1)Secuencia de ADN ATGTGCGACCATACCAAGCTGAAAATACTG CATCCCGTCTGATCTGCACAGTCAAGCAGC TTAGGGCCCAGTCAGTAGTGCGGTGGGGGA CCATGCGCGAACATTGTGGTGTTGCACTTA TGTGCGACCATACCAAGCTGAAAATACTGC ATCCCGTCTGATCTGCACAGTCAAGCAGCT TAGGGCCCAGTCAGTAGTGCGGTGGGGGAC CATGCGCGAACATTGTGGTGTTGCACTTAT GTGCGACCATACCAAGCTGAAAATACTGCATCCCGTCTGATCTGCACAGTCAAGCAGCTT AGGGCCCAGTCAGTAGTGCGGTGGGGGACC ATGCGCGAACATTGTGGTGTTGCACTTATG TGCGACCATACCAAGCTGAAAATACTGCAT CCCGTCTGATCTGCACAGTCAAGCAGCTTA

GGGCCCAGTCAGTAGTGCGGTGGGGGACCA TGCGCGAACATTGTGGTGTTGCACTT 2.2.A)Secuencia reversa TTCACGTTGTGGTGTTACAAGCGCGTACCA GGGGGTGGCGTGATGACTGACCCGGGATTC GACGAACTGACACGTCTAGTCTGCCCTACGTCATAAAAGTCGAACCATACCAGCGTGTAT TCACGTTGTGGTGTTACAAGCGCGTACCAG GGGGTGGCGTGATGACTGACCCGGGATTCG ACGAACTGACACGTCTAGTCTGCCCTACGT CATAAAAGTCGAACCATACCAGCGTGTATT CACGTTGTGGTGTTACAAGCGCGTACCAGGGGGTGGCGTGATGACTGACCCGGGATTCGA CGAACTGACACGTCTAGTCTGCCCTACGTC ATAAAAGTCGAACCATACCAGCGTGTATTC ACGTTGTGGTGTTACAAGCGCGTACCAGGG GGTGGCGTGATGACTGACCCGGGATTCGAC GAACTGACACGTCTAGTCTGCCCTACGTCA TAAAAGTCGAACCATACCAGCGTGTA 2.B)Secuencia complementaria AAGTGCAACACCACAATGTTCGCGCATGGTCCCCCACCGCACTACTGACTGGGCCCTAAGCTGCTTGAC TGTGCAGATCAGACGGGATGCAGTATTTTCAGCTTGGTATGGTCGCACATA AGTGCAACACCACAATGTTCGCGCATGGTC CCCCACCGCACTACTGACTGGGCCCTAAGC TGCTTGACTGTGCAGATCAGACGGGATGCA GTATTTTCAGCTTGGTATGGTCGCACATAA GTGCAACACCACAATGTTCGCGCATGGTCC CCCACCGCACTACTGACTGGGCCCTAAGCT GCTTGACTGTGCAGATCAGACGGGATGCAG TATTTTCAGCTTGGTATGGTCGCACATAAG TGCAACACCACAATGTTCGCGCATGGTCCC CCACCGCACTACTGACTGGGCCCTAAGCTG CTTGACTGTGCAGATCAGACGGGATGCAGT ATTTTCAGCTTGGTATGGTCGCACAT 2.C)Trncrito de ARN AUGUGCGACCAUACCAAGCUGAAAAUACUG CAUCCCGUCUGAUCUGCACAGUCAAGCAGC UUAGGGCCCAGUCAGUAGUGCGGUGGGGGA CCAUGCGCGAACAUUGUGGUGUUGCACUUA UGUGCGACCAUACCAAGCUGAAAAUACUGC AUCCCGUCUGAUCUGCACAGUCAAGCAGCU UAGGGCCCAGUCAGUAGUGCGGUGGGGGAC CAUGCGCGAACAUUGUGGUGUUGCACUUAU GUGCGACCAUACCAAGCUGAAAAUACUGCA UCCCGUCUGAUCUGCACAGUCAAGCAGCUU AGGGCCCAGUCAGUAGUGCGGUGGGGGACC AUGCGCGAACAUUGUGGUGUUGCACUUAUG UGCGACCAUACCAAGCUGAAAAUACUGCAU CCCGUCUGAUCUGCACAGUCAAGCAGCUUA GGGCCCAGUCAGUAGUGCGGUGGGGGACCA UGCGCGAACAUUGUGGUGUUGCACUU

