsil C8
Maxsil C18
Onyx C18
PhenoSphere Si
PhenoSphere C1
PhenoSphere C6
PhenoSphere C8
PhenoSphere ODS (1)
PhenoSphere ODS (2)
PhenoSphere CN
PhenoSphere Phenyl
PhenoSphere SCX
PhenoSphere SAX
PhenoSphere CN (2)
PhenoSphere-NEXT Silica
Particle
Shape/Size
(m)
Pore
Size
()
Pore
Volume
(mL/g)
Surface
Area
(m2/g)
Carbon Load
%
Calculated*
Bonded Phase
Coverage
(mole/m2)
End
Capping
pH
Range
USP
Packing
Core-Shell 3.6
Core-Shell 3.6
Core-Shell 3.6
Core-Shell 1.7, 3.6
Spher. 3, 5, 10, 15
Spher. 5, 10, 15
Irreg. 10
Irreg. 10
Spher. 3, 5, 10
Spher. 10
Core-shell 1.7, 2.6
Spher. 5
Spher. 5
Spher. 3, 5, 10
Spher. 3, 5
Spher. 3, 5, 10
Spher. 3, 5
Spher. 3, 5
Spher. 3, 5
Spher. 3, 5
Spher. 3, 5
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 5, 10, 15
Spher. 5, 10, 15
Spher. 5, 10, 15
Spher. 4, 10
Core-shell 1.7, 2.6
Core-shell 1.7, 2.6
Core-shell 1.7, 2.6
Core-shell 1.7, 2.6
Core-shell 1.7, 2.6
Core-shell 1.7, 2.6
Spher. 3, 5
Spher. 3, 5, 10, 15
Spher. 3, 5, 10, 15
Spher. 5, 10
Spher. 5, 10
Spher. 3, 5, 10, 15
Spher. 5, 10
Spher. 2.5
Spher. 3, 5, 10, 15
Spher. 3,5,10
Spher. 3, 5
Spher. 3,5,10
Spher. 5,10
Irreg. 5, 10
Irreg. 5, 10
Irreg. 5, 10
C18 Bonded Rod**
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 5, 10
Spher. 5, 10
Spher. 3, 5, 10
Spher. 3, 5
100
125
200
148
148
110
360
100
110
110
110
110
110
130
130
120
120
120
125
125
125
300
300
300
90
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
200
100
100
65
65
65
130*
80
80
80
80
80
80
80
80
80
80
80
120
1.05
1.15
1.1
1.1
0.6
0.6
0.6
0.6
0.6
0.75
0.75
0.75
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
1.0
0.7
0.7
0.7
1.0
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
25
25
25
200
320
215
300
300
375
150
200
375
375
375
375
375
155
155
155
155
155
165
165
165
170
170
170
475
200
200
200
200
200
200
400
400
400
440
440
400
440
400
400
400
200
400
400
500
500
500
300
220
220
220
220
220
220
220
220
220
220
220
380
10
15
11
0
10, Monomeric
14
12
13
19
14
12
14
7
11
6.5
10
4
11, Monomeric
7.5, Monomeric
4.5, Monomeric
5.0
5.5
13.34
15
12
10
9
8
0
8
11.5
17.5
0
12.5
14.75
13.5
19
17.5
17.5
7.0
5.7
9.5
0.55 % Sulfur Load
0
6.5, Monomeric
12.5, Monomeric
18
0
4, Monomeric
6, Monomeric
6, Monomeric
7, Monomeric
12, Monomeric
4, Monomeric
3.5, Monomeric
6, Monomeric
4, Monomeric
3.5, Monomeric
0
1.61
0.8 meq/g
3.27
4.29
4.15
6.30
5.30
5.50
2.20
4.00
7.85
5.50
5.50
3.00
3.00
3.00
3.80
4.30
5.80
0
1.20
1.25
3.6
0
1.08
2.27
3.54
1.74
2.50
2.82
1.10
0.4 meq/g
0.6 meq/g
2.50
Yes
Yes
Yes
Yes
Proprietary
Proprietary
No
Yes
Yes
Yes
Yes
Double
Double
Yes
Yes
Yes
Yes
Yes
Yes
Yes
No
Yes
Yes
No
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
No
Yes
Yes
Yes
No
Yes
Yes
Yes
Yes
Yes
Yes
Yes
No
No
No
No
No
Yes
Yes
No
No
Yes
Yes
Partial
Yes
Partial
Partial
No
No
Partial
No
1.5-9
1.5-9
1.5-9
1.5-9
2.5-7.5
2.5-7.5
2.5-7.5
1-12
1-11
1.5-10
2.5-7.5
2.5-7.5
1.0-12.0
1.0-12.0
1.0-12.0
2.0-7.5
2.0-7.5
2.0-7.5
2.0-7.5
2.0-7.5
2.5-7.5
2.5-7.5
2.5-7.5
1.5-10
1.5-10
1.5-10
1.5-10.0
1.5-8.5
1.5-8.5
1.5-8.5
1.5-8.5
2.0-7.5
1.5-8.5
1.5-9.0
1.5-9.0
2.0-7.5
1.5-9.0
1.5-9.0
1.5-9.0
1.5-9.0
1.5-9.0
1.5-9.0
1.5-7.0
1.5-8.0
1.5-11.0
2.0-7.0
2.5-7.5
2.5-7.5
2.0-7.5
2.5-7.5
2.5-7.5
2.5-7.5
2.5-7.5
2.5-7.5
2.5-7.5
2.5-7.5
2.5-7.5
2.5-7.5
2.5-7.5
L1
L7
L26
L1
L1
L1
L3
L1
L8
L1
L7
L1
L1
L11
L1
L7
L1
L7
L1
L10
L1
L7
L10
L26
L1
L1
L1
L43
L7
L3
L11
L43
L11
L3
L7
L7
L1
L1
L1
L10
L20
L8
L3
L7
L1
L1
L3
L13
L15
L7
L1
L1
L10
L11
L14
L10
L3
Effective Carbon Load. ** Mesopore size listed. Macropore size is 2 m. pH range is 1.5-10 under isocratic conditions. pH range
is 1.5-8.5 under gradient conditions. pH range is 1.5-10 under isocratic conditions. pH range is 1.5-9.0 under gradient conditions.
388
Phenomenex
a selection of

This selection is, neither in terms of manufacturers nor in terms of their products, a complete list and the accuracy of data is not guaranteed.
Phenomenex Sorbents (cont'd)

Particle
Shape/Size
(m)
Pore
Size
()
Pore
Volume
(mL/g)
Surface
Area
(m2/g)
Spher. 3, 5
Spher. 3, 5
Spher. 3, 5
Spher. 5
Spher. 3, 5, 7, 10, 15
Spher. 5
Spher. 5
Spher. 3, 5, 10
Spher. 5
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 3, 5, 10
Spher. 2.5
Spher. 2.5
Spher. 2.5
Spher. 2.5
Spher. 4, 10
Spher. 4, 10
Spher. 4, 10
Spher. 4, 10
Spher. 5
Spher. 3, 5, 7
Spher. 5, 7
120
120
120
120
100
150
150
100
100
80
80
80
80
80
80
80
100
100
100
100
80
80
80
80
60
90
60
1.1
1.1
1.0
1.05
1.05
1.05
1.05
0.80
0.75
0.80
380
380
380
380
410
310
310
450
450
200
200
200
200
200
200
200
400
400
400
400
475
475
475
475
550
370
550
Packing Material
PhenoSphere-NEXT C8
PhenoSphere-NEXT C18
PhenoSphere-NEXT CN
PhenoSphere-NEXT Phenyl
PolymerX RP-1
Prodigy ODS(2)
Prodigy C8
Prodigy ODS (3)
Prodigy Phenyl (PH-3)
SphereClone Silica
SphereClone C6
SphereClone C8
SphereClone ODS (1)
SphereClone ODS (2)
SphereClone NH2
SphereClone SAX
Synergi Fusion-RP
Synergi Max-RP
Synergi Hydro-RP
Synergi Polar-RP
Synergi Fusion-RP
Synergi Max-RP
Synergi Hydro-RP
Synergi Polar-RP
Ultracarb C8
Ultracarb ODS (20)
Ultracarb ODS (30)
Carbon Load
%
10
14
8
11
0
18.5,
12.6,
15.5,
10.0,
6
6
7
12
2
12
17
19
11
12
17
19
11
14,
22,
31,
Monomeric
Monomeric
Monomeric
Polymeric
Monomeric
Monomeric
Monomeric
Calculated*
Bonded Phase
Coverage
(mole/m2)
End
Capping
pH
Range
USP
Packing
N/A
3.50
5.00
3.21
2.45
3.15
2.71
3.53
4.06
Yes
Yes
Yes
Yes
No
Yes
Yes
Yes
No
No
Yes
Yes
Partial
Yes
No
No
Yes
Yes
Proprietary
Proprietary
Yes
Yes
Proprietary
Proprietary
Yes
Yes
Yes
2.5-7.5
2.5-7.5
2.5-7.5
2.5-7.5
0-14
2.0-9.0
2.0-9.0
2.0-9.0
2.0-9.0
2.5-7.5
2.5-7.5
2.5-7.5
2.5-7.5
2.5-7.5
2.5-7.5
1.5-9.0
1.5-9.0
1.5-7.5
1.5-7.0
1.5-9.0
1.5-9.0
1.5-7.5
1.5-7.0
2.5-7.5
2.5-7.5
2.5-9.0
L7
L1
L10
L11
L21
L1
L7
L1
L11
L3
L15
L7
L1
L1
L8
L1
L1
L1
L1
L11
L7
L1
L1
*As per Sander, L.C., and Wise, S.A., Anal. Chem. 1984, 56, 504-510,
10 6Pc
1200nc - Pc(M-1)
1
S
and Pc= percent carbon of bonded phase, nc is the number of carbon atoms in the bonded silane molecule,
M is the molecular weight of the bonded silane molecule, and S is the specific surface area of the bonded silica in m2/g.
NOTE: Phenomenex has not verified above values experimentally, and does not guarantee their accuracy. Above specifications subject to change without prior notice.
pH range is 1.5-10 under isocratic conditions. pH range is 1.5-9.0 under gradient conditions.
Non-Aqueous SEC/GPC Materials
Aqueous SEC/GFC Materials

Exclusion
Limit***
Phenogel 50
Phenogel 100
Phenogel 500
Phenogel 103
Phenogel 104
Phenogel 105
Phenogel 106
Phenogel Linear
Phenogel MXL
Phenogel MXM
Phenogel MXH
Spher. 5, 10, 20
Spher. 5, 10, 20
Spher. 5, 10, 20
Spher. 5, 10, 20
Spher. 5, 10, 20
Spher. 5, 10, 20
Spher. 5, 10, 20
Spher. 5, 10, 20
Spher. 5, 10
Spher. 5, 10
Spher. 5, 10
50
100
500
103
104
105
106
Mixed
Mixed
Mixed
Mixed
3 x 10
6 x 103
1 x 104
7 x 104
5 x 105
1 x 106
1 x 107
1 x 107
1 x 105
5 x 105
1 x 107
3
Packing
Material
BioSep-SEC-S 2000
BioSep-SEC-S 3000
BioSep-SEC-S 4000
PolySep-GFC-P 1000
PolySep-GFC-P 2000
PolySep-GFC-P 3000
PolySep-GFC-P 4000
PolySep-GFC-P 5000
PolySep-GFC-P 6000
PolySep-GFC-P Linear
Particle Shape/Size
(m)
Pore Size**
()
Exclusion
Limit***
Spher. 5
Spher. 5
Spher. 5
Spher.
Spher.
Spher.
Spher.
Spher.
Spher.
Spher.
145
290
500
N/A
N/A
N/A
N/A
N/A
N/A
N/A
3 x 105
7 x 105
1 x 106
2 x 103 (PEG)
9 x 103 (PEG)
50 x 103 (PEG)
20 x 104 (PEG)
20 x 105 (PEG)
10 x 106 (PEG)
10 x 107 (PEG)
Appendices
Pore Size**
()
Packing Material
Particle Shape/Size
(m)
where N(mol/m2) =
**Pore Size is expressed in Angstroms (10-10 meters). This is actually a convention used by manufacturers to indicate the approximate molecular weight of compounds that can be separated on a given SEC
packing; these values do not indicate the actual size (diameter) of the pores on the surface of the particle.
***Exclusion Limit is expressed in Daltons (the molecular weight) of the specified compound excluded from the pores of the base material. Practically speaking however, the exclusion limit is more accurately
a reflection of the hydrodynamic volume occupied by the solvated compound.
For material sorbent characteristics of other HPLC columns manufactured and sold by
Phonemenex, please visit the Web link http://www.phenomenex.com/chromtips
Phenomenex
389
Appendices
CHROMATOGRAM INDEX
Chromatogram Index
GC
GC
GC
Acenaphthalene....................................................... 69, 84, 117
ASTM Method D 5060.......................................................... 142
Bromochloroacetic acid....................................................... 120
Acenaphthene..................................69, 84, 115, 116, 117, 134
ASTM Method D 5135.................................................. 134, 141
Bromochloromethane.................................................. 123, 136
Acenaphthylene........................................... 115, 116, 117, 134
ASTM Method D 5441.......................................................... 142
Bromodichloroacetic acid..................................................... 120
Acetal.................................................................................. 130
ASTM Method D 5501.................................................... 91, 142
Bromodichloromethane........................................................ 123
Acetaldehyde................................................... 89, 95, 127, 130
ASTM Method D 6584.......................................................... 143
Bromofluorobenzene, 4-...................................................... 123
Acetaminophen.............................................................. 82, 127
Atraton................................................................ 119, 121, 122
Bromoform.......................................................................... 123
Acetic acid........................................................................... 131
Atrazine............................................................... 119, 121, 122
Bromomethane.................................................................... 123
Acetic acid methyl ester (methyl acetate)............................. 135
Azinphos ethyl..................................................... 119, 121, 122
Bromo-2-nitrobenzene, 1-............................................. 87, 118
Acetic acid-1-methyl ethyl ester.......................................... 135
Azinphos methyl.................................................. 119, 121, 122
Bromopheniramine........................................................ 82, 127
Acetone..................................95, 123, 127, 131, 134, 135, 141
Azobenzene................................................................. 115, 116
Bromophenylphenylether, 4-........................................ 115, 116
Acetonitrile...................................124, 128, 129, 133, 135, 138
Barbiturates......................................................................... 126
BTEX............................................................................. 88, 134
Acetophenone...................................................... 115, 116, 127
BDE isomers........................................................................ 117
Butachlor............................................................. 119, 121, 122
Acetylaminofluorene, 2-............................................... 115, 116
Beer.................................................................................... 282
Butalbital............................................................... 82, 126, 127
Acifluorfen........................................................... 120, 121, 122
Behenic Acid.................................................................. 90, 131
Butanal................................................................................ 137
Acrolein............................................................................... 123
Benactyzine........................................................................... 82
Butane......................................................................... 141, 142
Acrylamide.......................................................................... 124
Benefin................................................................ 119, 121, 122
Butanediol, 2,3-..................................................................... 98
Acrylonitrile......................................................................... 123
Bentazon............................................................................. 120
Butanetriol........................................................................... 143
ALA............................................................................. 281, 282
Bentazon (methyl ester)............................................... 121, 122
Butanol........................................................................ 135, 136
Alachlor......................................................... 92, 119, 121, 122
Benzaldehyde...................................................................... 135
Butanol, 1-................................................................... 134, 141
Alcohols............................................................................... 136
Benz[a]anthracene............................69, 84, 115, 116, 117, 134
Butanol, 2-..............................................95, 127, 134, 136, 141
Aldehydes............................................................................ 137
Benzene....85, 88, 123, 128, 129, 133, 134, 135, 140, 141, 142
Butanol, n-........................................................................... 136
Aldrin................................................................. 80, 87, 92, 118
Benzenepropanol................................................................. 136
Butanol, sec-............................................................... 136, 141
Aliphatic amines.................................................................. 138
Benzidine............................................................. 115, 116, 138
Butanol, tert-..........................95, 123, 127, 135, 136, 141, 142
Aliphatic hydrocarbons........................................................ 142
Benzocaine.......................................................................... 127
Butanone (MEK), 2-...................................... 123, 124, 135, 137
Allobarbital.................................................................... 82, 127
Benzodiazepines.................................................................. 127
Butter.................................................................................... 79
Allyl alcohol (2-propen-1-ol)........................................ 135, 136
Benzo[b]fluoranthene........................69, 84, 115, 116, 117, 134
Butyl acetate....................................................................... 135
Alprazolam.......................................................................... 127
Benzo[j]fluoranthene...................................................... 84, 117
Butyl alcohol, n-................................................................... 141
Ametryn............................................................... 119, 121, 122
Benzo[k]fluoranthene........................69, 84, 115, 116, 117, 134
Butylamine, n-..................................................................... 138
Aminobiphenyl, 4-....................................................... 115, 116
Benzo[c]fluorene.................................................................... 84
Butylamine, sec-.................................................................. 138
Ammelide............................................................................ 133
Benzoic acid...................................76, 115, 116, 124, 136, 137
Butylamine, tert-.................................................................. 138
Ammeline............................................................................ 133
Benzo[g,h,i]perylene.........................69, 84, 115, 116, 117, 134
Butylate............................................................... 119, 121, 122
Amobarbital........................................................... 82, 126, 127
Benzo[a]pyrene.................................69, 84, 115, 116, 117, 134
Butylbenzene............................................................... 141, 143
Amorphene, -.................................................................... 132
Benzothiophenes................................................................. 143
Butylbenzene, n-.................................................................. 123
Amphetamines.............................................................. 94, 125
Benzoyl alcohol.................................................................... 116
Butylbenzene, sec-.............................................. 123, 134, 141
Amyl methyl ether, tert-....................................... 123, 141, 142
Benzoylecgonine.................................................................. 125
Butylbenzene, tert-.............................................. 123, 134, 141
Aniline....................................................86, 115, 116, 136, 138
Benzphetamine.............................................................. 82, 127
Butyl benzyl phthalate.................................................... 75, 133
Anisole................................................................................ 135
Benzyl acetate..................................................................... 132
Butyl ethyl ether, tert-.................................................. 141, 142
Anthracene...............................69, 84, 115, 116, 117, 131, 134
Benzyl alcohol............................................................. 115, 136
BZ# isomers.......................................................................... 81
Aprobarbital................................................................... 82, 127
Benzylamine.................................................................. 86, 138
Cadinene............................................................................. 132
Arabinose, D-(-)-.................................................................. 132
Benzyl benzoate.................................................................. 132
Caffeine......................................................................... 82, 127
Arachidic acid................................................................ 90, 131
Benzyl butyl phthalate.................................................. 115, 116
Campesta-3,5-diene............................................................ 131
Arachidonic acid methyl ester.............................................. 130
Benzyl salicylate.................................................................. 132
Campesterol........................................................................ 131
Aramite........................................................................ 115, 116
BHC, -............................................................. 80, 87, 92, 118
Camphene........................................................................... 132
Aroclor........................................................................... 81, 124
BHC, -.............................................................. 80, 87, 92, 118
Camphor.............................................................................. 132
Aromatic amines............................................................ 86, 138
BHC, -.............................................................. 80, 87, 92, 118
Captafol................................................................................. 92
Aromatic solvents................................................................ 141
BHC (Lindane), -............................................... 80, 87, 92, 118
Captan................................................................................... 92
Aromatics............................................................................ 134
Bioethanol..................................................................... 91, 142
Carbofenothion.................................................... 119, 121, 122
ASN..................................................................................... 282
Blood alcohols............................................................... 95, 127
Carbon disulfide................................................................... 123
ASP............................................................................. 281, 282
Borneol................................................................................ 132
Carbon tetrachloride...............................85, 123, 128, 129, 135
Aspon.................................................................. 119, 121, 122
Bornyl acetate..................................................................... 132
Carbophenothion................................................................. 119
ASTM Method D 1945.................................................. 141, 142
Bromacil.............................................................. 119, 121, 122
Carboxin.............................................................. 119, 121, 122
ASTM Method D 2306.......................................................... 141
Bromoacetic acid................................................................. 120
Carene, 3-........................................................................... 132
ASTM Method D 2887............................................................ 71
Bromobenzene.................................................................... 123
Carvone............................................................................... 132
ASTM Method D 4815.......................................................... 141
390
Caryophyllene...................................................................... 132
Phenomenex
Chromatogram Index
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GC
GC
GC
Caryophyllene, -................................................................ 132
Cyclohexanone.................................................................... 141
Dibutylsulfide....................................................................... 143
Caryophyllene, -................................................................. 132
Cyclohexylamine.................................................................. 138
Dicamba.............................................................................. 120
Cell culture.......................................................................... 281
Cyclopentane....................................................................... 137
Dicamba (methyl ester)................................................ 121, 122
Chloramben......................................................................... 120
Cyclopentanol...................................................................... 136
Dichlofenthion..................................................... 119, 121, 122
Chloramben (methyl ester)........................................... 121, 122
Cyclopenta[c,d]pyrene........................................................... 84
Dichlone................................................................................ 92
Chlorcyclizine................................................................ 82, 127
2,4-D................................................................................... 120
Dichloroacetic acid.............................................................. 120
Chlordane, -.......................................................... 80, 87, 118
2,4-D (methyl ester)..................................................... 121, 122
Dichlorobenzene, 1,2-.................................. 115, 116, 123, 134
Chlordane (), cis-......................................................... 92, 118
DACP................................................................................... 133
Chlordane, -........................................................... 80, 87, 118
Dalapon............................................................................... 120
Chlordane (), trans-...................................................... 92, 118
2,4-DB................................................................................. 120
Dichlorobenzidine, 3,3'-....................................... 115, 116, 138
Chlorfenvinphos................................................................... 119
2,4-DB (methyl ester).................................................. 121, 122
Dichlorobenzoic acid, 3,5-................................................... 120
Chlorinated pesticides........................................... 80, 118, 120
DBCP..................................................................................... 92
Dichlorobenzoic acid (methyl ester), 3,5-..................... 121, 122
Chloroacetic acid................................................................. 120
DBOB................................................................................... 120
Dichloroethane, 1,1-.................................................... 123, 136
Chloroaniline, 4-.......................................... 115, 116, 136, 138
DCAA................................................................................... 120
Dichloroethane, 1,2-...............................85, 123, 128, 129, 135
Chlorobenzene................................98, 123, 128, 129, 134, 135
DCB....................................................................................... 81
Dichloroethene, 1,1-...............................85, 123, 128, 129, 135
Chlorobenzilate...................................................... 92, 115, 116
DCPA (Dacthal).............................................. 92, 120, 121, 122
Dichloroethene, 1,2-............................................................ 135
Chlorodibromoacetic acid.................................................... 120
DDD, 4,4'-......................................................... 80, 87, 92, 118
Dichloroethene, cis-1,2-...................................... 123, 128, 129
Chloroethane....................................................................... 123
DDE, 4,4'-.......................................................... 80, 87, 92, 118
Dichloroethene, trans-1,2-................................................... 123
Chloroform...................................123, 128, 129, 135, 136, 137
DDT, 4,4'-.......................................................... 80, 87, 92, 118
Dichloroethylene, 1,2-.......................................................... 136
Chloromethane.................................................................... 123
Decachlorobiphenyl............................................... 87, 118, 124
Dichloromethane................................................. 128, 129, 135
Chloro-3-methylphenol, 4-......................76, 115, 116, 124, 137
Decahectane....................................................................... 139
Dichlorophenol, 2,4-...............................76, 115, 116, 124, 137
Chloronaphthalene, 2-................................................. 115, 116
Decanal............................................................... 131, 132, 137
Dichlorophenol, 2,6-...............................76, 115, 116, 124, 137
Chloroneb.............................................................................. 92
Decane (C10)......71, 72, 73, 74, 79, 88, 98, 124, 131, 134, 139,
140, 143
Dichlorprop.......................................................................... 120
Chlorophenol, 2-.....................................76, 115, 116, 124, 137

Chlorophenol, 4-.............................................................. 72, 98
Chlorophenylphenylether, 4-........................................ 115, 116
Chloropropylate..................................................................... 92
Chlorothalonil........................................................................ 92
Chlorotoluene, 2-......................................................... 123, 134
Chlorotoluene, 4-......................................................... 123, 134
Chlorpheniramine.......................................................... 82, 127
Chlorpropham...................................................... 119, 121, 122
Chlorpyrifos methyl.............................................. 119, 121, 122
Cholesterol.......................................................................... 131
Chrysene..........................................69, 84, 115, 116, 117, 134
Cinnamyl acetate................................................................. 132
Citronellal............................................................................ 132
Clofenvinfos................................................................. 121, 122
Clonazepam......................................................................... 127
Copaene.............................................................................. 132
Coumaphos......................................................... 119, 121, 122
Crotoxyphos......................................................... 119, 121, 122
Crude oil.............................................................................. 139
Cubenene, -...................................................................... 132
Cubenene, -....................................................................... 132
Cumene............................................................... 135, 141, 142
Cyanuric acid....................................................................... 133
Cycloate.............................................................. 119, 121, 122
Cyclohexane.........................................128, 129, 135, 140, 141
Cyclohexanol....................................................................... 136
Phenomenex
Decanol, n-.......................................................................... 136

Decanone, 2-....................................................................... 137
Decylamine.......................................................................... 138
DEET........................................................................... 121, 122
Demeton.............................................................. 119, 121, 122
Desipramine........................................................................ 127
Desmethyltramadol, N-........................................................ 126
Desmethyltramadol, o-........................................................ 126
Diallate.......................................................................... 92, 116
Diazepam...................................................................... 82, 127
Diazinon (Dimpylate)............................................ 119, 121, 122
Dibenz[a,h]acridine........................................................ 84, 117
Dibenz[a,j]acridine......................................................... 84, 117
Dibenz[a,h]anthracene......................69, 84, 115, 116, 117, 134
Dibenzo[c,g]carbazole, 7H-............................................ 84, 117
Dibenzofuran....................................................... 115, 116, 136
Dibenzo[a,e]pyrene........................................................ 84, 117
Dibenzo[a,h]pyrene........................................................ 84, 117
Dibenzo[a,i]pyrene......................................................... 84, 117
Dibenzo[a,l]pyrene................................................................. 84
Dibenzylamine............................................................... 86, 138
Dibromoacetic acid.............................................................. 120
Dibromochloromethane....................................................... 123
Dibromo-3-chloropropane, 1,2-........................................... 123
Dibromomethane................................................................. 123
Dibutylamine....................................................................... 138
Dichloropropane, 1,3-.......................................................... 123

Dichloropropane, 2,2-.......................................................... 123
Dichloropropene, 1,1-.......................................................... 123
Dichloropropene, cis-1,3-.................................................... 123
Dichloropropene, trans-1,3-................................................. 123
Dichlorotrifluoroethane........................................................ 123
Dichlorvos........................................................... 119, 121, 122
Dicrotophos......................................................... 119, 121, 122
Dicyclohexylamine................................................... 72, 98, 138
Dieldrin.............................................................. 80, 87, 92, 118
Diesel fuel........................................................................... 124
Diethylamine....................................................................... 138
Diethylaniline, N,N-........................................................ 86, 138
Diethylbenzene, 1,2-............................................................ 141
Diethylbenzene, 1,3-............................................................ 141
Diethylbenzene, 1,4-............................................................ 141
Diethylene glycol......................................................... 128, 133
Diethyl ether........................................................................ 124
Diethyl phthalate............................................ 75, 115, 116, 133
Diethylsulfide....................................................................... 143
Diglycerides......................................................................... 143
Appendices
Codeine........................................................... 82, 94, 126, 127
Decanol, 1-............................................................................ 98
Dichloropropane, 1,2-.................................................... 98, 123
Cocaine......................................................................... 82, 127
Decanol....................................................................... 131, 136
Dichloroprop (methyl ester).......................................... 121, 122
CHROMATOGRAM INDEX
Chlorpyrifos......................................................... 119, 121, 122
Decanoic acid...................................................................... 131
Dihexylamine................................................................. 98, 138

Diisopropylamine................................................................. 138
Diisopropyl ether.................................................................. 123
Dimenhydrinate..................................................................... 82
Dimethoate.................................................. 116, 119, 121, 122
Dimethoxyethane, 1,2-................................ 128, 129, 135, 141
Dimethylaminoazobenzene.......................................... 115, 116
391
Appendices
CHROMATOGRAM INDEX
Chromatogram Index
GC
GC
GC
Dimethylaniline, N,N-..................................................... 86, 138
Drug screen................................................................... 82, 127
Ethylene dibromide.............................................................. 123
Dimethylaniline, 2,4-...................................................... 86, 138
Drugs of abuse...................................................................... 94
Ethylene glycol (1,2-Ethanediol)................................... 133, 135
Dimethylaniline, 2,6-.............................................................. 98
Durene................................................................................ 141
Ethyl ether........................................................................... 135
Dimethylbenzidine, 3,3'-.............................................. 115, 116
Eicosadienoic acid methyl ester, cis-11-14-......................... 130
Ethyl-t-butyl ether................................................................ 123
Dimethylbutylamine, N,N-.................................................... 138
Eicosane (C20)......................................................... 71, 74, 139
Ethylhexanoic acid, 2-...................................................... 72, 98
Dimethyldisulfide................................................................. 143
Eicosapentaenoic acid methyl ester,cis-5,8,11,14,17-......... 130
Ethylhexanol, 2-................................................................... 136
Dimethylformamide............................................................. 138
Eicosatrienoic acid methyl ester, cis-8,11,14-...................... 130
Ethyl-1-hexanol, 2-.............................................................. 136
Dimethylformamide, N,N-.................................................... 135
Eicosatrienoic acid methyl ester, cis-11,14,17-.................... 130
Ethylmethacrylate................................................................ 124
Dimethylhexane, 2,3-........................................................... 140
Eicosenoic acid methyl ester, cis-11-................................... 130
Ethylmethanesulfonate................................................ 115, 116
Dimethylpentane.................................................................. 140
Elaidic acid.................................................................... 90, 131
Ethyl parathion.................................................................... 116
Dimethylpentane, 2,2-......................................................... 140
Elaidic acid methyl ester...................................................... 130
Ethylphenol, 2-.............................................................. 76, 124
Endosulfan I....................................................... 80, 87, 92, 118
Ethylphenol, 3-.............................................................. 76, 124
Endosulfan II...................................................... 80, 87, 92, 118
Ethylphenol, 4-.............................................................. 76, 124
Dimethyl-2-neopentyl-1-pentene, 4,4-................................ 142
Endosulfan sulfate............................................. 80, 87, 92, 118
Ethyltoluene, m-.................................................................. 141
Dimethylphenol, 2,3-..................................................... 76, 124
Endrin................................................................ 80, 87, 92, 118
Ethyltoluene, p-................................................................... 141
Dimethylphenol, 2,4-........................76, 98, 115, 116, 124, 137
Endrin aldehyde................................................. 80, 87, 92, 118
Etridiazole.............................................................................. 92
Dimethylphenol, 2,5-..................................................... 76, 124
Endrin ketone.................................................... 80, 87, 92, 118
Eucalyptol............................................................................ 132
Dimethylphenol, 2,6-............................................... 76, 98, 124
EPA Method 507.................................................................. 119
Eugenol............................................................................... 132
Dimethylphenol, 3,4-..................................................... 76, 124
EPA Method 508.................................................................... 92
Famfur......................................................... 116, 119, 121, 122
Dimethylphenol, 3,5-..................................................... 76, 124
EPA Method 552.................................................................. 120
Farnasene............................................................................ 132
Dimethylphenylamine, ,-................................................. 115
EPA Method 606............................................................ 75, 133
Farnesol.............................................................................. 132
Dimethyl phthalate......................................... 75, 115, 116, 133
EPA Method 610............................................................ 69, 117
Farnesyl acetate.................................................................. 132
Dimethylpyrazine, 2,3-......................................................... 131
EPA Method 8015................................................................ 124
Fatty Acid Methyl Esters (FAMEs)......................................... 130
Dimethyl sulfoxide............................................................... 138
EPA Method 8081.................................................................. 87
Fenamiphos......................................................... 119, 121, 122
DIN Method .................................................................. 81, 124
EPA Method 8081A................................................ 80, 118, 120
Fenarimol............................................................ 119, 121, 122
Dinitrobenzene, 1,3-.................................................... 115, 116
EPA Method 8082................................................................ 124
Fenitrothion......................................................... 119, 121, 122
Dinitro-2-methylphenol, 4,6-......................... 76, 116, 124, 137
EPA Method 8100.......................................................... 69, 117
Fensulfothion....................................................... 119, 121, 122
Dinitrophenol, 2,4-..................................76, 115, 116, 124, 137
EPA Method 8141................................................................ 119
Fentanyl............................................................................... 127
Dinitrotoluene, 2,4-...................................................... 115, 116
EPA Method 8260................................................................ 123
Fenthion.............................................................. 119, 121, 122
Dinitrotoluene, 2,6-...................................................... 115, 116
EPA Method 8270........................................................ 115, 116
Flavors................................................................................ 131
Dinoseb..........................................76, 115, 116, 120, 124, 137
Ephedrine............................................................................ 125
Fluoranthene....................................69, 84, 115, 116, 117, 134
Dinoseb (methyl ester)................................................. 121, 122
EPN..................................................................... 119, 121, 122
Fluorene...................................................69, 84, 115, 116, 117
Diolein................................................................................. 143
EPTC................................................................... 119, 121, 122
Fluorobenzene..................................................................... 123
Diolein, 1,3-......................................................................... 143
Erucic acid..................................................................... 90, 131
Fluorobiphenyl, 2-................................................................ 116
Dioxane, 1,4-........................................124, 128, 129, 135, 141
Erucic acid methyl ester...................................................... 130
Fluorophenol, 2-.................................................................. 116
Dioxathion................................................................... 121, 122
Ester.................................................................................... 143
Fluridone............................................................................. 119
Dipentylamine...................................................................... 138
Ethane......................................................................... 141, 142
Fluroene.............................................................................. 134
Diphenamide....................................................... 119, 121, 122
Ethanol.......89, 91, 95, 123, 124, 127, 130, 131, 134, 135, 136,
137, 141, 142
Diphenhydramine................................................................ 127
Diphenylamine..................................................... 115, 116, 138
Diphenylenediamine, 1,4-.................................................... 116
Disulfoton.................................................... 116, 119, 121, 122
DMF.................................................................................... 135
DMSO.................................................................................. 135
Docosadienoic acid methyl ester, cis-13,16-........................ 130
Docosahexaenoic acid methyl ester, cis-4,7,10,13,16,19-... 130
Dodecanal........................................................................... 132
Dodecane (C12).................... 71, 74, 88, 98, 134, 139, 140, 143
Dodecanol........................................................................... 136
Dodecanone, 2-................................................................... 137
Dopentacontane (C52)................................................... 74, 139
Dotriacontane (C32)................................................. 71, 74, 139
Doxylamine.................................................................... 82, 127
392
Ether................................................................................... 135
Ether, bis(2-Chloroethyl)............................................... 115, 116
Ether, 2-Chloroethylvinyl...................................................... 123
Ether, bis(chloroisopropyl).................................................... 115
Ether, bis(2-Chloroisopropyl)................................................ 116
Ethion.................................................................. 119, 121, 122
Ethoprop.............................................................. 119, 121, 122
Ethyl acetate...................................89, 130, 131, 134, 135, 141
Ethylaniline, N-.............................................................. 86, 138
Ethylbenzene.......... 88, 123, 128, 129, 134, 135, 140, 141, 142
Ethylbutanol, 2-................................................................... 136
Ethyl-1-butanol, 2-.............................................................. 136
Ethyl butyrate...................................................................... 131
Ethyl decanoate................................................................... 131
Fonofos............................................................... 119, 121, 122

Formamide.......................................................................... 135
Formic acid ethyl ester (Ethyl formate)................................. 135
Galactose............................................................................ 132
GC test mix...................................................................... 72, 98
Geranial....................................................................... 131, 132
Gereniol............................................................................... 132
Gerenyl acetate................................................................... 132
Germacrene......................................................................... 132
GLN..................................................................................... 281
GLU............................................................................. 281, 282
Glucose............................................................................... 132
Glutethimide........................................................................ 127
GLY . ............................................................................ 281, 282
Glycerin............................................................... 128, 128, 143
Glycerol....................................................................... 133, 143
Phenomenex
Chromatogram Index

GC
GC
GC
Glycols................................................................................. 133
ILE .............................................................................. 281, 282
Merphos oxide (Tribufos)...................................... 119, 121, 122
Gondonic acid................................................................ 90, 131
Indeno[1,2,3-cd]pyrene....................69, 84, 115, 116, 117, 134
MET..................................................................................... 281
GPR..................................................................................... 281
Isoamyl alcohol.................................................................... 130
Methadone.......................................................................... 127
Grob test mix......................................................................... 98
Isoborneol............................................................................ 132
Methallyl alcohol.................................................................. 136
Haloacetic acids.................................................................. 120
Isobutane..................................................................... 141, 142
Methamphetamine......................................................... 94, 125
Hazardous substance........................................................... 136
Isobutanol...................................................... 89, 124, 130, 141
Methane.............................................................................. 142
Hectane (C100).............................................................. 74, 139
Isobutyl acetate................................................................... 135
Methane, bis(2-Chloroethoxy)...................................... 115, 116
Heptachlor......................................................... 80, 87, 92, 118
Isobutylamine...................................................................... 138
Heptachlor epoxide............................................ 80, 87, 92, 118
Isodrin......................................................................... 115, 116
Methanol....89, 91, 95, 124, 127, 128, 129, 130, 134, 135, 136,
141, 142
Heptacontane (C70)............................................................... 74
Isopentane................................................................... 142, 143
Heptadecane (C17)............................................ 71, 74, 98, 139
Isopentylamine.................................................................... 138
Heptadecenoic acid methyl ester, cis-10-............................ 130
Isophorone................................................................... 115, 116
Heptanal.............................................................................. 137
Isopropalin........................................................... 119, 121, 122
Heptane (C7)....... 71, 74, 88, 134, 135, 139, 140, 141, 142, 143
Isopropanol.............................................95, 127, 135, 136, 141
Heptanol.............................................................................. 136
Isopropylbenzene......................................................... 123, 134
Heptanol, n-......................................................................... 136
Isopropyl ether..................................................................... 141
Heptanol, 4-......................................................................... 136
Isopropyltoluene, 4-............................................................. 123
Heptanone, 2-...................................................................... 137
Isosafrole..................................................................... 115, 116
Heptanone, 3-...................................................................... 137
Kepone.................................................................. 92, 115, 116
Heptylamine........................................................................ 138
Ketones............................................................................... 137
Heroin............................................................................ 82, 127
Leptophos............................................................ 119, 121, 122
Hexachloro-1,3-butadiene................................................... 115
Lignoceric acid.................................................................... 131
Hexachlorocyclopentadiene.................................... 92, 115, 116
Limonene.................................................................... 131, 132
Hexachloroethane........................................................ 115, 116
Limonene oxide, cis-............................................................ 132
Hexachloropropene...................................................... 115, 116
Limonene oxide, trans-........................................................ 132
Hexacontane (C60)................................................................ 74
Linalool................................................................................ 132
Hexadecane (C16).............................................. 71, 74, 98, 139
Linoleic acid.................................................................. 90, 131
Hexadecanol........................................................................ 136
Linoleic acid methyl ester.................................................... 130
Hexanal............................................................................... 137
Linolelaidic acid............................................................. 90, 131
Hexane (C6)............................71, 128, 129, 135, 139, 141, 143
Linolelaidic acid methyl ester............................................... 130
Hexanediol, 1,6-.................................................................... 98
Linolenic acid................................................................ 90, 131
Hexanol............................................................................... 136
Linolenic acid methyl ester.................................................. 130
Hexanol, n-.......................................................................... 136
Linolenic acid methyl ester, -.............................................. 130
Hexanol, 3-.......................................................................... 136
Lorazepam.......................................................................... 127
Hexanone (MBK), 2-............................................. 123, 135, 137
LSD..................................................................................... 127
Hexanone, 3-....................................................................... 137
LYS . .................................................................................... 281
Hexatriacontane (C36)............................................. 71, 74, 139
Malathion............................................................ 119, 121, 122
Hexazinone.......................................................... 119, 121, 122
MAM, 6-...................................................................... 126, 127
Hexenol, (Z)-3-..................................................................... 131
MAM-d3, 6-......................................................................... 126
Hexobarbital.................................................................. 82, 127
Mannose............................................................................. 132
Hexylamine.......................................................................... 138
MCPA.................................................................................. 120
HIS . ........................................................................... 281, 282
MCPA (methyl ester).................................................... 121, 122
Humulene............................................................................ 132
MCPP.................................................................................. 120
Hydrocarbons........................................................................ 73
MCPP (methyl ester).................................................... 121, 122
Hydrocodone......................................................... 82, 126, 127
MDA.............................................................................. 94, 125
Hydromorphone................................................................... 126
MDEA............................................................................ 94, 125
Hydroxyalprazolam, -........................................................ 127
MDMA........................................................................... 94, 125
HYP..................................................................................... 281
Melamine............................................................................ 133
Ibogaine.............................................................................. 127
Meperidine.......................................................................... 126
Ibuprofen....................................................................... 82, 127
Meprobamate................................................................ 82, 127
Icosahectane (C120)............................................................ 139
Merphos.............................................................. 119, 121, 122
Phenomenex
Methoxy-2-methyl-2-propane (MTBE), 2-............................ 135