2.D)Protena codificada.Cantas solucins posibles hai para a secuencia problema?Porque? Marco de lectura RF1 MCDHTKLKILHPV*SAQSSSLGPSQ*CGGGPCANIVVLHLCATIPS*KYCIPSDLHSQAA*GPVSSAVGDHAR TLWCCTYVRPYQAENTASRLICTVKQLRAQSVVRWGTMREHCGVALMCDHTKLKILHPV*SAQSSSLGPS Q*CGGGPCANIVVLH Marco de lectura RF2 CATIPS*KYCIPSDLHSQAA*GPVSSAVGDHARTLWCCTYVRPYQAENTASRLICTVKQLRAQSVVRWGTM REHCGVALMCDHTKLKILHPV*SAQSSSLGPSQ*CGGGPCANIVVLHLCATIPS*KYCIPSDLHSQAA*GPVS SAVGDHARTLWCCT Marco de lectura RF3 VRPYQAENTASRLICTVKQLRAQSVVRWGTMREHCGVALMCDHTKLKILHPV*SAQSSSLGPSQ*CGGGP CANIVVLHLCATIPS*KYCIPSDLHSQAA*GPVSSAVGDHARTLWCCTYVRPYQAENTASRLICTVKQLRAQS VVRWGTMREHCGVAL Hai estas tres posibles solucins para a secuencia.Temos varias opcins dado que non sabemos en que nucletido comeza a lectura,pode ser tanto no primeiro,no segundo como no terceiro. Como unha molcula de ADN est composta por das cadeas,as posibilidades totales sern seis.

e)Estadsticas de secuencia e.1)Porcentaje de nucleotidos A: 23.52%(112nucletidos) G: 28.57%(136nucletidos) C: 25.21%(120nucletidos) T: 22.68%(108nucletidos)

e.2)Porcentaje de dmeros: 94,71% AA: AT: CG: GC: TA: TT: 24 dmeros 5.04% 28 dmeros 20 dmeros 44 dmeros 19 dmeros 16 dmeros 5.88% 4.2% 9.24% 3.99% 3.36% AC: CA: CT: GG: TC: 28 dmeros 5.88% AG: CC: GA: GT: TG: 32 dmeros 28 dmeros 20 dmeros 40 dmeros 6.72% 5.88% 4.2% 8.4%

48 dmeros 10.08% 24 dmeros 32 dmeros 20 dmeros 5.04% 6.72% 4.2%

52 dmeros 10.92%

e.3)Porcentaje de tripletes AAA: AAG: ACA: ACG: AGA: AGG: ATA: ATG: CAA: CAG: CCA: CCG: CGA: CGG: CTA: CTG: GAA: GAG: GCA: GCG: GGA: GGG: GTA: GTG: TAA: TAG: 8 tripletes 1.68% 8 tripletes 1.68% 8 tripletes 0 tripletes 0 tripletes 1.68% 0% 0% AAC: AAT: ACC: ACT: AGC: AGT: ATC: ATT: CAC: CAT: CCC: CCT: CGC: CGT: CTC: CTT: GAC: GAT: GCC: GCT: GGC: GGT: GTC: GTT: TAC: TAT: 4 tripletes 0.84% 4 tripletes 0.84% 12 tripletes 2.52% 8 tripletes 1.68% 12 tripletes 2.52% 16 tripletes 3.36% 8 tripletes 1.68% 4 tripletes 0.84% 8 tripletes 1.68% 16 tripletes 3.36% 8 tripletes 1.68% 0 tripletes 0% 4 tripletes 0.84% 4 tripletes 0.84% 0 tripletes 0% 8 tripletes 1.68% 8 tripletes 1.68% 4 tripletes 0.84% 4 tripletes 0.84%

4 tripletes 0.84% 8 tripletes 1.68% 8 tripletes 1.68% 8 tripletes 1.68% 16 tripletes 16 tripletes 3.36% 3.36%

4 tripletes 0.84% 8 tripletes 1.68%

4 tripletes 0.84% 0 tripletes 0%

16 tripletes 3.36% 8 tripletes 1.68% 0 tripletes 0% 16 tripletes 3.36%

16 tripletes 3.36% 4 tripletes 0.84% 16 tripletes 3.36% 4 tripletes 0.84% 20 tripletes 0 tripletes 4.2% 0%

8 tripletes 1.68% 4 tripletes 0.84% 8 tripletes 1.68% 12 tripletes 2.52% 4 tripletes 0.84%

8 tripletes 1.68% 3 tripletes 0.63%

8 tripletes 1.68%

TCA: TCG: TGA: TGG: TTA: TTG:

8 tripletes 1.68% 0 tripletes 0%

TCC: TCT: TGC: TGT: TTC: TTT:

4 tripletes 0.84% 8 tripletes 1.68% 24 tripletes 5.04% 12 tripletes 2.52% 0 tripletes 0% 0 tripletes 0%

8 tripletes 1.68% 8 tripletes 1.68% 7 tripletes 1.47% 8 tripletes 1.68%

3.)Enderezo utilizado http://www.bioxeo.com/moodle6/mod/resource/view.php?id=643