Methylacetamide................................................................. 135
Methylaniline, N-........................................................... 86, 138
Methyl anisole, p-................................................................ 132
Methyl arachidate........................................................ 130, 131
Methyl behenate.......................................................... 130, 131
Methyl benzoate.................................................................. 132
Methylbutanal, 2-................................................................ 137
Methylbutane, 2-................................................................. 142
Methylbutanol, 2-.......................................................... 89, 130
Methylbutanol, 3-.......................................................... 89, 135
Methyl-2-butanone, 3-......................................................... 137
Methyl-2-butene, 2-............................................................ 142
Methyl-t-Butyl Ether (MTBE)................................ 123, 141, 142
Methylbutylketone....................................................... 128, 129
Methyl butyrate................................................................... 130
Methyl caprylate.................................................................... 98
Methylcholanthrene, 3-.................................. 84, 115, 116, 117
Methylchrysene, 5-................................................................ 84
Methylcyclohexane.............................................. 128, 129, 135
Methylcyclohexanol, 2-........................................................ 136
Methylcyclohexanol, trans-3-............................................... 136


Methylcyclohexanol, cis-2-.................................................. 136
Methylcyclopentane..................................................... 140, 141

Methyl decanoate.......................................................... 98, 130
Methyl-4,6-dinitrophenol, 2-................................................ 115
Methyl dodecanoate.............................................................. 98
Methyl elaidate.................................................................... 131
Appendices
Lidocaine............................................................................. 127
Methoxy ethanol, 2-............................................................. 135
LEU.............................................................................. 281, 282
Methoxychlor..................................................... 80, 87, 92, 118
CHROMATOGRAM INDEX
Hexachlorobenzene............................................... 92, 115, 116

Hexachlorobutadiene................................................... 116, 123
Methapyrilene................................................ 82, 115, 116, 127
Methylene chloride...................................... 123, 134, 135, 141

Methyl erucate..................................................................... 131
Methyl ethyl ketone............................................. 134, 135, 141
Methyl formate............................................................ 134, 141
Methyl gondonate................................................................ 131
Methyl heneicosanoate........................................................ 130
Methyl heptadecanoate........................................................ 130
393
Appendices
CHROMATOGRAM INDEX
Chromatogram Index
394
GC
GC
GC
Methylhexane, 2-................................................................. 140
Monocrotophos.................................................... 119, 121, 122
Norcodeine.......................................................................... 126
Methylhexane, 3-................................................................. 140
Monoglycerides................................................................... 143
Nordiazepam....................................................................... 127
Methyl hexanoate................................................................ 130
Monoolein............................................................................ 143
Norephedrine....................................................................... 125
Methyl-2-hexanone, 5-........................................................ 137
Monooleoyl-rac-glycerol, 1-................................................. 143
Norflurazon.......................................................... 119, 121, 122
Methyl-3-hexanone, 2-........................................................ 137
Morphine......................................................... 82, 94, 126, 127
Normeperidine..................................................................... 126
Methyl iodide....................................................................... 123
MTBE................................................................................... 135
Norpseudoephedrine............................................................ 125
Methylisobutylketone (MIBK)........................................ 123, 135
Myrcene, -......................................................................... 132
Norvaline..................................................................... 281, 282
Methyl laurate..................................................................... 130
Myristic acid.................................................................. 90, 131
Octacontane (C80)................................................................. 74
Methyl lignocerate............................................................... 130
Myristoleic acid............................................................. 90, 131
Octacosane (C28).................................................... 71, 74, 139
Methyl linelaidate................................................................ 131
Myristoleic acid methyl ester............................................... 130
Octadecane (C18).............................................. 71, 74, 98, 139
Methyl linoleate................................................................... 131
NALA................................................................................... 281
Octanal........................................................................ 132, 137
Methyl linolenate................................................................. 131
Naled................................................................... 119, 121, 122
Octane (C8).................................................71, 74, 98, 139, 143
Methylmethacrylate............................................................. 124
Naphthalenamine, 1-........................................................... 115
Octanediol, 1,8-..................................................................... 98
Methylmethanesulfonate.............................................. 115, 116
Naphthalenamine, 2-........................................................... 115
Octanol.......................................................................... 98, 136
Methyl myristate.......................................................... 130, 131
Naphthalene................. 69, 72, 84, 98, 115, 116, 117, 123, 134
Octanone, 2-.................................................................. 98, 137
Methyl myristoleate............................................................. 131
Naphthoquinone, 1,4-.................................................. 115, 116
Octylamine.......................................................................... 138
Methylnaphthalene, 1-........................................... 98, 115, 116
Naphthylamine, 1-............................................................... 116
Oleic acid...................................................................... 90, 131
Methylnaphthalene, 2-......................69, 84, 115, 116, 117, 136
Naphthylamine, 2-............................................................... 116
Oleic acid methyl ester........................................................ 130
Methyl-5-nitroaniline, 2-.............................................. 115, 116
Napropamide....................................................... 119, 121, 122
Opiates.......................................................................... 94, 126
Methyl-N-nitrosoethanamine, N-.......................................... 116
Natural gas.......................................................................... 142
Orange oil............................................................................ 132
Methyl octanoate................................................................. 130
Neral........................................................................... 131, 132
Organoamines..................................................................... 138
Methyl oleate....................................................................... 131
Nervonic acid methyl ester.................................................. 130
Organochlorine pesticides.............................................. 87, 118
Methyl palmitate.......................................................... 130, 131
Neryl acetate....................................................................... 131
Organophosphorous pesticides............................................ 119
Methyl palmitelaidate.......................................................... 131
Nicotine......................................................................... 82, 127
ORN............................................................................. 281, 282
Methyl palmitoleate............................................................. 131
Nitroaniline, 2-............................................. 115, 116, 136, 138
Oxadiazon............................................................ 119, 121, 122
Methyl parathion.......................................... 116, 119, 121, 122
Nitroaniline, 3-............................................. 115, 116, 136, 138
Oxazepam........................................................................... 127
Methyl pentadecanoate........................................................ 130
Nitroaniline, 4-............................................. 115, 116, 136, 138
Oxycodone........................................................................... 126
Methylpentanal, 2-............................................................... 137
Nitrobenzene....................................................... 115, 116, 138
Oxyfluoroen......................................................... 119, 121, 122
Methylpentane, 2-............................................................... 143
Nitrofen................................................................................. 92
Oxymorphone........................................................ 82, 126, 127
Methyl-2-pentanone, 3-....................................................... 137
Nitrogen compounds............................................................ 138
Palmitelaidic acid........................................................... 90, 131
Methyl-2-pentanone (MIBK), 4-.................................... 124, 137
Nitromethane....................................................... 128, 129, 135
Palmitic acid.................................................................. 90, 131
Methylphenol, 2-.............................76, 115, 116, 124, 136, 137
Nitrophenol, 2-........................................76, 115, 116, 124, 137
Palmitoleic acid............................................................. 90, 131
Methylphenol, 3-.....................................76, 115, 116, 124, 137
Nitrophenol, 4-................................76, 115, 116, 120, 124, 137
Palmitoleic acid methyl ester............................................... 130
Methylphenol, 4-.............................76, 115, 116, 124, 136, 137
Nitrophenol (methyl ester), 4-...................................... 121, 122
Paraldehyde......................................................................... 137
Methylpiperidine, 2-....................................................... 86, 138
Nitropropane........................................................................ 138
Parathion............................................................. 119, 121, 122
Methylpropanol, 2-...................................................... 135, 136
Nitroquinoline-n-oxide, 4-............................................ 115, 116
Paraffin wax.......................................................................... 79
Methyl-1-propanol, 2-......................................................... 136
Nitrosodibutylamine, N-............................................... 115, 116
Pebulate (Tillam).................................................. 119, 121, 122
Methyl-2-propanol, 2-................................................. 134, 141
Nitrosodiethylamine, N-............................................... 115, 116
Pendimethalin...................................................... 119, 121, 122
Methyl-2-Propenal, 2-......................................................... 137
Nitrosodimethylamine, N-.................................... 115, 116, 138
Pentachlorobenzene.................................................... 115, 116
Methylpyridine, 2-................................................................ 116
Nitrosomethylethylamine, N-................................................ 115
Pentachloronitrobenzene....................................... 87, 115, 116
Methylpyrrolidine, 1-............................................................ 138
Nitrosomorpholine, N-.................................................. 115, 116
Pentachlorophenol..........................76, 115, 116, 120, 124, 137
Methylpyrrolidinone, n-........................................................ 135
Nitrosopiperidine, N-.................................................... 115, 116
Pentachlorophenol (methyl ester)................................. 121, 122
Methyl stearate............................................................ 130, 131
Nitroso-di-n-propylamine, N-............................... 115, 116, 138
Pentacontane (C50)....................................................... 74, 139
Methyl tricosanoate............................................................. 130
Nitrosopyrrolidine, N-................................................... 115, 116
Pentadecane (C15)................................71, 72, 74, 98, 139, 143
Methyl tridecanoate............................................................. 130
Nonachlor, trans-................................................................... 92
Pentadecanol....................................................................... 136
Methyl undecanoate...................................................... 98, 130
Nonacontane (C90)................................................................ 74
Pentadecenoic acid methyl ester, cis-10-............................. 130
Metolachlor......................................................... 119, 121, 122
Nonadecane.......................................................................... 98
Pentamethyl-3-heptene (cis, trans), 2,2',4,6,6'-................... 142
Metribuzin........................................................... 119, 121, 122
Nonanal................................................................. 98, 132, 137
Pentanal.............................................................................. 137
Mevinphos........................................................... 119, 121, 122
Nonane (C9)................................................71, 74, 98, 139, 141
Pentane (C5)............. 71, 88, 134, 135, 139, 140, 141, 142, 143
MGK.................................................................... 119, 121, 122
Nonanoic acid...................................................................... 131
Pentanol isomers................................................................. 136
Mineral oil............................................................................. 79
Nonanone, 2-....................................................................... 137
Pentanol, n-......................................................................... 135
Molinate.............................................................. 119, 121, 122
Nonhalogenated organics.................................................... 124
Pentanol, tert-...................................................................... 141
Monobromoacetic acid........................................................ 120
Nonylamine......................................................................... 138
Pentanone, 2-...................................................................... 137
Monochloroacetic acid......................................................... 120
Nootkatone.......................................................................... 132
Pentanone, 3-...................................................................... 137
Phenomenex
Chromatogram Index

GC
GC
Pentene, cis-2-.................................................................... 142
Propanediol, 1,3-................................................................. 133
Terbacil................................................................ 119, 121, 122
Pentene, trans-2-................................................................. 142
Propanenitrile...................................................................... 124
Terbufos.(Terbuphos)........................................... 119, 121, 122
Pentobarbital......................................................... 82, 126, 127
Propanol................................................................ 89, 136, 141
Terbuthylazine............................................................. 121, 122
Pentylamine......................................................................... 138
Propanol, n-............................................95, 127, 134, 135, 136
Terbutryn............................................................. 119, 121, 122
Permethrin............................................................................. 92
Propanol, 1-................................................................. 130, 141
Terphenyl, p-....................................................................... 116
Perylene.............................................................................. 116
Propazine............................................................ 119, 121, 122
Terpinene, -....................................................................... 132
Pesticides...................................................... 92, 119, 121, 122
Propenal.............................................................................. 137
Terpineol.............................................................................. 132
Petroleum oxygenates......................................................... 141
Propoxyphene...................................................................... 127
Terpineol, 4-........................................................................ 132
PHE............................................................................. 281, 282
Propyl acetate...................................................................... 135
Terpineol, -................................................................ 131, 132
Phellandrene, -.................................................................. 132
Propylbenzene..................................................... 141, 142, 143
Terpinolene.......................................................................... 132
Phenacetin.................................................... 82, 115, 116, 127
Propylbenzene, n-........................................................ 123, 134
Tetrachlorobenzene, 1,2,4,5-....................................... 115, 116
Phenanthrene...................................69, 84, 115, 116, 117, 134
Propylene glycol.................................................................. 131
Tetrachloroethane, 1,1,1,2-.................................................. 123
Phencyclidine.............................................................. 125, 127
Pseudocumene.................................................................... 141
Tetrachloroethane, 1,1,2,2-.................................................. 123
Phenobarbital........................................................ 82, 126, 127
Pseudoephedrine................................................................. 125
Tetrachloroethene................................................................ 123
Phenol....................................................76, 115, 116, 124, 137
Pyrene..............................................69, 84, 115, 116, 117, 134
Tetrachloroethylene....................................................... 98, 135
Phenols................................................................. 76, 124, 137
Pyridine........................................115, 116, 128, 129, 135, 138
Tetrachlorophenol................................................................ 116
Phentermine................................................................ 116, 125
Residual solvents................................................... 85, 128, 129
Tetrachlorophenol, 2,3,4,6-............................ 76, 115, 124, 137
Phenylacetate...................................................................... 141
Ribose (isomers), D-............................................................ 132
Tetrachlorovinphos (Stirofos)................................................ 119
Phenylacetylene.................................................................. 141
Ronnel................................................................. 119, 121, 122
Tetrachloro-m-xylene..................................................... 87, 124
Phenylenediamine, 1,4-....................................................... 115
Rosemary oil........................................................................ 132
Tetracontane (C40)...............................71, 73, 74, 79, 124, 139
Phenylethanol...................................................................... 136
Safrole......................................................................... 115, 116
Tetracosane (C24).................................................... 71, 74, 139
Phenylethanol, 2-................................................................. 136
Secbumeton................................................................ 121, 122
Tetradecane (C14)...............................71, 74, 98, 131, 139, 143
Phenylethylamine, -.................................................... 86, 138
Secobarbital.......................................................... 82, 126, 127
Tetradecanol........................................................................ 136
Phenylethylamine, -..................................................... 86, 138
Semivolatile organics................................................... 115, 116
Tetraethyl pyrophosphate (methyl ester)....................... 121, 122
Phenylketonuria................................................................... 281
SER............................................................................. 281, 282
Tetrahydrofuran (THF).................................. 123, 128, 129, 135
Phenylmethanol................................................................... 136
Silvex.................................................................................. 120
Tetralin................................................................ 128, 129, 135
Phenyl-1-propanol, 3-.......................................................... 136
Silvex (methyl ester).................................................... 121, 122
Tetratetracontane (C44)........................................... 71, 74, 139
Phorate........................................................ 116, 119, 121, 122
Simazine..................................................................... 121, 122
TFMPP................................................................................. 125
Phosmet.............................................................. 119, 121, 122
Simetryn.............................................................. 119, 121, 122
THC..................................................................................... 125
Phosphamidon..................................................... 119, 121, 122
Simulated distillation....................................... 74, 98, 139, 143
THC, Carboxy-..................................................................... 125
Phthalate esters............................................................. 75, 133
Simulated distillation markers................................................ 71
Theanine............................................................................. 282
Phthalate, bis(2-Ethylhexyl)............................ 75, 115, 116, 133
Sitosterol, -........................................................................ 131
Thionazin............................................................. 119, 121, 122
Phthalate, di-n-butyl...................................... 75, 115, 116, 133
Solvent (carbon disulfide)..................................................... 138
Thiophene........................................................................... 143
Phthalate, di-n-octyl...................................... 75, 115, 116, 133
Solvent (methylene chloride).......................................... 88, 134
Thiophene, 2-methyl............................................................ 143
Picloram.............................................................................. 120
Solvents...................................................................... 135, 141
Thiophene, 3-methyl............................................................ 143
Picloram (methyl ester)................................................ 121, 122
Solvents, industrial.............................................................. 135
Thiophenes, C2-.................................................................. 143
Picoline, 2-.......................................................................... 115
Squalene............................................................................. 131
THR..................................................................................... 281
Picoline, 3-.......................................................................... 135
Stearic acid................................................................... 90, 131
Thujene, -......................................................................... 132
Pinene, -........................................................................... 132
Sterols................................................................................. 131
Tocomonoenol, -................................................................ 131
Pinene, -............................................................................ 132
Stigmasta-3,5-diene............................................................ 131
Tocomonoenol, -................................................................ 131
Piperidine...................................................................... 86, 138
Stigmasta-3,5,22-triene...................................................... 131
Tocopherol, -..................................................................... 131
PolyBrominated Diphenyl Ethers (PBDEs)............................. 117
Stigmasterol........................................................................ 131
Tocopherol, -..................................................................... 131
Polynuclear Aromatic Hydrocarbons (PAHs)...... 69, 84, 117, 134
Stirofos................................................................ 119, 121, 122

Styrene........................................................ 123, 135, 141, 142
Tocopherol, -...................................................................... 131
PONA................................................................................... 140
PRO............................................................................. 281, 282
Styrene impurities................................................................ 134
Toluene........... 88, 123, 128, 129, 134, 135, 140, 141, 142, 143
Procaine........................................................................ 82, 127
Sugars (TMS)....................................................................... 132
Appendices
Toluidine, 2-......................................................................... 115
Profluralin............................................................ 119, 121, 122
Sulfotep............................................................... 119, 121, 122
Toluidine, m-.................................................................. 86, 138
Prometon............................................................. 119, 121, 122
Sulfur compounds............................................................... 143
Toluidine, o-........................................................... 86, 116, 138
Prometryn............................................................ 119, 121, 122
2,4,5-T................................................................................ 120
Tramadol............................................................................. 126
Pronamide (Propyzamide)............................ 116, 119, 121, 122
2,4,5-T methyl ester.................................................... 121, 122
Triademifon......................................................................... 119
Propachlor..................................................... 92, 119, 121, 122
TCMX............................................................................ 81, 118
Triadimeton................................................................. 121, 122
Propanal.............................................................................. 137
Tebuthiuron......................................................... 119, 121, 122
Triazolam............................................................................. 127
Propane....................................................................... 141, 142
Temazepam......................................................................... 127
Tribromoacetic acid............................................................. 120
Propanediol, 1,2-................................................................. 133
TEPP.................................................................................... 119
Tribromophenol, 2,4,6-........................................................ 116
Phenomenex
Tokuthion............................................................. 119, 121, 122
CHROMATOGRAM INDEX
GC
395
Chromatogram Index
GC
GC
Tributylamine....................................................................... 138
Xylene, m-.............. 88, 128, 129, 134, 135, 140, 141, 142, 143
Tricarpin.............................................................................. 143
Xylene, m,p-........................................................................ 123
Trichlorfon........................................................... 119, 121, 122
Xylene, o-....... 88, 123, 128, 129, 134, 135, 140, 141, 142, 143
Trichloroacetic acid.............................................................. 120
Xylene, p-............... 88, 128, 129, 134, 135, 140, 141, 142, 143
Trichlorobenzene, 1,2,3-...................................................... 123
Xylose.................................................................................. 132
Trichlorobenzene, 1,2,4-...................................... 115, 116, 123
Yeates crude oil................................................................... 139
Trichloroethane, 1,1,1-............................85, 123, 128, 129, 135
Ylang Ylang oil..................................................................... 132
Trichloroethane, 1,1,2-......................................................... 123
Zinophos (Thionazin)............................................................ 116
Trichloroethene.................................................................... 123
Trichloroethylene................................................. 128, 129, 135
Trichlorofluoromethane........................................................ 123
Trichloronate........................................................ 119, 121, 122
Trichlorophenol, 2,4,5-....................76, 115, 116, 124, 136, 137
Trichlorophenol, 2,4,6-............................76, 115, 116, 124, 137
Trichloropropane, 1,2,3-.............................................. 120, 123
Trichlorotrifluoroethane........................................................ 123
Tricyclazole.......................................................... 119, 121, 122
Tricyclene............................................................................ 132
Tridecane......................................................... 72, 98, 141, 143
Tridecanol............................................................................ 136
Tridecanone, 2-.................................................................... 137
Triethylamine....................................................................... 138
Triethylene glycol................................................................. 133
Triethylphosphorothioate...................................................... 116
Trifluralin............................................................. 119, 121, 122
Triglycerides.................................................................. 79, 143
Trimethylbenzene, 1,2,4-..................................................... 123
Trimethylbenzene, 1,3,5-..................................................... 123
Trimethylpentane, 2,2,3-...................................................... 140
Trimethyl-1-pentene, 2,4,4-................................................. 142
Appendices
| CHROMATOGRAM INDEX
Trimipramine....................................................................... 127
Trinitrobenzene, 1,3,5-................................................. 115, 116
Triolein................................................................................ 143
Trithion.................................................................................. 92
TRP............................................................................. 281, 282
TYR............................................................................. 281, 282
Undecane (C11)..........................................71, 74, 98, 139, 141
Undecanol, 1-.......................................................... 72, 98, 136
Undecanone, 2-................................................................... 137
Unsaturated Fatty Acid Methyl Esters (FAMEs)................ 90, 131
USP Method 467.................................................... 85, 128, 129
VAL . ............................................................................ 281, 282
Valencene............................................................................ 132
Valeric acid.......................................................................... 131
Vanillin................................................................................. 131
Vernolate............................................................. 119, 121, 122
Vinyl acetate........................................................................ 123
Vinyl chloride....................................................................... 123
Volatile organics.................................................................. 123
Water............................................................................. 89, 135
Whiskey............................................................................... 130
396
Phenomenex
Chromatogram Index

HPLC
HPLC
HPLC
Acebutolol................................................................... 185, 269

Acenaphthene..................................................... 166, 188, 365
Acenaphthylene................................................... 166, 188, 196
Acetaminophen............................................ 185, 196, 200, 269
Acetate................................................................................ 244
Acetic acid............................................229, 230, 231, 255, 366
Acetone............................................................................... 183
Acetophenone....... 182, 183, 184, 226, 239, 267, 268, 276, 365
Acetylalanine, N- (chiral)...................................................... 315
Acetylene carboxylic acid..................................................... 232
Acetylgalactosamine, N-...................................................... 232
Acetylglucoseamine, N-....................................................... 232
Acetylleucine, N- (chiral)...................................................... 315
Acetylmethionine, N- (chiral)................................................ 315
Acetylvaline, N- (chiral)........................................................ 315
Acyclovir.............................................................................. 201
Adenine....................................................................... 196, 253
Adenosine............................................................................ 196
ADP..................................................................................... 196
ADP, 5'-............................................................................... 164
Alanine, D/L- (chiral)............................................................ 315
Alanine, Z- (chiral)............................................................... 315
Alanylglycine (chiral)............................................................ 315
Alanylglycyl-glycine (chiral).................................................. 315
Aldicarb............................................................................... 188
Aldicarb sulfone................................................................... 188
Aldicarb sulfone-related impurity......................................... 188
Aldicarb sulfoxide................................................................ 188
Aliphatic acids..................................................................... 230
Alkaline phosphatase........................................................... 298
Alloisoleucine, D/L- (chiral).................................................. 315
Allothreonine, D/L- (chiral)................................................... 315
Alprenolol............................................................ 170, 213, 250
Amino sugars...................................................................... 232
Aminobenzoic acid, p-......................................................... 251
Amino-n-butyric acid (chiral)................................................ 315
Amitriptyline.........................................172, 185, 198, 337, 339
Amoxapine.......................................................................... 185
AMP.................................................................................... 196
AMP, 5'-............................................................................... 164
Amylase, -......................................................... 292, 293, 294
Anesthetics, local................................................................. 174
Anions................................................................. 244, 248, 365
Anthracene.................................................. 166, 188, 196, 365
Antibacterials....................................................................... 197
Antihistamines......................................170, 212, 251, 252, 254
Anti-viral medicines............................................................. 201
Apomyoglobin digest........................................................... 286
Aprotinin.............................................................................. 298
Arabinose............................................................................ 232
Arginine, D/L- (chiral)........................................................... 315
ARG-Sub P........................................................................... 200
Aromatic acids..................................................................... 195
Aromatics............................................................................ 226
Ascorbic acid....................................................................... 201
Asparagine (chiral)............................................................... 315
Asparagine, Z- (chiral).......................................................... 315
Aspartic acid, D-.................................................................. 366
Aspartic acid, D/L- (chiral)................................................... 315
Aspartic acid, L-.................................................................. 366
Atenolol............................................................... 170, 189, 213

ATP...................................................................................... 196
ATP, 5'-................................................................................ 164
Atropine (chiral)................................................................... 313
Azo dyes.............................................................................. 188
Baclofen (chiral)................................................................... 315
Barban................................................................................. 166
Basic compounds........................................ 171, 252, 257, 339
Baygon (Propoxur)............................................................... 188
Bendroflumethiazide............................................................ 330
Benzaldehyde...................................................................... 196
Benzamide.......................................................................... 365
Benz[a]anthracene............................................... 166, 188, 196
Benzene...............................................182, 226, 241, 267, 268
Benzodiazepines.......................................................... 174, 187
Benzo[b]fluoranthene........................................... 166, 188, 196
Benzo[k]fluoranthene........................................... 166, 188, 196
Benzoic acid................................................................ 195, 196
Benzo[g,h,i]perylene............................................ 166, 188, 196
Benzophenone..................................................................... 365
Benzo[a]pyrene.................................................... 166, 188, 196
Benzo[e]pyrene............................................................ 166, 196
Benzoylglutamic acid, N- (chiral).......................................... 315
Benzoylleucine, N- (chiral)................................................... 315
Benzoylphenylalanine, N- (chiral)......................................... 315
Benzoylphenylglycine, N- (chiral)......................................... 315
Benzoylvaline, N- (chiral)..................................................... 315
Benzyl alcohol..................................................................... 196
Beta-blockers...................................................................... 170
Bifonazole............................................................................ 330
Bioethanol........................................................................... 229
Biphenyl...................................................................... 218, 365
Bisoprolol............................................................................ 170
BOC-phenylalanine, D/L- (chiral).......................................... 315
Boronophenylalanine, (chiral)............................................... 315
Bovine Cytochrome c........................................................... 297
Bovine insulin...................................................................... 299
Bovine thyroglobulin............................................................ 365
Bromate.............................................................................. 248
Bromazepam....................................................................... 236
Bromide............................................................... 244, 248, 365
Brompheniramine........................................................ 170, 212
BSA..................................................................... 292, 293, 298
BSA tryptic digest........................................................ 285, 288
Butanone, 2-........................................................................ 183
Butylbenzyl phthalate........................................................... 198
Butyrophenone.................................................................... 183
Caffeine....................................................................... 174, 196
Canine Cytochrome c........................................................... 297
Capric acid.......................................................................... 196
Carbadox............................................................................. 197
Carbamate pesticides.......................................................... 188
Carbamates......................................................................... 166
Carbaryl....................................................................... 166, 188
Carbofuran.................................................................. 166, 188
Carbofuran, 3-hydroxyl-....................................................... 188
Carbohydrates..................................................... 231, 232, 366
Carboxylic acids................................................................... 232
Casein, -, tryptic digest...................................................... 273
Catechin.............................................................................. 204
Catechin gallate................................................................... 204

Catecholamines................................................................... 255
CDP, 5'-............................................................................... 164
Cellobiose............................................................................ 232
Cereal.................................................................................. 213
Chiral LC test mix................................................................ 366
Chlorate............................................................................... 248
Chlordiazepoxide................................................................. 174
Chloride............................................................... 244, 248, 365
Chlorobenzoic acid, 4-......................................................... 185
Chlorocinnamic acid, 4-....................................................... 185
Chloropyramine........................................................... 170, 212
Chlorpheniramine.................170, 185, 200, 212, 252, 257, 269
Chlorpropham...................................................................... 166
Chrysene............................................................. 166, 188, 196
Appendices
Chymotrypsin tryptic digest, -........................................... 297

Cimetidine........................................................................... 269
Citric acid.................................................... 230, 231, 255, 366
Clomipramine.............................................................. 172, 185
Clonazepam......................................................... 174, 187, 236
CMP, 5'-............................................................................... 164
Codeine............................................................................... 257
Cold medicine...................................................................... 200
Corn oil................................................................................ 167
Coronene............................................................................. 218
Corticosterone..................................................................... 196
Cortisone..................................................... 181, 187, 198, 199
Cortisone acetate......................................... 181, 187, 196, 199
Cotinine............................................................................... 202
CTP, 5'-................................................................................ 164
Cyanocobalamin.................................................................. 298
Cyanuric acid....................................................................... 188
Cyclic polymer..................................................................... 220
Cystine (chiral)..................................................................... 315
Cytochrome c, tryptic digest of............................................ 200
Cytosine...................................................................... 365, 366
Dansyl-D-valine (cyclohexylammonium salt), N-................... 366
Dansyl-L-valine (cyclohexylammonium salt), N-................... 366
Dansylnorvaline, N- (chiral).................................................. 315
Dansylphenylalanine, N- (chiral)........................................... 315
Dansylthreonine, N- (chiral).................................................. 315
Dansyltryptophan, N- (chiral)............................................... 315
Decanophenone................................................................... 183
Deoxycorticosterone acetate................................................ 187
Desipramine........................................................ 185, 198, 257
Desmethyldoxepin............................................................... 185
Dextran................................................................................ 225
Dextromethorphan............................................................... 200
Diaminopimelic acid, 2,6- (chiral)......................................... 315
Diazepam.............................................174, 185, 187, 236, 257
Dibenzo[a,h]anthracene....................................... 166, 188, 196
Dibromophenol, 2,4-............................................................ 203
Dibutyl phthalate................................................................. 198
Dichloronitroaniline.............................................................. 178
Dichlorophenol, 2,5-............................................................ 203
Dichlorophenol, 2,6-............................................................ 203
Dichlorophenol, 3,4-............................................................ 203
Dichlorophenol, 3,5-............................................................ 203
CHROMATOGRAM INDEX |
Phenomenex
Chymotrypsin, -................................................................ 297
397
Chromatogram Index
HPLC
HPLC
HPLC
Dietary fiber......................................................................... 243

Diethyl phthalate.................................................................. 198
Diethylhexyl phthalate.................................................. 167, 218
Diflunisal............................................................................. 185
Digitoxin.............................................................................. 189
Digoxin................................................................................ 189
Dihydrokawain..................................................................... 194
Dihydromethysticin.............................................................. 194
Diltiazem............................................................................. 269
Dimethylaniline.................................................................... 178
Dimethylphenol, 2,3-........................................................... 203
Dimethylphenol, 2,5-........................................................... 203
Dimethylphenol, 2,6-........................................................... 203
Dimethylphenol, 3,4-........................................................... 203
Dimethylphenol, 3,5-........................................................... 203
Dimethyl phthalate............................................................... 198
Dimethyl yellow................................................................... 188
Dinitrobenzene, 1,3-............................................................ 187
Ethyl-2-methyl-3-indoleacetate........................................... 332
Ethyl paraben.............................................................. 196, 250
Ethynyl estradiol (chiral)....................................................... 324
Explosives........................................................................... 187
Farnesene............................................................................ 219
Fast Garnet GBC.................................................................. 188
Fenoprofen.......................................................................... 187
Fenuron............................................................................... 166
Fermentation broths............................................................ 232
Flavanoids........................................................................... 174
Flourene.............................................................................. 166
Fluometuron........................................................................ 166
Fluoranthene....................................................... 166, 188, 196
Fluorene.............................................................. 166, 188, 196
Fluoride............................................................... 244, 248, 365
Flurazepam.......................................................................... 174
FMOC-Leucine, N- (chiral).................................................... 315
FMOC-Phenylalanine, N- (chiral).......................................... 315
FMOC-Valine, N- (chiral)...................................................... 315
Folic acid............................................................................. 201
Food softeners..................................................................... 232
Formate............................................................................... 244
Formic acid.................................................. 230, 231, 255, 366
Formylmethionine, N- (chiral)............................................... 315
Formylvaline, N- (chiral)....................................................... 315
Fructofuranosylnystose, 1F-................................................. 164
Fructo-oligosaccharides....................................................... 164
Fructose...............................................199, 230, 231, 232, 366
Fumaric acid........................................................................ 232
Furazolidone........................................................................ 197
Galactose............................................................ 230, 231, 232
Galactosidase, -................................................................. 298
Gallic acid............................................................................ 204
Gallocatechin gallate........................................................... 204
Ganciclovir........................................................................... 201
GC test mix.......................................................................... 226
GDP, 5'-............................................................................... 164
GFC calibration curve........................................................... 243
Glucocorticoids.................................................................... 198
Glucose........................ 164, 199, 229, 230, 231, 232, 243, 366
Glutamic acid, D/L- (chiral).................................................. 315
Glutamic dehydrogenase..................................................... 298
Glutamine, D/L- (chiral)........................................................ 315
Glycerol............................................................... 229, 232, 243
Glycolic acid........................................................................ 255
HSA..................................................................................... 294
Hu IgA 300KDa.................................................................... 293
Human epidermal growth factor............273, 286, 286, 290, 290
Human gamma globulin....................................................... 365
Human Ig-G......................................................................... 288
Human IgG digest................................................................ 288
Human insulin...................................................................... 299
Human serum.............................................................. 292, 294
Hyaluronic acid.................................................................... 243
Hydrocarbons...................................................................... 218
Hydrocortisone............................................ 187, 196, 198, 332
Hydrocortisone acetate........................................................ 198
Appendices
CHROMATOGRAM INDEX
Dinitrobenzoyl)--methylbenzylamine, (R)-(-)-N-(3,5-.......... 366
398
Dinitrobenzoyl)--methylbenzylamine, (S)-(+)-N-(3,5-......... 366

Dinitrotoluene, 2,4-.............................................................. 187
Dioctyl phthalate.................................................................. 198
Diphenhydramine.........................170, 212, 252, 338, 339, 345
Disopyramide (chiral)........................................................... 321
Diuron................................................................................. 166
DNA..............................................275, 305, 306, 307, 308, 309
DNA, Crude.................................................................. 275, 307
DNA, FAM-labeled................................................................ 306
DNA, purified mers.............................................................. 305
DOPA [3-(3,4-dihydroxyphenyl)-alanine], D/L- (chiral).......... 315
DOPA, L-.............................................................................. 255
Dopamine............................................................................ 255
Doxepin....................................................................... 185, 198
EPA Method 300.................................................................. 248
EPA Method 531.................................................................. 188
EPA Method 610.......................................................... 166, 188
EPA Method 632.................................................................. 166
EPA Method 8310................................................................ 196
Epicatechin.......................................................................... 204
Epicatechin gallate.............................................................. 204
Epigallo catechin................................................................. 204
Epigallocatechin gallate....................................................... 204
Epinephrine......................................................... 187, 215, 255
Epinine................................................................................ 255
Epioxy tetracycline, 4-.......................................................... 224
EPO dimer........................................................................... 294
EPO monomer...................................................................... 294
Equine Cytochrome c........................................................... 297
Erythritol, meso-.................................................................. 231
Erythromycin A.................................................................... 224
Erythromycin B.................................................................... 224
Erythromycin C.................................................................... 224
Erythromycin related compound N....................................... 224
Erythromycins...................................................................... 224
Estradiol...................................................................... 187, 269
Estriol.......................................................................... 187, 269
Estrogen.............................................................................. 269
Estrone........................................................................ 187, 269
Ethanol................................................................................ 229
Ethylbenzene....................................................................... 226
Ethylene glycol............................................................ 225, 365
Glycoprotein, -1 acid, Tryptic Digest.................................. 291

Glycylalanine (chiral)............................................................ 315
Glycylvaline (chiral).............................................................. 315
GMP, 5'-............................................................................... 164
GPC calibration............................................................ 167, 241
GTP, 5'-................................................................................ 164
Guanosine-5-monophosphate.............................................. 253
Haloperidol.......................................................................... 269
Heptanone, 2-...................................................................... 183
Hexadecanone, 2-................................................................ 183
Hexanophenone................................................... 183, 185, 269
Histidine (chiral)................................................................... 315
HMW aggregates................................................................. 294
HMW impurity.............................................................. 292, 294
HMX.................................................................................... 187
HPLC test mix...................................................... 183, 365, 366
Hydroxyalprazolam, -........................................................ 187

Hydroxybenzoic acid, p-....................................................... 195
Hydroxycortisone................................................................. 181
Hydroxycortisone, 17-.......................................................... 181
Hydroxydopamine, 6-........................................................... 255
Hydroxy 5-methyl benzaldehyde, 2-..................................... 269
Hydroxyprogesterone, 11-.................................................... 199
Hydroxyprogesterone, 11--............................................... 196
Hydroxyprogesterone, 17-............................................ 181, 187
Hydroxyprogesterone, 21-.................................................... 187
Hydroxyvitamin D2 (25-OH D2), 25-..................................... 189
Hydroxyvitamin D3 (25-OH D3), 25-..................................... 189
Ibuprofen..................................................................... 187, 257
Ig-A............................................................................. 292, 294
Ig-G..................................................................................... 294
Ig-G1 kappa dimer............................................................... 294
Ig-M.................................................................................... 292
Imipramine.................................................................. 172, 185
Indeno(1,2,3-cd)pyrene....................................... 166, 188, 196
Indomethacin....................................................................... 187
Insulin......................................................................... 298, 299
Iodide.................................................................................. 248
Isoleucine, D/L- (chiral)........................................................ 315
Isoprenes............................................................................. 219
Isoproterenol....................................................................... 215
Isorhamnetin....................................................................... 174
Kaempferol.......................................................................... 174
Kava Kava........................................................................... 194
Kawain................................................................................ 194
Kestose, 1-.......................................................................... 164
Ketamine (chiral).................................................................. 313
Ketones............................................................................... 183
Ketoprogesterone, 11-................................................. 196, 199
Labetolol.............................................................................. 170
Lactalbumin......................................................................... 298
Lactic acid........................................................................... 229
Lactoglobulin, -.................................................................. 294
Lactoglobulin A, -............................................................... 294
Lactoglobulin, PEGylated -................................................. 294
Laudanosine (chiral)............................................................ 313
Lauric acid........................................................................... 196
LDPE-C................................................................................ 241
Leptin.................................................................................. 298
Leucine, BOC- (chiral).......................................................... 315
Leucine, D/L- (chiral)........................................................... 315
Leucine, Z- (chiral)............................................................... 315
Levonordefrin...................................................................... 215
Lidocaine............................................................................. 174
Light corn syrup................................................................... 366
Phenomenex
Chromatogram Index

Phthalates........................................................................... 198
Phthalate esters................................................................... 198
Phthalate, di-n-butyl............................................................ 198
Phthalate, bis (2-Ethylhexyl)................................................. 198
Phthalate, di-n-octyl............................................................ 198
Phytane............................................................................... 219
Pindolol........................................170, 171, 185, 213, 250, 269
Pipecolic acid, D/L- (chiral).................................................. 315
Piromidic acid...................................................................... 197
Polar compounds................................................................. 250
Polyaromatic Hydrocarbons (PAHs)....................................... 188
Polybutadienes.................................................................... 219
Poly dT(12-18)............................................................. 275, 307
Polyethylene........................................................................ 241
Polyethylene glycol.............................................................. 218
Polyethylene oxide (PEO)...................................................... 219
Polymethyl methacrylates.................................................... 218
Methyl-leucine, - (chiral)................................................... 315

Methyl paraben.................................................... 196, 251, 254
Methyl-tryptophan, - (chiral).............................................. 315
Methysticin.......................................................................... 194
Metoprolol........................................................... 170, 171, 250
Metoprolol (chiral)................................................................ 320
Monuron.............................................................................. 166
Multi-grain cereal................................................................ 213
Myoglobin.................................................................... 290, 365
Myoglobin degradation profile.............................................. 290
Myristic acid........................................................................ 196
Nadolol................................................................................ 170
Nalidixic acid....................................................................... 197
Naphthalene........................ 166, 182, 183, 184, 188, 196, 218,
226, 239, 267, 268, 276, 365
Naphthylglycine (chiral)........................................................ 315
Naproxen............................................................................. 187
Napthol, 1-.......................................................................... 188
N-Dansylvaline, D/L- (chiral)................................................ 315
Neburon.............................................................................. 166
Nicotinamide............................................................... 201, 202
Nicotine....................................................................... 202, 202
Nicotinic acid............................................................... 201, 202
Niflumic acid....................................................................... 185
Nitrate................................................................. 244, 248, 365
Nitrite.................................................................. 244, 248, 365
Phenomenex
Poly-(-methyl styrene)....................................................... 219

Polynuclear Aromatic Hydrocarbons (PAHs).................. 166, 196
Polystyrenes................................................................ 219, 241
Polyvinyl butyral................................................................... 218
Poly-(2-vinylpyridine)........................................................... 220
Porcine insulin..................................................................... 299
Prednisolone........................................................ 185, 199, 269
Prednisolone-21-acetate..................................................... 199
Prednisone.......................................................................... 198
Preservatives....................................................................... 196
Procaine.............................................................................. 174
Progesterone....................................................................... 365
Proline, D/L- (chiral)............................................................. 315
Propham.............................................................................. 166
Propoxur.............................................................................. 166
Propranolol...........................170, 171, 250, 257, 338, 339, 345
Propyl paraben.................................................................... 196
Propyl paraben impurity....................................................... 196
Propylbenzene, n-................................................................ 241
Proteins........................................213, 292, 293, 294, 297, 298
Protriptyline................................................................. 185, 198
Pseudoephedrine......................................... 200, 251, 252, 254
Pyrene................................................................. 166, 188, 196
Pyridine............................................................... 178, 185, 257
Pyridoxine............................................................ 201, 202, 251
Pyrilamine................................................................... 170, 212
Pyruvic acid......................................................................... 255
Quercetin............................................................................. 174
Quinidine............................................................................. 269
Quinine................................................................................ 185
Rabeprazole (chiral)............................................................. 319
Raffinose............................................................................. 199
Ranitidine............................................................................ 189
RDX..................................................................................... 187
Ribitol.................................................................. 230, 231, 366
Riboflavin............................................................ 201, 202, 251
Ribonuclease............................................................... 292, 298
Ribose................................................................................. 232
RNA..................................................................................... 305
RNAse................................................................................. 298
Saccharides................................................................. 230, 231
Saccharose.......................................................................... 164
Salicin......................................................................... 231, 232
Salicylic acid........................................................................ 195
Appendices
HPLC
Nitroanisol, 2-...................................................................... 365

Nitrobenzene....................................................... 166, 187, 365
Nonanone, 2-....................................................................... 183
Non-polar basic compounds................................................ 178
Nordiazepam............................................................... 187, 257
Norepinephrine............................................................ 187, 255
Normetanephrine................................................................. 255
Nornicotine.......................................................................... 202
Norpinephrine...................................................................... 187
Nortriptyline................................................. 172, 185, 198, 269
Norvaline, D/L- (chiral)......................................................... 315
Norvaline, Z- (chiral)............................................................ 315
Nucleic acid bases............................................................... 164
Nucleotides.................................................................. 164, 251
Nystose............................................................................... 164
Octanophenone................................................................... 183
Oleandrin............................................................................. 189
Oleic acid............................................................................ 196
Oligonucleotide, mers.......................................................... 199
Oligonucleotide, 19 mer P-S................................................ 303
Oligonucleotide, 27 mer P-S................................................ 302
Oligos...................................................199, 275, 302, 303, 307
Oligosaccharides......................................................... 231, 366
Orange II.............................................................................. 188
Orange juice........................................................................ 230
Organic acids....................................................... 231, 244, 255
Organic compounds............................................................. 218
Ornithine, D/L- (chiral)......................................................... 315
Ovalbumin................................................... 293, 294, 298, 365
Oxalate................................................................................ 244
Oxalic acid................................................... 230, 231, 255, 366
Oxamyl........................................................................ 166, 188
Oxazepam................................................... 174, 187, 236, 257
Oxazepam (chiral)................................................................ 324
Oxolinic acid........................................................................ 197
Oxytetracycline.................................................................... 224
PABA........................................................................... 201, 202
Palmitic acid........................................................................ 196
Pantothenic acid.................................................................. 251
PEEK resin........................................................................... 241
PEG Modified Complex......................................................... 294
PEG Reagent........................................................................ 294
Pentadecanone, 2-............................................................... 183
Pentanone, 2-...................................................................... 183
Perylene.............................................................................. 167
Pharmaceuticals.................................................................. 269
Phenanthrene...................................................... 166, 188, 196
Phenol......................................................................... 196, 257
Phenol resin......................................................................... 241
Phenolic aldehyde resin....................................................... 218
Phenolic resins.................................................................... 218
Phenols, halogenated........................................................... 203
Phenylalanine, D/L- (chiral).................................................. 315
Phenylalanine, Z- (chiral)..................................................... 315
Phenylbutazone................................................................... 187
Phenylcyclohexanone, 2- (chiral).......................................... 320
Phenylephrine.............................................. 215, 251, 252, 254
Phenylglycine, D/L- (chiral).................................................. 315
Phenylpropanolamine.......................................... 251, 252, 254
Phenylserine, threo-3- (chiral).............................................. 315
Phosphate........................................................... 244, 248, 365
HPLC
Linoleic acid........................................................................ 196

Linolenic acid...................................................................... 196
Linuron................................................................................ 166
Loratadine........................................................................... 170
Lorazepam.......................................................................... 187
Lysine, D/L- (chiral).............................................................. 315
Lysozyme............................................................................ 298
Maleic acid.......................................................... 232, 252, 255
Malonic acid........................................................................ 255
Maltose........................................199, 229, 230, 231, 243, 366
Maltotriose.................................................................. 231, 243
Maltotriose hydrate.............................................................. 366
Mannitol...................................................................... 231, 232
Mannose............................................................. 230, 231, 366
Meclofenamic acid............................................................... 187
Medazepam......................................................................... 236
Melamine............................................................................ 188
Melezitose................................................... 199, 230, 231, 366
Metaproterenol.................................................................... 250
Metaraminol........................................................................ 215
Meteprolol........................................................................... 213
Methacrylate....................................................................... 244
Methacycline............................................................... 337, 339
Methiocarb.......................................................................... 188
Methionine, D/L- (chiral)...................................................... 315
Methocarbamol.................................................................... 340
Methomyl.................................................................... 166, 188
Methoxychlor....................................................................... 167
Methoxypolyethylene glycol................................................. 232
Methylacetophenone isomers.............................................. 204
Methylacetophenone, m-..................................................... 204
Methylacetophenone, o-...................................................... 204
Methylacetophenone, p-...................................................... 204
Methylaniline....................................................................... 178
Methyl-2-hydroxy benzaldehyde, 5-..................................... 185
CHROMATOGRAM INDEX
HPLC
399
Appendices
CHROMATOGRAM INDEX
Chromatogram Index
400
HPLC
HPLC
Saw Palmeto Berry.............................................................. 196

Serine, D/L- (chiral).............................................................. 315
Serine, Z- (chiral)................................................................. 315
Siduron................................................................................ 166
Sorbitol................................................................ 230, 231, 232
Sotalol................................................................................. 170
Squalene (C30).................................................................... 219
Stachyose.................................................................... 231, 232
Stearic acid......................................................................... 196
Steroids............................................................... 187, 196, 199
Stilbene oxide, trans-................................................... 340, 366
Sub P frag........................................................................... 200
Substance P........................................................................ 200
Succinic acid............................................... 230, 231, 232, 366
Sucrose....................................................... 199, 230, 231, 232
Sudan I................................................................................ 188
Sudan II............................................................................... 188
Sudan III.............................................................................. 188
Sudan IV.............................................................................. 188
Sudan Orange G.................................................................. 188
Sudan Red G........................................................................ 188
Sugars......................................................................... 199, 230
Sulfa drugs.......................................................................... 173
Sulfadimethoxine................................................................. 197
Sulfamerazine...................................................................... 173
Sulfamethoxazole................................................................ 173
Sulfanilamide....................................................................... 332
Sulfaquinoxaline.................................................................. 197
Sulfate................................................................. 244, 248, 365
Sulfathiazole................................................................ 173, 269
Sulfur.......................................................................... 167, 218
Sympathomimetic drugs...................................................... 215
Tartaric acid................................................. 230, 231, 255, 366
Temazepam................................................. 174, 187, 236, 257
Terfenadine.................................................................. 269, 341
Terphenyl, p-....................................................................... 166
Tetracaine............................................................................ 174
Tetracycline......................................................................... 224
Theobromine....................................................................... 174
Theophylline........................................................................ 174
Thiamine............................................................. 201, 202, 251
Thiamphenicol..................................................................... 197
Thiourea.............................................................. 253, 257, 365
Threonine, D/L- (chiral)........................................................ 315
Thymidine............................................................................ 251
Thymidine diphosphate........................................................ 251
Thymidine monophosphate.................................................. 251
Thymidine triphosphate....................................................... 251
Thymine.............................................................................. 253
Thyroglobulin............................................................... 292, 294
Tianeptine............................................................................ 185
Tolperisone (chiral)...................................................... 321, 322
Toluene..178, 182, 183, 184, 226, 239, 257, 267, 268, 276, 365
Toluic acid, p-...................................................................... 195
Transferrin................................................................... 294, 298
Tricyclic antidepressants...................................... 172, 185, 198
Tridecanone, 2-.................................................................... 183
Triethylene glycol................................................................. 232
Trifluoro-1-(9-anthryl)ethanol, R(-)-2,2,2-............................ 366
Trifluoro-1-(9-anthryl)ethanol, S(+)-2,2,2-........................... 366
Trinitrobenzene, 1,3,5-......................................................... 187

Trinitrotoluene, 1,3,5-.......................................................... 187
Trinitrotoluene, 2,4,6-.......................................................... 187
Tripelennamine............................................................ 170, 212
Triprolidine............................................185, 252, 269, 337, 339
Trypsin inhibitor................................................................... 298
Tryptic digest of BSA.................................................... 285, 288
Tryptic digest of -Casein.................................................... 273
Tryptic digest of Cytochrome c............................................. 200
Tryptophan, D/L- (chiral)...................................................... 315
Tyrosine, D/L- (chiral)........................................................... 315
UMP.................................................................................... 366
Uracil.....178, 181, 182, 183, 184, 239, 267, 276, 332, 365, 366
Urea.................................................................................... 232
Uridine......................................................... 292, 294, 365, 366
USP Method . ...................................................... 196, 224, 232
Valine, BOC- (chiral)............................................................. 315
Valine, D/L- (chiral).............................................................. 315
Valine, PTH- (chiral)............................................................. 315
Valine, Z- (chiral).................................................................. 315
Veterinary drugs.................................................................. 187
Vitamin B12 (Cyanocobalamin)............................................ 201
Vitamins...................................................................... 189, 251
Vitamins, water-soluble............................................... 201, 202
Xanthine.............................................................................. 174
Xylene, m-........................................................................... 365
Xylitol.................................................................................. 231
Xylose.......................................................................... 199, 231
Yangonin.............................................................................. 194
Phenomenex
Product Index
a
Abbreviations......................................................................380
Accessories and Consumables.. 9-34, 58-59, 98-114, 353-378
Accessories, GC............................................................. 98-114
Accessories, HPLC....................................................... 353-378
Achiral SFC................................................................. 331-333
Adapter Caps, SPE tube...................................................57, 59
Adapter, Fused Silica Capillary.............................................360
Adapter Kit, Strata Syringe and..............................................59
Adapter, PEEK..............................................................248, 360
Adapter, Reducing...............................................................360
Adapter, Solvent Pickup.........................................................14
Adjustable Pipettes................................................................20
Aeris HPLC Columns..................................... 272-274, 283-291
Affinity Columns..................................................................245
Agilent Technologies (HP) GC Systems, Parts for............ 99-104,
107-109, 112-114
Agilent Technologies HPLC Columns....................................264
Aluminum Seals for Vials.......................................................25
Amide Type Chiral Columns.................................................314
Aminex Alternative Column, Rezex.......................................228
Amino Acid Analysis.................................................... 278-282
Amino Acid Analysis Kits......................................................282
Amino Acids Analyzed for GC....................................... 278-282
Amino Acids Analyzed for LC/MS.................................278, 280
Amino Acids, underivatized..................................................315
Amino Columns....159, 160, 162, 179, 192, 193, 199, 208, 210,
222, 247, 264, 333, 342-343
Amylose-2, Lux Chiral Columns................................... 318-323
Anion-Exchange Test Mix.....................................................366
Appendices................................................................. 379-408
AQUA HPLC Columns...........................................................157
Aqueous SEC (GFC) Calibration Kits............................. 243, 367
Aqueous SEC (GPC) Calibration Kits............................. 243, 367
Aqueous SEC Test Mixes......................................................365
Asahipak HPLC Columns............................................. 158-159
ASTM Methods, GC Column Selection....................................65
Autosampler Compatibility Chart for Verex Vials.....................34
Autosampler Syringes, GC........................................... 108-110
Autosampler Vials............................................................ 21-34
Axia Packed Preparative HPLC..................................... 336-343
Phenomenex
Degasser, Mobile Phase Solvent..........................................359

Degassex DC-4400 HPLC Solvent Degasser........................359
Desalting Tubes...........................................................301, 306
Develosil HPLC Columns.............................................. 164-165
Direct-Connect Cartridge Holders................................205, 258
Disposable Centrifugal Filter..................................................12
Disposable In-Line Filter........................................................17
Disposable Syringes, Plastic..........................................12, 369
DNA/RNA Purification..................................................301, 310
Drugs of Abuse Analysis, GC Columns...................................94
e
Easy Seal Inlet Base Seal....................................................100
Eclipse XDB Columns..........................................................264
Efficiency Factor..................................................................285
End-Fittings, HPLC Column..................................................374
Engineered Self Crosslinking (ESC)........................................ 71
Environmental Analysis Columns, HPLC....................... 166-167
Environmental Sample Prep...................................... 48, 54, 55
EnviroSep HPLC Columns............................................ 166-167
EnviroSep-ABC GPC Columns..............................................167
EPA Methods, GC Column Selection.......................................64
EPA Methods, HPLC Column Selection......... 166, 187, 196, 248
EP Listings, HPLC Column Selection............................ 154-156
Equipment, HPLC........................................................ 356-359
Ether-linked Phenyl Column (Synergi Polar-RP)............ 249-251,
258-259, 331-333, 342-343, 347-348, 350-351
European Pharmacopeia Listings, HPLC Column Selection.........
154-156
Extraction Cartridges, On-line,SPE........... 36, 40, 41, 42, 49, 50
Extraction of Therapeutic Oligos.................................. 301-303
EZ:faast Amino Acid Analysis....................................... 278-282
Appendices
C6-Phenyl HPLC Column, Gemini......................... 168, 173-175

Cage, Five (5) Inch Zebron GC Columns.................................70
Calculations, HPLC...................................................... 383-386
Calibration Standards Kits, Polymer, HPLC................... 243, 367
Capacity Factor...........................................................383, 384
Capcell Pak HPLC Columns......................................... 161-162
Capillary Column Mini-Unions,GC..........................................99
Column Frits........................................................................374
Column Heater....................................................................357
Column Installation Instructions, GC............................. 381-382
Column Kits, GC, MultiResidue...............................................93
Column Loading Capacity....................................................384
Column Performance Check Standards, GC...........................98
Column Performance Check Standards, HPLC.............. 365-366
Column Protection Devices, HPLC..........................................15
Column Protection, GC.....................................................67, 68
Column Protection, HPLC.......... 15-18, 235-239, 317, 355, 386
Column Protection Systems, Chiral......................................317
Column Sealing Plug...........................................................363
Column Selection, GC...................................................... 62-66
Column Selection, HPLC.............................................. 146-156
Column Selector Installation Kit...........................................358
Column Selectors, HPLC......................................................358
Column Temperature Controllers.........................................357
Columns, BioSeparations............................................. 277-310
Columns, Chiral........................................................... 311-326
Columns, GC................................................................... 61-97
Columns, HPLC............................................................ 144-264
Columns, UHPLC......................................................... 265-276
Combination Oxygen/Moisture Traps....................................112
Combi-RP, Develosil Columns...................................... 164-165
Company History / Timeline........................... Inside Front Cover
Connectivity Kit for UHPLC...................................................362
Connectors, HPLC........................................................ 360-364
Cool-Lock Nut.............................................................101, 102
Cool-Lock Nut Installation Gauge......................................... 101
Core-Shell Technology........... 180-190, 266-275, 283-291, 307
Corporate Offices....................................................................1
Cosmosil HPLC Columns.....................................................163
Coupler, Column, HPLC..................................................18, 363
Crimp seals...........................................................................33
Crimp-Top Vials and Seals / Closures...............................24, 33
Curosil HPLC Columns.........................................................163
Cutters, Tubing............................................................372, 373
PRODUCT INDEX
Backpressure and Flow Rates Chart, HPLC..........................386

Backpressure Regulator......................................................364
Beliefs and Principles, Founding..............................................4
Bioethanol Analysis, GC Columns...........................................91
Bioethanol Fermentation.....................................................229
Biological Sample Cleanup....................................................56
BioSeparations............................................................ 277-310
BioSep-SEC-S HPLC Columns..................................... 292-295
Blood Alcohol Analysis, GC Columns......................................95
Bondapak Alternative HPLC Column, Bondclone.................160
Bondclone HPLC Columns...................................................160
Bonded-in Septa Caps..................................................... 27-32
BPX70 SGE GC Columns........................................................96
BPX90 SGE GC Columns........................................................96
BPX-Volatiles SGE GC Columns.............................................96
BTR-CW Quadrex GC Columns..............................................97
Bulk HPLC Media..................................259, 300, 323, 344-352
Bulk HPLC Media Selection Chart........................................348
Bulk Material, HPLC..............................259, 300, 323, 344-352
Bulk Material, Sepra............................................................352
Capillary Guard Columns..................................... 205, 258, 300

Capillary HPLC Columns.............................. 205, 214, 258, 300
Capillary PEEK HPLC Tubing........................................372, 373
Capillary Splitters, GC............................................................99
Capillary Stainless Steel Tubing...........................................372
Capillary Unions, GC..............................................................99
Capillary, Fused Silica, Adapter............................................360
Caps, Adapter, SPE Tube..................................................57, 59
Caps, Vial........................................................................ 25-33
Carbohydrate and Organic Acid Analysis HPLC Columns............
228-234
Carbohydrate and Sugar Alcohols Retention Times Chart.....234
Carbohydrate Test Mix.........................................................366
Cartridge Holders, Direct-Connect...............................205, 258
Cartridge Holders, MercuryMS.....................................205, 258
Cartridge Holders, SecurityGuard................................. 235-238
Cartridge Holders, SecurityGuard ULTRA..............................239
Cartridge Holders, Standard................................... 40, 205, 258
Cartridges, LC/MS.......................................................205, 258
Cartridges, On-line Extraction,SPE........... 36, 40, 41, 42, 49, 50
Cation-Exchange Test Mix...................................................366
CD Vial................................................................ 24, 27, 28, 32
CD-Ph Chiral Columns.........................................................313
Cellulose-1, Lux Chiral Columns.................................. 318-323
Centrifugal Filters..................................................................12
Ceramic Scoring Wafers......................................................114
Ceramospher Chiral Columns..............................................313
Certification, Vials............................................................ 22-23
Certified PLUS Vial Caps..................................................27, 29
Certified PLUS Screw-Top Vials, Caps, and Kits................27, 29
Certified PLUS Shell Vials.......................................................32
Check Standards, GC Column Performance...........................98
Check Standards, HPLC Column Performance.............. 365-366
Chiller/Heater, HPLC Column................................................357
ChiraDex Chiral Cartridges...................................................324
Chiral CD-Ph HPLC Columns................................................313
Chiral Chromatography Columns................................. 311-326
Chiral Columns Protection Systems.....................................317
Chiral HPLC Applications............................................. 312-326
Chiral LC Column Types.......................................................312
Chiral LC Stationary Phase Classification Diagram...............312
Chiral Method Development Kits..........................................316
Chiral Method Screening Kits...............................................323
Chiral Screening and Separation Services................... 6-8, 322
Chiral SFC........................................................... 328-330, 333
Chiral Test Mixes.................................................................366
Chirex Chiral Columns......................................... 312, 314-316
Chirex Column Selection Guide............................................314
Chromatogram Index, GC............................................. 390-396
Chromatogram Index, HPLC......................................... 397-400
Chromatographic Parameters..............................................384
Chromolith HPLC Columns...................................................163
ChromTRAC Knobs..............................................................377
Clarity BioSolutions for DNA/RNA Purification.............. 301-310
Clarity Desalting Tubes................................................301, 306
Clarity Oligo-MS HPLC Columns.......................... 301, 307, 310
Clarity Oligo-RP HPLC Columns........................... 301, 308, 310
Clarity Oligo-WAX HPLC Columns........................ 301, 309, 310
Clarity OTX Extraction Kits................................... 301, 302, 303
Clarity QSP Cartridges and Well Plates................. 301, 304, 305
Cleaning Kit, Flame Detector Jet..........................................114
Cleaning Kit, Injection Port, GC.............................................114
Collection Plates, SPE............................................................59
Columbus HPLC Columns....................................................163
Column Assemblies, Stainless Steel.....................................374
Column Characterization Probes..........................................384
Column Chiller/Heater.........................................................357
Column Cleaning Procedures, HPLC.....................................386
Column Coupler, Analytical............................................18, 363
Column Coupler, Onyx.................................................214, 363
Column Coupler, Prep............................................ 18, 237, 363
Column Efficiency, HPLC..............................................383, 385
Column End-fittings.............................................................374
Column Flow Rates & Backpressures..........................383, 386
401
Product Index
f
Appendices
PRODUCT INDEX
Fast GC Columns...................................................................69
Fast LC Columns.........................................................205, 258
Ferrule Remover Tool Kit......................................................114
Ferrule Selection Guides, GC...............................................102
Ferrules, GC................................................................ 102-103
Ferrules, HPLC.................................................... 360, 361, 363
Filter Membranes............................................................13, 15
Filter Membrane Selection Guide...........................................10
Filter Reservoir Cap...............................................................14
Filters................................................................................ 9-18
Filters, Centrifugal.................................................................12
Filters, Inlet...........................................................................16
Filters, In-line.................................................................. 17-18
Filters, Syringe................................................................ 10-12
FilterSys Mobile Phase Filtration System...............................13
Filtration Apparatus Cross-Reference Chart...........................13
Filtration Plate, 96-Well.........................................................59
Filtration Products............................................................. 9-18
Fingertight Fittings...................................................... 360-364
Fingertight Male Nuts.................................................. 360-364
Fitting Material Maximum Temperature Ratings (C) Table...360
Fittings, GC..........................................................................101
Fittings, HPLC.............................................................. 360-364
Fittings, RheFlex..................................................................377
Fittings, Ultra-High Performance LC............................. 361-362
Fitting, Tightening Tool, Sure-Lok................................. 361-362
Fitting Wrench, Rheodyne............................................373, 378
Five (5) Inch Cage GC Columns..............................................70
Flame Detector Jet Cleaning Kit..........................................114
Flangeless Fittings..............................................................364
Flash Analysis/Large Volume Cleanup (Giga Tubes).... 36, 40-46,
48-55
Flex-Coil Capillary PEEK Tubing...........................................373
Fluid Processors, HPLC........................................................358
FocusLiners, Tapered................................................... 104-106
forte BPX70...........................................................................96
forte BPX90...........................................................................96
forte BPX-Volatiles.................................................................96
forte HT8...............................................................................96
forte SolGel-WAX...................................................................96
Founding Beliefs and Principles...............................................4
Frit Pore Size Selection Guide..............................................374
Frit Size Selection Guide......................................................374
Frits....................................................................................374
Fused Silica Capillary Adapter.............................................360
Fusion-RP Columns, Synergi................ 249, 252-253, 258-259,
342-343, 350-351
402
g
Gas Chromatography, GC............................................... 61-143
Gas Purifiers........................................................................112
GASTIGHT Priming Syringes................................................369
Gastight Syringes.........................................108-111, 368-370
GC Accessories............................................................ 353-378
GC Capillary Nut..................................................................101
GC Column Applications.............................................. 115-143
Environmental......................................................... 115-124
Food, Flavors, Fragrances....................................... 130-132
Food Safety....................................................................133
Industrial Chemicals............................................... 134-138
Petroleum / Fuel.................................................... 139-143
Pharmaceuticals..................................................... 128-129
Toxicology / Drugs of Abuse.................................... 125-127
GC Column Installation Instructions............................. 381-382
GC Column MS Certification, Zebron....................................382
GC Column Selection by Manufacturer...................................62
GC Column Selection Chart....................................................63
GC Column Selection, ASTM..................................................65
GC Column Selection, EPA.....................................................64
GC Column Selection, USP.....................................................66
GC Columns.................................................................... 61-97
EZ:faast.................................................................. 278-282
Quadrex............................................................................97
SGE..................................................................................96
Zebron........................................................................ 61-95
GC Columns Check Standards...............................................98
GC Columns Cross Reference Chart.......................................62
GC Columns, Fast..................................................................69
GC Columns, Five (5) Inch Cage.............................................70
GC Fittings...........................................................................101
GC Guard Columns..........................................................67, 68
GC Injection Syringes...................................................108-111
GC Integrated Guard Columns...............................................67
Gemini / Gemini-NX HPLC Columns..............168-175, 337-339,
342-343, 344-348, 350-351
GFC / Organic SEC Calibration Kits......................................367
GFC/SEC Columns.158-159, 225, 240, 243, 260-261, 292-295
GPC / Aqueous SEC Calibration Kits.....................................367
GPC/SEC Columns.........................158-159, 216-221, 240-242
Giga Tubes, SPE.............................................. 36, 40-46, 48-55
Gold GC Inlet Base Seal.......................................................100
Graphite Ferrules......................................................... 102-103
Guard Cartridge Holders.......................162, 165, 235-239, 355
Guard Cartridge System, HPLC.............190, 235-239, 276, 355
Guard Cartridge System, Onyx.............................................214
Guard Cartridge System, STAR-ION......................................248
Guard Columns and Kits, GC............................................67, 68
Guard Columns, Capillary, HPLC.......................... 205, 258, 300
Guard Columns, HPLC..........159, 160, 167, 176, 221, 223, 225,
234-239, 242, 244, 245, 260
Guard Columns, Presaturator...............................................355
Guard Hardware Kits, ZORBAX.............................................264
Guardian System...................................................................67
Guardian, Integrated Guard Columns.....................................67
GuideRight Injection Hole Septa...........................................107
h
Hamilton GASTIGHT Syringes...............108, 110, 111, 368-369
Hamilton HPLC Columns......................................................176
Hamilton HPLC Injector Syringes.........................................368
Hamilton Syringes........................................108-111, 368-369
Headspace Vials, Crimp Top Seals / Closures.........................33
Heater, HPLC Column..........................................................357
Heater/Chiller, HPLC Column...............................................357
HIBAR HPLC Columns..........................................................209
High Performance Liquid Chromatography Columns.... 144-350
High Speed Technology (HST) Columns........................205, 258
High-Throughput 96-Well Plates............... 36, 38, 40-46, 49-55
High-Throughput Preparative Columns, HPLC.............. 335-350
High-Throughput Protein Precipitation, SPE...........................56
High-Throughput Screening, SPE...........................................38
HILIC Columns...................... 180, 186-188, 190, 193, 201-202,
205-207, 331, 333, 342-343
HILIC Phase Test Mix...........................................................365
HPLC Calculations....................................................... 383-386
HPLC Cartridges, LiChrospher..............................................208
HPLC Cartridges, MercuryMS......................................205, 258
HPLC Cartridges, Purospher................................................209
HPLC Cartridges, Superspher..............................................209
HPLC Column Check Standards................................... 365-366
HPLC Column End-Fittings...................................................374
HPLC Column Protection.......... 15-18, 235-239, 317, 355, 386
HPLC Columns Cross Reference Charts............... 148-150, 160,
234, 295
HPLC Column Selection Tree...............................................146
HPLC Column Selection, By Application...............................147
HPLC Column Selection, By EP Listing......................... 154-156
HPLC Column Selection, By Manufacturer.................... 148-150
HPLC Column Selection, By Separation Mode......................151
HPLC Column Selection, By USP Listing....................... 152-153
HPLC Column Selection, Shodex..........................................240
HPLC Columns............................................................ 144-264

Aeris..................................................... 272-274, 283-291
Aqua............................................................................157
Asahipak.............................................................. 158-159
Axia..................................................................... 336-343
BioSep................................................................. 292-295
Bondclone...................................................................160
Capcell Pak.......................................................... 161-162
Ceramospher Chiral.....................................................313
ChiraDex......................................................................324
Chiral CD-Ph................................................................313
Chirex.................................................................. 314-316
Chromolith...................................................................163
Clarity.................................................................. 301-310
Columbus....................................................................163
Cosmosil......................................................................163
Curosil.........................................................................163
Develosil.............................................................. 164-165
EnviroSep............................................................ 166-167
EZ:faast............................................................... 278-282
Gemini / Gemini-NX............................................. 168-175
Hamilton......................................................................176
Hypercarb....................................................................317
HyperClone.......................................................... 177-178
Hypersil.......................................................................179
Hypersil BDS................................................................179
IB-Sil...........................................................................179
Inertsil.........................................................................179
Jupiter................... 296-300, 342-343, 347, 348, 350-351
Kinetex................................................. 180-190, 266-271
Kromasil......................................................................191
LiChrosorb...........................................................192, 209
LiChrospher................................................. 192, 208, 209
Luna.................................................................... 193-207
Lux...................................................................... 318-323
Maxsil..........................................................................208
Merck KGaA......................................................... 208-209
Nucleodex....................................................................324
Nucleosil......................................................................210
Nucleosil Chiral............................................................324
Onyx.................................................................... 211-214
Partisil.................................................................215, 263
PartiSphere..................................................................263
Phenogel..............................................................216-221
PhenoSphere...............................................................222
PhenoSphere-NEXT.....................................................222
Polymer Labs...............................................................223
PolymerX.....................................................................224
PolySep-GFC-P............................................................225
PrimeSphere................................................................225
Prodigy................................................................ 226-227
Purospher....................................................................209
Resolvosil....................................................................324
Rezex.................................................................. 228-234
SecurityGuard...................................................... 235-238
SecurityGuard ULTRA...................................................239
Selectosil.....................................................................240
Shodex................................................................ 240-245
Shodex Chiral..............................................................325
SphereClone........................................................ 246-247
Spherex.......................................................................247
Spherisorb...................................................................247
STAR-ION A300............................................................248
Sumichiral OA..............................................................326
Superspher..........................................................192, 209
Synergi................................................................ 249-259
TSK-GEL...............................................................260-261
Ultracarb......................................................................262
Ultrasphere..................................................................262
Ultremex......................................................................262
Ultron..........................................................................325
Vydac..........................................................................262
Whatman.....................................................................263
Whelk-O 1...................................................................324
ZORBAX.......................................................................264
Phenomenex
Product Index
HPLC Guard Columns...........159, 160, 167, 176, 221, 223, 225,
234-239, 242, 244, 245, 260
HPLC Material Sorbent Characteristics........................ 388-389
HPLC Reversed Phase Method Development............... 384-385
HPLC System Test Kit..........................................................355
HST Columns..............................................................205, 258
HT8 SGE GC Columns............................................................96
Hydraulic Piston Compression.............................................336
Hydrocarbon Traps..............................................................112
Hydro-RP Columns, Synergi.................. 249, 254-255, 258-259,
342-343, 348, 350-351
Hypercarb HPLC Columns....................................................317
HyperClone HPLC Columns.......................................... 177-178
Hypersil Alternative HPLC Columns, HyperClone.......... 177-178
Hypersil BDS HPLC Columns...............................................179
Hypersil HPLC Columns.......................................................179
Kinetex HPLC Columns................................. 180-190, 266-271

Kit, Column Selector Installation..........................................358
Kit, Ferrule Remover Tools...................................................114
Kit, Flame Detector Jet Cleaning..........................................114
Kit, HPLC System Test.........................................................355
Kit, Injection Port Cleaning...................................................114
Kit, Method Validation, Onyx Monolithic C18........................214
Kit, SecurityGuard....................................................... 236-238
Kit, Strata Protein Precipitation..............................................56
Kit, Tubing, PEEK.................................................................372
Kits, Amino Acid Analysis............................................. 278-282
Kits, Chiral Method Development.........................................316
Kits, Chiral Method Screening..............................................323
Kits, Extraction, Clarity OTX......................................... 301-303
Phenomenex
LabClear OxiClear Gas Purifiers...........................................112

Large Volume Cleanup/Flash Analysis (Giga Tubes)................36,
40-46, 48-55
LC/MS Columns and Cartridges...................................205, 258
Letter from CEO and President................................................2
LiChroCART Cartridge End-fitting.........................................208
LiChrosorb HPLC Columns...........................................192, 209
LiChrospher HPLC Cartridges..............................................208
LiChrospher HPLC Columns.........................................192, 209
Ligand Exchange Type Chiral Columns................ 312, 324, 326
Limited Volume Vial Inserts.................................. 24, 29, 30, 31
Limited Volume Vials....................................................... 24-32
Linear GPC Columns............................................ 220-221, 242
Liners, GC Injection Port Inlet....................................... 104-106
Loops, HPLC Sample Injector....................................... 375, 377
Low-Dispersion Sample Injector..........................................376
Luer Stopcocks.....................................................................58
Luna HPLC Columns...... 193-207, 331-333, 337-339, 342-350
Lux Chiral Columns.......312, 318-323, 329-330, 333, 339-344,
348, 350-351
Lux Method Screening Kits..................................................323
m
Manifold, 96-Well Plate................................................... 58-59
Manifold, Vacuum, SPE.................................................... 57-59
Mass Spec Quality (MSQ) Vial Kits........................22-23, 27, 29
Material Sorbent Characteristics, Aqueous SEC/GFC............389
Material Sorbent Characteristics, HPLC....................... 388-389
Material Sorbent Characteristics, Non-Aqueous SEC/GPC....389
Maximum Temperature Ratings, Fitting and Tubing Materials.....
371
Max-RP Columns, Synergi............ 249, 256-259, 339, 342-343,
347-351
Maxsil HPLC Columns.........................................................208
Membranes, Filter...........................................................13, 15
Merck KGaA, Darmstadt, Germany HPLC Columns....... 208-209,
324
MercuryMS LC/MS Columns and Cartridges................205, 258
Merlin Microseal Septum.....................................................113
Method Development, HPLC Reversed Phase............... 384-385
Method Development Kits, Chiral . ......................................316
Method Development Kits, SPE..............................................38
Method Development Web Tool, SPE......................................37
Method Screening, Chiral....................................................323
Method Validation Kit, Onyx HPLC Columns.........................214
Microsampling Vials...................................................24-28, 31
Mini-Unions, GC Capillary Column.........................................99
Miscibility Table, Solvent......................................................387
Mission Statement..................................................................4
Mixed-Mode Strong Cation Exchange, SPE.......... 39, 41, 45, 53
Mixed-Mode Strong Anion Exchange, SPE................. 39, 43, 53
Mixed-Mode Weak Anion Exchange, SPE................... 39, 44, 53
Mixed-Mode Weak Cation Exchange, SPE........................39, 42
Mixing Tee, PEEK.................................................................364
Mobile Phase Accessories............................................... 13-16
Mobile Phase Filtration System..............................................13
Mobile Phase Inlet Filters......................................................16
Mobile Phase Pickup Adapter................................................14
o
Offices, Expansion, Photos.......................................................1
Oligo-MS HPLC Columns..................................... 301, 307, 310
Oligonucleotide Purification......................................... 301-310
Oligo-RP HPLC Columns...................................... 301, 307, 310
Oligosaccharide Standard Test Mix......................................366
Oligo-WAX HPLC Columns................................... 301, 309-310
On-Line Extraction Cartridge, SPE Format............ 36, 40, 41, 42,
49, 50
Onyx Column Coupler..................................................214, 363
Onyx HPLC Columns.................................................... 211-214
Onyx Monolithic Normal Phase Test Mix...............................365
Onyx Monolithic Reversed Phase Test Mix...........................365
Orbit Precision Adjustable Pipette..........................................20
Organic Acid Standard Test Mix...........................................366
Organic Acids Columns................. 176, 228-234, 244, 254-255
Organic SEC (GPC) Calibration Kits.............................. 243, 367
O-Rings, Sealing..................................................................107
OTX Extraction Kits, Clarity.................................. 301, 302-303
OxiClear Gas Purifiers..........................................................112
Oxygen/Moisture Traps........................................................112
p
Partisil HPLC Columns.................................................215, 263
PartiSphere HPLC Columns.................................................263
Peak Asymmetry.................................................................383
PEEK Mixing Tee..................................................................364
PEEK Tubing................................................................ 371-373
PEEK Tubing Kit...................................................................372
PEEKsil Tubing.............................................................362, 372
Pentafluorophenyl (PFP) Columns
Kinetex PFP.......................... 180, 186, 187, 190, 270-271
Luna PFP(2).................................. 193, 203-207, 342-343
Peptide Mapping and Purification
Aeris HPLC Columns.............................. 272-274, 283-291
Jupiter HPLC Columns.......................................... 296-300
PerkinElmer GC Systems, parts for..............................105, 109
PFP Columns........................ 180, 186, 187, 190, 193, 203-207,
270-271, 342-343
Phase Selection, GC........................................................ 62-66
Phase Selection, HPLC................................................ 146-156
Appendices
Nacalai Tesque HPLC Columns............................................163

Narrow Bore GPC Columns.................................. 220-221, 242
Needle Gauge Dimensions, Syringe.....................................108
Needle / Needle Connection Styles, Syringe.........................108
Needle Point Styles, Syringe................................................108
Needle Port Cleaner.....................................................375, 378
Needle Port Tube Assembly.................................................376
Needles, Removable, Syringe.......................108-111, 368-370
Nomura Chemical Co. HPLC Columns.......................... 164-165
Normal Phase Test Mix........................................................365
Nucleodex Chiral Columns...................................................324
Nucleosil Chiral Columns.....................................................324
Nucleosil HPLC Columns.....................................................210
Nut, Cool-Lock, GC..............................................................101
Nut, HPLC.................................................................... 360-364
Nut, Installation, GC.............................................................101
Nut, Male, PEEK........................................................... 361-363
Jupiter HPLC Columns... 296-300, 342-343, 347, 348, 350-351

Jupiter Proteo HPLC Columns............... 296-300, 342-343, 348,
350-351
Mobile Phase Recycler........................................................356

Mobile Phase Reservoirs and Caps........................................14
Mobile Phase Solvent Degasser...........................................359
Moisture, Oxygen and Hydrocarbon Gas Traps/Purifiers.......112
Monolithic Silica HPLC Columns.................................. 211-214
MR-1, MR-2, Zebron GC Columns.................................... 92-93
MS Certification, Zebron GC Columns..................................382
MultiResidue GC Columns and Kits.................................. 92-93
PRODUCT INDEX
IB-Sil HPLC Columns...........................................................179

Impact Protein Precipitation Plate..........................................56
Indicating Glass Moisture Traps...........................................112
Indicating Oxygen Traps......................................................112
Inertsil HPLC Columns.........................................................179
Inferno, Zebron GC Columns... 62, 67, 69, 70, 73, 78-79, 81, 83
Injection Hole Septa............................................................107
Injection Port Cleaning Kit....................................................114
Injection Port Inlet Liners............................................. 104-106
Injector Accessories, Rheodyne, HPLC......................... 375-378
Injector, HPLC Sample................................................. 375-378
Injector Septa Plug..............................................................107
Injector Syringes, HPLC...............................................368, 370
Inlet Base Seals...................................................................100
Inlet Filters, Metal-Free/Biocompatible..................................16
Inlet Filters, Stainless Steel....................................................16
Inlet Septa...........................................................................107
In-Line Filter, Disposable.......................................................17
In-Line Filters, Metal-Free/Biocompatible (Analytical)............17
In-Line Filters, Metal-Free/Biocompatible (SemiPrep)............18
In-Line Filters, Stainless Steel (Analytical)..............................17
In-Line Filters, Stainless Steel (PREP)....................................18
Inserts,Limited Volume, Vials............................... 24, 29, 30, 31
Installation Gauge, Cool-Lock Nut........................................101
Installation Instructions, GC Column.............................381-381
Installation Nuts, GC............................................................101
Integrated Guard Columns, GC...............................................67
Ion Chromatography Columns............................. 176, 244, 248
Ion-Exchange Chromatograhy Columns....................... 158-159,
162, 193, 200, 210, 215, 222, 228, 240, 245, 263, 309
Kits, GC Guard Columns........................................................69

Kits, Guard Hardware, ZORBAX............................................264
Kits, MultiResidue GC Columns..............................................93
Kits, Polymer Calibration Standards............................. 243, 367
Kits, RheBuild......................................................................378
Kits, Sample Preparation Method Development.....................38
Kits, Screening, LC/MS Cartridges...............................205, 258
Kits, Valve Repair, Fluid Processors......................................358
Kits, Vial...............................................................26-29, 31-33
Kromasil HPLC Columns......................................................191
KrudKatcher Classic Disposable Pre-Column Filter................17
KrudKatcher Ultra In-Line Filter.............................................17
403
Product Index
Appendices
PRODUCT INDEX
r
Phase Selection, SPE................................................. 39, 47-48
PHAT Phases, GC Columns....................................................97
Phenex Centrifugal Filters......................................................12
Phenex Pipet Tips..................................................................20
Phenex Syringe Filters..................................................... 10-12
Phenogel GPC/SEC Columns.........................................216-221
Phenogel Solvent and Temperature Compatibility Chart.......216
phenoLogix................................................................. 6-8, 322
PhenoSphere HPLC Columns...............................................222
PhenoSphere-NEXT HPLC Columns.....................................222
Phenyl Phase Selectivity Comparison..................................226
Phenyl, C6- Phase, Gemini.................................. 168, 173-175
Phenyl Ether-Linked HPLC Columns, Synergi Polar-RP........ 249251, 258-259, 331-333, 342-343, 347-348, 350-351
Phenyl-Ethyl HPLC Columns, Prodigy........................... 226-227
Phenyl-Hexyl HPLC Column, Kinetex............ 180, 186, 187, 190,
270-271
Phenyl-Hexyl HPLC Column, Luna.................193, 197, 205-207,
342-343, 348, 350
Photos of Offices.....................................................................1
Pipettes.................................................................................20
Pipette Stand........................................................................20
Pipet Tips..............................................................................20
PLgel GPC Columns.............................................................223
PLOT, GC Columns.................................................................97
PLRP-S Columns.................................................................223
Plugs, Column Sealing.........................................................363
Polar Embedded C18 Column (Synergi Fusion-RP)..............249,
252-253, 258-259, 342-343, 350-351
Polar Endcapped C18 Column (Synergi Hydro-RP)...............249,
254-255, 258-259, 342-343, 348, 350-351
Polar-RP Columns, Synergi............249-251, 258-259, 331-333,
342-343, 347-348, 350-351
Polyimide Resin.....................................................................99
Polymer-Based Reversed Phase HPLC Columns..........224, 245
Polymer Calibration Standards Kits.............................. 243, 367
Polymer Labs Columns........................................................223
Polymer Tubing Cutter.................................................372, 373
PolymerX HPLC Columns.....................................................224
PolymerX RP-1 Test Mix......................................................365
Polysaccharide-Based Chiral Stationary Phases.......... 318-323
PolySep-GFC-P Columns.....................................................225
Power Line Cords................................................................358
Preparative Columns....................................336-343, 347-350
Prep Column Coupler....................................................18, 363
Prep Columns...............................................336-343, 347-350
PREP Guard Cartridge Holder............................... 237, 238, 342
PREP In-line Filter..................................................................18
Prep Media.................................................................. 344-352
Presaturator Guard Columns...............................................355
PrimeSphere HPLC Columns...............................................225
Priming Syringe...................................................................369
Priority Pollutants Analysis...................................................166
Probes For Column Characterization....................................384
Process Chromatography............................................ 344-352
Prodigy HPLC Columns................................................ 226-227
Product Index.............................................................. 401-405
Protein and Peptide Analysis
Aeris HPLC Columns..............................................283-291
BioSep HPLC Columns.......................................... 292-295
Jupiter HPLC Columns.......................................... 296-300
Protein Precipitation Plates....................................................56
Proteins and Nucleic Acids Columns.................... 164, 245, 296
Proteo, Jupiter HPLC Columns......................296-297, 299-300
Purifiers, Gas.......................................................................112
Purospher HPLC Cartridges.................................................209
q
QC Standards, GC..................................................................98
QC Standards, HPLC.................................................... 365-366
Qsert Vial................................................................... 24, 28, 31
QSP Cartridges and 96-WP, Clarity....................... 301, 304-305
Quadrex GC Columns............................................................97
404
Rapid Protein Precipitation, Strata Impact..............................56

Recycler, Mobile Phase........................................................356
Reducing Adapter................................................................360
Regulator, Backpressure......................................................364
Replacement Valve Caps.......................................................14
Reservoir and Valve Cap Assembly........................................14
Resin, Polyimide....................................................................99
Resolution, HPLC................................................. 383, 384, 385
Resolvosil Chiral Columns...................................................324
Retention Factor..........................................................384, 385
Retention Gaps, GC...............................................................68
Reversed Phase Method Development......................... 384-385
Reversed Phase Test Mixes......................................... 365-366
Rezex HPLC Columns.................................................. 228-234
RheBuild Kits.......................................................................378
RheFlex Fitting Accessory....................................................377
RheFlex Fittings...................................................................377
Rheodyne Fitting Wrench.............................................373, 378
Rheodyne Sample Injectors......................................... 375-378
Rheodyne Switching Valve/Injector......................................376
Rotor Seals.................................................................375, 378
Rx Series HPLC Columns.....................................................264
s
Sample Cleanup, Biological...................................................56
Sample Injector Loops................................................. 374, 377
Sample Injectors......................................................... 374-378
Sample Preparation......................................................... 35-59
Sample Preparation Accessories...................................... 57-59
Sample Preparation Formats.................................................36
Sample Preparation Method Development Kits......................38
Sample Preparation Solutions................................................37
Sample Preparation Technical Resources..............................37
Sample Preparation Techniques.............................................36
Scoring, Wafers, Ceramic....................................................114
Screening Kits, LC/MS Cartridges................................205, 258
Screw-Top Vials, Caps, and Kits............................24, 27-32, 34
Seals, Aluminum, Vial............................................................25
Sealing Mats, SPE.................................................................59
Sealing Plugs, HPLC Column...............................................363
Sealing O-Rings..................................................................107
Sealing Tape..........................................................................59
Seals, Rotor.................................................................375, 378
SEC/GFC Columns.158-159, 225, 240, 243, 260-261, 292-295
SEC/GPC Columns.........................158-159, 216-221, 240-242
SecurityGuard............................................................. 235-239
SecurityGuard Accessories.......................................... 236-237
SecurityGuard Analytical Holder Kit..............................236, 238
SecurityGuard Cartridges............................................. 235-239
SecurityGuard Preparative Holders...................... 237-238, 342
SecurityGuard Semi-Preparative Holder....................... 237-238
SecurityGuard ULTRA Cartridge Holder and Cartridge System....
239
Selectivity Factor.........................................................384, 385
Selectosil HPLC Columns.....................................................240
Separation Factor................................................................383
Sepra Bulk Sorbents............................................................352
Septa, GC Inlet............................................................107, 113
Septa Sizes by GC Instrument................................................99
Septa, Vial.............................................................................30
Septum, Merlin Microseal....................................................113
Services.............................................................................. 5-8
SFC (Supercritical Fluid Chromatography).................... 327-333
SGE Gastight Luer Lock Syringes.........................................370
SGE GC Columns...................................................................96
SGE Syringes....................................................... 109-111, 370
Shell Vials.............................................................................32
Shimadzu GC Systems, parts for.......... 101, 103, 105, 107, 109
Shimadzu Injector Septa Plug..............................................107
Shinwa Chemical Industries, Ltd. Chiral Columns.................325
Shiseido Co, Ltd. Chiral Columns.........................................313
Shiseido Co, Ltd. HPLC Columns.................................. 161-162
Shodex Chiral Columns.......................................................325
Shodex HPLC Columns................................................ 240-245
Showa Denko K.K. HPLC Columns........ 158-159, 240-245, 325

Silicone Rubber Inlet Septa..................................................107
SilTite Ferrules............................................................ 102-103
SilTite Mini-Unions................................................................99
Snap Caps.............................................................................26
Snap-Top Vials and Seals / Closures.................................24,26
SolGel-WAX SGE GC Columns................................................96
Solid Phase Extraction (SPE)............................................ 35-59
Solvent and Temperature Compatibility Table for GPC Columns..
217
Solvent Degasser................................................................359
Solvent Filtration System.......................................................13
Solvent Inlet Filters................................................................16
Solvent Miscibility Table......................................................387
Solvent Pickup Adapter..........................................................14
Solvent Polarity Table..........................................................387
Solvent Recycler..................................................................356
Solvent Reservoir and Reagent Bottles..................................14
Solvent Reservoirs/Bottle Filter Cap.......................................14
Solvent Saver Inlet Filter - Metal Free ...................................16
Solvent Saver Inlet Filter - Stainless Steel..............................16
Solvent Switching Considerations for GPC Columns.............217
SolventTrak Mobile Phase Recycler......................................356
Spanners.............................................................................373
SPE (Solid Phase Extraction)............................................ 35-59
SPE Method Development Tools................................. 36-39, 48
SPE Tubes....................................................................... 36-56
SPE Vacuum Manifolds.................................................... 57-59
SphereClone HPLC Columns........................................ 246-247
Spherex HPLC Columns.......................................................247
Spherisorb Alternative Columns, SphereClone............. 246-247
Spherisorb HPLC Columns...................................................247
Splitters................................................................................99
StableBond Columns...........................................................264
Stacking Rings, SecurityGuard.............................................236
Stainless Steel GC Inlet Base Seals......................................100
Standards, GC.......................................................................98
Standards, HPLC......................................................... 365-366
Standards, Polymer Calibration Kits............................. 243, 367
STAR-ION A300 HPLC Columns...........................................248
STAR-ION A300 Test Mix......................................................365
STAR-ION Guard Cartridge System......................................248
Stators, Valve......................................................................378
Stopcocks, Luer, SPE.............................................................58
Storage Conditions for Silica-Based HPLC Columns.............386
Strata 96-Well Plates................................................ 36, 48-55
Strata Giga Tubes...................................................... 36, 48-55
Strata Impact Precipitation Plates..........................................56
Strata On-Line Extraction Cartridge.................................49, 50
Strata Ion-Exchange SPE Sorbents............................ 47, 48, 52
Strata Mixed-Mode SPE Sorbents.............................. 47, 48, 53
Strata Normal Phase SPE Sorbents............................ 47, 48, 51
Strata Reversed Phase SPE Sorbents................... 47, 48, 49, 50
Strata Sample Preparation Products................................ 35-59
Strata Solid Phase Extraction (SPE).................................. 35-59
Strata SPE (Solid Phase Extraction).................................. 35-59
Strata SPE Method Development....................36, 38, 39, 47-48
Strata Specialty SPE Sorbents............................. 47, 48, 54, 55
Strata Syringe and Adapter Kit...............................................59
Strata Teflon Giga Tube..........................................................55
Strata Tubes.................................................................... 36-56
Strata-X.................................................................... 36, 38-46
Strata-X 96-Well Plates........................................36, 38, 40-46
Strata-X Giga Tubes................................................... 36, 40-46
Strata-X Method Development Kits........................................38
Strata-X On-Line Extraction Cartridge........................ 36, 40-42
Strata-X Polymeric (SPE) Sorbents............................. 36, 38-46
Strata-X Teflon Tubes............................................................40
Strata-X Tubes.......................................................... 36, 38-46
Strata-X-A................................................................. 38, 39, 43
Strata-X-AW.............................................................. 38, 39, 44
Strata-X-C................................................................. 38, 39, 40
Strata-X-CW.............................................................. 38, 39, 42
Strata-X Drug B...............................................................39, 45
Strata-X Drug N...............................................................39, 46
Strata-XL........................................................................39, 40
Strata-XL-A.....................................................................39, 43
Phenomenex
Product Index
z
Strata-XL-AW..................................................................39, 44
Strata-XL-C.....................................................................39, 40
Strata-XL-CW..................................................................39, 42
Sugar Analysis Columns...............176, 193, 199, 228-234, 245
Sumichiral OA Chiral Columns.............................................326
Sumika Chemical Analysis Service, Ltd. Columns................326
Supercritical Fluid Chromatography (SFC).................... 327-333
Superspher HPLC Cartridges...............................................209
Suppressed Mode Anion Analysis Column............................248
Sure-Fit Connector..............................................................364
Sure-Lok Coupler (PEEK)...............................................18, 363
Sure-Lok Fingertight Male Nut Fitting (PEEK).......................363
Sure-Lok Fitting Tightening Tool..........................................361
Sure-Lok High Pressure Male Nut Fitting (PEEK)..................361
Switching Valve/Injector......................................................376
Synergi Fusion-RP.................249, 252-253, 258-259, 342-343,
350-351
Synergi HPLC Column Selectors..........................................358
Synergi HPLC Columns................................................ 249-259
Synergi HPLC Fluid Processors............................................358
Synergi HST........................................................................258
Synergi Hydro-RP..........249, 254-255, 258-259, 342-343, 348,
350-351
Synergi Max-RP............ 249, 256-259, 339, 342-343, 347-351
Synergi Polar-RP........... 249-251, 258-259, 331-333, 342-343,
347-348, 350-351
Syringe and Adapter Kit, Strata SPE.......................................59
Syringe and Injector Accessories.........................................378
Syringe, Disposable Plastic............................................12, 369
Syringe Filter Selection Guide................................................10
Syringe Filters................................................................. 10-12
Syringe Filters, Sterile...........................................................12
Syringes, GC.................................................................108-111
Syringes, HPLC............................................................ 368-370
Syringe Needle Gauge Dimensions......................................108
Syringe Needle / Needle Connection Styles..........................108
Syringe Needle Point Styles.................................................108
System Test Kit, HPLC.........................................................355
UHPLC Columns.......................................................... 265-276

Aeris...........................................................................272
Clarity Oligo-MS...........................................................275
Kinetex........................................................................267
UHPLC Connectivity Kit........................................................362
Ultracarb HPLC Columns.....................................................262
Ultra-High Performance LC Fittings.............................. 361-362
Ultrasphere HPLC Columns..................................................262
Ultremex HPLC Columns......................................................262
Ultron ES Chiral Columns.....................................................325
Unions, GC............................................................................99
Unions, HPLC.............................................................. 360-364
Urea Type Chiral Columns............................................ 314-316
USP Methods, GC Column Selection......................................66
USP Methods, HPLC Column Selection........................ 152-153
USP Methods, Luna Column Selection.................................193
v
Vacuum Manifold, 96-Well Plate............................................58
Vacuum Manifold Accessories...............................................59
Vacuum Manifold Replacement Parts............................... 58-59
Vacuum Manifolds, SPE................................................... 57-59
Valve Caps for GL45 Threaded Bottles...................................14
Valve Repair Kit, Fluid Processor..........................................358
Valve Stators.......................................................................378
Valves......................................................................... 375-378
Varian GC Systems, Parts for............................... 106, 107, 109
Verex Vials....................................................................... 21-34
Vespel/Graphite Ferrules............................................. 102-103
Vial Compatibility Chart.........................................................34
Vial Inserts................................................................ 29, 30, 31
Vial Kits................................................................26-29, 31-33
Vials................................................................................ 21-34
Vials, Levels of Certification............................................. 22-23
Vials, Specialty, 12 x 32 mm..................................................24
Vial, Septa.............................................................................30
VICI Gas Syringes................................................................369
VOA / ASE Vial Kits.................................................................33
Vydac HPLC Columns..........................................................262
WAX GC Columns, Zebron................................................88, 89

Website......................................................... Inside Back Cover
Well Plate, 96-, Strata............................................... 36, 48-55
Well Plate, 96-, Strata-X.......................................36, 38, 40-46
Well Plate, 96-, Vacuum Manifold..........................................58
Whatman HPLC Columns.....................................................263
Whelk-O 1 Chiral Cartridges................................................324
Wide-Mouth Vials..................................................................25
Wrench, Fitting, for Rheodyne Valves...........................373, 378
Wrench, SecurityGuard........................................................236
Wrenches............................................................................373
Appendices
Phenomenex
Zebron GC Columns......................................................... 61-95

ZB-1...............................................................................71
ZB-1HT Inferno...............................................................73
ZB-1ms..........................................................................72
ZB-1XT SimDist..............................................................74
ZB-5...............................................................................75
ZB-5HT Inferno......................................................... 78-79
ZB-5ms..........................................................................77
ZB-5MSi.........................................................................78
ZB-35.............................................................................82
ZB-35HT Inferno.............................................................83
ZB-50.............................................................................84
ZB-624...........................................................................85
ZB-1701.........................................................................86
ZB-1701P.......................................................................87
ZB-BAC1.........................................................................95
ZB-BAC2.........................................................................95
ZB-Bioethanol.................................................................91
ZB-Drug-1......................................................................94
ZB-FFAP.........................................................................90
ZB-MultiResidue (MR)-1........................................... 92-93
ZB-MultiResidue (MR)-2........................................... 92-93
ZB-MultiResidue Column Kits..........................................93
ZB-WAX..........................................................................88
ZB-WAXplus....................................................................89
ZB-XLB...........................................................................80
ZB-XLB-HT Inferno..........................................................81
Zebron GC Columns Applications................................. 115-143
Zebron GC Columns, Fast......................................................69
Zebron GC Columns Five (5) Inch Cage..................................70
Zebron GC Columns MS Certification...................................382
Zebron GC Columns Test Mixtures.........................................98
Zebron Inferno GC Columns............................73, 78-79, 81, 83
Zero Dead-Volume Union, PEEK...........................................364
Zero Dead-Volume Union, Stainless Steel............................ 361
Z-Guard Columns..................................................................68
ZORBAX HPLC Columns.......................................................264
PRODUCT INDEX
Tall-Boy Vacuum Manifold.....................................................58

Tapered FocusLiners.................................................... 104-106
Teflon (PTFE) Tubing......................................................16, 373
Temperature Controllers, HPLC Column...............................357
Terms and Conditions of Sale..............................................407
Terry-Tool Tubing Cutter.......................................................373
Test Kit, HPLC System..........................................................355
Test Mixes, Chiral................................................................366
Test Mixes, GC.......................................................................98
Test Mixes, HPLC......................................................... 365-366
ThermaSphere TS-130 HPLC Column Heater.......................357
ThermaSphere TS-430 HPLC Chiller/Heater.........................357
Thermo Scientific GC Systems, Parts for......................106, 110
Tightening Tool, Sure-Lok Fitting..........................................361
Timeline, Company........................................ Inside Front Cover
TMS Endcapped C12 Column (Synergi Max-RP)...................249,
256-259, 339, 342-343, 347-351
Tosoh Bioscience LLC HPLC Columns...........................260-261
Trademarks.........................................................................406
Traps, Combination Oxygen/Moisture...................................112
Traps, Hydrocarbon..............................................................112
Traps, Indicating Glass Moisture..........................................112
Traps, Indicating Oxygen......................................................112
Troubleshooting GC Column Installation Problems................382
TSKgel Columns...........................................................260-261
Tubes, SPE...................................................................... 36-56
Tubing Applications Table.....................................................371
Tubing Compatibility Table...................................................371
Tubing Cutters.....................................................................373
Tubing Diameter Table.........................................................371
Tubing, Kit, PEEK.................................................................372
Tubing Material Maximum Temperature Ratings (C) Table... 371
Tubing Selection..................................................................371
Tubing, Capillary, PEEK, HPLC......................................248, 372
Tubing, HPLC..................................................16, 248, 371-374

Tubing, HPLC Column Assemblies, Empty............................374
Tubing, HPLC, PEEK......................................248, 362, 371-373
Tubing, HPLC, Stainless Steel....................................... 371-374
Tubing, HPLC, Teflon......................................................16, 373
Tubing, PEEKsil............................................................362, 372
TWIN (Two-In-One) Technology.................................... 168-175
TWIN-NX Technology................................................... 168-175
y
Y-connector (splitter).............................................................99
405
Appendices
TRADEMARKS
Trademarks
406
ACE...........................Advanced Chromatography Technologies

ACQUITY.............................................................. Waters Corp.
AD-H.....................................DAICEL Chemical Industries, Ltd.
Adsorbosphere..................................... Alltech Associates, Inc.
Aeris............................................................Phenomenex, Inc.
Alliance............................................................... Waters Corp.
Aminex....................................................Bio-Rad Laboratories
AQUA...........................................................Phenomenex, Inc.
Arylene Matrix Technology. ..........................Phenomenex, Inc.
Asahipak....................................................Showa Denko, K.K.
AutoSystem. ..................................................PerkinElmer, Inc.
Axia.............................................................Phenomenex, Inc.
Bakerbond.......................................... Mallinckrodt Baker, Inc.
BEH Technology.................................................. Waters Corp.
BioSep. ........................................................Phenomenex, Inc.
Bio-Sil.....................................................Bio-Rad Laboratories
Bondagel/Bondagel........................................... Waters Corp.
Bondapak/Bondapak. ...................................... Waters Corp.
Bondclone....................................................Phenomenex, Inc.
Bond Elut. .............................................................. Varian, Inc.
Bond Elut Certify..................................................... Varian, Inc.
BTO...........................Chromatography Research Supplies, Inc.
Capcell Pak.................................................. Shiseido Co., Ltd.
Captiva................................................................... Varian, Inc.
Ceramospher................................................ Shiseido Co., Ltd.
ChiraDex. ............................Merck KGaA, Darmstadt, Germany
CHIRALCEL............................DAICEL Chemical Industries, Ltd.
CHIRALPAK............................DAICEL Chemical Industries, Ltd.
Chirex.................................................................... Chirex, Inc.
Chromolith. .........................Merck KGaA, Darmstadt, Germany
ChromTRAC.......................................................Rheodyne L.P.
Clarity. .........................................................Phenomenex, Inc.
Clarus. ...........................................................PerkinElmer, Inc.
Columbus. ...................................................Phenomenex, Inc.
ComboTrap...................................................Phenomenex, Inc.
Cool-Lock....................................................Phenomenex, Inc.
Cosmosil..........................................................Nacalai Tesque
CTFE . ....................................E.I. du Pont de Nemours and Co.
Curosil.........................................................Phenomenex, Inc.
Cyrolite.......................................................... CY/RO Industries
Degassex.....................................................Phenomenex, Inc.
Delrin.....................................E.I. du Pont de Nemours and Co.
Develosil. ...............................................Nomura Chemical Co.
Discovery....................................Sigma-Aldrich Biotechnology
Easy Seals...................................................Phenomenex, Inc.
Engineered Self Cross-linking(ESC)..............Phenomenex, Inc.
EnviroSep. ...................................................Phenomenex, Inc.
ESC..............................................................Phenomenex, Inc.
EZ:faast. ......................................................Phenomenex, Inc.
FilterSys.......................................................Phenomenex, Inc.
Florisil.................................................................U.S.Silica Co.
FOCUS........................................ Thermo Fisher Scientific Inc.
FocusLiner........................................................................SGE
forte..................................................................................SGE
GASTIGHT............................................................ Hamilton Co.
Gemini. ........................................................Phenomenex, Inc.
Genesis................................................ Alltech Associates, Inc.
Giga.............................................................Phenomenex, Inc.
Glass Infusion. .............................................Phenomenex, Inc.
Guardian......................................................Phenomenex, Inc.
GuideRight...................................................Phenomenex, Inc.
Hibar...................................Merck KGaA, Darmstadt, Germany
HP..................................................... Agilent Technologies Inc.
Hypercarb...................................... Thermo Hypersil-Keystone
HyperClone..................................................Phenomenex, Inc.
Hypersil.......................................... Thermo Hypersil-Keystone
Hypersil GOLD................................ Thermo Hypersil-Keystone
IB-Sil. ..........................................................Phenomenex, Inc.
Impact.........................................................Phenomenex, Inc.
Inertsil...........................................................GL Sciences, Inc.
Inferno.........................................................Phenomenex, Inc.
ISOLUTE...................................................................... Biotage
IST ................................................................................ Biotage
Jupiter. ........................................................Phenomenex, Inc.
Kalrez.....................................E.I. du Pont de Nemours and Co.
Kinetex.........................................................Phenomenex, Inc.
Kromasil........................................................... Eka Chemicals
KrudKatcher.................................................Phenomenex, Inc.
LiChroCART.........................Merck KGaA, Darmstadt, Germany
LiChrosorb...........................Merck KGaA, Darmstadt, Germany
LiChrospher.........................Merck KGaA, Darmstadt, Germany
Luer-LOK..........................................Becton Dickinson and Co.

Luna.............................................................Phenomenex, Inc.
Lux...............................................................Phenomenex, Inc.
Magnum........................................................... Whatman, Inc.
Maxsil..........................................................Phenomenex, Inc.
MercuryMS..................................................Phenomenex, Inc.
Micro-B............................................................... Whatman, Inc.
MICROLITER........................................................ Hamilton Co.
Microseal...............................................Merlin Instrument Co.
Microtight................................................. Upchurch Scientific
MidBore.......................................................Phenomenex, Inc.
Modified MICROLITER. ........................................ Hamilton Co.
MultiPROBE....................................................PerkinElmer, Inc.
MultiResidue................................................Phenomenex, Inc.
MXT. ................................................................... Restek Corp.
NEXT............................................................Phenomenex, Inc.
Nova-Pak. ........................................................... Waters Corp.
Nucleodex......................................................Macherey-Nagel
Nucleogen......................................................Macherey-Nagel
Nucleosil. .......................................................Macherey-Nagel
Nylon 66.................................E.I. du Pont de Nemours and Co.
Oasis................................................................... Waters Corp.
OBD.................................................................... Waters Corp.
OD-H.....................................DAICEL Chemical Industries, Ltd.
OJ-H. ....................................DAICEL Chemical Industries, Ltd.
Oligo-MS. ....................................................Phenomenex, Inc.
Oligo-RP. .....................................................Phenomenex, Inc.
Oligo-WAX. ..................................................Phenomenex, Inc.
Onyx. ...........................................................Phenomenex, Inc.
Orbit. ...........................................................Phenomenex, Inc.
OTX. ............................................................Phenomenex, Inc.
OxiClear.................................................................... LabClear
Partisil...................................................................... Whatman
PartiSphere. ............................................................. Whatman
PEEK....................................................................... Victrex plc
PEEKsil. ............................................................................SGE
PerkinElmer....................................................PerkinElmer, Inc.
PHAT Phase. ........................................... Quadrex Corporation
Phenex. .......................................................Phenomenex, Inc.
Phenogel. ....................................................Phenomenex, Inc.
PhenoBlue. ..................................................Phenomenex, Inc.
PhenoGreen.................................................Phenomenex, Inc.
PhenoGrey...................................................Phenomenex, Inc.
PhenoRed....................................................Phenomenex, Inc.
PhenoSphere. ..............................................Phenomenex, Inc.
pH-LC..........................................................Phenomenex, Inc.
PLgel..............................................Polymer Laboratories, Ltd.
PLRP-S...........................................Polymer Laboratories, Ltd.
Polyglas............................................................................SGE
Polygosil.........................................................Macherey-Nagel
PolymerX. ....................................................Phenomenex, Inc.
PolySep. ......................................................Phenomenex, Inc.
POLYWAX. ................................................... Baker Hughes Inc.
Porasil/Porasil................................................. Waters Corp.
Pressure-Lok.......................................VICI Precision Sampling
PrimeSphere................................................Phenomenex, Inc.
Prodigy........................................................Phenomenex, Inc.
Prominence...........................................Shimadzu Corporation
Protein-Pak......................................................... Waters Corp.
PRP..................................................................... Hamilton Co.
PTFE. .....................................E.I. du Pont de Nemours and Co.
Purospher. ..........................Merck KGaA, Darmstadt, Germany
Pursuit. ......................................................................... Varian
QSP. ............................................................Phenomenex, Inc.
RACII................................................................. Whatman, Inc.
RCX. ................................................................... Hamilton Co.
Resolve............................................................... Waters Corp.
Resolvosil.......................................................Macherey-Nagel
Rezex...........................................................Phenomenex, Inc.
RheBuild. .......................................IDEX Health & Science LLC
RheFlex..........................................IDEX Health & Science LLC
RP-Desalting................................................Phenomenex, Inc.
Rtx...................................................................... Restek Corp.
Rx. ................................................... Agilent Technologies, Inc.
Rxi. ..................................................................... Restek Corp.
Santoprene........................................Exxon Mobil Corporation
SecurityGuard..............................................Phenomenex, Inc.
Selectosil.....................................................Phenomenex, Inc.
Sep-Pak.............................................................. Waters Corp.
Sepra...........................................................Phenomenex, Inc.
Septa BTO.................Chromatography Research Supplies, Inc.
Shimadzu..............................................Shimadzu Corporation
Shodex........................................................Showa Denko K.K.
SilTite. ..............................................................................SGE
Solvecon........................................................... Whatman, Inc.
Solvent Saver...............................................Phenomenex, Inc.
Solvent Shielding Technology.......................Phenomenex, Inc.
SolventTrak.................................Axxiom Chromatography Inc.
SOURCE............................................................ GE Healthcare
SphereClone................................................Phenomenex, Inc.
Spherex.......................................................Phenomenex, Inc.
Spherisorb........................................................... Waters Corp.
StableBond....................................... Agilent Technologies, Inc.
Stabilwax. ........................................................... Restek Corp.
STAR-ION.....................................................Phenomenex, Inc.
Strata...........................................................Phenomenex, Inc.
Strata-X.......................................................Phenomenex, Inc.
Stx. ..................................................................... Restek Corp.
Styragel/Styragel. ........................................... Waters Corp.
Sugar-Pak........................................................... Waters Corp.
Sumichiral OA.............Sumika Chemical Analysis Service, Ltd.
SunFire............................................................... Waters Corp.
Supelcosil...................................Sigma-Aldrich Biotechnology
Superspher. ........................Merck KGaA, Darmstadt, Germany
Sure-Fit. ............................................... Isolation Technologies
Sure-Lok......................................................Phenomenex, Inc.
Swagelok........................................................... Swagelok Co.
Symmetry. .......................................................... Waters Corp.
SymmetryShield. ................................................ Waters Corp.
Synergi.........................................................Phenomenex, Inc.
Tall-Boy. ......................................................Phenomenex, Inc.
Taxol. ................................................Bristol-Myers Squibb Co.
Teflon.....................................E.I. du Pont de Nemours and Co.
Tefzel. ....................................E.I. du Pont de Nemours and Co.
ThermaSphere.............................................Phenomenex, Inc.
Thermo. ...................................... Thermo Fisher Scientific Inc.
Tipp-Ex. .............................................. Tipp-Ex GmbH & Co KG
TRACE. ....................................... Thermo Fisher Scientific Inc.
Triophilic......................................................Phenomenex, Inc.
TSKgel. ....................................................... Tosoh Corporation
TWIN-NX......................................................Phenomenex, Inc.
TWIN Technology . .......................................Phenomenex, Inc.
Two-In-One Technology .................................Phenomenex, Inc.
Ultracarb......................................................Phenomenex, Inc.
Ultrasphere. ...........................................Beckman Coulter, Inc.
Ultrastyragel. ...................................................... Waters Corp.
Ultremex......................................................Phenomenex, Inc.
Ultron........................................... Shinwa Chemical Industries
UPLC................................................................... Waters Corp.
Valco........................................VICI Valco Instruments Co., Inc.
Verex...........................................................Phenomenex, Inc.
Vespel....................................E.I. du Pont de Nemours and Co.
Vydac................................................... Alltech Associates, Inc.
Waters. ............................................................... Waters Corp.
WAXplus.......................................................Phenomenex, Inc.
Whelk-O..........................................................................Regis
WISP................................................................... Waters Corp.
Wite-Out...................................................... BIC Corp. USA Inc.
XBridge............................................................... Waters Corp.
XDB.................................................. Agilent Technologies, Inc.
XTerra. ................................................................ Waters Corp.
Z-Guard.......................................................Phenomenex, Inc.
Zebron. ........................................................Phenomenex, Inc.
ZORBAX. .......................................... Agilent Technologies, Inc.
Phenomenex
Terms and Conditions of Sale

Terms and Conditions vary by country. To view the terms and conditions specific to your country, please
visit http://www.phenomenex.com/Home/TermsAndConditionsOfSale
Terms are net 30 days from invoice date with approved credit. If your credit has not been established with Phenomenex, terms
may be payment in advance or C.O.D. All shipments are F.O.B. factory. Items are shipped by a fast/standard method unless
otherwise specified, with shipping charges and insurance prepaid and added as a separate charge on your invoice. A 1.5 % per
month service charge is added to delinquent accounts. In the event it becomes necessary for Phenomenex to incur collection
costs or institute a suit to collect any amount due and payable, the customer (Buyer) agrees to pay such additional collection
costs, charges, and expenses, including attorneys fees if the account is placed in the hands of an attorney or an agency for
collection. Prices are subject to change without notice.
Conditions
The information published in this catalogue is, to the best of our knowledge, correct and accurate but is not guaranteed to be
so. Phenomenex assumes no responsibility with respect thereto, and has not verified the values or specifications stated experimentally and does not guarantee their accuracy. The sale of any product by Phenomenex does not waive any patent restrictions
connected with those products.
Changes
Phenomenex reserves the right to change product specifications, quantities, designs or prices without notice and without liability
for such changes.
Order Cancellation
Cancellation of orders is subject to approval by Phenomenex. A minimum of 20 % restocking/service charge will be applied.
Damage Claims
Should you receive damaged goods, it is imperative that you save all packing materials for inspection by the carrier. Once the
goods are turned over to the carrier by us, they become your responsibility. While we will assist you, all claims should be reported
to the carrier immediately. This is essential. Failure to do so makes you, the customer, liable for payments for those goods should
damage claims be rejected by the carrier. All claims must be made within 7 days of receipt of goods.
Returns
No returns will be accepted without prior authorization, and are subject to approval by Phenomenex. If, for any reason, it is
necessary to return goods to us, please contact us for forwarding instructions. Upon approval, Phenomenex will issue a Return
Authorization number and a Hazardous Material Certification Fax Form. A signed copy of this form must be faxed to Phenomenex
and must be on file before the items can be accepted for return. This procedure will prevent delays and enable us to resolve the
situation to your satisfaction. Phenomenex is not liable for goods returned without authorization. Returns must be sent through
a traceable carrier.
Duplication of Orders
Goods ordered in error or duplicated because mailed-in order is not clearly marked "CONFIRMING" will be subject to a minimum
20 % restocking charge, if approved by Phenomenex.
Product Limitations
Warranty
Phenomenex products are warranted to meet the stated performance and quality and to be free of defects in material and
workmanship. They are not warranted, nor does Phenomenex assume liability, if misused. No other warranty or representation is implied or expressed by Phenomenex for its products with respect to merchantability, fitness for a particular purpose, or any other matter. Phenomenex shall not under any
circumstances be liable for any incidental, consequential or compensatory damage arising from
the use of, or in conjunction with, its products. The maximum liability that can be assumed by Phenomenex for
breach of warranty shall be the invoice price of the product.
Specific Warranties on HPLC and GC Columns
Maximum warranty period is limited to 90 days unless previously agreed upon. However, COLUMNS MAY NOT
BE RETURNED FOR REFUND OR CREDIT AFTER 45 DAYS AND WITHOUT PRIOR AUTHORIZATION.
Appendices
All columns must be tested upon receipt and all deficiencies must be reported to Phenomenex no later than
15 days after the date of receipt of the column.
Phenomenex warrants its quality columns in accordance with the following terms and conditions. Phenomenex will repack,
replace, or refund charges on any column (at our discretion), at no cost if a column fails to perform satisfactorily. Columns being
returned must have prior return authorization granted by Phenomenex. Defective products must be accompanied by a written
explanation of failure. Approval is subject to the following exclusions:
TERMS AND CONDITiONS OF SALE
The products offered in this catalog and Phenomenex Analyte Specific Reagentproducts are not intended for clinical use. Because
they are not intended for clinical use, no claim or representation is made or intended for their clinical use (including, but not limited
to diagnostic, prognostic, therapeutic or blood banking). It is the users responsibility to validate the performance of Phenomenex
products for any particular use, since the performance characteristics are not established. Phenomenex products may be used in
clinical diagnostic laboratory systems after the laboratory has validated their complete system as required by the Clinical Laboratory
Improvements Amendments of 1988 (CLIA 88) regulation in the U.S. or equivalent in other countries.
Removal of HPLC column end-fittings automatically voids column warranty.

Un-wrapping or removing the GC column from its cage automatically voids warranty.
Column performance warranty is limited to the conditions of the original test chromatogram.
Physical damage to the column due to misuse, abuse, or mishap, including mechanical shock.
Chemical damage to the stationary phase or support material due to operation at incorrect chemical conditions,
temperatures, or pressures.
Phenomenex
407
Now Accepting Free Labor

Why? Because we are so cheap! Just kidding. In all seriousness, wed love for you to share your
thoughts, ideas, and concepts on how we might better serve you. We want to hear what new products youd like to have, research support you need, and collaboration projects youre interested in
pursuing. The more ingenious brains like yours that we have, the stronger we ALL are. So talk to us!
Were ready to listen.
Email us at:
BRAINS@phenomenex.com
Were CoolArent We?

If you made it this far, please put down your drink, and let us know how you really feel about Phenomenex!
Select the option below that best describes your opinion of us:
1) I love you guys, but in the platonic way. I dont typically date separation science companies.
2) Youre pretty cool, but I have reservations. No really, I have dinner at 6 pm so I have to go.
3) Im neutral, because I have an equal amount of protons and electrons.
4) Youve gone mad. And so have your scientists.
5) I really think that ___________________________________________________________________

Share your response and have a little fun at
www.phenomenex.com/CURIOUS
Well send you a little something for your efforts!
At the end of the day, nothing is more important than making sure youre happy.
And so, we wish you a good day and a great year full of adventure, laughter, and success!
Did you miss all the fun?

No, you are not having a bad dream. Just go back to the first page and this will all make more sense!
408
Phenomenex
Best Website in the Industry!

And thats no joke! The Web Marketing Association said so. We just won the 2011
Best-of-Industry Award, and once you see it for yourself, we know youll agree.
The newly redesigned Phenomenex website is not only good-looking (sexy, even),
but its functional, too.
2011
The Web Marketing Associations annual WebAward

program is the premier website award competition that
names the best Websites in 96 industries while setting
the standard of excellence for all website development.
www.
phenomenex
.com
2012
Sit back. Grab a Gin and Tonic Sorry,
we meant a coffee, and enjoy a grand tour!

Whether youre curious about how we got started, or
want to contribute to our joke wall, you can visit us in
person without ever leaving the safety and security
of your chair.
And dont worry, we didnt fill the pages with rambling
commentary (even though you must really like it if you
have read this far). Instead, its filled with really fun
stories, images, and information that we think youll
find interesting enough to keep exploring.
2013
Free app for your mobile:

http://www.gettag.mobi
www.phenomenex.com/corporate
2014
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2012 Phenomenex, Inc. All rights reserved.
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Lets face it.

2012 Phenomenex, Inc. All rights reserved.

Buildings 1, 2, 3, 5, 6, 7, and 10 (shown). Present day.

Mr. Mahjoors Garage. Circa 1982

InventX (GC Manufacturing)

For the sake of humanity, visit page 60.

Sample Preparation / spe

GC Columns and Accessories

Amino Acids...........................................................................278 282

Beliefs and Principles

The oneness of humankind

We are as passionate about science as

Technical Manager, PhenoLogix

HPLC | UHPLC | GC | GC/MS | LC/MS/MS | SPE |

Small Scale Purification (< 2 g)

Get Started Today We Make it Easy

Speak with one of our friendly PhenoLogix team members or

Tell Us About The Project

Provide information on your project such as compound

Most Services are Free

Tell Us When to Start

Approve our proposal and well get to work on your project!

Open Lines of Communication

A detailed report is sent to you, including full method,

Biocompatible PEEK.................................................................... 17-18

Stainless Steel.............................................................................. 17-18

Mobile Phase Filtration Accessories........................................................ 14

Mobile Phase Filtration System............................................................... 13

Solvent Pickup Adapter........................................................................... 14

Rapid filtration of HPLC and GC samples prior to analysis

More consistent, reproducible results

Syringe Filter Selection Guide

Broad chemical compatibility

100 % integrity tested

FILTRATION - Sample handling

For sample and solvent filtration prior to

1. Choose filter diameter based on sample volume

Recommended Filter Pore Size

Viscous samples or samples containing high levels of particulate matter.

Glass Fiber Filter

Syringe Filters (contd)

Phenex Syringe Filters

Tip: Try a Sample Pack!

Sample handling - FILTRATION

1. Larger quantity purchases at significant savings are available.

8. Additional dimensions and membrane types are available. Please contact

3. Housing material is methacrylate butadiene styrene (MBS) polymerisate.

Above syringe filters are non-sterile. Housing is made of

Sterile syringe filters from Phenomenex are ready-to-use, individually

Disposable Centrifugal Filter

Disposable Syringes / Sterile Syringe Filters / centrifugal filters

| FILTRATION - Sample handling

High recovery for small samples

Phenex disposable centrifugal filter units provide a convenient

All-Plastic Disposable Syringes

Above centrifugal filter units are non-sterile.

WARNING: The apparatus should be

Mobile Phase FILTRATION system

Recommended filter membranes: Nylon 66 is a highly resistant

Mobile Phase - FILTRATION

Designed for rapid filtration of particulate matter from HPLC

For compatible Solvent Pickup Adapter, see p. 14